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Glutathione Peroxidase 3
Kadiatu Kamara
Research Summary
• This research seeks to understand the relationship between chronic kidney disease and low levels of glutathione peroxidase 3. We hypothesized that patients with certain stage of chronic kidney disease witnessing a deficiency in glutathione peroxidase 3 (GPx-3) face a chance of uremic cardiomyopathy. With this research we showed:
I. The function and role of the protein GPx-3;II. Experimental data on western blots, chronical studies on mouse,
GPx-3 Elise to understand the protein; andIII. Further studies and limitations.
GPx-3Also known as extracellular glutathione peroxidase is a antioxidant that is made in the basolateral compartment of the kidney. It is transported to the systemic circulation with functions in the plasma and on circulating platelets.
Methods
• Western blot to identify multiple circulating proteins.• Mouse studies to isolate the
exclusive effect of GPx-3.• GPX-3 (human) Elisa Kit used for in
vitro quantitative determination.
Cystatin
Complement Factor D
Protein 6
Retinol binding protein
GPx-3L - Selectin
What does this mean for the future?!
Thank You