Introduction to Benthic Macroinvertebrates

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Introduction to Benthic Macroinvertebrates. Trout In The Classroom Virginia Council of Trout Unlimited 2009 / 2010. State Water Quality Standards. Standards are based on how we use water: 1. 2. 3. - PowerPoint PPT Presentation

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  • Introduction to Benthic MacroinvertebratesTrout In The ClassroomVirginia Council of Trout Unlimited2009 / 2010

  • State Water Quality StandardsStandards are based on how we use water:1.2.3.Standards include a general requirement that all state waters are free of pollutants harmful to animal, plant, and aquatic life. This standard allows the state to consider stream habitat and aquatic insects as indicators of water quality.

  • Aquatic Insects as Bio-Indicators of Water QualityThere are important reasons why benthic macroinvertebrates are used to assess water quality: 1) they are found in all streams;2) there are many different species;3) they stay in the same spot in a stream;4) they react to different conditions in the stream over long periods of time; and5) they are easy to sample and identify.

  • Benthic MacroinvertebratesBenthic = dwelling on stream bottomMacro = large enough to see with naked eyeInvertebrates = without a backboneOther benthic aquatic organisms = aquatic insects that have three body parts (head, thorax, abdominal segment) and six legs

    When you try to identify an organism, these are some details to notice

  • Key ID Features -LegsSix true legs plus lateral filamentsSix segmented legsMore than six legsprolegs

  • Key ID Feature TailsTwo tailsFeather-like tailsNo tail or tiny tailTail-like projection

  • Details to notice: gillsAbdominal gillsAbdominal filamentous gillsInternal gills

  • Practice IDBody shape?Head? Size relative to body?Legs? Number? Length? Placement?Details? Antennae? Eyes? Wings? Tail?Wing pads

  • Specimen #1

  • Specimen #2

  • Specimen #3

  • Specimen #4

  • Specimen #5

  • Specimen #6

  • Specimen #7

  • Specimen #8

  • Specimen #9

  • Specimen #10

  • Specimen #11

  • Specimen #12

  • Specimen #13

  • Specimen #14

  • Specimen #15

  • Benthic Macroinvertebrates can be classified according to their sensitivity to pollutants.

  • SensitiveMayfly larvaeStone Fly larvaeMost caddisfly larvaeBeetles (adults and larvae)

  • Somewhat sensitiveDragonfly and damselfly larvaeNetspinner caddisfly larvaeCrayfishGilled snailsAquatic sowbugsScudsClams and musselsTrue fly larvaeHellgrammites, Fishfly and Alderfly larvae

  • TolerantLunged snailsBlack fly larvaeMidge larvaeAquatic wormsFlatwormsLeeches

  • Interpreting Benthic DataBenthic macroinvertebrates can be classified according to their sensitivity and tolerance to water pollution.

  • Identify and Tally

  • Individual Species Metric

  • Tolerance Metric

  • Compute Mulitmetric Index

  • Rate the Stream

  • CreditsSlides and notes used in this presentation were provided for educational purposes by:Virginia Save Our StreamsVirginia Department of Conservation and RecreationAlice Furguson Foundation Bridging the WatershedAudubon Naturalist SocietyAnd a very special thanks to Darrell Schwalm of the Shenandoah Valley TU chapter for putting this presentation together.

    The suitability of a stream for brook trout depends, in part, on the availability of food. This is a presentation about fish food how to identify different types of fish food and how fish food can indicate whether a stream is healthy enough for trout to live.

    Lets start our discussion by thinking about how we (people) use water in our streams and rivers. [Brainstorm with students about how we use water and organize their responses into three general categories drinking water uses, recreational uses, and aquatic life uses.]

    Our states and the Federal government have laws that protect our streams, rives, and lakes for these uses.

    State water quality standards are based on three general types of uses:Drinking waterRecreation uses including swimmingAquatic life (see text on slide)

    Which standard is the most stringent?The suitability of a stream, river, or lake for drinking water and recreational use depends primarily on its bacteria and chemical quality.

    However, the suitability of a stream for aquatic life is based primarily on the availability of fish food. This slide tells why aquatic insects are good indicators of water quality.The more scientific term for fish food is benthic macroinvertebrate or macros.Benthic = dwelling on the stream bottomMacro = large enough to see with the naked eye although a low power field microscope is helpful to distinguish details.Invertebrate = without a backbone. Most benthic macroinvertebrates are aquatic insects that have three body parts (head, thorax, abdominal segment) and six legsOther = other invertebrate animals that live on the stream bottom include worms, clams, mussels, and crayfish. Macros are an important link in the food web between the producers (leaves, algae, etc.) and the higher consumers such as fish.

    [Have the students use their copy of the VASOS ID sheet as you go through the different organisms quickly.]

    There are three key features of macroinvertebrates that help identify the different animals and species.

    The first feature are legs are legs present, how many, and what type? Be careful because some organisms have structures that may look like legs such as the lateral filaments used for breathing in the insect in the slide.The second key feature are tails is there a tail, and if so, how many parts does it have and what is the shape of the parts?The third key feature are gills are gills present, and if so, where are they located on the insect and what are their shape? Note that there are organisms like some snails that have internal gills that you can not see.There are some other secondary factors that also help distinguish one species of macroinvertebrates from another. The practice specimen to identify shows some of these other factors: Body shape; Head shape and size; Wingpads; Placementg of legs; and Antennae

    The practice specimen is a dragon fly nymphCrayfish -Note lobster-like in appearance, 5 pairs of legs, 1st pair modified into large pinchers, head & thorax combined (cephalothorax), and can be quite smallMayfly nymph: note three tails (some species have two tails), abdominal gills visible, and antennaeStonefly nymph: note long antenna, two tails, no abdominal gills, distinctive separation of the wing pads on the thoraxLeech: note the segmentation and absence of head or tailCaddisflies - Order Trichoptera elongate, generally caterpillar-like in appearance (closely related to butterflies & moths)3 distinct body parts visible -distinct head w/ small, simple eyes & usu. inconspicuous antennae3 pairs of true, jointed legshave cases made of gravel, sand, or organic matter covering their abdomen (case-making forms often collected w/o case)complete metamorphosis (no wing pads)Clams, Mussels, & Snails (Phylum Mollusca)hardened shell covering soft body (for fresh water species in our area)Clams & Mussels - Class Pelecypodatwo opposing hinged shellsconcentric growth rings on shellsvaried colorscan be mistaken for pebblesDragon & Damselflies - Order Odonatametamorphosis is incompleteeither robust or elongate body forms w/ distinct segmentshead distinct w/ relatively large, well developed eyeswing pads present in later instarsprehensile labium (lower lip)3 pairs of true, jointed legsvaried sensitivity

    Damselflies (Zygoptera) - more delicate, thin, cylindrical body form than dragonfly- three paddle-like gills (caudal lamellae) protrude from rectum- relatively long, thin legs note three tails that are featherlike and broader than a mayfly tail, long legs, long-slender abdomen, protruding eyes on side of headArthropods (Phylum Arthropoda)jointed legs (adults, not always in immature)distinct body segmentsexoskeleton (must shed to grow)Crustaceans - Class Crustaceasegmented bodies2 pair of antennae

    Scud - Order Amphipodalaterally (side-to-side) flattenedGenerally curled, shrimp-like in appearanceoften swim with a sideways motion, good swimmersfemales sometimes w/ eggsmore than 6 legsCaddisflies - Order Trichoptera elongate, generally caterpillar-like in appearance (closely related to butterflies & moths)3 distinct body parts visible -distinct head w/ small, simple eyes & usu. inconspicuous antennae3 pairs of true, jointed legsusu. one or more thoracic plates (but abdomen not sclerotized)anal prolegs with a single hook on each (closely appressed to body in case-making forms)some have filamentous abdominal gills

    Net-spinning caddisflies. Note the six legs, three dorsal plates, and filamentous gills on the underside.Flies - Order Dipteragenerally maggot-like in appearance, often w/o immediately distinguishable body partsmay or may not have distinct/visible headhead, if visible, has simple eyes and inconspicuous or no antennaeno true, jointed legs and no wing padsmay have prolegs on thorax or abdomen-abdomen may end in proleg(s) or other terminal processmetamorphosis is completesome swim w/ jerking motion, others attach to substrate (or collecting pan), and others crawl on bottom

    Crane fly, a True Fly larva. Usually quite large, with tail-like projections.Dragon & Damselflies - Order Odonatametamorphosis is incompleteeither robust or elongate body forms w/ distinct segmentshead distinct w/ relatively large, well developed eyeswing pads present in later instarsprehensile labium (lower lip)3 pairs of true, jointed legsvaried sensitivity

    Dragonflies (Anisoptera) - robust bodies from cylindrical to dorsoventrally flattened- abdomen broadens from base- especially large eyes- gills in rectal chamber (no external gills); 3 anal structures- can move by "jet propulsion"

    Dragonfly larva: note the tiny three tails and variable body shapes.Beetles - Coleopteravery diverse, challenging groupmetamorphosis is complete- some adults are aquatic (elytra)

    For Larvae- body generally elongate, often sclerotized (hardened)- head usu. sclerotized & well defined, some w/ well developed mandibles- usu. have 3 pairs of true, jointed legsno wing padsabdomen may have lateral or terminal filaments

    Shown in slide -- Left is an adult water boatman. Top is a water penny larva, and bottom is a riffle beetle larva. Clams, Mussels, & Snails (Phylum Mollusca)hardened shell covering soft body (for fresh water species in our area)Clams & Mussels - Class Pelecypodatwo opposing hinged shellsconcentric growth rings on shellsvaried colorscan be mistaken for pebblesSnails - Class Gastropodasingle coiled or spiral shellextend fleshy "foot" for locomotion

    Lunged snails, with opening on the left. Flat coiled snails are also lunged snails.Flies - Order Dipteragenerally maggot-like in appearance, often w/o immediately distinguishable body partsmay or may not have distinct/visible headhead, if visible, has simple eyes and inconspicuous or no antennaeno true, jointed legs and no wing padsmay have prolegs on thorax or abdomen-abdomen may end in proleg(s) or other terminal processmetamorphosis is complete

    True Fly Larva:Top Black fly larva. Very small, with bulge at one end where it has a suction cup, with which it attaches itself to rocks, and then waves the projections at the other end to catch food.Bottom Midge larva a tiny head, two pair of prolegs near the head and at the tail endHellgrammites, Fishflies, & Alderflies - Order Megalopteraelongate, somewhat dorsoventrally flattened forms that can be quite large (3-4") - distinct heads w/ well developed jaws- 3 pairs of true, jointed legs and no wing pads - abdomen has lateral filamnts (don't mistake for legs) - abdominal gill tufts present in some forms (hellgrammite)- abdomen ends either in either 2 prolegs, each w/ 1 pair of hooks, or in a single, often brushy filament (latter in alderfly)- may have 2 breathing tubes near end of abdomen (fishfly)- metamorphoses is complete

    Fish fly larva. Note the lateral filaments, or fake legs. Sometimes very large (up to 4 inches) with sharp biting jaws.Stream-bottom macroinvertebrates differ in their sensitivity to water pollution. Some stream-bottom macroinvertebrates cannot survive in polluted water. Others can survive or even thrive in polluted water. In a healthy stream, the stream-bottom community will include a variety of pollution-sensitive macroinvertebrates. In an unhealthy stream, there may be only a few types of nonsensitve macroinvertebrates present. Macros that are sensitive to pollution will be found in healthy streams that are little impacted by human activities. These streams will normally have a tree-lined riparian buffer that provides shade to keep the water cool in the summer, leaves that provide food for aquatic insects, and tree roots that hold the soil along the stream banks and prevent erosion.

    The stream water will normally be cool in the summer to hold high levels of oxygen. The stream bed or substrate will include many riffle areas with cobble size stones that provide abundant living spaces for a variety of insects. The stream bottom will also be relatively free of sediments that could fill the spaces between stones and smother the insects.

    Finding pollution sensitive aquatic insects is a good indication that the stream is very suitable for trout.Somewhat sensitive macros are found in streams that are moderately impacted by human activities. These streams have riparian buffers that are only partially forested so there are sections where stream banks are eroding and moderate amounts of sediments are entering the stream. The water temperatures are warmer in the summer because there is less shade, and dissolved oxygen levels are lower than good quality streams.The streams have fewer riffle areas, and the riffles are normally composed of gravel size stones, sands and other fine sediments that fill some of the living spaces.

    The upper reaches of these streams that are least impacted by human activity, and lower reaches that receive cold spring water may support a trout population if the water temperatures are cool enough in the summer to maintain sufficient dissolved oxygen. Macros tolerant to pollution live in poor quality streams that are highly impacted by human activity. The riparian buffers are normally without trees and stream-bank erosion is common. The stream bottom has a lot of sediment that limits living spaces for the aquatic insects. Water temperatures are warm in the summer and dissolved oxygen levels are low at times.

    Finding a high percentages of pollution tolerant macros is an indication that the stream will not support a trout population.All streams are impacted by human activity. Therefore, it is the degree of impact that is important to determine whether a stream is suitable for trout. Little impacted streams are good and highly impacted streams are bad. These good and bad streams can often be identify just by looking at them and visually assessing that environmental factors that indicate a good quality stream forested riparian buffers, little stream-bank erosion, cobble riffles, and little sediment on the stream bottom.

    Most streams and sections of streams fall in the middle they have good quality sections and poor quality sections. These streams are harder to judge. Studies conducted by environmental scientists have been used to create measurements or metrics that will indicate the relative health of a stream. There are many metrics that are helpful, and the best measurements use a variety of metric, or multimetrics, together to assess the quality of a stream.

    Applying metrics to assess the quality of a stream is based on the assumption that the samples collected to calculate the metrics follow the same accepted standard procedures used by the scientist that did the studies.To apply metrics that indicate stream health, the macros first need to be identified to the order level, and in some cases, to the family level. The tally sheet is used to record and organize the results.

    There should be at least 200 individual macros identified in the sample to have a sample that is representative of actual conditions in the stream. A sample with a small number of macros can provide misleading results.The diversity of organisms in the sample and the percentage of each organism in the sample are two important measures used to assess stream health. Having a high diversity indicates that the stream has good quality riffles, fast and slow moving waters, and sections with shallow and deeper water levels. Healthy streams will have several macro species that are sensitive and moderately sensitive to pollution. Each macro species has been given a tolerance level based on scientific studies. Calculating the tolerance metric indicates the relative abundance of pollution sensitive species.

    The macros that are most sensitive to pollution have the lowest tolerance number zero is best. The tolerance scale goes up to 10 so 10 is bad. Therefore, the low tolerance metric scores indicate best quality streams.Combining the scores of the different metrics provides the best indication of the health of a stream. The multimetric index is designed so the higher the score, the better quality the stream.The multimetric index can be used to characterize the quality of a stream. The VASOS rating system presented in this slide is not used specifically to assess the suitability of streams for trout. Rather, it is used to assess whether a stream is likely to meet the VA state water quality standards for aquatic life. Many streams that meet this aquatic life standard will not support a trout population. Therefore, the streams most likely to support trout are those with the higher scores, and the streams most unlikely to support trout have the lower scores.