Http Cheng.dlearn.kmu.Edu.tw Ppt TissueEngineering

8/13/2019 Http Cheng.dlearn.kmu.Edu.tw Ppt TissueEngineering

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Tissue Engineering
http://www.i-s-b.org/fokus/tissue/images/tissues2.jpg


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http://www.youtube.com/watch?v=PEc7QXAjsL4&feature=related

Mouse Ears

1997 MIT
http://www.youtube.com/watch?v=PEc7QXAjsL4&feature=relatedhttp://www.youtube.com/watch?v=PEc7QXAjsL4&feature=relatedhttp://www.manager-magazin.de/img/0,1020,126500,00.jpg


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http://www.youtube.com/watch?v=WvSxZ4JKQAY&feature=related

Engineering Tissue
http://www.youtube.com/watch?v=WvSxZ4JKQAY&feature=relatedhttp://www.youtube.com/watch?v=WvSxZ4JKQAY&feature=related


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Corneal Tissue Engineering

Blood vessel tissue engineering

Liver tissue engineering

Cartilage tissue engineering

Bone tissue engineering


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Cell Culture( stemcell, osteoblast,

chondrocyte,

hepatocyte

Signaling

Molecules(GrowthFactors, proteins

Scaffold( ceramics

collagen, polymers)

Tissue/Organ

regeneration

Key Factors involving tissue engineering


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Cell culture

Interactive traids of responsive cells

Scaffolds

Supportive matrix

Growth factorsBioactive molecules promoting differentiation

and regeneration


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Application of tissue engineering

3D culture

Transplantation

Cellular and tissuebases product

Alternative to animal

test
http://www.aist.go.jp/aist_e/ressearch_units/research_center/terc/terc_01.gifhttp://www.aist.go.jp/aist_e/ressearch_units/research_center/terc/terc_01.gifhttp://www.aist.go.jp/aist_e/ressearch_units/research_center/terc/terc_01.gifhttp://www.aist.go.jp/aist_e/ressearch_units/research_center/terc/terc_01.gifhttp://www.aist.go.jp/aist_e/ressearch_units/research_center/terc/terc_01.gifhttp://www.aist.go.jp/aist_e/ressearch_units/research_center/terc/terc_01.gifhttp://www.aist.go.jp/aist_e/ressearch_units/research_center/terc/terc_01.gifhttp://www.aist.go.jp/aist_e/ressearch_units/research_center/terc/terc_01.gif


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Developing procedures used in cartilage tissue engineering

1st generation 2nd generation 3rd generation

Principle

approachAutologous cell

transplant

Performed tissue

flaps 3D construct

In vivo regeneration

and guided tissue

repair

Importantcomponent

Periostealflaps( )Delivery

substances and

scaffold( )

Growth factors and

biomaterials( )


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Cartilage Tissue Engineering

I. Autologous chondrocyte

1. Isolation of tissue sample from patients

2. Tissue regeneration by precursor cells

or embryonic stem cells

articular cartilage repair, nasal septal

cartilage scaffolds for cartilage for

tissue repair


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ECM ( Extra cellular Matrix)

ECM interaction directly influence cell signaling via

cell adhesion molecules such as integrinand

cadhedrin, fibronectin, collagen, laminin.


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Extracellular Matrix in relation to epithelium endothelium and

connective tissue

Epithelial cell

Basement

membrane

Endothelial lining the

capillary

Connective tissue with

interatitial matrix

Fibroblast


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http://inano.au.dk/organization/research-groups/nanobiointerfaces/research/steering-stem-cell-differentiation/

Steering Stem Cell Differentiation

Differentiation of stem cells induced by binding to extracellular

matrix components(e.g. proteins such as fibronectin, collagen

and laminin)components


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II. Scaffold for tissue construction

1. Act as shapeand guidancetemplate for

in vi t roand in vivotissue development

2. Permit 3D immobilization and maintain

differentiation

materials: hyaluronic acid, collagen, alginate,

chutosan, synthetic biodegradable poly( -

hydroxy ester) polyacetic acid,( PLIA), polyglycolic

acid( PGA), PLGA


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Chondrocyte or

Mesenchyma stem cell

Polymer fiberEmbedding medium

( hydrogel)


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Biosynthetic scaffolds

Collagen scafold
http://staff.bath.ac.uk/cesmlj/2_1c-pbs.gif


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Tissue scafold +Cellsand

Growth factors

In vivotransplant


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http://www.youtube.com/watch?v=0QJh0yc2mAI&feature=related

Engineering Organs from Scratch


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Tissue in cardiovascular structure

Advantages:1. Avoid repeat operation

2. No rejection by the patients immune system

3. Cells behave like endothelial cells may have

low thrombus formation and low infection

i.e. valves, blood vessels, vascular patches


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Basic steps in tissue engineering of a cardiac valve

Bone marrowcells harvest

Valve

scaffold

Cell- scaffold

construct


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A h t t l ft
http://bme.dentistry.dal.ca:16080/bme/mlee/PorcineValve.jpg


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Approaches to create a vascular graft

Cross

linkingExogenous

chemicals

Mechanical

stimulations

Cells + tubular scaffold Endothelial lining In vivo

implantation

Smooth muscle cells

progenitor cells

collagen gel/elastin

fibrin

Extra cellular matrix

Biodegradable

polymers

Autulogous EC

Circulating EPCAllogenic EC

Embryonic stem

cell

Non-thromgenicityImmune response

Remodeling

American Journal of Transplantation 2004; 4 (Suppl. 6): 3642


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http://www.synthecon.com/Graphics/pga_lg.jpg


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http://www.fibercellsystems.com/images/cross-section.jpg


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http://channel.nationalgeographic.com/series/explorer/4828/Photos/3671755b

e79cd210VgnVCM1000000100007fRCRD#tab-Videos/09347_00

How to Build a Beating Heart

http://channel.nationalgeographic.com/series/explorer/4828/Pho

tos/3671755be79cd210VgnVCM1000000100007fRCRD#tab-

Videos/10008_00

http://channel.nationalgeographic.com/series/explorer/4828/Pho

tos/3671755be79cd210VgnVCM1000000100007fRCRD#tab-

Videos/09346_00
http://channel.nationalgeographic.com/series/explorer/4828/Photos/3671755be79cd210VgnVCM1000000100007fRCRDhttp://channel.nationalgeographic.com/series/explorer/4828/Photos/3671755be79cd210VgnVCM1000000100007fRCRDhttp://channel.nationalgeographic.com/series/explorer/4828/Photos/3671755be79cd210VgnVCM1000000100007fRCRDhttp://channel.nationalgeographic.com/series/explorer/4828/Photos/3671755be79cd210VgnVCM1000000100007fRCRDhttp://channel.nationalgeographic.com/series/explorer/4828/Photos/3671755be79cd210VgnVCM1000000100007fRCRDhttp://channel.nationalgeographic.com/series/explorer/4828/Photos/3671755be79cd210VgnVCM1000000100007fRCRDhttp://channel.nationalgeographic.com/series/explorer/4828/Photos/3671755be79cd210VgnVCM1000000100007fRCRDhttp://channel.nationalgeographic.com/series/explorer/4828/Photos/3671755be79cd210VgnVCM1000000100007fRCRDhttp://channel.nationalgeographic.com/series/explorer/4828/Photos/3671755be79cd210VgnVCM1000000100007fRCRDhttp://channel.nationalgeographic.com/series/explorer/4828/Photos/3671755be79cd210VgnVCM1000000100007fRCRDhttp://channel.nationalgeographic.com/series/explorer/4828/Photos/3671755be79cd210VgnVCM1000000100007fRCRD


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Artificial skin

skin substitute

Adhere to the substrate,

Durable and sufficiently elastic

Tolerate some deformation

Allow evaporative water lossProvide a microbial barrier

Promote homeostasis

Easy to use

Readily availableimmediately after injury, and elicit a

Regeneration-like response from the wound bed

without evoking an inflammatory, foreign-body, or non-

self immunologic reaction.


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Annu. Rev. Med. 2000. 51:231244


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Artificial skin
http://www.semibio.com/images/ct1.jpghttp://www.devicelink.com/mddi/archive/98/05/9805d84a.jpg


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Artificial skin


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Therapy using Artificial skin


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MSC (mesenchyma stem cells)

Proliferate in cultures

Differentiate into multiple mesenchyma lineageie. correction of genetic disorder of , i.e bone cartilage


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Cell seeding on cotton gauzeframed collagen substratum

Formation of multilayer

mesenchyma cells

Physical detachment

3D culture

Preparation of 3-D mesenchyma cells



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Seeding of

mesenchyma cells

confluent

mesenchyma cells

Seeding of

epithelial cells

Coculture of epithelial

and mesenchyma cells

Detachment of epithelialcellsattached

mesenchyma cell sheet

Formation of

hetero-spheroid



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Osteoblast

Arise from mesenchyma cells( MSC)

MSC may be differentiate into osteoblast in vitro

Cultured osteoblast may form extracellular

matrix in culture


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Bone marrow derived

osteoblast

Cultured bone

T. Uemura et al. / Biomaterials 24 (2003) 22772286

Transplantation of cultured bone cells


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Bone marrow derivedosteoblast

Cultured bone

Transplantation of cultured bone cells



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http://www.jove.com/details.php?id=2691

Elastomeric PGS Scaffolds in Arterial Tissue Engineering

http://www.youtube.com/watch?v=ofiLcTs7_Ys&feature=related

Tissue Engineering -- Building Body Parts
http://www.jove.com/details.php?id=2691http://www.youtube.com/watch?v=ofiLcTs7_Ys&feature=relatedhttp://www.youtube.com/watch?v=ofiLcTs7_Ys&feature=relatedhttp://www.jove.com/details.php?id=2691

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Http Cheng.dlearn.kmu.Edu.tw Ppt TissueEngineering