C O R R I G E N D U M

Monitoring intracellular redoxconditions in the endoplasmicreticulumof livingyeastsMarizela Delic, Diethard Mattanovich & Brigitte Gasser

Department of Biotechnology, University of Natural Resources and Applied Life Sciences (BOKU), Vienna, Austria

Final version published online 23 June 2010.

DOI:10.1111/j.1574-6968.2010.02028.x

We have been notified by Dr Remington, University of Oregon, that in Delic et al. (2010), Eqn. (3) needs a correction factor to compensate for measuring

the second fluorescence at an excitation wavelength different to the isosbestic point.

Corrected term :Rcorr

1� Rcorr¼ F � Fox

Fred � F

Ioxl2

Iredl2

� �ð3aÞ

where I are the fluorescence intensities at excitation wavelength l2 (here 395 nm) of fully oxidized and reduced cells. Consequently the corrected term

Rcorr/(1�Rcorr) is then used for calculating reduction potentials following Eqn. (3).

Eo0 ¼ Eo0roGFP � 29:6 mV� log

Rcorr

1� Rcorrð3Þ

The corrected reduction potentials of the published data are summarized in Table 1.

Reference

Delic M, Mattanovich D & Gasser B (2010) Monitoring intracellular redox conditions in the endoplasmic reticulum of living yeasts.

FEMS Microbiol Lett 306: 61–66.

Table 1. Determined reduction potentials with corrected values

Published reduction

potentials (mV)

Corrected reduction

potentials (mV)

roGFP1 cytosol � 295 � 303

roGFP1_iE ER � 236 � 242

roGFP1_iL ER � 229 � 235

FEMS Microbiol Lett 309 (2010) 114c� 2010 Federation of European Microbiological SocietiesPublished by Blackwell Publishing Ltd. All rights reserved

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