High Performance Fiber Optic Interface Accessory User's Guide

HIGH PERFORMANCE FIBER OPTIC INTERFACE ACCESSORY

User’s Guide

MOLECULAR SPECTROSCOPY

Release History

Part Number Release Publication Date

L1050018 A

October 2012

Any comments about the documentation for this product should be addressed to: User Assistance PerkinElmer Ltd Chalfont Road Seer Green Beaconsfield Bucks HP9 2FX United Kingdom Or emailed to: [email protected]

The information contained in this document is subject to change without notice. Notices

PerkinElmer shall not be liable for errors contained herein for incidental consequential damages in connection with furnishing, performance or use of this material.

Except as specifically set forth in its terms and conditions of sale, PerkinElmer makes no warranty of any kind with regard to this document, including, but not limited to, the implied warranties of merchantability and fitness for a particular purpose.

This document contains proprietary information that is protected by copyright. Copyright Information

All rights are reserved. No part of this publication may be reproduced in any form whatsoever or translated into any language without the prior, written permission of PerkinElmer, Inc. Copyright © 2012

PerkinElmer, Inc.

Produced in the UK

.

Registered names, trademarks, etc. used in this document, even when not specifically marked as such, are protected by law.

Trademarks

PerkinElmer is a registered trademark of PerkinElmer, Inc. Frontier and Spectrum are trademarks of PerkinElmer, Inc.

. 3

Contents Introduction ............................................................................................... 5 About this User’s Guide ..................................................................................... 6 Conventions Used in this Manual ........................................................................ 7

Notes, cautions and warnings ...................................................................... 7 Safety Practices ....................................................................................... 11 Overview .........................................................................................................12 Precautions ......................................................................................................13 General Operating Conditions............................................................................14 Electromagnetic Compatibility (EMC) Compliance ...............................................15 Installation .............................................................................................. 17 Planning the Installation ...................................................................................18

General .....................................................................................................18 Unpacking .................................................................................................18 Shipping List .............................................................................................18

Installing the Accessory ....................................................................................19 Installing a Fiber Optic Probe ............................................................................21 The Install Accessory Wizard.............................................................................24 Operation ................................................................................................. 27 System Description ...........................................................................................28 Using the Accessory with Spectrum (version 10 or later) .....................................29

Performing a scan......................................................................................30

4 . High Performance Fiber Optic Interface Accessory User's Guide

Introduction


About this User’s Guide

The High Performance Fiber Optic Interface Accessory is designed for use with a range of fiber optic probe accessories. It fits into the sample compartment of Frontier FT-NIR, Frontier FT-IR/FT-NIR, Spectrum 100N FT-NIR, Spectrum 400 FT-IR/FT-NIR, and Spectrum One NTS spectrometers. When fitted with a suitable probe, the accessory allows spectra of materials such as bulk powders to be collected remotely from the spectrometer.

This guide is divided into the following chapters:

Introduction

This chapter describes the conventions and warnings used in this guide.

Safety Practices

This chapter provides important general safety information.

Installation

This chapter describes fitting the accessory hardware, and installing the accessory in the software application.

Operation

This chapter describes the accessory and its use with Spectrum (version 10 or later).

Introduction . 7

Conventions Used in this Manual

Normal text is used to provide information and instructions.

Bold

UPPERCASE text, for example ENTER or ALT, refers to keys on the PC keyboard. '+' is used to show that you have to press two keys at the same time, for example, ALT+F.

text refers to text that is displayed on the screen.

All eight-digit numbers are PerkinElmer part numbers unless stated otherwise.

Notes, cautions and warnings

Three terms, in the following standard formats, are also used to highlight special circumstances and warnings.

NOTE: A note indicates additional, significant information that is provided with some procedures.


CAUTION

We use the term CAUTION to inform you about situations that could result in serious damage to the instrument or other equipment. Details about these circumstances are in a box like this one.

Caution (Achtung) Bedeutet, daß die genannte Anleitung genau befolgt werden muß, um einen Geräteschaden

zu vermeiden.

Caution (Bemærk) Dette betyder, at den nævnte vejledning skal overholdes nøje for at undgå en beskadigelse af apparatet

.

Caution (Advertencia) Utilizamos el término CAUTION (ADVERTENCIA) para advertir sobre situaciones que pueden provocar averías graves en este equipo

o en otros. En recuadros éste se proporciona información sobre este tipo de circunstancias.

Caution (Attention) Nous utilisons le terme CAUTION (ATTENTION) pour signaler les situations susceptibles de provoquer de graves détériorations de l'instrument

ou d'autre matériel. Les détails sur ces circonstances figurent dans un encadré semblable à celui-ci.

Caution (Attenzione) Con il termine CAUTION (ATTENZIONE) vengono segnalate situazioni che potrebbero arrecare gravi danni allo strumento

o ad altra apparecchiatura. Troverete informazioni su tali circostanze in un riquadro come questo.

Caution (Opgelet) Betekent dat de genoemde handleiding nauwkeurig moet worden opgevolgd, om beschadiging van het instrument

te voorkomen.

Caution (Atenção) Significa que a instrução referida tem de ser respeitada para evitar a danificação do aparelho.

Introduction . 9

WARNING

We use the term WARNING to inform you about situations that could result in personal injury

to yourself or other persons. Details about these circumstances are in a box like this one.

Warning (Warnung) Bedeutet, daß es bei Nichtbeachten der genannten Anweisung zu einer Verletzung

des Benutzers kommen kann.

Warning (Advarsel) Betyder, at brugeren kan blive kvæstet

, hvis anvisningen ikke overholdes.

Warning (Peligro) Utilizamos el término WARNING (PELIGRO) para informarle sobre situaciones que pueden provocar daños personales

a usted o a otras personas. En los recuadros como éste se proporciona información sobre este tipo de circunstancias.

Warning (Danger) Nous utilisons la formule WARNING (DANGER) pour avertir des situations pouvant occasionner des dommages corporels

à l'utilisateur ou à d'autres personnes. Les détails sur ces circonstances sont données dans un encadré semblable à celui-ci.

Warning (Pericolo) Con il termine WARNING (PERICOLO) vengono segnalate situazioni che potrebbero provocare incidenti alle persone

. Troverete informazioni su tali circostanze in un riquadro come questo.

Warning (Waarschuwing) Betekent dat, wanneer de genoemde aanwijzing niet in acht wordt genomen, dit kan leiden tot verwondingen

van de gebruiker.

Warning (Aviso) Significa que a não observância da instrução referida poderá causar um ferimento ao usuário.


Safety Practices


Overview

This chapter describes the general safety practices and precautions that must be observed when operating the High Performance Fiber Optic Interface Accessory.

This advice is intended to supplement, not supersede, the normal safety codes in the user's country. The information provided does not cover every safety procedure that should be practiced. Ultimately, maintenance of a safe laboratory environment is the responsibility of the analyst and the analyst's organization.

Please consult all manuals and CDs supplied with the High Performance Fiber Optic Interface Accessory, and your spectrometer, before you start working. Carefully read the safety information in this chapter and in the other manuals supplied. When setting up the spectrometer and accessory, or performing analyses or maintenance procedures, strictly follow the instructions provided.

Figure 1 High Performance Fiber Optic Interface Accessory

Safety Practices . 13

Precautions

WARNING

Be sure that all operators read and understand the precautions listed below. It is advisable to post a copy of the precautions near the instrument.

• Never stare directly into the light projected from the probe, or point this light at another person.

• Do not use the accessory if the spectrometer or accessory shows visible damage.

• If repair or servicing is needed, call your PerkinElmer Service Representative for advice.


General Operating Conditions

The High Performance Fiber Optic Interface Accessory has been designed and tested in accordance with PerkinElmer specifications and in accordance with the safety requirements of the International Electrotechnical Commission (IEC). The High Performance Fiber Optic Interface Accessory conforms to IEC61010-1 (Safety Requirements for electrical equipment for measurement, control and laboratory use) as part of a Frontier FT-NIR, Frontier FT-IR/FT-NIR, Spectrum 100N FT-NIR, Spectrum 400 FT-IR/FT-NIR, and Spectrum One NTS system, and therefore meets the requirements of EC directive 2006/95/EC.

Only use the High Performance Fiber Optic Interface Accessory indoors and under the following conditions:

Temperature 15 °C to 35 °C

Relative Humidity 80% maximum (non-condensing)

Do not use the High Performance Fiber Optic Interface Accessory if it:

• shows visible damage;

• has been subjected to prolonged storage in unfavorable conditions;

• has been subjected to severe transport stresses.

If possible, avoid any adjustment, maintenance or repair of the opened, operating spectrometer. If any adjustment, maintenance or repair of the opened spectrometer is necessary, this must be done by a skilled person who is aware of the hazard involved.

Safety Practices . 15

Electromagnetic Compatibility (EMC) Compliance

European Commission (EC) directive

The High Performance Fiber Optic Interface Accessory has been designed and tested as part of a Frontier FT-NIR, Frontier FT-IR/FT-NIR, Spectrum 100N FT-NIR, Spectrum 400 FT-IR/FT-NIR, and Spectrum One NTS system, to meet the requirements of the EC directive 2004/108/EC. The High Performance Fiber Optic Interface Accessory complies with the EMC standards EN61326 (EMC standard for electrical equipment for measurement, control and laboratory use) and EN55011 (ISM) class A (RF emissions).

Federal Communication Commission (FCC) rules and regulations

This product is classified as a digital device used exclusively as industrial, commercial, or medical test equipment. It is exempt from the technical standards specified in Part 15 of the FCC Rules and Regulations based on Section 15.103 (c).


Installation


Planning the Installation

The information provided in this section is not exhaustive and is for guidance only.

General • Approximately 100 mm (4 inches) of additional bench space to the right of the

spectrometer is needed to accommodate the fiber optic probe accessory (see the appropriate user’s guide for further details).

• To allow the probe cable to hang correctly, the front of the spectrometer must be within approximately 40 mm (1.5 inches) of the edge of the bench.

• The cable clamp on the High Performance Fiber Optic Interface Accessory projects from the edge of the bench; it may be protected by a cushioning flexible molding.

• Make sure that the probe cable does not obstruct a drawer or cupboard under the bench, and that the cable is not a tripping hazard.

Unpacking

The High Performance Fiber Optic Interface Accessory is packed separately from items that are specific to a particular spectrometer, such as the replacement sample compartment cover.

Shipping List

Description Notes Part Number

Replacement sample compartment cover kit for Frontier FT-NIR, Frontier FT-IR/FT-NIR

Includes: Replacement cover Flexible clamp cover molding 1

L1280220 L1251013

Replacement sample compartment cover kit for Spectrum 100N FT-NIR, Spectrum 400 FT-IR/FT-NIR

Includes: Replacement cover Flexible clamp cover molding 1

L1250224 L1251013

Replacement sample compartment cover kit for Spectrum One NTS

Includes: Replacement cover Flexible clamp cover molding

1

L1250225 L1250226

High Performance Fiber Optic Interface Accessory

L1250044

High Performance Fiber Optic Interface Accessory User’s Guide

On the Spectrum Manuals CD L1050002

1 The parts shipped depend on the spectrometer.

Installation . 19

Installing the Accessory

1. Raise the sample compartment cover (if fitted) to the vertical position, press the release clip under the center of its hinge, and then lift the cover upwards, clear of the spectrometer (Figure 2). Store this sample compartment cover in a safe place.

Figure 2 Removing the sample compartment cover

2. Reach under the baseplate of the current accessory, pull the release handle towards you, and then slide the accessory forward out of the sample compartment (Figure 3). Store the accessory in a safe place.

Figure 3 Removing a sampling accessory

Sample cover release clip

Release handle


3. On the left side of the High Performance Fiber Optic Interface Accessory, gently lever off the black optical port cover (Figure 4). Keep this cover in a safe place.

Figure 4 High Performance Fiber Optic Interface Accessory port cover

4. Place the rear edge of the interface on the ledge in the sample compartment and then slide it into the sample compartment until it latches.

5. Tighten the thumbscrew on the left side of the baseplate (Figure 5). This ensures that the High Performance Fiber Optic Interface Accessory is level and cannot move.

NOTE: This step is not applicable to a Spectrum One NTS.

Figure 5 Installing the High Performance Fiber Optic Interface Accessory

After the interface has latched, tighten this thumbscrew (not Spectrum One NTS)

Installation . 21

Installing a Fiber Optic Probe

This section gives some general information about installing a fiber optic probe accessory into the High Performance Fiber Optic Interface Accessory.

1. Carefully route the probe cable to the spectrometer and High Performance Fiber Optic Interface Accessory. The probe cable must be terminated by two SMA optical connectors. These connectors fit into corresponding sockets on the front face of the interface accessory. The cable is clamped to the interface, and the cable clamp covered by a flexible molding.

CAUTION

To prevent damage, avoid bending the probe cable.

2. Support the cable using the clamping plates provided on the interface bracket to prevent strain on the optical connections to the accessory (Figure 6). Tighten the clamps using the two screws until they just support the cables.

Figure 6 Clamping plates to support the fiber optic cables


3. Connect the two SMA optical connectors (Figure 7). Make sure the SMA optical connectors are pushed fully into the interface, and that the securing nuts are fully tightened to prevent movement of the connector. However, do not over-tighten the securing nuts.

Figure 7 Connecting the probe cable to the accessory

4. If required, fit the flexible molding over the cable connector using the screws provided (Figure 8). Do not over-tighten these screws.

Figure 8 Fitting the protective molding

CAUTION

Optical connectors must be clean, so do not remove their protective caps until necessary.

Take care not to touch or scratch the ends of the fiber cables when connecting them.

If you must clean an optical connector, refer to the instructions for the probe accessory you are using.

Cable clamping plates

Flexible molding

Screw

Screw

Interface bracket

SMA optical connector SMA optical connector

Installation . 23

NOTE: The flexible molding supplied depends upon the spectrometer. These moldings are not interchangeable.

5. Fit the replacement sample compartment cover and then close it. The replacement sample compartment cover can be secured using two screws (Figure 9).

NOTE: The securing screws cannot be used with a Spectrum One NTS.

Figure 9 Securing screws for the replacement sample compartment cover

Your High Performance Fiber Optic Interface Accessory is now physically fitted. If you have Spectrum version 10 software, your installation is complete. If you have AssureID version 4.0 (or later) or Spectrum version 6.x software, complete the installation using the Install Accessory Wizard in your software application, as described in the following section.

Screw

Screw


The Install Accessory Wizard

NOTE: This section is not applicable for Spectrum version 10 or later. Spectrum 10 does not automatically perform tests on accessories during installation. For more information on testing accessories, contact your PerkinElmer Service Representative.

NOTE: The accessory installation wizard is common to the Assure ID version 4 (or later) and Spectrum version 6.x software applications. However, the test protocols included in the wizard are only applicable when a Triggered Fiber Optic Probe (L1250055/6/7/9) is attached to the High Performance Fiber Optic Interface Accessory. If you are using a different probe then you will need to perform your own testing of the accessory.

Before you install the High Performance Fiber Optic Interface Accessory you must add and configure an instrument. Refer to the user’s guide for your spectrometer for further details.

Once the instrument is configured, follow the steps below to install your accessory:

1. Login to AssureID Method Explorer or Spectrum (version 6). In the Enhanced Security (ES) version of AssureID you need to login as a Developer; in the Standard version of AssureID you need to login as an Analyst. You need to login to Spectrum version 6 as an Administrator.

2. In AssureID, select Tools, then Configure Instruments and Accessories, and then Configure AccessoriesIn Spectrum, select Administration, then Instrument and Accessory Configuration, and then Add Accessories.

.

The Instrument Install Wizard starts.

Installation . 25

3. Click NextThe Insert Accessory dialog is displayed.

.

If you have not already done so, install the High Performance Fiber Optic Interface Accessory as described in Installing the Accessory on page 19.

The accessory is recognized by the wizard.

4. Click ContinueThe wizard may attempt to perform certain tests; these may not be applicable to the probe you have attached to the accessory.

.

When the accessory tests and calibration have finished, the wizard displays Completed.


5. Click View LogA new window allows you to see the results in more detail.

.

If you want to view the results of the accessories tests later, the log is stored at C:\Program Files\PerkinElmer\ServiceIR\<Instrument Serial Number>\PerformanceTests xxx\PerformanceTests.Log (where xxx is an incremented number). If you are using a 64-bit Windows operating system, the log is stored at the equivalent location in C:\Program Files (x86).

6. Click NextThe Finish page is displayed.

.

7. Click FinishThe accessory is now ready for use.

to close the Instrument Install Wizard.

Operation


System Description

The High Performance Fiber Optic Interface Accessory directs near-infrared (NIR) light from the spectrometer to the tip of the probe attached to the accessory via the fiber optic cables. The probe returns light collected from the sample to the detector in the accessory.

Operation . 29

Using the Accessory with Spectrum (version 10 or later)

When the High Performance Fiber Optic Interface Accessory is installed in the spectrometer,

the software detects the presence of the accessory, and the icon is added to the toolbar.

The Setup Instrument Basic (Figure 10) and Setup Instrument BeamPath (Figure 11) tabs are updated to show that the accessory is in position.

Figure 10 Setup Instrument Basic tab with High Performance Fiber Optic Interface Accessory controls

Figure 11 Setup Instrument BeamPath tab with High Performance Fiber Optic Interface Accessory icon


Performing a scan

1. Enter the required scan and instrument parameters in the Instrument Settings toolbar. For most applications, a spectral resolution of 16 cm−1

When your accessory is installed in the instrument, Spectrum will default to the instrument settings last used to perform a successful scan with that accessory.

and about 30 accumulations should generate high quality spectra.

2. Ensure that Channel 2

Channel 2 is the default setting.

is selected in the Detector Channel drop-down list on the Setup Instrument Basic tab.

3. Select the appropriate GainThe options in the drop-down list are

setting. High or Low

4. Select

. High is the default setting. The Low setting corresponds to a lower energy setting suitable for preventing saturation of transmission probes.

Sample Table

OR

from the Measurement menu.

Open the Data Explorer pane, and then select Sample Table

5. Enter the number of samples and then complete the

.

SampleID and Description

See the on-screen help for more information about adding samples to the Sample Table.

for each sample.

6. If a new background scan is required, you are reminded to make sure that the probe tip is in contact with a Spectralon reference sample. Spectralon is the background reference sample for reflectance probes. If you are using a transmission probe, you will need to use a suitable reference sample for your experiment. Spectrum automatically alerts you when you need a new background, and can be configured to request a new background at set intervals (on the Setup Instrument Data Collection tab).

7. Click BackgroundSpectrum will display a progress indicator to show the background being acquired.

.

8. Once the background is acquired, rest the tip of the probe on your sample and click ScanMaintain the probe tip at a constant position and pressure until scanning is complete. When scanning is complete, the spectrum is added to the Samples View. A green tick in the Sample Table indicates that a sample has been run.

.

If you want to set additional instrument parameters that are not displayed in the Scan toolbars, use the Setup Instrument tabs in the Dialog pane at the bottom of the workspace.

The Spectrum on-screen Help describes how to format, process and report your results. To view the Help, select Contents from the Help menu.

Documents

High Performance Fiber Optic Interface Accessory User's Guide