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C O R R I G E N D U M
Monitoring intracellular redoxconditions in the endoplasmicreticulumof livingyeastsMarizela Delic, Diethard Mattanovich & Brigitte Gasser
Department of Biotechnology, University of Natural Resources and Applied Life Sciences (BOKU), Vienna, Austria
Final version published online 23 June 2010.
DOI:10.1111/j.1574-6968.2010.02028.x
We have been notified by Dr Remington, University of Oregon, that in Delic et al. (2010), Eqn. (3) needs a correction factor to compensate for measuring
the second fluorescence at an excitation wavelength different to the isosbestic point.
Corrected term :Rcorr
1� Rcorr¼ F � Fox
Fred � F
Ioxl2
Iredl2
� �ð3aÞ
where I are the fluorescence intensities at excitation wavelength l2 (here 395 nm) of fully oxidized and reduced cells. Consequently the corrected term
Rcorr/(1�Rcorr) is then used for calculating reduction potentials following Eqn. (3).
Eo0 ¼ Eo0roGFP � 29:6 mV� log
Rcorr
1� Rcorrð3Þ
The corrected reduction potentials of the published data are summarized in Table 1.
Reference
Delic M, Mattanovich D & Gasser B (2010) Monitoring intracellular redox conditions in the endoplasmic reticulum of living yeasts.
FEMS Microbiol Lett 306: 61–66.
Table 1. Determined reduction potentials with corrected values
Published reduction
potentials (mV)
Corrected reduction
potentials (mV)
roGFP1 cytosol � 295 � 303
roGFP1_iE ER � 236 � 242
roGFP1_iL ER � 229 � 235
FEMS Microbiol Lett 309 (2010) 114c� 2010 Federation of European Microbiological SocietiesPublished by Blackwell Publishing Ltd. All rights reserved
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