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Immunology and Inflammation

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Genetically engineered and

immune system engrafted

models offer a unique

opportunity for advancing

immunology and inflammation-

related research.

The immune system plays a pivotal role in the pathogenesis of many diseases, including autoimmune, allergic and infectious diseases, cancer, and atherosclerosis. Taconic Biosciences provides models and services to help explore those connections and model human disease.

Immunology and InflammationSolutions

Taconic Biosciences provides easy access to our exclusive portfolio of immunology and inflammation models designed to help grow and accelerate our clients’ drug discovery pipeline.

In addition to a broad spectrum of mechanistic and disease models in immunology and inflammation, Taconic offers a range of humanized models, including models with human gene replacement or mutations, and cell and tissue engraftment. The use of human immune system engrafted mice has proven to be advantageous in a wide variety of applications including immunomodulatory therapy, and autoimmune and infectious disease studies. Taconic also leads the industry in microbiome research—providing the highest number of health standards, several germ-free models, and related microbiome services including fecal microbiota transplantation and germ-free derivations. This platform allows researchers the ability to study the impact of the microbiome on immunology and inflammation targets and therapeutics.




There is growing evidence that segmented filamentous bacteria (SFB) can alter the immune system. Knowing whether SFB is present or absent in a model allows the investigator to control for this variable, making results more reproducible. While SFB is only one component of the gut microbiome, for many models the impact could be significant. Taconic includes SFB on all health reports. The Taconic Germ Free, Defined Flora, and Excluded Flora health designations exclude SFB.

The microbiome has become a major focus of many studies in the realm of immunology, oncology and inflammation. Taconic offers three key models, Black 6, BALB/c, and Swiss Webster, as off-the-shelf germ-free mice for immediate delivery in typical study sizes. Germ-free mice are devoid of all microorganisms (as determined within the limitations of the detection methods available), including bacteria, fungi and viruses; they have no microbiome. Strict Taconic husbandry and testing programs ensure these mice remain germ-free. Germ-free mice may be maintained as germ-free long-term or they may be associated with a custom microbiota. Taconic provides custom association services using customer-provided materials as well as custom germ-free derivation services and husbandry.

GERM FREE MODELS

SEGMENTED FILAMENTOUS BACTERIA (SFB)




TRADITIONAL MODELS

BLACK 6 INBRED MOUSE C57BL/6NTac

• The most widely used inbred as the

genetic background for transgenic

and mutant mice.

• Popular in research applications of

oncology, immunology and toxicology.

• Now available at the Germ Free health

standard in addition to Excluded Flora,

and Murine Pathogen Free™ health

profiles. The wide variety of health

designations available makes the B6

model ideal for microbiome studies.

• B6NTac is an excellent choice for the

chronic experimental autoimmune

encephalomyelitis model of

multiple sclerosis.

• May also be used for DSS-induced

colitis, induced model of systemic

lupus erythematosus (SLE) and

CIA-induced arthritis.

• Th2-biased.MODEL NUMBER B6

NOMENCLATURE

BALB/cINBRED MOUSE

• Often used for the production of

monoclonal antibodies using hybridomas

of BALB/c spleen cell origin.

• Exhibits good breeding performance and

long reproductive life span.

• Used in ovalbumin induced

hypersensitivity.

• Used in studies of innate and adaptive

immunity.

• Applications in colitis, allergy and asthma

and infectious disease studies.

• Th2-biased.

MODEL NUMBER BALB

BALB/cAnNTac

BALB/c

NOMENCLATURE

MODEL NUMBER BALJBO

BALB/cJBomTac

BALB/c Bom

NOMENCLATURE



DBA/1 INBRED MOUSE DBA/1JBomTac

• Suitable general-purpose research

model for immunology and

inflammation research.

• Widely used as a model of

autoimmunity, especially for collagen-

induced studies of rheumatoid arthritis.

• High incidence of mammary tumors.

MODEL NUMBER DBA1BO

NOMENCLATURE

B6.SJLCONGENIC MOUSE B6.SJL-Ptprca/BoyAiTac

• Genetically similar to the C57BL/6Boy

strain except that it carries the Ptprca

allele (protein-tyrosine phosphatase,

receptor type c locus previously known

as CD45.1, Ly5.1) and the Pep3b allele

from the SJL/J strain.

• The unique lymphocyte cell surface

antigen produced by Ptprca makes

this strain useful for immunological

adoptive transfer experiments and

useful as a background for GEMs

used in adoptive transfer studies.

MODEL NUMBER 4007

NOMENCLATURE




SPONTANEOUS MUTANT MODELS

NIH nudeSPONTANEOUS MUTANT RAT

T CELL DEFICIENT NTac:NIH-Foxn1rnu

• Exhibits deficient T cell activity with

lymph nodes and Peyers patches

showing lymphocyte depletion in

T-lymphocyte dependent regions.

• In this outbred immunodeficient

model, the whiskers are present in the

homozygous nude rat, but present

as bent with some short hairs on the

head and occasionally on the rest of

the body (with age some hair growth

occurs, but rats become hairless again

within a few weeks).

• Skin of homozygous rat is generally

pigmented, but occasionally an

albino rat is seen.

• Good xenograft host.

• Used in infectious disease studies and

as a model of IBS.

MODEL NUMBER NIHRNU

NOMENCLATURE

B6 nudeSPONTANEOUS MUTANT MOUSE

T CELL DEFICIENT B6.Cg/NTac-Foxn1nu NE10

• The autosomal recessive nude gene in

homozygous (nu/nu) mice causes the

lack of fur and an abnormal thymus.

Deficiency in T cell function allows

athymic mice to accept and grow

xenografts as well as allografts of

normal and malignant tissues.

• Foxn1nu mutation backcrossed to

the C57BL/6NTac inbred strain for

ten generations.

• An appropriate model for adoptive

transfer experiments among other

strains on the B6 background.

• Used to study infectious diseases

including HIV and other viral infections.

MODEL NUMBER B6NU

NOMENCLATURE

BALB/c nudeSPONTANEOUS MUTANT MOUSE

T CELL DEFICIENTC.Cg/AnNTac-Foxn1nu NE9

• Foxn1nu mutation backcrossed to the

BALB/cAnN inbred strain for nine

generations.

• Available in two health designations:

Defined Flora from gnotobiotic

isolators and Restricted Flora™ from

Isolated Barrier Units™.

• Appropriate model for adoptive

transfer experiments among other

strains on the BALB/c background.

MODEL NUMBER BALBNU

NOMENCLATURE



NCr nudeSPONTANEOUS MUTANT MOUSE

T CELL DEFICIENT CrTac:NCr-Foxn1nu

• Outbred background originated

from an accidental cross between

the BALB/c inbred nude and NIH(S)

outbred nude mice.

• The standard athymic model for

National Cancer Institute (NCI)

studies as well as many

pharmaceutical and institutional

oncology screening programs.

• Used in autoimmune disease studies

and transplantation.

MODEL NUMBER NCRNU

NOMENCLATURE

NMRI nudeSPONTANEOUS MUTANT MOUSE

T CELL DEFICIENTBomTac:NMRI-Foxn1nu

• Foxn1nu mutation backcrossed to the

NMRI outbred stock.

• A good general purpose outbred nude. • Used in autoimmune disease studies

and transplantation.

MODEL NUMBER NMRINU

NOMENCLATURE

C.B-17 scidSPONTANEOUS MUTANT MOUSE

T & B CELL DEFICIENTC.B-Igh-1b/IcrTac-Prkdcscid

• Mice homozygous for the Prkdcscid

mutation lack both T and B cells due

to a defect in V(D)J recombination.

Therefore, they easily accept foreign

tissue transplants, including human

tumors, making them effective models

for testing new cancer treatments and

as hosts for human immune system

tissues (i.e. SCID-hu).

• This is the original congenic

background strain on which Dr. Mel

Bosma discovered the spontaneous

scid mutation.

• Available at two health designations:

Defined Flora from gnotobiotic

isolators and Restricted Flora™ from

Isolated Barrier Units™.

• Used in studies of adaptive immunity

and infectious disease.

MODEL NUMBER CB17SC

NOMENCLATURE



NOD scidSPONTANEOUS MUTANT MOUSE

T&B CELL DEFICIENT AND REDUCED NK FUNCTIONNOD/MrkBomTac-Prkdcscid

• The scid mutation has been transferred

onto a diabetes-susceptible Non-Obese

Diabetic (NOD) background, making it

a great model for metabolic disorder

research. Note: The NOD scid does not

develop diabetes.

• The multiple immunity defects unique

to this model provide an excellent

system for reconstitution with human

hematopoietic cells, making it an

exceptional model for HIV-1 research

and gene therapy studies.

• Useful model for cancer research into

increased tumor incidence, particularly

lymphomas and thymic tumors.

• Short life span of 8-9 months due to

lethal thymic lymphomas.

• Reduced NK cell activity due to

NOD background.

MODEL NUMBER NODSC

NOMENCLATURE

Scid-beigeSPONTANEOUS MUTANT MOUSE

T&B CELL DEFICIENT AND REDUCED NK CELLSC.B-Igh-1b/GbmsTac-Prkdcscid-Lystbg N7

• The mutations were backcrossed

seven generations to the congenic

C.B-17 background.

• This double mutant model carries the

scid mutation which causes a lack of

both T and B lymphocytes due to a

defect in V(D)J recombination.

• Model also carries the beige mutation

which results in cytotoxic T cell

and macrophage defects as well

as selective impairment of NK cell

functions. As a result, scid-beige mice

are an improved model for some types

of xenotransplants.

• The scid-beige strain has a lower

incidence of serum Ig relative to the

C.B-17 scid strain.

MODEL NUMBER CBSCBG

NOMENCLATURE

ICR scidSPONTANEOUS MUTANT MOUSE

T & B CELL DEFICIENTIcrTac:ICR-Prkdcscid

• Equivalent to the C.B-17 scid in severity

of immunodeficiency, this outbred

background exhibits a significantly

reduced incidence of spontaneous Ig

production (leakiness).

• Gains weight faster than the C.B-17

scid which makes the ICR scid better

suited for ascites production from

heterohybridomas and as a host for

tumor lines.

• Mice homozygous for the Prkdcscid

mutation lack both T and B cells due

to a defect in V(D)J recombination.

Therefore, they easily accept foreign

tissue transplants, including human

tumors, making them effective models

for testing new cancer treatments.

SCID mice are also useful for examining

the relationship between immunity

and disease.

MODEL NUMBER ICRSC

NOMENCLATURE



IMMUNOLOGY RANGE COMPARISON TABLE

Listed below is information specific to Taconic’s Murine Pathogen Free™ (MPF™), Restricted Flora™ (RF™), Excluded Flora (EF),

and Defined Flora (DF) immunodeficient rodents. Taconic currently produces immunodeficient models (both spontaneous and

genetically modified mutations) on different background strains (and coat colors) and of differing health/microbiological profiles.

To inquire about availability of your model of interest at particular health designations, visit taconic.com/health-standards

MODEL NUMBER MODEL NAME COAT COLOR CELL DEFICIENCIES OTHER IMMUNODEFICIENCIES

BALBNU BALB/c nude mouse

B6NU B6 nude mouse

NCRNU NCr nude mouse

NMRINU NMRI nude mouse

NIHRNU NIH nude rat

1147 Jh mouse

CB17SC C.B.17 scid mouse

ICRSC ICR scid mouse

NODSC NOD scid mouse

Shows reduced NK function

Defective dendritic cells & macrophages and reduced complement activity.

601 Rag2 (BALB/c) mouse

RAGN12 Rag2 (C57BL/6) mouse

CBSCBG Scid-beige mouse

4111Rag2/Il2rg Double Knockout Mouse Dysfunctional dendritic cells

11503 CIEA BRG mouse Dysfunctional dendritic cells

NOG CIEA NOG mouse® Reduced complement activity, dysfunctional macrophages and dendritic cells, deficiencies in immune signaling, including cytokine production.The most immune deficient mouse available.

B2MN12 ß2m (C57BL/6) mouse Depletion of CD8+ T cells

ABBN12 Abb (C57BL/6) mouse Depletion of CD8+ T cells

11490 Rag2/OT-II mouse Expresses altered T cell population

2334 Rag2/OT-I mouseExpresses altered T cell population

White-bellied agouti

COAT COLOR

Black and White Nude Albino Nude

Black Nude

KEY:

Black

Albino

CELL DEFICIENCIES

T Cell Deficient

B Cell Deficient

NK Cell Deficient

MHC Deficient




GENETICALLY ENGINEERED MODELS

To keep up with today’s fast-paced research, Taconic recognizes our customers’ need for speed and accessibility when it comes to genetically engineered models (GEMs) for immunology research. This portfolio provides quick access to advanced research models that are fully licensed and available in typical study quantities. Each GEM carries a license granting our customers the intellectual property rights to use the GEM in their research. Take advantage of Taconic’s GEM portfolio to avoid the challenges and cost of locating, licensing, and breeding the models you need.

GEMs

Abb (H2-Ab1)CONSTITUTIVE KNOCKOUT

C57BL/6 BACKGROUNDB6.129-H2-Ab1tm1Gru N12

• Contains a disruption of the

H2-Ab1 gene.

• Expresses no A or E MHC class II

molecules and therefore lacks

most CD4+ T cells.

• Useful in transplantation, gene therapy

and immunological disease research.

MODEL NUMBER ABBN12

NOMENCLATURE

B2m (β2m)CONSTITUTIVE KNOCKOUT

C57BL/6 BACKGROUND

• Contains a disruption of the

beta-2-microglobulin gene.

• These MHC class I deficient mice are

depleted of CD8+ T cells (lack the

NK1.1+ CD4+ T cell population as well).

• Useful in transplantation, gene therapy,


MODEL NUMBER B2MN12

NOMENCLATURE

B6.129-B2mtm1Jae N12

CRYOPRESERVED



Abb–B2mCONSTITUTIVE KNOCKOUT

C57BL/6 BACKGROUNDB6.129-H2-Ab1tm1Gru B2mtm1Jae N17

• This double targeted mutation contains

disruption of both H2-Ab1 and B2m

genes, combining the characteristics

of the two individual targeted

mutation models.

• Useful in transplantation, gene therapy,


MODEL NUMBER 4080

NOMENCLATURE

Ccr2CONSTITUTIVE KNOCKOUT

C57BL/6 BACKGROUNDB6.129P2-Ccr2tm1Mae N10

• Disruption of the Ccr2 gene results in

defects in macrophage trafficking.

• Useful for elucidating the function of

cells expressing Ccr2 during immune

and inflammatory processes.

• Useful for the study of asthma, arthritis,

and colitis.

MODEL NUMBER 3736

NOMENCLATURE

COX-1CONSTITUTIVE KNOCKOUT

MIXED C57BL/6 AND 129P2 BACKGROUNDB6;129P2-Ptgs1tm1Unc

• Contains a disruption of the Ptgs1 gene,

one of the prostaglandin endoperoxide

synthase/cyclooxygenase genes,

preventing synthesis of the

cyclooxygenase-1 (COX-1) protein.

• Homozygous mice have a significantly

reduced inflammatory response to

arachidonic acid, but not to other

inflammatory agents.

• Mice develop normally, appear healthy,

and do not exhibit gross or microscopic

gastric lesions.

• Useful to study cyclooxygenase

inhibitors and NSAIDs and to examine

the role of cyclooxygenae in

inflammatory disease.

MODEL NUMBER 2180

NOMENCLATURE

CRYOPRESERVED

CRYOPRESERVED

CRYOPRESERVED



COX-2CONSTITUTIVE KNOCKOUT

MIXED C57BL/6 AND 129P2 BACKGROUNDB6;129P2-Ptgs2tm1Unc

• Contains a disruption of the Ptgs2

gene, one of the prostaglandin

endoperoxide synthase/

cyclooxygenase genes, preventing

synthesis of the cyclooxygenase-2

(COX-2) protein.

• Homozygotes develop peritoneal

lesions and exhibit severe, progressive

renal pathology and lesions of varying

severity that cause a progressive

deterioration with aging.

• Homozygotes exhibit normal

inflammatory response to bacterial

assault and to arachidonic acid and

other inflammatory agents.

• Useful to examine the redundant

and compensatory responses of

the cyclooxygenase gene, to study

cyclooxygenase inhibitors and NSAIDs

and to examine the role of COX

enzymes in inflammatory disease.

MODEL NUMBER 2181

NOMENCLATURE

Fcer1g (FcRγ)CONSTITUTIVE KNOCKOUT

• Deficient in the γ chain

subunit of the FcgRI, FcgRIII

and FceRI receptors.

• The deleted gamma chain

subunit is essential for cell

surface expression of

Fc receptors.

• Macrophages, neutrophils,

mast cells, basophils, and

NK cells are functionally

impaired, due to the lack of

the Fc receptors.

• The mice have normal T cell

thymic development and

peripheral T cell function.

• Useful in determining the

role of structurally similar

Fc receptors in mediating

effector immune responses

and studying the pleiotropic

role of the γ chain subunit.

• Mice exhibit several different

types of immunodeficiency

making them useful for

studies to distinguish the

role of the Fc receptors in

antibody-mediated effector

responses and to evaluate

the contribution of IgG

and IgE triggered effector

pathways.

MODEL NUMBER 583

B6.129P2-Fcer1gtm1Rav N12

C57BL/6 BACKGROUND

THESE MICE CARRY THE H2b HAPLOTYPE

NOMENCLATURE

MODEL NUMBER 584

C.129P2(B6)-Fcer1gtm1Rav N12

BALB/c BACKGROUND

THESE MICE CARRY THE H2d HAPLOTYPE

NOMENCLATURE

Fcgr2b (FcγRII)CONSTITUTIVE KNOCKOUT

• Deficient in FcgRIIβ protein,

which is a low affinity

immunoglobulin G receptor.

• The FcgRIIβ protein inhibits

the activation of disparate

effector functions such as

phagocytosis, antibody

dependent cytotoxicity, and

release of inflammatory

mediators. It is also known

to function as an inhibitory

receptor on B cells and

mast cells.

• This inhibitory pathway

for activation of several

immune effector responses

is dysfunctional, resulting

in the inability to regulate

antibody levels in response

to antigenic stimuli

dependent on IgG

immune complexes.

• Useful for studying the

feedback inhibition

pathways that regulate

antibody production and

in studies of allergic and

autoimmune disorders.

MODEL NUMBER 580

B6.129S4-Fcgr2btm1TtK N12

C57BL/6 BACKGROUND

THESE MICE CARRY THE H2b HAPLOTYPE

NOMENCLATURE

MODEL NUMBER 579

C.129S4(B6)-Fcgr2btm1TtK/cAnNTac N12

BALB/c BACKGROUND

THESE MICE CARRY THE H2d HAPLOTYPE

NOMENCLATURE

CRYOPRESERVED



HLA-A1RANDOM TRANSGENIC

CB6F1 BACKGROUNDCB6F1-Tg(HLA-A*0101/H2-Kb)A1.01

• Carries a transgene consisting of

fragments of the human HLA-A*0101

gene and mouse H2-Kb gene which

encodes a chimeric class I molecule

consisting of the human HLA-A1

leader, α1 and α2 domains ligated to

the murine α3, transmembrane and

cytoplasmic H2-Kb domains.

• Expresses the chimeric HLA-A1

class I molecule on the surface of

T and B cells.

• Represents the human HLA-A1

supertype.

• Useful for infectious disease research,

vaccine development and testing,

safety and immunogenicity testing,

as well as research directed towards

oncology and autoimmune disorders.

• Permits identification of epitopes

restricted to the HLA-A1 supertype.

MODEL NUMBER 9662

NOMENCLATURE

HLA-A2.1RANDOM TRANSGENIC

CB6F1 BACKGROUNDCB6F1-Tg(HLA-A*0201/H2-Kb)A*0201





consisting of the human HLA-A2.1




• Expresses the chimeric HLA-A2.1

class I molecule on the surface of T

and B cells.

• Represents the human HLA-A2

supertype.



safety and immunogenicity testing





MODEL NUMBER 9659

NOMENCLATURE













T and B cells.

• Represents the human HLA-A3 supertype.








• This model is produced upon order and

will not be available for delivery at least

6 weeks from order placement.

MODEL NUMBER 9660

NOMENCLATURE

CRYOPRESERVED















T and B cells.

• Represents the human HLA-A24 supertype.








• This model is produced upon order and

will not be available for delivery at least

6 weeks from order placement.

MODEL NUMBER 9663

NOMENCLATURE

HLA-B7

HLA-B44

CONSTITUTIVE KNOCKOUT / RANDOM TRANSGENIC

CB6F1 BACKGROUND

RANDOM TRANSGENIC

CB6F1 BACKGROUND

CB6F1-B2mtm1Unc Tg(B2M)55Hpl

CB6F1-Tg(HLA-B*4002/H2-Kb)B44.01


fragments of the human HLA-B*0702



consisting of the human HLA-B7




• Expresses the chimeric HLA-B7 class I

molecule on the surface of T and

B cells.

• Represents the human HLA-B7

supertype.







restricted to the HLA-B7 supertype.


fragments of the human HLA-B*4002



consisting of the human HLA-B44




• Expresses the chimeric HLA-B44


T and B cells.

• Represents the human HLA-B44

supertype.







restricted to the HLA-B44 supertype.

MODEL NUMBER 9661

MODEL NUMBER 9664

NOMENCLATURE

NOMENCLATURE

CRYOPRESERVED

CRYOPRESERVED



Abb Knockout/Transgenic HLA-DR4CONSTITUTIVE KNOCKOUT / RANDOM TRANSGENIC

C57BL/6 BACKGROUND

B6.129S2-H2-Ab1tm1Gru Tg(HLA-DRA/H2-Ea,

• The HLA-DR4 allele is associated with

the development of autoimmune

diseases like rheumatoid arthritis

and multiple sclerosis.

• Expresses a hybrid MHC class II

molecule with the peptide binding

domains of human HLA-DRA and

HLA-DRB*0401 and the membrane

proximal domains of mouse I-E (H2-E).

• Useful for studies of autoimmune

disease and vaccine research.

MODEL NUMBER 4149

NOMENCLATURE

JhCONSTITUTIVE KNOCKOUT

STOCK BACKGROUNDSTOCK Igh-Jtm1Dhu N?+N2

• Carries a deletion of the endogenous

murine J segments of the Ig heavy

chain locus.

• In homozygotes, all four JH gene

segments are absent, resulting in

cells that cannot produce a complete,

recombined version of the variable

region of the heavy chain.

• Have no detectable IgM or IgG in

the sera.

• A low level (about 1% of normal) of

rearrangement of the light chain

kappa gene family is detected in

total bone marrow.

• Cells of the B lineage are drastically

altered both in developmental

progression and in cell quantity.

• Contain no mature (immunoglobulin-

bearing) B lymphocytes in the spleen,

bone marrow, lymph nodes, peripheral

blood, or peritoneum.

• Useful for studying non-B cell activity

in pathogen-induced disease and

mechanisms of antibody gene

assembly and expression.

• T lymphocyte development appears

to proceed normally, based on surface

phenotype and quantity of cells in

the spleen; splenic lymphocytes

are enriched for T cells due to the

B cell deficit.

• Provides a null background useful for

gene replacement experimentation

in normal immune response and

autoimmune diseases.

MODEL NUMBER 1147

NOMENCLATURE

Rag2/II2rg Double KnockoutCONSTITUTIVE KNOCKOUT

MIXED BACKGROUND

B10;B6-Rag2tm1Fwa IIrgtm1Wjl

• Lacks mature T, B, and NK cells.

• Applications include xenografts,

transplantation of allogeneic or

xenogeneic stem cells and research on

the role of NK cells in host resistance to

tumors and infectious agents.

• Not appropriate for studies involving

engraftment of human hematopoietic

stem cells.

• Very useful for transplanting allogeneic

or xenogeneic stem cells which are

often rejected by NK cells.

• Available at the Excluded Flora (EF)

health standard.

MODEL NUMBER 4111

NOMENCLATURE



Rag2/OT-ICONSTITUTIVE KNOCKOUT/

RANDOM TRANSGENICB6.129S6-Rag2tm1Fwa Tg(TcraTcrb)1100Mjb

• Carries a transgene that encodes a

T cell receptor (Vα2-Jα26 and

Vβ5-Dβ2-Jβ.6) specific for a

chicken ovalbumin peptide

fragment (257-264) presented

by the MHC class I molecule H2-Kb.

• Deficient in the recombination

reactivating gene 2 (Rag2) and

therefore does not develop mature

T or B cells expressing endogenous

T cell receptors.

• Virtually all peripheral CD8+ cells

express the transgene.

• Useful as a source of homogeneous

donor CD8+ T cells for in vivo adoptive

transfer studies to investigate T cell

development, activation, memory

and tolerance.

MODEL NUMBER 2334

NOMENCLATURE

MODEL NUMBER 601

C.129S6(B6)-Rag2tm1Fwa N12

BALB/c BACKGROUND

NOMENCLATURE

MODEL NUMBER RAGN12

B6.129S6-Rag2tm1Fwa N12

C57BL/6NTac BACKGROUND

NOMENCLATURE

• Backcrossed twelve generations (N12) to a

BALB/cAnNTac.

• Carries the H2d haplotype.

• Available at Excluded Flora (EF) health standard.

• Backcrossed twelve generations (N12) to a

C57BL/6NTac inbred background.

• Carries the H2b haplotype.

• Available at Murine Pathogen Free™ (MTF™) and

Excluded Flora (EF) health standards.

Rag2CONSTITUTIVE KNOCKOUT

T & B CELL DEFICIENT MODEL

• Contains a disruption of the recombination

activating gene 2 (Rag2).

• Homozygous mice exhibit total inability

to initiate V(D)J rearrangement and fail

to generate mature T or B lymphocytes.

• Useful for vaccine development,

transplantation studies and

hematopoiesis research.

• Rag2 knockouts may be used for

adoptive transfer-induced IBD studies.

MODEL NUMBER RAG2

129S6/SvEvTac-Rag2tm1Fwa

129S6 BACKGROUND

NOMENCLATURE

• The 129S6 strain was the original strain in which the

Rag2 targeted mutation was created.

MODEL NUMBER 461

B6.SJL(129S6)-Ptprca/BoyCrTac-Rag2tm1Fwa N10

B6.SJL-Ptprca BACKGROUND

NOMENCLATURE

• The Ptprca marker on hematopoietic cells of this

congenic strain allows for their identification in

immunological adoptive transfer experiments.

• Similar to C57BL/6 with the H2b haplotype, but

carries Ptprca and Pep3b genes from SJL strain.

CRYOPRESERVED CRYOPRESERVED



Rag2/OT-IICONSTITUTIVE KNOCKOUT/ RANDOM TRANSGENIC

C57BL/6 BACKGROUNDB6.129S6-Rag2tm1Fwa Tg(TcraTcrb)425Cbn

• Carries a transgene that encodes a T

cell receptor (Vα2 and Vβ5) specific for

a chicken ovalbumin peptide fragment

(323-339) presented by the MHC

class II molecule H2-Ab1 (I-Ab).

• Deficient in the recombination

reactivating gene 2 (Rag2) and does

not develop mature T or B cells

expressing endogenous T cell receptors.

• Virtually all peripheral CD4+ cells

express the transgene.

• Useful as a source of homogeneous

donor CD4+ T cells for in vivo adoptive

transfer studies to investigate T cell

development, activation, memory,

and tolerance.

MODEL NUMBER 11490

NOMENCLATURE

TNF-αRANDOM TRANSGENIC

INFLAMMATORY ARTHRITIS MODEL

C57BL/6NTac BACKGROUND

B6.Cg(SJL)-Tg(TNF) N21+?

• Expresses the human tumor necrosis

factor α (TNF-α) transgene, a cytokine

implicated in the pathogenesis of

human rheumatoid arthritis.

• Exhibits severe chronic arthritis of the

forepaws and hind paws, as shown

by gross observation and histological

analysis by approximately 20 weeks

of age.

• A useful model for studying

inflammatory arthritis which

does not require experimental

manipulation.

• Suitable for efficacy testing of

biosimilars.

MODEL NUMBER 1006

NOMENCLATURE


LIGHT PRODUCING TRANSGENIC ANIMALS® (LPTA®)

Reporter models expressing luciferase under the control of various promoters, for use in bioluminescent imaging applications.

β-actin-lucRANDOM TRANSGENIC

• Constitutively expresses luciferase in

all tissues.

• Basal expression of the reporter is highest

in skeletal muscle, thymus, skin, heart,

bone, pancreas, and is detectable in all

tissues, including white blood cells.

• The reporter is constitutively expressed

and is not significantly inducible.

• Useful as a donor animal for studying the

transplantation of various tissue types.

MODEL NUMBER 10498

FVB/NTac-Tg(Actb-luc)46Xen

FVB BACKGROUND

NOMENCLATURE

MODEL NUMBER 10500

B6;FVB-Tyrc-2J Tg(Actb-luc)46Xen

B6;FVB ALBINO BACKGROUND

NOMENCLATURE

• SNP testing has shown that the strain background

is equivalent only to 4-5 backcrosses onto B6 albino,

so the background is designated as B6;FVB.

Gfap-lucRANDOM TRANSGENIC

FVB BACKGROUNDFVB/N-Tg(Gfap-luc)53Xen

• Expresses luciferase under control of

the Gfap promoter, which is inducible

following CNS injury.

• Please inquire for timeline and pricing

for F1 hybrids of the Gfap-luc and B6

albino mice for use in EAE studies.

• As a reporter for neuroinflammation,

luciferase expression provides an

additional readout beyond simple

clinical score in EAE studies with

this model.

MODEL NUMBER 10501

NOMENCLATURE

CRYOPRESERVED CRYOPRESERVED




NFκB-RE-LucRANDOM TRANSGENIC

BALB/c BACKGROUNDBALB/c-Tg(Rela-luc)31Xen

• The model provides for the rapid study

of transcriptional regulation of the

NFκB gene.

• Useful in studying sepsis, arthritis,

inflammatory bowel disease, apoptosis,

tumor development, NFκB gene

regulation, and the treatment of

inflammatory diseases and cancer.

MODEL NUMBER 10499

NOMENCLATURE

ADDITIONAL LPTA® MODELS

AVAILABLE FROM TACONIC’S

CRYOPRESERVED REPOSITORY

MODEL NAME

10627 Gadd45b-luc

10628 Epx-luc

10629 Saa1-luc

10630 IL-2-luc

10646 Cox2-luc

10647 Tnfα-luc

10650 IkBa-luc

• Altered susceptibility to infection, immune

responses and inflammatory responses.

TNF-α KnockoutCONSTITUTIVE KNOCKOUT

C57BL/6 BACKGROUND

LUDWIG INSTITUTE SPONSORED

EMERGING MODEL

MODEL NUMBER 1921

CRYOPRESERVED




EMERGING MODELS

The Emerging Models program includes distribution of investigator-sponsored models which are bred and distributed by Taconic. Since the sponsor sets distribution requirements for each of the Emerging Models, customers may be required to execute an MTA before certain models are delivered.

MODEL NUMBER NOG

MODEL NUMBER 13395

• The CIEA NOG mouse® is a super

immune deficient mouse with

unparalleled potential to engraft human

cells and tissues.

• This severely immunocompromised

mouse carries the scid mutation and a

targeted mutation of the Il2rg gene on

the NOD/ShiJic genetic background.

• The functional knockout of the IL2

receptor common gamma chain

(IL2rg) results in reduction of residual

innate immunity of the NOD/ShiJic

background and superior engraftment

of human cells and tissues compared to

any other immune deficient model.

• Lack of mature T, B, and NK cells,

reduced complement activity, dysfunc-

tional macrophages, and dendritic cells,

and deficiencies in immune signaling,

including impaired cytokine production.

• The polymorphism of SIRP-α allows the

mouse SIRP-α to bind human CD47,

preventing activation of recipient

macrophages to engulf human cells

therefore making it an ideal model for

human immune system engraftment

and PDX.

• Excellent choice for xenograft studies

using cell lines with poor take rates in

nudes or scids, or for engraftment of

patient-derived tumors.

• The best choice for human

immune system engraftment mice,

with successful engraftment of

various tissues such as PBMCs or

hematopoietic stem cells (HSC).

• Test therapeutic antibodies and

immune-modulating treatments

by combining immune system

engraftment of the immune system

with xenograft of tumor cell lines or

patient-derived tumors.

• Displays a very low incidence of

lymphoma, unlike NOD scid model.

• The Il2rg gene is X-linked, so male

knockouts are hemizygous for the Il2rg

mutant allele.

• Sponsored by the Central Institute for

Experimental Animals (CIEA) and

In-Vivo Science International.

• Applications in research involving

cancer, infectious disease (HIV,

malaria), immuno-oncology, CAR-T,

iPS, and humanization immune system

engraftment.

• Now available pre-engrafted with

human hematopoietic stem cell (hHSC)

as huNOG for immediate delivery at

16 weeks post-engraftment.

• NOG mouse expressing human

GM-CSF and IL-3.

• Supports higher overall engraftment

and superior myeloid cell differentiation

after human HSC engraftment.

• Now available pre-engrafted with HSCs

as huNOG-EXL for immediate delivery

at 10 weeks post-engraftment.

• May be a suitable host for human acute

myeloid leukemia (AML) xenografts

that are dependent on human GM-CSF

and human IL-3.

• The polymorphism of SIRP-α allows the

mouse SIRP-α to bind human CD47,

preventing activation of recipient

macrophages to engulf human cells

therefore making it an ideal model for

human immune system engraftment

and PDX.


using cell lines with poor take rates in

nudes or scids, or for engraftment of

patient-derived tumors that requires

human GM-CSF or human IL-3.

CIEA NOG mouse®

NOG-EXL (hGM-CSF/hIL-3 NOG)

T, B & NK CELL DEFICIENT MOUSE

WITH IMPAIRED MYELOID FUNCTIONS

T, B & NK CELL DEFICIENT MOUSE

WITH IMPAIRED MYELOID FUNCTIONS

NOD.Cg-Prkdcscid Il2rgtm1Sug/JicTac

NOD.Cg-Prkdcscid Il2rgtm1Sug Tg(SV40/HTLV-IL3,CSF2)10-7Jic/JicTac

NOMENCLATURE

NOMENCLATURE

NO MTA OR LICENSE FEE IS REQUIRED FOR PURCHASE.



• NOG mouse expressing human IL-2

cytokine.

• Predominant differentiation of human

NK cells following hHSC engraftment.


using cell lines with poor take rates

in nudes or scids, or for engraftment

of patient-derived tumors or tumor

infiltrating lymphocytes that requires

human IL-2.


cytokine.

• Enhanced expansion of human

monocytes following hHSC

engraftment.

• May be a suitable host for human IL-6

dependent tumor such as multiple

myeloma (MM) xenografts.


cytokine.

• Engraftment and expansion of human

NK cells following engraftment with

CD56+ NK cells derived from PBMC.

hIL-2 NOG

hIL-6 NOG

hIL-15 NOG

T, B & NK CELL DEFICIENT MOUSE WITH

IMPAIRED MYELOID FUNCTIONS





NOD.Cg-Prkdcscid Il2rgtm1Sug Tg(CMV-IL6)4-2Jic/JicTac

NOD.Cg-Prkdcscid Il2rgtm1Sug Tg(CMV-IL6)1-1Jic/JicTac

NOD.Cg-Prkdcscid Il2rgtm1Sug Tg(CMV-IL2/IL15)1-1Jic/JicTac

NOMENCLATURE

NOMENCLATURE

NOMENCLATURE

MODEL NUMBER 13440

MODEL NUMBER 13686

MODEL NUMBER 13683

LICENSING: NO MTA OR LICENSE FEE IS REQUIRED FOR ANY OF THE MODELS FEATURED ON THIS PAGE.



CIEA BRG mouseCONSTITUTIVE KNOCKOUT BALB/C BACKGROUND C.Cg-Rag2tm1Fwa Il2rgtm1Sug/JicTac

• Immunodeficient model lacking mature

T, B, and NK cells.

• Higher radiation tolerance due to Rag2

mutation compared to scid models

(similar to wild type mice).

• Model is completely congenic on

BALB/c background, the preferred

strain background for many

immunology studies.

• Applications in studies on immune

system engraftment, infectious

diseases and autoimmune diseases

as well as cancer xenografts.

• Sponsored by the Central Institute

for Experimental Animals and In-Vivo

Science International.

MODEL NUMBER 11503

NOMENCLATURE

%

SP

EC

IFIC

RE

LE

AS

E

SERUM DILUTION

0

20

40

60

80

100

1/641/321/161/81/41/2

0

20

40

60

80

100

1/641/321/161/81/41/2

%

CY

TO

TO

XIC

ITY

E:T RATIO

0

20

40

60

80

100

100:1100:133:1100:1

0

20

40

60

80

100

100:1100:133:1100:1

HEMOLYTIC COMPLEMENT ACTIVITY – NOD scid AND NOG mice

Defect of hemolytic complement activity in sera of NOD scid and NOG mice.

The CIEA NOG mouse® shows defects in complement activity.

NK ACTIVITIES DEFECT – NOG mice

Defect of NK activities in spleen cells of NOG mice.

The CIEA NOG mouse® lacks NK cell activity.

NOGC.B-17 scid NOD scid

NOGC.B-17 scid NOD scid

NO MTA OR LICENSE FEE IS REQUIRED FOR PURCHASE.


IMMUNOLOGY AND INFLAMMATION TACONIC BIOSCIENCES

TACONIC OFFERS

Human Immune System Engrafted ModelsImmunodeficient mice carrying a reconstituted human immune system

The super immunodeficient CIEA NOG mouse® is the ideal model for engraftment of human cells, and therefore the model of choice for combined immune system and tumor engraftment immuno-oncology experiments.

When reconstituted with various human

tissue sources, NOG mice are indispensable

for basic research probing the human

immune system. Engrafted NOG mice enable

efficacy testing of immunotherapies as

well as the unprecedented ability to study

tumor specific modulation of the immune

system. Taconic offers study-ready cohorts of

hematopoietic stem cell-engrafted NOG mice.

In addition to these models, Taconic offers

access to scientific expertise on use of

the CIEA NOG mouse® for engraftment

and reconstitution with human tissues.

For additional information on these models,

visit taconic.com/his-mice

Immune system engrafted mouse models

are excellent tools to evaluate the effect

of human immune cells in preclinical

oncology:

• Assessment of therapeutic

immunomodulatory activities

• Evaluation of antitumor activity

related to antibody dependent

cell cytotoxicity (ADCC)

• Analysis of innate and

adaptive immunity

• Cytokine readouts

These models are also excellent tools

for other research application, such as:

• Immuno-Oncology Research

• GvHD (Graft versus Host Disease)

• T cell activation model

• B cell depletion studies

• Autoimmune disease

• Allergy

• Inflammation

• HIV Research

• Vaccine development

• Transplantation

• Study of hematopoiesis

HOW CAN IMMUNE SYSTEM

ENGRAFTED NOG MICE BE USED?



• Model for investigation of adult/

mature cell populations.

• Use is limited to short term studies.

• GvHD response can be used as a

screening system for T cell

modulating drugs.

• Available with normal or patient-

derived PBMCs.

NOG MICE ENGRAFTED WITH HUMAN

PBMCs (PERIPHERAL BLOOD MONONUCLEAR CELLS)

• Stable engraftment of multiple

cell lineages by 12-16 weeks

post-injection.

• Only mice with ≥25% hCD45+ in

peripheral blood are delivered.

• Long-term studies possible.

huNOG ARE AVAILABLE OFF-THE-SHELF!

PLACE YOUR ORDER NOW FOR

IMMEDIATE DELIVERY.

huNOGNOG MICE ENGRAFTED WITH HUMAN

CD34+ HEMATOPOIETIC STEM CELLS (HSCs)

huPBMC-NOG

LICENSING: NO MTA OR LICENSE FEE IS REQUIRED FOR ANY OF THE MODELS FEATURED ON THIS PAGE.

huNOG-EXL

• Stable engraftment of multiple cell

lineages, with improved myeloid cell

differentiation.

• Only mice with ≥25% hCD45+ in

peripheral blood are delivered.

• Long-term engraftment enables

long-term studies.

NOG-EXL (HGM-CSF/HIL3-NOG) MICE ENGRAFTED WITH

HUMAN CD34+ HEMATOPOIETIC STEM CELLS (HSCs)

huNOG-EXL MICE ARE AVAILABLE

OFF-THE-SHELF! PLACE YOUR ORDER

NOW FOR IMMEDIATE DELIVERY.



COMMON MYELOIDPROGENITOR

IL-1IL-3GM-CSFSCF

COMMON LYMPHOIDPROGENITOR

IL-2IL-7IL-12SDF-1

IL-1 IL-6IL-2 IL-7IL-4

B LYMPHOCYTE TNK PROGENITOR

MYELOBLAST

GM-CSF

BASOPHIL

SCFG-CSFGM-CSFIL-3IL-6

NEUTROPHIL

SCFG-CSFGM-CSFIL-3IL-6

EOSINOPHIL

GM-CSFIL-3IL-5

MONOCYTE

SCFM-CSFG-CSFGM-CSFIL-3IL-6

huNOGhIL-2 NOG, hIL-15 NOGhIL-6 NOGhuNOG-EXL(hGM-CSF/hIL-3 NOG)

HEMATOPOIETICSTEM CELL

T LYMPHOCYTE

IL-2IL-7

NK CELL

IL-15IL-7

HEMATOPOIESIS AND HUMAN IMMUNE SYSTEM ENGRAFTED MICE



CHOOSE TACONIC

For more than 60 years, Taconic has anticipated

the needs of the scientific community to deliver

models and services that meet the diverse needs of

biomedical and biopharmaceutical researchers.

Today that forward thinking and commitment to

working collaboratively has resulted in a client-centric

environment infused with a knowledge bank that

allows you to select the optimum model for your

study based on informed insight into the generation

of genetically engineered mouse and rat models.

YOUR COLLABORATIVE PARTNER

As a full-service biosciences company, Taconic can help

you acquire, test, develop, breed, cryopreserve, prepare,

and distribute highly relevant research lines worldwide.

Whether you require custom genetically engineered,

cell or tissue engrafted models or traditional models,

Taconic’s scientists will partner with you to rapidly

and efficiently deliver the highest quality models.

TALK TO A SCIENTIST

Our scientific teams are happy to meet and talk

with you about the most efficient way to achieve

your study goals. Working in partnership with

clients the world over, our scientific teams offer

expert advice that can help you speed up your

research and reduce your overall costs.

TALK TO A REPRESENTATIVE

For general information, you can talk to a member

of our customer service team. Our customer

service team is here to help you make the right

decisions and get the models you need fast.

Contact us at [email protected]

VISIT TACONIC.COM

For more information on the entire Taconic

portfolio of products and services designed to

help further your research, visit taconic.com

Take Your Research Further

GEMs MANAGEMENT

Taconic’s fully integrated GEMs

Management brings innovative models

from design to study-ready cohorts with

unprecedented speed and transparency.

• Embryology

• Rapid Colony Expansion

• Contract Breeding

• Surgical Services

• Tissue Collection

• Genotyping and Molecular Analysis

• Microbiome and Germ-Free Research

Models and Services

GEMs DESIGN

Taconic Biosciences Genetically

Engineered Models (GEMs) Design

empowers our clients to develop research

models specifically suited to the unique

needs of their discovery and development

studies or therapeutic programs.

• Gene Inactivation

• Gene Mutation or Replacement

• CRISPR Gene Editing

• Transgene Expression

• miRNA Expression

• Cohort Production Packages

PRECISION RESEARCH MODELS

Research organizations demand

precision tools that better reflect human

physiology. Taconic Biosciences leads

the field delivering innovative solutions

to meet these continually evolving

needs. Our core competencies include

the delivery of complex strategies that

both integrate human genetic sequences

and engraft human cells and tissues into

custom mouse and rat models.

• Human Gene Replacement

• Human Cell and Tissue Engraftment

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may not be reproduced in any form without prior permission.

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