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CHARACTERISTICS OF PROKARYOTIC & EUKARYOTIC CELLS.
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CHAPTER 4CHARACTERISTICS OF PROKARYOTIC & EUKARYOTIC
CELLS
Questions of ImportanceWhat are the 2 basic cell types?
What are the special characteristics associated with Prokaryotic cells?Can you compare and contrast Prok. & Euk. Cells?
What are the structural differences between a Gram Positive and Gram Negative Cell?
Basic Cell TypesProkaryote: single-celled organisms, and ALL are bacteria.
Termed Prokaryotes because they contain NO nucleus (Pro = before, Karyon = nucleus)Prokaryotes not only lack a nucleus but also lack any membrane bound organelles
Prokaryotes are among the smallest of all organismsProkaryotes are thought to be a good deal older than Eukaryotic cells.
Thoughts are that they have been around for over 4 billion years.
Reproduce by a simple process called binary fission
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Eukaryotic Cells
Eukaryote: single-celled or multi-cellular organisms.Eu = true
Karyon = nucleusEuk. cells possess a nucleus as well as various membrane bound organelles
Euk. cells are larger and more complex than Pro. cellsEukaryotic cells reproduce by a process called Mitosis.
Mitosis is similar to binary fissionSome organisms reproduce by budding
Prok. Vs. Euk. cells
Prokaryotic cells have a single circular chromosome; Eukaryotic cells have paired chromosomes
Prokaryotic cells lack histone proteins; Eukaryotic cells have histone proteins
Prokaryotic - cell wall has peptidoglycan; plant and fungal cells have both cellulose and chitin
ONLY Eukaryotic DNA is in a nucleus surrounded by a nuclear membrane
Prokaryotic DNA is in a nuclear region not surrounded by a membrane
BOTH types have a plasma membrane, DNA, Cell Wall (plant cells), Ribosomes, Cytoplasm
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Prokaryotic Classification
Prokaryotic Cells are usually classified according to:Size
ShapeArrangement
Size: generally 0.5 to 2.0 micrometersAlways exceptions
Shapes:
Arrangement:Diplo- 2 bacteria together
Strepto- chain of bacteriaTetrads – 4 cells arranged in a cube
Staphylo- grape like clusters of bacteriaSarcinae- eight cells arranged in a cube
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Prokaryotic Cell Structures
Structurally, bacterial cells consist of the following:1. Cell membrane, usually surrounded by a cell wall
2. Internal cytoplasm with ribosomes, nuclear region, and in some cases granules and/or vesicles
3. Capsules, flagella, and pili (external)
External Structures of the Prokaryotic Cell
The Cell Wall:Lies outside the cell membrane in nearly all bacteria
Two important functions:1. Maintains the characteristic shape
2. Prevents the cell from bursting when fluids flow into the cell by osmosisCell wall contains Peptidoglycan (murein): The single most important component of cell
wallLarge polymer
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Cell MembraneLiving Membrane that serves as a boundary b/w the cell and outside environment
Phospholipid bilayer embedded with proteinsControls what enters and leaves a cell
Monitors envir. for changesAreas associated w/CM
Periplasmic Space: found in Gram Negative bact.Gap b/w the Cell Wall and CM
Area contains peptidoglycanActive area of metabolism and transport
Outer membrane: found in Gram Negative bacteriaOuter most layer, very similar to CM in structure
Not as selective about what passes through itContains LPS (lipopolysaccharide A)
Endotoxin that is released after death of cellDo some research:
Find out what the effects of Lipopolysaccharide A are to the human body.Glycocalyx
Any substance found external to the cell wallThickest structure is a Capsule
Thinnest layer is the slime layerAll bacteria have at least a thin slime layer
CapsuleProtective coat only found on a few types of bacteria
Prevents phagocytosis by WBCPrevents chemical agents from entering the cell
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Slime Layer
Thinner than a capsuleProtects cell from drying out
Helps trap nutrientsCreates “biofilms”
Can help cells bind together or adhere to objectsDental plaque
Distinguishing b/w cells by their cell wall
Gram-positive Bacteria have a relatively thick
layer of peptidoglycan (60-90%)
Gram-negative Bacteria have a more complex
cell wall with a thin layer of peptidoglycan
(10-20%)
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Extras!!Mycoplasm genus – lack a cell wall
vary in shape due to no cell wall
Acid Fast bacteriaCW is 60% lipid and much less peptidogl.
Growth is relatively slow due lipid contentHard to absorb needed nutrients
Structures of Motility
FlagellaExtensions from the cell membrane through the cell wall
Allow the bacterium to move with speed and purposeBacteria can have one or multiple flagella
Monotrichous – one flagellaAmphitrichous- one at each end
Lophotrichous – two or more at each endPeritrichous – all over the surface
Atrichous – No flagella present
Bacterial MovementChemotaxis
Sometimes bacteria move toward or away from substances in their
environment by this nonrandom process
1. Positive chemotaxis: net result is movement towards the attractant
(nutrients)2. Negative chemotaxis: net result is
movement away from the repellent
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Axial Filaments - aka Endoflagella
Present in spirochetesDue to placement b/w outer sheath and cell wall endoflagella cause a spirochete to
rotate like a corkscrewConjugation Pili
Found only in certain groups of bacteriaThese structures attach two cells and furnish a pathway for the transfer of genetic
materialConjugation allows for the genetic variety in bacterium
Creates the issue of antibiotic resistance
Attachment Pili - aka fimbriaeStructures that help bacteria adhere to a surface
Contribute to the pathogenicity of certain typesSome bact. Adhere to RBCs and cause the RBCs to clump = hemoagglutination
Neisseria gonorrhoeae strains w/out rarely cause gonorrhea, pili needed to attach to epithelial cells or sperm
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INTERNAL STRUCTURES
CYTOPLASMSemi-fluid substance inside the CM
70-80% waterConstant metabolic activity
RibosomesConsist of RNA
Sites of protein synthesisUsually found in chains (polyribosomes)
70S (30S and 50S)Eukaryotes are 80S (antibiotics)
Nuclear Region - aka nucleoidWhere DNA is located
Single circular strandSmaller circular DNA (plasmids) also located here
Plasmids are supplemental DNA (circular)Usually passed to other bacteria during conjugation
Inclusions - AKA granulesSmall bodies in the cytoplasm that are not surrounded my membrane
Each contains specific substances such as glycogen (glucose) and polyphosphate (metabolic reactions), enzymes
EndosporesHelps an organism survive
Not a method of reproductionContain very little water
Highly resistant to heat, disinfectants, radiation, acids etc.Chlorine dioxide gas
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Movement of Substances across the membrane
Understanding movement of materials helps us to understand how cells workPolar and nonpolar molecules pass differently
Large vs. small pass differently2 types of transport
PassivePassive TransportNo energy required
Particles move down a gradientHi to Lo conc.
Simple diffusion-Facilitated diffusion-
Osmosis-
Active TransportEnergy Required
Particles move against the gradientLo to Hi conc.
EndocytosisExocytosis
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