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LABORATORY EXERCISES FOR MALE REPRODUCTIVE SYSTEM
Slide #101 (1096). Testis, rat. sustentacular (“Sertoli”) cells pale nuclei of these cells are visible adjacent to the limiting membrane of the seminiferous tubules Nuclei of Sustentacular cells
THE SERTOLI CELLS (SUSTENTACULAR CELLS) EXTEND THE FULL THICKNESS OF THE WALL OF THE SEMINIFEROUS TUBULE. IN OTHER WORDS, THE SERTOLI CELLS EXTEND FROM THE BASEMENT MEMBRANE TO THE LUMEN OF THE SEMINIFEROUS TUBULE. THE FUNCTION OF THE SERTOLI CELL IS SUPPORT FOR THE DEVELOPING GERM CELLS.
Leydig cells
Spermatogonia Spermatocytes Spermatids
http://viewer.serenusview.com/Viewer.aspx?SlideId=96cfb845-a9be-4ac2-ad3c-928070b369ad
Horse Sperm This EM at http://peer.tamu.edu/toolkit2.asp
Slide #101 (1096). Testis, rat. Nuclei of sustentacular or Sertoli cells
Leydig cells
Spermatogonia Spermatocytes Spermatids
seminiferous tubule lumen
Myoid cell nucleus
Spermatocyte
http://viewer.serenusview.com/Viewer.aspx?SlideId=96cfb845-a9be-4ac2-ad3c-928070b369ad
Slide #101 (1096). Testis, rat.
Sustentacular cell nuclei or Sertoli cells Leydig
cells
Spermatocytes
seminiferous tubule
Myoid cell WHEN A SPERMATOGOIUM CELL IS SIGNALED TO UNDERGO MEIOSIS INSTEAD OF MITOSIS, THE CELL HAS REPLICATED ITS DNA TO BECOME 4N AND HAS ENTERED INTO THE FIRST PHASE OF MEIOSIS BUT NOT YET DIVIDED, SO THAT IT IS A DIPLOID 4N CELL, WHICH IS CALLED A PRIMARY (1°) SPERMATOCYTE.
Secondary spermatocytes
Meiotic figures (metaphase plates)
spermatids
Spermatogonia
Primary S. Secondary S. spermatid
http://viewer.serenusview.com/Viewer.aspx?SlideId=96cfb845-a9be-4ac2-ad3c-928070b369ad
Meiosis Mitosis
Human testis Epon toluidine blue stain 19680 http://viewer.serenusview.com/LinkHandler.axd?LinkId=d51e0ced-5512-4721-96c4-f9239ff75af7
Mitotic metaphase
Meiotic metaphase I
Secondary spermatocytes
base
lumen
Mitotic metaphase
Meiotic Metaphase I
Meiotic Metaphase II
http://viewer.serenusview.com/LinkHandler.axd?LinkId=d51e0ced-5512-4721-96c4-f9239ff75af7http://viewer.serenusview.com/LinkHandler.axd?LinkId=d51e0ced-5512-4721-96c4-f9239ff75af7
SEMINIFEROUS TUBULES COMPOSED OF:
SERTOLI CELLS MYOID CELL nucleus
GERM CELLS
SPERMATOGONIA, SPERMATOCYTES, SPERMATIDS Human testis
Primary SPERMATOCYTES SPERMATIDS
human
Slide #107 (F3- 405-172a). Testis, epididymis, ductus deferens (tail of epididymis), cat.
seminiferous tubules
epididymis
Testis
ductus deferens (tail of epididymis)
rete testis and mediastinal testis
interstitial tissue between the seminiferous tubules is quite thin.
Leydig cells produce TESTOSTERONE
http://viewer.serenusview.com/Viewer.aspx?SlideId=f7b6c8c0-d209-4159-803a-3a2d3e6b02c6
Slide #107 (F3- 405-172a). Testis, epididymis, ductus deferens (tail of epididymis), cat.
The interstitial tissue between the seminiferous tubules is quite thin
Leydig cells produce TESTOSTERONE
CCT capsule’s proper TUNICA ALBUGINIA
http://viewer.serenusview.com/Viewer.aspx?SlideId=f7b6c8c0-d209-4159-803a-3a2d3e6b02c6
Slide #107 (F3- 405-172a). Testis, epididymis, ductus deferens (tail of epididymis), cat.
Sertoli cells; spermatogonia; primary spermatocytes; and early and late spermatids
Secondary spermatocytes
spermatogonia;
http://viewer.serenusview.com/Viewer.aspx?SlideId=f7b6c8c0-d209-4159-803a-3a2d3e6b02c6
Golgi phase
Cap phase
Maturation phase
Elongation phase
DEMO SLIDE BOX 100 – Testis, stallion.
sustentacular (“Sertoli”) cells;
LEYDIG (INTERSTITIAL) CELLS ARE LARGE, ROUND TO OVAL CELLS WITH ACIDOPHILIC BUT OFTEN PALELY STAINING CYTOPLASM. SOMETIMES THESE CELLS CAN APPEAR FOAMY DUE TO THE FACT THAT THEY ARE STEROID PRODUCING CELLS.
Sertoli cells; spermatogonia; primary spermatocytes; late spermatids
Leydig cells
http://viewer.serenusview.com/Viewer.aspx?SlideId=6e3fdd19-5e68-415a-9369-6624527a4216
DEMO SLIDE BOX 99 –Testis, newborn kitten.
Nuclei of sustentacular cells
gonocytes
rete testis and mediastinal testis
epididymis
http://viewer.serenusview.com/Viewer.aspx?SlideId=459bba7b-4b40-419b-a4bf-c8dd56331026
DEMO SLIDE BOX 226 – (PM-172 b). Testis, epididymis, ductus deferens, pig (immature).
gonocytes
epidermidis rete testis and mediastinal testis
Dividine gonocytes
Nuclei of sustentacular (“Sertoli”) cells;
http://viewer.serenusview.com/Viewer.aspx?SlideId=74891c6c-cbdf-4c23-ae80-ffaaab695f3e
DEMO SLIDE BOX 187 (935). Testis, boar.
Cryptorchid testis
Slide #107 Normal cat testis
DEGENERATION and loss of germ cells in the seminiferous tubules; however, it illustrates the open spaces between tall Sertoli cells where germ cells would have been located as indicated in the drawing of Sertoli cells
IF THE MALE IS A BILATERAL CRYPTORCHID THEN HE WILL BE INFERTILE; IF THE MALE IS A UNILATERAL CRYPTORCHID THEN HE likely WILL HAVE REDUCED FERTILITY.
http://viewer.serenusview.com/Viewer.aspx?SlideId=afdbce42-4830-43ef-99ab-9eafb7dd2a66http://viewer.serenusview.com/Viewer.aspx?SlideId=f7b6c8c0-d209-4159-803a-3a2d3e6b02c6
DEMO SLIDE BOX 189 (985) -Epididymis, goat.
ductuli aberrantes vestigial mesonephric tubules
ductuli efferentes.
ductus epididymidis.
http://viewer.serenusview.com/Viewer.aspx?SlideId=ab6a0aee-ba50-4782-bc03-3b48424fbd38
DEMO SLIDE BOX 224 – (641). Epididymis, boar.
ductus epididymidis,
ductuli efferentes
http://viewer.serenusview.com/Viewer.aspx?SlideId=0694ed92-055e-4b38-83f9-195c9e03dfd1
DEMO SLIDE BOX 101 –Ductus deferens, stallion.
thick tunica muscularis
smooth muscle
epithelium and lamina propria/submucosa
http://viewer.serenusview.com/Viewer.aspx?SlideId=746133dd-9da4-445c-a853-f7294e2929da
Slide #119 (Ed 904-160c). Ampulla, donkey.
simple or stratified cuboidal epithelium
proteinaceous material In lumen of the ampulla.
CCT and smooth muscle.
http://viewer.serenusview.com/Viewer.aspx?SlideId=ab5b8e54-ccfc-40d1-8cbb-dc2d8b539b06
DEMO SLIDE BOX 102 – (998.) Ampulla of ductus deferens, stallion. simple or stratified cuboidal epithelium
Smooth muscle
http://viewer.serenusview.com/Viewer.aspx?SlideId=d208f1d6-0434-4dbc-97e1-35b5c9c410c6
Slide #126 (Dog 1-160-1). Prostate, dog. urethral lumen. TRANSITIONAL
simple cuboidal or simple columnar (like the prostate) and doesn’t have stereocilia.
http://viewer.serenusview.com/Viewer.aspx?SlideId=bd6bd2ad-d2ca-4939-9a10-39e842fc4e74
DEMO SLIDE BOX 105 – Prostate gland, dog.
Urethra with transitional epithelium
fibromuscular tissue
small ampullae of the ductus deferens
http://viewer.serenusview.com/Viewer.aspx?SlideId=6e9cb3c5-35de-4c2d-ac4e-2a0504d25537
DEMO SLIDE BOX 103 – Prostate gland (pars disseminata), bull
SKELETAL MUSCLE, WHICH IS CALLED THE URETHRALIS MUSCLE OR THE EXTERNAL URETHRAL SPHINCTER
principal duct of the vesicular glands or ductus deferens;
urethra
http://viewer.serenusview.com/Viewer.aspx?SlideId=a20a2c23-6b61-4642-803c-237e088f3974
Slide #131 (946) -- Bulbourethral gland, boar.
http://viewer.serenusview.com/Viewer.aspx?SlideId=d932457e-8abe-48ed-bb86-85605fa65535
Slide #137 (Ed 904-173B). Vesicular gland, stallion.
vesicular glands or seminal vesicles
seminal vesicles because they are hollow, blind sacs
http://viewer.serenusview.com/Viewer.aspx?SlideId=29e62488-88fd-4640-92e6-e928de707826
DEMO SLIDE BOX 191–(991) Bulbourethral gland, goat.
skeletal muscle fibers of the bulbospongiosus muscle.
mucous character
http://viewer.serenusview.com/Viewer.aspx?SlideId=c11d8747-b9fd-4b30-844c-10940cceb3b4
DEMO SLIDE BOXES 107– Penis, dog.
tunica albuginea (of the penis CCT)
pectiniform septum - separates the paired corpora cavernosum
urethra -TRANSITIONAL
PRIMARILY SMOOTH MUSCLE ( YOU MAY SEE A FEW SKELETAL MUSCL FIBERS MIXED IN WITH THE SMOOTH MUSCLE – ESPECIALLY PROXIMALLY
dorsal penile artery, vein & nerve- MUSCULAR ARTERY
http://viewer.serenusview.com/Viewer.aspx?SlideId=52ed2528-7333-402f-907e-df394b944670
DEMO SLIDE BOXES 108 – Penis, dog.
http://viewer.serenusview.com/Viewer.aspx?SlideId=5dd34b00-c3ce-48bf-91e7-12883a8baaca
DEMO SLIDE 193 –(1086). Penis, dog.
internal lamina of the prepuce preputial cavity skin of the penis pars longa glandis - peripherally located erectile tissue urethra corpus spongiosum - erectile tissue surrounding the urethra os penis
the glans penis and includes part of the prepuce
http://viewer.serenusview.com/Viewer.aspx?SlideId=4b1dd44f-07ee-4941-b65b-a78e7fa85180
DEMO SLIDE BOX 192 (1054). –Penis, bull calf.
corpus cavernosum penis, urethra, corpus spongiosum.
internal lamina of the prepuce are fused at this location
skin of the penis and
http://viewer.serenusview.com/Viewer.aspx?SlideId=09cda2c9-732b-4386-9b5d-4b156648faa8
Slide #56 (Pm1-166C-B1). Penis, boar (immature).
nerve, collagenous connective tissue (CCT), large vein, arterioles, venules, capillaries.
corpus cavernosum penis, the urethra, and the corpus spongiosum.
nerve
Unilocular adipose Tissue (white fat),
Small muscular artery,
http://viewer.serenusview.com/Viewer.aspx?SlideId=b001592c-4e49-43ea-92f2-5b7d36b91aa1
Human Penis – transitional epithelium and surrounding spongy cavernous of penal urethra
277
corpus cavernosum penis
urethra
Corpus spongiosum
Tunica albuginea (of the penis, CCT)
Smooth muscle
nerve
Transitional epithelium of urethra
Corpus spongiosum skin
http://viewer.serenusview.com/Viewer.aspx?SlideId=73ea92e8-6b30-41ea-ad85-fd6bd91bb962
Slide Number 1Slide Number 2Slide Number 3Slide Number 4Slide Number 5Slide Number 6SEMINIFEROUS TUBULES COMPOSED OF: Slide Number 8Slide Number 9Slide Number 10Slide Number 11Slide Number 12Slide Number 13Slide Number 14Slide Number 15Slide Number 16Slide Number 17Slide Number 18Slide Number 19Slide Number 20Slide Number 21Slide Number 22Slide Number 23Slide Number 24Slide Number 25Slide Number 26Slide Number 27Slide Number 28Slide Number 29Slide Number 30Slide Number 31Slide Number 32Human Penis – transitional epithelium and �surrounding spongy cavernous of penal urethra