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Introduction to Maize Breeding
Nathan Schnur
Overview
What is Plant Breeding? Basic Genetics Mendelian Genetics Principles of Breeding Maize Plant Breeding Methods
Plant Breeding
What is it? Is the science and art of improving crop plants through the
study and application of genetics, agronomy, statistics, plant pathology, entomology, and other related sciences.
Goals Nutritional enhancement, yield improvement, environmental
stress tolerance, improved plant structure, or pest and disease resistance
Basic Plant Genetics
DNA Primary carries of
heritable information Composed of thousands
of genes Genes
Contain actual heritable information
There can be many versions of genes called alleles
Basic Plant Genetics Cont’
There are two alleles for any gene Dominant and Recessive
Two dominant alleles are denoted as “AA” and called homozygous dominant
Two recessive alleles are denoted as “aa” and called homozygous recessive
If one dominant and one recessive allele is present then it is denoted as “Aa” and called heterozygous.
Basic Plant Genetics Cont’
Alleles which are heterozygous or homozygous affect the phenotypes and genotypes of plants
Phenotype Visual appearance of the plant
Genotype Genetic makeup of the plant
Mendelian Genetics
Gregor Mendel Famous geneticist from 1800’s His research consisted of selectively
breeding garden peas He developed two important laws of
genetics Law of Segregation Law of Independent Assortment
Mendelian Genetics Example
Principles of Breeding Maize
Three Main Principles Inbreeding Hybridization Heterosis
Inbreeding
Main Goals Increase the homozygosity at all or specific loci
in the plant genome Produce a plant which breeds true Produce uniform plants
Hybridization of Maize
Hybridization occurs when inbred parents are mated (cross pollinated)
Creates a heterozygous individual Benefits
Increased heterosis (vigor) in F1 generation
Heterosis (Hybrid Vigor)
Heterosis occurs when two homozygous individuals are cross pollinated. This causes all loci to become heterozygous The increased heterozygosity causes
increased plant vigor Benefits of Increased Vigor
Increased yield Better standability Better germination Overall better plant performance
Maize Breeding Methods
Main Methods of Maize Breeding Selfing (Inbreeding) Sib Mating Crossing Test Crossing Backcrossing
Selfing
Selfing is the process of pollinating a plant with its own pollen
Benefits Increased homozygosity Plants which breed true from
generation to generation Decreased Segregation
Disadvantages Many generations of selfing
lead to inbreeding depression
Crossing and Test Crossing
Crossing is useful when trying to create hybrid seed. Ex. By crossing “Inbred A x Inbred a” you
would obtain an F1 hybrid Aa Crossing is used to produce the hybrid
seed farmers use to plant in the spring Test Crossing is useful to test general
combining ability of an individual Ex. Inbred A is x to a tester which has a
diverse selection of genotypes
Backcrossing
Backcrossing is a method which is used to improve a trait which a plant is deficient in.
Method A hybrid plant which has the
trait of interest is crossed with one of its parents
The offspring are then crossed back to the parent, thus increasing the frequency of the trait.
Conclusion
Plant breeding is a complicated but beneficial process.
There are many processes involved which are used in the development of new varieties.
Remember if it wasn’t for plant breeding we wouldn’t have such high yielding crops
References
Fehr, W. Principles of Cultivar Development. 1993. Volume 1
Photos Courtesy of www.pioneer.com www.britannica.com www.psu.edu http://imbgl.cropsci.uiuc.edu www.nsf.gov www.scienceclarified.com
Questions
Questions?