Metabolic Cage

FEEDING – DRINKING MONITORING SYSTEM Cat. No. 5 1 8 0 0

UGO BASILE BIOLOGICAL RESEARCH APPARATUS

INSTRUCTION MANUAL Via G. Borghi 43 21025 COMERIO - Varese, ITALY

FEEDING - DRINKING MONITORING SYSTEM

Cat. No. 5 1 8 0 0

Series No. Mfg. date

Instruction Manual dated December 2006

Revision 1

UGO BASILE - ITALY Contents – Page i 51800 Instruction Manual (Rev. 1)

C O N T E N T S

1 INTRODUCTION.............................................................................................................1

1.1 LIST OF COMPONENTS ...........................................................................................1 1.2 CONNECTING THE HUB TO THE PC..........................................................................1 1.3 CONNECTING A CAGE ............................................................................................1 1.4 SOFTWARE SETUP.................................................................................................1

2 SOFTWARE DESCRIPTION..........................................................................................2

2.1 STARTUP AND FIRST SETUP....................................................................................2 2.2 MODIFYING THE ASSIGNMENT OF THE CAGES...........................................................3 2.3 STRUCTURE OF THE NAVIGATION TREE....................................................................4 2.4 SCALE CALIBRATION AND SETUP OF THE BARS ........................................................6 2.4.1 CALIBRATING THE SCALES 6 2.4.2 SETTING UP THE BARS 8 2.5 TEST SETUP .........................................................................................................9 2.5.1 CREATING A NEW TEST 9 2.5.2 COPYING A TEST 11 2.5.3 DELETING A TEST 11 2.5.4 MODIFYING A TEST 11 2.6 RUNNING A TEST.................................................................................................11 2.7 ANALYSIS OF THE RESULTS..................................................................................14 2.7.1 TABLE 14 2.8 ANALYSIS...........................................................................................................15 2.8.1 FOOD AND DRINK 16 2.8.2 ACTIVITY 17 2.9 FILM...................................................................................................................18

3 FOLDER STRUCTURE ................................................................................................19

4 CUSTOMER SUPPORT ...............................................................................................21

5 ORDERING INFORMATION ........................................................................................21

UGO BASILE - ITALY Contents – Page ii 51800 Instruction Manual (Rev. 1)

FIGURE INDEX Picture 1 – Main Page....................................................................................................... 2 Picture 2 – Assigning a cage............................................................................................. 3 Picture 3 – Description of the id CAGE ............................................................................. 5 Picture 4 - Calibration of the beverage scale .................................................................... 6 Picture 5 - Calibration of the food scale ............................................................................ 7 Picture 6 – Confirmation request for the modified calibration............................................ 7 Picture 7 – Calibration of the activity bars......................................................................... 8 Picture 8 - Test manager................................................................................................... 9 Picture 9 – Setting up a test ............................................................................................ 10 Picture 10 – Saved test setup ......................................................................................... 10 Picture 11 – Request to delete or maintain the results.................................................... 11 Picture 12 – Monitoring System Page............................................................................. 12 Picture 13 – Beginning of a test ...................................................................................... 12 Picture 14 - Test on running mode.................................................................................. 13 Picture 15 - Tabs for viewing the results ......................................................................... 14 Picture 16 – Test results table......................................................................................... 14 Picture 17 – Analysis Tabs.............................................................................................. 15 Picture 18 – Pattern of the Food weight during time ....................................................... 16 Picture 19 - Pane for the management of cursors........................................................... 17 Picture 20 – View of the activity at a given moment........................................................ 17 Picture 21 – Visualizing the film of the test...................................................................... 18

UGO BASILE - ITALY Page 1 51800 Instruction Manual (Rev. 1)

FEEDING-DRINKING MONITORING SYSTEM Cat. 5 1 8 0 0

1 INTRODUCTION

This manual will describe all the hardware and software parts that form the “FEEDING – DRINKING MONITORING SYSTEM” from the physical hardware connection to the setup and use of the software.

1.1 List of components

The system is composed of the following elements:

• N° 1 8-port USB hub • N° 1 user’s manual • N° 1 CD with installation software and drivers

1.2 Connecting the hub to the PC

• Make sure that the Hub is plugged in • Connect the hub to the pc using the USB cable • The hub is ready for use

1.3 Connecting a cage

• Make sure that the cage is plugged in • Connect the cage to the hub with a USB cable • If it is the first connection, Windows will automatically recognize the new

hardware. If so follow the guided procedure in order to install the drivers. Such drivers can be found in the “DRIVER” folder.

1.4 Software setup

• Open the enclosed CD and double click on the “FEED – DRINK MONITOR-ING SYSTEM VER 1_3” folder

• Double click on the “setup.exe” file and follow the instructions as they appear on the screen

• If the drivers have already been installed according to the instructions given in the previous paragraph, the software will be ready for use


2 SOFTWARE DESCRIPTION

2.1 Startup and first setup

From the directory that you selected during the installation procedure launch the file na-med “FEED – DRINK MONITORING SYSTEM VER 1_3.exe” or select “All programs” => “Feed – Drink Monitoring System Ver 1_3” => “Feed – Drink Monitoring System Ver 1_3” from the Windows bar; the window that will appear during startup is shown in picture 1.

Picture 1 – Main Page

Simultaneously a scroll bar will appear on the screen showing that the software is starting up; such procedure consists in checking whether there are any devices connected to the PC and eventually in recognizing them.

During startup, if it is the first time the software is being used or if a new cage has been connected, the window of assignment will be viewed as shown in picture 2:


Picture 2 – Assigning a cage

The unique serial number of the device is displayed on the upper left while on the right is a list of available cages. Assigning the serial number to an ID means that at the next times the software is restarted and if the device is connected, the given ID will be as-signed to it automatically. For example, going back to picture 2 and assuming to select the ID “CAGE_2” and subsequently confirming the values, the name “FDC99998” will be associated only to “CAGE_2”. For the assignment you must:

• Select the cage that you want to associate to the serial number from the drop down menu

• Click on “Assign”

A table with all the IDs of the cages that have already been assigned, along with the de-vice’s serial number next to them, is displayed below. See paragraph 2.2 if you want to make any changes.

Once the ID assignment phase has ended the navigation tree is built. It is structured as shown in picture 1 on the left; in such tree the only active cages will be the assigned ones that are also connected to the system. For the detailed description of the navigation tree see paragraph 2.3.

The menu is at the top of the window: from here it is possible to exit the software (FILE => EXIT), view and modify the assignation of the cages (SETUP => SYSTEM CON-FIGURATION) and consult the manufacturer’s information (ABOUT => INFO).

2.2 Modifying the assignment of the cages

If the assignment isn’t valid, such as if a mistake has been done in assigning a given ID to a serial number or if a new cage is connected in place of another and if there are no more available IDs (since 8 serial numbers are assigned to 8 IDs), it is possibile to change the setup:


• Select “SETUP => SYSTEM CONFIGURATION” from the menu; the window

shown in picture 2 is displayed on the screen • Select the assignment that must be deleted and click on “DELETE ASSIGN-

MENT” • Repeat the procedure as many times as needed • Press the “CONFIRM” button or the “UNDO” button to cancel the whole pro-

cedure • When the CONFIRM button is selected the software shuts down • At the next startup the software will start to recognize the new cage

2.3 Structure of the navigation tree

Once all the cages connected to the system have been assigned to their correct ID, it is possible to configure them and to set up and run the tests.

However at first it is necessary to learn more about the navigation tree (see picture 3). Such tree is useful to visualize all the useful information on the cages that are connected to the system.

Picture 3 – Navigation Tree As seen in this picture a “CAGE” voice is structured as follows:

• CAGE_n o CALIBRATION

ACTIVITY DRINK FOOD

o TEST NAME OF TEST 1


• RESULTS 1 OF TEST 1 • RESULTS 2 OF TEST 1 • ….

NAME OF TEST 2 • RESULTS 1 OF TEST 2 • …

… Such structure is exactly the same for the 8 cages that can be connected to the system. Picture 3 shows the initial situation for one cage, a situation in which no tests have been set up nor done yet. For this reason the structure displayed in this picture does not show voices with test names and results.

A brief description of the pages that appear on the screen by selecting the voices from the tree will be given below.

• CAGE_n: the serial number of the device which has been assigned to the chosen cage is displayed (picture 4)

Picture 3 – Description of the id CAGE

• ACTIVITY: selection of the type of bars of activity of the cages, whether with

22 or 16 sensors (????) (paragraph 2.4.2)

• DRINK: calibration of the beverage scale (paragraph 2.4.1)


• FOOD: calibration of the food scale (paragraph 2.4.1)

• TEST: manager of the tests associated to the cage (paragraph 2.5)

• TEST NAME: name of the test (paragraph 2.6)

• RISULTATI DEL TEST: view and analysis of the test results (paragraph 2.7)

From this point on the manual will give a description of the use of the software, from the scale calibration to the analysis of the test results.

2.4 Scale calibration and setup of the bars

At this stage the setup of the cage connected to the system is defined at its best for the test phase.

2.4.1 Calibrating the scales

The calibration of the scales is fundamental in order to obtain a precise reading of the values; to get a correct calibration it is necessary to have a precise sample weight that will give good results.

• Select the scale of the cage that you want to calibrate from the navigation tree (click on “DRINK” or “FOOD”)

Picture 4 - Calibration of the beverage scale From this moment on it is possible to monitor the test on running mode in the page shown below. The “RUN” button and the cage ID on top flash to warn that the cage is on testing mode.


Picture 5 - Calibration of the food scale

From this moment on it is possible to monitor the test on running mode in the page shown below. The “RUN” button and the cage ID on top flash to warn that the cage is on testing mode. • Type the weight unit measure in the “Drink/Food unit measure” box • Type the value of the sample weight in the “Max engeneering value” box • In absence of weight on the scale click on the “Acquire” button next to the

“Minimum value (V)” box • Put the sample weight on the scale • Click on the “Acquire” button next to the “Maximum value (V)” box • Click on “SAVE”. When the SAVE button is not selected and a voice from the

navigation tree is selected, the following message appears:

Picture 6 – Confirmation request for the modified calibration

Therefore it will be possible to save the changes or to maintain the old values.


When the acquisition is out of the limits that are foreseen for the scales, the value will not be saved and an error message will be displayed.

It is important to note that the values displayed by the scales during testing will be uncor-rect if the scales are not calibrated correctly.

2.4.2 Setting up the bars

The only parameter that needs to be set up for the activity bars is the number of sensors. • Select “ACTIVITY” from the navigation tree; the window displayed below ap-

pears.

Picture 7 – Calibration of the activity bars

• Select the number of leds present in the “activity bar” • Click on the “SAVE” button

It is possible to modify the calibration of the cage at any moment simply by repeating the procedure shown above.


2.5 Test Setup

At least one test must be set up in order to run a test with a cage. By selecting “TEST” from the navigation tree the following window appears:

Picture 8 - Test manager

It is now possible to manage the test setup. The parameters have the following values:

• NAME: test setup name • TEST TIME: duration of the test expressed in “HH:MM:SS” • FOOD: recording of the scale value for food • DRINK: recording of the scale value for beverages • HORIZONTAL ACTIVITY: recording of the horizontal activity • VERTICAL ACTIVITY: recording of the vertical activity • LOG ON EVENT: if selected it records data only when the horizontal position

changes, if not selected it records at a given frequency • LOG FREQUENCY: recording frequency when the “LOG ON EVENT” is not

selected

2.5.1 Creating a New Test

• Assign a name for the test setup in the “NAME” box • Fill out the parameter fields (see picture 10)


Picture 9 – Setting up a test

• Click on the “SAVE” button. The name of the test will appear in the test list and in the navigation tree under “TEST” (see picture 11)

Picture 10 – Saved test setup


The saved set up values will be displayed if a name is selected for the test list. In this case, in order to reset the field and create a new test, click on “NEW”.

2.5.2 Copying a Test

• Select a voice to copy from the list • Assign a new name to the test next to “NAME” • Click on the “SAVE” button

2.5.3 Deleting a Test

• Select the voice that you want to delete from the list of tests • Click on the “DELETE” button • The pop up window asks whether the test results must be overwritten

Picture 11 – Request to delete or maintain the results

2.5.4 Modifying a Test

• Select the test that must be modified from the test list • Modify the parameters that need to be modified • Click on the “SAVE” button; as a further confirmation a window asks if you

want to save the changes

2.6 Running a Test

• Select the test setup that you want to run from the navigation tree; the follow-ing window is displayed:


Picture 12 – Monitoring System Page

• Click on the “RUN” button to start; the following window is displayed:

Picture 13 – Beginning of a test

• Type the animal’s ID, the user’s name and the name of the file of test results in “Test Name” (obligatory field)

• Click on the “OK” button; the name of the test results file is added to the navi-gation tree under the test setup name. Furthermore when the test is on run-ning mode the symbol ”√” appears next to both the test setup name and the test result file


From this moment on it is possible to monitor the test on running mode in the page shown below. The “RUN” button flashes to warn that the cage is on testing mode.

Picture 14 - Test on running mode

The bar over the indicator displays time; the weight indicators display the measure di-rectly in the measuring unit defined during calibration, while the position indicator dis-plays the position of the animal as they keep trace of the number of horizontal and verti-cal movements. In the Event mode recording (“LOG ON EVENT” selected) such data will be viewed only when the position changes either horizontally or vertically; if the recording mode is set on frequency (“LOG ON EVENT” not selected) the data is continually dis-played on video, but the saved data is determined by the chosen frequency.

Even when some fields are not selected during the test setup (for example the “FOOD” field is not of interest and therefore is not selected), the indicators are displayed even if they are not recorded. If the weight values seem out of scale because too much weight has been added or taken out from a scale during the test, the indicator of such scale will start to blink and the in result table it will be saved as “O.o.R.” (Out of Range).

To stop a test click on the “STOP” button or wait for the end of the test; in such case a “TEST COMPLETE” message will be displayed at the end of the test.

In order to exit the software select “FILE => EXIT” from the menu. If there is at least one running test, a window will ask to confirm and all the running tests will stop.

In such case the results will obviously be recorded only for the portion of the test that has been carried out; the same happens when the “STOP” button is selected.


2.7 Analysis of the results

Select the name of the result file from the navigation tree. The analysis section is made up of a series pages that can be accessed to from the Tabs.

Picture 15 - Tabs for viewing the results

All the results can be seen even if the test is still running; obviously in this case it will be only possible to view the results up to that moment, otherwise it will be possible to view the data that appears in the summary table that can be found in the “TABLE” tab (see the next paragraph).

If the test does not foresee any sensors, the relating fields will contain null values.

2.7.1 Table

The first voice that appears by clicking on the result file name in the navigation tree is the “TABLE” tab.

Picture 16 – Test results table


It is a summary in text form of the performance of the test represented by a table; the columns of the table can be defined as follows:

• TIME: time duration expressed in HH:MM:SS:d • FOOD: food weight • DRINK: beverage weight • H. POSITION: horizontal position of the animal • V. POSITION: vertical position of the animal • H. COUNT: number of movements based on the horizontal position • V. COUNT: number of movements based on the vertical position

The values that can be seen in the table are of the same nature of the file created during the testing. This means that by opening such file with software as “EXCEL”, the view will be the same, except for the data related to the STARTING TIME, the ANIMAL NAME and the USER NAME which will appear in the first row of the exported data file.

As for the representation of the positions, the string will be viewed as a series of “0”s and “1”s where ”1” shows where the animal was at a given moment. For example, with a 16 sensor bar this could be a possible string:

0000000011110000

In this way it is easy to understand where the animal was located since such string is a representation of the sides of the cage.

Since it is also possible to view the results of a test that is still running, and therefore to read the data up until that moment, the table can be updated by clicking on the “UP-DATE” button. Such table, in fact, does not modify automatically each time new data is recorded. It is therefore necessary to do a manual update; all the data that can be found in the other analysis pages (see the other tabs), refer to the contents of this table.

It is also possibile to print the table by clicking on the “PRINT” button.

Therefore if this table is not updated, the data from the other pages will not be updated either. If the table displays a completed test, by selecting “DELETE THIS TEST RESULT” the result file of this test will be deleted and the display will go back to the test setup win-dow. It is important to note that deleting the results means that the result file will be com-pletely deleted and the data will be lost forever.

2.8 Analysis

The “ANALYSIS” tab contains three other tabs: “FOOD”, “DRINK” e “ACTIVITY”.

Picture 17 – Analysis Tabs


2.8.1 Food and Drink

The “FOOD” and “DRINK” tabs are absolutely identical. The first displays a graph of the food weight pattern during time, and the latter displays the same thing for beverages.

Picture 18 – Pattern of the Food weight during time

From this graph it is possible to obtain the variation given by the weight in a given lapse of time:

• Select a cursor from the pane located under the graph by clicking on “Point 1” or “Point 2”

• Use the joystick located on the right of the pane in order to move the cursor towards the right or the left and select the time

• Repeat the procedure for the other cursor as well • Click on the “DISTANCE” button in order to obtain the weight variation during

the selected lapse of time

• It is alsoo possibile to export some of the data contained in the graph on a .txt file. Use the cursors as previously shown in order to select two points from the graph. Click on the “ADD POINTS” button; the two selected points are added to the table displayed on the lower right. By selecting the “DELETE POINT” button the point is deleted from the table. Select “EXPORT” in order to save the data on an external file; a default name and a path are assigned. They can both be changed by the user.

• By clicking on the “PRINT” button the graph will be printed as it appears at that given moment


Picture 19 - Pane for the management of cursors

2.8.2 Activity

The “ACTIVITY” tab displays both the horizontal and vertical positions at any moment and the movements done until that moment.

Picture 20 – View of the activity at a given moment

By selecting the desired moment from the “TIME” drop down menu on the lower right side, their positions will be shown in the indicators located below. In this case, as in the weight analysis case, it is possible to export some of the graph’s points on an external file. The procedure is exactly the same as the weight analysis one


• Use the cursors as previously shown in order to select two points from the graph • Click on the “ADD POINTS” button; in the table located on the lower left side the

two added points are displayed • By selecting a point from the table and by clicking on the “DELETE POINT” the

point is deleted from the table • Select “EXPORT” in order to save the data contained in the table on an external

file; a default name and a path are assigned. They can both be changed by the user

By clicking on the “PRINT” button the graph will be printed as it appears at that given moment.

2.9 Film

In the “FILM” tab it is possible to monitor the performance of the test in a specified time period and at a given filming speed.

Picture 21 – Visualizing the film of the test

• Select the starting time in the “STARTING TIME” box • Select the ending time in the “ENDING TIME” box • Select the number of frames per second in the “Frame/Sec”box: this value

shows the number of events that must be visualized every second. This


means that the reproduction does not reflect real time; for example, if the re-cording had been done for example based on events, showing a frame per second, the events would be viewed with a frequency of one second, even if the time lapse from on event to the other might be longer.

• Click on “PLAY”; the play symbol will appear in the navigation tree next to the test result file

• Click on “STOP” to stop viewing. The reproduction automatically stops when it reaches the time specified in the “ENDING TIME” box and a popup message will appear.

3 FOLDER STRUCTURE

By using the software according to the instructions given in the previous chapter, some folders and files are created. It is particularly useful to see the files and folders related to the test results in detail.

The structure of such folders is as follows:

• Main folder o File “config.cfg” o Folder “SERIAL NUMBER 1”

File “calibration.cfg” Folder “TEST”

• File “CONFIGURATION TEST 1” • File “CONFIGURATION TEST 2” • … • File “CONFIGURATION TEST n” • Folder “CONFIGURATION TEST 1”

o File “RESULT 1” o Folder RESULT 1

File “EXPORT 1” File “EXPORT 2”

o File “RESULT 2” o … o File “RESULT m”

• Folder “CONFIGURATION TEST 2” • … • Folder “CONFIGURATION TEST N”

o Folder “SERIAL NUMBER 2” o … o Folder “SERIAL NUMBER 8”

All the folders and the files should not be modified manually because this might not be recognized by the software. The only files that might be of interest for the user are the ones mentioned in the above structure as “RESULT m”; such files, display the results as shown in the table contained in the software (see paragraph 2.7.1). By opening these


files with software such as “EXCEL”, or even simply Windows Note Pad, the test results will be displayed. Below is an example taken from a result file:

STARTING TIME;ANIMAL NAME;USER NAME 10/18/06 16:12:19;mouse 1;Operator 1 Time;Food [gr];Drink [ml];H. Position;V. Position;H.Count.;V.Count. 00:00:00:0;0.2;0.2;00000000000;00000000000;0;0 00:00:05:0;0.2;0.2;00000000000;00000000000;0;0 00:00:10:0;14.8;0.2;00000000000;00000000000;0;0 00:00:15:0;19.8;4.1;00000000000;00000000000;0;0 00:00:20:0;19.8;1.9;00000000000;00000000000;0;0

This is also valid for the exported files which are created by default in the folder named after the result file. In this case, however, the file structure depends on the exported data, and whether this data is related to weight or position:

Time V. COUNT H. COUNT 00:00:35:0 0 0 00:00:35:0 0 0 00:01:20:0 0 0 00:01:20:0 0 0

File exported from the activity graph

Time Drink [ml]

00:00:15:6 130.3 00:00:48:0 131.3

File exported from the beverage weight graph


4 Customer Support

For any further information you may desire concerning the use and/or maintenance of the Feed-Drink Monitor, please do not hesitate to get in touch with our local distributor or with our after sales department at:-

UGO BASILE Viale G. Borghi 43 21025 COMERIO – Varese, ITALY

Phone : +39 0332 744574 Fax : +39 0332 745488

@ e-mail : [email protected]

Before sending any instrument to our factory for repair, we recommend you to get in touch with our service department to obtain a return authorization number (R.A.N.) and shipping/packing instructions.

When returning an instrument, please pack it carefully. We may not be held responsible for damages during transport due to poor packing. Whenever possible, please store the original packing, in case the rare event of returning becomes necessary.

5 ORDERING INFORMATION

51800 Data Acquisition Software for up to 8 cages

INSTRUCTION MANUAL DATED DECEMBER 2006 REVISION 1

N o t e s

_ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _

_ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _

_ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _

_ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _

_ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _

_ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _

_ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _

_ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _

_ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _

_ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _

_ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _

_ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _

_ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _

_ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _

_ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _

_ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _

_ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _

_ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _

_ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _

_ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _

_ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _

_ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _

_ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _

_ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _

_ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _

_ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _

_ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _

_ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _

_ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _

_ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _

N o t e s

_ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _

_ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _

_ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _

_ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _

_ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _

_ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _

_ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _

_ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _

_ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _

_ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _

_ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _

_ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _

_ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _

_ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _

_ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _

_ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _

_ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _

_ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _

_ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _

_ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _

_ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _

_ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _

_ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _

_ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _

_ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _

_ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _

_ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _

_ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _

_ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _

_ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _

N o t e s

_ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _

_ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _

_ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _

_ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _

_ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _

_ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _

_ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _

_ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _

_ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _

_ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _

_ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _

_ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _

_ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _

_ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _

_ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _

_ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _

_ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _

_ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _

_ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _

_ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _

_ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _

_ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _

_ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _

_ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _

_ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _

_ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _

_ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _

_ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _

_ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _

_ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _

Documents

Metabolic Cage