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Live food aquaculture training coursewww.aquatrain.org
Rotifers
their role and production requirements
Live food aquaculture training coursewww.aquatrain.org
Reproduction
Size
Tolerance
Mobility
Bio-encapsulation
Mass production
Live food aquaculture training coursewww.aquatrain.org
Life cycle B. plicatilis
Live food aquaculture training coursewww.aquatrain.org
Tank: Any Size/Shape (2-6 m3) Temp.: 25-27 °C (Preheated Water/Heat Exchange) Salinity: 18-25 ppt Oxygen: 5-7 ppm (Pure Oxygen) pH: 7.5-8.5 NH3/NH4+: < 10 mg/l NH3: < 1 mg/l Water: Filtered (1 m), UV, Chlorinated/Neutralized Aeration - Reasonably Strong - Airstones Illumination: Low Light Conditions Floc traps Batch Culture: 3-5 days
Rotifer production – optimal culture and enrichment conditions
Live food aquaculture training coursewww.aquatrain.org
Rotifer production in 3m3 tanks
Live food aquaculture training coursewww.aquatrain.org
Low density rotifer culture
Sea bream
General assumptions
1) Av. daily rotifer consumption/m3 larval volume (M) 10
2) Rotifer production volume/100M daily production (m3) 1,5
3) Period of rotifer feeding (days) 20
4) Culture Selco needed per 100M rotifers produced (kg) 0,2
5) Culture Selco price (drs/kg) 11234
Annual production target (2g juveniles)
No of larval batches 5 5 5 5
Initial larval rearing vol (m3) 62 123 185 308
Daily rotifer requirement (M) 615 1.231 1.846 3.077
Rotifer production volume (m3) 9 18 28 46
No of 2.8m3 tanks needed 3 7 10 16
No of 4m3 tanks required 0 0 0 0
Total rotifer production volume (m3) 8 20 28 45
Total Culture Selco requirement (kg) 123 246 369 615
Requirements 2.000.000 4.000.000 6.000.000 10.000.000
Cost of Culture Selco (Mdrs/yr) 1,38 2,77 4,15 6,91
Live food aquaculture training coursewww.aquatrain.org
Rotifer culture using algae excellent results with excellent results with
algae but expensivealgae but expensive
time consuming (culture time consuming (culture of algae + counting)of algae + counting)
availability of algaeavailability of algae
quality of the rotifers quality of the rotifers dependent on quality of dependent on quality of the algaethe algae
Live food aquaculture training coursewww.aquatrain.org
Rotifer culture using algae and yeast
variable results in variable results in rotifer survival rotifer survival depending on the depending on the quality of algae and quality of algae and freshness of the yeastfreshness of the yeast
unexpected mortalities unexpected mortalities (total crashes) due to (total crashes) due to lack of standardisationlack of standardisation
variability in rotifer variability in rotifer quality (nutrition, quality (nutrition, microbiology)microbiology)
Live food aquaculture training coursewww.aquatrain.org
Batch culture of rotifers on algae and yeast
harvest
drain culture water
Culture period6-10 days
feeding
Re-inoculation
Live food aquaculture training coursewww.aquatrain.org
Medium density rotifer culture
Sea bream
General assumptions
1) Av. daily rotifer consumption/m3 larval volume (M) 10
2) Rotifer production volume/100M daily production (m3) 0,4
3) Period of rotifer feeding (days) 20
4) Culture Selco 3000 needed per 100M rotifers produced (kg) 0,20
5) Culture Selco price (drs/kg)
Annual production target (2g juveniles)
No of larval batches 5 5 5 5
Initial larval rearing vol (m3) 62 123 185 308
Daily rotifer requirement (M) 615 1.231 1.846 3.077
Rotifer production volume (m3) 2 5 7 12
No of 2.8m3 tanks needed 3 7 10 16
No of 4m3 tanks required 0 0 0 0
Total rotifer production volume (m3) 8 20 28 45
Total Culture Selco 3000 requirement (kg) 123 246 369 615
Requirements 2.000.000 4.000.000 6.000.000 10.000.000
Cost of Culture Selco (Mdrs/yr) 0,00 0,00 0,00 0,00
Live food aquaculture training coursewww.aquatrain.org
Rotifer growth comparison CS v’s CS3000CS 3000-MD vs. CS-LD
0
200
400
600
800
1000
1200
1400
1600
1800
0 1 2 3
Time (days)
Ro
tife
r d
en
sit
y (
#R
/ml)
CS 3000-MD
CS-LD
Live food aquaculture training coursewww.aquatrain.org
Specific Growth Rate in MD batch culture
SGR in MD batch culture: CS vs. CS 3000
0
0.10.2
0.30.4
0.5
CS 3000 CS
SG
R (d
^-1)
Live food aquaculture training coursewww.aquatrain.org
Method of determining rotifer growth and condition– mean specific growth rates
SGR = ln (Final Number)-ln (Initial number) No. of days of culture
ln = natural log
Useful for:- Following feeding experimentsQuantitatively assessing culture stability
Live food aquaculture training coursewww.aquatrain.org
Rotifer production methodologiesCulture Selco 3000 and O2
Live food aquaculture training coursewww.aquatrain.org
Rotifer Production
MAM2
M: Masters
A: Culture (2-6 m3)
B: Inoculum (500 R/ml)
C: Enrichment
(500-1000 R/ml)
Larval Tanks
B C
30% 70%
Live food aquaculture training coursewww.aquatrain.org
Culture Selco 3000 strategy
Live food aquaculture training coursewww.aquatrain.org
Traditional rotifer batch culture
Live food aquaculture training coursewww.aquatrain.org
High density rotifer production system
Live food aquaculture training coursewww.aquatrain.org
Rotifer harvesting
Live food aquaculture training coursewww.aquatrain.org
Harvesting of rotifers
Live food aquaculture training coursewww.aquatrain.org
Transfer of rotifers
Live food aquaculture training coursewww.aquatrain.org
Recirculated ultra-high density rotifer culture ~5000 rotifers + /ml
Live food aquaculture training coursewww.aquatrain.org
Commercially available rotifer and artemia cultivation and enrichment products
Live food aquaculture training coursewww.aquatrain.org
Fatty acid enrichment levels in Rotifers
mg/g mg/gEnriched rotifers EPA DHANannochloropsis 7,3 2,2Nannochloropsis sp. 3,44 0,6Chlorella 3,75 0,6Isochrysis sp. 1,87 1,25Protein Selco 8,125 8,125Super Selco 33,12 36,25DHA Super Selco 41,4 68
