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DNA determines your unique characteristics. A Review…
• DNA is the instructions for making proteins.• Proteins play a big role in everything you do and
determine most of your characteristics
Examples of Proteins
Hormones
Pigments
Muscle
Fibers
Enzymes
Cytoskeleton
Carrier Molecules
Membrane
Channels
Antibodies
Connective
DNA mRNA• Uracil replaces Thymine
• DO NOT say A turns into U. A codes for U• The DNA does not become or mutate into the
RNA
DNA nucleotide codes for RNA nucleotideAdenine (A) Uracil (U)Thymine (T) Adenine (A)Guanine (G) Cytosine (C)Cytosine (C) Guanine (G)
Where Does Transcription Begin?• At the promoter (for our simulations the
TATAA box)• Proteins called
transcription factorshelp RNA Polymerasebind to the DNA
Prokaryotes vs. Eukaryotes
Prokaryotes• No nucleus• Transcription and
translation happen relatively simulataneously
Eukaryotes• Transcription in nucleus• mRNA edited before
translation (beyond our scope)
• mRNA leaves nucleus for translation
How?• The sequence of mRNA nucleotides
determines the order of amino acids in the protein
• Every 3 nucleotides (codon) codes for one amino acid
Translation Details
• Occurs in the ribosome in both prokaryotes and eukaryotes• mRNA brings the code from
transcription• tRNA carries in amino acids one by
one• Protein is assembled
There are 3 different types of RNA:• fold differently =• different functions
1. MESSENGER (mRNA): carries message2. TRANSFER (tRNA): carries amino acids in
translation3. RIBOSOMAL (rRNA): makes up parts of the
ribosomes which are the sites of protein synthesis
How Does the tRNA “know” Which Amino Acid to Bring?
• The anticodon on the tRNA is complementary to the codon on the mRNA
Hydrogen bonds
The Start and Stop CodonsStart Codon• Begins translation• Always AUG• So Met is always the
first amino acid
Stop Codon• DOES NOT code for
an amino acid• Stops translation
2. As ribosome moves, anti-codon tRNA complimentary base pairs to codon.
3. amino acid dropped off. Amino Acids
tRNA
Anticodon
*This is how the DNA instructions are followed & appropriate placement of the amino acids is accomplished creating the correct protein.
4. The process continues until a stop codon is reached.
5. The protein is then released from the ribosome.
To make the process of translation faster and to make many copies of a protein, multiple ribosomes can move along mRNA at
once looking something like this…
Review
DNA: AGCGCTATAAGCTACGCGCAGCATTmRNA: CGAUGGCGUCGUAAtRNA: UACCGCAGC
Protein Met-Arg-Ser
Promoter
Start CodonStop Codon
Rapid Review• Transcription makes mRNA based on the DNA–Occurs in nucleus–Begins at promoter
• Translation assembles protein based on mRNA code–With help of tRNA in ribosome–Begins at start codon. Ends at stop codon
• The structure and shape of the protein depends on the DNA sequence
Keep in mind…• Genes don’t code for
traits• They code for proteins
(or parts of proteins) that can affect traits
• i.e. they can code for a protein that makes eyes blue
• Differences in DNA determine differences in proteins = differences in physical traits.
Transcription Animation
• http://www.hhmi.org/biointeractive/dna/DNAi_transcription_vo2.html
Basic Translation Animation
• http://www.youtube.com/watch?v=1PSwhTGFMxs• http://www.hhmi.org/biointeractive/dna/DNAi_tra
nslation_vo1.html
Detailed Translation Animation
• http://www.hhmi.org/biointeractive/dna/DNAi_translation_vo2.html