IETS - Registration Book

3636thth Annual Conference of the IETS /2323rdrd Annual Meeting SBTE

Sheraton Cordoba HotelJanuary 9-12, 2010Cordoba, Argentina

Program & Registration Book

Invitation from the Presidents

Table of Contents1 ... Invitation from the Presidents2 ... Tentative Calendar of Events

4 ... Registration Information5 ... General Information6 ... Travel Information

7 ... Accommodation Information8 ... Additional Accomodation Information

9 ... Program13 ... Pre-conference Satellite Symposium14 ... Pre-conference Satellite Workshop15 ... Pre-conference Satellite Workshop

16 ... Special Events17 ... Cordoba Information and Attractions

18 ... Job Resouce Center19 ... Membership Application

It is a great pleasure to invite you to the annual meeting of the International Embryo Transfer Society (IETS) in Cordoba, Argentina from January 9th to 12th, 2010. The IETS is a dynamic group of scientists that focuses on all facets of embryo technologies, including embryo transfer, in vitro embryo production, embryo culture, cloning, and transgenesis in species ranging from domestic agricultural, laboratory and companion animals to wild, endangered species and humans.

The 2010 meeting is an exciting new venture for IETS as we embark on our fi rst truly joint meeting with another society - the Brazilian Embryo Technology Society (Sociedade Brasileira de Tecnologia de Embriões, SBTE). The Program Chairs, Matt Wheeler and Gabriel Bo, have put together an exciting program for the main conference entitled ‘Back to basics: from the bench to the fi eld’. Given the range of invited speakers the program promises to have something for the scientist and practitioner alike. In addition, a pre-conference symposium organized by Reuben Mapletoft and our partners, Bioniche Animal Health, will deal with new developments in the practice of embryo transfer while Fulvio Gandolfi will organize a half day workshop on current progress in embryonic stem cell research in domestic animals. Thanks to the hard work of the local organizing team, headed by Gabriel Bo, the meeting promises to be a great success.

Put the dates in your diary now and we look forward to meeting you in Cordoba.

Best wishes

Pat Lonergan, President IETS

Fernando Garcia, President SBTE

36th Annual Conference2

Tentative Calendar of Events

Event times and locations are subject to change; events may be added.Thursday, January 7, 20109:00 - 18:00 IETS Board of Governors Meeting (Sala A)

Friday, January 8, 20109:00 - 18:00 IETS Board of Governors Meeting (Sala A)9:00 - 18:00 Health and Safety Advisory Committee (HASAC) - Research Subcommittee (Suquia A)16:00 - 19:00 Registration (pick up of pre-registrations only) (Main Foyer)

Saturday, January 9, 20107:00 - 18:00 Registration (Main Foyer)9:00 - 12:00 Health and Safety Advisory Committee (HASAC) - Regulatory Subcommittee (Suquia A)8:00 - 18:00 Pre-Conference Symposium: New Developments in the Practice of Embryo Transfer (Lugones A)13:00 - 18:00 Poster Setup (Champaqui and Yacanto)13:00 - 17:00 IETS Foundation Board of Trustees Meeting (Sala A)13:00 - 18:00 Commercial Exhibit Setup (Lugones Foyer)14:00 - 17:00 Health and Safety Advisory Committee (HASAC) - Food Safety Subcommittee (Suquia A)14:30 - 18:00 DABE Pre-Conference Workshop: Current Progress in Domestic Animals Embryonic Stem Cell

Research (Lugones C)

Sunday, January 10, 20107:30 - 8:30 Past President’s Breakfast (Suquia)7:30 - 8:30 Student Competition Breakfast with Foundation Education Committee (VIP - Las Sierras)7:00 - 18:00 Registration (Main Foyer)8:00 - 17:00 Commercial Exhibition (Lugones Foyer)8:00 - 17:00 A/V Library/Speaker Preparation (Uritorco A)8:30 - 9:30 IETS Foundation Education Committee (Sala A)8:45 - 9:00 Opening and Welcome (Lugones)9:00 - 10:30 Session I: The Donor Animal (Lugones)10:30 - 11:00 Refreshment Break/Exhibition (Lugones Foyer)11:00 - 12:30 IETS Foundation Student Competition Presentations (Lugones)12:30 - 14:00 Lunch Break 12:30 - 14:00 IETS Board Luncheon with Affi liate Society Representatives (Suquia A)12:30 - 14:00 Health and Safety Advisory Committee (HASAC) - Forms & Certifi cates Subcommittee

(Suquia B)14:00 - 16:00 Session II: Control of Environmental Conditions in the Lab and Field (Lugones)16:00 - 16:30 Refreshment Break/Exhibition (Lugones Foyer)16:30 - 18:30 Session III: Embryo Manipulation (Lugones)18:30 - 19:30 Health and Safety Advisory Committee (HASAC) Open Meeting (Lugones)20:00 Welcome Reception (Lugones Room and Lugones Foyer)

International Embryo Transfer Society3

Tentative Calendar of Events

Event times and locations are subject to change; events may be added.Monday, January 11, 20107:00-8:00 Exhibitors Breakfast with Board of Governors (Suquia)7:30 - 16:00 Registration (Main Foyer)8:00 - 18:00 Commercial Exhibits (Lugones Foyer)8:00 - 17:00 A/V Library/Speaker Preparation (Uritorco A)9:00 - 10:30 Poster Session I/Refreshment Break/Exhibition (Champaqui and Yacanto / Lugones Foyer)10:30 - 12:30 Session IV: Production of pregnancies (Lugones)12:30 - 14:00 IETS Data Retrieval Committee Meeting (Suquia)12:30 - 14:00 Lunch Break 14:00 - 16:00 Session V: Production of Live Offspring (Lugones)16:00 - 16:30 Refreshment Break/Exhibition (Lugones Foyer)16:30 - 17:00 IETS Pioneer Award Presentation (Lugones)17:00 - 18:00 IETS Annual Business Meeting (Lugones)18:00 - 19:00 Companion Animal, Non-Domestic & Endangered Species (CANDES) Open Meeting

(Lugones)18:00 - 19:00 Domestic Animal Biomedical Embryology Committee (DABE) Open Meeting (Suquia)

Tuesday, January 12, 20107:00 - 8:30 Organizational Meeting of the IETS Board of Governors. (Sala A)8:00 - 15:00 Registration (Main Foyer)8:00 - 13:30 Commercial Exhibits (Lugones Foyer)8:00 - 17:00 A/V Library/Speaker Preparation (Uritorco A)9:00 - 10:30 Poster Session II/Refreshment Break/Exhibition (Champaqui and Yacanto / Lugones Foyer)10:30 -12:30 Practitioners’ Forum: Cattle IVF in South America: A short story of great results… (Lugones)12:30 - 13:30 Lunch break 12:00 - 13:30 Organizational Lunch Meeting of the IETS Foundation (Sala A)13:30 - 17:00 Commercial Exhibit & Poster Teardown (Lugones Foyer, Champaqui and Yacanto)13:30 - 14:00 IETS Distinguished Service Award Presentation (Lugones)14:00 - 14:45 IETS Foundation Student Competition Awards, CANDES & HASAC Updates (Lugones)15:00 - 15:45 Session VI: Keynote Address (Lugones)15:45 - 16:00 Closing Ceremony (Lugones)16:00 - 17:00 SBTE Business Meeting (Lugones)17:15 - 18:15 8th IETS Annual Running Competition (Meet at registration desk)19:00 - 8:00 Closing Party (Meet near registration desk for the buses)

Wednesday, January 13, 20108:30 - 17:00 Workshop for Authors: Publishing Scientifi c Papers in English (day 1)

(Universidad Católica de Córdoba, Obispo Trejo 323, Cordoba)

Thursday, January 14, 20108:30 - 17:00 Workshop for Authors: Publishing Scientifi c Papers in English (day 2)

(Universidad Católica de Córdoba, Obispo Trejo 323, Cordoba)

Check out the IETS Web Site (http://www.iets.org/2010) for changes in the Calendar of Events and other activities as well as links to the sponsor web sites.


Registration MaterialsAll registration materials are included in this mailing. Each technical registrant should complete the enclosed General Registration Form. One registrant per form. Please use the enclosed form to register for all events, including the Workshops and Satellite Symposium. Registration materials are also available on the IETS web site at http://www.iets.org/2010.

Registration DeadlineRegistrations that are postmarked on or before November 11, 2009 will receive a lower rate than those who send in their registrations after November 11, 2009. No telephone or e-mail registrations will be accepted. FAX ((217) 398-4119) registrations will be accepted only if payment is made by credit card. Be sure to pre-register to avoid higher prices, missed events and long lines in Cordoba.

Membership InformationYou do not have to be a member of the IETS to attend the conference. However, the difference between the member and nonmember registration fees makes joining the Society at the time of registration very attractive. A membership application for the IETS is included in this booklet on page 16.

PaymentPayment must accompany the registration form. Checks must be in US funds drawn on a US bank made payable to the IETS. Payment by credit card (Visa, MasterCard, American Express or Discover) is available. Please complete the credit card payment section of the General Registration Form.

Confi rmations/ReceiptsIf you pre-register by November 11, 2009, we will mail you a registration confi rmation/receipt. Please verify the receipt and events registered for and contact the IETS Business Offi ce with any questions.

Calendar of EventsIncluded on pages 2 and 3 of this booklet is tentative calendar of events for the conference. Event times and locations are subject to change and certain events may be added.

ProceedingsParticipants will receive a CD copy of the proceedings with their registration packet in Cordoba; a printed copy of the proceedings can be purchased by fi lling out the appropriate section on the registration form. IETS members who are unable to attend the conference will be mailed a CD copy of the proceedings after the conclusion of the event.

ReminderWear your name tag at all functions; it is your admission pass to all conference events. Tickets for special events and functions will be collected at the door or at the table. All tickets look alike, but event name, location and date will appear on them. Be sure to give the ticket takers the appropriate ticket.

Special NeedsAll conference rooms are wheelchair accessible. Please indicate any special needs when sending in your registration form.

Questions???All inquiries about pre-registration or the conference should be made to the IETS Business Offi ce, 2441 Village Green Place, Champaign, IL 61822 USA; phone: (217) 398-4697; FAX: (217) 398-4119; e-mail: [email protected].

On-Site Registration HoursFriday, January 8 16:00–19:00

Pre-registration Pickup Only—On-site registration to start Saturday

Saturday, January 9 07:00–18:00 Sunday, January 10 07:00–18:00 Monday, January 11 07:30–16:00 Tuesday, January 12 08:00–15:00

Registration Information


General InformationAt the ConferenceYour conference packet will be available at the Registration Desk, located in the Main Foyer, during registration hours.

VenueSheraton Cordoba Hotel, Cordoba, Argentina (http://www.sheraton.com/cordoba)

Lunch & Dining OptionsRESTAURANT LA PAMPA - SHERATON CÓRDOBA HOTELRestaurant La Pampa offers unique alternatives of national and international cuisine, with a wide range of wines, including those from the main national wineries.

With capacity for 120 people, the Restaurant La Pampa is a must. Enjoy the refreshing daily buffet breakfast offering tasty coffees,delicious natural juices, seasonal fruits and freshly prepared homemade traditional bakery.

NUEVO CENTRO SHOPPINGDirectly in front of the Sheraton Hotel, is Nuevo Centro Shopping, with a large Food Court in the lower level. There is also a restaurant on the second fl oor.

There is also a wide range of opportunities nearby the Sheraton Córdoba Hotel and in downtown Córdoba. These options will be provided at the Registration Desk, January 9, 2010

ClimateJanuary is summer in Argentina. During the summer season the days are warm but the nights display a pleasant temperature decrease. The typical Cordoba January average temperatures are 64ºF (18ºC) (low) and 88°F (31ºC) (high).

CurrencyThe Peso is the legal tender in Argentina. There are currency exchange centers located in Cordoba Airport and in tourist agencies and banks in downtown and shopping centers. For up to date currency exchange information check the Universal Currency Converter™ at http://www.xe.net/ucc/. International credit cards are accepted throughout the country.

ElectricityThe electric current in Argentina is 220 volts, 50 cycles. To use equipment designed for 110 volts, it is necessary to use a converter.Flat-pronged plugs, North American and European, need adapters; since the plugs in Argentina are continental, with double round or triple fl at prongs.

LanguageThe offi cial conference language will be English. However, simultaneous translation to Spanish and Portuguese will be provided.

Time ZoneArgentina’s time zone is 3 hours behind GMT (= GMT - 3).

Passport & Visa InformationAll foreigners who wish to enter in Argentina require a passport valid for six months. The natives of Bolivia, Brazil, Chile, Paraguay and Uruguay only require a corresponding identifi cation of national identity.

People wishing to enter Argentina as tourists or on business, do not require visas if they come from the following countries: Andorra, Austria, Australia, Belgium, Bolivia, Brazil, Chile, Colombia, South Korea, Costa Rica, Croatia, Cyprus, Denmark, Ecuador, Spain, El Salvador, Slovenia, Slovakia, Estonia, Finland, France, Germany, Greece, Grenada, Guatemala, Guyana, Haiti, Holland, Honduras, Hong Kong, Hungary, Iceland, Ireland, Israel, Italy, Jamaica, Japan, Latvia, Liechtenstein, Lithuania, Luxembourg, Malaysia, Malta, Mexico, Monaco, Nicaragua, Norway, New Zealand, Panama, Paraguay, Peru, Poland, Portugal, United Kingdom and Northern Ireland, Czech Republic, Dominican Republic, San Marino, Saint Lucia, Serbia and Montenegro, Singapore, Vincent and Grenadines, Sweden, Switzerland, Trinidad and Tobago, Turkey, Uruguay, Vatican and Venezuela. Canadian citizens must indicate that they are entering Argentina as tourists.

For more information please consult the Embassy of Argentina in your country or the web site of Ministry of Foreign Affairs, International Trade and Worship of Argentina: http://www.mrecic.gov.ar/portal/temas_consulares/visas.html


Air TransportationThe International Airport Ambrosio Taravella, also know as Pajas Blancas is located 9 km (5.6 mi) north-northwest of Córdoba. The airport connects Córdoba directly with over 100 cities throughout the world. The airport is served by 24 passenger carries and accommodates approximately 600 arrivals and departures each day. On any given day an average of 50,000 passengers will arrive or depart.

Ground TransportationThe International Airport Ambrosio Taravella, is connected by a modern road infrastructure to downtown. Remis Global is available to transport people from the Airport to their hotels for a set price.

Remis GlobalInternational Airport Ambrosio TaravellaArrival Area - Central Hall Phone: + 54 351 4753083 / 4753030 / 4753020Cel phone: + 54 (9) 351 4038596E-mail: [email protected] Cost: - From the Airport to Sheraton Córdoba Hotel: $55 argentine pesos (USD $14.90). - From the Airport to other hotels in city center: $53 argentine pesos (USD $14.32).

Regular taxis are also available at the front of the main arrival level. Aproximate cost: $30 argentine pesos (USD $ 8).

Reference: Exchange rate is USD $1 = $ 3.8 argentine pesos (September 9, 2009)

Recommended Transportation from the Buenos Aires International Airport (Ezeiza) to the domestic Airport Jorge Newbery (Aeroparque).Manuel Tienda LeónInternacional Airport Ministro Pistarini (Ezeiza)Phone: + 54 11 4315 5115 0 810 888 [email protected]: - From Ezeiza Airport to Aeroparque Airport by bus: $45 argentinian pesos (USD $11.85). - From Ezeiza Airport to Aeroparque Airport by remis: $141 argentinian pesos (USD $37.10).

Travel Information


Accommodation InformationSheraton Cordoba HotelDuarte Quiros 1300 Phone: (54)(351) 526 9000Cordoba, Cordoba 5000 [email protected] www.sheraton.com/cordoba

General Hotel InformationEnjoy a warm welcome at the Sheraton Cordoba Hotel. Ideally located near the heart of downtown, our hotel, the Sheraton Cordoba Hotel, is convenient to many of the city’s main tourist attractions, exclusive shopping areas, and recreational districts. It is also just minutes from Cordoba International Airport. In addition to its boutique style, the striking architectural design of the hotel, with its 16 floors rising gracefully around a central atrium, offers beautiful views of Cordoba’s downtown and immense green ranges. Gather with friends or colleagues and enjoy a wide range of local and international cuisine at La Pampa Restaurant or cocktails and drinks in the Lobby Bar.

Room Rates Available for the IETS 2010 Annual Conference:The Hotel confi rms the following guest room rates:Single Occupancy $135Double Occupancy $135Breakfast is inlcuded with the room.

The 188 tastefully-appointed guest rooms and suites offer complete comfort and modern decor in an elegant atmosphere. Spacious, luminous, and comfortable, rooms afford a beautiful view of the city and wonderful amenities. The Sheraton room accommodations include a variety of in-room amenities: 24-hour room service, non-smoking room, cable channels, iron/ironing board, 29” television with remote control, international direct dialing, in-room electronic safe, hairdryer , wake-up service, alarm clock, smoke detectors in room, voicemail, sprinklers in room, work desk, data port, radio, and individual climate control.

ReservationsPlease make your reservations directly with the hotel.Make sure to indicate that you are with the International Embryo Transfer Society Meeting.To make reservations by phone please call (54)(351) 526 9000.

To make reservations via email, please send the following information to [email protected]:NameAddressPhoneEmail (very important, confi rmation will be sent via email)Arrival DateDeparture DateRoom Type - (Single, Double with 2 twin beds or 1 large bed, etc.)Specials - Smoking/Non Smoking - Upper/lower fl oors - etcGuarantee - Credit Card Number, Expiration Date, Security Code


Additional Accommodation Information

Hotel ReservationsThe deadline for hotel reservations is December 12, 2009.

Please make your hotel reservation before this date to ensure that you receive the discounted rate.

Amerian (****)US$ 121Bv. San Juan 165 (X5000ATB) Córdoba, ArgentinaTelephone: +54 351 420 [email protected]

NH-Panorama (****)Single: U$S 97 Double: U$S 105Marcelo T. de Alvear (La Cañada), 251. X5000KGE Cordoba, ArgentinaTelephone: +54.35.14103900 [email protected] www.nh-hotels.com/nh/en/hotels/argentina/cordoba/nh-panorama.html

NH-Urbano (***)Single: U$S 84 Double: U$S 84Marcelo T.de Alvear(La Cañada), 363. X5000KGF Cordoba, ArgentinaTelephone: +54.35.14103960 [email protected] www.nh-hotels.com/nh/en/hotels/argentina/cordoba/nh-urbano.html

King David (****)US$ 94Av. General Paz 386 - X5000JLQ Córdoba, ArgentinaTelephone: [email protected]

Holiday Inn (*****)Standard: U$S 133 Executive: U$S 157 Suite: U$S 242Fray Luis Beltrán y M. Cardeñosa Bº Poeta Lugones Cordoba, ArgentinaTelephone: +54 (0351) 4779156/[email protected]


ProgramMain Theme: Back to the Basics, From the Bench to the Field

Session I: The Donor AnimalRecent Progress in Folliculogenesis: Coordinated Regulation by Germ and Somatic Cells Bruce D. Murphy, University of Montreal, St-Hyacinthe, QC, Canada The continuum of folliculogenesis begins in the fetal ovary with the differentiation of the oogonia and their isolation

within the primordial follicles. Primordial follicle activation is an enigmatic process, whereby some follicles enter the growing pool to become primary follicles, thereby embarking on an irreversible progression toward ovulation or atresia. This process is under the coordinated regulation of factors from the oocyte itself, and from the somatic cells of the ovary, in particular, the theca and granulosa cells that are structural components of the follicle. These two infl uences provide the principal stimuli for the growth of the follicle to the late preantral or early antral stage of development. The endocrine infl uences of the gonadotropins, FSH and LH are essential to the continued progression of the follicle, and most atresia can be attributed to the failure to receive or process the gonadotropin signals. The peri-ovulatory state has received intensive investigation recently, demonstrating a coordinated role for gonadotropins, steroids, epidermal growth factor family proteins and prostaglandins. Thus, a complex program of coordinated interaction of governing elements from both germ and somatic cell sources is required for successful follicle development.

Mammalian Oocyte Development; Checkpoints for Competence Trudee Fair, University College Dublin, Ireland

During the lifetime of female mammals, husbandry practices, production demands and disease induce biochemical changes in the ovarian environment. These changes can have a profound effect on ovarian oocyte quality and subsequently embryo development. Although it is widely accepted that poor oocyte quality can be a contributing factor to infertility in cattle, there is no consensus regarding the time or period of follicular oocyte development that is particularly sensitive to insult. Here the key molecular and morphological events that occur during mammalian oocyte and follicle growth are reviewed, with a specifi c focus on identifying critical checkpoints in oocyte develop-ment. Two stages of oocyte development emerge as potential checkpoints: The secondary follicle stage, as major events such as the activation of the oocyte transcriptome and sequestration of oocyte specifi c organelles occur and several months later, the periovulatory period, when essential mRNA transcript processing occurs, meiosis resumes and chromosomes must segregate and align correctly. A comprehensive review of the literature pertaining to the identifi cation of biomarkers of oocyte quality and competence is presented with particular emphasis on data available for bovine oocytes.

Session II: Control of Environmental Conditions in the Lab and FieldIs the Zona Pellucida an Effi cient Barrier for Viral Infection?Ann van Soom, Ghent University, Merelbeke, Belgium

Although transfer of bovine embryos is much less likely to result in disease transmission than transport of live animals, the sanitary risk associated with bovine embryo transfer remains the subject of both scientifi c investigations and adaptations of national and international legislation: these directives have therefore important consequences for the veterinary practitioner. Besides in vivo derived cattle embryos, also in vitro produced embryos or embryos from other species such as pigs and horses are being transferred in fairly signifi cant numbers nowadays and this fact may call for new studies in this important area of research, which provides a link between science and practice. With the wide variety of embryos and the large number of corresponding viruses in mind, it is convenient to reconsider in this review the importance of the zona pellucida as a barrier for viral infection. For embryos with an intact zona, viral infection of the embryo is unlikely to occur. The virus may however stick to the zona pellucida and in this case, IETS washing procedures in combination with trypsin treatment are mandatory. Caveats are the fact that more and more types of embryos become available for transfer nowadays, and scientifi c data cannot be extrapolated from one species to another.


ProgramTowards Use of Microfl uidics for Individual Embryo Culture Rebecca Krisher, University of Illinois, Urbana, IL, USA

Mammalian embryo development is still relatively ineffi cient in vitro. Much research has been conducted on the chemical environment, or culture medium, surrounding the embryo, but little attention has been given to the actual physical culture environment, which has changed very little over the years. The application of microfl uidics to embryo production in vitro is a tantalizing approach that may alleviate some of the limits that traditional microdrop culture places on embryo development, and the research of gamete and embryo physiology. These devices may lead to enhanced in vitro embryo development and quality in vitro, by more closely mimicking the in vivo environment. Initial work in this area is promising, and gives us proof of principle that these unique microfl uidic systems may indeed be applicable to in vitro culture of gametes and embryos. This manuscript will review the advantages of microfl uidics for in vitro embryo production, briefl y how the platforms are manufactured, the current uses of microfl uidics in assisted reproduction, static versus dynamic culture environments, individual gamete and embryo culture, and the future directions of microfl uidic application to in vitro embryo production and manipulation. Finally, preliminary data in our laboratory using a new microfl uidic well insert for porcine, bovine and murine embryo culture will be discussed.

Session III: Embryo ManipulationChallenge Testing of Gametes to Enhance Their ViabilityHenrik Callesen, Danish Institute of Ag. Sciences, Tjele, Denmark

Embryos, oocytes and spermatozoa undergo several manipulations during the in-vitro procedures that are an integrated part of the various assisted reproductive technologies (ART) in mammals. As one consequence of this, some of the gametes are damaged irreparably, while others have the ability to react with a survival mechanism that brings them through the challenging conditions - stronger and more viable. The details of such a survival mechanism remain unknown, but if identifi ed, it could have immense potential as a new way to improve viability of embryos produced by ART. However, only few publications describe systematic ways to challenge test the gametes with use of the result as a basis for improving gamete viability. Furthermore, new methods for monitoring the reaction(s) of gametes to such challenge tests are needed. In this review, these two issues are discussed, as well as some of the conditions that are required for a challenge test protocol to be part of the future work with ART.

Applications of RNA Interference Based Gene Silencing in Animal AgricultureCharles Long, Texas A & M University, College Station, TX, USA

Classical genetic selection, recently aided by genomic selection tools, has been successful in achieving remarkable progress in livestock improvement. However, genetic selection has led to decreased genetic diversity and in some cases acquisition of undesirable traits. In order to meet the increased demands of our expanding population new technologies and practices must be developed that contend with zoonotic and animal disease, environmental impacts of large farming operations and the increased food and fi ber production needed to feed and cloth our society. Future increases in productivity may be dependent upon the acquisition of genetic traits not currently encoded by the genomes of animals used in standard agricultural practice, thus making classical genetic selection impossible.

Genetic engineering of livestock is commonly used to produce pharmaceuticals or to impart enhanced production characteristics to animals, but has also demonstrated its utility in producing animals with disease resistance. However, signifi cant challenges remain, as it has been more diffi cult to produce animals in which specifi c genes have been removed. It is now possible to modify livestock genomes to block expression of endogenous and exogenous genes (such as those expressed following virus infection). In this review we discuss mechanisms of silencing gene expression via the biology of RNA interference (RNAi), the technology of activating the RNAi pathway and the application of this technology to enhance livestock production through increased production effi ciency and prevention of disease. An increased demand for sustainable food production is at the forefront of scientifi c challenges and RNAi technology will undoubtedly play a key role.


Session IV: Production of PregnanciesThe Impact of Gamete Source and Culture Conditions on the Competence of the In Vitro Produced Embryo for Post-Transfer Survival in CattlePeter Hansen, University of Florida, Gainesville, FL, USA

One limitation to the use of in vitro produced embryos in cattle production systems is the fact that pregnancy rates after transfer to recipients are typically lower than when embryos produced in vivo are transferred. Conceptually, the oocyte and sperm from which the embryo is derived could affect competence for post-transfer survival. There are sire differences in embryonic survival after transfer but there is little evidence that an embryo’s ability to establish pregnancy is determined by sex-sorting of spermatozoa by fl ow cytometry. The role of the source of oocyte as a determinant of embryonic survival after transfer has not been examined carefully. Conditions for culture of the embryo after fertilization can have an impact on the ability of the embryo to establish pregnancy after transfer. Among the specifi c molecules produced in the reproductive tract of the cow that have been shown to improve competence of in vitro produced embryos for post-transfer survival are colony-stimulating factor 2, insulin-like growth factor-1 (for recipients exposed to heat stress) and hyaluronan (for less advanced embryos). There is also a report that embryo competence for post-transfer survival can be improved by inclusion of a carbon-activated air fi ltration system in the incubator used to culture embryos. Progress in developing culture systems to improve embryonic competence for survival after transfer would be hastened by development of in vitro assays that accurately predict potential of an embryo to establish pregnancy after transfer. A group of 52 genes has been identifi ed that are differentially expressed in embryos that developed to term vs embryos that did not establish pregnancy. Perhaps, a gene microarray consisting of these genes, alone or in combination with other genes, could be used to screen embryos for competence to establish pregnancy.

Bovine Embryo Transfer Recipient Synchronization and Management in Tropical Environments Pietro Baruselli, University of Sao Paulo, Brazil

Numerous studies have shown that it is possible to manipulate follicular and luteal dynamics, thereby eliminating the need for estrus detection in embryo transfer programs (ET). Fixed-time embryo transfer (FTET) protocols are based on the use of GnRH and PGF or progesterone/progestogen (P4) releasing devices and estradiol. FTET protocols increase the proportions of recipients transferred and pregnancy rates compared with those using PGF followed by ET 7 days after estrus. Furthermore, the addition of eCG to the P4 and estradiol-based FTET protocols resulted in a greater proportion of recipients transferred and higher pregnancy rates. The benefi cial effect of eCG treatment may be related to an increased growth of the dominant follicle and increased plasma P4 concentrations during the subsequent luteal phase. In Bos taurus x Bos indicus recipients pregnancy rates were positively correlated with the diameter of the CL and the number of CL at ET. When repeat breeder Holstein cows were used as recipients, FTET protocols increased number of recipients transferred and pregnancy rates compared to the traditional PGF-based synchronization protocols. In conclusion, the use of FTET protocols eliminates the necessity of estrus detection and results in a greater proportion of recipients transferred and satisfactory pregnancy rates. Thus, it optimizes the use of recipients, reducing labor and animal handling and facilitating the utilization of ET.

Session V: Production of Live OffspringPregnancy Recognition and Abnormal Offspring Syndrome in CattleCharlotte Farin, North Carolina State University, Raleigh, NC, USA

Development of the post-hatching conceptus in ruminants involves a period of morphological expansion driven by complex interactions between the conceptus and its intrauterine environment. As a result of these interactions endometrial physiology is altered, leading to establishment of the pregnancy and continued development of the placenta. Disruption of normal conceptus, fetal and placental development can occur when embryos are exposed to manipulations in vitro or when inappropriate endocrine sequencing occurs in vivo during the pre- and peri-implantation period. This presentation will address the development of the post-hatching bovine conceptus, its interactions with the maternal system and alterations in development which can occur as a result of in vivo and in vitro manipulations of the bovine embryo.

Program


ProgramDelivery of Cloned Offspring: A Zebu Cattle (Bos indicus) ExperienceFlavio Meirelles, University of Sao Paulo, Pirassununga, Brazil

The production of a healthy cloned calf is dependent on a multitude of successful steps, including the reprogram-ming mediated by the oocyte, the development of a functional placenta, the adequate materno-fetal interaction, the establishment of a physiological metabolic setting, and the formation of a complete set of well differentiated cells that will eventually result in well characterized and fully competent tissues and organs. Although nuclear transfer effi ciency improved signifi cantly since the fi rst SCNT derived animal report, there are many descriptions of anomalies concerning cloned calf leading to a high perinatal morbidity and death rate. This article aims to discuss some of our acquired clinical and management experience in perinatal and neonatal procedures for cloned Zebu cattle (B. indicus) that has been successful in improving survival rates in Nellore cloned calves following the application of such ?labor-intensive technology?

Practitioners’ Forum Cattle IVF in South America: A short story of great results…Brazilian Embryo Technology Society (Sociedade Brasileira de Tecnologia de Embriões, SBTE) This two-hour session will highlight the history of massive dissemination of in vitro embryo production in

South America in the last 10 years, which has made the region responsible for half of the cattle embryos produced in the world. The session will begin with an introductory lecture about the history of IVF use in the region, followed by short (15-minute) reports from private and research laboratories, as well as fi eld practitioners, showing their results and current opportunities in this sector. The objective of this forum is to promote discussion among researchers, laboratory staff and fi eld practitioners not only from South America but from other regions of the world.

Session VI: Keynote AddressSystems Biology of Embryogenesis Nathan Price, University of Illinois, Urbana, IL, USA

The development of a complete organism from a single cell involves extraordinarily complex orchestration of biological processes that vary intricately across space and time. Systems biology seeks to describe how all elements of a biological system interact in order to understand, model, and ultimately predict aspects of emergent biological processes. Embryogenesis represents an extraordinary opportunity - and challenge - for the application of systems biology. Systems approaches have already been used successfully to study various aspects of development, from complex intracellular networks to 4D models of organogenesis. Going forward, great advancements and discoveries can be expected from systems approaches applied to embryogenesis and developmental biology.


New Developments in The Practice Of Embryo TransferIETS 2010 Symposium

Saturday, January 9, 2010 Cordoba, Argentina

Bioniche Animal Health is again generously sponsoring a symposium on new developments in the practice of embryo transfer for practitioners and clinical scientists, immediately before the Annual Meeting of the IETS in Cordoba, Argentina. Eight leading researchers and practitioners from Argentina, Uruguay, Brazil, New Zealand and the USA will be presenting new and previously unpublished information. Be sure to get registered early to ensure your attendance.

Scientifi c Program

08:00 Opening comments.

08:30 New approaches to superovulation in the cow.Dr. Gabriel Bo, Argentina

09:15 The use of knowledge on LH receptors to improve superstimulatory treatments in cattle. Dr. Ciro Barros, Brazil

10:00 Coffee break

10:30 New approaches to superovulation and embryo transfer in small ruminants.Dr. Alejo Menchaca, Uruguay

11:15 Synthetic media for culture, freezing and vitrifi cation of bovine embryos.Dr. John Hasler, USA

12:00 Lunch

13:30 Factors affecting fertilization and early embryo quality in single- and superovulated dairy cattle. Dr. Roberto Sartori, Brazil

14:15 The use of equine chorionic gonadotrophin in synchronized AI of seasonal breeding, pasture-based, anestrous dairy cattle. Dr. Mark Bryan, New Zealand

15:00 Coffee Break

15:30 Practical applications of new research information in the practice of bovine embryo transfer. Dr. Charles Looney, USA

16:15 Brief introduction to whole genome selection in cattle using SNPs. Dr. George Seidel, USA

17:00 General discussion. Moderator, Reuben Mapletoft, Canada

18:00 Close

Pre-Conference Satellite Symposium


Pre-Conference Satellite Workshop

“Current Progress in Domestic Animals Embryonic Stem Cell Research”Organized by The Domestic Animal Biomedical Embryology Committee

Saturday, January 9, 2010 Sheraton Cordoba Hotel

14.00 - 14.15 Welcome and IntroductionFulvio Gandolfi

14.15 - 14.45 The role of epigenetic modifi ers for the establishment of mammalian pluripotent cell linesPaul J. Verma

14.45 - 15.00 Sheep embryonic germ cellsSergio Ledda

15.00 - 15.30 Mankind’s best friend: Canine embryonic stem cellsMarlon Schneider

15.30 - 15.45 Derivation of cat embryonic stem-like cells from in vitro-produced blastocystsMartha Gomez

15.45 - 16.15 Coffee break

16.15 - 16.30 Equine embryos and embryonic stem cells: Defi ning true markers of pluripotencyDamien Paris

16.30 - 17.00 Goat embryonic stem cellsEsmail Behboodi

17.00 - 17.15 Pig embryonic stem cellsTiziana Brevini

17.15 - 17.45 Development of putative induced pluripotent stem cells in swineJorge A. Piedrahita

17.45 - 18.00 Conclusions


Post-Conference Workshop for AuthorsWorkshop for Authors

Publishing Scientifi c Papers in EnglishJanuary 13-14, 2010

John P. Kastelic, DVM, PhD, Co-Editor-in-Chief, Theriogenology

Many manuscripts are delayed or rejected due to poor experimental design, analysis and presentation of data, and writing. This workshop covers how to plan and conduct research, analyze and present data, write a paper, and interact with editors and reviewers. In addition to presentations of principles and common errors, there will be exercises and interactive discussions. Course notes and certifi cates of attendance will issued.

This workshop is primarily designed for those for whom English is a second language. Therefore, English syntax, grammar and punctuation will be reviewed. However, this workshop will also be valuable for those for whom English is their native language, especially students and young scientists.

The workshop will be held from 08:30 to 17:00 on Wednesday and Thursday, January 13 and 14, at the Universidad Católica de Córdoba, Obispo Trejo 323, Cordoba. This university is downtown and is walking distance (approximately 12 blocks) from the main conference hotel for the 2010 IETS meeting.

Class size is limited, so please register early to ensure your place. Registration fees are payable to IETS via the registration form (www.iets.org/2010). The reduced early registration fee (US$150 for IETS members listed in current membership directory; US$200 for nonmembers) must be received before 11 November 2009. The On-Site registration fee is US$200 for IETS members and US$250 for non-members (if space is available). Student registration (undergraduate or graduate students and post-doctoral fellows) is US$125, pre-paid or US$175 at the door. The registration fee does not include meals.

This workshop will be conducted by Dr. Kastelic. He has more than 20 years of experience conducting research and publishing papers, and is author or co-author of >100 peer-reviewed articles and >200 abstracts, proceedings and other articles. In addition, he has considerable experience as a reviewer and editor, including serving as Co-Editor-in-Chief of Theriogenology since 2003. He has done presentations and workshops on science and science writing in nine countries on four continents. It has been his privilege to visit South America on several occasions, primarily to conduct collaborative research and to deliver lectures and workshops on science and science writing.

For information about the workshop, contact Dr. Kastelic: [email protected] 403-317-2236 (voice) 403-382-3156 (fax)

Registration and fees: www.iets.org/2010 [email protected] (voice)


Special Events

Open Meeting of the Health And Safety Advisory CommitteeSunday, January 10, 201018:00 - 19:00Lugones

Welcome ReceptionSunday, January 10, 201020:00Sheraton Cordoba Hotel - Lugones Room and Lugones FoyerThe Welcome Reception will take place on January 10, 2010, at 20:00. Wines Cocktails and Hors d’oeuvres will be served at the Lugones room and among the exhibits in the Foyer.

Open Meeting of the Domestic Animal Biomedical Embryology CommitteeMonday, January 11, 201018:00 - 19:00Suquia

Open Meeting of the Companion Animals, Non-Domestic & Endangered Species CommitteeMonday, January 11, 201018:00 - 19:00Lugones

Practitioner’s Forum - Commercial IVPTuesday, January 12, 201010:30 - 12:30Lugones

8th IETS Annual Running CompetitionTuesday, January 12, 201016:30 - 17:30Meet near the registration desk. The course will take participants around beautiful Cordoba. We look forward to seeing you participate.

Closing PartyTuesday, January 12, 201019:00 - until the last bus go homeEstancia La Angelina (30 minutes from Downtown Córdoba)http://www.laangelina.netAttendees will be transported by bus from the main conference hotels (Sheraton Córdoba Hotel, Amerian Córdoba Park, Holiday Inn, Orfeo Suites, King David Flat Hotel, NH Panorama and Urbano) to the party.The service will consist of different Argentine dishes and beverages, including beef asado and red wine. We will have music all night for all those wishing to stay late. Buses will begin to return to the hotels at 23.00. The last bus will depart before day break.


REMINDEREarly registration deadline is November 11, 2009

Córdoba Information and AttractionsThe province of Córdoba is located in the centre of the continental territory of the Argentina Republic. Córdoba is bounded by the provinces of Santiago del Estero and Catamarca to the north, La Rioja and San Luis to the west, La Pampa and Buenos Aires to the south, and Santa Fe to the east. The province of Córdoba has become a favored tourist destination, thanks to its beautiful landscapes, majestic mountains ranges and mildness of climate. The Province of Cordoba offers everything for the organization of events, conferences, exhibitions and fairs. Its favorable climate throughout the year and infrastructure is ready to ensure visitors the highest quality attention. Its shopping centers and gastronomic services, its intense cultural activity and nightlife of theaters, casinos, discos and nightclubs. Cordoba offers the best options for combining work and pleasure; a few minutes away from downtown you can take refuge in a preserved landscape, fi nd monuments with exciting stories and practice the most diverse activities along with lakes and mountains.

The city of CórdobaFounded on July 6, 1573, by Jerónimo Luis de Cabrera, on the banks of Río Suquía (Suquía River), Córdoba is a must for tourists. Located in the center of Argentina between 360 and 480 meters above sea level, Córdoba has more than 1,300,000 inhabitants and a dynamic economy which makes it the second most important city in Argentina. Because of its strategic location, it constitutes a great national and international communication hub. Another distinctive feature is its numerous universities, both public and private and great university population. The National University of Córdoba was founded in 1613.Those who come to Córdoba will be able to walk around its historical downtown - the fi rst pedestrian area built in the country, and discover the treasures of its colonial architecture surrounded by outstanding beauty.Córdoba offers a vast network of services, accommodation facilities (both 4 and 5 star hotels), a downtown commercial area, three large shopping centers, various supermarkets and modern movie theaters. Also, Córdoba is the venue of numerous conferences, cultural and sport activities all year round. There are wonderful restaurants offering typical dishes as well as international menus. Night life has a special fl avor: several bars, pubs, “peñas” (local folk music gatherings) and dance clubs. Tourists will feel safe and comfortable in this city. For those who would rather combine sport with the peace of the hills, there are places that offer personalized service, typical food, excellent indoor space and a wide range of activities: horseback riding, trekking, 4x4 tours, etc. Ideal for families and companions.

More information about Córdoba available at: www.iets.org/2010

Companion's TourThere are several tours organized during the Annual Conference. For more details please contact:Site Argentina DMC - Travel AgencyPhone: +54 351 5891584Cel phone: + 54 351 (9) 3256371 + 54 351 (9) [email protected]


Job Resource Center

Looking for that perfect job?

Looking for the perfect employee?

Let IETS end your search at the Job Resource Center at the 2010 Annual Conference in Cordoba

If you are looking for the perfect job, please bring 10 copies of your resume to the IETS Annual Conference in Cordoba. Graduates, undergraduates, and professionals looking for a job are encouraged to participate. If you are unable to attend the confer-ence, but would like to participate in the Job Resource Center, please send your 10 resumes by December 1, 2009 to: IETS Job Resource Center, 2441 Village Green Place, Champaign, IL 61822, USA.

If your company is looking for the perfect employee, please bring 10 copies of your position announcement which includes details such as qualifi cations and dead-lines to the IETS Annual Conference in Cordoba. Companies, universities, and gov-ernmental agencies are encouraged to participate. If you are unable to attend the conference, but would like to participate in the Job Resource Center, please send your 10 position announcements by December 1, 2009 to: IETS Job Resource Center, 2441 Village Green Place, Champaign, IL 61822, USA.


IETS Membership Application

Join the IETS now and save

on registration fees for the

2010 Annual Conference in

Cordoba, Argentina

By becoming a member of the IETS now, you will enjoy a reduced rate when you register for the

2010 Annual Conference.

As a member, you will receive the on-line quarterly Embryo Transfer Newsletter of the IETS, on-line subscription to Reproduction, Fertility and Development, an on-line searchable membership directory, discounted rates for all sales items (books, videos & slides) offered by IETS and discounts and advance information on future

conferences and events.

You will also be entitled to reduced subscription rates to Theriogenology and Animal Reproduction Science.

Membership ApplicationInternational Embryo Transfer Society

2010 Annual Conference Special

___ Yes! I'd like to become a new member of the IETS now for 2010 and save when I register for the conference. Name Last First M.I.

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City State/Province Zip/Postal Code CountryPhone FAX E-mail Membership Type___ Full Member US $160*___ Associate Member US $160*___ Student Member US $80* *Includes a $10 administrative fee for joining with the Annual Conference.Payment___Check #______________MasterCard ___Visa ___AMEX ___Discover Card #

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Please mail with registration and remittance to:IETS

2441 Village Green PlaceChampaign, IL 61822 USA

(217) 398-4697, FAX: (217) 398-4119E-mail: [email protected]

Web Site: http://www.iets.org


2010 MEETING SPONSORSas of September 21, 2009

GOLD ($8,000+)

SILVER ($5000-$7000)

BRONZE ($2000-$4999)

FRIEND (up to $1999)

Documents

IETS - Registration Book