Lance C. Bridges, Ph.D. Curriculum Vitae

The Brody School of Medicine at ECU

Department of Biochemistry and Molecular Biology 115 Heart Drive, East Carolina Heart Institute Office #4413

Greenville, NC 27834 Office (252) 744-2882, Cell (501) 679-1749

email: bridgesl@ecu.edu

EDUCATION

Bachelor of Science, Biochemistry (honors degree), summa cum laude, John Brown University, Siloam Springs, AR, May 2000.

Doctor of Philosophy, Biochemistry, University of Oklahoma Health Sciences Center, Department of Biochemistry and Molecular Biology, Oklahoma City, OK, December 2003. Mentor: Ron D. Bowditch, Ph.D. Thesis: Leukocyte Integrin Recognition of ADAM Family Members: Delineation of Molecular Interactions of Homologous Mammalian Disintegrins.

POSITIONS

Assistant Professor, Department of Biochemistry and Molecular Biology, The Brody School of Medicine at East Carolina University, 2011-present

Assistant Professor, Department of Chemistry, University of Central Arkansas, 2006-2011 NIH Post-Doctoral Fellow, Department of Cell and Developmental Biology, University of

Virginia Health System, mentor: Judith M. White, Ph.D., 2004-2006 Graduate Research Assistant, Department of Biochemistry and Molecular Biology, The

University of Oklahoma Health Sciences Center, 2000-2003

FACULTY RESEARCH GRANTS AND AWARDS Extramural AWARDED: Research Corporation, Cottrell College Science Award ID: 7680, “Effect of Retinoids on Lymphocyte Metalloprotease Mediated Shedding” 2008-2011 NSF, MRI-R2, Project DBI-0959209, (Co-PI with Drs. Ben Rowley, Melissa Kelley, J.D. Swanson,

Steven Runge and Kari Naylor), “Acquisition of an Analytical Flow Cytometer for Natural Sciences and Teaching” 2009

Arkansas IDeA Networks of Biomedical Excellence (INBRE), equipment grant, (Co-PI with Drs. Melissa Kelley, J.D. Swanson, Kari Naylor, Brent Hill, Ben Rowley, and David Starkey), “Enhancing Cell Imaging Capabilities within the CNSM at the University of Central Arkansas” 2009 Arkansas IDeA Networks of Biomedical Excellence (INBRE), summer outreach: P20 RR16460, mentor: Dr. Jerry Ware, “A Novel Regulatory Mechanism of a Human Lymphocyte Metalloprotease ” 2007, 2008

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POST-DOCTORAL FELLOWSHIPS Individual NIH Kirschstein – National Research Service Award, 1F32GM073354-01,

“ADAM Proteolytic and Adhesive Activity in Cell Migration”, 2005-2006 Institutional NIH NRSA, National Cancer Institute, 5T32CA09109-28, Cancer Center

Training Fellowship, University of Virginia, 2004-2005

GRADUATE RESEARCH AWARDS

♦ University of Oklahoma Health Sciences Center, Outstanding Graduate, 2003 ♦ American Society for Cell Biology, Student Travel Award, 2003 ♦ Department of Biochemistry and Molecular Biology, University of Oklahoma Health Sciences

Center, Fred and Marie Gray Research Achievement Award, 2003 ♦ American Society of Hematology, Student Travel Award, 2001 ♦ University of Oklahoma Health Sciences Center, Graduate Program in Biomedical Sciences

(GPiBS), Provost’s Fellowship, 2000

TEACHING EXPERIENCE

East Carolina University Medical Biochemistry (BIOC7300), 9 lectures. My lectures in this first year medical course are to provide detailed information about amino acid and nitrogen metabolism in human health and disease. In addition, I concurrently mentor a small group conference of 10 first year medical students. This group meets to discuss 9 separate case studies throughout the duration of the course that relate current lecture topics to specific aspects of disease and treatment regiments. Biochemistry/Bioenergetics II: Regulation of Metabolism (BIOC/EXSS8320), 2012-current, 3 lectures. My lectures in this graduate course entail the central role of vitamin A and vitamin D metabolism in cell adhesion and migration. Specifically, metabolism of these specific vitamins in the context of embryonic development and immune surveillance is considered. Class time includes both didactic lecture and discussion of primary literature articles. I am currently the course coordinator for this graduate level course. Brody Summer Program for Future Doctors, 2012-current, 6 lectures. This non-credit course is used as a recruitment tool for talented undergraduates to given them a “taste” of medical school and get them on campus to view the facilities first hand. I coordinate six separate lectures all concerning amino acid and nitrogen metabolism. Foundations of Medicine, 1st year Medical Students. 2013-current. Along with a clinician, I serve as a preceptor for this active learning course. Small groups of approximately 10 students are allowed to identify and remedy their own learning issues with bona-fide medical cases. This course also includes a mentoring component in which various aspects of professionalism from study habits to self assessment are discussed. ECU School of Dental Medicine, 2012-current, 2 lectures. Lectures are given to the first-year dental class. An overview of nitrogen metabolism is delivered in two consecutive sessions with a “break-out” time for questions. Exam items are also developed that align with dental curriculum objectives.

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University of Central Arkansas Biochemistry Lab (CHEM4121), course coordinator, 2007-2011, 4 lab hrs/week. An upper division lab course that introduces biology/chemistry majors to seminal biochemistry techniques including but not limited to: agarose gel electrophoresis, TOPO-TA cloning, restriction mapping, ELISA, SDS-PAGE analysis, and Western Blotting. Emphasis is placed on hands-on experience, keeping an accurate, up-to-date notebook, and fostering independent thinking and technical trouble-shooting skills. Physiological Chemistry II (CHEM2450), course coordinator, 2006-2011, 3 lecture hrs/week with 2 lab hrs/week. This course is aimed to provide pre-nursing, dietetics, and occupational therapy majors with a topical overview of organic and biological chemistry. Course material is geared toward students with an innate interest in the health care professions and therefore strongly emphasizes the role and impact of chemical processes in a biological context (e.g. metabolic disorders). The objective of this course is to provide students with a working knowledge of chemistry by enabling them to understand how organic and biochemistry applies directly to health science fields in a useful and practical manner. Principles of Biochemistry (CHEM3300), course coordinator, 2006-2007, 3 lecture hrs/week. CHEM3300 is an introductory biochemistry course that focuses on the identity, structure, and function of biological macromolecules. A main goal is for students to employ their acquired biochemical knowledge in a logical manner for understanding disease diagnosis and treatment/intervention rationale. Primary literature is used to augment concepts from texts and acquaint students with how science is accurately communicated. Chemistry Seminar (CHEM 4112), course coordinator, 2008-2011, 1 lecture hr/week. Seminar is a capstone course in which students are afforded the opportunity to present basic scientific findings from the primary literature in oral format. Students are also required to attend departmental seminars to evaluate invited speakers in order to learn the art of scientific presentation. Medicinal Biochemistry (CHEM 4309), co-coordinator, team-taught, 2010; a capstone course that integrates synthetic organic chemistry, pharmacology, biochemistry, with complex bioethical considerations. The processes of drug design/synthesis, consideration of drug Absorption / Distribution / Metabolism / Elimination, specific drug mechanisms of action, and the structural / biochemical basis of the diseases they treat will be investigated for eight common classes of pharmaceuticals. Discussion of various complex ethical issues will also be interwoven throughout the semester. (My responsibilities included lectures entailing inflammatory processes and biomedical research ethics.) Piedemont Virginia Community College Biotechniques (BIO 170), 2004, 2 lecture hrs/week. This basic introductory course exposes individuals considering a science-based career to a real lab environment by providing hands-on experience with various lab techniques.

TEACHING AWARDS ECU Brody School of Medicine, 2014 Teaching Recognition Award. The Brody Medical Class of 2017 selected myself from among the entire basic science faculty for this award. I was recognized for demonstrating a steadfast commitment to medical education. It is awarded once per academic year to a single professor.

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EDUCATIONAL CONSULTING

American Institute for Research, External Reviewer, 2014-present American Institute for Research, Item Writer (biochemistry and metabolic emphasis), 2013-current National Math and Science Initiative (NMSI) consultant lecturer (GA, OK, NC, VA), 2012-present Arkansas Advanced Initiative for Math and Science (AAIMS) consultant lecturer, 2009-present

INVITED TALKS

ECU Brody School of Medicine, Pathology Ground Rounds, “The Role of Vitamin A Metabolites in

Immune Cell Adhesion” Greenville, NC, 2013

ECU Brody School of Medicine, Department of Pharmacology and Toxicology, “Retinoids Prompt Immune Cell Adhesion through RAR Nuclear Receptor Activity” Greenville, NC, 2013

ECU Brody School of Medicine, Department of Microbiology and Immunology, “Vitamin A oxidative

metabolites promote immune cell adhesion through two distinct mechanisms” Greenville, NC, 2012

UAMS Department of Biochemistry and Molecular Biology Seminar Series, “Novel Regulatory

Mechanisms of a Human Metalloprotease” Little Rock, AR, 2010 University of Oklahoma Health Sciences Center, Graduate Program in Biomedical Sciences (GPiBS)

10-year anniversary celebration, “Opportunities in Undergraduate Research and Education” Oklahoma City, OK 2010

Arkansas State University: American Chemical Society Student Affiliate Chapter, “ADAMs: Human Proteins Related to Snake Venom” Jonesboro, AR 2007 Keynote Address, Summer Undergraduate Research Experience (SURE), University of Oklahoma Health Sciences Center, “Attributes of a Successful Graduate Student; Is Graduate School for Me?” Oklahoma City, OK, 2004 John Brown University, “Where Does Basic Research Fit Into the Scientific Picture?” Siloam Springs, AR, 2004 John Brown University, “ADAMs (a disintegrin and metalloprotease), a Newly Identified

Protein Family with Diverse Biological Roles and Involvement in Various Pathological States” Siloam Springs, AR, 2001

SERVICE

Ad-hoc peer reviewer for: Journal of Cell Science, FEBS Letters, BBA- Molecular Cell Research,

Experimental Cell Research, Prostaglandins Lipids and Essential Fatty Acids Journal of Nutritional Biochemistry

ECU Brody School of Medicine, Administrative Search Committee, purpose: nationally recruit an

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Associate Dean for Undergraduate Medical Education, current

ECU Brody School of Medicine, Medical Student Promotion and Retention Committee, ad hoc member, 2014-current

ECU Brody School of Medicine 2nd Year Medical Curriculum Committee, 2013-current *Subcommittee: 3-year course review of active learning course, Foundations of Medicine ECU Brody School of Medicine, Medical Student Academic Achievement Program, 2011-current ECU Brody School of Medicine, Faculty Search Committee, purpose: recruit two new Assistant

Professors to the Department of Biochemistry and Molecular Biology, 2011-2012 ECU Brody School of Medicine, Graduate Student Thesis and Dissertation Committee Member 9 Ph.D. candidates (active), 3 Ph.D. (conferred), 1 M.S. (active), 1 M.S. (conferred) UCA, Departmental Seminar Coordinator, invite and schedule scientific speakers, 2008-2011 Student Undergraduate Research Fellowship (SURF) of Arkansas, biochemistry reviewer, 2009-2010 UCA, Department of Chemistry, Chemistry lab coordinator search committee, purpose: advertise and successfully recruit a laboratory coordinator for the Department of Chemistry, 2009 UCA, Department of Chemistry, Curriculum Committee, purpose: develop and implement an American Chemical Society (ACS) certified Biochemistry degree track, 2007 UCA, Department of Chemistry, Curriculum Committee, purpose: establish program requirements and selection criteria, Masters of Science in Education with chemistry emphasis, 2008 Category Judge, Arkansas Junior Academy of Sciences, 2007-2009

PEER REVIEWED PUBLICATIONS

1. Charles M. Roberts, Patricia H. Tani, Lance C. Bridges, Zoltan Laszik, and Ron D. Bowditch. (1999) MDC-L, a Novel Metalloprotease Disintegrin Cysteine-Rich Protein Family Member Expressed by Human Lymphocytes. J. Biol. Chem. 274:29251-29259 2. Lance C. Bridges, Patricia H. Tani, Krista R. Hanson, Charles M. Roberts, Matthew B. Judkins, and Ron D. Bowditch. (2002). The Lymphocyte Metalloprotease MDC-L (ADAM28) is a Ligand for the Integrin α4β1. J. Biol. Chem. 277:3784-3792 3. Lance C. Bridges, Krista R. Hanson, Patricia H. Tani, Timothy Mather, and Ron D. Bowditch. (2003) Integrin α4β1 Dependent Adhesion to ADAM28 (MDC-L) Requires an Extended Surface of the Disintegrin Domain. Biochemistry 42:3734-3741 4. Lance C. Bridges, Dean Sheppard, and Ron D. Bowditch. (2005) ADAM Disintegrin-like Domain

Recognition by the Lymphocyte Integrins α4β1 and α4β7. Biochem. J. 387(1):101-108

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5. Lance C. Bridges and Ron D. Bowditch. (2005) ADAM-Integrin Interactions; Potential Integrin Regulated Ectodomain Shedding Activity. Curr. Pharm. Design 11(7):837-847

6. Jing Huang, Lance C. Bridges, and Judith M. White. (2005) Selective Modulation of Integrin Mediated Cell Migration by Distinct ADAM Family Members. Molec. Biol. Cell 16(10):4982-4991 7. Lance C. Bridges, Joshuah D. Lingo, Rachel A. Grandon, and Melissa D. Kelley. (2008) All- trans-Retinoic Acid Induces Integrin-independent B-cell Adhesion to ADAM Disintegrin Domains. Biochemistry 47(15):4544-4551 8. Shuo Wei, Charles A. Whittaker, Guofeng Xu, Lance C. Bridges, Anoop Shah, Judith M. White and Douglas W. DeSimone. (2010) Conservation and Divergence of ADAM Family Proteins in the Xenopus Genome. BMC Evolutionary Biology, 10:211 9. Shuo Wei, Guofeng Xu, Lance C. Bridges, Phoebe Williams, Judith M. White, and Douglas W. DeSimone. (2010) ADAM 13 Induces Cranial Neural Crest by Cleaving Class B Ephrins and Regulating Wnt Signaling. Developmental Cell, 19(2):345-352 10. Shuo Wei, Guofeng Xu, Lance C. Bridges, Phoebe Williams, T. Nakayama, Robert M. Grainger Judith M. White, and Douglas W. DeSimone. (2012) Roles of ADAM13-regulated Wnt activity in early Xenopus eye development. Developmental Biology, 363(1):147-154.

11. Jarrett T. Whelan, Jianming Chen, Jabin Miller, Rebekah L. Morrow, Joshuah D. Lingo, Kaitlin Merrell, Saame Raza Shaikh, and Lance C. Bridges. (2013) 9-cis-Retinoic Acid Promotes Cell Adhesion Through Integrin Dependent and Independent Mechanisms Across Immune Lineages. Journal of Nutritional Biochemistry, 24(5):832-841

12. Benjamin Drew Rockett, Mark Melton, Mitchel Harris, Lance C. Bridges, and Saame Raza Shaikh. (2013) Fish Oil Disrupts MHC Class II Lateral Organization on the B-cell Side of the Immunological Synapse Independent of B-T Cell Adhesion. Journal of Nutritional Biochemistry, 24(11):1810-1816 13. Jarrett T. Whelan, Lei Wang, Jianming Chen, Meagan E. Metts, Taj A. Nasser, Liam J. McGoldrick, and Lance C. Bridges. (2014) Retinoids Stimulate Integrin-Independent Immune Cell Adhesion Through RAR-Alpha Activity. Biochem Biophys Res Commun. 454(4):537-542

BOOK CHAPTERS

1. Judith M. White, Lance C. Bridges, Douglas W. DeSimone, Monika Tomczuk, Tyra G. Wolfsberg. (2005) Introduction to the ADAM family. Proteases in Biology and Diseases, N.M. Hooper and U. Lendeckel, Editors. Springer. vol 4: pgs 1-28