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Wednesday, February 9, 2011
Objective: Students will be able to sequence the steps involved in protein synthesis.
Bellringer:
What is the difference between protein synthesis and DNA replication?
Wednesday, February 9, 2011
Objective: Students will be able to sequence the steps involved in protein synthesis.
Bellringer:
What is the difference between protein synthesis and DNA replication?
Wednesday, February 9, 2011
Objective: Students will be able to sequence the steps involved in protein synthesis.
Bellringer:
What is the difference between protein synthesis and DNA replication?
Wednesday, February 9, 2011last chance to view BR - 1 min
Objective: Students will be able to sequence the steps involved in protein synthesis.
Bellringer:
What is the difference between protein synthesis and DNA replication?
Agenda
1. Bellringer
2. Reminder: Assignments Due List
3. Finish Notes
4. Work Time: Problem Set & Old Assignments
Tomorrow: DNA Lab
So far this semester, you should have completed…
I will be entering in these grades, so get these in please!
Reminder: Assignments List
Day Assigned Date AssignmentMonday 1/31 BrainPop DNATuesday 2/1 DNA OrigamiMonday 2/7 MiniLab 11.1 (p. 293)Tuesday 2/8 Quiz – Chapter 11Tuesday 2/8 DNA & RNA Webquest
Wednesday 2/9 DNA & RNA Problem Set
DNA VOCAB. – Test yourself!
1. DNA2. Nitrogenous
base3. Double helix4. DNA replication5. Nucleotide6. Purines7. Pyrimidines8. Guanine (G)9. Adenine (A)10.Cytosine (C)11.Thymine (T)
A. Carbon ring structure contains 1+ Nitrogen, includes: G, A, C, T
B. Subunit of nucleic acidC. This purine base pairs with
ThymineD. Deoxyribonucleic acid: contains
genes E. This pyrimidine base pairs with
AdenineF. DNA is copiedG. This purine base pairs with
CytosineH. This pyrimidine base pairs with
GuanineI. 2 strands of DNA twisted togetherJ. Contains a double nitrogen ringK. Contains a single nitrogen ring
RNA VOCAB. – Write these down!
1. Transcription2. Translation3. Codon4. Anti-Codon5. RNA6. rRNA7. mRNA8. tRNA
A. “Ribosomal RNA” makes up ribosome & provides site for protein synthesis
B. “Transfer RNA” carries anti-codon & amino acid (looks like a t)
C. Ribonucleic acid; protein synthesis molecules
D. “Messenger RNA” carries protein synthesis directions to ribosome
E. DNA codes into mRNA molecule F. Anti-codon pairs with codon &
amino acids link togetherG. 3-nucleotide unit of mRNAK. Pairs with codon, attached to
tRNA
DNA Base pairs:
A – T
C – G
• Held together by hydrogen bonds (weak)
• Secured to a sugar-phosphate backbone
DNA ReplicationGoal:
DNA is copied.
Pairs:
A – T
C – G
Steps: 1. DNA “unzips”
2. Complimentary free nucleotides attach to sides of “zipper” to form new DNA strand
Practice Replication Now:
A T C G T G C A T - - - - - - - - -
T A G C A C G T A
Key players in creating protein
1. DNA - contains directions
2. mRNA - carries directions from nucleus through cytoplasm to the ribosome
3. rRNA - binds to the mRNA & uses instructions to assemble AA in order
4. tRNA - delivers amino acid to ribosome for assembly into a protein
Transcription occurs in the NUCLEUS
Steps =1. Double helix
unwinds
2. DNA “unzips”
3. Complimentary free nucleotides attach to sides of “zipper” to form a strand of mRNA
No Thymine is available in RNA, it is replaced by
URACIL (U). (No more T… A – U or U – A)
Translation occurs in the RIBOSOME
Steps = 1. mRNA & tRNAs
carrying amino acids meet at ribosome
2. tRNA triplet anti-codon matches mRNA codon
3. #2 repeats & amino acids chain together until entire mRNA strand is translated into proteinRemember: No Thymine is available in
RNA, it is replaced by URACIL (U). (No more T… A – U or U – A)
From the next 3 slides, pick your favorite
mRNA amino acid chart
1. Practice the translation process and determine the amino acid from these mRNA sequences:
UCAAACCGAGUACAG
My favorite. Time to practice…Practice the translation
process and determine the amino acid from these mRNA sequences:
• UCA• AAC• CGA• GUA• CAG
Practice the translation process and determine the amino acid from these mRNA sequences:
• UCA = SERINE• AAC• CGA• GUA• CAG
Practice the translation process and determine the amino acid from these mRNA sequences:
• UCA = SERINE• AAC =
ASPARAGINE• CGA• GUA• CAG
Practice the translation process and determine the amino acid from these mRNA sequences:
• UCA = SERINE• AAC =
ASPARAGINE• CGA =
ARGININE• GUA• CAG
Practice the translation process and determine the amino acid from these mRNA sequences:
• UCA = SERINE• AAC =
ASPARAGINE• CGA =
ARGININE• GUA = VALINE• CAG
Practice the translation process and determine the amino acid from these mRNA sequences:
• UCA = SERINE• AAC =
ASPARAGINE• CGA =
ARGININE• GUA = VALINE• CAG =
GLUTAMINE
Not my fave, but time to practice…Practice the translation
process and determine the amino acid from these mRNA sequences:
• UCA• AAC• CGA• GUA• CAG
Practice the translation process and determine the amino acid from these mRNA sequences:
• UCA = SERINE• AAC• CGA• GUA• CAG
Practice the translation process and determine the amino acid from these mRNA sequences:
• UCA = SERINE• AAC =
ASPARAGINE• CGA• GUA• CAG
Practice the translation process and determine the amino acid from these mRNA sequences:
• UCA = SERINE• AAC =
ASPARAGINE• CGA =
ARGININE• GUA• CAG
Practice the translation process and determine the amino acid from these mRNA sequences:
• UCA = SERINE• AAC =
ASPARAGINE• CGA =
ARGININE• GUA = VALINE• CAG
Practice the translation process and determine the amino acid from these mRNA sequences:
• UCA = SERINE• AAC =
ASPARAGINE• CGA =
ARGININE• GUA = VALINE• CAG =
GLUTAMINE
Just discovered this one. Time to practice…Practice the translation
process and determine the amino acid from these mRNA sequences:
• UCA• AAC• CGA• GUA• CAG
Practice the translation process and determine the amino acid from these mRNA sequences:
• UCA = SERINE• AAC• CGA• GUA• CAG
Practice the translation process and determine the amino acid from these mRNA sequences:
• UCA = SERINE• AAC =
ASPARAGINE• CGA• GUA• CAG
Practice the translation process and determine the amino acid from these mRNA sequences:
• UCA = SERINE• AAC =
ASPARAGINE• CGA =
ARGININE• GUA• CAG
Practice the translation process and determine the amino acid from these mRNA sequences:
• UCA = SERINE• AAC =
ASPARAGINE• CGA =
ARGININE• GUA = VALINE• CAG
Practice the translation process and determine the amino acid from these mRNA sequences:
• UCA = SERINE• AAC =
ASPARAGINE• CGA =
ARGININE• GUA = VALINE• CAG =
GLUTAMINE
This is the BEST transcription/translation website ever. Try IT
now!http://learn.genetics.utah.edu/content/begin/dna/transcribe/
USE THE mRNA CODE PROVIDED
TO ADD THE COMPLEMENTARY tRNA
U C G
A G C C U G U A G G C A
A U CG A C U A C
mRNA
tRNA AMINO ACID
USE THE mRNA & tRNA TO TRANSCRIBE THE PROTEIN CHAIN
G A C A U C C G U
A C G
A G C C U G U A G G C A
mRNA
tRNA
AMINO ACID
USE THE mRNA & tRNA TO TRANSCRIBE THE PROTEIN CHAIN
A U C
G A C
C G U
U C G
mRNA
tRNA
A G C C U G U A G G C A
AMINO ACID
USE THE mRNA & tRNA TO TRANSCRIBE THE PROTEIN CHAIN
C G U
G A C A U C
mRNA
tRNA
A G C C U G U A G G C A
AMINO ACIDPROTEIN CHAIN
USE THE mRNA & tRNA TO TRANSCRIBE THE PROTEIN CHAIN
C G UA U C
mRNA
tRNA
A G C C U G U A G G C A
AMINO ACIDPROTEIN CHAIN
LET’S REVIEW THE KEY POINTS:
1. Where is DNA located?2. Where does REPLICATION occur?3. The difference between replication & protein synthesis
is ____________________________.4. What molecule chain is formed during transcription?5. Once that molecule is formed from where, to where,
does the molecule travel?6. mRNA is read in 3-nucleotide segments known as
_______.7. Once at the ribosome, the last step of protein
synthesis, _________ occurs.8. ______ has an anti-codon at the base which pairs with
each codon on the mRNA. 9. An amino acid is present at the top of tRNA. Amino
acids begin to link together to form a _____________.
LET’S REVIEW THE KEY POINTS:
1. Where is DNA located? INSIDE THE NUCLEUS2. Where does REPLICATION occur? 3. The difference between replication & protein synthesis is4. What molecule chain is formed during transcription? 5. Once that molecule is formed from where, to where, does the
molecule travel? 6. mRNA is read in 3-nucleotide segments known as ________.7. Once at the ribosome, the last step of protein synthesis,
__________ occurs.8. ____ has an anti-codon at the base that pairs with a codon on
mRNA. 9. An amino acid is present at the top of tRNA. Amino acids
begin to link together to form a ________________.
LET’S REVIEW THE KEY POINTS:
1. Where is DNA located? INSIDE THE NUCLEUS2. Where does REPLICATION occur? INSIDE THE NUCLEUS3. The difference between replication & protein synthesis is4. What molecule chain is formed during transcription? 5. Once that molecule is formed from where, to where, does the
molecule travel? 6. mRNA is read in 3-nucleotide segments known as ________.7. Once at the ribosome, the last step of protein synthesis,
__________ occurs.8. ____ has an anti-codon at the base that pairs with a codon on
mRNA. 9. An amino acid is present at the top of tRNA. Amino acids
begin to link together to form a ________________.
LET’S REVIEW THE KEY POINTS:
1. Where is DNA located? INSIDE THE NUCLEUS2. Where does REPLICATION occur? INSIDE THE NUCLEUS3. The difference between replication & protein synthesis is
REPLICATION IS TO COPY DNA, WHILE PROTEIN SYNTHESIS CREATES RNA & FORM PROTEIN CHAINS.
4. What molecule chain is formed during transcription? 5. Once that molecule is formed from where, to where, does the
molecule travel? 6. mRNA is read in 3-nucleotide segments known as ________.7. Once at the ribosome, the last step of protein synthesis,
__________ occurs.8. ____ has an anti-codon at the base that pairs with a codon on
mRNA. 9. An amino acid is present at the top of tRNA. Amino acids
begin to link together to form a ________________.
LET’S REVIEW THE KEY POINTS:
1. Where is DNA located? INSIDE THE NUCLEUS2. Where does REPLICATION occur? INSIDE THE NUCLEUS3. The difference between replication & protein synthesis is
REPLICATION IS TO COPY DNA, WHILE PROTEIN SYNTHESIS CREATES RNA & FORM PROTEIN CHAINS.
4. What molecule chain is formed during transcription? mRNA5. Once that molecule is formed from where, to where, does the
molecule travel? 6. mRNA is read in 3-nucleotide segments known as ________.7. Once at the ribosome, the last step of protein synthesis,
__________ occurs.8. ____ has an anti-codon at the base that pairs with a codon on
mRNA. 9. An amino acid is present at the top of tRNA. Amino acids
begin to link together to form a ________________.
LET’S REVIEW THE KEY POINTS:
1. Where is DNA located? INSIDE THE NUCLEUS2. Where does REPLICATION occur? INSIDE THE NUCLEUS3. The difference between replication & protein synthesis is
REPLICATION IS TO COPY DNA, WHILE PROTEIN SYNTHESIS CREATES RNA & FORM PROTEIN CHAINS.
4. What molecule chain is formed during transcription? mRNA5. Once that molecule is formed from where, to where, does the
molecule travel? FROM INSIDE THE NUCLEUS THROUGH THE CYTOPLASM TO THE RIBOSOME
6. mRNA is read in 3-nucleotide segments known as ________.7. Once at the ribosome, the last step of protein synthesis,
__________ occurs.8. ____ has an anti-codon at the base that pairs with a codon on
mRNA. 9. An amino acid is present at the top of tRNA. Amino acids
begin to link together to form a ________________.
LET’S REVIEW THE KEY POINTS:
1. Where is DNA located? INSIDE THE NUCLEUS2. Where does REPLICATION occur? INSIDE THE NUCLEUS3. The difference between replication & protein synthesis is
REPLICATION IS TO COPY DNA, WHILE PROTEIN SYNTHESIS CREATES RNA & FORM PROTEIN CHAINS.
4. What molecule chain is formed during transcription? mRNA5. Once that molecule is formed from where, to where, does the
molecule travel? FROM INSIDE THE NUCLEUS THROUGH THE CYTOPLASM TO THE RIBOSOME
6. mRNA is read in 3-nucleotide segments known as CODONS.7. Once at the ribosome, the last step of protein synthesis,
__________ occurs.8. ____ has an anti-codon at the base that pairs with a codon on
mRNA. 9. An amino acid is present at the top of tRNA. Amino acids
begin to link together to form a ________________.
LET’S REVIEW THE KEY POINTS:
1. Where is DNA located? INSIDE THE NUCLEUS2. Where does REPLICATION occur? INSIDE THE NUCLEUS3. The difference between replication & protein synthesis is
REPLICATION IS TO COPY DNA, WHILE PROTEIN SYNTHESIS CREATES RNA & FORM PROTEIN CHAINS.
4. What molecule chain is formed during transcription? mRNA5. Once that molecule is formed from where, to where, does the
molecule travel? FROM INSIDE THE NUCLEUS THROUGH THE CYTOPLASM TO THE RIBOSOME
6. mRNA is read in 3-nucleotide segments known as CODONS.7. Once at the ribosome, the last step of protein synthesis,
TRANSCRIPTION occurs.8. ____ has an anti-codon at the base that pairs with a codon on
mRNA. 9. An amino acid is present at the top of tRNA. Amino acids
begin to link together to form a ________________.
LET’S REVIEW THE KEY POINTS:
1. Where is DNA located? INSIDE THE NUCLEUS2. Where does REPLICATION occur? INSIDE THE NUCLEUS3. The difference between replication & protein synthesis is
REPLICATION IS TO COPY DNA, WHILE PROTEIN SYNTHESIS CREATES RNA & FORM PROTEIN CHAINS.
4. What molecule chain is formed during transcription? mRNA5. Once that molecule is formed from where, to where, does the
molecule travel? FROM INSIDE THE NUCLEUS THROUGH THE CYTOPLASM TO THE RIBOSOME
6. mRNA is read in 3-nucleotide segments known as CODONS.7. Once at the ribosome, the last step of protein synthesis,
TRANSCRIPTION occurs.8. .tRNA has an anti-codon at the base that pairs with a codon
on mRNA. 9. An amino acid is present at the top of tRNA. Amino acids
begin to link together to form a ________________.
LET’S REVIEW THE KEY POINTS:
1. Where is DNA located? INSIDE THE NUCLEUS2. Where does REPLICATION occur? INSIDE THE NUCLEUS3. The difference between replication & protein synthesis is
REPLICATION IS TO COPY DNA, WHILE PROTEIN SYNTHESIS CREATES RNA & FORM PROTEIN CHAINS.
4. What molecule chain is formed during transcription? mRNA5. Once that molecule is formed from where, to where, does the
molecule travel? FROM INSIDE THE NUCLEUS THROUGH THE CYTOPLASM TO THE RIBOSOME
6. mRNA is read in 3-nucleotide segments known as CODONS.7. Once at the ribosome, the last step of protein synthesis,
TRANSCRIPTION occurs.8. .tRNA has an anti-codon at the base that pairs with a codon
on mRNA. 9. An amino acid is present at the top of tRNA. Amino acids
begin to link together to form a PROTEIN (POLYPEPTIDE) CHAIN.