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Inggrid Surono

Function of Indigenous probiotic fromdadih Enterococcus faeciumIS-27526in improving nutritional status and

suppress diarrhoea

Ingrid S Surono*, Fiona Koestomo, F

Riewpassa, Yulianasari and Netty Harnetty

*SEAMEO TROPMED RCCN University of IndonesiaSalemba Raya 6, Jakarta 10430 INDONESIA

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Dadih, Indonesian traditional

fermented raw buffalo milk of WestSumatra

Enterococcus faeciumEnterococcus faeciumISIS--27526 strain isolated27526 strain isolatedfrom Dadihfrom Dadih possess probiotic propertiespossess probiotic properties

Antagonisms against pathogens

Probiotic Properties

high

viability

acid and bile

toleranceadhesion

colonization

antimutagenic, hypocholesterolemic

Safety in food, clinical safety

processing, shelf life, probiotics stability

Immuno-modulatory

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TRADITIONAL WAY OF DADIH MAKINGTRADITIONAL WAY OF DADIH MAKING

Fresh raw unheated buffalo milk, poured into bamboo tubes

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Diarrhoea in a year globally

4000 milliondiarrhaea cases

1500 millionepisode of diarechildren youngerthan 5

1.8 million die

Every 3 seconds 1 child dies from diarrhoeaEvery 3 seconds 1 child dies from diarrhoea

70% cases related to contaminated food and drink70% cases related to contaminated food and drink

Cause of mortality of Indonesian

children

%

%

%

%

%

%

%

%

%

%

%

%

%

INFANTS UNDER FIVE CHILDREN

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2. Fekal Mikrobiota pada Feses Balita

Fecal Microbiota of children younger than five, before and after supplementationFecal Microbiota of children younger than five, before and after supplementationwith FPC biscuit with probiotic creamwith FPC biscuit with probiotic cream

Profile of fecal microbiota before after delta

1Fecal Aerobic bacteria (cfu/g)Log cfu/g

7.10 0.961.2 x 107

6.42 1.362.5 x 106

0.96 1.65

2Fecal Coliform (cfu/g)Log cfu/g

5.40 0.872.5 x 105

5.50 0.983.1 x 105

0.07 1.28

3Fecal lactic bacteria (cfu/g)Log cfu/g

4.48 3.193.0 x 104

6.35 0.702.2 x 106

1.86 3.41

4Fecal Anaerobic bacteria(cfu/g) Log cfu/g

6.38 0.922.5 x 106

6.29 1.231.9 x 106

0.09 1.15

5Fecal lactic Anaerobic (cfu/g)

Log cfu/g

5.61 0.71

4.0 x 105

6.23 0.99

1.5 x 106 0.62 0.45**

** significantly different before and after (P

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Morbidity of children younger than five subjects during 90days intervention study

Treatment Groups

Normal biscuit FPC biscuit FPC + Probioticcream

Day Day Day

0 30 60 90 0 30 60 90 0 30 60 90

Diarrhoea 5 2 3 1 3 2 4 2 6 4 0 0

High fever - 2 2 0 0 1 1 0 0 2 3 1

Commoncold

1 2 3 3 4 2 2 0 3 3 1 1

Dermatitis 1 1 0 2 0 0 0 0 0 0 0 0

Fever 4 2 2 1 4 2 2 0 5 0 0 0

Cough 5 1 2 1 6 2 2 0 6 0 0 0

Measle 0 0 0 0 1 0 0 0 0 0 0 0

Total 16 11 12 8 18 9 11 2 20 9 4 2

The improvement of Intestinal vili induces by probiotic LIS 10506 and LIS 20506A: Normal Vili ; B: Disruption o of vili caused by LPS E.coli ;

C: the improvement vili by LIS 10506 ; D: the improvement vili by LIS 20506

A B C D

(Reza Ranuh et al., 2008)

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Protein Intestinal's Brush Border

01000020000300004000050000600007000080000

Duodenum

Jejunum

Ileum

Duodenum

Jejunum

Ileum

Duodenum

Jejunum

Ileum

Duodenum

Jejunum

Ileum

Galectin-4 Myosin-1a Occludin ZO-1

concentration

in

ppm

LPS

Placebo

LIS10506

LIS20506

(Reza Ranuh et al., 2008)

Distribusi Ekspresi Protein Brush Border Usus

0

5

10

15

20

25

30

D J I D J I D J I D J I

G M ZO O

Protein Brush Border Usus

TingkatE

kspresi

KONTROL

LPS

LPS + LIS 10506

LPS + LIS 20506

p

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Gambar 21

-0.93

-0.75

-0.16

-0.93

-0.9

-0.82

-0.61

-1.39

-1.1

-1.28

-0.48-0.56

-1.6

-1.4

-1.2

-1

-0.8

-0.6

-0.4

-0.2

0

0 30 60 90

Days

Zscores

Biskuit Biskuit KPI Biskuit KPI+Probiotik

Z scores (weight/height)

Normal biscuit FPC biscuit FPC + probiotic cream

Effect of supplementation on nutritional status of

children less than five

Z scores

Before intervention After intervention

Normalbiscuit(n=35)

FPCbiscuit(n=32)

FPC +Probiotic

cream (n=35)

Biskuit(n=35)

Biskuit KPI(n=32)

Biskuit KPI+ Probiotik

(n=35)

n % n % n % n % n % n %

Weight/age

Normalunderweight

1223

34.365.7

626

18.881.2

431

11.488.6

2015

57.142.0

1418

43.856.2

1520

42.957.1

Height/age

NormalStunted

1421

4060

1814

56.243.8

1421

4060

1421

4060

1913

59.440.6

1916

54.345.7

Weight/height

NormalWasted

314

88.611.4

257

78.121.9

332

91.45.8

341

97.12.9

311

96.93.1

350

1000

Note : weight/age: Normal -2

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Human Study in Teluk NagaPublic Health Center -

Tangerang

Randomised Placebo Control Trial, Pre-post study

Two groups, n = 40 subjects, 90 days supplementationProbiotic E. faecium IS-27526 108 cfu/day

0.2200.231

0.2740.267

0.000

0.400

0.800

Placebo Probiotic

Group

Consentration(mg/ml)

0 d 90 d

Total Serum IgA

Total serum IgA (mg/ml), before and after intervention

Placebo : maltodextrinProbiotic : 108 cfu/day Enterococcus faeciumIS-27526

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So, which bacteria did you eat today?