LIST OF CONTENTS

ForewordPrefaceList of ContentsList of AuthorsList of Participants

VOLUME 1

Non-GM Biotechnological Approaches

Micropropagation of Malus sieboldii Hybrids Resistant to AppleProliferation DiseaseA.M. Ciccotti, C. Bisognin, I. Battocletti, A. Salvadori, M. Herdemertens,M. Wallbraun and W. Jarausch

Micropropagation, Rooting and Acclimatization of Blackberry'Agavam'L. Lepse and V. Laugale

Genotypic Differences in Shoot Multiplication amoÍgFive CitrusRootstocks In VitroS. Sen and V. Dhawan

Citrus limon Micropropagation: Effect of Different Phytohormones onMultiplication and RootingO. Pérez-Tornero, C. Tallón and I. Porras

Micropropagation of 'Troyer' Citrange lPoncirus trifoliata (L.) Rat. x C.sinensis (L.) OsbecklS. Sen and V. Dhawan

Propagation of Some Date Palm Cultivars by Using Tissue Culture MethodsI. Pinker, Y.M. Ibraheem and M.H. Bóhme

The Influence of Imidazole Fungicides on Multiplication In Vitro of LowVigorous Pear and Cherry RootstocksDj. Ruáió, T. Vujovié, R. Cerovió and M. Kuzmanovió

In Vitro Proliferation of Newly Bred Czech Pear CultivarsJ. Sedlak and F. Paprsfein

Photoautotrophic Micropropagation for Cost-Effective and SuccessfulClonal Multiplication of Woody Fruit CropsK.J. Pawan, V.A. Kumar, S. Shah and A. Kumar

In Vitro Propagation of Wild European Plum (Prunus xdomestica L.), aRare and Endangered SpeciesH.Y. Yuan, Y.X. Wu, K. Liao, W.J. Geng, J. L¡, Z. Xu and T. Wang

Development of a Micropropagation Protocol for the Romanian Quince(Cydonia oblonga) Cultivar 'Aurii' and Rootstocks 'BN70' and 'A Type'A. Giorgota, S. Preda, M. Isac and M. Tulvinschi

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Micropropagation of Selected Trees of Arbutus unedo L. throueh AxillaruShoot Proli feration and Somatic EmbrvosenesisF. Gomes, M.L. Lope.s, T. Santos and i.M. Canhoto

lmproved.Micropropagation of Walnut (Juglans regiaL) on MediaOptimized for Growth Based upon Mineraf Conteni of Walnut SeedK. Vahdati, E. NajaJian Ashrafi, H. Ebrahimzadeh ancl M. Mirntasc¡umi

In Vitro Propagation of Some Grape RootstocksS.Z. El-Agamy, T.K. El-Mahdy and A.A. Mohamed

In Vitro Propagation of Zizyphus spina-christiL.S.Z. El-Agamy, T.K. El-Mahdy and D.Sh. El-Kasas

Regeneration and Field Evaluation of Banana (.Musa acurninata, AAAGroup) Plants from Proliferating Infl orescence-Derived EmbryogenicSuspension CulturesJ.A.- Cabrera-García, J. Cabrera-Cabrera, J.B. pérez-Hernánclez andM.T. Cruz-Bacallado

Factors Affecting Somatic Embryogenesis Induction and Development inFe ij oo sel low i a na Berg.J.M. Canhoto, S.l. Correia and C.l. Morques

Somatic Embryogenesis in Tamarillo (Cyphomandra betacea): RecentAdvancesS.I. Correia, M.L. Lopes and J.M. Cenhoto

Effect of carbohydrate Source and Polyethylene Glycol on Maturation andGe¡mination of Somatic Embryos in Walnut (Jugtans regia L.)H. Bahrami Sirmandi and K. Vahdati

In Vitro Germination of Stoneless Seeds and Isolated Embrvos of 14Sicilian Cultivars of Olea europaeaL.M.A. Gcrmana, G. Patricolo and B. Chiancone

In Vitro Germination and Seedling Development of Caper (Capparisspinosa L.) Mature SeedsM.A. Gernana and B. Chiancc¡ne

Introducing New Genes into 'Thompson Seedless' GrapesS.Z. El-Agamy, H.A.A. Galil and M.A.M. Elsysy

Efficient Shoot Regeneration System of Pear Rootstock OHF 333 (pvruscommunis L.) LeavesL.R. Nacheva, P.S. Gercheva and V.T. Dzhuyinov

Impact of Exogenous Sucrose, Raff nose and Proline on Cold Acclimationof Strawberry In VitroV. Lukoievióiuté, R. Rugienius, A. Sasnauskas, V. Stanys and ó. Bobinas

Commercialiy Feasible Protocol for Rooting and Acciimatization ofM icropropagated Apple RoorsrocksM. Modgil, T. Sharma and M. Thakur

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In Vitro Rooting Improvement of Adult Pistachio, Pistacia vera L. ' Atll'E. Tilkat, A. Onay and Y. Ozden Tokatli

Rooting and Multiplication Ability of Persian Walnut as Influenced byMotherstock Vigor and PrecocityK. Vahdati, M. Mirmasoumi and R. Rezaee

Cryopreservation of Papaya (Carica papaya L.) Seed: OvercomingDormancy and Optimizing Seed Desiccation and Storage ConditionsS.E. Ashmore, R.A. Drew, C. O'Brien and A. Parist

Advances in the Cryopreservation of Fruit Plant Germplasm at the CNR-IVALSA Institute of FlorenceM. Lambardi, C. Benelli, A. De Carlo and A. Previati

Application of Cryopreservation for Pistachio Germplasm,Conserr¿ationY.- ()zden Tokatli, E.A. Ozudogru, H. Akdemir, F. Gumusel and A. De Carlo

Cryopreservation of Strawberry cv. 'Senga Sengana'by DropletVitrificationI. Pinker, A. Halmagi, K. Olbricht and F. Ehrig

Preliminary Results of In Vitro Thermotherapy of Pear CultivarsF. Paprstein, J. Sedlak, L. Talacko, L. Svobodova, P. Zeman, J. Polak andM. Hassan

Thermotherapy of Apricot CultivarsB. Kíiían and E. Ondruíiková

The Functional Properties of Sweet Cherry as a New Criterion in a BreedingProsramH.lú. Díaz-Mula, D. Valero, P.J. Zapata, F. Guillén, S. Castillo,D. Martínez-Romero and M. Serrano

Evaluation of Dry Matter in Sour Cherry (Prunus cerasus L.)C. Grafe, M. Hófer and M. Schuster

Development of a Suitable Protocol to Overcome Hyperhydricity in Apple(Malus sp.) during In Vitro RegenerationM.K. Hcihnle and G. Weber

Mineral Composition of Some Walnut Cultivars (Juglans regiaL.) forEvaluation of Ionome and Ionomics under Salt Stress ConditionN. Lotfi, K. Vahdati, B. Kholdebarin, D. Hassani and R. Amiri

A Review on Bioactive Compounds in Black Currants (Ribes nigrumL.)and Their Potential Health-Promoting PropertiesR. Karialainen, M. Anttonen, N. Saviranta, D. Stewart, G.J. McDougall,H. Hií2, P. Mattila and R. Tr)rrónen

Evaluation of Means to Increase the Content of Bioactive PhenolicComoounds in Soft FruitsM.J. Anttonen and R.O. Karialainen

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Effect of Hexanal Treatment on Postharvest Quality of 'Darselect'

Strawberry (Fragaría xananassa Duch.) FruitH.Y. Yuan, X.L. Hu, X. Li, Y.H. Li and P. Gopinadhan

Study on Softening of Vinagrillo (Averrhoa bilimbi) Fruits during RipeningA. Guadarrama and E. González

Sterilization of Non-Autoclavable Vessels and Culture Media by SodiumHvoochlorite for In Vitro CultureK.

-Matsumoto, M.C.F. Coelho, D.C. Monte and J.B. Teixeira

The Reaction of Raspberry and Blackberry Cultivars to Drought StressSimulated In Vitro by Polyethylene Glycol (PEG) 6000T. Orlikowska, D. Kucharska and M. Horbowicz

Effect of Sucrose Level on the Photosynthetic Ability of In Vitro CultivatedApple Rootstock MM 106L. Nacheva, K. Ivanova and Z. Zlatev

New Strategies in Gene Technology

Effect of Antibiotics on Regeneration and Elimination of Bacteria duringGene Transfer in AppleM. Modgil and R. Sharma

Preliminary Molecular Evidence that Quantitative Polymerase ChainReaction Detects Chimeras in Transgenic PlantsM. Faize, L. Faize and L. Burgos

A Preliminary Study on Genotype-Ind ep endent A gr o b a c t er i um -MediafedTransformation Method to Obtain Genetically Engineered Apricot PlantsH. Wang, M.D. Nortes, M. Faize, L. Faize, S. López-Noguera,C. García-Almodovar, I.M.G. Padilla, N. Alburquerque, C. Petri,L. Burgos and O. Pérez-Tornero

A High-Throughput Transfotmation System in Plum (Prunus domesticaL.)Useful for Functional Genomics in RosaceaeC. Petri, K. Webb, Ch. Dardick and R. Scorza

Agrobacterium-Mediated Transformation of a Walnut CultivarH. Wang, M.D. Nortes, L. Burgos, N. Alburquerque and J.M. López

Agrobacterium-Mediated Transformation of Olive (Olea europaea L.)Embryogenic CulturesR. Torreblanca, E. Palomo-Rios, ,S. Cerezo, J.A. Mercado andF. Pliego-Alfaro

Systemic Acquired Siiencing of a gusA Transgene in AppleC. Hcittasch, H. Flachowslq,, M.-V. Hanke, I. Szankowski, P. Wolff andS. Waidmann

Silencing in Genetically Engineered Prunus domestica Provides Durableand Safe Resistance to Plum pox vlrzs (Sharka Disease)M. Ravelonandro and R. Scorza

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VOLU]

GenomBiotech

Genes IMoleculY. Han t

Using FResistanJ.L. NorJ.M. CetR.E. Fat

The GenA.C. AlltK. Lin-14

IdentificrGrapevirL. Blank,

QTL andProgenyF. Dunet

The PolyPercepticS. Marió,

IdentificaJ.M. SoriM. van K

ResistancG.A.L, BIA. Patocc

First EvicApple SciR. Paris,,V. Gualdi

Role of S,Y. Zhu, Y.

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Developmerrl ol 'Cisgenic AppJcs ."r ' i th Durablc [{csistance to A¡tp1c ScabS.G. "/oshi,.J.X,l . Soriuno, A. Korl, \ lcc,. l .( i . St 'hut¡rr, l r .1. l{ t 'ctts, l : . ,Jtt tr¡hst ' t tttnd H.J. Schr¡ttlc¡t

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Cienornics - {Jnr{er¡r inning Technolog,l , fbl ' N{olceular Brccding amdtsiotechnoiogy'

( lcncs [,ncoc]ing Flavonoicl 3'-Hyclroxylase in AppJc and Thcir laggcdMolecLrlar M¿rrhers1'. IIttn und S.S. IQ¡rhun

Using l :Lurct ional Ccnolr ics to lclenti fy MolecLl lar N4¿rrl<crs t irr I ' i rc L3l ightl{esislance (Et 'v ' iniu unt¡, |ott tru) in Apple (Mu|tn)./ .L. Nr¡rcl l i , D.A. Lal l i , C.L. Bas.yett, M.E. Ll/ i . tntey'ski, S.l : . Gttrt l inct ' ,J.ll. Celtott. D.R. Bowatte, C M. ('urli.sle, ll. Mulnr¡.t', Il.S. 1l¿l¡rintkla.R.1,. l:'an'e ll ./r., A.M. Boldo, M.Il. llrtrncr ttnd V.(1.il|. Btts

' fhc Gcnonr ics o f FrLr i t Qua l i t_v

A.(1. Al l t tn, W.A. Luing, R.f . i : :¡1¿.¡. \ ,1. . \ l t ,ntt l i t t t ¡ , () . l ) t 'untantt. I l l / t ,nt ' .r ,K. l.in-Wung, A.P. Dare und R.P. Hcllens'

lclcnti l icat ion ol-Clancl iclatc Cicncs Involvccl in [rhl, l loxera ftesisLancc inGrapcl inc Rootstocl<sL. Illttnl¡, A. Dielrich, K. Lincrt, T. (iaicr, T. Iliol/ant{ i\1.-l}. "\tltt'ótlcr

Q'l l - and Candidatc Cr:nc Mapping fbl Arona [,orl l tounds in ihc ApplcProgenl 'L) iscorrery' x "Priua'

F. Dunt,nonn,,l. Bt¡uclichct.'.tkaiu, C. Grulc, l). Llrith unJ I1'.L. Lí,t'hu'

The Po lymorph ism o l ' the Genes Invo lved in E thv lcnc l l i osvn thcs is andPcrccptiou in ApplcS |lut'i(, ü1. Luki(: and R.l. Br¡,ikovi(:

Iclcnti f icat ion ancl Mapping o1'a Ncrr,Apple Scab I{esistauce ( lenc.J.tr|. Sori¿uto, S. ./o.thi, ll. Groeny'old, Y. Nortrtlijli, B. HL:nkctt,Il. t.,on Ku¿tttu'an und fl..J. SchouÍcn

I{csrstancc (cnc Analogrrcs of lk 'rV/2 in AppleG.tl.l,. Ilt'oggini, P. (lolli, (]. Purroy,icini, (). Ges.slct', L. ('tunli'¿ttt't'.sthi utttlA. Palot 'chi

l ' ' i rst F.viclcncc o1'cl- l l l ) l -C Fif Í icicncl ' f i rr an ALrtorl¿rted cDNA-AF l- l) in thcApple Scab [{esistance MoclelR. Put'i.¡, L. Dondini, S. Torfat'ini, S. Scut,s't¡t,ini, l). Ilastiu, l'. l,fttnÍt¡t,tuti,[. Guolcli ond P. Pi//hnclli

I lole of SlI¡15 Ccnc in l :c Uptake Mechanisrr c¡ l ' l , l t t l t t .s siuof inenti .sY. Zhtt, Y. Wang,,/. Kong,./. l4/ong, X. Zhttng ond ll. llrrn

Parent Identi f icat ion o1'HLrn.elarian AppJe {- iul t ivars Using SSIt N,larl ters1. I{it'r?lt, X,1.7-óth, tl. ['ctlrj:c..J. llulú.tz and T. Dcált

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Identification of Apple lree Cultivars Growing in Belarus Using SSR-

Y.^í1ffiu',"" ich and z' A Kaztovskav a

Preliminary Molecular Marker Maps Con{r¡c^f-A..Uv Using an tnterspecif,ic

¿i;t"X:Xf :!X' u, X:iü,;rf:d P us s u, en s'¡s

Functional 2iversitv for Response to-lvatelDefl:f ';1fr:?$'3lTillJrr.o uvS;;;;ü;v cenotypes Compiared to Their Genettc

f:k:-XTtri van Labeke and r de Riek

Isolation and Characterization of LTR Retrotransposons from Strawberry

Genome 'I' Dai, x Gao' H' Li and z zhangY. Ma, H Sun, G' Lhao' r

Microsatellite Mar\eJs (ss^R) as lTool T *i.*L" Identification of Sweet

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t' i, ii,, t av i u m I and .S.our Chen-v ( P r u n u s c e ras u s ) -'il 'iii, n. Wang' M' sainúe''o' MÓller and W Hartntann

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Comparative Genomtcs of the Evergrowing Locus among Apricot' Peach 523

*,r;:)2: z. L¡. c.L. Reighard, D.c. Bietcnberg. A.L. Lawon-Rauh and

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Targeted lsolation of nonTlR-NBS-LRR Resistance Gene Analogs tn 531

AortcotA. Gutermuth and A' PedrYc

Ootimization of the Preparation of ds cDNA from Flower Buds of Apncot

io"lü. .oN¡'-AFLP AnalYst*. r,Oro

.1. Cechova, M. Baranek c

Almond Genetic Linkage Map and Quantitative Trait Loci for Flowering

HHilá | s ó,i"' ¿if]zi :v' i,:;i"F" f'1"'' o n a n'l) M S e d g t e v

Assessment of Genetic Variation in Citrus Germpiasm of South of lran

"^:'Pfr,:;,kV::;:í:tbhi, K' vahdati' P' Gotein' A Nabipour and M Danesh

Transcriptomic Analysrs of Ethylene-lnduced Tolerance to Non-Chilling

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Transferability of Malus x domestica Microsatellite Markers to otherSoecies and Genera of the Maloideae SubfamilyL'. Vanwynsberghe, L. Decq and J. Keulemans

AFLP-Derived Methods as a Tool for Study of Genomic, Epigenomic andTranscriptomic Changes in Stressed Grapevine-PlantsM. Baranek, B. Kíiáan, J. Raddová, E Ondruííková and M' Pidra

Clonal Selection within Grapevine 'Askari' by Molecular MarkersA. Ebadi, R. Nikkhah, R. Naghavi, M. Cresti, M. Scali, R. Vignani andJ. Bigliazzi

The Anthocyanin and Flavonol Profile in vitis vinfelq Intraspecific-HybridsA. Hernáncl'ez Jiménez, J.M. López-Roca' E. Gómez Plaza' R' Gil-Muñozand A. Martinez Cutillas

Molecular Characterization and Analysis of Geographical Differentiation ofIndian Mango (Mangifera indicaL.) GermplasmS. Singh and K.V. Bhat

Assessing the Use of Heterologous Oligonucleotide Micro¿rrfYs for ^Transcrip"tomics in a Non-Model Speciés; Application to the Study ofDroueht Stress in M¿¡saM.W.-Davey, J. Keulemans, N. Graham, S'7. May, B. Vanholmeand R. Swennen

Genetic Diversity Sndy in Annona squatnosa by Morphological'Biochemical and RAPD MarkersS.G. Bharad, P.L. Kulwal and S.A. Bagal

Construction of a Reliable PCR Marker for Selecting Pollination Constant -and No-n-Astringent (PCNA) Tlpe Offspring among Breeding Population ot'Persimmon (D i o s py r o s kaki Thunb.)K. Yonemori, T. Akagi and S. Kanzaki

Advances in Agronomic Important Traits

RNAi-Silencing of MdTFL I Induces Early Flowering-in AppleI. Szankowski,

-5. Woidmonn, A. El-Din Saad Omar, H. Flachowslq',

C. Hdttasch and M.-V. Hanke

Characferizatton of Transgenic Apples Expressing Ihe HrpN GeneE. Vergne, S. Sourice, F. Dupuis' k. Loridon. T. Dugé de Bernonville,M.N. Erisset and E. Chevreau

Ectopic Expression of a Spinach SOD Gene in Young Apple TreesEnhances Abiotic Stress ResistanceT.S. Artlip, M.E. Wisniewski, D. Macarisin and J.L' Norelli

Towards the Production of Stress Tolerant Grapevine CultivarsA. Zok, R. Oláh, B.P. Kós, É ttid"g, V.G. Honáth, Gy. Váradi, E' Szegediand B. Báló

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9J,"^:lt^::9,gharacterization of VvChiF III Gene from Vitis vini/éra cv.i talne seedlessA. Ebadi, S.A. Salami, M.R. Naghavi ancl I.B. Dry

Tle Development of Ge'etic Resistance fo prutn pox virus inTransgenicNicof iana benthamiana and. prunus dontesticoA Wang, L.,Tian, T.-5. Huang, D.C.W. Brr¡wn, A.M. Syircev, L.W. Stobbs,B. Miki and H. San/agon

Stilbene Synthase Gene Transfer Resulted in Down Regulation ofFndogenous chalcone Synth_ase in Strawberry (Fragarn x ananassar andLed to the Identi l'rcation -of

No vel phenyipropanoi¿'C¡u.oriá.K. Hanhineva, M.J. AntÍonen, H, Kokká,'p. Soin¡nen, R. Laatikainen,S. Kcirenlampí, L Rogachev and A. Ahaioni

Transcriptomic and proteomic Response of Fruit Trees to Abiotic StressM. wisniewski, C- Bassert, D. Mac-arísin, J. Norei¡, i. Áritil""i i"iá"*on

Plant !.ize,Control in Apple through Genetic EngineeringL.H. Zhu, X.y. Li, L. KAI¿ and M.-Welancler

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T9 lreJiminary Characterisation of rhe promoter Region of the I _Amtnocyctoprop^ane- I -Carboxylate Synthase GeneJ. vt. uran(Eto. 5. l¿rtat'ini. p. Neg.i and S. Sansuvini

The switch to Flowering: Genes Involved in Floral rnduction of the AppleCultivar'Pinova'and th-e Role of the ffo*errng Ge;:;A,I; if vr !rr! / rPvr!

C. Hcittasch, H. Flachowslry, M.-V. Hanke, S. f"i*orn,A. Gau and D. Kapturska

Iry j l S.1 Regulat ing Cenes are Di f ferenr ly Expressed a l ier Cross_roi l rnarron. Sei l -poi l rnal ron and r reatment wi th p lant crou th Regulators inMalus x dr.¡mesfica cv. 'Jonaeold,

I 2r^^::!:J. Keulemans. E. prinsen, J. Vcrcamnten, L. Denruyfer antl

A. lJ()mqnd

Biosal'ety and Risk Assessment of GM Crops

Environmental Risk Assessment of GM plants at the European LevelJ. Schiemann

First Results on the Efrect of lncreased chitinase Expression in TransgenicApple Trees on Mycorrhization with Gromus intrararJices and G. mt¡síeaer S.c!1y/yr, F. Busiot, H. Ftachowstqt, M. f. H;"i;, A. roitiiiand S. Kónig

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