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Molecular Cloning and Expression of Three Polygalacturonase cDNAs from theTarnished Plant Bug, Lygus lineolarisAuthor(s): Margaret L. Allen and Jeffrey A. MertensSource: Journal of Insect Science, 8(27):1-14. 2008.Published By: University of Wisconsin LibraryDOI: http://dx.doi.org/10.1673/031.008.2701URL: http://www.bioone.org/doi/full/10.1673/031.008.2701

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Molecular cloning and expression of threepolygalacturonase cDNAs from the tarnished plantbug, Lygus lineolaris

Margaret L. Allen1,a and Jeffrey A. Mertens2,b

Abstract

Lygus lineolaris

Llpg1

Keywords:Abbreviations: Lygus lineolaris

Correspondence:Received: Accepted: Published:Copyright:

ISSN:Cite this paper as:

Lygus lineolaris Journal of Insect Science

Introduction

Phaedon cochleariae

Sitophilus oryzae

Lygus lineolaris

Lygus

Lygus

Lygus

Material and MethodsLygus lineolaris

L. lineolaris

Nucleic acid (RNA and DNA) extraction

Library construction

RACE and full-length cloning

Sequencing and Analysis

Semiquantitative RT-PCR

L. lineolaris

Figure 1 Lygus lineolaris

L. lineolaris

Table 1Lygus lineolaris

Gene or EST Primer description Location Sequence (5 -3 )Llpg1Llpg1Llpg1Llpg1Llpg1Llpg1Llpg1Llpg1Llpg2Llpg2Llpg2Llpg2Llpg2Llpg2Llpg2Llpg3Llpg3Llpg3Llpg3Llpg3Llpg3

Results

Llpg2Llpg3

Llpg1

Llpg1

Llpg1 Llpg2 Llpg3

Figure 2 L. lineolaris

L. lineolaris

L. lineolaris

A. nigerL. lineolaris

A. niger A. aculeatus

Figure 2

Figure 2

Table 2

Protein Accession Nucleotide Accession Length (aa) Species Taxon SpeciesBradyrhizobium japonicum Bradyrhizobium japonicum

Caldicellulosiruptor saccharolyticus Caldicellulosiruptor saccharolyticusClostridium phytofermentans Clostridium phytofermentans

Enterococcus faecium Enterococcus faeciumFlavobacterium johnsoniae Flavobacterium johnsoniae

Aspergillus fumigatus Aspergillus fumigatusAspergillus niger Aspergillus nigerAspergillus niger Aspergillus niger

Aspergillus niger Aspergillus nigerCochliobolus carbonum Cochliobolus carbonum

Colletotrichum lindemuthiana Colletotrichum lindemuthianaFusarium graminearum Fusarium graminearum

Kluyveromyces wickerhamii Kluyveromyces wickerhamiiPenicillium griseoroseum Penicillium griseoroseumPenicillium janthinellum Penicillium janthinellumPenicillium olsonii Penicillium olsonii

Penicillium olsonii Penicillium olsoniiPhaedon cochleariae Phaedon cochleariae

Sitophilus oryzae Sitophilus oryzaeMeloidogyne incognita Meloidogyne incognitaArabidopsis thaliana Arabidopsis thaliana

Brassica napus Brassica napusBrassica rapa rapa Brassica rapa rapa

Glycine max Glycine maxOryza sativa Oryza sativa

Phleum pratense Phleum pratensePyrus communis Pyrus communis

Rubus idaeus Rubus idaeusZinnia elegans Zinnia elegans

L. lineolaris

Meloidogyne incognita

S. oryzae P. cochleariae

Llpg

Llpg

L. lineolaris

Llpg

Discussion

L. lineolaris

Figure 3

L.lineolaris

S. oryzae

Llpg

A. niger

L. lineolaris

L. lineolaris

L. lineolaris

Figure 4 Lygus lineolaris

Llpg1 Llpg2

L. lineolaris

A. niger

L. lineolaris

L. lineolaris

L. lineolaris

S. oryzae

S.oryzae

P. cochleariae

L. lineolaris

Figure 4 Llpg3Llpg1 Llpg2 Llpg3

Lygus

Lygus

L.lineolaris

L. lineolaris

L. lineolaris

Llpg3

L. hesperus

L. hesperus L. lineolaris

L. hesperus L.lineolaris

Lygus

L. lineolaris

Lygus

L. lineolaris

Lygus

Disclaimer

Acknowledgements

L. lineolaris

References

LygusFood Research International

Lygus hesperus L. lineolaris

Journal of Entomological Science

LyguslineolarisGenetics and Molecular Research

Nucleic Acids Research

Aspergillus niger

Journal of Biological Chemistry

Proceedings of the National Academy of Science, US A

Journal of Molecular Biology

Journal of Insect Physiology

Aspergillus aculeatus

Journal of Molecular Biology

Lygushesperus L. lineolarisJournal of Entomological Science

Annals of Applied Biology

Diaprepes abbreviatus ComparativeBiochemistry and Physiology

Plant Physiology

endo

European Journal of Entomology

Phaedon cochleariae InsectBiochemistry and Molecular Biology

Entomologia Experimentalis et Applicata

Nucleic AcidsResearch

Canadian Journal of Plant Pathology

Process Biochemistry

Aspergillus tubingensisEuropean Journal of Biochemistry

Applied andEnvironmental Microbiology

Briefings in Bioinformatics

Annales EntomologiciFennici

Schizaphis graminumJournal of Insect Physiology

Protein Engineering

Journal of Biological Chemistry

MolecularPlant and Microbe Interactions

Methods inMolecular Biology

Archives of Insect Biochemistry and Physiology

Journal of Insect Science

Sitophilus oryzae InsectBiochemistry and Molecular Biology

Journal of Biological Chemistry

Lygushesperus Journal of Economic Entomology

Lygus hesperusJournal of Insect Physiology

Aspergillus niger

Journal of Biological Chemistry

Biology of the plant bugs (Hemiptera:Miridae): pests, predators, opportunists