Ms. Athittaya Lakhampa

Ms. Nutthaporn Thaweesapand

Dr. Piyachat ChuysinuanDr.-Ing. Patcharakamon Nooeaid

Polymer Materials Technology, Faculty of Agricultural Product Innovation and Technology, Srinakharinwirot UniversityLaboratory of Organic Synthesis, Chulabhorn Research Institute

One-pot Fabrication of Hydroxyapatite/Biopolymer

Composite

Scaffolds for Bone Tissue Engineering

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Bone is… a composite material

Inorganic material Organic material

Hydroxyapatite Protein-based

- Collagen

Bone tissue Osteons and Haversian

canals

Fibrepatterns

Fibres Mineralized collagen

fibrils

Collagen & Hydroxyapatite

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Bone Tissue Engineering >> Composite Scaffolds

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Made of two or more distinctly different materials such as ceramics and polymers

Development of an interconnected CaP-polymer scaffold takes advantages of both

CaP and polymers

Polymer in CaP scaffolds increased toughness and compressive strength

Composite Scaffolds

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Non-toxicity

Porous structure for

supporting cell growth

Osteoconductivity

Biomaterials used

Sufficient mechanical properties

Requirements for Bone Scaffolds

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➢ Powder pressing method

➢ Electrospinning method

➢One-pot process freeze-drying method

Preparation of the Composite Scaffolds

Processability

Low cost

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Sodium Alginate

➢ A is a linear polysaccharide copolymer of (1-4) linked β-D-mannuronic acid (M) and α-L-guluronate (G) monomers

➢ It can be isolated from algae and seaweed

Gelatin

➢ Derived from collagen obtained from various animal body parts

➢ Biocompatible➢ Translucent, colorless, brittle➢ Non-cytotoxic

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To synthesize hydroxyapatite from eggshell waste and simultaneously fabricate HAP/alginate/gelatin composite-based scaffolds by using one-pot process and freeze-drying method

To investigate physical-chemical properties of the fabricated scaffolds

Aims of Work

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CaO powder from eggshell

K2HPO4 3H2O

NH4OH

Precursors for HAP synthesis

Gelatin from porcine skin

Alginate powder

Polymers

Materials

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Experimental

Trials Samples Reaction medium

1 HAP control DI water

2 HAP/Alg Sodium alginate solution

3 HAP/Gel Gelatin solution

4 HAP/Alg/Gel Sodium alginate/gelatin solution

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I. Sentering eggshell powder

CaO

II. Preparation of the HA/Biopolymer composite scaffolds

K2HPO4

CaO/Gelatin/alginatesolution

Control pH

Casting Freezing and freeze-drying

One-pot Fabrication of Hydroxyapatite/Biopolymer Composite Scaffolds

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Results & Discussion

Trial 1 : composite scaffolds received from One-pot method

5 15 25 35 45 55

Inte

nsi

ty (

a.u

.)

2 Theta (Degree)

Alg/Gel/CaO (2-6-1)

Alg/Gel/CaO (2-6-2)

Alg/Gel/HAp (2-6-1)

XRD images of the composites containing 1 and 2% HAP

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Results & Discussion

Trial 2 : composite scaffolds received from One-pot method

FTIR spectra of (a) HA sample synthesized in Alg/Gel/CaO (2-6-1),(b) Alg/Gel/CaO (2-6-2) and (c) Alg/Gel/HA (2-6-1)

5001000150020002500300035004000

%Tr

ansm

itta

nce

Wavenumber (cm-1)

(a) Alg/Gel/CaO (2-6-1)

(b) Alg/Gel/CaO (2-6-2)

(c) Alg/Gel/HAp (2-6-1)

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Results & Discussion

Trial 3 : composite scaffolds received from One-pot method

SEM micrographs of the HA samples synthesized in in (a) Alg/Gel/CaO (2-6-1),(b) Alg/Gel/CaO (2-6-2) and (c) Alg/Gel/HA (2-6-1)

(a) Alg/Gel/CaO (2-6-1) (b) Alg/Gel/CaO (2-6-2) (c) Alg/Gel/HAp (2-6-1)

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Future work

To study the compressionproperties of composite scaffolds

To study biodegradabilityproperties of composite scaffolds

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