Midlands Technical College BIO 225 - Microbiology

Science Department Catalog Course Description: This is a detailed study of microbiology as it relates to infection and the disease processes of the body. Topics include immunity, epidemiology, medically important microorganisms, and diagnostic procedures for identification. Prerequisite(s): BIO 101 or BIO 211 Credit Hours: Lecture: 3.0 Lab: 1.0 Departmental Website: http://www.midlandstech.edu/science D2L Brightspace Login Page: https://elearn.midlandstech.edu Departmental Assistant: Ms. Pam McPherson (mcphersonp@midlandstech.edu) Department Chair: Dr. Geralyne Lopez-de-Victoria (lopezg@midlandstech.edu) Textbook(s): Lecture: Microbiology: Intro.-W/Access Tortora 11th edition,

Pearson Lab: Laboratory Medical Microbiology: Introduction to Skills and Applications. Lema and Corbett, 1st ed. Wiley 2007 A Photographic Atlas for the Microbiology Laboratory. Leboffe, 4th edition Morton

Additional Course Equipment: 1. Lab coats are to be supplied by the student. 2. Protective eye wear is supplied in the lab as needed but students may wish to purchase their own. If deemed necessary. 3. Sharpies and gloves are to be supplied by the students (tight fitting, latex/nitrile) Course Objectives: Upon completion of this course the student will have a working knowledge of basic microbiological principles as related to infectious disease and diagnostic procedures for identification of medically important microorganisms. Course Outcomes and Competencies:

Intended Course Outcome: Students in Biology 225 will learn the basic principles of microbiology in the following areas:

Microscopy Functional Anatomy of Microorganisms Characteristics and Control of Microbial Growth Classification and Identification of Microorganisms Mechanisms of Disease, Host Defenses, and Practical Applications of Immunology

Course Competency: Students will demonstrate understanding of scientific principles and ability to use scientific methodology by employing their knowledge of the fundamentals of microbiology and associated laboratory skills to accomplish the following objectives:

Use aseptic techniques to isolate and maintain a pure culture of the assigned unknown and avoid cross-contamination of bacterial cultures.

Use microscopy and differential staining to identify the physical characteristics of the assigned unknown. Use biochemical tests, selective and differential growth media, and serology to identify the metabolic capabilities of the assigned unknown and use these results to differentiate among pathogenic and non-pathogenic species and identify the unknown.

Performance Measurement Instrument: Students will demonstrate competency by successfully

performing the objectives outlined above, correctly interpreting the results, and identifying their assigned unknown bacterium. Program and course assessment activities are deployed and results collected in accordance with the College’s assessment schedule. Refer to the information in the syllabus regarding the applicability of assessment activity for the current semester.

Course Attendance: Students are expected to be present and ready to work when class begins. Students are expected to attend every class possible. Cuts should be carefully used since there is no such thing as an "excused absence". Students will be allowed to miss twice the number of times a lecture or laboratory section meets per week.

If the lecture meets 3 times per week, 6 absences are allowed. If the lecture meets 2 times per week, 4 absences are allowed. If the laboratory meets once a week, 2 absences are allowed. If the student misses more than 10 minutes of class by either arriving late or leaving early, then the student will be counted as absent, missing fewer than 10 minutes is a tardy. Three tardies count as one absence.

Students adding courses after classes begin are responsible for work covered from the first day of class. All classes missed count as absences.

Please note the following: You are responsible for all material and announcements presented, whether you are present or absent.

Withdrawal: Students may withdraw from a course any time before the last week of classes (see the current semester college calendar, available on the MTC web site, for official dates). Students who wish to withdraw from a course must submit a withdrawal form to records. The date of withdrawal may affect a number of things, including financial aid/ tuition reimbursement, tuition refunds, and course grades. The effective date of withdrawal depends upon the date the withdrawal form is submitted to records. It is the student’s responsibility to be aware of relevant dates, to make an informed decision, and if necessary, to submit withdrawal forms in a timely fashion.

For questions regarding the effect of withdrawal on financial aid or tuition reimbursement students should contact Student Financial Services. Deadlines for tuition refunds may be found on the current semester college calendar, available on the MTC web site, or by calling the cashier’s office.

Students who withdraw before midterm will receive a grade of W. Students who withdraw after midterm and have an overall class average of 60% or greater will receive a grade of W. Students who withdraw after midterm and have an overall class average below 60% will receive a grade of WF, which is calculated as an F.

Grades of W or WF are also assigned when a student exceeds the maximum number of absences allowed in a course. These grades are entered on the final grade roster along with the last date of attendance (LDA). Students should understand that the LDA does not constitute an effective date of withdrawal and should not consider a decision to stop attending class to be equivalent to withdrawal. No Shows: If you register for a course and decide not to attend for any reason, you must complete a drop form and process it through the student Records Office. You will not be automatically purged for non-attendance. If you do not submit a drop form, you will be responsible for course tuition and fees. By not officially dropping the course, you will incur a bill with the college that can only be addressed through the College’s Finance Office. The college’s refund policy and dates are posted each semester.

Effective Spring 2015, the Student Ombudsman’s office will no longer be the initial point of contact for requesting No Shows to be processed. Students who incur a bill must contact the Finance Office. Administrative Drop Requests: A student requesting an Administrative Drop resulting from medical event, death of family member, and other extenuating circumstances experienced while enrolled at Midlands Technical College should be directed to the Student Ombudsman’s office. Our policy dictates a request must be made no later than 30 days after the affected term. Supporting documentation is required and must be received before the request can be processed. Once the request form is received along with supporting documentation, it takes approximately three weeks for processing. As a result of an approved Administrative Drop Request, the student may be granted a refund of tuition and fees.

Military Withdrawal: According to College Procedure 3.10.1, students having to withdraw from college because of Military Deployment (active duty personnel) while enrolled must complete a withdrawal form and submit to the Records Office along with a copy of military orders. Disabilities Statement: The staff of Counseling and Career Services works to ensure that all educational programming and services are accessible to otherwise qualified students with disabilities. If you have a concern regarding the accessibility of websites, instructional materials, online courses and other electronic or information technology please contact Counseling and Career Services. It is the student's responsibility to self-disclose as a student with a disability and to request accommodations prior to beginning a program or course. Please contact the staff of Counseling and Career Services at 803-822-3505 (AC) or 803-738-7636 (BC) or via email at disability@midlandstech.edu if you have any questions or concerns. D2L Brightspace Help:

Online Learning Support Help Desk: Technical questions related to the operation and use of D2L Brightspace can be answered from our Support Help Desk. A response will be provided within one business day. To login: use your MTC e-mail account username and password. (Student Username Example: georgeasmith) (Faculty Username Example: smithg) D2L Brightspace Assistance: Technical questions related to the operation and use of D2L Brightspace can also be answered by leaving a voicemail at (803) 822-3561, or emailing D2LHelp@midlandstech.edu. A response will be provided within one business day. MyMTC Help: For MyMTC log-in issues please call 803-738-7888.

Academic Dishonesty: For more information about academic dishonesty, see the Academic Affairs Student Guidelines and Expectations attached.

If you are suspected of cheating, your instructor will inform you. You may explain or refute the allegation. If your instructor still thinks the charges are founded, you will be referred to the Office of the AVP, SDS. Documentation is submitted to the Office of the AVP, SDS, by your instructor. You will then meet with either Dr. Holloway or Mr. Hayden. After the meeting, you will receive a letter with the sanction grade of zero (0) and any other sanctions deemed appropriate. You will have the right to file an appeal. Once the hearing and the notice of the right to appeal have been completed, the instructor will be notified to apply the sanction grade of zero (0). Course Requirements: Students should understand that they are responsible for all material and announcements given in class/lab regardless of whether they are present or not. Students are not to go in and out of the class during lecture or an exam unless there is an emergency. Students leaving the classroom will be counted tardy for missing any time or absent for missing more than 10 minutes of class time. Students should make arrangements to go to the restroom between classes, not during class. Students who are absent from a class are responsible for all of the work that is done during that class period and for all assignments made during the missed class period. One (1) missed lecture exam may be made up if a legitimate excuse is presented to your lecture instructor. All make-ups will be at the convenience of the lecture instructor. Documented evidence of extenuating circumstances may be required. Missing an examination because of vacations, trips or appointments (non-emergency) will not be considered an extenuating circumstance. Make-up exams may be written (objective, discussion), oral or a combination and may differ significantly in format from those administered at the regular examination period. Students missing the final exam may be assigned the grade of “0” unless the course instructor has received notification within 24 hours following the examination. At the instructors discretion students who contact the instructor prior to an exam may be allowed to take that exam at an alternate time if exceptional reasons exist. Missed laboratory work cannot be made up. This includes quizzes. Final exams are given only during the final exam examination period. They may not be given early. Final exams cannot be exempted or dropped. Final exam grades cannot be curved. Lecture Grading: The lecture grade will be determined by performance on a series of tests. See schedule and instructor for details on material covered, format of the test and specifics of grading. LABORATORY GRADING: I. LABORATORY TECHNIQUE

A. Pour Plate 5 Points B. Aseptic Transfer 5 Points C. Streaking for isolation 5 Points D. Miscellaneous Exercises 5 Points

TOTAL 20 Points

II. LITERATURE AND INTREPRETATION A. Microbe Reports* 30 Points B. Case Studies 45 Points C. Internet Exercise 10 Points

TOTAL 85 Points *Each student will give 2 reports on organisms that will be assigned by the instructor from a selected list. Reports will be both written and oral. A one-page form will be supplied for the written report. The format must be followed. III. LABORATORY PRACTICALS

A. Microscope Practical 40 Points B. Staining Practical 40 Points C. Midterm Practical 325 Points D. Media Practical 40 Points E. Final Practical 325 Points TOTAL 770 Points

All laboratory practicals are departmental. Lab instructors may not drop questions from practicals, may not curve or scale practicals, and may not give any bonus credit questions. IV. IDENTIFICATION OF A BACTERIAL UNKNOWN

A. Morphology and Gram Stain 25 Points A.1.Colonial Morphology (shape, size, margin, elevation, color) - 5 points A.2.Cellular Morphology (shape, size, arrangement) - 6 points A.3.Gram stain reaction - 14 points

B. Identification of Unknown/Biochemical Test 100 Points C.Correct genus – 25 points D.Correct species – 25 points E.Appropriateness of biochemical tests – 25 points F.Correctness of biochemical test reports – 25 points

TOTAL 125 Points

TOTAL POSSIBLE POINTS: 1000

LAB WORK IN THIS COURSE CANNOT BE MADE UP. STUDENTS MAY NOT ATTEND LABS OTHER THAN THEIR OWN. LABORATORY PERFORMANCE DEFICIENCIES (LPDs): Students will be assessed a point penalty, 10 points for each violation, for laboratory deficiencies which are defined as any violation of the performance criteria such as the following: Failure to carry out laboratory procedures as directed:

1. Failure to listen to instructors’ directions and failure to follow these directions in the

performance of laboratory exercises.

2. Failure to carry out procedures in a proper, safe and correct manner.

3. Failure to observance of safe laboratory practices.

4. Failure to complete each laboratory assignment on time.

5. Failure to work well with partners — Each student should do their part in team activities.

6. Failure to keep lab tables clean and disinfected before and after each lab session.

7. Failure to wear lab coats at all lab work sessions.

Failure in proper handling and disposal of infectious material: 1. Failure to use aseptic techniques when working with cultures. 2. Swabs placed back in paper packaging before discarding. 3. Swabs placed anywhere other than discard bags. 4. Tubes to be discarded placed anywhere other than discard test tube racks (such as in the

discard bags). 5. Plates to be discarded placed anywhere other than discard bags. 6. Inability to properly light and use the Bunsen burner.

Students who leave lab without turning off their Bunsen burner, who ignite, melt or damage any fixtures or instruments in any way or who do harm to any person in lab shall be dismissed from lab and required to meet with the department chairman before returning to lab. Absences will be accrued during that time. A 50-point deduction will be made from the final summation of lab points.

1. Failure to adjust the Bunsen burner properly. 2. Cutting off the Bunsen burner at the needle valve (lowest valve on the burner). 3. Failure to cut off the Bunsen burner at the jet valve (gas faucet) on the lab table or improper

storage and use of the microscope. 4. Placing the microscope in backwards (oculars always face out). 5. Any lens other than the lowest power left down. 6. Stage left up. 7. Oil left on the microscope. 8. Oil used on any lens other than the oil immersion lens. 9. Dimmer (rheostat) left on. 10. Switch left on. 11. Proficiency in focusing the microscope at all levels.

Where to put it: Culture tubes Discard racks Reagent ampules Biohazard bags Plate cultures Biohazard bags Slides Biohazard bags Contaminated material (other than tubes) Biohazard bags Paper (includes swab packaging) Trash can Swabs Biohazard bags WRITTEN WORK: All written work done by students in partial fulfillment of the course requirements must be typed. This includes organism reports and class assignments and/or case study reports. Instructors may not accept hand written reports. All MTC students have access to our computer labs and it is required that all students be computer literate. All written work in lecture and/or lab must bare the student’s name, which normally should be placed in the upper right hand corner. No name – no grade. Written work may not be turned in binders or folders. Loose pages may not be accepted. Spelling does count and may be graded.

Unknown morphology and unknown report forms are expected to be hand written since they are done in lab. However, how well the information is reported by the student may be considered in grading. Neatness and completeness do count. Work not turned in on time will be accessed a 10% point penalty per incidence. Faulty may refuse to accept work that is more than one week later than the deadline. Laboratory Policies and Safety Precautions: The laboratory experience is designed to supplement and complement material covered in lecture, and is an integral part of the course. Material may be presented in greater detail in lab than in lecture. The laboratory experience may include dissections, films, demonstrations, experiments, workbook exercises, research and writing assignments, and field trips. The day, time, and location of approved field trips will be held during regularly scheduled lab times. Students will provide their own transportation. The laboratory instructors will give a sufficient amount of information and guidance to allow students to complete each lab, but success in lab primarily involves the individual effort of each student. To maximize learning and to minimize the risk of accidents or injury, the following policies and rules will be observed in science labs: ATTENTION

1. No activity such as eating, drinking, etc. is allowed in the laboratory that may result in contact

with or ingestion of the bacteria.

2. Do not touch the bacteria.

3. Do not sniff the plates.

4. Keep hands away from face.

5. If accidental contact is made, wash the body area immediately and thoroughly with soap and

water and if available wipe with an antiseptic, ex. betadine.

6. If a spill occurs, alert the instructor and follow the spill procedure as directed.

7. Spill kit with instructions is found at the shower area.

8. Be sure to wash hands before leaving the laboratory.

Laboratory exercises must be completed during the assigned lab period unless otherwise designated by your instructor.

Students should read laboratory exercises before coming to class. Do not disturb any equipment or demonstrations that have been set up, until your instructor has told you how to proceed with the assignment. Apply yourself in lab. The exercises are designed to reinforce and expand upon material presented in the lecture. Follow directions carefully. Do not hesitate to ask your lab instructor for clarification of any instructions you do not understand. However, do not expect your lab instructor to simply give you answers to information you should be obtaining through the completion of the various lab exercises. Bring all necessary materials to class, including your text. Do not bring any food or drinks into any science lab. Know where the eyewash station and shower are located in each lab. Report injuries immediately to your lab instructor.

Lab coats and protective eyewear are required in certain labs including microbiology and chemistry. At the end of each lab, leave your work space in good order by discarding waste materials, cleaning and disinfecting your lab table, returning all materials used to their proper place, and sliding your chair back under the table. No visitors are allowed in lab or lecture. Neglect of proper safety procedures will result in the student being required to leave the lab and will be required to meet with the department chair before re-admittance.

Course Grading: See specific instructors for details/modifications. See above for late work/missed work/makeup’s. LECTURE GRADE = 75% of the final grade

Exam 1: ____________ Exam 2: ____________ Exam 3: ____________ Exam 4: ____________

Comprehensive Final Exam: ____________ Sum: _____________

Lecture average = (Sum) / 5 (# of lecture exams) = ___________ Lecture points = (lecture average) X 0.75 = ___________ LABORATORY GRADE = 25% of the final grade Laboratory Practical I (Midterm) (Possible 325 points) Laboratory Practical II (Final) (Possible 325 points) Microscope Practical (Possible 40 points) Stain Practical (Possible 40 points) Media Practical (Possible 40 points) Identification of a Bacterial Unknown (Possible 125 points) Laboratory Technique (Possible 20 points) Microbe Reports (Possible 30 points) Case studies (Possible 45 points) Internet exercise (Possible 10 points) Total points (Possible 1000 points) Lab grade calculations are based on 1000 points. Lab average = Total points /1000 = _____ Lab points = (lab average) X 0.25 = _____ Course Grade: (Lecture points) + (Lab points) = (Course Average) Grading Scale: 90-100 A Superior Work 80-89 B Good Work 70-79 C Average Work 60-69 D Below Average Work 0- 59 F Unsatisfactory Work The faculty, coordinators and Science Department Chair are here to assist you. If you are having any difficulty with your class please talk to your instructor first. You can discuss your concerns with the Science Coordinator and then with the Department Chair, if necessary, after you have addressed your concerns with your instructor. Dr. Geralyne Lopez-de-Victoria is Department Chair and can be reached through Jan Oliver, Departmental Assistant at 822-3548. Dr. Gregory Mancini is Beltline Science Coordinator and can be reached at 738-7660 or in LET 421J. The Airport Science Coordinator is TBA.

Course Topic Outline/Course Calendar with Assignments: Spring/Fall Term

Week Lecture Topic Assigned Reading

Textbook Atlas*

1 Introduction Chapter 1

Chemical Principles (Review Only) Chapter 2

Microscopy (Covered in Lab) Chapter 3

Prokaryotic Cell Chapter 4

2 Microbial Metabolism Chapter 5 pp. 201-208

Microbial Growth Chapter 6 pp. 83-88

3 Control of Microbial Growth Chapter 7

Examination I (Chapters 1,2,4,5,6,7)

4 Microbial Genetics Chapter 8 pp. 109-112

Biotechnology Chapter 9 p. 107,108

5 Classification Chapter 10 p. 107,108

Prokaryotic Microbes: Bacteria Chapter 11 pp. 129-158

6 Eukaryotic Microbes: Fungi, Algae Chapter 12 pp. 159-173

Eukaryotic Microbes: Protozoans, Helminths Chapter 12 pp. 175-200

7 Acellular Microbes: Viruses Chapter 13 pp. 123-128

Examination II (Chapters 8,9,10,11,12,13)

8 Principles of Disease and Epidemiology Chapter 14

Mechanisms of Pathogenicity Chapter 15

9 Nonspecific Defenses Chapter 16 pp. 99-104

10 The Immune Response Chapter 17

The Immune Response Chapter 17

11 Applications of Immunology Chapter 18 pp. 113-121

Immune Disorders Chapter 19 p. 124

12 Antimicrobial Chemotherapy Chapter 20 pp. 89-90

Examination III (Chapters 14,15,16,17,18,19,20)

13 Disease: Skin, Eyes, Nervous System Chapters 21, 22

Disease: Circulatory, Lymphatic, Respiratory Chapters 23, 24

14 Disease: Digestive, Urinary, Reproductive Chapters 25, 26

Examination IV (Chapters 21,22,23,24,24,26)

TBA Departmental Comprehensive Final Examination Chapters 1-26

Final Exams cannot be given early.

*Use Atlas index to check for latest pages.

Summer Term

Week Lecture Topic Assigned Reading

Textbook Atlas

1 Introduction: The Microbial Word Chapter 1

Chemical Principles Chapter 2

Microscopy (Covered in Lab Chapter 3

2 Prokaryotic Cell Chapter 4

Microbial Metabolism Chapter 5 pp. 201-208

3 Microbial Growth Chapter 6 pp. 83-88

Control of Microbial Growth Chapter 7

Examination 1 Chapters 1,2,4-7

Microbial Genetics Chapter 8 pp. 109-112

4 Biotechnology Chapter 9 pp. 107-108

Classification of Microorganisms Chapter 10 pp. 107-108

Prokaryotic Microbes: Bacteria Chapter 11 pp. 129-158

5 Eukaryotic Microbes Chapter 12 pp. 159-200

Acellular Microbes Chapter 13 pp. 123-128

Examination 2 Chapters 8-13

6 Principles of Disease & Epidemiology Chapter 14

Microbial Mechanisms of Pathogenicity Chapter 15

7 Innate Immunity Chapter 16 pp. 99-104

Adaptive Immunity Chapter 17

8 Practical Application of Immunology Chapter 18 pp. 113-121

Immune Disorders Chapter 19 p. 124

Antimicrobial Drugs Chapter 20 pp. 89-90

9 Examination 3 Chapters 14-20

Diseases of the Skin and Eyes Chapter 21

Diseases of the Nervous System Chapter 22

Diseases of the Cardiovascular System Chapter 23

10 Diseases of the Respiratory System Chapter 24

Diseases of the Digestive System Chapter 25

Diseases of the Urinary and Reproductive Systems Chapter 26

Examination 4 Chapters 21-26

Exam Week

Departmental Comprehensive Final

Final Exams cannot be given early.

General Lab Topic Reading

See calendar for specific dates and topics Skills Text Atlas Safety and Introduction to the Microbiology Lab Microscopy & Eukaryotic Organisms (Begin) 1 pp54-67 pp23-26 Microscopy & Eukaryotic Organisms (Finish) 1 pp54-67 pp23-26 Microscopy & Prokaryotic Organisms (Begin) 2 pp54-67 pp23-26 Oil immersion (Begin) Microscopy & Prokaryotic Organisms (Finish) 2 pp54-67 pp43-44 Oil immersion (Finish) Living Organisms (Begin) 3 pp54-67 pp43-44 Application 3 pH pp36-37 Measurement Living Organisms (Finish) 3 pp54-67 pp43-44 Growth Media (Begin) 4 Bacterial Presence (Begin) 5 Growth Media (Finish) 4 Bacterial Presence (Finish) 5 Application 6 Yeast Aseptic Techniques (Begin) 7a, 7b Mini Practical 1 - Microscope 1-3 pp54-67 pp23-26 Aseptic Techniques (Finish) 7a, 7b Streak for Isolation (Begin) 8 p169 Colony Types (Begin) 6 pp1-12 Application 7 Simple Stain p67 p27 Colony Types (Continued) 6 pp1-12 Streak for Isolation (Finish) 8 p169 Gram Stain (Begin) 9 pp67-69 pp35-38 Colony Types (Continued) 6 pp1-12 Isolation quiz plate (Begin) 8 p169 Gram Stain (Continued) 9 pp67-69 pp35-38 Acid-Fast Stain (Begin) 9 pp69-71 pp38-42 Endospore Stain (Begin) 9 pp69-71 pp38-42 Colony Types (Continued) 6 pp1-12 Isolation quiz plate (Finish) 8 p169 Capsule Stain (Begin) 9 pp69-71 pp38-42 Gram Stain (Finish) Acid-Fast Stain (Finish) 9 pp69-71 pp38-42 Endospore Stain (Finish) 9 pp69-71 pp38-42 Application 5 Electrophoresis p106-107 Capsule Stain (Finish) 9 pp69-71 pp38-42 Colony Types (Finish) 6 pp1-12 Bacterial Identification using Media Part I (Begin) 10 pp75,55,62 Application 1 DNA Extraction p105-106 Bacterial Identification using Media Part I (Finish) 10 pp75,55,62 Mini Practical 2 – Stains 9 Review for Midterm Laboratory Practical 1-9 Midterm Laboratory Practical 1-9 (includes applications) No Labs

Bacterial Identification using Media Part II (Begin)

11 p167-168 pp16,18

Biochemical Tests for Gram Negative Bacteria (Begin)

12 pp60,67,80,51,78,79

Unknown assigned (Handout) Bacterial Identification using Media Part II (Finish)

11 p167-168 pp16,18

Biochemical Tests for Gram Negative Bacteria (Finish)

12 pp60,67,80,51,78,79

Tests for Gram Positive Bacteria (Begin) 13 pp50,48,52,71,46 Case studies (Assigned) (Handout) Application 4 pH Indicator Molecule Use Tests for Gram Positive Bacteria (Finish) 13 pp50,48,52,71,46 Additional Chemical Tests (Begin) 14 pp57-58,68-69,72,64 Antibiotic Resistance (Begin) 15 pp89-90 Internet studies assigned (Handout) Parasite slides

Unknown Continued Additional Chemical Tests (Finish) 14 pp57-58,68-69,72,64 Antibiotic Resistance (Finish) 15 pp89-90 Unknown Continued Mini Practical 3 – Media 10-14 Review for Final Laboratory Practical 10-15 Internet studies due Unknown due (No exceptions) Application 2 Protein Concentration Final Laboratory Practical (includes applications) Case studies due

10-15