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Why use a microscope? To view objects and detail too small to see with human eye. Improves resolution of object List examples of how what you used it for: Blood cell detail rbc, wbc, platelets Bacteria Cocci, rod, bacillus Protozoa Trichomonas Giardia
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Microscope
Important Lab instrument
Why use a microscope? To view objects and detail too small to see with
human eye. Improves resolution of object List examples of how what you used it for:
Blood cell detail rbc, wbc, platelets
Bacteria Cocci, rod, bacillus
Protozoa Trichomonas Giardia
Types of Microscopes Compound microscope
Bacteria, fungi and protozoa
Electron microscopeRequired for viruses
FluorescenceUsed as a diagnostic tool for
immunofluorescence tests
Parts of a microscope Coarse adjustment
1st step in focusing to change the distance between specimen and lens
Fine adjustmentTo fine tune the pictureUsed particularly with 100x and oil objective
StageHolds the slide, moved up and down with
coarse and fine adjustment
Parts of a microscope Objectives
Common objectives are 4x, 10x, 40x, 100x, oilTotal magnification is eye piece magnification
multiplied times objective you are viewing with 40x objective and 10x eye piece is 400x
magnificationOil objective is labeled and is always used
with immersion oil Oil increases the resolving power by focusing the
light rays
Proper care for microscope Always start with lowest objective to focus Always store with lowest objective locked
in place Carry with two hands Cover with dust cover Be sure to clean oil off of oil objective Use fine adjustment with 100x and oil
objective
THE COMPONENTS OF THEMICROSCOPE
What do you remember?
THE MICROSCOPE
EYEPIECE What is it
when there is only 1?
When there are 2?
THE MICROSCOPE
ARM
THE MICROSCOPE
BASE
THE MICROSCOPE
TUBE
THE MICROSCOPE
REVOLVING
NOSEPIECE
THE MICROSCOPE
OBJECTIVELENS
What are the magnification of the 4 objectives? 4, 10, 40, 100x
What magnification is the eye piece? 10x unless otherwise
stated What would be the total
magnification when viewing an object through the 40x objective? 400x
THE MICROSCOPE
MECHANICALSTAGE
Why is a mechanical stage better than using clips?
THE MICROSCOPE
IRIS DIAPHRAGM
What does this do? Control the
amount of light
THE MICROSCOPE
COARSE ADJUSTMENT KNOB
What 2 objectives do you use the coarse adjustment?4 and 10x
THE MICROSCOPE
FINE ADJUSTMENT KNOB
What objectives is this used with?
All, but mostly 40, 100x
THE MICROSCOPE
Light
THE MICROSCOPE
ON/OFFSWITCH
CARE OFTHE MICROSCOPE
Always carry the microscope by the arm and the base and use 2 hands!
CARE OFTHE MICROSCOPE
Wrap the cord up or around the microscope so it is not hanging down.
CARE OFTHE MICROSCOPE
Always lower the stage and clean all objectives off
Always use a dust cover!
