MB&B 208: Molecular Biology

Fall 2006

MWF, 10-11

Hall-Atwater 84

http://sholmes.web.wesleyan.edu/wescourses/2006f/mbb208/01/

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The “Central Dogma” of Molecular Biology

Gene definition:

Classical genetics: The functional unit of inheritance.

Molecular genetics: The nucleic acid sequence that is necessary for the synthesis of a functional polypeptide (protein) or RNA.

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Transcription initiation in eukaryotes

RNA polymerase: CTD = carboxy terminal domain

GTF’s = general transcription factors

TFIID: includes TBP, binds TATA box

TFIIH: helicase and CTD kinase

Initiation: phosphorylation of CTD by TFIIH

GTF’s do not remain associated with transcribing polymerase

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TBP (TATA binding protein)

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Gene transcription: role of transcription factors1. Recruitment of RNA polymerase2. Overcome inhibition by chromatin structure

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