Giansimone PerrinoTutor: Diego di Bernardo – co-Tutor: Mario di Bernardo

XXIX Cycle - I year presentation

Identification and Control of Gene Regulatory Networks

2

Background

Giansimone Perrino

Bachelor of Science in Computer Engineering – Università degli Studi di Napoli Federico II

Master of Science in Automation Engineering – Università degli Studi di Napoli Federico II

PhD Student in “Systems and Synthetic Biology Lab” of Diego di Bernardo at Telethon Institute of Genetics and Medicine (TIGEM)• Fellowship provided by TIGEM

3

Problem Statement

Giansimone Perrino

Idea: to use Control Engineering to regulate gene expression in eukaryotic cells

4

GAL1 promoter in Saccharomyces Cerevisiae

Giansimone Perrino

5

Controller Results

Giansimone Perrino

6

Products

Giansimone Perrino

One paper in submission:

i. A comparative analysis of strategies for in-vivo real-time control of protein expression from endogenous and synthetic gene networksGianfranco Fiore, Giansimone Perrino, Mario di Bernardo, Diego di BernardoACS Synthetic Biology In Preparation

7

Next years

Giansimone Perrino

Stochastic modeling and control of biological processes

8

…

Giansimone Perrino

9

GAL1p control: in-silico experiments

Giansimone Perrino

10

GAL1p control: in-vivo experiments

Giansimone Perrino

11

Why?

Giansimone Perrino

To be able to quantitavely assess the behaviour of living systems using an engineering approach.

I.e. which concentration of a-synuclein is needed for aggregate formation in vivo? Right now in molecular biology, genes can only be induced to be ON or OFF but no fine regulation is possible.