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![Page 1: MT-1732products.moravek.com/DataSheets/MT1732DS.pdf · MT-1732 Hypoxanthine, [8-3H]- Lot 212-131-009-A-20091216-DG A) All chromatograms were run using the HPLC method described on](https://reader036.pdfslide.net/reader036/viewer/2022071001/5fbe5b435552cf28e6780309/html5/thumbnails/1.jpg)
![Page 2: MT-1732products.moravek.com/DataSheets/MT1732DS.pdf · MT-1732 Hypoxanthine, [8-3H]- Lot 212-131-009-A-20091216-DG A) All chromatograms were run using the HPLC method described on](https://reader036.pdfslide.net/reader036/viewer/2022071001/5fbe5b435552cf28e6780309/html5/thumbnails/2.jpg)
MT-1732
Hypoxanthine, [8-3H]-
Lot 212-131-009-A-20091216-DG
A) All chromatograms were run using the HPLC method described on the Product Data Sheet.
Concentrations and volumes:
Standard solution concentration was 1.0 mg/ml.
Hypoxanthine, [8-3H]- concentration was 0.2 mCi/ml. Volume of standard alone injection was 1.0 µl.
Volume of Hypoxanthine, [8-3H]- alone injection was 1.0 µl.
Co-injection solution consisted of 1.0 µl Hypoxanthine, [8-3H]- + 1.0 µl standard.
Volume of co-injection was 2.0 µl.
Volume of blank injection was 1.0 µl.
B) Mass spectrometry – Positive mode C) NMR
![Page 3: MT-1732products.moravek.com/DataSheets/MT1732DS.pdf · MT-1732 Hypoxanthine, [8-3H]- Lot 212-131-009-A-20091216-DG A) All chromatograms were run using the HPLC method described on](https://reader036.pdfslide.net/reader036/viewer/2022071001/5fbe5b435552cf28e6780309/html5/thumbnails/3.jpg)
MT-1732Hypoxanthine, [8-3H]-
Lot 212-131-009-A-20091216-DG
Chromatogram Info:
File Name : L:\int21298 File Created : 4/1/2014 1:49:01 PM
Origin : Acquired, Acquisition started 9/12/2011 11:56:43 AM Acquired Date : 9/12/2011 12:36:42 PM
Project : Test By : Administrator
Method : Unit2-40minrun By : Administrator
Description : Radiochemical trace of 3H material alone
Created : 6/16/2007 8:19 AM Modified : 4/1/2014 2:06 PM
Column : Detection : Radiochemical
Mobile Phase : Temperature :
Flow Rate : Pressure :
Note :
[min.]Time
10 20 30 40
[mV]
Volta
ge
0
200
400
600
3.6
1
8.7
2
9.7
3
10.2
4
10.8
5
11.1
6
L:\ int 21298 - Unit 2 Radio
4/1/2014 2:07 PM Page 1 of 2Chromatogram L:\int21298.PRM
![Page 4: MT-1732products.moravek.com/DataSheets/MT1732DS.pdf · MT-1732 Hypoxanthine, [8-3H]- Lot 212-131-009-A-20091216-DG A) All chromatograms were run using the HPLC method described on](https://reader036.pdfslide.net/reader036/viewer/2022071001/5fbe5b435552cf28e6780309/html5/thumbnails/4.jpg)
Result Table (Uncal - L:\int21298 - Unit 2 Radio)
CompoundName
Reten. Time[min]
Area[mV.s]
Height[mV]
Area[%]
Height[%]
Efficiency[th.pl]
Eff/l[t.p./m]
Symmetry/Tailing[-]
Response Factor
Resolution[-]
1 3.64 179.923 10.30 0.99 1.51 1348.21 26964.29 1.67
2 8.71 17966.207 664.54 98.54 97.63 3369.06 67381.27 3.27 10.2
3 9.75 16.774 1.86 0.09 0.27 82232.22 1644644.41 2.06 2.8
4 10.25 19.266 1.56 0.11 0.23 66773.08 1335461.66 4.06 3.4
5 10.77 10.096 1.22 0.06 0.18 44652.67 893053.47 1.86 2.9
6 11.10 40.863 1.21 0.22 0.18 6396.55 127931.08 2.94 0.9
Total 18233.130 680.69 100.00 100.00
4/1/2014 2:07 PM Page 2 of 2Chromatogram L:\int21298.PRM
![Page 5: MT-1732products.moravek.com/DataSheets/MT1732DS.pdf · MT-1732 Hypoxanthine, [8-3H]- Lot 212-131-009-A-20091216-DG A) All chromatograms were run using the HPLC method described on](https://reader036.pdfslide.net/reader036/viewer/2022071001/5fbe5b435552cf28e6780309/html5/thumbnails/5.jpg)
MT-1732Hypoxanthine, [8-3H]-
Lot 212-131-009-A-20091216-DG
Chromatogram Info:
File Name : L:\int21298 File Created : 4/1/2014 1:49:01 PM
Origin : Acquired, Acquisition started 9/12/2011 11:56:43 AM Acquired Date : 9/12/2011 12:36:42 PM
Project : Test By : Administrator
Method : Unit2-40minrun By : Administrator
Description : UV trace of 3H material alone
Created : 6/16/2007 8:19 AM Modified : 4/1/2014 2:05 PM
Column : Detection : UV 250nm
Mobile Phase : Temperature :
Flow Rate : Pressure :
Note :
[min.]Time
10 20 30 40
[mV]
Volta
ge
-1.0
-0.5
0.0
0.5
1.0
1.5
2.0
8.5
1
L:\ int 21298 - Unit 2 UV
4/1/2014 2:05 PM Page 1 of 2Chromatogram L:\int21298.PRM
![Page 6: MT-1732products.moravek.com/DataSheets/MT1732DS.pdf · MT-1732 Hypoxanthine, [8-3H]- Lot 212-131-009-A-20091216-DG A) All chromatograms were run using the HPLC method described on](https://reader036.pdfslide.net/reader036/viewer/2022071001/5fbe5b435552cf28e6780309/html5/thumbnails/6.jpg)
Result Table (Uncal - L:\int21298 - Unit 2 UV)
CompoundName
Reten. Time[min]
Area[mV.s]
Height[mV]
Area[%]
Height[%]
Efficiency[th.pl]
Eff/l[t.p./m]
Symmetry/Tailing[-]
Response Factor
Resolution[-]
1 8.46 23.885 1.48 100.00 100.00 7084.65 141693.02 0.85
Total 23.885 1.48 100.00 100.00
4/1/2014 2:05 PM Page 2 of 2Chromatogram L:\int21298.PRM
![Page 7: MT-1732products.moravek.com/DataSheets/MT1732DS.pdf · MT-1732 Hypoxanthine, [8-3H]- Lot 212-131-009-A-20091216-DG A) All chromatograms were run using the HPLC method described on](https://reader036.pdfslide.net/reader036/viewer/2022071001/5fbe5b435552cf28e6780309/html5/thumbnails/7.jpg)
MT-1732Hypoxanthine, [8-3H]-
Lot 212-131-009-A-20091216-DG
Chromatogram Info:
File Name : L:\int21299 File Created : 4/1/2014 1:49:01 PM
Origin : Acquired, Acquisition started 9/12/2011 1:29:33 PM Acquired Date : 9/12/2011 2:09:32 PM
Project : Test By : Administrator
Method : Unit2-40minrun By : Administrator
Description : UV trace of standard material alone
Created : 6/16/2007 8:19 AM Modified : 4/1/2014 2:07 PM
Column : Detection : UV 250nm
Mobile Phase : Temperature :
Flow Rate : Pressure :
Note :
[min.]Time
10 20 30 40
[mV]
Volta
ge
0
50
100
150
200
250
8.1
1
L:\ int 21299 - Unit 2 UV
4/1/2014 2:07 PM Page 1 of 2Chromatogram L:\int21299.PRM
![Page 8: MT-1732products.moravek.com/DataSheets/MT1732DS.pdf · MT-1732 Hypoxanthine, [8-3H]- Lot 212-131-009-A-20091216-DG A) All chromatograms were run using the HPLC method described on](https://reader036.pdfslide.net/reader036/viewer/2022071001/5fbe5b435552cf28e6780309/html5/thumbnails/8.jpg)
Result Table (Uncal - L:\int21299 - Unit 2 UV)
CompoundName
Reten. Time[min]
Area[mV.s]
Height[mV]
Area[%]
Height[%]
Efficiency[th.pl]
Eff/l[t.p./m]
Symmetry/Tailing[-]
Response Factor
Resolution[-]
1 8.13 4292.539 257.76 100.00 100.00 6023.18 120463.64 0.84
Total 4292.539 257.76 100.00 100.00
4/1/2014 2:07 PM Page 2 of 2Chromatogram L:\int21299.PRM
![Page 9: MT-1732products.moravek.com/DataSheets/MT1732DS.pdf · MT-1732 Hypoxanthine, [8-3H]- Lot 212-131-009-A-20091216-DG A) All chromatograms were run using the HPLC method described on](https://reader036.pdfslide.net/reader036/viewer/2022071001/5fbe5b435552cf28e6780309/html5/thumbnails/9.jpg)
MT-1732Hypoxanthine, [8-3H]-
Lot 212-131-009-A-20091216-DG
Chromatogram Info:
File Name : L:\int21300 File Created : 4/1/2014 1:49:01 PM
Origin : Acquired, Acquisition started 9/12/2011 2:57:53 PM Acquired Date : 9/12/2011 3:37:52 PM
Project : Test By : Administrator
Method : Unit2-40minrun By : Administrator
Description : Radiochemical trace of 3H material co-injected with standard
Created : 6/16/2007 8:19 AM Modified : 4/1/2014 2:08 PM
Column : Detection : Radiochemical
Mobile Phase : Temperature :
Flow Rate : Pressure :
Note :
[min.]Time
10 20 30 40
[mV]
Volta
ge
0
100
200
300
400
500
600
700
3.7
1
8.6
2
10.1
3
11.0
4
L:\ int 21300 - Unit 2 Radio
4/1/2014 2:09 PM Page 1 of 2Chromatogram L:\int21300.PRM
![Page 10: MT-1732products.moravek.com/DataSheets/MT1732DS.pdf · MT-1732 Hypoxanthine, [8-3H]- Lot 212-131-009-A-20091216-DG A) All chromatograms were run using the HPLC method described on](https://reader036.pdfslide.net/reader036/viewer/2022071001/5fbe5b435552cf28e6780309/html5/thumbnails/10.jpg)
Result Table (Uncal - L:\int21300 - Unit 2 Radio)
CompoundName
Reten. Time[min]
Area[mV.s]
Height[mV]
Area[%]
Height[%]
Efficiency[th.pl]
Eff/l[t.p./m]
Symmetry/Tailing[-]
Response Factor
Resolution[-]
1 3.69 250.583 13.01 1.25 1.77 1311.97 26239.42 2.11
2 8.59 19706.939 720.60 97.93 97.76 3151.77 63035.32 3.63 9.6
3 10.12 63.497 1.39 0.32 0.19 31914.89 638297.75 3.88 3.7
4 11.01 101.819 2.12 0.51 0.29 19284.74 385694.80 5.85 3.3
Total 20122.838 737.12 100.00 100.00
4/1/2014 2:09 PM Page 2 of 2Chromatogram L:\int21300.PRM
![Page 11: MT-1732products.moravek.com/DataSheets/MT1732DS.pdf · MT-1732 Hypoxanthine, [8-3H]- Lot 212-131-009-A-20091216-DG A) All chromatograms were run using the HPLC method described on](https://reader036.pdfslide.net/reader036/viewer/2022071001/5fbe5b435552cf28e6780309/html5/thumbnails/11.jpg)
MT-1732Hypoxanthine, [8-3H]-
Lot 212-131-009-A-20091216-DG
Chromatogram Info:
File Name : L:\int21300 File Created : 4/1/2014 1:49:01 PM
Origin : Acquired, Acquisition started 9/12/2011 2:57:53 PM Acquired Date : 9/12/2011 3:37:52 PM
Project : Test By : Administrator
Method : Unit2-40minrun By : Administrator
Description : UV trace of 3H material co-injected with standard
Created : 6/16/2007 8:19 AM Modified : 4/1/2014 2:09 PM
Column : Detection : UV 250nm
Mobile Phase : Temperature :
Flow Rate : Pressure :
Note :
[min.]Time
10 20 30 40
[mV]
Volta
ge
0
50
100
150
200
250 8.3
1
L:\ int 21300 - Unit 2 UV
4/1/2014 2:09 PM Page 1 of 2Chromatogram L:\int21300.PRM
![Page 12: MT-1732products.moravek.com/DataSheets/MT1732DS.pdf · MT-1732 Hypoxanthine, [8-3H]- Lot 212-131-009-A-20091216-DG A) All chromatograms were run using the HPLC method described on](https://reader036.pdfslide.net/reader036/viewer/2022071001/5fbe5b435552cf28e6780309/html5/thumbnails/12.jpg)
Result Table (Uncal - L:\int21300 - Unit 2 UV)
CompoundName
Reten. Time[min]
Area[mV.s]
Height[mV]
Area[%]
Height[%]
Efficiency[th.pl]
Eff/l[t.p./m]
Symmetry/Tailing[-]
Response Factor
Resolution[-]
1 8.33 4146.290 241.26 100.00 100.00 5835.27 116705.37 0.84
Total 4146.290 241.26 100.00 100.00
4/1/2014 2:09 PM Page 2 of 2Chromatogram L:\int21300.PRM
![Page 13: MT-1732products.moravek.com/DataSheets/MT1732DS.pdf · MT-1732 Hypoxanthine, [8-3H]- Lot 212-131-009-A-20091216-DG A) All chromatograms were run using the HPLC method described on](https://reader036.pdfslide.net/reader036/viewer/2022071001/5fbe5b435552cf28e6780309/html5/thumbnails/13.jpg)
MT-1732Hypoxanthine, [8-3H]-
Lot 212-131-009-A-20091216-DG
Chromatogram Info:
File Name : L:\int21297 File Created : 4/1/2014 1:49:01 PM
Origin : Acquired, Acquisition started 9/12/2011 10:38:16 AM Acquired Date : 9/12/2011 11:18:15 AM
Project : Test By : Administrator
Method : Unit2-40minrun By : Administrator
Description : UV trace of blank injection
Created : 6/16/2007 8:19 AM Modified : 4/1/2014 2:10 PM
Column : Detection : UV 250nm
Mobile Phase : Temperature :
Flow Rate : Pressure :
Note :
[min.]Time
10 20 30 40
[mV]
Volta
ge
-1
0
1
2
3
4
5
L:\ int 21297 - Unit 2 UV
4/1/2014 2:10 PM Page 1 of 2Chromatogram L:\int21297.PRM
![Page 14: MT-1732products.moravek.com/DataSheets/MT1732DS.pdf · MT-1732 Hypoxanthine, [8-3H]- Lot 212-131-009-A-20091216-DG A) All chromatograms were run using the HPLC method described on](https://reader036.pdfslide.net/reader036/viewer/2022071001/5fbe5b435552cf28e6780309/html5/thumbnails/14.jpg)
Result Table (Uncal - L:\int21297 - Unit 2 UV)
CompoundName
Reten. Time[min]
Area[mV.s]
Height[mV]
Area[%]
Height[%]
Efficiency[th.pl]
Eff/l[t.p./m]
Symmetry/Tailing[-]
Response Factor
Resolution[-]
No peak to report
4/1/2014 2:10 PM Page 2 of 2Chromatogram L:\int21297.PRM
![Page 15: MT-1732products.moravek.com/DataSheets/MT1732DS.pdf · MT-1732 Hypoxanthine, [8-3H]- Lot 212-131-009-A-20091216-DG A) All chromatograms were run using the HPLC method described on](https://reader036.pdfslide.net/reader036/viewer/2022071001/5fbe5b435552cf28e6780309/html5/thumbnails/15.jpg)
C:\Xcalibur\data\MT1732-20091216-DG 11/15/2010 10:48:14 AM MT1732-20091216-DG
RT: 0.00 - 2.00
0.0 0.5 1.0 1.5 2.0
Time (min)
0
5
10
15
20
25
30
35
40
45
50
55
60
65
70
75
80
85
90
95
100
Relative Abundance
1.531.51
1.90
0.27
0.42 1.36
1.16
0.47
1.141.120.51
0.69
0.771.66
1.09
NL:7.62E6
TIC MS MT1732-20091216-DG
MT1732-20091216-DG#1-344 RT: 0.00-2.00T: + c NSI Full ms [77.00-197.00]m/z= 133.32-145.83
m/z Intensity Relativem/z Intensity Relative
134.96 14666.2 1.43 136.30 7778.7 0.76 137.18 1027820.6 100.00 138.27 33784.5 3.29 139.17 446286.2 43.42 140.27 11161.2 1.09 141.16 6240.9 0.61 142.27 3421.3 0.33 143.11 6115.5 0.60 144.21 4604.5 0.45 145.19 6131.7 0.60
MT1732-20091216-DG #1-344 RT: 0.00-2.00 AV: 344 NL: 1.03E6T: + c NSI Full ms [77.00-197.00]
80 100 120 140 160 180
m/z
0
10
20
30
40
50
60
70
80
90
100
Relative Abundance
137.18
139.17
159.08149.09 183.02114.23 168.08 194.98134.96105.0192.1282.12
![Page 16: MT-1732products.moravek.com/DataSheets/MT1732DS.pdf · MT-1732 Hypoxanthine, [8-3H]- Lot 212-131-009-A-20091216-DG A) All chromatograms were run using the HPLC method described on](https://reader036.pdfslide.net/reader036/viewer/2022071001/5fbe5b435552cf28e6780309/html5/thumbnails/16.jpg)
98
76
54
32
1pp
m
8.0668.0698.1248.168
1.00
PC 1.00
GB 0
LB 0.00 Hz
SSB 0
WDW no
SF 320.1305850 MHz
SI 32768
SFO1 320.1321857 MHz
PL1 2.00 dB
P1 10.00 usec
NUC1 3H
======== CHANNEL f1 ========
TD0 1
D1 2.00000000 sec
TE 300.0 K
DE 6.00 usec
DW 81.000 usec
RG 46341
AQ 1.3271540 sec
FIDRES 0.376760 Hz
SWH 6172.839 Hz
DS 2
NS 5000
SOLVENT D2O
TD 16384
PULPROG zg
PROBHD 5 mm DUX 3H−1H
INSTRUM spect
Time 15.21
Date_ 20100210
PROCNO 1
EXPNO 1
NAME MT1732−20091216−DG
Batch 20091216−DG
MT1732 3H NMR in D2O
