Group II

Aleksandra Tkacz UST

Lidia Proczek UST

Katarzyna Michalów UST

Jana Hruba’ STU

Eva Kuzielova’ STU

Krzysztof Burek UST

‘‘Nanoparticles and Nanoparticles and biochemistry’biochemistry’

1. Nanomaterials and quantum dots (QDs)

2. Biochemistry

3. Gd2O3:Tb3+@SiOx system

4. Researches

5. Summary

OutlineOutline

NanoparticlesNanoparticles

Quantum Dots (QDs)Quantum Dots (QDs)Nanometre-scale semiconductor

crystals

Quantum Dots (QDs)Quantum Dots (QDs)How it works ...

Quantum Dots (QDs)Quantum Dots (QDs)Relationship between crystal size and

colour

The smaller the size, the shorter the wavelenght

Quantum Dots (QDs)Quantum Dots (QDs)Advantages in comparison with conventional

Dyes

- QDs emit light at a variety of precise wavelenghts

- QDs can be excited by any wavelenght shorter than the one it emits

- QDs have wide range of avaible colours

- QDs are extremely stable

Quantum Dots (QDs)Quantum Dots (QDs)Ways of obtaining nanoparticles

- Sol-gel

- Plasma

- Mechanochemical

- Aerosol processing

- High-energy ball milling

- Polyol and other chemical

- Hydrothermal processing

- Other chemical and physical techniques as needed

Quantum Dots (QDs)Quantum Dots (QDs)Applications

Life scienceLife science

Controling the size and phase of semiconductor nanocrystals by biological materials (peptides, etc.)

QDs as a markers

GdGd22OO33:Tb:Tb3+3+@SiO@SiOxx [1][1]

[1] Chem. Mater. 2005, 17, 1673-1682

Double Luminescence for Double Luminescence for Biological LabelingBiological Labeling

Optical properties of GdOptical properties of Gd22OO33:Tb:Tb33

Double Luminescence for Double Luminescence for Biological LabelingBiological Labeling

- high photostability

- narrow emission bands

GdGd22OO33:Tb:Tb3+3+@SiO@SiOx x systemsystem

The role of the layer : The role of the layer :

Double Luminescence for Double Luminescence for Biological LabelingBiological Labeling

- allows the dispersion of the particles in aqueous solution without loss of luminescence intensivity

- the luminescence of polisiloxane-coated Gd2O3 nanoparticles is more intense than that in the case of the naked core Gd2O3

- in the presence of amino groups, organic dyes and biotargeting groups (nucleic acid, biotin, streptavid) were covalently linked to the polisiloxane network

Research workResearch work‘Preparation and characterization

of ZnS:Mn nanoparticles’

MaterialsMaterials

Substance Company Purity

Zinc Acetate SIGMA - ALDRICH 98%

Mangan Acetate

ROTH 98% ;)

Sodium Sulphide

RIEDEL-DE-HEAN 60 – 62 %

Cysteamin MERCK 97 %

Ethanol - Technical

Acetic Acid - Technical

Preparation route IPreparation route I

Preparation route IIPreparation route II

ResultsResults

0

10

20

30

40

1 10 100 1000

Vol

ume

(%)

Diameter (nm)

Statistics Graph (2 measurements)

Mean w ith Max-Min error bar

0

5

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15

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1 10 100 1000

Vol

ume

(%)

Diameter (nm)

Statistics Graph (2 measurements)

Mean w ith Max-Min error bar

a)

b)

pH=4,82

pH=4,91

ResultsResults

0

10

20

30

40

1 10 100 1000 10000

Vol

ume

(%)

Diameter (nm)

Size Distribution by Volume

Record 3: ZnS:Mn grupa 2-pH 4.91 Record 4: ZnS:Mn grupa 2-pH 4.91

0

5

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1 10 100 1000 10000

Vol

ume

(%)

Diameter (nm)

Size Distribution by Volume

Record 77: ZnS:Mn grupa 4 -pH 4.82-2ga probaRecord 78: ZnS:Mn grupa 4 -pH 4.82-2ga proba

a)

b)

pH=4,82

pH=4,91

ResultsResults

a)

b)

0

10

20

30

40

1 10 100 1000 10000

Inte

nsity

(%

)

Diameter (nm)

Size Distribution by Intensity

Record 3: ZnS:Mn grupa 2-pH 4.91 Record 4: ZnS:Mn grupa 2-pH 4.91

0

5

10

15

20

1 10 100 1000 10000

Inte

nsity

(%

)

Diameter (nm)

Size Distribution by Intensity

Record 77: ZnS:Mn grupa 4 -pH 4.82-2ga proba Record 78: ZnS:Mn grupa 4 -pH 4.82-2ga proba

pH=4,82

pH=4,91

SummarySummary

- A wide range of future application

- QDs have better properties than conventional Dyes

- The luminescence of QDs @ SiOx systems is very intense

- It’s not difficult to obtain

luminescent nanoparticles

ReferencesReferences

1) C. Louis, R. Bazzi, C.A. Marquette, J-L. Bridot, S.Roux, G. Ledoux, B. Mercier, L. Blum, P. Perriat, O. Tillement Chem. Mater 2005, 17, 1673-1682

2) E. Klarreich Nature 2001, 413, 450-452

3) N.G. Portney, K. Singh, S. Chaudhary, G. Destito, A.Schneemann, M.Machester, M. Ozkan Langmuir 2005, 21, 2098-2103

4) C.E. Flynn, C. Mao, A. Hayhurst, J.L. Williams, G. Georgiu, B. Iverson, A.M. Belcher J.Mater. Chem. 2005, 13, 2414-2421