F R A U N H O F E R I N S T I T U T E F O R M O L E C U L A R B
I O L O G Y A N D A P P L I E D E C O L O G Y I M E
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IN VITRO TESTS1 Multi-well plate.
2 Macrophages in lesions of EAE
mice.
A wide variety of in vitro tests are available
within the Fraunhofer IME Project Group
Translational Medicine and Pharmacology.
Emphasis is laid on cellular signaling
processes, lipid metabolism, neuronal
cell responses and immune cell activity.
Cytotoxicity, mutagenicity, proliferation
and apoptosis assays are performed.
State-of-the art cellular, immunological,
biochemical, analytical, proteomic and
genomic techniques are employed.
Cellular models for receptor-binding
• G-protein-linked
• eicosanoid receptor binding
Cellular lipid metabolism
• sphingolipid metabolism, sphingosine
kinases, S1P lyase
• sphingosine -1-P receptor binding and
signaling
• eicosanoid metabolism
• endocannabinoid metabolism
Signaling assays
• calcium imaging of dorsal root ganglia
and spinal cord neurons
• chip assay
• miRNA analysis
Imaging
• Multi-Epitope-Ligand-Cartography
• immunofluorescence assays
Gene transfection
• lentiviral gene transfer
• in-situ hybridization
• cloning
• promoter-reporter gene assay
Single and co-cultures of immune
competent cells
• lymphocytes
• macrophages
• dendritic cells
• keratinocytes
• microglia
• synovial cells
• cytokine release and signalling
Other cell types
platelets,fibroblasts, renal mesenchymal
cells, podocytes, tumor cell lines, astrocytes,
neuroblastoma cell lines, primary neuronal
cultures
Fraunhofer Institute for
Molecular Biology and Applied
Ecology IME
Project Group for Translational
Medicine and Pharmacology TMP
Theodor-Stern-Kai 7
60596 Frankfurt am Main
Contact
Prof. Dr. Michael Parnham
Phone +49 69 6301-84234
[email protected]
www.ime.fraunhofer.de
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