Insecticide Resistance in Southern Punjab

8/6/2019 Insecticide Resistance in Southern Punjab

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University College of Agriculture, B.Z.University, Multan 1

Current Scenario of Insecticide ResistanceCurrent Scenario of Insecticide Resistanceinin

Helicoverpa armigeraHelicoverpa armigera (H(H bner)bner) againstagainst

ConventionalConventionalInsecticidesInsecticides

Umair Faheem,Umair Faheem,Mushtaq A. Saleem, Attaullah K. Pathan, Ghulam M.Mushtaq A. Saleem, Attaullah K. Pathan, Ghulam M.

Cheema and Adeel RehanCheema and Adeel Rehan


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2University College of Agriculture, B.Z.University, Multan

WhyWhy H. armigeraH. armigera ??Commonly known as American bollworm.Commonly known as American bollworm.H. armigeraH. armigera is one the most notorious pestis one the most notorious pestof crops.of crops.Mainly controlled by the application of Mainly controlled by the application of insecticides.insecticides.Application of insecticides has manyApplication of insecticides has manyproblems;problems;

Toxicity to plants and non-target organisms Toxicity to plants and non-target organismsPest resurgencePest resurgenceSecondary pest outbreakSecondary pest outbreakInsecticide resistanceInsecticide resistance


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Resistance inResistance in H. armigeraH. armigera

Insecticide resistance inInsecticide resistance in H. armigeraH. armigera hashasbeen reported throughout the world includingbeen reported throughout the world includingPakistan.Pakistan.Previously high level of resistance has beenPreviously high level of resistance has beenreported in Pakistan against the conventionalreported in Pakistan against the conventionalinsecticides ininsecticides in H. armigeraH. armigera (Ahmad et al.(Ahmad et al.1995, 1997, 1999 and 2001).1995, 1997, 1999 and 2001).

Area under Bt. Cotton and use of newArea under Bt. Cotton and use of newchemistry insecticides is increasing.chemistry insecticides is increasing.


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ExperimentExperiment

Laboratory conditionsLaboratory conditionsStudyStudy areasareas

MultanMultanBahawalpurBahawalpur

D. G. KhanD. G. KhanR. Y. KhanR. Y. Khan

InsecticidesInsecticidesendosulfanendosulfanprofenofosprofenofos

carbosulfancarbosulfandeltamethrindeltamethrin


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Insect RearingInsect Rearing

InsectsInsectsCollectionCollectionRearingRearingFF11 generationgeneration

22 ndnd instarinstar

2 nd instarlarva

Artificialdiet

Pupal

chamber

Nappy liners for egglaying

H. armigera

moths


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Very lowVery low 5-10 fold5-10 foldLowLow 10-20 fold10-20 foldModerateModerate 20-50 fold20-50 foldHighHigh 50-100 fold50-100 foldVery HighVery High 100-above fold100-above fold

Interpretat on o Res stancen erpre a on o es s anceLevelsLevels


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ompar son oompar son oSusceptibilitySusceptibility

Comparison of Susceptibility of Lab-PK strain of Helicoverpa armigera against Different Insecticides

4.40

23.30

14.21

5.30 4.42

17.66

6.13 6.12

0.00

5.00

10.00

15.00

20.00

25.00

30.00

35.00

endosulfan profenofos carbosulfan deltamethrin

L C

5 0

Leaf dip

Topical

Most ToxicInsecticides


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Res stance aga nstes s ance aga nsendosulfanendosulfan

0

200

400

600

800

1000

1200

1400

16001800

Lab-PK D.G.Khan Bahawalpur R.Y.Khan Multan

L C

5 0

Leaf dip

Topical

Comparative studies on LC 50 values of endosulfan by two bioassay methods

against Helicoverpa armigera


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es s ance aga nses s ance aga nscarbosulfancarbosulfan

0

100

200

300

400

500

600

700

800

900


L C

5 0

Leaf dip

Topical

Comparative studies on LC 50 values of carbosulfan by two bioassay methods



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Res stance aga nstes s ance aga nsdeltamethrindeltamethrin

0

200

400

600

800

1000

1200


L C

5 0

Leaf dip

Topical

Comparative studies on LC 50 values of deltamethrin by two bioassay methods



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Resistance RatioResistance RatioLeaf dipLeaf dip TopicalTopical

endosulfanendosulfan D.G. KhanD.G. Khan 2323 1212BahawalpurBahawalpur 0202 0505R.Y. KhanR.Y. Khan 2727 3636MultanMultan 0505 1616

profenofosprofenofos D.G. KhanD.G. Khan 1313 6565

BahawalpurBahawalpur 0202 0202R.Y. KhanR.Y. Khan 0707 0909MultanMultan 0707 3232

carbosulfancarbosulfan D.G. KhanD.G. Khan 1212 2727BahawalpurBahawalpur 0909 3333R.Y. KhanR.Y. Khan 6464 105105MultanMultan 0606 1919

deltamethrindeltamethrin D.G. KhanD.G. Khan 108108 3535BahawalpurBahawalpur 0707 2121R.Y. KhanR.Y. Khan 2929 3333MultanMultan 6565 1313

ompar son o es s anceompar son o es s anceRatiosRatios


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endosulfanendosulfan profenofosprofenofoscarbosulfancarbosulfan

Leaf dipLeaf dip TopicalTopical Leaf dipLeaf dip TopicalTopical Leaf dipLeaf dip TopicalTopical

profenofosprofenofos 0.760.76 0.050.05

0.060.06 nsns

carbosulfancarbosulfan 0.740.74 0.050.05 0.920.92 0.010.01 0.190.19 nsns -0.16-0.16 nsns

deltamethrindeltamethrin 0.540.54 0.050.05 0.910.91 0.050.05 0.960.96 0.010.01 0.510.51 0.050.05 -0.08-0.08 nsns 0.670.67 0.050.05

Cross ResistanceCross Resistance


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ConclusionConclusion

Insecticide resistance has mostly prevailed at low toInsecticide resistance has mostly prevailed at low tomoderate levels.moderate levels.Avoid spray of carbosulfan at R.Y. Khan andAvoid spray of carbosulfan at R.Y. Khan anddeltamethrin at D.G. Khan to controldeltamethrin at D.G. Khan to control H. armigeraH. armigera ..Multiple resistance was observed in most of the strains.Multiple resistance was observed in most of the strains.No significant difference was observed between theNo significant difference was observed between thetopical and leaf dip bioassay methodtopical and leaf dip bioassay methodRotational use of new chemistry and conventionalRotational use of new chemistry and conventionalinsecticides.insecticides.


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AcknowledgementAcknowledgement

Thanks to Pakistan Agricultural Thanks to Pakistan AgriculturalResearch Council for providingResearch Council for providingfunding and facilities to conduct thisfunding and facilities to conduct thisresearch.research.


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Documents

Insecticide Resistance in Southern Punjab