Gessekai

Hotel Monte Hermana Kobe Amalie (#1-2)

Hotel Monterey Kobe(#1-1)

Tokyu Hands

Hankyu SannomiyaTrain Station

Ikuta Shrine Hall

Hotel Villa Fontaine (#3)

The 73rd Fujihara SeminarInternational Conference

“Molecular Life of Diatoms”July 9. Sun – 13. Thu, 2017,

Ikuta Shrine Hall, Kobe, Japan

Map around the Venue

The Venue & BanquetIkuta Shrine Hall (Ikuta Jinja Kaikan)1-2-1 Shimoyamate-Dori, Chuo-Ku, Kobe, 650-0011Phone: +81 78 391 8765

Award CeremonyClub Gessekai (The Moon Bar) 1-3-8 Shimoyamate-Dori, Chuo-Ku, Kobe, 650-0011Phone: +81 78 331 6540

MLD Kobe2017

Mountain (North)

Ocean(South)

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1F

2F

4F

Reception&

PosterSymposium(4F)

Shrine office

Washroom

Washroom

Gishikiden

Symposium &

BanquetRoom “Fuji” & “Hourai”

Washroom

Washroom

OfficeFrontCafe

Entrance

Resistration desk

Poster(2F)

Washroom

Washroom

Symposium (4F)

Shrine office

The Venue, Ikuta Shrine Hall

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Program Date Sessions Contents

July 9 (Sun)

15:00~ Registration: Registration desk opens @ Ikuta Shrine Hall 1st Floor 18:00~20:00 Mixer @ Ikuta Shrine Hall 2nd Floor Lobby

July 10 (Mon)

Session 1 Opening Remarks and Plenary Lectures 8:40~8:50 1.Opening remark: Yusuke Matsuda 8:50~9:10

2.Introduction of Fujihara Foundation of Science: Executive Director, Hironori Iwase3.Plenary Lecture:

9:10~9:55 Jun Minagawa, “Dissipation of excess light energy for photosynthesis in microalgae”9:55~10:40 Jonathan Zehr, “Single-celled symbiosis in diatoms and haptophytes: implications for

biology, evolution and ecology” 10:40~11:00 Coffee break Session 2 Physiology and Biochemistry – Optimization of light energy and photosynthesis Session Chair: Bethany Jenkins 11:00~11:15 Bernard Lepetit, “Using Lhcx knockout mutants to study photoprotection in

Phaeodactylum tricornutum” 11:15~11:30 Ginga Shimakawa, “Universality and diversity on the protection of photosystem I

against photo-oxidative damage in photosynthetic organisms: the diatom Phaeodactylum tricornutum oxidizes P700 without O2-usage”

11:30~11:45 Vasco Giovagnetti, “Insights into the molecular mechanics of excess energy dissipation in the diatom Phaeodactylum tricornutum”

11:45~12:00 Hiroaki Matsui, “Aquaporins in two marine diatoms, Phaeodactylum tricornutum and Thalassiosira pseudonana –The function of plasma membrane type component”

12:00~12:15 Marianne Nymark, “Downsizing the light harvesting complex through CRISPR/Cas9 gene editing of the chloroplast SRP pathway in marine diatoms”

12:15~14:10 Lunch

Session 3 Evolution – Genome structure and gene component Session Chair: Thomas Mock 14:10~14:40 Debashish Bhattacharya (Invited speaker), “The gene loss diet: stories from two algal

lineages” 14:40~14:55 Tatsuhiro Nomaguchi, “Homoeolog expression bias in allopolyploid oleaginous

marine diatom Fistulifera solaris” 14:55~15:10 Ryoma Kamikawa, “Evolutionary crosstalk between the non-photosynthetic plastid

and the cytosol in Nitzschia” 15:10~15:30 Coffee break Session 4 Ecology and Oceanography – How to find mate and friends Session Chair: Virginia Armbrust 15:30~16:00 Marina Montresor (Invited speaker), “Exploring diatom life histories: from the sea to

the laboratory” 16:00~16:15 Sam De Decker, “Identifying drivers of sympatric speciation in the marine benthic

diatom Seminavis robusta using metabolic analysis and whole-genome resequencing”

16:15~16:30 Bryndan Durham, “New metabolic linkages between diatoms and heterotrophic bacteria revealed through transcriptomics and metabolomics”

16:30~16:45 Shady Amin, “Interactions between a diatom and its bacterial consortium exhibit recognition”

16:45~17:05 Coffee break

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Session 5 Cell Biology – Cell structures and mechanism of motility Session Chair: Assaf Vardi 17:05~17:35 Shigeki Mayama (Invited speaker), “A bridge over troubled water: approaches to

comprehensive understanding of diversified diatoms” 17:35~17:50 Michael Schlierf, “Super-resolution imaging reveals protein-templated patterns for

biosilica formation” 17:50~18:05 Serena Flori, “Chloroplast architecture optimises photosynthesis in diatoms”18:05~18:20 Nicole Poulsen, “Trailins: a new protein family in the adhesive trails of diatoms”18:20~20:20 Poster session (odd #) with snack and beverages @ second floor lobby

July 11 (Tue)

Session 6 Physiology and Biochemistry – Metabolism, Carbon Nitrogen, and Sulphur Session Chair: Kim Thamatrakoln 8:50~9:20 Peter Kroth (Invited speaker), “Studying diatom metabolism in the diatom

Phaeodactylum tricornutum using Molecular Tools” 9:20~9:35 Jared Broddrick, “Quantitative modeling of photoautotrophic growth in P.

tricornutum” 9:35~9:50 Ansgar Gruber, “Nucleotide biosynthesis and transport in diatoms” 9:50~10:05 Kathleen Thume, “Oxidized zwitterionic sulfur species as possible shortcut in the

marine sulfur cycle” 10:05~10:20 Adva Shemi, “A diatom-microzooplankton model system provides novel insights into

the signaling role of DMS/P during prey-predator interactions” 10:20~10:40 Coffee break Session 7 Cell Biology – New molecular approaches to cell physiology and genetics Session Chair: Nils Kröger 10:40~10:55 Guilan Yu, “TALEN-mediated phosphoenolpyruvate carboxylase knock-out mutants in

the diatom Phaeodactylum tricornutum” 10:55~11:10 Amit Sharma, “Biolistic and episome based CRISPR/Cas9 systems; what is the

preferred choice?” 11:10~11:25 Takashi Kadono, “Characterization of Chaetoceros salsugineum-infecting DNA virus

promoter of a replication-associated protein gene in Phaeodactylum tricornutum” 11:25~11:40 Katherine Helliwell, “Novel eukaryotic voltage-gated Ca2+ channels play important

signaling roles in diatoms” 11:40~11:55 Leila Tirichine, “One allele at a time: Extent, relevance and mechanisms of

monoallelic gene expression in the model diatom Phaeodactylum tricornutum” 11:55~12:10 Gust Bilcke, “Physiology and molecular characterization of sex signaling in the

diatom Seminavis robusta” 12:10 ~14:00 Lunch Session 8 Bio, Nano Technology – Mechanism of frustule formation and application Session Chair: Tsuyoshi Tanaka 14:00~14:30 Nils Kröger (Invited speaker), “Learning from diatoms: a synthetic biology for

biominerals” 14:30~14:45 Stephan Brückner, “Towards the understanding of silica-biomineralization:

solid-state NMR spectroscopy, DNP Investigations, and MD simulations of the organic/inorganic interface in diatoms and silica biohybrids”

14:45~15:00 Cathleen Fischer, “Diatom biosilica and carbon replica materials in the adsorption of heavy metals”

15:00~15:15 Syota Katayama, “Branched-chain polyamine induces structural change of diatom bio silica”

15:15~15:35 Coffee break

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Session 9 Ecology and Oceanography – From field to lab Session Chair: Chris Bowler 15:35~16:05 Jodi Young (Invited speaker), “Widespread diversity in diatom RuBisCO and CCMs

hints at co-adaptation to different environments” 16:05~16:20 Senjie Lin, “Transforming dino-genes into Phaeodactylum tricornutum for functional

study” 16:20~16:35 Elena Kazamia, “Investigating the molecular mechanisms and physiology of

siderophore-mediated Fe uptake in Phaeodactylum tricornutum” 16:35~16:50 Chana Kranzler, “The interplay between diatom-virus dynamics and nutrient

availability” 16:50~17:10 Coffee break Session 10 Evolution – Adaptation and divergence Session Chair: Leila Tirichine 17:10~17:40 Thomas Mock (Invited speaker), “The genome of Fragilariopsis cylindrus: The

evolution and significance of diverged alleles for adaptation to variable environmental conditions”

17:40~17:55 Akira Kuwata, “Biology of a picoeukaryotic phytoplankton, Parmales (Bolidophyceae), a sister group of diatoms”

17:55~18:10 James Raymond, “The ice-binding proteins of an Antarctic sea ice diatom”18:10~18:20 Remark from Dr. Takao Sekiya, Selection Committee, The Fujihara Seminar 18:20~20:20 Poster session (even #) with snack and beverages @ second floor lobby

July 12 (Wed)

Session 11 Bio, Nano Technology – Strike a mine of diatoms Session Chair: Atle Bones 8:50~9:20 Tomoko Yoshino (Invited speaker), “Single cell analysis of diatoms based on

microcavity array system” 9:20~9:35 Nathalie Pytlik, “Biological gold nanoparticle production by the diatom

Stephanopyxis turris and in vivo SERS analyses” 9:35~9:50 Tsuyoshi Tanaka, “Genetic engineering of marine oleaginous diatoms for improved

biofuel production” 9:50~10:05 Thomas Kiran, “Diatom biorefinery: A promising approach towards sustainable

bioactive molecules and biofuel production” 10:05~10:25 Coffee break

Session 12 Physiology and Biochemistry – Acclimation to environment, major and minor nutrients

Session Chair: Peter Kroth 10:25~10:55 Harriet Alexander (Invited speaker), “Combining in situ and culture-based

approaches to characterize the physiological ecology of blooming and sinking diatoms”

10:55~11:10 Nanae Kimura, “Characterization of phosphate uptake mechanism in the marine diatom, Phaeodactylum tricornutum”

11:10~11:25 Andrew Allen, “New perspectives on the functional and ecological genomics of iron limitation in marine diatoms”

11:25~11:40 Assaf Vardi, “Chloroplast redox dynamics under fluctuating light regimes controls cell fate decisions in marine diatoms”

11:40~11:55 Yoshinori Tsuji, “Functional analysis of the luminal θ-carbonic anhydrase in Phaeodactylum tricornutum: its possible role in coordinating CO2 fixation and photosynthetic electron transport”

11:55~12:15 Coffee break Group Photo in front of Ikuta-Shrine

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Free Time Enjoy Kobe and Kansai area *Please remove poster before Banquet

Banquet 18:30~20:30 20:30~0:00

Dinner @ Ikuta Shrine Hall 4th Floor Conference room Award Ceremony @ Club Gessekai (The Moon Bar)

July 13 (Thr)

Session 13 Cell Biology – Signals and Stress responses Session Chair: Angela Falciatore 8:50~9:20 Benjamin Bailleul (Invited speaker), “Measuring photosynthesis in microalgal

mixtures: application to interactions between diatoms and dinoflagellates” 9:20~9:35 Shiri Graff van Creveld, “Redox regulation of cell fate decisions in diatom’s response

to infochemicals” 9:35~9:50 Avia Mizrachi, “Phenotypic variability in response to oxidative stress unveils a link

between chloroplast redox dynamics and cell fate in marine diatoms” 9:50~10:05 Mayu Nakagawa, “Potential role of adenylyl cyclases as CO2 sensor in the marine

diatom Phaeodactylum tricornutum” 10:05~10:20 Mariella Ferrante, “Gene expression changes in diatoms in response to external

stimuli” 10:20~10:40 Coffee break Session 14 Genomics and Molecular tools for future MLD Session Chair: Mariella Ferrante 10:40~11:10 Chris Bowler (Invited speaker), “Towards a holistic view of diatom evolution, ecology,

and biogeochemical roles in the contemporary ocean” 11:10~11:40 Hiroyuki Ogata (Invited speaker), “Towards unveiling the pivotal roles of viruses in

marine ecosystems” 11:40~13:30 Lunch Session 15 Genomics and Molecular tools for future MLD Session Chair: Yusuke Matsuda 13:30~13:45 Cheuk Man Yung, “In situ interaction between Minidiscus variabilis and

Planctomycete sp. in the coastal ocean” 13:45~14:00 Amanda Hopes, “Advances in CRISPR-Cas in Diatoms and Beyond” 14:00~14:15 Carolina Río Bártulos, “Genome editing in the diatom Phaeodactylum tricornutum,

methods and applications” 14:15~15:00 The last plenary lecture

Virginia Armbrust, “Uncovering the hidden worlds of marine diatoms”

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List of Poster Presentation

# Name Title

P1 Shinya Sato Comparative genomics in two closely related but morphologically distinct clones of centric diatom Hydrosera

P2 Noriaki Nakamura Comparison of organellar genome between opposite mating types of Pseudostaurosira trainorii

P3 Hiroaki Imoda Comparative genome and transcriptome analyses of diatoms for understanding the molecular mechanism of the red tide

P4 Ute Postel Molecular Ecology and Microevolutionary Genomics of Fragilariopsis kerguelensis, a main silicate sinker of the Southern Ocean

P5 Bethany Jenkins Effects of CO2 on physiology and global gene expression in three Thalassiosira diatoms

P6 Robert Lampe Divergent gene expression among phytoplankton taxa in response to upwelling

P7 Ryan Groussman Diel Transcriptional Patterns of Eukaryotic Phytoplankton in the North Pacific Subtropical Gyre

P8 Rachelle Lim Investigating diatom diversity and interactions at the North Pacific transition zone chlorophyll front

P9 Kei Kimura Dynamics of Chaetoceros tenuissimus and its infectious viruses for five years in Hiroshima Bay

P10 Yuji Tomaru Optimum environmental conditions for virus mediated diatom cell lysis are highly diverse among virus species and isolates

P11 John Perez-Coca Revision of the genus Hyalosynedra (Bacillariophyta: Fragilariaceae), with the description of two new species

P12 Sung-Min An Temporal composition change of benthic diatoms assessed by metabarcoding approach in the west coast of Yellow Sea

P13 Xin Lin Insight from coding and noncoding RNA transcriptome: the acclimation of the model diatom Phaeodactylum tricornutum to ocean acidification

P14 Charlotte Walker Characterisation of diatom-like silicon transporters in coccolithophores

P15 Tore Brembu Dynamic responses to silicon in Thalasiossira pseudonana - Identification, characterisation and classification of signature genes and their corresponding motifs

P16 Ya-Yun Chien Identification and characterization of cell wall associated silica affinity protein of Phaeodactylum tricornutum

P17 Shinya Yoshikawa Morphological and transcriptome analysis in cell wall formation of Triparma laevis

P18 Antoine Hoguin Evolutionary history of DNA methylation: dissecting the role of DNA methyltransferases in Phaeodactylum tricornutum

P19 James Russell RNA-sequencing in model diatoms: insights into frustule shape and patterning

P20 Junrong Liang Genome-wide alternative polyadenylation profiles in the diatom Thalassiosira pseudonana

P21 Justin Ashworth Is there coherent [gene] regulatory similarity across diatom species?

P22 Liangliang Kong Molecular and functional analyses of CTR-type copper transporters in an oceanic diatom, Thalassiosira oceanica

P23 Maxine Tan Nitrate sensing with a model pennate diatom, Phaeodactylum tricornutum

P24 Monia Russo Functional study of a lipolytic enzyme in Phaeodactylum tricornutum using CRISPR/Cas9 system

P25 Kristofer Gomes Subcellular proteomics for determining Fe-limited remodeling of plastids in Thalassiosira pseudonana

P26 Valeria Sabatino Lipoxygenase gene silencing in the diatom Pseudo-nitzschia arenysensis reduces oxylipin levels and affects cell growth

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# Name Title

P27 Remo Sanges The Pseudo-nitzschia multistriata genome has been fully sequenced and is now accessible through a dedicated genome browser

P28 Assaf Gal Silicification in Thalassiosira pseudonana imaged with cryoSEM

P29 Gianluca Dell`Aquila Diatoms and their response to phosphate limitation

P30 Camilla Borgonuovo Looking for the molecules mediating sex in the planktonic diatom Pseudo-nitzschia multistriata

P31 Yuichiro Takahashi Photosystem I complex assembly apparatus

P32 Hisashi Ito Chlorophyll c1 synthesis from chlorophyll c2

P33 Takako Ogawa Measurement of CO2 concentrating mechanism by chlorophyll fluorescence in the cyanobacterium Synechocystis sp. PCC 6803

P34 Shin-Ichiro Ozawa Configuration of nine light-harvesting chlorophyll a/b complexes I in photosystem I supercomplex in the green alga Chlamydomonas reinhardtii

P35 Angela Falciatore Different LHCX-related quenching sites control short- and long-term high light acclimation in the marine diatom Phaeodactylum tricornutum

P36 Hung-Yun Lin From the study on the induction of phosphate starvation responses in the marine diatom Phaeodactylum tricornutum and the subsequent development of a high efficiency diatom expression system

P37 Lee-Kuo Kang Identification and transcriptional analyses of nitrate transporter gene (Nrt2) family among Skeletonema species

P38 Marianne Jaubert Deciphering the diatom phytochrome photoreceptor function in the marine environment

P39 Chao-Cheng Cho Genome mining and biochemical characterization of ADP-ribose processing proteins from Phaeodactylum tricornutum

P40 Zhen-Guo Oh Towards a biochemical characterization of the diatom pyrenoid

P41 Ryosuke Okubo Novel pyrenoidal components critical for photosynthesis in the marine diatoms

P42 Kensuke Nakajima An efficient HCO3- transport system through plasma and plastidic membranes

in the marine diatom, Phaeodactylum tricornutum

P43 Martin Wustmann Chitin synthase in Thalassiosira pseudonana and implications for other diatoms

P44 Rui Yamasaki Analysis of triacylglycerol metabolism in a marine centric diatom, Chaetoceros gracilis

P45 Yi-Ling Lin The development of a type III promoter for gene silencing applications to increase oil content in Phaeodactylum tricornutum

P46 Yasuhiro Kashino Advantageous characteristics of the diatom Chaetoceros gracilis as a sustainable biofuel producer

P47 Natsuko Inoue-Kashino Outdoor cultivation of a marine centric diatom, Chaetoceros gracilis

P48 Yu-Heng Cheng Discussion on the relationship between iron storage system and biomagnetism in Phaeodactylum tricornutum

P49 Shao-Chieh Yen The identification and characterization of spermidine synthase in the marine diatom Phaeodactylum tricornutum

P50 Tomomi Nonoyama Functional analysis of microalgal oil body-associated proteins identified from oleaginous diatom Fistulifera solaris JPCC DA0580

P51 Seungbeom Seo Phaeodactylum tricornutum overexpressing phosphoenolpyruvate carboxylase showed the enhanced photosynthetic productivity

P52 Kansei Yamagishi Characterization of putative thylakoidal anion channels in the marine diatom, Phaeodactylum tricornutum

P53 Hanhua Hu 3-hydroxyisobutyryl-CoA hydrolase involved in isoleucine catabolism regulates triacylglycerol accumulation in Phaeodactylum tricornutum

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# Name Title

P54 Yangmin Gong Acetylome profiling reveals extensive lysine acetylation of plastidial enzymes involved in fatty acid synthesis and activation in the model diatom Phaeodactylum tricornutum

P55 Hermanus Nawaly Localization θ-carbonic anhydrases in marine diatoms and their environmental responses

P56 Berne Nicolas Photosynthetic activity of Thalassiosira pseudonana in anoxic condition

P57 Fengyang Sui Toxicological effects of mercury and copper of freshwater diatom Halamphora veneta (Kützing) Levkov

P58 Carly Moreno Transcriptomic response to iron and light limitation in the polar diatom, Fragilariopsis kerguelensis

P59 Kentaro Ifuku Genome Analysis and Genetic Engineering in the Diatom Chaetoceros gracilis

P60 Hideya Fukuzawa Production of ricinoleic acid-containing monoestolide triacylglycerides in an oleaginous diatom, Chaetoceros gracilis

P61 Yukine Shiraishi Development of genetic transformation systems for the diatoms Nitzschia sp. and Achnanthes sp.

P62 Yumi Watanabe Development of endogeneous promoters driving high-level expression of introduced genes in the model diatom Phaeodactylum tricornutum

P63 Masao Adachi Nuclear transformation of the diatom Phaeodactylum tricornutum using PCR-amplified DNA fragments by microparticle bombardment

P64 Atle Bones TBA

P65 Damian Pawolski In vitro morphogenesis of hierarchically porous silica patterns using diatom biomolecules

P66 Kazuo Umemura Observation of diatom cell behaviors by an optical microscopy using a microchamber

P67 Anggara Mahardika Raman spectroscopy for monitoring enzymatic reaction of immobilized enzyme on diatom Thalassiosira pseudonana silica cell wall

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