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Lecture 7:

Gene Cloning and Analysis

Part II

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Cloning essentials

1. DNA that contains the desired gene(insert)

2. Plasmid (vector)

3. Competent cells (host)

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Can a single E.coli take up two plasmids?

Plasmid incompatibility is deined as the ail!reo t"o plasmids co#resident in the same cell tobe stably inherited

Plasmids "ith similar origins o replication$ andthereore similar systems or reg!lating thereplication and segregation o the plasmid$ aresaid to be in the same %incompatibility gro!p%and cannot both be passed do"n consistently toda!ghter cells

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Discussion

&ene Cloning Proced!res