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LSBRLSBR
Single particle processing workflow in
BsoftBernard Heymann
SPA Processing Steps
Picking
Corrections/Modifications (CTF, mask)
Alignment
Reconstruction
Quality assessment (Resolution)
Bsoft SPA Workflow
Parameter Organization
Parameter Handling
Flat files:
Text files with tagged parameters
Images referenced through tags
Multiple formats: STAR & XML
Backwards compatible
Types of Bsoft parameter files:1.Micrograph/reconstruction parameter files2.Modeling parameter files
Quality Assessment
Reconstruction:Resolution/spatial frequency limit.
Modification curve (sharpening, B-factor)
Number of particles in reconstruction
Defocus range, astigmatism range
View distribution
Coverage/Completeness (up to spatial frequency limit)
Particle FOM distribution
Resolution: FSC curve or similar
ModelingFitting statistics
Reconstruction assessment
View distribution ResolutionFOM distribution