APPLICATIONS

R&D BiotechnologyMolecular biology and proteomic

Genotype determination

Pharmacology and ToxicologyToxic analysis, Metabolism studies

Forensic MedicineDNA/RNA tests, Drugs and toxics detection

Food & EnvironmentGMO detection, Hygiene controls

Materials research

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DISTRIBUTOR

www.precellys.comprecellys@bertin.frPhone : +33(0)139 306 118Fax : +33(0)139 306 185

VISIT OUR PROTOCOLS DATABASE

Take a look to scientifi c publications and other application formson our website :www.precellys.com

CONTACT

BERTIN TECHNOLOGIESParc d’Activités du Pas du Lac10, bis Avenue Ampère - BP28478180 Montigny-le-BretonneuxFRANCE

SAMPLE PREPARATION & CELL LYSIS

www.precellys.com

NEW GENERATION Tissue homogenizer

NEW GENERATION TISSUE HOMOGENIZER Sample Prep and Cell Lysis

Precellys is your solution for sample preparation and cell lysis

The reliable, fast and high-throughput homogenizer to extract DNA, RNA or proteins from animal, human and plant tissues, micro-organisms, spores, bones, etc.

FAST - Homogenize up to 24 samples in few seconds

EFFICIENT - Retain integrity of any biological molecule DNA, RNA, protein, drug, etc.

FLEXIBLE - Adapt protocols and consumables from soft to hard tissues (micro-organisms, animal or vegetal tissues)

CROSS CONTAMINATION FREE - 2mL single-use tubes

REPRODUCTIBLE - Standard protocols

HIGH-QUALITY - Robust, ergonomic and programmable

Precellys®24

PRECELLYS®24 : Bead beating homogenizer

Chromatograms of liquid / liquid extraction from animal tissues.

DNA isolation from plant tissues.

Western Blot from proteins in micro-organisms.

Precellys kitReady to use 2mL tubes fi lled with beads

ANIMAL & HUMAN TISSUES

1. Load from 1 to 24 tubes 2. Patented system for quick and safe blocking

4. Bead beating motion, homogenization is achieved in few seconds

3. Set speed, time, cycle and break.

PLANT TISSUES

MICRO-ORGANISMS

Grind and homogenize a large range of biological samples, compatible with any analysis DNA-RNA Electrophoresis, Liquid Chromatography, Western Blot etc.

5. Ready for analysis

DNARNADrugsProteins

Developed to be used in L3 Laboratory

L : 290 mmW : 375 mmH : 370 mmH of opened cover : 600 mm

Weight : 29,8 kg

Universal Power :100 - 230 VAC / 50 - 60 z

VKOI & VKO5 Small Glass Beads

Special design for micro-organism lysis. Smooth impacts break the

membrane of cells,bacterias, spores, etc.

CK14 Small Ceramic Beads

Performs perfect homogenization of any soft animal, human and plant

tissues : Brain, liver, leaf, etc.

MK28 Big Metal Beads

Gives the ultimate strength to grind challenging samples of

animal, human and plant tissues : Hair, bones, corn, etc.

CK28 Big Ceramic Beads

Offers more strength for the homogenization of harder animal,

human and plant tissues :Heart, nails, nuts, etc.

6. Technical Specifi cations

Example :

Example :

Example :

� Duality: 7 mL & 2 mL tubes

� Flexibility: from 1 mg to 5 g

� Efficiency: High yield and integrity for

Extend your Precellys®24 experience

with 7 mL lysing tubes. Handle all sample preparations with the same equipment, not only from soft to hard tissues but also from 1mg to 5000 mg. Maximize your results and save time!

7 mL 2 mL

Precellys®24-Dual tissue homogenizer allows the simultaneous homogenization of six 7 mL lysing tubes or

twelve 2 mL lysing tubes.

Discover the Discover the Discover the Discover the newnewnewnew PrecellysPrecellysPrecellysPrecellys®24242424----DualDualDualDualThe only efficient large volume tissue homogenizer

Now you can choose

7 mL Precellys Lysing Kits

Dual Dual Dual Dual Actual size

DNA, RNA, proteins…

Cat No.0904-01 Cat No.0907-04 Cat No.0907-06 Cat No.0907-052.8 mm ceramic beads

Hard tissue homogenizing

CK28

Glass and ceramic beads

Soil grinding

0.1mm ceramic beads

Micro-organism lysingGlass mix beads

Micro-organism lysing

Soil Mix CK01 VK01/VK05

To optimize your grinding efficiency add beads of 5.0mm ceramic beads(03961-1-106) or 6.8mm ceramic beads(03961-1-107)

Combine yield and integrity of thermo sensible molecules with the association of Cryolys cooling option and Precellys®24-Dual.Precellys® 24 system is the only cooled equipment using bead beating in large volume.

CONTACT US

AsiaElina MACHEFERmachefer@bertin.fr

+852 9860 6075

AmericaCaroline Johnstonjohnston@bertin.fr+1 (302) 351 4429

EuropeRomain VEROLLETprecellys@bertin.fr+33 139 306 118

Cool your samples with Cryolys

Biological applications – 7 mL vials37

12-8

10-D

U02

7 / N

ovem

ber

2009

Drug extraction6000 rpm - 1x20s4 ml Buffer0904-011 gMuscle, Kidney,

Liver (Pork)

Fine powdering6000 rpm - 2x30sno buffer0904-012 tablets (0,86g)

Yeast Brewer's tablets

DNA extraction5000 rpm - 3x15s4 ml Buffer0907-04 + 2 beads

03961-1-1061 g

Microorganisms from Soil

RNA extraction6500 rpm - 2x20s5 ml Trizol0907-06500 mgLactic bacteria

from curd

Microorganism

s

Drug extraction5500 rpm - 2x10s4 ml Acetonitril70%0904-011 gWhole brain (rat)

Drug extraction6500 rpm - 2x20s*3 ml Acetonitril50%0904-01

1,2 - 1,4 gWhole lung (rat)

RNA extraction6000 rpm - 2x15s2,4 ml Buffer

0904-011 gHeart Anim

als tissues

Enzymatic activity6000 rpm - 2x60s*1 ml Buffer0904-01 + 1 bead

03961-1-1071 seedDurum wheat

DNA extraction6000 rpm - 2x30sno buffer0904-01 + 2 beads

03961-1-1071 seedCorn grain

Virus detection6500 rpm - 2x20sno buffer0904-01 + 2 beads 03961-1-106 + 2

beads 03961-1-107

2 g (250 seeds)Seeds tomato

RNA extraction5500 rpm - 2x15s1 ml Buffer0904-011 gLeaves Thyme

RNA extraction5500 rpm - 2x15s1 ml Buffer0904-011 gLeaves basilica

Plant tissues (leaves, seeds)

AnalysisPrecellys protocolsBuffer

Precellys lysingkitWeightSamples

* With Cryolys cooling option.

MicroTubes prefilled with beads0.5mL - 2mL - 7mL

Precellys®24-DualTissue Homogenizer

Precellys lysing kits

Precellys lysing kits 2010

Choose the Precellys lysing kit for your samples!

LYSE ! HOMOGENIZE ! GRIND !

VK01 VK05 VK01-05 CK01 CK14 CK28 CKMix MK28 Soil Mix

human cells ���� ���� ���� ����

gram+ ���� ���� ���� ����

gram- ���� ���� ���� ����

bacteria ���� ���� ���� ����

yeast ���� ���� ����

fungi ���� ���� ����

MICRO BIAL

spores ���� ���� ����

brain ���� ����

liver ���� ����

kidney ���� ����

ear ���� ���� ����

lung ���� ���� ����

heart ���� ���� ����

spleen ���� ���� ����

tail ���� ����

cornea ���� ����

artery ���� ����

colon ���� ����

intestine ���� ����

pancreas ���� ����

muscle ���� ���� ����

skin ���� ���� ����

spincor ����

bone ����

teeth ����

ANIMAL

hair ����

leaves ���� ���� ����

arabidopsis ���� ���� ����

seeds ���� ���� ����

corn ���� ���� ����

nuts ���� ���� ����

rice ���� ����

PLANT

wheat ���� ����

soil ����

fecal ���� SOIL sediment ����

CAT.NO. DESCRIPTION TUBES PACK

03961-1-001 Hard tissue grinding MK28 2mL STD 50 preps

03961-1-002 Hard tissue homogenizing CK28 2mL STD 50 preps

03961-1-003 Soft tissue homogenizing CK14 2mL STD 50 preps

03961-1-203 Soft tissue homogenizing CK14-0,5mL 0,5mL STD 50 preps

03961-1-004 Tough micro-organism lysing VK05 2mL STD 50 preps

03961-1-204 Tough micro-organism lysing VK05-0.5mL 0,5mL STD 50 preps

03961-1-005 Micro-organism lysing VK01 2mL STD 50 preps

03961-1-006 Soil grinding SK38 2mL STD 50 preps

03961-1-007 Hard tissue homogenizing CK28-R 2mL-R 50 preps

03961-1-008 Hard tissue grinding MK28-R 2mL-R 50 preps

03961-1-009 Tissue homogenizing CKMix 2mL STD 50 preps

0904-01 Tissue homogenizing CK28 7mL 50 preps

0907-04 Soil grinding CK14/VK01 7mL 50 preps

0907-05 Micro-organism lysing VK01/VK05 7mL 50 preps

0907-06 Micro-organism lysing CK01 7mL 50 preps

03961-1-106 5.0mm Zirconium oxide beads - Beads in bulk

03961-1-107 6.8 mm Zirconium oxide beads - Beads in bulk

LYSE !HOMOGENIZE ! GRIND !

Cat. No. 03961-1-005 (2mL tubes)

Cat. No.03961-1-204 (0,5mL tubes)Cat. No. 03961-1-004 (2mL tubes)

Application Example:- Proteins extraction from Mycobacterium Smegmatis

Cat. No. 03961-1-203 (0,5mL tubes)Cat. No. 03961-1-003 (2mL tubes)

Application Examples:- Prion extraction for ESB- RNA extraction for mouse liver

CK14

Cat. No. 03961-1-002 (2mL tubes)Cat. No. 03961-1-007 (2mL reinforced tubes) Cat. No. 0904-01 (7mL tubes)

Application Examples:- Proteins extraction from mouse heart- RNA extraction from human skin

MK28

Cat. No. 03961-1-001 (2mL tubes)Cat. No. 03961-1-008 (2mL reinforced tubes)

Application Examples:- DNA extraction from rice Grain for GMO determination- Pulverization of Hair for drugs analysis

Cat. No. 03961-1-006 (2mL tubes)Cat No. 0907-04 (7mL tubes)

Application Example: - DNA/RNA extraction from environmental samples

Cat. No. 03961-1-009 (2mL tubes)

Application Example:- DNA extraction from leaf

VK05

Big beads for 7 mLnew

Cat. No. 0907-05 (7mL tubes)

Application Example:- Recombinant proteins extraction from E.Coli

Cat. No. 0907-06 (7mL tubes)

Application Example:- RNA extraction from Lactic bacteria from curdApplication Examples:

- Virus detection from tomato seedsAdd 2 beads of 03961-1-106 and 2 beads of 03961-1-107 in the 7mL tubes 0904-01- DNA extraction from Microorganisms from soilAdd 2 beads of 03961-1-106 in the 7mL tubes of 0907-04

Cat. No. 03961-1-106 = 5.0mm Zirconium oxide beadsCat. No. 03961-1-107 = 6.8mm Zirconium oxide beads

Add beads in your 7 mL lysing tubes to optimize your grinding efficiency

VK01 - VK05new

new

1.4 mm ceramic beads

2.8 mm ceramic beads

Ceramic mix beads

2.8 mm metal beads

Glass and ceramic mix beads

0.5 mm glass beads

- VK01 = 0.1 mm glass beads

- VK01 / VK05 = glass mix beads

0.1 mm ceramic beads

CK28

CKMix

Soil Mixnew

CK01

Login and find application protocolswww.precellys.com – Application CenterFor customer suppory, email usprecellys@bertin.fr

Hard tissue grindingSoft tissue homogenizing

Tissue homogenizing

Hard tissue homogenizing Soil grinding

Micro-organism lysing

Micro-organism lysing

50 prepsReady-to-use

Quantity discount available

0.5mL 2mL

2mL 2mL-R7mL

2mL

2mL 2mL-R

2mL7mL

2mL

0.5mL 2mL

7mL

7mL

Tough micro-organism lysing