ood OOD ABS A CRYSTAL BALL LabsLabs - AOAC Europe … · Dr. Eric Konings, President of AOAC International, Nestle, Switzerland í ò: ì - í ó: ì EU policy on contaminants in

21 - 22 MAY 2015 AT FAZER CONFERENCE CENTER

FLEMINGGATAN 18 STOCKHOLM SWEDEN

FOOD LABS IN A CRYSTAL BALL

- FUTURE CHALLENGES

IN FOOD ANALYSIS

Food Food Food

LabsLabsLabs

AOAC EUROPE - NMKL - NORDVAL INTERNATIONAL

SYMPOSIUM 2015

EUROPE SECTION OF

AOAC INTERNATIONAL

AOAC Europe is a section of AOAC INTERNATIONAL and was

established in 1989 It is an organization of professional

scientists that exchange knowledge and information to help

each other excel in their profession

The activities of the section are of interest to many

stakeholders from industry and trade consumer and

environmental protection agencies public authorities and

regulators in Europe andor Mediterranean countries

wwwaoaceuropecom

NordVal International performs a third-party review and

certifies alternative chemical and

microbiological methods for food

feed water faeces and

environmental testing

NordVal offers

a user-friendly validation

protocol

scientific confirmation policies

specified acceptance criteria

independent and rapid

approval procedures

guidance in the validation

process

a network for analytical experts

reliable analytical methods within chemical

microbiological and sensorial methods

independent reviews and NordVal

International certifications of alternative

methods

relevant guidelines

coursesworkshopsseminars

an updated list of contact persons of the

Nordic national reference laboratories

NMKL offers

wwwnmklorg

The Nordic Committee on Food

Analysis NMKL was founded in

1947 and consists of chemists

microbiologists sensory analysts and

statisticians from the five Nordic

countries Denmark Finland Iceland

Norway and Sweden

NMKL is linked to the Nordic Council

of Ministers

2

Program Thursday 21 May - Plenary Session 4

Program Friday 22 May - Chemical targets 5

Program Friday 22 May - Biotargets Microbiology 6

Program Friday 22 May - Plenary Session Floor plan 7

Presentation of the Speakers at the Plenary Session 21 May 8-12

Presentation of the Speakers at the Chemistry Session 12-18

Presentation of the Speakers at the Microbiology Session 19-23


Poster abstracts 26-38

List of participants 39-42

Hilde Skaringr Norli NMKL Secretary General Norwegian Veterinary Institute Norway

Klaus Reif Phytolab GmbH amp Co Germany

Tuija Pihlstroumlm National Food Agency Sweden

Arne Hoslashjgaringrd Jensen Danish Veterinary and Food Administration Denmark

Eric Verdon ANSES - French Agency for Food Environmental and Occupational Health amp Safety France

Pierre Metra Merieux NutriSciences France

Dag Groslashnningen Norwegian Veterinary Institute Norway

Sune Eriksson Chiron Norway

Suvi Ojanpera MetropoliLab OY Finland

Franklin Georgsson Mattis Iceland

Bert Poumlpping Merieux NutriSciences Corporation

3M Food Safety

Agilent Technologies

BergmanLabora AB

Biolab AS

FAPAS

Food Diagnostics

Foss Analytical AS

Labolytic AS

Larodan AB

Phenomenex Aps

Randox Food Diagnostics

R-Biopharm AG

SCIEX

Thermo Fisher Scientific

Exhibitors

Organisation committee

Contents

3

PROGRAM 21 MAY PLENARY SESSION

1230 - 1300 Registration Exhibition

Moderators Dr Ulla Edberg Chair of NMKL National Food Agency Sweden

Dr Klaus Reif President of AOAC Europe PhytoLab GmbH amp Co KG

Germany

1300 - 1315 Opening Welcome Speech

Director General Stig Orustfjord National Food Agency Sweden

1315 - 1330 Welcome

Chair of NMKL Dr Ulla Edberg

Chair of AOAC Europe Dr Klaus Reif

1330 - 1400 The future of food testing - which way to go

Dr Franz Ulberth European Commission Joint Research Center Belgium

1400 - 1430 Future Challenges in Food Analysis

Prof Dr Med Jan Alexander Norwegian Scientific Committee of Food

Safety Norway

1430 - 1500 Lean Lab - Speed Productivity and Quality

Dr Bernd Renger Bernd Renger Consulting Germany

1500 - 1545 CoffeeTea break and Exhibition

1545 - 1600 Standard methods versus method criteria

Mrs Hilde Skaringr Norli NMKL Norwegian Veterinary Institute

1600 - 1630 Industry and an SDOrsquos perspective

Dr Eric Konings President of AOAC International Nestle Switzerland

1630 - 1700 EU policy on contaminants in food and feed an indispensable need for relia-

ble quick and inexpensive testing for an effective enforcement approach

Mr Frans Verstraete European Commission Directorate General for Health

and Consumers Belgium

1700 - 1705 Closure

1900 Dinner at Fazer

4

22 May Session for Chemical targets

Moderators Suvi Ojanpera MetropoliLab OY Finland


0900 - 0930 Food Control authentication using contaminant profile

Dr Stig Valdersnes National Institute of Nutrition and Seafood Research Norway

0930 - 1000 Strategies for analysis of unknown samples Non targeted screening with LC-TOF

Dr Johan Roseacuten National Food Agency Sweden

1000 - 1030 An overview of new technologies in veterinary chemical residue control in food by

rapid methods immuno-microbio-receptor-biosensing

Dr Valerie Gaudin Anses - Laboratory of Fougeres France

1030 - 1100 Coffeetea break Exhibition Posters

1100 - 1130 Preparedness In situ trace element analysis of fish scales

Dr Belinda Flem Geological Survey of Norway

1130 - 1200 Microplastic in Food and Environment

Dr Ruud J B Peters Wageningen University the Netherlands

1200 - 1230 New methods for allergens

Dr Bert Popping Meriuex NutriScience Corporation

1230 - 1330 Lunch Exhibition Posters

1330 - 1400 Plant toxin amp Food Adulteration

Dr Joerg Stroka European Commission - Joint Research Centre Belgium

1400 - 1430

Simple is to be great ndash SweEt

Swedish Ethyl Acetate multi residue method for analysis of pesticide residues

Dr Tuija Pihlstroumlm National Food Agency Sweden

1430 - 1500 ContaminantsPerfluorinated Alkyl Substances

Dr Xenia Trier Technical University of Denmark

1500 - 1530 Coffeetea break Exhibition Poster

1530 - 1700 PLENARY SESSION See page 7

5

22 May Session for Biotargets Microbiology

Moderator Dr Gro S Johannessen Norwegian Veterinary Institute

Dr Hans Lindmark National Food Agency Sweden

0900 - 0930 Sampling and analytical methods at early process steps to ensure the food safety

of final products

Dr Taran Skjerdal Norwegian Veterinary Institute Norway

0930 - 1000 Listeria-outbreak in Denmark self-monitoring food control sampling

Dr Jens Kirk Andersen Technical University of Denmark

1000 - 1030 GMI Global Microbial IdentifiermdashThe future of microbiology

Prof Joslashrgen Schlundt DTU Management Engineering Denmark


1100 - 1130 Microbiological examinations by using MALDI-TOF

Dr Annica Tevell Aringberg National Veterinary Institute Sweden

1130 - 1200 Microbiota and the significance for human health

Prof Tor Lea Norwegian University of Life Sciences NMBU Norway

1200 - 1230 Verification of microbiological methods

Dr Charlotta Engdahl Axelsson Eurofins Sweden


1330 - 1400 Validation of alternative methods

Dr Gail Betts Campden BRI United Kingdom

1400 - 1430

Harmonization of the NordVal International validation protocol with the new ISO

161402015 protocol for the validation of alternative microbiological methods

Dr Sven Qvist NordVal International Denmark

1430 - 1500 Standardised molecular detection of waterborne pathogens

Dr Jakob Ottoson National Food Agency Sweden


1530 - 1700 PLENARY SESSION See page 7 6

Plenary 22 May


Dr Klaus Reif President of AOAC Europe PhytoLab GmbH amp Co KG Germany

1530 - 1600 SNIFFER Sensory devices network for food supply chain security New fluorescent

probes for CBR agents

Prof Tomas Torroba University of Burgos Spain

1600 - 1630 Foodomics 21st Century Food Science Using Omics Tools

Prof Dr Alejandro Cifuentes Laboratory of Foodomics CIAL National Research

Council of Spain

1630 - 1645 Poster Award and Closure

Auditorium

Houmlrsal Braumldstapeln

Entre

Toilets

Wardrobe

Exhibition area

Conference location

7

Dr Ulla Edberg Chairman of NMKL National Food Agency Sweden

E-mail UllaEdbergslvse

Chairman of NMKL and the Swedish National Committee and Ass Professor at the

University of Uppsala UE has for long worked in the field of quality assurance

method development and analysis of food in the National Food Agency The agency

has the task of protecting the interests of the consumers by working for healthy

dietary habits safe foods and fair practices in the food trade The tools are

regulations recommendations and communication UE has for many years been the

Swedish representative in CENTC 275 Food Analysis-Horizontal Methods and in

Codex Committee om Methods of Analysis and Sampling

Welcome from NMKL

NMKL Nordic Committee on Food Analysis is a network for chemists microbiologists and sensory analysts

working in food laboratories (private and governmental) food industry research institutions and in food

control authorities NMKL was established in 1947 The members of NMKL are appointed expert from the five

Nordic countries Denmark Finland Iceland Norway and Sweden

NMKL cooperates with several international organisations and has interested parties from more than 40

countries worldwide NMKL elaborates and collaboratively validate analytical methods elaborates guidelines

on quality assurance (a list is given at page 41) and arranges courses and workshops

NMKL also deals with rapid methods and holds the secretariat of NordVal International NordVal International

gives an independent review of alternative methods It is with great pleasure that we can welcome you to this

joint AOAC Europe - NMKL - NordVal International Symposium

Mr Stig Orustfjord Director General National Food Agency Sweden E-mail StigOrustfjordslvse

Mr Orustfjord has been the Director General of NFA since September 2013

Prior to his current position he served as Director General and Deputy General

Director at the Swedish Social Insurance Agency Between 2008 and 2010 he was

the provincial Director General at the County Administrative Board of Stockholm Stig

Orustfjord has previously worked as Director at the Social Insurance and National

Insurance Administration and has been the Head of the care of the elderly and

disabled in the city of Stockholm

Stig Orustfjord has conducted two studies commissioned by the government In 2010

he investigated on behalf of the of Education Board if the repayment of student debts

could be improved In 2013 he investigated the national preschool warranty He has

also served as Chairman of the Swedish Director Association an association for

managers under the Academy Association SSR

8

Dr Klaus Reif Chair of AOAC Europe PhytoLab GmbH Vestenbergsgreuth

Germany E-mail klausreifphytolabde

Dr Reif holds an PhD in Natural Science from the Friedrich-Alexander-University

Erlangen Institute for physical chemistry and in the Research Center of Siemens AG

Erlangen He is responsible for method development and method validation for the

registration procedure of phytopharmaceuticals dietary supplements and food residue

analysis routine analysis of food and cosmetics product release and stability tests with

HPLC of herbal raw materials and finished herbal products specialist on the

development of analytical methods based on HPLC Nuremberg for the analysis of food

and botanical products He is an regular invited speaker and poster presenter in a

various international scientific symposia He is an active member of AOAC International

(Pool of Experts member of different expert review panels general reviewer for the

Journal of AOAC International) President of the Executive Committee of AOAC Europe

Section Member of the Scientific Advisory Board of the American Herbal

Pharmacopoeia (AHP) Associated Member of the American Herbal Products Association

AHPA (Member of the Analytical Laboratories Botanical Raw Materials and Standards

Committee)

Welcome from AOAC Europe

AOAC Europe includes all members of the EU (except Netherlands) and all countries around the Mediterranean Sea

The section shall promote and support the purpose and objectives of AOAC International promoting quality

measurements and methods validation in the analytical Sciences as stated as

promoting interest and participation in the Associations purpose ad programs

providing a regional focus and forum for the Association and its members and for addressing regional analytical

needs

providing a means of increasing the knowledge and technical skills of analytical scientists especially through

seminars forums workshops and other similar technical updates

providing means to improve communications with the Associations membership

identifying and communicating with appropiate non-member laboratories organizations educational

institutions firms and individuals in the region in order to encourage their participation other organizations in

Section and Association programs

developing Cooperative relationships with educational institutions government industry and other

organizations with an interest in method development and validation

Dr Franz Ulberth European Commission Joint Research Centre

Belgium E-mail FranzULBERTHeceuropaeu

Franz Ulberth is Head of the Standards for Food Bioscience Unit at the European

Commissions Joint Research Centre ndash Institute for Reference Materials and

Measurements (JRC-IRMM) Franz graduated (PhD) in Food Science and

Biotechnology from the University of Natural Resources and Applied Life

Sciences (BOKU) in Vienna Austria In 1994 he was appointed professor of food

chemistry at the same university Franz joined JRC-IRMM in 2002 as a

programme co-ordinator for food and environmental reference materials at the IRMM In 2007 Franz was nominated

Head of the Standards for Food Bioscience Unit at the JRC-IRMM He represents the Joint Research Centre in relevant

food related technical committees of standards developing organisations such as the European Committee for

Standardization International Organization for Standardization AOAC International and the Codex Alimentarius Franz

served for a long time on the editorial board of Food Chemistry European Journal of Lipid Science and Technology and

currently is editorial board member of Food Additives and Contaminants (continued on page 10)

9

The future of food testing ‐ which way to go

The free movement of safe and wholesome food is an essential aspect of the internal market and contributes

significantly to the health and well-being of EU citizens and to their social and economic interests Due to globalisation

and the availability of new technological processes the European food and feed sector is becoming more and more

complex Thousands of chemical measurements are carried out every day across Europe to lay the foundation of a

decision making process mostly to decide whether an item conforms to specification or not When deciding whether

a tested item conforms to certain specifications the uncertainty associated with the test result has to be taken into

account Because of the wide-ranging consequences of such decisions analytical data have to be comparable and

reliable Mutual recognition of measurement data is extremely important to avoid duplication of analyses thereby

reducing costs and resources associated with testing Evidence based decision making is only possible if the underlying

data are reasonably accurate Analysts are eager to select an analytical system that fulfils to the greatest extent

possible this requirement however constraints such as availability of resources might set certain limits to this

endeavour Whatever the mechanism is for choosing a method for official food control evidence has to be provided

that the method delivers valid results and that the performance of the method is fit-for-purpose Collaboratively

trialled and if possible standardised methods are seen by many as the gold standard for official control and dispute

resolution EU legislation requires using such methods if they exist and Codex Alimentarius endorsed methods need

also to be ring-trialled However the organisation of collaborative studies is a resource intensive process and

therefore alternatives have been explored (eg the AOAC Alternative Pathway) The presentation will reflect on

strengths and weaknesses of alternative approaches for method validation and will make recommendations for future

activities to ensure data quality and validity of results

Prof Dr Med Jan Alexander Deputy director-general Norwegian Institute of

Public Health Professor in food toxicology Norwegian University of Life

Sciences E-mail JanAlexanderfhino

Jan Alexander has a background in occupational medicine has worked in the field of

environmental medicine food safety and nutrition for more than 30 years He is currently

chair of the Norwegian Scientific Committee for Food Safety and the vice chair of EFSA

Scientific Committee and previously in the Panel on Contaminants in the Food Chain and

EU Scientific Committee on Food His main research interests are in toxicology and risk

assessment food processing contaminants and intestinal carcinogenesis metals and

persistent environmental contaminants

Future Challenges in Food Analysis Access to safe and nutritious food is basic requirement for human health and the risks of unsafe food are substantial

Food analysis is an essential part of ensuring food safety and nutritious foods The risks are related to harmful parasites

bacteria viruses prions allergens chemical compounds and radioactive substances which may cause numerous health

problems ranging from infectious to non-communicable diseases such as cancer and impaired development In addition

to chemical contamination from the environment a large number of chemicals such as plant protection products food

additives food flavourings food packaging materials are used in food production Moreover a range of natural toxins

produced by fungi and algae may contaminate food and inherent natural toxicants may also occur in food plants New

technology in food production using gene modified food plants are in widespread use In a globalized world with

extensive trade with food and food ingredients consumers demand for wide variety of foods and risk of fraudulent

behaviour food safety problems may travel over long distances from one part of the world to another Hence food safety

is a challenge both at an international and a national level In 2015 the World Health Day was devoted to food safety

Methods both in microbiological and chemical analyses of food have undergone an extensive development and range

from methods using culturing to direct genetic techniques in microbiology and in chemical analysis methods using new

sophisticated instrumental techniques in spectroscopy separation and hyphenated techniques are available The link

from food to human health risk is related the extent of exposure to hazardous microbiological agents and chemicals In

this area there are new opportunities in the field of human biomonitoring using human biomaterial such as blood urine

hair and biomarkers of exposure

10


Dr Bernd Renger has more than 35 years industry experience in different managing

positions in API Manufacturing and Pharmaceutical Industry He started his professional

career with Hoechst AG as a Research and Development Chemist Since than he has held

positions as Director of Quality Control andor Quality Assurance at Mundipharma

(Limburg) Byk GuldenAltana Pharma (Singen) Baxter BioScience (Vienna) and Vetter

Pharma Fertigung (Ravensburg) He holds a degree in Organic Chemistry from the

University in Giessen Germany and is an appointed Qualified Person according to the

European regulations and has acted as a Qualified Person in the EU for more than 27

years He is an expert in Quality Systems and Quality Risk Management System design

development and implementation He is author or co-author of more than 80

publications and is a frequently invited speaker by organizations

Lean Lab ndash Speed Productivity and Quality Although laboratory operations are not the same as manufacturing processes aspects of the current management

strategies proposed to reduce cost while improving quality and efficiency ndash usually subsumed under the term ldquolean

thinkingrdquo ndash can also be applied to all laboratory activities However the usually proposed ldquoone size fits allrdquo approach

might not work In addition very often the optimistically predicted and expected savings potential is hard to achieve

leading to abandoning projects in frustration To avoid pitfalls a careful adaptation of the lean techniques must go

hand in hand with a thorough understanding of laboratory processes and the required quality standards As a

consequence any lean project must fully integrate laboratory staff Even then we must be aware that lean projects

may not lead to overwhelming economic benefits and savings in budget and staffing One benefit that can be

reasonably expected however are enhanced reliability and consistency of laboratory operations and thus results

delivered by the laboratory The tools used in lean projects may range from simple 5 S techniques used to better

organize lab working areas value stream mapping for creating better material and information flow to complex Six

Sigma projects using specific statistical evaluations or even implementing more automatic analytical systems Some

examples from the experience of the speaker will be presented

11

Mrs Hilde Skaringr Norli NMKL Secretary General Norwegian Veterinary

Institute E-mail nmklvetinstno Since 1997 Hilde Skaringr Norli has served as the Secretary General of the Nordic Committee

on Food Analysis NMKL The office of the NMKL Secretary General is hosted by the

Norwegian National Veterinary Institute where Norli has been employed since 1995

Hilde Skaringr Norli holds a CandScient degree in analytic organic chemistry from the

University in Oslo Norway and has been employed at a couple of private laboratories

and at the Norwegian Food Safety Authority Norli serves as the secretary of NordVal

International is active in AOAC International and in other international organisations

including Codex Committee on Methods of Analysis and Sampling

Standard methods versus method criteria Food control laboratories are required to be accredited to participate in proficiency testing schemes and to use fully

validated methods whenever such methods are available (EC 8822004) In some areas of food analysis there are

many available methods of analysis and luckily the development for more rapid methods and new techniques

continues It has been criticised that prescribed analytical methods are specified in the legislation (EU and Codex) The

criticism made against prescribed methods were such as

the analyst is denied freedom of choice in methodology

the procedure might inhibit the use of advanced andor new more rapid techniques

it is administratively difficult to change a method found to be unsatisfactory or inferior to another currently available

method

Therefore specific performance criteria have been elaborated for specific chemical contaminants in EU legislation and

for rational chemical methods in Codex Alimentarius Within microbiology alternative methods to the prescribed

analytical methods can be used if providing equivalent results

Industry perspective

With more than 400 factories in 150 countries and 26

specialized analytical centers millions of analytical data per

year are generated Most of these analyses are performed

at factory level using frequently alternative analytical

methods A described by ISO an alternative physico-

chemical method is an indirect method replacing an

established reference method against which it is

calibrated validated and monitored The use of alter-

native methods offers the factories many operational

advan-tages Examples of technologies currently routinely

used will be given There are many guidelines available

from international organizations as for example ISO

NordVal Eurochem IDF giving detailed information on

(individual or parts of) alternative method management

However a general harmonized guideline for the

calibration validation and monitoring of all types of

alternative methods is missing With an internationally

recognized harmonised guideline authorities may accept

the use of alternative methods rather than the reference

method for product release This will save costs and time

Dr Erik JM Konings President of AOAC INTERNATIONAL

Nestleacute Research Center Lausanne Switzerland

E-mail erikkoningsrdlsnestlecom Erik Konings studied higher professional laboratory education with majors in analytical and

clinical chemistry After graduating in 1984 he started his professional career at the then called

Food Inspection Service in Maastricht the Netherlands In 2001 he completed his PhD study

ldquoDietary folates in human nutritionrdquo at Maastricht University During this study he obtained an

MSc-degree in epidemiology He is (co)author of more than 30 scientific publications In

September 2008 he started at the European Food Safety Authority (EFSA) in Parma Italy for a

secondment as Scientific Officer at the Data Collection and Exposure Unit and from there

accepted in June 2009 a position at the Nestleacute Research Center in Lausanne Switzerland

currently in a role as Food Safety amp Quality expert He is active in several Standard

Development Organisations as AOAC INTERNATIONAL ISO and IDF and participates in the

Codex Committee on Methods of Analysis and Sampling (CCMAS)

Industry and an SDOrsquos perspective

SDOrsquos perspective

With globalization the need for harmonized standards is

a requirement to meet the demands of international

food trade ensuring safety quality and fair trade

Harmonised standards are needed in the area of

validation protocols as mentioned before but also for

methods used for (official) control purposes To achieve

this a good cooperation between regulatory bodies

standard development organisations industry

referencecommercial laboratories is needed Analytical

science is critical to ensure that products contain what

is claimed on the label or required by regulations The

AOAC INTERNATIONAL Stakeholder Panel on Infant

Formulas and Adult Nutritionals (SPIFAN) is a good

example how all stakeholders as mentioned above

come together to establish standards for global

consensus reference methods on nutrient testing in

infant formulae and adult nutritionals Endorsement of

these reference methods by the Codex Alimentarius

Commission would allow them to be promoted for use

in global trade


and Consumers E-mail FransVerstraeteeceuropaeu

Frans Verstraete graduated in 1985 as agricultural engineer at the University of Ghent

(Belgium) After his studies he held positions at the University of Ghent and thereafter at

the Belgian Ministry of Agriculture and he was for a period technical adviser of the Belgian

Minister of Agriculture He is working for the European Commission since 1997 In the Eu-

ropean Commission he had various functions but since 2000 he is working at the Direc-

torate General Health and Food Safety He is responsible for the elaboration development

and management of the EU-legislation concerning contaminants in feed and food

(continued on page 13)

12

Dr Stig Valdersnes National Institute of Nutrition and Seafood Research

Norway E-mail StigValdersnesnifesno

Stig Valdersnes is a researcher at the National Institute of Nutrition and Seafood Research

(NIFES) in Bergen Norway His research interests are focused on developing and validating

methods for the determination of chemical compounds in food and feed for research and

control purposes The methods encompasses both persistent organic contaminants such as

PFAS and BFRs metals and their species such as methylmercury and tributyltin as well as

compounds with nutritional value and additives in feed The methods exploits the wide

array of instrumental techniques available at NIFES with different chromatographic

techniques ionization techniques and detectors used for the determinations He is a

member of the Nordic committee on food analysis (NMKL) and the European

Standardization Committee (CEN) WG 10 ndash elements and their chemical species Since

2013 he has been part of the Norwegian delegation to CCMAS (Codex Committee on

Methods of Analysis and Sampling)

EU policy on contaminants in food and feed an indispensable need for reliable quick and inexpensive testing for an effective enforcement approach

The EU legislation on contaminants in food fulfils two essential objectives the protection of public health and removal of internal barriers to trade within the EU Council Regulation (EEC) No 31593 of 8 February 1993 laying down community procedures for contaminants in food is the framework for the Community action on contaminants Based on this framework Regulation maximum levels for the following specific contaminants have been established by Commission Regulation (EC) No 18812006 of 19 December 2006 setting maximum levels for certain contaminants in foodstuffs

Maximum levels have been established in feed and food for a wide range of contaminants But legislation is only effective in protecting public and animal health if the enforcement is effective and if legislation is uniformly applied across the EU The establishment of uniform sampling and analysis procedures in that respect is of major importance Regulation (EC) 8822004 of the European Parliament and of the Council of 29 April 2004 on official controls performed to ensure the verification of compliance with feed and food law animal health and animal welfare rules provides the regulatory framework for sampling and analytical procedures

EU-RLNRL networks have been established for several contaminants and are of major importance for the effective application of feed and food safety legislation The need for co-operation support and assistance for in many cases complex analysis is self evident As regards contaminants in feed only few methods of analysis have been established at EU level While for feed at EU level the approach of establishing specific methods of analysis has been followed in the field of contaminants in food the approach of establishing performance criteria has been followed

13

Food Control Authentication using contaminant profile Secure access to safe food is of fundamental importance to all people around the globe In recent years there has

been increasing focus on the adulteration of foods which may pose health risks The adulteration of food include both

food fraud by food producers and food fight due to the deliberate tampering with foods by terrorists Being able to

trace the food and feed from farmfjord to fork is therefore important for the protection of consumersrsquo health

Developing analytical techniques to verify the identity and origin of foods encompasses a wide array of techniques

from chemical noses to stable isotope analysis PCRDNA is one of the methods routinely used to determine the

authenticity of food but this method is not applicable to food products without DNA such as marine oils Marine oils

contain health beneficial nutrients such as omega-3 fatty acids and vitamin D but unrefined marine oils may also

contain elevated levels of fat soluble contaminants such as dioxins PCBs and pesticides Since there are maximum

limits for contaminants in marine oils used in food and feed these are routinely determined at NIFES For Norway it is

also important to be able to determine the authenticity of the oils since Norway is the largest importer of marine oils

from other countries and a major producer of refined marine oils The levels and distributions of contaminants are

known to depend on eg specie age season and geographical origin We therefore carried out an evaluation of

whether or not the levels of contaminants determined in authentic unrefined marine oils of different marine species

could be used to distinguish between the oil types The evaluation showed that different oil types displayed groupings

in principal component analysis The potential uses and limitations of contaminant profile for authentication purposes

will be discussed


E-mail johanrosenslvse Chemist at the National Food Agency in Uppsala Sweden JR has for long worked in the field

of food contaminants developing methods for analysis of residues of eg veterinary drugs

and pesticides JR paid interest to new techniques or workflows when they had the

potential to increase the efficiency broaden the scope or increase the accuracy of

analytical methods Hence he has been working with eg automation multiresidue

methods using LC-MSMS and also to develop methods for EU standardization One of his

current projects is to investigate how the high resolution MS technique LC-TOF can be used

for screening as well as investigations of contaminated food

Strategies for analysis of unknown samples Non-targeted screening with LC‐TOF Monitoring of chemical contaminants in food is carried out at the National Food Agency Uppsala Sweden Less than

1000 compounds are covered by the present official control programs which mainly are based on quadrupole MS

(Mass Spectrometry) A food crisis caused by accident fraud or even deliberate poisoning could though in theory

involve any compound and there are more than 20 000 000 compounds registered in internet databases LC-TOF-MS

(Liquid Chromatography Time of Flight MS) has recently been set up at the laboratory for investigations of food items

suspected to be contaminated by unknown chemical ndash ldquothe unknown samplerdquo The technique is also used to screen

for (unexpected) pesticides and could possibly replace the quadrupole MS technique for the official control programs

Another interesting future application would be to detect new or unexpected contaminants in food via what we call

ldquolearning systemsrdquo The possibility to use the TOF technique for the mentioned purposes will depend on the

application as well as future hardware and software development This presentation will give a brief discussion of

possibilities and limitation of TOF-MS for screening of food contaminants Some practical applications will also be

presented including

Screening of pesticides with access to standards andor retention times

Screening without standards or retention times for suspected contaminants The approach was used to reveal

illegal use of red color for selling fillet of pork as fillet of beef

Fully non-targeted methods for comparison of contaminated and non-contaminated samples Spiked orange

juice was used to investigate method performance

Retrospective analysis after a Cola alarm in media ldquoNewrdquo contaminant found in old data file The possibility for

DiBBs Digital BioBanks

Mrs Valeacuterie Gaudin Senior Biochemist ANSES Laboratory of Fougeres

France E-mail valeriegaudinansesfr

Valerie graduated with a Masters Degree (MSc) in Veterinary pharmacy and biochemistry

from the University of Limoges (France) and has 18 yearsrsquo experience at ANSES

the French Agency for Food Safety and Environmental and Occupational Health Safety

She is a Senior Analytical biochemist in the EURL at Anses Fougegraveres France Valeacuterie is

responsible for managing a number of research projects in the areas of antibiotic residues

veterinary medicines and emerging biosensor techniques Valerie has published more than

23 peer reviewed papers based on microbiological methods ELISA kits and biosensors

Valeacuterie participated in the publication in 2010 of the European Guideline for the Validation

of Screening Methods with the support of the European Commission (DG-SANCO) She is co

-leader of a working group drafting the ISO IDF Standard for the Validation of screening

methods for residues of veterinary drugs in milk She is also expert for the International

Honey Commission


14

Dr Ing Belinda Flem Team leader geochemistry group Geological Survey of

Norway E-mail BelindaFlemNGUNO For 15 years Flem has worked within analytical chemistry with focus on in situ analysis for at

the laboratory section at the Geological Survey of Norway (NGU-Lab) located in Trondheim

She is the team leader (section leader) of the geochemistry group at NGU whose main focus is

on urban geochemistry and mineral prospecting She is also strongly involved in a project

FarmSalmTrack at the Norwegian Veterinary Institute establishing a laser ablation

inductively coupled plasma mass spectroscopy lab and develop together with the fish farm

industry in Norway a method for tracking escaped salmon to its origin Belinda Flem was

graduated as Dr Ing Physical chemistry from the Norwegian university of science and

technology in 1996 SivIng Physical chemistry from NTH in 1991 and Engineer in Analytical

chemistry from Bergen engineering college in 1988 She has worded at Geological Survey of

Norway Since 2011 She has also worked as laboratory manager at National Food Agency at

Fosen Norway and as research assistant during her PhD graduation

Preparedness In situ trace element analysis of fish scales

Atlantic salmon as species is separated into a number of local populations in different rivers along the coastline from

north to south of Norway Homing is the most characteristic feature of Atlantic salmon (Salmo salar L) Increased

exploitation power plants diseases and introgression with escaped farmed fish pose a threat to the wild Norwegian

salmon Both The ministry of Trade Industry and Fisheries and The ministry of Climate and Environment has decreed

the fish farm industry in Norway to establish a system that can track escaped salmon back to its owner During the last

decade several methods for identifying escaped salmon and track it back to its fish farm has been investigated eg

DNA-markers fatty acid profiles trace elements stable isotopes and micro chip nose tagging In 2014 the majority of

the fish farm industry the Norwegian Veterinary Institute and the Geological Survey of Norway established an

agreement on co-operation to develop the trace element fingerprint approach in fish scales to a full scale tracking

method The growth pattern of the mineralized layer on the fish scale has radical increments (sclerites) that can be

compared to tree rings While a tree forms a new ring on a yearly basis a new sclerite is laid down in the fish scale

every 7-10 days The trace element content in these mineralized growth bands of the scale reflects those present in

the ambient water at the time of creation Trace element concentrations are analyzed by laser ablation inductively

coupled plasma mass spectroscopy (LA-ICP-MS) This is an in situ technique which makes it possible to analyze on

selected sclerites which in turn provide information from a selected time frame of the salmons life

15

An overview of new technologies in veterinary chemical residue control in food by rapid methods

from classical to innovative technologies

The screening methods are the first critical step for the control of veterinary drugs in food before confirmatory

methods Screening methods should be sensitive specific or with a wide spectrum detection depending on the target

analytes cheap quick and with high throughput of samples What are the techniques available to quickly screen for

veterinary drugs in food of animal origins Classical techniques are microbiological and immunological methods (ie

ELISA radioimmunoassays) Microbiological methods are largely used all over the world because they show the

advantages of being cost-effective and with a large spectrum of detection Immunological classical tests are usually

more specific and sensitive The trends in screening development are now focused on biosensor techniques A

biosensor is the combination of a bioreceptor (eg antibody molecularly imprinted polymer etc) and a transducer

which transforms the biological interaction in an electrical signal (eg optical electrochemical etc) The usage of

biosensors for biomedical applications (eg glucose detection in blood) started in the 1980rsquos The application of

biosensor techniques for the screening of veterinary drugs in food of animal origin is more recent but it is in continuous

development The basic principles the advantages and the drawbacks of these innovative biosensors will be discussed

here Due to their variety and their high potential biosensors are probably the future of the rapid control of veterinary

drugs in foods

Dr Bert Poumlpping Chief Scientific Officerof Corporate Food Chemistry and

Molecular Biology Meacuterieux NutriSciences Corporation

E-mail bertpoppingmxnscom

Dr Bert Poumlpping is a world-renowned scientist with a broad and international background

He studied natural sciences in Germany and UK He has held various positions of

responsibility in Research amp Development Management and Marketing for most of his

professional career in leading companies and government laboratories in the field of food

testing He is the author of over 40 peer-reviewed scientific publications in the fields of

global food safety allergen testing authenticity and genetically modified organisms

(GMO) analysis Dr Poumlpping served and serves on numerous national and international

committees as chair or member including the Board of Directors of the AOAC Research

Institute Board of Directors of the German Association of Wholesale Traders in Oils Fats

and Oil Raw Materials (GROFOR) the Executive Board of MoniQArsquos Global Food Safety

Network the European Standardization Committee (CEN) the British Standards Institute

(BSI) the German Ministry Methods Working Groups the AOAC and the German

Standardization Institute In his new position at Meacuterieux NutriSciences Poumlpping will be

responsible for leading the development and implementation of Meacuterieux NutriSciencesrsquo

innovation chemistry and molecular biology strategy

Presentation New methods for allergens

Dr Ruud JB Peters Senior scientist and Group leader Contaminants at

RIKILT Wageningen UR The Netherlands E-mail ruudjpeterswurnl Ruud Peters (1960) holds a PhD in Chemistry and has over 25 years of experience in

analytical chemistry He started out the National Institute of Public Health and the

Environment (RIVM) worked for 17 years at TNO (the Dutch Organisation for Applied

Scientific Research) and since 2007 for RIKILT the Dutch institute of food safety part of

Wageningen UR Currently he is coordinating the section Contaminants (25 researchers

and analysts) that deals with organic contaminants like dioxins and PAH process

contaminants like acrylamide melamine and nitrosamines heavy metals nanomaterials

and radioactivity He is also project leader of the National Plan Residues in food from

animal origin and of the 247 crisis organisation From a scientific point of view he is

involved in the development and application of new methods for contaminants and

residues in food and feed the identification of new and ldquounknownrdquo contaminants and

especially the last few years the development of methods for nanomaterials in food and

non-food

Micro-plastics in food and environment Detection occurrence and potential health impact Ruud JB Peters Hans Bouwmeester Peter CH Hollman

High concentrations of plastic debris have been observed in the oceans and several consumer products nowadays

contain micro-sized plastics Recent concerns are focussed on these micro plastics especially since detailed studies of

the size distribution of the plastic debris showed a gap in particle sizes below 1 millimetre It has been argued that this

could be due to continued fragmenting of the plastic particles into nano-sized particles At that point the currently

used analytical techniques introduce a great bias in the knowledge since they are only able to detect plastic particles

well above the nano-range Analytical techniques which have been developed for the detection and quantification of

engineered nanoparticles will be discussed for their potential to determine micro- and nano-sized plastics The

detection and occurrence of environmentally released micro- and nano-plastics in the human food production chain

will be reviewed and their potential health impact will be discussed

16

Dr Tuija Pihlstroumlm Swedish National Food Agency

E-mail tuijapihlstromslvse

PhD in analytical chemistry at Uppsala University

Head of pesticide unit at the National Food Agency undertaking the method development and

analysis of pesticide residues in food A continuous work with improvement of the SweEt

method Member of the Advisory Board for organizing EU RL Proficiency Tests and coordinator

of the SANCO Quality Control guidelines

Actively working in CEN NMKL (Nordic Committee on Food Analysis) and Codex


Swedish Ethyl Acetate multi residue method for analysis of pesticide residues The National Food Agency has been using a multi residue method based on extraction with ethyl acetate and the

determination by means of GC-MSMS and LC-MSMS since 1989 for the monitoring of pesticide residues in fruit and

vegetables The method has been revised continuously resulting in an improved and simplified methodology

Introduction of products of animal origin since 2009 has further enlarged the scope of the method Method benefits

include the very unique selectivity of ethyl acetate which is the basis to use it as an almost universal extraction solvent

in pesticide analysis coupled to none or only limited clean-up after extraction prior to analysis Furthermore ethyl

acetate as solvent makes it applicable both LC and GC analysis without solvent change The direct injection of ethyl

acetate to LC-MSMS has shown to separate all analytes selectivelyThe method has been validated for 450 analytes in

different crops fulfilling the criteria established in a guidance document (SANCO 125712013) Moreover the method

has shown to give acceptable results in proficiency tests organized by EU Reference Laboratories In a search of

alternative multi residue method for routine monitoring purposes the SweEt method has shown to be a reliable and

straightforward alternative to analyze pesticide residues in all kind of food samples

17

Dr Joerg Stroka Operating manager - European Union Reference Laboratory (EURL)

for Mycotoxins Institute for Reference Materials and Measurements (IRMM) in

Geel Belgium E-mai JoergSTROKAeceuropaeu

Stroka is a government approved food chemist from the university of Wuppertal Germany in 1992

and served as civil servant for the local food authority in Duisburg and Dusseldorf Germany

before he started working for the European Commission in 1996 on a research project within the

Standard Measurement and Testing Program a research project within the 4th research Framework

Program of the European Commission

During his career he has contributed to the development of a number of analytical methods that became international

standards mainly with AOAC as well as other standardization bodies For his contributions to the scientific community

he was awarded by AOAC as Associated Referee of the year in 1999 and Study Director of the year in 2004 He also was

awarded with the Bruno Rossmann price by the German Chemical Society in 2000 for the development of a simplified

and fully semi-conductor based aflatoxin detector He currently leads a small research group including 3 post-doc

scientists His group focusses currently on the development of new methods with a focus on multi-mycotoxin

methods The design and conduction of proficiency tests is another pillar in the work program of the group as well as

training programs for EU National Reference Laboratories

Plant toxin amp Food Adulteration Plant toxins are long known as potential threats for human and animal health Until now the occurrence of food and

feed adulteration has been regulated in Europe by the microscopic Identification of foreign seeds or by the regulation

of certain varieties of crops that are low in the toxins of concern such as potatoes or rapeseeds Toxicological

evaluations by the European Food Safety Authority for some plant toxins triggered the development of analytical

methods suitable for future standards This presentation gives an overview of the currently discussed plant toxins of

concern including some historical background information while stressing the importance of fit-for-purpose methods

based on some examples as they have occurred for the proper estimation of plant toxins by chemical-analytical means

163 participants

25 countries

Dr Xenia Trier Division of Food Chemistry Technical University of Denmark E-mail xttrfooddtudk

Xenia Trier is an analytical research chemist and advisor on analysis of food contact

materials at the National Food Institute at the Technical University of Denmark She

has 18 years of experience in analysis advisory and enforcement testing of food

contact materials for industry and the Danish food and environmental authorities

Her main work has been on the identification and accredited quantification of

migration from plastics paper and board by use of chromatography coupled to

target and semi-non-target accurate mass spectrometry She has more than 25

publications in peer-reviewed journals and as reports Since 2006 her main focus

has been on the development of methods to monitor fluorosurfactants in paper and

board which was the topic of her PhD (2011) Currently she is involved in national

and international research projects on quantification identification risk assessment

and life cycle analysis of individual and cocktails of chemicals in food contact

materials and in human blood

18

The challenge to combine exploratory and confirmatory research Monitoring fluorinated substances

in food packaging and blood by online SPE-LC-ESI-QTOF MS

With the introduction of accurate mass spectrometry enforcement laboratories have acquired new tools to explore

which chemicals are present in low levels of eg materials food and humans with the promise to better characterize

potential risks of contaminants in food Meanwhile quantitative confirmatory data based on validated analytical

methods is required as input for risk assessment which informs risk managers Is it therefore possible to combine the

exploratory non-target analysis with the confirmatory target analysis and what are the implications for the method

validation ndash and the risk assessment

This talk presents the method development and validation for the quantification of 29 and screening of a total of 70 per

and polyfluorinated alkyl substances (PFAS) in paper and board food contact materials and human serum using an

Agilent 126012906550 online SPE-UHPLC-ESIndash-QTOF MS system and an in-house PCDL library Based on three Danish

surveysenforcement campaigns the challenges and possible solutions to verify substances at LOD levels is discussed

and how this needs to be taken into account during the method development As the number of substances increase

in the multi-methods so does the need to optimize the data handling for both quantification and identification This

will be discussed in relation to the use and access to trustworthy accurate mass spectral libraries automated reporting

functions and options for future software improvements

Dr Jens Kirk Andersen PhD in Food Microbiology is a Senior Adviser at the

National Food Institute the Technical University of Denmark

E-mail jkiafooddtudk

He acts as an adviser for the Danish Veterinary and Food Administration on issues and food

safety and food hygiene and food quality He has since 2001 supported the Danish Veterinary

and Food Administration in international fora in the Nordic countries in the EU and in Codex

He has been the training coordination of numerous courses on microbiological criteria

internationally (EU and Asia) He is a general food microbiologist with a special interest in

cold tolerant microorganisms Listeria and Yersinia microbiological criteria and meat

inspection


Dr Taran Skjerdal Norwegian Veterinary Institute

E-mail taranskjerdal vetinstno Taran Skjerdal works as senior researcher at the Norwegian Veterinary Institute (NVI)

with Listeria monocytogenes risks in seafood meat dairy and combined ready-to-eat

products as current main research area She has coordinated several national and

European research projects and is responsible for the national reference functions of

Listeria monocytogenes and coagulase positive Staphylococci in food Before she started

at NVI she worked at the Norwegian Institute of Fisheries and Aquaculture Research and

in the company Det norske Veritas (DNV) She holds a PhD in microbiology from the

Technical University of Norway and is currently member of the Scientific Committee for

Food Safety in Norway

Sampling and analytical methods at early process steps to ensure the food safety of final products

using salmon as case study

Taran Skjerdal Elin Reitehaug Tone Fagereng Karl Eckner and Kofitsyo Cudjoe Norwegian Veterinary Institute

The maximum level for Listeria monocytogenes in ready-to-eat foods in the European Food Law is 100 cfug

throughout the shelf life Sampling is normally carried out at the processing step which means that Listeria can grow in

the product from the sampling point until consume The objective of this presentation is to show how growth after

sampling can be taken into account without compromising the overall criteria using studies of salmon intended used

as sushi as example

The first step is to define the maximum level of L monocytogenes at the step in the process chain the sampling is

carried out which means to predict the growth of Listeria from the sampling point until the product is consumed at

reasonably foreseeable conditions This can be done using challenge studies with artificially contaminated samples

storage of naturally contaminated samples or by using predictive mathematical models For salmon intended used as

sushi the maximum concentration was estimated to 2 cfug

The detection level for standard quantitative methods for L monocytogenes is 10 cfug in 10 grams of sample which

indicates that more sensitive methods are needed for analyses at early process steps Furthermore the studies showed

that at least seven samples of 10 grams each were needed due to unevenly distribution of Listeria within the batches

More sensitive methods and concentration of the sample using either less diluent or normal amount of diluent

combined with MPN or filtration were tested the two former with good results Abuse temperature during transport

of samples was identified as a source of overestimation of Listeria

Concluding remarks Sampling at early process steps based on criteria set up for the last day of shelf life is possible but

performance objectives at early process steps have to be defined for each product and analytical methods need to be

adapted

19

Dr Joslashrgen Schlundt ndash Professor Technical University of Denmark

E-mail jorsdtudk

Joslashrgen Schlundt (JS) has a Veterinary Degree (DVM) as well as a PhD from the Royal

Veterinary and Agricultural University in Copenhagen Denmark He has worked nationally on

environmental and food safety issues from 1983 to 1999 during which period he worked for

3 years at the Veterinary Research Laboratory in Harare Zimbabwe From 1999 ndash 2010 JS

was Director of the Department of Food Safety and Zoonoses at the World Health

Organization Geneva JS has participated in the international development of food safety

Risk analysis principles and has overseen the creation of the International Food Safety

Authorities Network (INFOSAN) and the initiation of the first-ever estimation of the global

burden of foodborne diseases In his present job JS continues an active international

including as Chair of the Steering Committee of the Global Microbial Identifier a new

sequence-based system that will revolutionize microbiology

The GLOBAL MICROBIAL IDENTIFIER ndash the future of microbiology

While previously DNA-sequence based techniques relied on the recognition of short pieces of DNA sequence the NGS

(New Generation Sequencing) technologies now puts within reach for ordinary microbiological labs the sequencing of

enormous amounts of DNA code of microorganisms The NGS technology enables a generic platform for Whole

Genome Sequencing (WGS) of all types of microorganisms ie it represents one uniform testing methodology for all

types of microorganisms within all relevant sectors Animal Food Human Health etc Interestingly while future use

of WGS is likely to boom in developed countries an even more dramatic change in developing countries creates a

potential for significant diagnostic leap-frog in these countries NGS is a simple one-size-fits-all tool for diagnosis of all

infectious diseases holding the potential to dramatically improve public and animal health and food safety in

developing countries The Global Microbial Identifier (GMI) initiative was started in 2011 and is an informal global

taskforce of scientists and other stakeholders who share the aim of making novel genomic technologies and

informatics tools available for improved global diagnostics surveillance and research The GMI suggests a global

system to aggregate share mine and translate genomic data for microorganisms in real-time This system could

include a reference database which would be accessed both for single clinical tasks (simple microbiological

identification) as well as for national and international public health surveillance and outbreak investigation and

response The GMI initiative is constructed around an agreed Charter with a Steering Committee which also includes

representatives from WHO FAO and OIE (See Charter and other information at wwwglobalmicrobialidentifierorg )

GMI has held 8 international meetings the latest (GMI8) in Beijing 11-13 May 2015

Listeria-outbreak in Denmark in 2014 Jens Kirk Andersen Annette Perge Charlotta Loumlfstroumlm and Eva Moslashller Nielsen

In 2014 a large outbreak of Listeriosis was detected and solved In total 41 people was diagnosed in connection to this

outbreak of which 17 cases were fatal It was traced to a plant producing meat products that were distributed all

over the country through numerous secondary plants and retailers The use of whole genome sequencing proved to

be a powerful tool in linking patients to the plant In particular rolled sausages (in Danish ldquorulleposlashlserdquo) was found to

be contaminated however samples collected by the Danish Veterinary and Food Administration showed that Listeria

monocytogenes was present in most of the products in relatively low levels

The outbreak illustrated that low levels of Listeria monocytogenes presents a severe risk to consumers when

occurring in products that supports growth and at the same time has a long shelf-life

In Denmark a remarkable increase in the number of cases of listeriosis has been observed over the last 40 years From

about 20 cases annually in the 1980rsquos to about 60 in recent years making Denmark the country in the world with the

highest observed incidence It is also remarkable that the vast majority of cases are found in the elderly part of the

population with about 85 of cases found in the +60 segment There is no clear explanation for this observation

20

Dr Tor Lea Professor PhD Group leader Molecular Cell Biology group Norwegian University of Life Sciences Arings Norway E-mail torleanmbuno

Research background in medical and basic immunology including topics like genetic

markers and idiotypes on human immunoglobulins neoepitopes on complement

activation products immunomagnetic cell separation regulation of intracellular signaling

in T lymphocyte activation immunomodulatory properties of mesenchymal stem cells

and microbe-host interactions in the regulation of inflammation Has published more than

130 scientific papers in peer-review journals and written 4 textbooks in basic and clinical

immunology Has 30 years of experience as lecturer at Norwegian universities

Microbiota and the significance for human health

During the last decade there has been a surge of interest in our bodyrsquos microbiota particularly the intestinal

microbiota for the immune system and our health in general Experiments in germ-free mice clearly show that the

absence of intestinal microbiota results in defects of the gut-associated immune system with less developed secondary

lymphoid tissues and lower levels of circulating immunoglobulins Additionally the absence of a diverse microbiota has

consequences for the development of other organ systems including the central nervous system Many of these

findings are explained by the intestinal microbiota interacting with host pattern-recognition receptors (PRR) found on

the epithelium and different immune cells of the gut mucosa Thus the microbiota is involved in the maintenance and

development of innate and adaptive immune responses in mucosal tissues and also systemically Moreover changes in

the composition of the gut microbiota are associated with chronic inflammatory conditions like inflammatory bowel

diseases (IBD) type 2 diabetes and metabolic syndrome allergies and autoimmune diseases

(Continued on page 22)

21

Dr Annica Tevell Aringberg National Veterinary Institute (SVA) Sweden

E-mail annicaabergsvase

Annica Tevell Aringberg PhD M Sci Pharm is a senior research scientist at the

Department of Chemistry Environment and Feed Hygiene of the National Veterinary

Institute (SVA) in Uppsala Sweden She is experienced in bioanalysis method

development and method validation primarily with mass spectrometric detection The

last few years the work has mainly been focused on detection of both small toxins such

as mycotoxins in food and feed and large bacterial toxins such as botulinum

neurotoxins in biological matrices food and feed

Microbiological examinations with MALDI-TOF

Mass spectrometry (MS) is a powerful analytical tool used in an increasing number of laboratories all over the world

Since the introduction of the soft ionization techniques the use of MS for the detection of intact macromolecules has

been possible Today MALDI-TOF-MS (matrix-assisted laser desorption ionization time-of-flight MS) is used in clinical

laboratories for fast and cost-effective identification of aerobic and anaerobic bacteria mycobacteria and fungi This

talk will be an introduction to MALDI-TOF-MS detection its applicability and its future possibilities in the field of food

and feed

Dr Gail Betts Section Manager Microbiology Campden BRI Gloucestershire

UK E-mail gailbettscampdenbricouk

Dr Gail Betts has worked at Campden BRI since 1984 and currently manages the

Microbiological Safety amp Spoilage section within the Microbiology Department Originally

starting in the Heat Resistance Group before moving to the Processing and Preservation

Group she has gained over 30 years experience in all aspects of microbial survival and has

written many successful Guidelines documents on Shelf-life and Challenge testing Gail

manages work on predictive food microbiology growth and survival of spoilage organisms

and food pathogens and use of traditional and novel food preservation systems A key area

of her work involves assessing the safety of food products with respect to Listeria

monocytogenes as specified in EC Regulation 2073 In addition for the last 6 years Gail has

managed several studies on the validation of Alternative testing methods according to the

requirements of EN ISO 16140 and she currently sits on the MicroVal Technical Committee



E-mail CharlottaEngdahlAxelssoneurofinsse

Charlotta Engdahl Axelsson studied chemistry and biology at the University of Uppsala

and obtained a PhD in microbiology at the University of Agricultural Sciences in Uppsala

in 1993 She has been working as microbiologist and a Quality Manager for the Food

Industry and as a R amp D Manager for micro- and molecular biology for AnalyCen Nordic

AB Her present position is Group Quality Manager for Eurofins Food amp Feed Testing

Sweden Charlotta is a microbiological expert of NMKL and involved in standardisation of

microbiological methods at CEN and ISO levels a member of validation technical

committees and a board member of EurachemEurolab Sweden

Verification of microbiological methods Today several analytical methods exist for the same microorganismgroup of microorganisms of relevance to foods

In addition to standard methods or other methods published by a recognised organisation there are many

alternative methods validated according to an official protocol It is important that a laboratory verifies the

performance of a method before the method is taken into use for routine testing This includes evaluation of

relevant performance characteristics If the method has previously been externally validated according to an

officially accepted protocol a full validation study is not necessary but performance characteristics depending on the

laboratory eg sample typesmatrices operators equipment media etc should be evaluated

The presentation cover aspects of performance characteristics of relevance for verification of qualitative and

quantitative analytical methods the importance of verifying representative and challenging matrices the number of

samples that should be included in the verification inoculation levels and acceptance criteria eg in relation to level

of detection A process for verification from the description of the method to the implementation in the laboratory

is given Challenges in verification of microbiological methods are compared to challenges in verification of chemical

methods

The collective genome of the gut microbiota represents nearly 200 times as many genes as the human genome

among them genes responsible for the production of metabolites such as the B vitamins vitamin K and short chain

fatty acids (SCFA) like acetate propionate and butyrate The SCFAs are important for epithelial barrier function

satiety regulation immune cell function and activity of the gut-brain axis The metabolism of the gut microbiota is the

source for 13 of all low molecular weight compounds in human blood Currently we have limited knowledge of the

microbial metabolome and its importance for human physiology but novel technologies hold promise for the

characterization of this metabolome and its effects on the host organism The lecture will provide an overview of the

significance of the intestinal microbiota for immune responsiveness mucosal homeostasis and health

22

23

Dr Sven Qvist Chair of NordVal International Denmark E-mail svenqvistcom

Sven Qvist holds a degree of Veterinary Medicine and a postgraduate course in food

microbiology from the Royal Veterinary and Agricultural University in Copenhagen Sven Qvist

has been head of the microbiological department at the Danish Meat Products Laboratory

under the Ministry of Agriculture working with microbiological projects and quality programs

for meat products for the home market and export Sven Qvist has for many years been

working with classical microbiological methods within NMKL IDF and ISO Sven Qvist has

followed closely the development and marketing of alternative microbiological methods and

was in 1985 initiator of the Danish validation system (DanVal) He was in 1999 initiator of the

transition of the Danish validation system into the Nordic validation system (NordVal) In 2007

NordVal was transferred NMKL with its secretariat placed in Oslo Sven Qvist has been elected

chairman for both DanVal and NordVal International

Harmonization of the NordVal International Validation Protocol with the new ISO 161402015

Protocol for the Validation of Alternative Microbiological Methods Food borne diseases are of concern for consumers the food industry retail shops restaurants and the authorities

Therefore food safety management systems and regulations have been adopted both on the national level and in the

framework of international organizations such as EU CODEX WTO and WHO Although strong emphasis has been

focused on prevention systems such as HACCP microbiological testing still plays an important role for the control of

foods in national and international trade In fact the globalization in food trade has caused an increased focus on

capacity and rapidity of analytical methods in order to avoid long time storage of especially perishable foods Since

classical microbiological methods cannot cover this need research laboratories have developed an increasing number

test kits fulfilling the requirements of providing rapid results This development has been followed by regulatory

requirements for proof that the rapid methods produce results equivalent to the accepted standard reference

methods International validation organizations such as AOAC AFNOR MicroVal and NordVal International have been

established to provide the necessary documentation to the authorities on the acceptability of the use of rapid

methods During the last decade great efforts have been carried out to harmonize existing validation protocols into an

accepted validation protocol to be followed by all validation organizations This work has resulted in elaboration of the

new ISO 161402015 validation protocol expected to be finally approved and publish in 2015 This should benefit a

worldwide recognition of validations carried out irrespective of the organization providing the validation results The

advantage of having several organizations operating in the market is avoidance of monopolies as a guarantee for fair

validation prices This presentation will focus on the harmonization of the NordVal International validation protocol

with the new ISO 161402015 protocol

Validation of Alternative Methods We live at a time when we have an increasing number of different test methods available to us There are also a great

number of considerations that will influence which test methods a laboratory may wish chose to use in food analysis

The most appropriate method to use may often be the ISO Reference method however it may be preferred to use

other non-reference lsquoAlternativersquo methods for reasons of cost for ease of use or for improved speed to obtain results

In order to have confidence in the reliability of the test results it is important to ensure that the alternative method

chosen has appropriate validation status In the context of food testing ldquovalidationrdquo means lsquoestablishment of the

performance characteristics of a method and provision of objective evidence that the performance requirements for a

specific intended use are fulfilledrsquo Furthermore if the food testing is done to show compliance with EC Regulation

20732005 then the use of alternative methods is only acceptable when the methods are validated against the ISO

Reference method in accordance with the protocol set out in EN ISO 16140 This talk will describe how a validation

study according to EN ISO 16140 is conducted and will describe the different accreditation bodies that can certify

alternative methods as being suitable for use such as NordVal AOAC MicroVal and AFNOR It will also point put any

pitfalls that expert laboratories method manufacturers and end users should watch out for when developing and

using alternative testing methods


Email JakobOttosonslvse

Dr Jakob Ottoson is an Associate Professor in infection biology with a special interest in

health related environmental microbiology eg the behavior of pathogens outside their

hosthost cell He has been a work package leader in several EU-projects such as ldquoFluresist -

Avian influenza virus survival in poultry commodities poultry manure and the environ-

mentrdquo ldquoVirus in Water Scandinavian Knowledgerdquo and now in ldquoAquavalens ndash Protecting the

health of Europeans by improving methods for the detection of pathogens in drinking water

and water used in food preparationrdquo An overarching method of Jakobrsquos research is microbi-

al risk assessment and presently he works at the department of Risk and benefit assessment

at the National Food Agency in Uppsala but todayrsquos talk will mainly relate to the ongoing

large collaborative project Aquavalens

Standardised molecular detection of waterborne pathogens

New approaches are needed to enable rapid determination of the pathogen load of European drinking water sources

and supply systems used for food processing and preparation human consumption and drinking The new approaches

should be based on molecular methods and complement current cultivation based detection of indicator bacteria

Highly standardized methods are essential validated with certified molecular reference material The approaches will

need to address the issue of inhibition of molecular detection and assess the significance of any positive detection

The use of electronic sensors will also be investigated New techniques will result in detailed insight into the pathogen

load hygienic quality and the specific microbial strains responsible for waterborne outbreaks leading to a better

understanding of the sources infectivity and virulence of these strains The efficacy of these new techniques has to be

demonstrated Aquavalens Greek for safe water was started in relation to the FP7 call Microbially safe water for

human consumption and is expected to develop a new uniform Europe-wide approach regarding drinking water

safety supporting the Drinking Water Directive (9883EC) and the EU innovation union More comprehensive faster

assessment of human health risks from water will be beneficial to the industry water companies laboratories

analyzing water and of course European consumers In this talk I will discuss some of the challenges we are facing

and give some insight in new technologies and possibilities for the implementation in relation to water safety plans

Prof Tomas Torroba University of Burgos Spain E-mail ttorrobaubues

PhD in Chemistry (University of Valladolid Spain 1982) Supervisor Prof Angel Alberola

Current job Full Professor in Organic Chemistry Department of Chemistry University of

Burgos Spain (1998-today) In charge as the Dean of the Faculty of Science University of

Burgos from 2000 to 2004 Past jobs Assistant in Organic Chemistry Department

University of Valladolid from 1978 to 1982 Lecturer in Organic Chemistry Department

University of Extremadura Caceres from 1983 to 1998 Research themes Organic

Chemistry of Heterocyclic Compounds in collaboration with Prof Charles Rees Imperial

College London (UK) (1985-2000) Multicomponent reactions in collaboration with Dr

Stefano Marcaccini University of Florence Italy (1984-2012) Current research Chemical

sensors (2005-today) Co-author of 115 research papers Current research SNIFFER

Sensory devices network for food supply chain security From 2013 to 2016 FP7-SECURITY

Coordinator Andre Oliveira TEKEVER ASDS Portugal Participants 8 Groups from 6

European Countries (continued on page 25)

24

Prof Dr Alejandro Cifuentes Head of Foodomics Laboratory

Institute of Food Science Research (CIAL) National Research Council of Spain

(CSIC) Madrid E-mail acifuentescsices

Dr Alejandro Cifuentes is a Full Research Professor at the National Research Council of Spain

(CSIC) in Madrid and Head of the Laboratory of Foodomics He has been Director of the

Institute of Food Science Research and Deputy Director of the Institute of Industrial

Fermentations both belonging to CSIC He holds different national and international awards is

member of the Editorial Board of 12 international journals (including J Chromatogr A J

Pharmaceut Biomed J Sep Sci Food Anal Method and Int J Mol Sci) and Editor of TrAC

Electrophoresis and Current Opinion in Food Science He has published more than 200 SCI

papers 20 books and book chapters and 6 patents His h index is 52 (December 2014) and his

works have received more than 9000 citations Alejandro has given more than 100 invited

lectures in different national and international meetings in Europe Asia America and Oceania

Foodomics Food Science amp Omics Tools in the 21st Century

Nowadays safety quality and bioactivity of foods and food ingredients can be investigated at molecular level

integrating the results obtained from advanced omics platforms (including genomics transcriptomics proteomics and

or metabolomics) as indicated by Foodomics [1-3] In this work we will introduce some current and future challenges

in food analysis to be addressed by Foodomics and next we will present the last results obtained in our laboratory

following a Foodomics strategy to investigate the anti-proliferative effect of dietary polyphenols against human cancer

cells integrating data from metabolomics and transcriptomics [4-7] Our results give new information on how dietary

polyphenols are able to modulate specific pathways in cancer cells providing new evidences at molecular level on the

antiproliferative effect of this type of compounds [4-7]

REFERENCES [1] Cifuentes A (Ed) Foodomics Advanced Mass Spectrometry in Modern Food Science and Nutrition John Wiley amp Sons 2013 ISBN 9781118169452

[2] Garciacutea-Cantildeas V Simoacute C Herrero M Ibaacutentildeez E Cifuentes A Present and Future Challenges in Food Analysis Foodomics Anal Chem 2012 8410150ndash10159

[3] Herrero M Simoacute C Garciacutea-Cantildeas V Ibaacutentildeez E Cifuentes A Foodomics MS-based strategies in modern food science and nutrition Mass Spec Rev 2012 3149ndash69

[4] Valdeacutes A Garciacutea-Cantildeas V Simoacute C Ibaacutentildeez C Ferragut JA Micol V Cifuentes A Comprehensive Foodomics study on the mechanisms operating at various molecular

levels in cancer cells in response to individual rosemary polyphenols Anal Chem 2014 869807-9815

[5] Saacutenchez-Camargo AP Valdeacutes A Sullini G Garciacutea-Cantildeas V Cifuentes A Ibaacutentildeez E Herrero M Two-step sequential supercritical fluid extracts from rosemary with

enhanced anticancer activity J Funct Foods 2014 11293-303

[6] Valdes A Sullini G Ibantildeez E Cifuentes A Garcia-Cantildeas V Rosemary polyphenols induce unfolded protein response and changes in cholesterol metabolism in

colon cancer cells J Funct Foods 2015 (in press)

[7] Valdeacutes A Garciacutea-Cantildeas V Rocamora L Goacutemez-Martiacutenez A Ferragut JA Cifuentes A Effect of rosemary polyphenols on human colon cancer cells Transcriptomic

profiling and functional enrichment analysis Genes Nutr 2013 843-60

25

SNIFFER Sensory devices network for food supply chain security New fluorescent probes for CBR agents

Project SNIFFER envisions the design and development of a network of distributed detection devices capable of rapid

on-site detection of multiple kinds of agents and CBR agents with high sensitivity and specificity throughout the most

vulnerable stages of the food supply chain (such as farms large collection centres wholesalers etc) The project will

address both available sensor technology and new complementary sensor devices that shall be used for the detection

of hazardous CBR agents within the food supply chain The sensor devices to be developed are characterized by their

portability easiness to use and reusability Another important feature of the new device will be its modular design ie

the device is formed by several independent modules (sensors communication device on-board computing etc)

combined through generalized and standardized connections The network of sensor devices will be designed as a

centralized architecture in which all the data from the devices will be sent to a command centre An operator of the

SNIFFER system will also have the ability to remotely control and command the sensor devices using a specific

interface from the command centre To get these objectives we have designed and synthesized new fluorescent and

colorimetric probes with easiness of bonding to a given target species or to metabolites for enzymatic activity

detection and we have functionalized alkyl silanes of the synthesized fluorescent probes to be used in the preparation

of MIPs The fluorescent probes and their silica supported derivatizations have been tailored for the development of

the sensors in order to address the maximum selectivity and sensitivity for the selected CBR targets A report of the

achievements of this part of the project will be presented

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Development of a Direct Competitive ELISA for the Detection of Nitrofuran Metabolites

in foodstuff of animal origin

ES Vylegzhanina IS Nesterenko (iranes2607gmailcom) KM Filippova AA Komarov AN Panin

The All-Russian State Center for Quality and Standardization of Veterinary Drugs and Feeds

123022 Zvenigorodskoe shosse 5 Russia Moscow

Furazolidone (FZ) and Nitrofurazone (NFZ) are a synthetic broad-spectrum antibiotics used widely as a feed

additive for the prevention and treatment of gastrointestinal infections in swine poultry bees and cattle

The use of nitrofurans has been prohibited completely in food animal production in the European Union

(EU) However it is still widely used in Russia In Russia the MRPL for nitrofuran metabolites residues is 1 μg

kg-1

A polyclonal antibody-based direct competitive ELISA was developed for the determination of tissue-bound

NFZ and FZ metabolites The limits of detection (LOD) calculated from the analysis of 20 known negative

samples (milk honey) were 1 μg kg-1 Recoveries of AOZ and SEM fortified samples ranged from 60 to 120

The coefficients of variation were less than 20 The linear detection range was between 07 and 100 μg L-1

for both compounds All results were submitted by HPLC-MS

Multi Mycotoxin Analysis Using Immunoaffinity Column Clean-Up For A Range of

Samples Prior To LC-MSMS detection

J Wilcox D Leeman E Marley C Milligan amp R Niemeijer R-Biopharm Rhocircne

R-Biopharm Rhonersquos immunoaffinity columns offer a versatile solution for multi mycotoxin analysis whereby

the immunoaffinity columns can be used in tandem with one another to cover the regulated mycotoxins

applicable to a particular food matrix

AOF MS-PREPreg and DZT MS-PREPreg immunoaffinity columns were tested in tan

dem to determine the applicable mycotoxins (total aflatoxin ochratoxin A fumonisin deoxynivalenol

zearalenone T-2 and HT-2) in a number of cereals and cereal based products The samples were analysed

using a single extraction followed by immunoaffinity clean-up with the columns connected in tandem The

eluted solution was analysed by LC-MSMS with a multi mycotoxin method

Matrices analysed were maize based infant food beer bread and breakfast cereal Samples were spiked at

legislative levels and recoveries all met EU method performance criteria For infant food average recoveries

were as follows DON - 106 AFT G2 ndash 74 AFT G1 ndash 90 AFT B2 ndash 83 AFT B1 ndash 78 FUM B1 ndash 90

FUM B2 ndash 84 HT-2 ndash 112 T-2 ndash 90 OTA ndash 62 and ZON ndash 91

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Validation Of A Method For The Analysis Of Sterigmatocystin In Cereals Using

Immunoaffinity Columns P Brown E Marley amp C Milligan R Niemeijer R-Biopharm Rhone

Sterigmatocystin is a precursor in the metabolic pathway for aflatoxin formation and like aflatoxin can

be produced by several Aspergillus species The main producer is A versicolor which is ubiquitous in

nature and has been found to grow on corn bread dried fruit cheese rice and fermented meat

products Although there are many reports on the occurrence of sterigmatocystin in various

commodities many of the thin layer chromatography methods that were traditionally used lack

adequate specificity

In March 2013 the European Food Safety Authority (EFSA) issued a tender for surveillance work on

sterigmatocystin in a variety of grains including wheat barley rye oats and rice intended for human

consumption from 3 different European countries R-Biopharm Rhone has developed an

immunoaffinity column which selectively isolates and concentrates the mycotoxin from a range of

cereal samples The result is better clean up leading to improved chromatography and ultimately

lower limits of detection

Validation of a Test Method for Detecting Pathogenic E coli O157 in Coconut Water Lilian Kuster Philipp Gruber Meredith I Sutzko Zheng Jiang Elisabeth Halbmayr-Jech

Romer Labs Division Holding GmbH Technopark 1 3430 Tulln Austria

Beef dairy and plant products as well as unpasteurized fruit juices have been implicated in numerous

outbreaks of infection by Escherichia coli (specifically O157) worldwide The aim of the study was to

evaluate the performance of the RapidChekreg E coli O157 test system against a cultural reference

method (USDA MLG) for the detection of E coli O157 in coconut water Thirty samples were analyzed

by both methods The sensitivity and specificity of the test system was 100 There were no false

positive or false negative results According to the chi-square analysis (X2 = 108) there was no

significant difference between test and reference method The target pathogen can be detected at

very low levels of contamination in as few as 8 hours with the RapidChekreg test system The test system

allows food producers a simple cost-effective and reliable method to ensure their product is free of

pathogenic E coli O157 serotypes

Validation of the most efficient Pistachio and Cashew ELISA test kits on the market

Lilian Kuster Philipp Gruber Lukas Frank Adrian Rogers Tobias Hein Martin Roumlder Andrea Klink

Romer Labs Division Holding GmbH Technopark 1 3430 Tulln Austria Guelph University

The increasing awareness for food allergens enhancing the allergen testing volume forces food

producers as well as third party testing labs to look for faster but still reliable and accurate detection

methods The fastest allergen ELISA on the market the AgraQuantreg Plus combines a very fast and

convenient sample extraction with short ELISA incubation times of three times 10 minutes Guelph

University (Ontario Canada) was validating two AgraQuantreg Plus kits Cashew and Pistachio on

different quality parameters using the matrices granola ice cream and pepperoni The results in this

validation proved that the AgraQuantreg Plus Cashew and Pistachio are not only capable of providing

results in an extremely fast way but also yield accurate results comparable to well established allergen

ELISA kits on the market making them suitable tools for the detection of allergens in every kind of

foodstuff

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Providing users with the comfort of flexibility Establishment of a cut-off level kit for

allergen lateral flow devices

Lilian Kuster Philipp Gruber Lukas Frank Adrian Rogers

Romer Labs Division Holding GmbH

One major challenge when testing for the presence of food allergens are the missing official threshold

levels leaving it to food manufacturers to set their own internal threshold levels Because of this many

food producers already follow the VITAL system which helps them defining threshold levels for each

allergen which are also depending on the size of the product

But looking at the huge range of food allergen strip tests which are mainly used by food producers due to

their ease of use you will find different degrees of sensitivity only providing one single cut-off level for each

allergen at a time To provide more flexibility to the customer a cut-off level kit has been developed that

can be used in combination with every AgraStripreg Allergen test kit to obtain threshold levels of 5 10 and 20

ppm The validation carried out in conjunction with 5 allergen kits proves the feasibility of the new cut off

level kit

A Versatile Approach to the Analysis of Hormones in Drinking Water using SPE and

LCMSMS

Xianrong (Jenny) Wei (JennyWphenomenexcom) Sean Orlowicz (SeanOphenomenexcom)and Allen

Misa Phenomenex 411 Madrid Ave Torrance CA 90501

Hormone contamination in various water sources including our drinking supply is a growing global concern

As the scientific community tries to identify acceptable exposure limits many new endogenous and

synthetic hormones are being discovered in water supplies Compounds such as ethynylestradiol the

active ingredient in commonly prescribed birth control medication are known to cause detrimental effects

to both aquatic and human life Many other compounds are currently being investigated Due to this public

risk there is a rapidly growing interest in monitoring these compounds

This work highlights an optimized LCMSMS method exploring ionization polarities as well as high pH

mobile phases to maximize MS responses By utilizing an SPE-tube format and exploring various particle

sizes samples from differing drinking water sources can be scaled and processed more easily In addition

the final concentration levels of the extracts can be monitored and controlled to be more appropriate for a

variety of different detectors depending on required sensitivity We present an expanded list of target

compounds to include compounds of current interest (ie progesterone) a modernized extraction and

separation method all while maintaining a short run time with a single injection

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EU FP7 SNIFFER (2013 - 2016)

PhD Elisabeth Henie Madslien Senior Researcher DVM PhD ( Elisabeth-HenieMadslienffino)

Protection and Societal Security Division Norwegian Defence Research Establishment (FFI) PO Box 25 NO

-2027 Kjeller Norway

Project SNIFFER envisions the design and development of a network of distributed detection devices

capable of rapid on-site detection of multiple kinds of agents and CBR agents with high sensitivity and

specificity throughout the most vulnerable stages of the food supply chain (such as farms large collection

centres wholesalers etc) The project will address both available sensor technology and new

complementary sensor devices that shall be used for the detection of hazardous CBR agents within the food

supply chain The network of sensor devices will be designed as a centralized architecture in which all the

data from the devices will be sent to a command centre An operator of the SNIFFER system will also have

the ability to remotely control and command the sensor devices using a specific interface from the

command centre

Simultaneous detection of a broad range of mycotoxins in animal feed with a biochip

platform

Devlin R Plotan M Porter J Hughes L McConnell RI FitzGerald SP

Randox Food Diagnostics 55 Diamond Road Crumlin BT29 4QY United Kingdom

Introduction Biochip array technology (BAT) allows the simultaneous screening of multiple analytes from a

single sample This consolidates testing and greatly reduces the quantity of samples to be assessed by

confirmatory analysis This study reports the applicability of BAT to the simultaneous determination of

multiple mycotoxins from a single animal feed sample

Methodology Competitive chemiluminescent immunoassays arrayed on the biochip surface and applied to

the Evidence Investigator analyser were employed Mycotoxins were extracted from feed by a generic

liquidliquid extraction

Results Aflatoxins ochratoxin A fumonisins trichothecenes zearalenone ergot alkaloids and paxilline

were detected The detection limits were at and below the regulatory limits in feed Initial authentic feed

sample comparisons (n=8) with LC-MSMS showed 100 agreements for all analytes

Conclusions The results show applicability of BAT to the simultaneous screening of multiple mycotoxins

from a single feed sample which increases the screening capacity

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Selective removal of fat for improved pesticide analysis of fat-rich fruits and vegetables Frank Michel1 Emily Barrey2 Katherine K Stenerson2 Olga Shimelis2 Leonard M Sidisky2 1 Sigma-Aldrich Eschenstr 5 82024 Taufkirchen GER frankmichelsialcom 2 SupelcoSigma-Aldrich

595 North Harrison Road Bellefonte PA 16823 USA

The concept of dispersive solid phase extraction (dSPE) clean-up (otherwise known as QuEChERS method) for

the analysis of pesticide contaminants in food samples has been adopted in food testing laboratories due to

the fact that it is a fast and effective sample preparation method After the extraction of the analytes by an

organic solvent usually acetonitrile and phase separation using salts andor buffers the method employs

dispersive SPE for sample clean-up The current SPE phases used in QuEChERS procedure include primary-

secondary amine (PSA) for the removal of acids polar pigments and sugars C18 for the removal of lipid

components and graphitized carbon black (GCB) for the removal of pigments such as chlorophyll Especially

fat-rich fruits and vegetable exhibit a challenge because with the removal of fat non-polar pesticides could

be compromised too

This work presents the application of two new adsorbents for QuEChERS that are based on zirconia-coated

silica Due to the zirconia chemistry both materials show Lewis acid character The zirconia-coated silica

performs better than C18 in model solutions for the removal of fats including glycerides and phospholipids

These materials were applied for the sample clean-up of challenging fat-rich matrices such as olives or

avocado Final analysis of pesticides is conducted by GCMS or LCMS The clean-up and the recovery of

pesticides was evaluated and compared with the traditional sorbents applied in QuEChERS

Advances in the Sample Preparation for Mycotoxin Analysis Frank Michel1 Olga Shimelis2 Ken Espenschied2 Dave Bell2 Emily Barrey2 Matilal Sarker2 Michael Ye2

Michael Halpenny2 1 Sigma-Aldrich Eschenstr 5 82024 Taufkirchen GER frankmichelsialcom 2 SupelcoSigma-Aldrich 595

North Harrison Road Bellefonte PA 16823 USA

Mycotoxins are toxic secondary metabolites produced by fungi which can exist in food as a result of fungal

infection of crops Their strong resistance to decomposition and digestion cause mycotoxins to remain in

the food chain in meat and dairy products The analysis of mycotoxins in food and animal feed has been a

challenge mainly due to the complexity of food matrices and desired low detection limits Immunoaffinity

SPE cartridges have high selectivity for mycotoxins but they are expensive and the procedure involves

multiple steps during cleanup In this study we investigate a line of new SPE materials that are designed

for sample preparation of mycotoxins in complex food matrices such as grains and grain products The

materials are stable and rugged under common laboratory conditions The proposed methods require

homogenization of the matrix blending of the sample with extraction solvent and then passing an aliquot

of supernatant through SPE cartridge

Performance of the SPE products was evaluated for aflatoxins as compared to immunoaffinity clean-up

Recoveries ranging from 101 - 108 were obtained for the new SPE material and 78 - 101 for the

immunoaffinity column The analysis for Deoxynivalenol (DON) was also conducted using the new SPE

material with corn wheat and peanut matrices Recoveries for DON were 79 - 90 for the various

matrices Ochratoxin was also extracted with SPE and average recovery was 108 These new SPE materials

have demonstrated a faster and easier alternative sample preparation method for the quantitative analysis

of mycotoxins in various grains and grain products

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Rapid Detection of Clostridium perfringens by a New Chromogenic Media

Frank Michel1 Mohammad Manafi2 Jvo Siegrist3

1 Sigma-Aldrich Eschenstr 5 82024 TaufkirchenDE frankmichelsialcom 2 Medical University

Vienna Kinderspitalgasse 15 1095 ViennaAT 3 Sigma-Aldrich Industriestr 25 9470 BuchsCH

The European Directive on drinking water quality has included mCP agar as the reference method for

recovering C perfringens from drinking waters mCP agar is difficult to handle the reaction is reversible

and the cells are killed by the ammonia evaporation In the presented study three media (mCP TSCF

and CP ChromoSelect Agar) were evaluated for recovery of C perfringens in different surface water

samples Out of 139 water samples using a membrane filtration technique 131 samples (942) were

found to be presumptively positive for C perfringens in at least one of the culture media Green colored

colonies on CP ChromoSelect Agar (CCP agar) were counted as presumptive C perfringens isolates Out

of 483 green colonies on CCP agar 963 (465 strains indole negative) were identified as C perfringens

15 strains (31) were indole positive and were identified as C sordelli C bifermentans or C tetani Only

3 strains (06 ) gave false positive results and were identified as C fallax C botulinum and C tertium

Variance analysis of the data obtained shows statistically no significant differences in the counts

obtained between media employed in this work The mCP method is very onerous for routine screening

and bacterial colonies could not be used for further biochemical testing The colonies on CCP and TSC

were easy to count and subculture for confirmation tests TSC detects sulfite-reducing clostridia

including species other than C perfringens and in some cases excessive blackening of the agar frustrated

counting of the colonies If the contamination was too high TSC did not consistently produce black

colonies and as a consequence the colonies were white and gave false negative results The

identification of typical and atypical colonies isolated from all media demonstrated that CCP agar was the

most useful medium for C perfringens recovery in water samples

PAHs in Olive Oil Extracting Non-polar Compounds from a Non-polar Matrix using a new

Dual-Layer SPE Cartridge

Frank Michel1 Katherine K Stenerson2 Olga Shimelis2 Ken Espenschied2 Michael Halpenny2

1 Sigma-Aldrich Eschenstr 5 82024 Taufkirchen GER frankmichelsialcom 2 SupelcoSigma-Aldrich


Olive oil can become contaminated with polyaromatic hydrocarbons (PAHs) through exposure of the

olives to pollution in the environment Concern over exposure to these compounds has resulted in

European Union Commission Regulation No 8352011 This regulation sets a maximum limit for PAHs in

edible oils of 2 ngg benzo[a]pyrene alone and 10 ngg in total for the sum of benzo[a]pyrene plus three

additional PAHs Trace analysis of PAHs is commonly done by either GC-MS or HPLC-FLD Oilyfatty

samples present an analytical challenge due to the heavy matrix effects often encountered In the case of

GC-MS fatty matrix can cause contamination of the GC inlet column and detector In the case of HPLC

matrix can build up on the column resulting in loss of chromatographic efficiency andor an increase in

system backpressure Various cleanup techniques exist for fatty samples and some can be time

consuming and expensive In this work a new SPE cartridge containing two different adsorbent layers was

evaluated in the simultaneous extraction and cleanup of PAHs from olive oil The layers consist of Florisil

and a mix of zirconia-coated silicaC18 modified silica Olive oil samples were loaded directly onto the SPE

cartridge followed by elution of the PAHs with acetonitrile while fatty matrix remained bound to the

adsorbents The resulting extract was concentrated and analyzed by both GC-MS and HPLC-FLD The dual-

layer SPE cartridge was evaluated with olive oil samples spiked with light and heavy PAHs and found to

yield recoveries of gt70 and RSD values lt15 for most compounds

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Rapid immunoassay for raw and heat-treated bovine milk proteins in the milk of other species and sources Marco Oteman1 Willem Haasnoot2 (willemhaasnootwurnl )and Piet van Wichen1 1 EuroProxima BV Beijerinckweg 18 6827 BN Arnhem the Netherlands 2 RIKILT Wageningen UR (Institute of Food Safety) Akkermaalsbos 2 6708 WB Wageningen the Netherlands

Monoclonal antibodies (Mabs) were raised against bovine κ-casein an important milk protein on the surface of casein micelles The corresponding epitopes and the dominant amino acids (AAs) were found with ultrahigh-density peptide microarrays by which the specificity towards other milk proteins of cows and other species and sources could be predicted Of one of these Mabs the corresponding 5 AAs-containing epitope was found to be similar in κ-casein from cows and buffalos and not present in other milk proteins of other species and sources and this was confirmed with the rapid ELISA Due to the small epitope and the applied inhibition assay format the ELISA also works with denatured proteins in heat-treated milk which is a unique feature compared to other immunoassays As the epitope is located on the glycomacropeptide part of κ-casein the ELISA also works for the detection of bovine rennet whey (powder) a by-product of cheese production and the cheapest milk product added to bovine milk and to the milk of other species and sources

Recent Developments in High Resolution Mass Spectrometry for the Analysis of

Chemical Contaminats and residues in Food and Feed

Richard Fussell Thermo Fisher Scientific

The monitoring of chemical contaminants and residues in food is usually based on targeted analysis of a

limited number of pre-selected analytes using a combination of LC-MSMS and GC-MSMS A disadvantage

of this approach is that analytes present in the sample but not included in the predefined list will not be

detected A number of high profile cases where these false negative results have come to light at a later

date undermine consumer confidence Thus laboratories are under pressure from consumers and

regulators to detect as many analytes as possible at low concentrations in complex matrices within an

acceptable short timescale and within reasonable cost One possible solution is the use of high-resolution

mass spectrometry with non-targeted acquisition to acquire information on all compounds that can be

chromatographed ionised and detected across the mass range of interest (mz 50-1200) The continued

development of Mass Spectrometry instruments with ever increasing resolving power and sensitivity faster

data acquisition and improved robustness has meant that screening quantification and identification in a

single analysis is an ever closer reality This presentation will highlight the advancements made in in the

field of pesticides and veterinary drugs in Food and animal feed samples

Rapid automatic analysis of acids using a discrete analyzer Mari Klemm Leena Kaski Sari Hartikainen and Annu Suoniemi-Kaumlhaumlrauml (annusuoniemi-

kaharathermofishercom)

Organic acids such as acetic lactic malic citric and tartaric acids provide a characteristic taste to wines

Quantitative acid measurements are typically performed as part of process control during the inspection of

incoming material or as quality control Organic acid analysis also plays a fundamental role in testing the

authenticity of fruit juices The Thermo Scientifictrade Gallerytrade and Arenatrade discrete analyzers used in this

study can automatically measure several acids from a wide range of sample types including wine juice and

beer Homogenous samples can be measured without any pretreatment and the methods used for analysis

are either colorimetric or enzymatic Optimized applications require very low reagent volumes which result

in a low cost per test Method principles are presented and include measuring ranges and performance

data Precision studies demonstrate repeatability and reproducibility and also show how quickly data can be

obtained after inserting samples into the analyzers

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Matrix effects in ultra-high-performance-liquid-chromatographyndashtandem mass

spectrometry antibiotic multi-detection methods in food products with animal origins

Andreia Freitas12 Jorge Barbosa12 and Fernando Ramos23 (framosffucpt) 1INIAV-LNIV Laboratoacuterio Nacional de Investigaccedilatildeo Veterinaacuteria Rua General Morais Sarmento

1500-311 Lisboa Portugal 2CEF-Center for Pharmaceutical Studies Health Sciences Campus Pharmacy Faculty University of Coimbra

Azinhaga de Santa Comba 3000-548 Coimbra Portugal 3CNC-Centre for Neuroscience and Cell Biology Health Sciences Campus Pharmacy Faculty University of

Coimbra Azinhaga de Santa Comba 3000-548 Coimbra Portugal

The efficiency of multi-detection screening methods based on liquid chromatography coupled with tandem

mass spectrometry has been proven in recent years However when simultaneously analyzing different

groups of compounds with different physico-chemical properties the specificity of sample preparation has to

be minimized In mass spectrometry this situation can lead to ion suppression or enhancement of signals due

to interferences coming from the matrices This phenomenon was studied to understand the real impact in

routine analysis Matrix interferences were monitored in extracts of milk muscle liver and fish for 40

antibiotics using recently developed and validated multi-detection methods with Ultra-High-Pressure-Liquid-

Chromatography tandem Mass Spectrometry (UHPLC-MSMS) Although a significant dispersion in the results

was observed for most of compounds ion suppression is the major problem that although it does not

compromise the screening methods can prevent the use of multi-detection for confirmation and

quantification of antibiotic residues in food matrices

Rapid Test System for the Detection of Beer-Spoilage Bacteria Frank Michel1 U-M Kohlstock2 Katja Vetter2 Mathias Zachlod2 Jvo Siegrist3

1 Sigma-Aldrich Eschenstr 5 82024 TaufkirchenDE frankmichelsialcom 2 BECIT GmbH Edisonstr 5

06766 WolfenDE 3 Sigma-Aldrich Industriestr 25 9470 BuchsCH

Beer-spoiling microorganisms cause an increase of turbidity and unpleasant sensory changes in beer Since

improved process technology in modern breweries has resulted in significant reduction of oxygen content in

the final product the role of strictly anaerobic bacteria like Pectinatus and Megasphaera has increased The

detection of these organisms traditionally done by incubation on culture medium takes a week or longer

Thus the development of a rapid and specific method for the detection of beer spoilage organisms is needed

A total of 178 beer samples with alcohol contents between 25 and 67 were tested with a molecular

biological test system based on hybridization of rRNA with Biotin labelled capture probe and a DIG labeled

detection probe (sandwich hybridization) This system detects all known beer spoiling microorganism The

beer samples were spiked with L brevis L coryniformis L lindneri Ped damnosus M cerevisiae and Pec

frisingensis in a range between 103 and 106 CFUsample Additional 32 real brewery samples were also

examined with this system The results were controlled on NBB MRS or PYF agar For the development of

further genera and species specific test system experiments were performed with 17 pure bacterial cultures

of most common beer spoiling microorganisms Results show that the test system is able to detect the spiked

beer spoiling microorganism in all beer samples The detection limit differs between Lactobacillus spp and

the other mentioned beer spoiling genera between 104 and 105 CFUsample For a more sophisticated

analysis several other test systems Pectinatus sppMegasphaera spp Pediococcus spp Lactobacillus

brevis L lindneri ldquoL collinoides L rossiae and L backii were developed These optimized systems

were able to detect specific bacterial count between 20 x 104 and 55 x 108 CFUsample without any

significant cross reaction The developed test system is comparable to classical cultivation methods

Development and optimization of new group- and species-specific test systems offers the ability to detect the

most known beer spoiling organisms in one test platform in a time and cost saving manner

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New Certified Reference Materials for Food Analysis

Frank Michel1 Christine Hellriegel2 Alexander Rueck2

1 Sigma-Aldrich Eschenstr 5 82024 TaufkirchenDE frankmichelsialcom 2 Sigma-Aldrich BuchsCH

Certified Reference Materials (CRMs) for chromatographic investigation of organic compounds in food and

beverages were rarely available until now This due to fact that only a very limited number of organic

CRMs are available from the National Metrological Institutes Quantitative NMR spectroscopy (qNMR) has

become an invaluable instrument for exact content assignment and quantitative determination of

impurities [1] 1H-qNMR is a relative primary method because the intensity of a signal is direct

proportional to the number of protons contributing to the signal of interest [2] Therefore the signals of a

sample compound and a reference substance with known content can directly be compared and the

content of the sample compound can be assigned Based on this approach an unequivocal traceability to

international acknowledged reference standards from a national metrological institute e g from NIST is

achieved [3] Chromatographic techniques such as HPLC or GC usually do not provide this traceability

because they require a highly pure standard of the substance to be investigated which is often not

available This work presents the approach of content assignment by High-Performance Quantitative NMR

(HP-qNMRreg) and its application on organic compounds for Food Analysis from different classes such as

amino acids polycyclic aromatic hydrocarbons pesticides antibiotics fatty acids and fatty acid esters

resulting in new Certified Reference Materials for chromatography The high performance qNMR

measurements were performed at maximum accuracy with a 600 MHz NMR instrument under ISO17025

and ISO34 double accreditation resulting in expanded uncertainties between 01 and 05

Improved methodology for the challenging analysis of non‐polar compounds in fat‐rich

food

Frank Michel1 Emily Barrey2 Katherine K Stenerson2 Olga Shimelis2 Leonard M Sidisky2

1 Sigma‐Aldrich Eschenstr 5 82024 Taufkirchen GER frankmichelsialcom 2 SupelcoSigma‐Aldrich


The analysis of non-polar compounds in fat-rich food is challenging because the target analytes often get

co-extracted with the fat resulting in interferences andor sensitivity issues during analysis Typical

examples of such compounds are persistent organic pollutants (POPs) like polychlorinated biphenyls

(PCBs) organo-chlorine pesticides and polyaromatic hydrocarbons (PAHs) This work describes a new

methodology to selectively remove fat from fat-rich food without compromising the analysis of non-polar

compounds This new approach is based on zirconia- coated silica as adsorbent This adsorbent combines

the interesting chemical properties of zirconia with the high surface area of silica and provides improved

recovery rates and reproducibility for non-polar compounds out of fatty matrices

This approach was applied for dispersive SPE (QuEChERS) as well as packed cartridge SPE Application

areas were the analysis of organochlorine pesticides in fat-rich fruits and vegetables POPs from oils fish

and meat

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Extraction of Fumonisins using a water-based extraction method Lilian Kuster1 Philipp Gruber1 Alois Schiessl1 Barbara Cvak1 Michael Zheng2

Romer Labs Division Holding GmbH Technopark 1 3430 Tulln Austria 2Romer Labs Singapore Pte Ltd 3791 Jalan Bukit Merah 08-08 e-CentreRedhill Singapore 159471

The three most abundant Fumonisins are Fumonisin B1 (FB1) Fumonisin B2 (FB2) and Fumonisin B3 (FB3)

and they have been declared as a potential human carcinogen by the International Agency for Research on

Cancer FB1 in particular has been also linked to leukoencephalomalacia and nephrotoxicity in animals

This group of toxins is produced by fungi growing in agricultural commodities and has to be tested for

before grain is used for animal or human consumption A fast and simple extraction method of grain

followed by a quick on-site screening method is necessarily needed Extraction is usually performed using

organic solvents which are harmful for human and the environment To reduce solvents consumption a

water-based extraction method for Fumonisins was developed recently Complete replacement of organic

solvents by using non-toxic aqueous buffer systems for extraction will be demonstrated by this

presentation

Development of a water-based multi-extraction method for aflatoxin fumonisin

deoxynivalenol zearalenone and ochratoxin in food and feed Lilian Kuster1 Barbara Cvak1 Alois Schiessl1 Philipp Gruber1 Jenniefer Zimmerman2

1Romer Labs Division Holding GmbH Technopark 1 3430 Tulln Austria 2Romer Labs Technology Inc 130 Sandy Drive Newark DE 19713 USA

e-mail philippgruberromerlabscom

Approximately 25 of the worldwide cereal harvest is contaminated with naturally occurring toxins

produced by fungi called mycotoxins These secondary metabolites are capable of causing severe disease

and death in both human and animal Mycotoxins that concern food and feed industry most include

aflatoxins fumonisins deoxynivalenol zearalenone and ochratoxins Extraction of these toxins from

agricultural commodities can be done by using water and extraction buffer The crucial step was to develop

one uniform fast and simple method that is capable of extracting aflatoxins fumonisins deoxynivalenol

zearalenone and ochratoxins from the same sample The uniform extraction is suitable for a wide range of

use including rapid screening methods as lateral flow devices or ELISA This presentation will demonstrate

that a unique water-based method is capable of extracting aflatoxin fumonisin deoxynivalenol

zearalenone and ochratoxin simultaniously

Multi-Mycotoxin Testing ndash MycoSpintrade400 Clean-up and Stable 13C-Labeled Internal

Standards improve accuracy and sensitivity in Mycotoxin LC-MSMS Methods

Lilian Kuster Romer Labs Division Holding GmbH Technopark 1 3430 Tulln Austria

email liliankusterromerlabscom

The technology of choice for multi-mycotoxin analyses is LC-MSMS Nevertheless a problem with LC-MS

MS are matrix interferences leading to differences in analyte ionization The application of fully 13C-labeled

internal standards corrects such mass signal intensities between various sample matrices and pure

standard calibrants to ensure qualified analysis results Highly sensitive mycotoxin detection methods are

demanded by EU legislation and the mycotoxin testing food and feed safety market To detect multiple

mycotoxins at these low detection limits a sample clean-up step should be implemented in the method A

novel rapid multi-mycotoxin clean-up was developed by Romer Labsreg The use of 13C-labeled internal

standards in conjunction with the MycoSpinTM 400 multitoxin clean-up allows for a method applicable to

analyze a variety of matrices In summary the MycoSpinTM clean-up in combination with the application of

isotope labelled internal standards account for a better LC-MSMS sensitivity and specificity on complex

sample matrices

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35

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Salmonella detection from stool samples and food products by using a novel fast and

specific isothermal amplification technology SIBAreg Mari Kukkonen Teemu Halonen Jenna Flinck and Juha Saharinen

Orion Diagnostica Oy RD POBox 83 02101 Espoo Finland

E-mail MariKukkonenoriondiagnosticafi

Salmonella carrying animals and humans cause considerable problems in primary production and food

processing industry We developed a fast and accurate nucleic acid based assay for Salmonella carriers with

very low amounts of bacteria in their stool as an alternative to the widely used and time-consuming culture

method We used selective selenite broth for enrichment of Salmonella and an isothermal DNA

amplification method Strand Invasion Based Amplification (SIBAreg) for the detection of the bacteria in stool

By using selenite enrichment our test was able to detect 1 CFU of Salmonella after only 8 hours of

incubation We also tested BPW and RVS broths to be compatible with our test system

Salmonella Carriage assay could offer fast Salmonella carriage screening after 8-10 hours incubation 10

minutes sample preparation and 50 minutes Orion GenReadreg run This test system will also be suitable for

environmental samples animal stool and food products among others

Application of retrospective analysis for the identification of transformation products of

organic residues in food and feed Mariacutea Luz Goacutemez-Peacuterez Roberto Romero-Gonzaacutelez Patricia Plaza-Bolantildeos Joseacute Luis Martiacutenez Vidal

Antonia Garrido Frenich

Research Group ldquoAnalytical Chemistry of Contaminantsrdquo Department of Chemistry and Physics Research

Centre for Agricultural and Food Biotechnology (BITAL) University of Almeria Agrifood Campus of

International Excellence ceiA3 Carretera de Sacramento sn E-04120 Almeria (Spain)Tel +34 950015823

Fax +34 950015483 E-mail address mgp803ualesagarridouales

The aim of this work has been the application of retrospective analysis to search different compounds not

included in the target analysis evaluating benefits and pitfalls To check this procedure Exactive-Orbitrap

which is able to work in full acquisition mode was used and only with a single injection target and

retrospective analysis could be performed After reprocessing 31 positive samples belonging to different

matrices one metabolite and one transformation product (TP) have been identified in honey and animal

feed respectively Retrospective analysis can be a powerful tool to look for TPs in samples previously

analysed being very efficient for wide-scope screening However the lack of standards could increase the

number of false positives decreasing the reliability of the identification process and instruments providing

high sensitivity for a reliable identification using fragments are needed Finally the presence of TPs in

nutraceutical products was also investigated

Semi-targeted and Challenge Proficiency Tests in Food Analysis

Mark Sykes FAPAS Fera Sand Hutton York YO41 1LZ UK E-mail marksykesferagsigovuk

Proficiency tests by their very nature are best suited to routine analyses of target determinands This is

driven by accreditation (to ISO 17025) and by the development of standard methods Recent problems in

food supply have been caused by unexpected contaminants (melamine horse meat almond for example)

With the number of potential contaminants the analytical technology is evolving to meet those challenges

How is it possible to run a proficiency test for a non-routine analysis Here we describe the outcomes of a

variety of semi-targeted and challenge proficiency tests run by FAPAS These include water chemistry food

microbiology challenge tests and semi-blind food chemistry tests (pesticide residues mycotoxins illegal

dyes) and how they are assessed False negative results can be assessed by generating large negative z-

scores

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How to confirm your mayonnaise is a real mayonnaise

Peter Hoos Unilever RampD Vlaardingen The Netherlands E-mail PeterHoosunilevercom

The definition of Real Mayonnaise is described in the EU code of practice and regulated per country on total

fat content and egg yolk content Fat quantification method are controlled methods within all food

laboratories Cholesterol is the most straight forward egg yolk marker in mayonnaise with AOAC 99410 and

JAOAC 97 as typical reference methods Unfortunately due to the mayonnaise matrix it is still difficult to

analyse cholesterol with GC-FID Butter is a suitable cholesterol reference material with comparable difficult

matrix Phospholipids can also be used as egg yolk markers but due to strong emulsifying properties of the

different molecules in combination with the mayonnaise matrix their analysis is even more challenging For

the quantification and confirmation 31P-NMR with single phase extraction is a more obvious analytical

technique to use for phospholipids quantification in mayonnaise To conclude Real Mayonnaise is a difficult

analytical matrix

Developed and validation of a liquid chromatography-tandem mass spectrometry

method for the determination of five dye residues in aquaculture products

Andrea Macaluso1 Vita Giaccone1 Giuseppe Polizzotto2 Angela Alongi1 Antonio Vella1 and Vincenzo

Ferrantelli1 andreamacalusoizssiciliait 1 Istituto Zooprofilattico Sperimentale della Sicilia AMirri

Palermo (Italy) 2 Universitagrave di Palermo Dipartimento STEBICEF (Italy)

A method for the quantitative determination of the triphenylmethane dyes malachite green (MG) leuco

malachite green (LMG) brilliant green (BG) crystal violet (CV) and leuco crystal violet (LCV) in fish muscle

was developed and validated MG is used in aquaculture as fungicide They have been banned due to their

carcinogenic activity by FDA and UE BG was evaluated for the similar chemical structure and the possible

similar toxicity The analytes were extracted from the matrix using a LCLC extraction with a mixture of

acetonitrile and citrate buffer the clean-up was performed using the quechers tecnique and the purified

sample was injected in a LC-MSMS An internal calibration was used In accordance with the Commission

Decision 2002657EC the method has been validated in line with the minimum required performance limit

MRPL of 2 microgKg The validation parameters such as instrumental linearity specificity precision repeatability

and reproducibility recovery decision limit (ccα) detection capabilities (ccβ) and ruggedness were evaluated

with good results for the suitability of the method

EU Reference Laboratory- Campylobacter Proficiency Tests for National Reference

Laboratories Progress of performance in five years Hansson I B Lahti E Nyman A and Olsson Engvall E

EURL Campylobacter National Veterinary Institute Uppsala Sweden

E-mail ingridhanssonsvase

The European Union Reference Laboratory (EURL) for Campylobacter organizes annual proficiency tests for

the National Reference Laboratories (NRLs) with the aim to ensure the quality of Campylobacter analysis In

2010 to 2014 the PTs included detection and enumeration of Campylobacter spp using the standardized

protocols of ISO 10272 The number of participating NRLs were between 34 and 36 The proportion of NRLs

that reported correct results of detection in all samples ranged from 56 to 74 with the highest in 2014

Enumeration was performed by colony count technique and the NRLs that reported correct results ranged

from 65 to 91 Most incorrect results were reported on sample which contained a mixed culture of

Campylobacter and E coli The majority of the NRLs performed excellent or good in detection species

identification and enumeration The EURL- Campylobacter assist the few NRLs that need to improve their

performance for example by training courses

P27

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37

Po

ster A

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ay 2

Soxhlet-based fat analysis reinvented with new total fat solution Janne Pedersen (email JAPfossdk) Foss Analytical AS

FOSS announces the Soxtectrade 8000 extraction unit and Hydrotectrade hydrolysis unit the first fully

automated solution for total fat analysis with the Soxhlet method

Fully automated Soxhlet analysis

Consisting of an extraction unit a hydrolysis unit and a single Hydrocap filter that is common to both units

the Soxtectrade 8000 allows hydrolysis and Soxhlet analysis to be performed in one integrated laboratory

process Other advances in the automation of fat analysis include a solvent dosage dial for improved safety

and minimal operator time Smart water cooling only starts when actually required self adjusting hotplates

ensure optimal heat transfer to extraction cups and the hotplates can be individually switched on an off

Improving throughput at a lower cost

The unique filter system that goes all the way through hydrolysis to final extraction allows users to avoid

filter to filter transfer This saves time and labor and avoids the risk of human error and associated costs An

optional extra six position unit in the extraction phase allows batch handling of up to 12 samples using the

same control unit Plus an automatic shutdown feature allows out of hours operation

Safe operations

Safety features improve on existing automated Soxhlet solutions Features such as the enclosed solvent

dosage system and effective cooling help to avoid contact with solvents chemicals or fumes and the design

of the extraction unit also allows use of a broad range of tested solvents including some that are

considered to be unsafe when used with other solutions

Multi residue analysis of pesticides in Wine using ethyl acetate extraction method Despo Louca Christodoulou Popi Kanari Panayiota Hadjiloizou Panayiotis Constantinou

Pesticide Residues Laboratory of the State General Laboratory

Kimonos 44 Nicosia 1451 Cyprus wwwsglmohgovcy

Ministry of Health (MH) is the competent authority in Cyprus for the control of pesticide residues in

foodstuffs The Pesticide Residues Laboratory (PRL) of the State General Laboratory is the Official Lab for the

Monitoring amp Surveillance of Pesticide Residues in Food and it has been nominated as the NRL of Pesticides

Residues in Food The PRL of the SGL is accredited since 2002 for the analysis of pesticides residues in food

of plant origin including fruits and vegetables

The control of residues in wine is required by the Control Regulation of 2013 Reg (EU) No 7882012

In order to cope with the requirements of the community monitoring programme the ethyl acetate multi

residue method has been expanded for the analysis for more than 170 pesticides in wine

The poster presents the validation of the ethyl acetate extraction method in wine and the results of a survey

study in samples of Cypriot Wine

The validation study was in accordance to the DG SANCO guidelines (1) Included in the scope of validation

were recovery linearity limits of quantification and precision Recoveries of the majority of compounds

were in the 70-120 range and were characterized by precision lower than 20

The validated method was used in a real sample survey carried out on 27 samples of various Cypriot Wine

red white dry sweet The 556 of the real samples analysed found to be positive with pesticides

residues all observed values were much lower than the MRLs

P30

P31

38

List of P

articip

ants

Anne-Lise Groslashholt 3M Food Safety Sweden

Kjell Rehn 3M Food Safety Sweden

Viola Dahl Agilent Technologies Sweden

Agilent Technologies Sweden

Anders Edlund ALcontrol AB Sweden

Gabriella Karp ALcontrol AB Sweden

Gabriella Brusquini Calmervik Alcontrol Laboratories Sweden

Valeacuterie Bardin American Proficiency Institute USA

Aringke Arvidson ANL Produkter Leif Norin AB Sweden

Eric Verdon Anses - Laboratory of Fougeres France

Valerie Gaudin Anses - Laboratory of Fougeres France

Philippe Leroux AOAC Europe France

Heidi Camilla Sagen Aquatic Concept Group Norway

Annie Kaalby Arla Foods Denmark

Tanja Bloszlig BECIT GmbH Germany

Atif Javaid BergmanLabora AB Sweden

Bernd Renger Bernd Renger Consulting Germany

Lukasz Pawelek Bioavlee Poland

Hans Ragnar Norli Bioforsk Plant Sciences Norway

Erling Markussen Biolab AS Denmark

Yannick Bichot BIO-RAD Laboratories France

Daniel Meier Buchi Switzerland

Gail Betts Campden BRI United Kingdom

Kristina Lazarevic Center for Food Analysis Belgrade Serbia

Smiljana Raicevic Center for Food Analysis Belgrade Serbia

Sune Eriksson Chiron AS Norway

Anita Sundgaard Christensen Chr Hansen Denmark

Lene Berg Joslashrgensen Chr Hansen Denmark

Nete Bernbom Chr Hansen Denmark

Eva Vysatova Czech Agriculture and Food Inspection Authority Czech Republic

Radim Stepan Czech Agriculture and Food Inspection Authority Czech Republic

Susanne Mansdal Danish Meat Research Institute Danish Technological Inst

Denmark

Aase Mikkelsen Danish Veterinary and Food Administration Denmark

Birgitte Nauerby Danish Veterinary and Food Administration Denmark

Julia Roman Moeller Danish Veterinary and Food Administration Denmark

Laust Oslashstergaard Danish Veterinary and Food Administration Denmark

Susanne Molboe Danish Veterinary and Food Administration Denmark

Inge Rokkjaeligr Danish Veterinary and Food Administration Aringrhus Denmark

39

List of P

articip

ants

Anne Marie Lassen-Nielsen Danish Veterinary and Food Administration Aringrhus Denmark

Arne Hoslashjgaringrd Danish Veterinary and Food Administration Aringrhus Denmark

Erik Dahm Danish Veterinary and Food Administration Aringrhus Denmark

Mette Bakman Danish Veterinary and Food Administration Aringrhus Denmark

Kepher Kuchana KATEU Directorate of Government Analytical Laboratory Uganda

Kathleen Merx DNA Diagnostik Nord Germany

Nina Skammelsrud Eurofins Food amp Feed Testing Norway AS Norway

Ragnhild Skyrud Eurofins Food amp Feed Testing Norway AS Norway

Laura Tiano Eurofins Steins Laboratory AS Denmark

Charlotta Engdahl Axelsson Eurofins Steins Laboratory AS Sweden

Else Byskov Cavazzi Eurofins Steins Laboratory AS Denmark

Frans Verstraete European Commission Directorate General for Health and Consumers

Belgium

Franz Ulberth European Commission Joint Research Centre Belgium

Joerg Stroka European Commission Joint Research Centre Belgium

Marco Oteman EuroProxima The Netherlands

Joanne Howard Exova UK United Kingdom

Catherine Cockcroft Exova UK Ltd United Kingdom

Craig Eaton FAPAS United Kingdom

Mark Sykes FAPAS United Kingdom

AnnSiri Borg Hentze Faroese Food and Veterinary Authority Faroe Islands

Debes H Christiansen Faroese Food and Veterinary Authority Faroe Islands

Guethrun Hoslashjgaard Faroese Food and Veterinary Authority Faroe Islands

Jogvan P Fjallsbak Faroese Food and Veterinary Authority Faroe Islands

Rikke Larsen Faroese Food and Veterinary Authority Faroe Islands

Vaacuter Roacutein Faroese Food and Veterinary Authority Faroe Islands

Irina Nesterenko FGBU VGNKI Russia

Annika Pihlajasaari Finnish Food Safety Authority Evira Finland

Janne Nieminen Finnish Food Safety Authority Evira Finland

Osman INAY Food Control Lab Turkey

Hanna Tidblom Food Diagnostics Sweden

Karine Bjerre Food Diagnostics Denmark

Marianne Yesmes Food Standards Scotland United Kingdom

Janne Pedersen FOSS Analytical AS Denmark

Maria Wickman FOSS Analytical AS Denmark

Reneacute Fuhlendorff FOSS Analytical AS Denmark

Belinda Flem Geological Survey of Norway Norway

Katriina Luoma HKScan Finland Finland

Elisabeth Blikoslash Labolytic AS Norway

Labolytic AS Norway

Alejandro Cifuentes Laboratory of Foodomics CIAL National Research Council of Spain

Spain

40

List of P

articip

ants

Carl-Johan Arevaringng Larodan AB Sweden

Jan Wahlstroumlm Larodan AB Sweden

Javier Miralles Garcia Mars Petcare France

Margreacutet Geirsdoacutettir Matiacutes Iceland

Bert Popping Meacuterieux NutriSciences Corporation France

Pierre Metra Meacuterieux NutriSciences Corporation France

Suvi Ojanperauml MetropoliLab Ltd Finland

Nina Hostnik Mlekarna Celeia Slovenia

Tanja Veselko Vinko Mlekarna Celeia Slovenia

Alfredo Montes-Nintildeo Murillo Tecnologiacutea SL Spain

Anders Staffas National Food Agency Sweden

Catarina Flink National Food Agency Sweden

Hans Lindmark National Food Agency Sweden

Irina Boriak National Food Agency Sweden

Jacob Ottoson National Food Agency Sweden

Johan Roseacuten National Food Agency Sweden

Laurence Nachin National Food Agency Sweden

Lisa Fredlund National Food Agency Sweden

Rasmus Groumlnholm National Food Agency Sweden

Stig Orustfjord National Food Agency Sweden


Ulla Edberg National Food Agency Sweden

Yeskob Ottoson National Food Agency Sweden

Jens Kirk Andersen National Food Institute DTU Denmark

Stig Valdersnes National Institute of Nutrition and Seafood Research NIFES Norway

Anette H Kausland National Institute of Nutrition and Seafood Research NIFES Norway

Annica Tevell Aringberg National Veterinary Institute Sweden

Ingrid Hansson National Veterinary Institute Sweden

Erik Konings Nestleacute Research Center - Nestec Ltd Switzerland

Marte Monshaugen NMBU Veterinaeligrhoslashgskolen Norway

Olga Anna Osinska NMBU Veterinaeligrhoslashgskolen Norway

Hilde Skaringr Norli NMKL Norwegian Veterinary Institute Norway

Sven Qvist NordVal International Denmark

Astrid Nordbotten Norwegian Food Safety Authority Norway

Jan Alexander Norwegian Institute of Publich Health Norway

Tor Lea Norwegian University of Life Sciences NMBU Norway


Gro S Johannessen Norwegian Veterinary Institute Norway

Taran Skjerdal Norwegian Veterinary Institute Norway

Mari Kukkonen Orion Diagnostica Oy Finland

Susanna Lampo + 1 Phenomenex Aps Sweden 41

List of P

articip

ants

Klaus Reif PhytoLab GmbH amp Co KG Germany

Lisa Murphy Qiagen United Kingdom

Marcia Armstrong Qiagen USA

Lisa Hughes Randox Food Diagnostics United Kingdom

Marta Herrero Randox Food Diagnostics United Kingdom

Christine Maria Gutschelhofer R-Biopharm AG Germany

R-Biopharm AG Germany

Lilian Kuster Romer Labs Division Holding GmbH Austria

Philipp Gruber ROMER Labs Division Holding GmbH Austria

Daniel McMillan SCIEX Germany

Kasper Oland SCIEX Germany

Annika Thoumlrnberg Semper AB Sweden

Flor Khalili Semper AB Sweden

Per Persson Semper AB Sweden

Frank Michel Sigma-Aldrich Chemie GmbH Germany

Maja Lojovic SP Laboratories AD Serbia

Danica Milinkov Guljas SP Laboratories AD Serbia

Thomas Gude SQTS Switzerland

Popi Kanari State General Laboratory Ministry of Health Cyprus

Adia Groza Swedac Sweden

Joslashrgen Schlundt Technical University of Denmark Denmark

Xenia Trier Technical University of Denmark Denmark

Elisabeth Henie Madslien The Norwegian Defence Research Establishment (FFI) Norway

Anders Thomsson Thermo Fisher Scientific United Kingdom

Annu Suoniemi-Kaumlhaumlrauml Thermo Fisher Scientific Finland

Carolyn Pritchard Thermo Fisher Scientific United Kingdom

Cesare Rossini Thermo Fisher Scientific Italy

Karin Loumlnnqvist Thermo Fisher Scientific Finland

Mikko Kauppinen Thermo Fisher Scientific Finland

Per Nilsson Thermo Fisher Scientific Sweden

Richard Fussell Thermo Fisher Scientific Sweden

Anette Westh Lauritsen Thermo Fisher Scientific Denmark

Jonathan Cloke Thermo Fisher Scientific-Oxoid Ltd UK

Peter B Hoos Unilever RampD Vlaardingen The Netherlands

Maria Luz Gomez Perez University of Almeria Spain

Tomas Torroba University of Burgos Spain

Fernando Ramos University of Coimbra Portugal

Anu Surakka Valio Ltd Finland

Ruud J B Peters Wageningen University The Netherlands

Joe Whitworth William reed business media France

Bitte Bamberg Aringlands miljouml-och haumllsoskyddsmyndighet Mariehamn Aringland Finland

Lars Jorhem Sweden 42

Available NMKL Procedures

No 1 2 Ed 2005 Calibration and performance checking of laboratory balances

No 3 1996 Control charts and control materials in internal quality control in food chemical laboratories

No 4 3 Ed 2009 Validation of chemical analytical methods

No 5 2 Ed 2003 Estimation and expression of measurement uncertainty in chemical analysis

No 6 1998 (Adm 2002 Adm 2006) Generelle retningslinier for kvalitetssikring af sensoriske laborato-

rier (only available in Danish and Finnish)

No 7 1998 Checking of UVVIS spectrophotometers

No 8 4 Ed 2008 Measurement of uncertainty in quantitative microbiological examination of foods

No 9 2 Ed 2007 Evaluation of method bias using certified reference materials

No 10 2001 Control of Microbiological Media

No 11 2 Ed 2010 Procedure for sensory analysis of drinking water

No 12 2 Ed 2014 Guide on sampling for analysis of foods

No 13 2003 Volumetric control

No 14 2004 SENSVAL Guidelines for internal control in sensory analysis laboratories

No 16 2005 (2007) Sensory quality control

No 17 2006 Guidelines for requirement specifications for food analyses

No 18 2006 The use of reference materials reference strains and control charts in a food microbiological

laboratory

No 19 2007 Guideline for sensorial Analysis of Food containerspackages

No 20 2007 Evaluation of results from qualitative methods

No 21 2008 Guide for sensory analysis of fish and shellfish

No 22 2008 Considerations regarding evaluation of immunochemical test kits for food analysis

No 23 2008 Guide on quality assurance in microbiological laboratories

No 24 2010 Guidelines for quality assurance for food chemical laboratories

No 25 2014 Recovery information in analytical measurement

No 26 2012 Control and internal calibration of thermometers and temperature control on microbiological

laboratories

No 27 2013 Measurement uncertainty in sensory analysis

No 28 2014 Guidelines for reporting sensory data

No 29 2014 Guidelines for sensory analysis of meat and meat products

No 30 2014 Statistical Evaluation of Results from Quantitative Microbiological Methods

43

44

EUROPE SECTION OF

AOAC INTERNATIONAL

AOAC Europe is a section of AOAC INTERNATIONAL and was

established in 1989 It is an organization of professional

scientists that exchange knowledge and information to help

each other excel in their profession

The activities of the section are of interest to many

stakeholders from industry and trade consumer and

environmental protection agencies public authorities and

regulators in Europe andor Mediterranean countries

wwwaoaceuropecom

NordVal International performs a third-party review and

certifies alternative chemical and

microbiological methods for food

feed water faeces and

environmental testing

NordVal offers

a user-friendly validation

protocol

scientific confirmation policies

specified acceptance criteria

independent and rapid

approval procedures

guidance in the validation

process

a network for analytical experts

reliable analytical methods within chemical

microbiological and sensorial methods

independent reviews and NordVal

International certifications of alternative

methods

relevant guidelines

coursesworkshopsseminars

an updated list of contact persons of the

Nordic national reference laboratories

NMKL offers

wwwnmklorg

The Nordic Committee on Food

Analysis NMKL was founded in

1947 and consists of chemists

microbiologists sensory analysts and

statisticians from the five Nordic

countries Denmark Finland Iceland

Norway and Sweden

NMKL is linked to the Nordic Council

of Ministers

2






















3M Food Safety


BergmanLabora AB

Biolab AS

FAPAS

Food Diagnostics

Foss Analytical AS

Labolytic AS

Larodan AB

Phenomenex Aps


R-Biopharm AG

SCIEX


Exhibitors


Contents

3





Germany



1315 - 1330 Welcome







Safety Norway












1700 - 1705 Closure


4





















1400 - 1430








5





of final products
















1400 - 1430








Plenary 22 May








Council of Spain


Auditorium


Entre

Toilets

Wardrobe

Exhibition area

Conference location

7











Welcome from NMKL
























8

















Committee)







needs























9
















































10


























11


















method



























































in global trade











12



















13

















will be discussed





















presented including





















Honey Commission


14






























15
















drugs in foods

































non-food











16






















17
























163 participants

25 countries















18


























inspection



















as sushi as example














adapted

19


E-mail jorsdtudk











































20





















21
















and feed





































methods









22

23























































































24


































25


















Po

ster A

bstracts D

ay 1










kg-1





















P1

P2

26

Po

ster A

bstracts D

ay 1







































foodstuff

P3

P4

P5

27

Po

ster A

bstracts D

ay 1














level kit


LCMSMS
















P6

P7

28

Po

ster A

bstracts D

ay 1

EU FP7 SNIFFER (2013 - 2016)












command centre


platform















P9

P8

29

Po

ster A

bstracts D

ay 1











be compromised too



























P10

P11

30

Po

ster A

bstracts D

ay 1













































P12

P13

31

Po

ster A

bstracts D

ay 1































P14

P15

P16

32










































P17 Po

ster A

bstracts D

ay 2

P18

33

Po

ster A

bstracts D

ay 2






















food














and meat

P19

P20

34

Po

ster A

bstracts D

ay 2












presentation





























sample matrices

P21

P22

P23

35

Po

ster A

bstracts D

ay 2










































scores

P24

P25

P26

36

Po

ster A

bstracts D

ay 2












analytical matrix































P27

P28

P29

37

Po

ster A

bstracts D

ay 2















Safe operations
























P30

P31

38

List of P

articip

ants

































Denmark







39

List of P

articip

ants













Belgium



























Labolytic AS Norway


Spain

40

List of P

articip

ants










































List of P

articip

ants





























































laboratory









laboratories





43

44






















3M Food Safety


BergmanLabora AB

Biolab AS

FAPAS

Food Diagnostics

Foss Analytical AS

Labolytic AS

Larodan AB

Phenomenex Aps


R-Biopharm AG

SCIEX


Exhibitors


Contents

3





Germany



1315 - 1330 Welcome







Safety Norway












1700 - 1705 Closure


4





















1400 - 1430








5





of final products
















1400 - 1430








Plenary 22 May








Council of Spain


Auditorium


Entre

Toilets

Wardrobe

Exhibition area

Conference location

7











Welcome from NMKL
























8

















Committee)







needs























9
















































10


























11


















method



























































in global trade











12



















13

















will be discussed





















presented including





















Honey Commission


14






























15
















drugs in foods

































non-food











16






















17
























163 participants

25 countries















18


























inspection



















as sushi as example














adapted

19


E-mail jorsdtudk











































20





















21
















and feed





































methods









22

23























































































24


































25


















Po

ster A

bstracts D

ay 1










kg-1





















P1

P2

26

Po

ster A

bstracts D

ay 1







































foodstuff

P3

P4

P5

27

Po

ster A

bstracts D

ay 1














level kit


LCMSMS
















P6

P7

28

Po

ster A

bstracts D

ay 1

EU FP7 SNIFFER (2013 - 2016)












command centre


platform















P9

P8

29

Po

ster A

bstracts D

ay 1











be compromised too



























P10

P11

30

Po

ster A

bstracts D

ay 1













































P12

P13

31

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ster A

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ay 1































P14

P15

P16

32










































P17 Po

ster A

bstracts D

ay 2

P18

33

Po

ster A

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ay 2






















food














and meat

P19

P20

34

Po

ster A

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ay 2












presentation





























sample matrices

P21

P22

P23

35

Po

ster A

bstracts D

ay 2










































scores

P24

P25

P26

36

Po

ster A

bstracts D

ay 2












analytical matrix































P27

P28

P29

37

Po

ster A

bstracts D

ay 2















Safe operations
























P30

P31

38

List of P

articip

ants

































Denmark







39

List of P

articip

ants













Belgium



























Labolytic AS Norway


Spain

40

List of P

articip

ants










































List of P

articip

ants





























































laboratory









laboratories





43

44





Germany



1315 - 1330 Welcome







Safety Norway












1700 - 1705 Closure


4





















1400 - 1430








5





of final products
















1400 - 1430








Plenary 22 May








Council of Spain


Auditorium


Entre

Toilets

Wardrobe

Exhibition area

Conference location

7











Welcome from NMKL
























8

















Committee)







needs























9
















































10


























11


















method



























































in global trade











12



















13

















will be discussed





















presented including





















Honey Commission


14






























15
















drugs in foods

































non-food











16






















17
























163 participants

25 countries















18


























inspection



















as sushi as example














adapted

19


E-mail jorsdtudk











































20





















21
















and feed





































methods









22

23























































































24


































25


















Po

ster A

bstracts D

ay 1










kg-1





















P1

P2

26

Po

ster A

bstracts D

ay 1







































foodstuff

P3

P4

P5

27

Po

ster A

bstracts D

ay 1














level kit


LCMSMS
















P6

P7

28

Po

ster A

bstracts D

ay 1

EU FP7 SNIFFER (2013 - 2016)












command centre


platform















P9

P8

29

Po

ster A

bstracts D

ay 1











be compromised too



























P10

P11

30

Po

ster A

bstracts D

ay 1













































P12

P13

31

Po

ster A

bstracts D

ay 1































P14

P15

P16

32










































P17 Po

ster A

bstracts D

ay 2

P18

33

Po

ster A

bstracts D

ay 2






















food














and meat

P19

P20

34

Po

ster A

bstracts D

ay 2












presentation





























sample matrices

P21

P22

P23

35

Po

ster A

bstracts D

ay 2










































scores

P24

P25

P26

36

Po

ster A

bstracts D

ay 2












analytical matrix































P27

P28

P29

37

Po

ster A

bstracts D

ay 2















Safe operations
























P30

P31

38

List of P

articip

ants

































Denmark







39

List of P

articip

ants













Belgium



























Labolytic AS Norway


Spain

40

List of P

articip

ants










































List of P

articip

ants





























































laboratory









laboratories





43

44





















1400 - 1430








5





of final products
















1400 - 1430








Plenary 22 May








Council of Spain


Auditorium


Entre

Toilets

Wardrobe

Exhibition area

Conference location

7











Welcome from NMKL
























8

















Committee)







needs























9
















































10


























11


















method



























































in global trade











12



















13

















will be discussed





















presented including





















Honey Commission


14






























15
















drugs in foods

































non-food











16






















17
























163 participants

25 countries















18


























inspection



















as sushi as example














adapted

19


E-mail jorsdtudk











































20





















21
















and feed





































methods









22

23























































































24


































25


















Po

ster A

bstracts D

ay 1










kg-1





















P1

P2

26

Po

ster A

bstracts D

ay 1







































foodstuff

P3

P4

P5

27

Po

ster A

bstracts D

ay 1














level kit


LCMSMS
















P6

P7

28

Po

ster A

bstracts D

ay 1

EU FP7 SNIFFER (2013 - 2016)












command centre


platform















P9

P8

29

Po

ster A

bstracts D

ay 1











be compromised too



























P10

P11

30

Po

ster A

bstracts D

ay 1













































P12

P13

31

Po

ster A

bstracts D

ay 1































P14

P15

P16

32










































P17 Po

ster A

bstracts D

ay 2

P18

33

Po

ster A

bstracts D

ay 2






















food














and meat

P19

P20

34

Po

ster A

bstracts D

ay 2












presentation





























sample matrices

P21

P22

P23

35

Po

ster A

bstracts D

ay 2










































scores

P24

P25

P26

36

Po

ster A

bstracts D

ay 2












analytical matrix































P27

P28

P29

37

Po

ster A

bstracts D

ay 2















Safe operations
























P30

P31

38

List of P

articip

ants

































Denmark







39

List of P

articip

ants













Belgium



























Labolytic AS Norway


Spain

40

List of P

articip

ants










































List of P

articip

ants





























































laboratory









laboratories





43

44





of final products
















1400 - 1430








Plenary 22 May








Council of Spain


Auditorium


Entre

Toilets

Wardrobe

Exhibition area

Conference location

7











Welcome from NMKL
























8

















Committee)







needs























9
















































10


























11


















method



























































in global trade











12



















13

















will be discussed





















presented including





















Honey Commission


14






























15
















drugs in foods

































non-food











16






















17
























163 participants

25 countries















18


























inspection



















as sushi as example














adapted

19


E-mail jorsdtudk











































20





















21
















and feed





































methods









22

23























































































24


































25


















Po

ster A

bstracts D

ay 1










kg-1





















P1

P2

26

Po

ster A

bstracts D

ay 1







































foodstuff

P3

P4

P5

27

Po

ster A

bstracts D

ay 1














level kit


LCMSMS
















P6

P7

28

Po

ster A

bstracts D

ay 1

EU FP7 SNIFFER (2013 - 2016)












command centre


platform















P9

P8

29

Po

ster A

bstracts D

ay 1











be compromised too



























P10

P11

30

Po

ster A

bstracts D

ay 1













































P12

P13

31

Po

ster A

bstracts D

ay 1































P14

P15

P16

32










































P17 Po

ster A

bstracts D

ay 2

P18

33

Po

ster A

bstracts D

ay 2






















food














and meat

P19

P20

34

Po

ster A

bstracts D

ay 2












presentation





























sample matrices

P21

P22

P23

35

Po

ster A

bstracts D

ay 2










































scores

P24

P25

P26

36

Po

ster A

bstracts D

ay 2












analytical matrix































P27

P28

P29

37

Po

ster A

bstracts D

ay 2















Safe operations
























P30

P31

38

List of P

articip

ants

































Denmark







39

List of P

articip

ants













Belgium



























Labolytic AS Norway


Spain

40

List of P

articip

ants










































List of P

articip

ants





























































laboratory









laboratories





43

44

Plenary 22 May








Council of Spain


Auditorium


Entre

Toilets

Wardrobe

Exhibition area

Conference location

7











Welcome from NMKL
























8

















Committee)







needs























9
















































10


























11


















method



























































in global trade











12



















13

















will be discussed





















presented including





















Honey Commission


14






























15
















drugs in foods

































non-food











16






















17
























163 participants

25 countries















18


























inspection



















as sushi as example














adapted

19


E-mail jorsdtudk











































20





















21
















and feed





































methods









22

23























































































24


































25


















Po

ster A

bstracts D

ay 1










kg-1





















P1

P2

26

Po

ster A

bstracts D

ay 1







































foodstuff

P3

P4

P5

27

Po

ster A

bstracts D

ay 1














level kit


LCMSMS
















P6

P7

28

Po

ster A

bstracts D

ay 1

EU FP7 SNIFFER (2013 - 2016)












command centre


platform















P9

P8

29

Po

ster A

bstracts D

ay 1











be compromised too



























P10

P11

30

Po

ster A

bstracts D

ay 1













































P12

P13

31

Po

ster A

bstracts D

ay 1































P14

P15

P16

32










































P17 Po

ster A

bstracts D

ay 2

P18

33

Po

ster A

bstracts D

ay 2






















food














and meat

P19

P20

34

Po

ster A

bstracts D

ay 2












presentation





























sample matrices

P21

P22

P23

35

Po

ster A

bstracts D

ay 2










































scores

P24

P25

P26

36

Po

ster A

bstracts D

ay 2












analytical matrix































P27

P28

P29

37

Po

ster A

bstracts D

ay 2















Safe operations
























P30

P31

38

List of P

articip

ants

































Denmark







39

List of P

articip

ants













Belgium



























Labolytic AS Norway


Spain

40

List of P

articip

ants










































List of P

articip

ants





























































laboratory









laboratories





43

44











Welcome from NMKL
























8

















Committee)







needs























9
















































10


























11


















method



























































in global trade











12



















13

















will be discussed





















presented including





















Honey Commission


14






























15
















drugs in foods

































non-food











16






















17
























163 participants

25 countries















18


























inspection



















as sushi as example














adapted

19


E-mail jorsdtudk











































20





















21
















and feed





































methods









22

23























































































24


































25


















Po

ster A

bstracts D

ay 1










kg-1





















P1

P2

26

Po

ster A

bstracts D

ay 1







































foodstuff

P3

P4

P5

27

Po

ster A

bstracts D

ay 1














level kit


LCMSMS
















P6

P7

28

Po

ster A

bstracts D

ay 1

EU FP7 SNIFFER (2013 - 2016)












command centre


platform















P9

P8

29

Po

ster A

bstracts D

ay 1











be compromised too



























P10

P11

30

Po

ster A

bstracts D

ay 1













































P12

P13

31

Po

ster A

bstracts D

ay 1































P14

P15

P16

32










































P17 Po

ster A

bstracts D

ay 2

P18

33

Po

ster A

bstracts D

ay 2






















food














and meat

P19

P20

34

Po

ster A

bstracts D

ay 2












presentation





























sample matrices

P21

P22

P23

35

Po

ster A

bstracts D

ay 2










































scores

P24

P25

P26

36

Po

ster A

bstracts D

ay 2












analytical matrix































P27

P28

P29

37

Po

ster A

bstracts D

ay 2















Safe operations
























P30

P31

38

List of P

articip

ants

































Denmark







39

List of P

articip

ants













Belgium



























Labolytic AS Norway


Spain

40

List of P

articip

ants










































List of P

articip

ants





























































laboratory









laboratories





43

44

















Committee)







needs























9
















































10


























11


















method



























































in global trade











12



















13

















will be discussed





















presented including





















Honey Commission


14






























15
















drugs in foods

































non-food











16






















17
























163 participants

25 countries















18


























inspection



















as sushi as example














adapted

19


E-mail jorsdtudk











































20





















21
















and feed





































methods









22

23























































































24


































25


















Po

ster A

bstracts D

ay 1










kg-1





















P1

P2

26

Po

ster A

bstracts D

ay 1







































foodstuff

P3

P4

P5

27

Po

ster A

bstracts D

ay 1














level kit


LCMSMS
















P6

P7

28

Po

ster A

bstracts D

ay 1

EU FP7 SNIFFER (2013 - 2016)












command centre


platform















P9

P8

29

Po

ster A

bstracts D

ay 1











be compromised too



























P10

P11

30

Po

ster A

bstracts D

ay 1













































P12

P13

31

Po

ster A

bstracts D

ay 1































P14

P15

P16

32










































P17 Po

ster A

bstracts D

ay 2

P18

33

Po

ster A

bstracts D

ay 2






















food














and meat

P19

P20

34

Po

ster A

bstracts D

ay 2












presentation





























sample matrices

P21

P22

P23

35

Po

ster A

bstracts D

ay 2










































scores

P24

P25

P26

36

Po

ster A

bstracts D

ay 2












analytical matrix































P27

P28

P29

37

Po

ster A

bstracts D

ay 2















Safe operations
























P30

P31

38

List of P

articip

ants

































Denmark







39

List of P

articip

ants













Belgium



























Labolytic AS Norway


Spain

40

List of P

articip

ants










































List of P

articip

ants





























































laboratory









laboratories





43

44
















































10


























11


















method



























































in global trade











12



















13

















will be discussed





















presented including





















Honey Commission


14






























15
















drugs in foods

































non-food











16






















17
























163 participants

25 countries















18


























inspection



















as sushi as example














adapted

19


E-mail jorsdtudk











































20





















21
















and feed





































methods









22

23























































































24


































25


















Po

ster A

bstracts D

ay 1










kg-1





















P1

P2

26

Po

ster A

bstracts D

ay 1







































foodstuff

P3

P4

P5

27

Po

ster A

bstracts D

ay 1














level kit


LCMSMS
















P6

P7

28

Po

ster A

bstracts D

ay 1

EU FP7 SNIFFER (2013 - 2016)












command centre


platform















P9

P8

29

Po

ster A

bstracts D

ay 1











be compromised too



























P10

P11

30

Po

ster A

bstracts D

ay 1













































P12

P13

31

Po

ster A

bstracts D

ay 1































P14

P15

P16

32










































P17 Po

ster A

bstracts D

ay 2

P18

33

Po

ster A

bstracts D

ay 2






















food














and meat

P19

P20

34

Po

ster A

bstracts D

ay 2












presentation





























sample matrices

P21

P22

P23

35

Po

ster A

bstracts D

ay 2










































scores

P24

P25

P26

36

Po

ster A

bstracts D

ay 2












analytical matrix































P27

P28

P29

37

Po

ster A

bstracts D

ay 2















Safe operations
























P30

P31

38

List of P

articip

ants

































Denmark







39

List of P

articip

ants













Belgium



























Labolytic AS Norway


Spain

40

List of P

articip

ants










































List of P

articip

ants





























































laboratory









laboratories





43

44


























11


















method



























































in global trade











12



















13

















will be discussed





















presented including





















Honey Commission


14






























15
















drugs in foods

































non-food











16






















17
























163 participants

25 countries















18


























inspection



















as sushi as example














adapted

19


E-mail jorsdtudk











































20





















21
















and feed





































methods









22

23























































































24


































25


















Po

ster A

bstracts D

ay 1










kg-1





















P1

P2

26

Po

ster A

bstracts D

ay 1







































foodstuff

P3

P4

P5

27

Po

ster A

bstracts D

ay 1














level kit


LCMSMS
















P6

P7

28

Po

ster A

bstracts D

ay 1

EU FP7 SNIFFER (2013 - 2016)












command centre


platform















P9

P8

29

Po

ster A

bstracts D

ay 1











be compromised too



























P10

P11

30

Po

ster A

bstracts D

ay 1













































P12

P13

31

Po

ster A

bstracts D

ay 1































P14

P15

P16

32










































P17 Po

ster A

bstracts D

ay 2

P18

33

Po

ster A

bstracts D

ay 2






















food














and meat

P19

P20

34

Po

ster A

bstracts D

ay 2












presentation





























sample matrices

P21

P22

P23

35

Po

ster A

bstracts D

ay 2










































scores

P24

P25

P26

36

Po

ster A

bstracts D

ay 2












analytical matrix































P27

P28

P29

37

Po

ster A

bstracts D

ay 2















Safe operations
























P30

P31

38

List of P

articip

ants

































Denmark







39

List of P

articip

ants













Belgium



























Labolytic AS Norway


Spain

40

List of P

articip

ants










































List of P

articip

ants





























































laboratory









laboratories





43

44
























































in global trade











12



















13

















will be discussed





















presented including





















Honey Commission


14






























15
















drugs in foods

































non-food











16






















17
























163 participants

25 countries















18


























inspection



















as sushi as example














adapted

19


E-mail jorsdtudk











































20





















21
















and feed





































methods









22

23























































































24


































25


















Po

ster A

bstracts D

ay 1










kg-1





















P1

P2

26

Po

ster A

bstracts D

ay 1







































foodstuff

P3

P4

P5

27

Po

ster A

bstracts D

ay 1














level kit


LCMSMS
















P6

P7

28

Po

ster A

bstracts D

ay 1

EU FP7 SNIFFER (2013 - 2016)












command centre


platform















P9

P8

29

Po

ster A

bstracts D

ay 1











be compromised too



























P10

P11

30

Po

ster A

bstracts D

ay 1













































P12

P13

31

Po

ster A

bstracts D

ay 1































P14

P15

P16

32










































P17 Po

ster A

bstracts D

ay 2

P18

33

Po

ster A

bstracts D

ay 2






















food














and meat

P19

P20

34

Po

ster A

bstracts D

ay 2












presentation





























sample matrices

P21

P22

P23

35

Po

ster A

bstracts D

ay 2










































scores

P24

P25

P26

36

Po

ster A

bstracts D

ay 2












analytical matrix































P27

P28

P29

37

Po

ster A

bstracts D

ay 2















Safe operations
























P30

P31

38

List of P

articip

ants

































Denmark







39

List of P

articip

ants













Belgium



























Labolytic AS Norway


Spain

40

List of P

articip

ants










































List of P

articip

ants





























































laboratory









laboratories





43

44



















13

















will be discussed





















presented including





















Honey Commission


14






























15
















drugs in foods

































non-food











16






















17
























163 participants

25 countries















18


























inspection



















as sushi as example














adapted

19


E-mail jorsdtudk











































20





















21
















and feed





































methods









22

23























































































24


































25


















Po

ster A

bstracts D

ay 1










kg-1





















P1

P2

26

Po

ster A

bstracts D

ay 1







































foodstuff

P3

P4

P5

27

Po

ster A

bstracts D

ay 1














level kit


LCMSMS
















P6

P7

28

Po

ster A

bstracts D

ay 1

EU FP7 SNIFFER (2013 - 2016)












command centre


platform















P9

P8

29

Po

ster A

bstracts D

ay 1











be compromised too



























P10

P11

30

Po

ster A

bstracts D

ay 1













































P12

P13

31

Po

ster A

bstracts D

ay 1































P14

P15

P16

32










































P17 Po

ster A

bstracts D

ay 2

P18

33

Po

ster A

bstracts D

ay 2






















food














and meat

P19

P20

34

Po

ster A

bstracts D

ay 2












presentation





























sample matrices

P21

P22

P23

35

Po

ster A

bstracts D

ay 2










































scores

P24

P25

P26

36

Po

ster A

bstracts D

ay 2












analytical matrix































P27

P28

P29

37

Po

ster A

bstracts D

ay 2















Safe operations
























P30

P31

38

List of P

articip

ants

































Denmark







39

List of P

articip

ants













Belgium



























Labolytic AS Norway


Spain

40

List of P

articip

ants










































List of P

articip

ants





























































laboratory









laboratories





43

44





















presented including





















Honey Commission


14






























15
















drugs in foods

































non-food











16






















17
























163 participants

25 countries















18


























inspection



















as sushi as example














adapted

19


E-mail jorsdtudk











































20





















21
















and feed





































methods









22

23























































































24


































25


















Po

ster A

bstracts D

ay 1










kg-1





















P1

P2

26

Po

ster A

bstracts D

ay 1







































foodstuff

P3

P4

P5

27

Po

ster A

bstracts D

ay 1














level kit


LCMSMS
















P6

P7

28

Po

ster A

bstracts D

ay 1

EU FP7 SNIFFER (2013 - 2016)












command centre


platform















P9

P8

29

Po

ster A

bstracts D

ay 1











be compromised too



























P10

P11

30

Po

ster A

bstracts D

ay 1













































P12

P13

31

Po

ster A

bstracts D

ay 1































P14

P15

P16

32










































P17 Po

ster A

bstracts D

ay 2

P18

33

Po

ster A

bstracts D

ay 2






















food














and meat

P19

P20

34

Po

ster A

bstracts D

ay 2












presentation





























sample matrices

P21

P22

P23

35

Po

ster A

bstracts D

ay 2










































scores

P24

P25

P26

36

Po

ster A

bstracts D

ay 2












analytical matrix































P27

P28

P29

37

Po

ster A

bstracts D

ay 2















Safe operations
























P30

P31

38

List of P

articip

ants

































Denmark







39

List of P

articip

ants













Belgium



























Labolytic AS Norway


Spain

40

List of P

articip

ants










































List of P

articip

ants





























































laboratory









laboratories





43

44






























15
















drugs in foods

































non-food











16






















17
























163 participants

25 countries















18


























inspection



















as sushi as example














adapted

19


E-mail jorsdtudk











































20





















21
















and feed





































methods









22

23























































































24


































25


















Po

ster A

bstracts D

ay 1










kg-1





















P1

P2

26

Po

ster A

bstracts D

ay 1







































foodstuff

P3

P4

P5

27

Po

ster A

bstracts D

ay 1














level kit


LCMSMS
















P6

P7

28

Po

ster A

bstracts D

ay 1

EU FP7 SNIFFER (2013 - 2016)












command centre


platform















P9

P8

29

Po

ster A

bstracts D

ay 1











be compromised too



























P10

P11

30

Po

ster A

bstracts D

ay 1













































P12

P13

31

Po

ster A

bstracts D

ay 1































P14

P15

P16

32










































P17 Po

ster A

bstracts D

ay 2

P18

33

Po

ster A

bstracts D

ay 2






















food














and meat

P19

P20

34

Po

ster A

bstracts D

ay 2












presentation





























sample matrices

P21

P22

P23

35

Po

ster A

bstracts D

ay 2










































scores

P24

P25

P26

36

Po

ster A

bstracts D

ay 2












analytical matrix































P27

P28

P29

37

Po

ster A

bstracts D

ay 2















Safe operations
























P30

P31

38

List of P

articip

ants

































Denmark







39

List of P

articip

ants













Belgium



























Labolytic AS Norway


Spain

40

List of P

articip

ants










































List of P

articip

ants





























































laboratory









laboratories





43

44

































non-food











16






















17
























163 participants

25 countries















18


























inspection



















as sushi as example














adapted

19


E-mail jorsdtudk











































20





















21
















and feed





































methods









22

23























































































24


































25


















Po

ster A

bstracts D

ay 1










kg-1





















P1

P2

26

Po

ster A

bstracts D

ay 1







































foodstuff

P3

P4

P5

27

Po

ster A

bstracts D

ay 1














level kit


LCMSMS
















P6

P7

28

Po

ster A

bstracts D

ay 1

EU FP7 SNIFFER (2013 - 2016)












command centre


platform















P9

P8

29

Po

ster A

bstracts D

ay 1











be compromised too



























P10

P11

30

Po

ster A

bstracts D

ay 1













































P12

P13

31

Po

ster A

bstracts D

ay 1































P14

P15

P16

32










































P17 Po

ster A

bstracts D

ay 2

P18

33

Po

ster A

bstracts D

ay 2






















food














and meat

P19

P20

34

Po

ster A

bstracts D

ay 2












presentation





























sample matrices

P21

P22

P23

35

Po

ster A

bstracts D

ay 2










































scores

P24

P25

P26

36

Po

ster A

bstracts D

ay 2












analytical matrix































P27

P28

P29

37

Po

ster A

bstracts D

ay 2















Safe operations
























P30

P31

38

List of P

articip

ants

































Denmark







39

List of P

articip

ants













Belgium



























Labolytic AS Norway


Spain

40

List of P

articip

ants










































List of P

articip

ants





























































laboratory









laboratories





43

44






















17
























163 participants

25 countries















18


























inspection



















as sushi as example














adapted

19


E-mail jorsdtudk











































20





















21
















and feed





































methods









22

23























































































24


































25


















Po

ster A

bstracts D

ay 1










kg-1





















P1

P2

26

Po

ster A

bstracts D

ay 1







































foodstuff

P3

P4

P5

27

Po

ster A

bstracts D

ay 1














level kit


LCMSMS
















P6

P7

28

Po

ster A

bstracts D

ay 1

EU FP7 SNIFFER (2013 - 2016)












command centre


platform















P9

P8

29

Po

ster A

bstracts D

ay 1











be compromised too



























P10

P11

30

Po

ster A

bstracts D

ay 1













































P12

P13

31

Po

ster A

bstracts D

ay 1































P14

P15

P16

32










































P17 Po

ster A

bstracts D

ay 2

P18

33

Po

ster A

bstracts D

ay 2






















food














and meat

P19

P20

34

Po

ster A

bstracts D

ay 2












presentation





























sample matrices

P21

P22

P23

35

Po

ster A

bstracts D

ay 2










































scores

P24

P25

P26

36

Po

ster A

bstracts D

ay 2












analytical matrix































P27

P28

P29

37

Po

ster A

bstracts D

ay 2















Safe operations
























P30

P31

38

List of P

articip

ants

































Denmark







39

List of P

articip

ants













Belgium



























Labolytic AS Norway


Spain

40

List of P

articip

ants










































List of P

articip

ants





























































laboratory









laboratories





43

44

163 participants

25 countries















18


























inspection



















as sushi as example














adapted

19


E-mail jorsdtudk











































20





















21
















and feed





































methods









22

23























































































24


































25


















Po

ster A

bstracts D

ay 1










kg-1





















P1

P2

26

Po

ster A

bstracts D

ay 1







































foodstuff

P3

P4

P5

27

Po

ster A

bstracts D

ay 1














level kit


LCMSMS
















P6

P7

28

Po

ster A

bstracts D

ay 1

EU FP7 SNIFFER (2013 - 2016)












command centre


platform















P9

P8

29

Po

ster A

bstracts D

ay 1











be compromised too



























P10

P11

30

Po

ster A

bstracts D

ay 1













































P12

P13

31

Po

ster A

bstracts D

ay 1































P14

P15

P16

32










































P17 Po

ster A

bstracts D

ay 2

P18

33

Po

ster A

bstracts D

ay 2






















food














and meat

P19

P20

34

Po

ster A

bstracts D

ay 2












presentation





























sample matrices

P21

P22

P23

35

Po

ster A

bstracts D

ay 2










































scores

P24

P25

P26

36

Po

ster A

bstracts D

ay 2












analytical matrix































P27

P28

P29

37

Po

ster A

bstracts D

ay 2















Safe operations
























P30

P31

38

List of P

articip

ants

































Denmark







39

List of P

articip

ants













Belgium



























Labolytic AS Norway


Spain

40

List of P

articip

ants










































List of P

articip

ants





























































laboratory









laboratories





43

44










inspection



















as sushi as example














adapted

19


E-mail jorsdtudk











































20





















21
















and feed





































methods









22

23























































































24


































25


















Po

ster A

bstracts D

ay 1










kg-1





















P1

P2

26

Po

ster A

bstracts D

ay 1







































foodstuff

P3

P4

P5

27

Po

ster A

bstracts D

ay 1














level kit


LCMSMS
















P6

P7

28

Po

ster A

bstracts D

ay 1

EU FP7 SNIFFER (2013 - 2016)












command centre


platform















P9

P8

29

Po

ster A

bstracts D

ay 1











be compromised too



























P10

P11

30

Po

ster A

bstracts D

ay 1













































P12

P13

31

Po

ster A

bstracts D

ay 1































P14

P15

P16

32










































P17 Po

ster A

bstracts D

ay 2

P18

33

Po

ster A

bstracts D

ay 2






















food














and meat

P19

P20

34

Po

ster A

bstracts D

ay 2












presentation





























sample matrices

P21

P22

P23

35

Po

ster A

bstracts D

ay 2










































scores

P24

P25

P26

36

Po

ster A

bstracts D

ay 2












analytical matrix































P27

P28

P29

37

Po

ster A

bstracts D

ay 2















Safe operations
























P30

P31

38

List of P

articip

ants

































Denmark







39

List of P

articip

ants













Belgium



























Labolytic AS Norway


Spain

40

List of P

articip

ants










































List of P

articip

ants





























































laboratory









laboratories





43

44


E-mail jorsdtudk











































20





















21
















and feed





































methods









22

23























































































24


































25


















Po

ster A

bstracts D

ay 1










kg-1





















P1

P2

26

Po

ster A

bstracts D

ay 1







































foodstuff

P3

P4

P5

27

Po

ster A

bstracts D

ay 1














level kit


LCMSMS
















P6

P7

28

Po

ster A

bstracts D

ay 1

EU FP7 SNIFFER (2013 - 2016)












command centre


platform















P9

P8

29

Po

ster A

bstracts D

ay 1











be compromised too



























P10

P11

30

Po

ster A

bstracts D

ay 1













































P12

P13

31

Po

ster A

bstracts D

ay 1































P14

P15

P16

32










































P17 Po

ster A

bstracts D

ay 2

P18

33

Po

ster A

bstracts D

ay 2






















food














and meat

P19

P20

34

Po

ster A

bstracts D

ay 2












presentation





























sample matrices

P21

P22

P23

35

Po

ster A

bstracts D

ay 2










































scores

P24

P25

P26

36

Po

ster A

bstracts D

ay 2












analytical matrix































P27

P28

P29

37

Po

ster A

bstracts D

ay 2















Safe operations
























P30

P31

38

List of P

articip

ants

































Denmark







39

List of P

articip

ants













Belgium



























Labolytic AS Norway


Spain

40

List of P

articip

ants










































List of P

articip

ants





























































laboratory









laboratories





43

44





















21
















and feed





































methods









22

23























































































24


































25


















Po

ster A

bstracts D

ay 1










kg-1





















P1

P2

26

Po

ster A

bstracts D

ay 1







































foodstuff

P3

P4

P5

27

Po

ster A

bstracts D

ay 1














level kit


LCMSMS
















P6

P7

28

Po

ster A

bstracts D

ay 1

EU FP7 SNIFFER (2013 - 2016)












command centre


platform















P9

P8

29

Po

ster A

bstracts D

ay 1











be compromised too



























P10

P11

30

Po

ster A

bstracts D

ay 1













































P12

P13

31

Po

ster A

bstracts D

ay 1































P14

P15

P16

32










































P17 Po

ster A

bstracts D

ay 2

P18

33

Po

ster A

bstracts D

ay 2






















food














and meat

P19

P20

34

Po

ster A

bstracts D

ay 2












presentation





























sample matrices

P21

P22

P23

35

Po

ster A

bstracts D

ay 2










































scores

P24

P25

P26

36

Po

ster A

bstracts D

ay 2












analytical matrix































P27

P28

P29

37

Po

ster A

bstracts D

ay 2















Safe operations
























P30

P31

38

List of P

articip

ants

































Denmark







39

List of P

articip

ants













Belgium



























Labolytic AS Norway


Spain

40

List of P

articip

ants










































List of P

articip

ants





























































laboratory









laboratories





43

44





































methods









22

23























































































24


































25


















Po

ster A

bstracts D

ay 1










kg-1





















P1

P2

26

Po

ster A

bstracts D

ay 1







































foodstuff

P3

P4

P5

27

Po

ster A

bstracts D

ay 1














level kit


LCMSMS
















P6

P7

28

Po

ster A

bstracts D

ay 1

EU FP7 SNIFFER (2013 - 2016)












command centre


platform















P9

P8

29

Po

ster A

bstracts D

ay 1











be compromised too



























P10

P11

30

Po

ster A

bstracts D

ay 1













































P12

P13

31

Po

ster A

bstracts D

ay 1































P14

P15

P16

32










































P17 Po

ster A

bstracts D

ay 2

P18

33

Po

ster A

bstracts D

ay 2






















food














and meat

P19

P20

34

Po

ster A

bstracts D

ay 2












presentation





























sample matrices

P21

P22

P23

35

Po

ster A

bstracts D

ay 2










































scores

P24

P25

P26

36

Po

ster A

bstracts D

ay 2












analytical matrix































P27

P28

P29

37

Po

ster A

bstracts D

ay 2















Safe operations
























P30

P31

38

List of P

articip

ants

































Denmark







39

List of P

articip

ants













Belgium



























Labolytic AS Norway


Spain

40

List of P

articip

ants










































List of P

articip

ants





























































laboratory









laboratories





43

44

23























































































24


































25


















Po

ster A

bstracts D

ay 1










kg-1





















P1

P2

26

Po

ster A

bstracts D

ay 1







































foodstuff

P3

P4

P5

27

Po

ster A

bstracts D

ay 1














level kit


LCMSMS
















P6

P7

28

Po

ster A

bstracts D

ay 1

EU FP7 SNIFFER (2013 - 2016)












command centre


platform















P9

P8

29

Po

ster A

bstracts D

ay 1











be compromised too



























P10

P11

30

Po

ster A

bstracts D

ay 1













































P12

P13

31

Po

ster A

bstracts D

ay 1































P14

P15

P16

32










































P17 Po

ster A

bstracts D

ay 2

P18

33

Po

ster A

bstracts D

ay 2






















food














and meat

P19

P20

34

Po

ster A

bstracts D

ay 2












presentation





























sample matrices

P21

P22

P23

35

Po

ster A

bstracts D

ay 2










































scores

P24

P25

P26

36

Po

ster A

bstracts D

ay 2












analytical matrix































P27

P28

P29

37

Po

ster A

bstracts D

ay 2















Safe operations
























P30

P31

38

List of P

articip

ants

































Denmark







39

List of P

articip

ants













Belgium



























Labolytic AS Norway


Spain

40

List of P

articip

ants










































List of P

articip

ants





























































laboratory









laboratories





43

44










































24


































25


















Po

ster A

bstracts D

ay 1










kg-1





















P1

P2

26

Po

ster A

bstracts D

ay 1







































foodstuff

P3

P4

P5

27

Po

ster A

bstracts D

ay 1














level kit


LCMSMS
















P6

P7

28

Po

ster A

bstracts D

ay 1

EU FP7 SNIFFER (2013 - 2016)












command centre


platform















P9

P8

29

Po

ster A

bstracts D

ay 1











be compromised too



























P10

P11

30

Po

ster A

bstracts D

ay 1













































P12

P13

31

Po

ster A

bstracts D

ay 1































P14

P15

P16

32










































P17 Po

ster A

bstracts D

ay 2

P18

33

Po

ster A

bstracts D

ay 2






















food














and meat

P19

P20

34

Po

ster A

bstracts D

ay 2












presentation





























sample matrices

P21

P22

P23

35

Po

ster A

bstracts D

ay 2










































scores

P24

P25

P26

36

Po

ster A

bstracts D

ay 2












analytical matrix































P27

P28

P29

37

Po

ster A

bstracts D

ay 2















Safe operations
























P30

P31

38

List of P

articip

ants

































Denmark







39

List of P

articip

ants













Belgium



























Labolytic AS Norway


Spain

40

List of P

articip

ants










































List of P

articip

ants





























































laboratory









laboratories





43

44


































25


















Po

ster A

bstracts D

ay 1










kg-1





















P1

P2

26

Po

ster A

bstracts D

ay 1







































foodstuff

P3

P4

P5

27

Po

ster A

bstracts D

ay 1














level kit


LCMSMS
















P6

P7

28

Po

ster A

bstracts D

ay 1

EU FP7 SNIFFER (2013 - 2016)












command centre


platform















P9

P8

29

Po

ster A

bstracts D

ay 1











be compromised too



























P10

P11

30

Po

ster A

bstracts D

ay 1













































P12

P13

31

Po

ster A

bstracts D

ay 1































P14

P15

P16

32










































P17 Po

ster A

bstracts D

ay 2

P18

33

Po

ster A

bstracts D

ay 2






















food














and meat

P19

P20

34

Po

ster A

bstracts D

ay 2












presentation





























sample matrices

P21

P22

P23

35

Po

ster A

bstracts D

ay 2










































scores

P24

P25

P26

36

Po

ster A

bstracts D

ay 2












analytical matrix































P27

P28

P29

37

Po

ster A

bstracts D

ay 2















Safe operations
























P30

P31

38

List of P

articip

ants

































Denmark







39

List of P

articip

ants













Belgium



























Labolytic AS Norway


Spain

40

List of P

articip

ants










































List of P

articip

ants





























































laboratory









laboratories





43

44

Po

ster A

bstracts D

ay 1










kg-1





















P1

P2

26

Po

ster A

bstracts D

ay 1







































foodstuff

P3

P4

P5

27

Po

ster A

bstracts D

ay 1














level kit


LCMSMS
















P6

P7

28

Po

ster A

bstracts D

ay 1

EU FP7 SNIFFER (2013 - 2016)












command centre


platform















P9

P8

29

Po

ster A

bstracts D

ay 1











be compromised too



























P10

P11

30

Po

ster A

bstracts D

ay 1













































P12

P13

31

Po

ster A

bstracts D

ay 1































P14

P15

P16

32










































P17 Po

ster A

bstracts D

ay 2

P18

33

Po

ster A

bstracts D

ay 2






















food














and meat

P19

P20

34

Po

ster A

bstracts D

ay 2












presentation





























sample matrices

P21

P22

P23

35

Po

ster A

bstracts D

ay 2










































scores

P24

P25

P26

36

Po

ster A

bstracts D

ay 2












analytical matrix































P27

P28

P29

37

Po

ster A

bstracts D

ay 2















Safe operations
























P30

P31

38

List of P

articip

ants

































Denmark







39

List of P

articip

ants













Belgium



























Labolytic AS Norway


Spain

40

List of P

articip

ants










































List of P

articip

ants





























































laboratory









laboratories





43

44

Po

ster A

bstracts D

ay 1







































foodstuff

P3

P4

P5

27

Po

ster A

bstracts D

ay 1














level kit


LCMSMS
















P6

P7

28

Po

ster A

bstracts D

ay 1

EU FP7 SNIFFER (2013 - 2016)












command centre


platform















P9

P8

29

Po

ster A

bstracts D

ay 1











be compromised too



























P10

P11

30

Po

ster A

bstracts D

ay 1













































P12

P13

31

Po

ster A

bstracts D

ay 1































P14

P15

P16

32










































P17 Po

ster A

bstracts D

ay 2

P18

33

Po

ster A

bstracts D

ay 2






















food














and meat

P19

P20

34

Po

ster A

bstracts D

ay 2












presentation





























sample matrices

P21

P22

P23

35

Po

ster A

bstracts D

ay 2










































scores

P24

P25

P26

36

Po

ster A

bstracts D

ay 2












analytical matrix































P27

P28

P29

37

Po

ster A

bstracts D

ay 2















Safe operations
























P30

P31

38

List of P

articip

ants

































Denmark







39

List of P

articip

ants













Belgium



























Labolytic AS Norway


Spain

40

List of P

articip

ants










































List of P

articip

ants





























































laboratory









laboratories





43

44

Po

ster A

bstracts D

ay 1














level kit


LCMSMS
















P6

P7

28

Po

ster A

bstracts D

ay 1

EU FP7 SNIFFER (2013 - 2016)












command centre


platform















P9

P8

29

Po

ster A

bstracts D

ay 1











be compromised too



























P10

P11

30

Po

ster A

bstracts D

ay 1













































P12

P13

31

Po

ster A

bstracts D

ay 1































P14

P15

P16

32










































P17 Po

ster A

bstracts D

ay 2

P18

33

Po

ster A

bstracts D

ay 2






















food














and meat

P19

P20

34

Po

ster A

bstracts D

ay 2












presentation





























sample matrices

P21

P22

P23

35

Po

ster A

bstracts D

ay 2










































scores

P24

P25

P26

36

Po

ster A

bstracts D

ay 2












analytical matrix































P27

P28

P29

37

Po

ster A

bstracts D

ay 2















Safe operations
























P30

P31

38

List of P

articip

ants

































Denmark







39

List of P

articip

ants













Belgium



























Labolytic AS Norway


Spain

40

List of P

articip

ants










































List of P

articip

ants





























































laboratory









laboratories





43

44

Po

ster A

bstracts D

ay 1

EU FP7 SNIFFER (2013 - 2016)












command centre


platform















P9

P8

29

Po

ster A

bstracts D

ay 1











be compromised too



























P10

P11

30

Po

ster A

bstracts D

ay 1













































P12

P13

31

Po

ster A

bstracts D

ay 1































P14

P15

P16

32










































P17 Po

ster A

bstracts D

ay 2

P18

33

Po

ster A

bstracts D

ay 2






















food














and meat

P19

P20

34

Po

ster A

bstracts D

ay 2












presentation





























sample matrices

P21

P22

P23

35

Po

ster A

bstracts D

ay 2










































scores

P24

P25

P26

36

Po

ster A

bstracts D

ay 2












analytical matrix































P27

P28

P29

37

Po

ster A

bstracts D

ay 2















Safe operations
























P30

P31

38

List of P

articip

ants

































Denmark







39

List of P

articip

ants













Belgium



























Labolytic AS Norway


Spain

40

List of P

articip

ants










































List of P

articip

ants





























































laboratory









laboratories





43

44

Po

ster A

bstracts D

ay 1











be compromised too



























P10

P11

30

Po

ster A

bstracts D

ay 1













































P12

P13

31

Po

ster A

bstracts D

ay 1































P14

P15

P16

32










































P17 Po

ster A

bstracts D

ay 2

P18

33

Po

ster A

bstracts D

ay 2






















food














and meat

P19

P20

34

Po

ster A

bstracts D

ay 2












presentation





























sample matrices

P21

P22

P23

35

Po

ster A

bstracts D

ay 2










































scores

P24

P25

P26

36

Po

ster A

bstracts D

ay 2












analytical matrix































P27

P28

P29

37

Po

ster A

bstracts D

ay 2















Safe operations
























P30

P31

38

List of P

articip

ants

































Denmark







39

List of P

articip

ants













Belgium



























Labolytic AS Norway


Spain

40

List of P

articip

ants










































List of P

articip

ants





























































laboratory









laboratories





43

44

Po

ster A

bstracts D

ay 1













































P12

P13

31

Po

ster A

bstracts D

ay 1































P14

P15

P16

32










































P17 Po

ster A

bstracts D

ay 2

P18

33

Po

ster A

bstracts D

ay 2






















food














and meat

P19

P20

34

Po

ster A

bstracts D

ay 2












presentation





























sample matrices

P21

P22

P23

35

Po

ster A

bstracts D

ay 2










































scores

P24

P25

P26

36

Po

ster A

bstracts D

ay 2












analytical matrix































P27

P28

P29

37

Po

ster A

bstracts D

ay 2















Safe operations
























P30

P31

38

List of P

articip

ants

































Denmark







39

List of P

articip

ants













Belgium



























Labolytic AS Norway


Spain

40

List of P

articip

ants










































List of P

articip

ants





























































laboratory









laboratories





43

44

Po

ster A

bstracts D

ay 1































P14

P15

P16

32










































P17 Po

ster A

bstracts D

ay 2

P18

33

Po

ster A

bstracts D

ay 2






















food














and meat

P19

P20

34

Po

ster A

bstracts D

ay 2












presentation





























sample matrices

P21

P22

P23

35

Po

ster A

bstracts D

ay 2










































scores

P24

P25

P26

36

Po

ster A

bstracts D

ay 2












analytical matrix































P27

P28

P29

37

Po

ster A

bstracts D

ay 2















Safe operations
























P30

P31

38

List of P

articip

ants

































Denmark







39

List of P

articip

ants













Belgium



























Labolytic AS Norway


Spain

40

List of P

articip

ants










































List of P

articip

ants





























































laboratory









laboratories





43

44










































P17 Po

ster A

bstracts D

ay 2

P18

33

Po

ster A

bstracts D

ay 2






















food














and meat

P19

P20

34

Po

ster A

bstracts D

ay 2












presentation





























sample matrices

P21

P22

P23

35

Po

ster A

bstracts D

ay 2










































scores

P24

P25

P26

36

Po

ster A

bstracts D

ay 2












analytical matrix































P27

P28

P29

37

Po

ster A

bstracts D

ay 2















Safe operations
























P30

P31

38

List of P

articip

ants

































Denmark







39

List of P

articip

ants













Belgium



























Labolytic AS Norway


Spain

40

List of P

articip

ants










































List of P

articip

ants





























































laboratory









laboratories





43

44

Po

ster A

bstracts D

ay 2






















food














and meat

P19

P20

34

Po

ster A

bstracts D

ay 2












presentation





























sample matrices

P21

P22

P23

35

Po

ster A

bstracts D

ay 2










































scores

P24

P25

P26

36

Po

ster A

bstracts D

ay 2












analytical matrix































P27

P28

P29

37

Po

ster A

bstracts D

ay 2















Safe operations
























P30

P31

38

List of P

articip

ants

































Denmark







39

List of P

articip

ants













Belgium



























Labolytic AS Norway


Spain

40

List of P

articip

ants










































List of P

articip

ants





























































laboratory









laboratories





43

44

Po

ster A

bstracts D

ay 2












presentation





























sample matrices

P21

P22

P23

35

Po

ster A

bstracts D

ay 2










































scores

P24

P25

P26

36

Po

ster A

bstracts D

ay 2












analytical matrix































P27

P28

P29

37

Po

ster A

bstracts D

ay 2















Safe operations
























P30

P31

38

List of P

articip

ants

































Denmark







39

List of P

articip

ants













Belgium



























Labolytic AS Norway


Spain

40

List of P

articip

ants










































List of P

articip

ants





























































laboratory









laboratories





43

44

Po

ster A

bstracts D

ay 2










































scores

P24

P25

P26

36

Po

ster A

bstracts D

ay 2












analytical matrix































P27

P28

P29

37

Po

ster A

bstracts D

ay 2















Safe operations
























P30

P31

38

List of P

articip

ants

































Denmark







39

List of P

articip

ants













Belgium



























Labolytic AS Norway


Spain

40

List of P

articip

ants










































List of P

articip

ants





























































laboratory









laboratories





43

44

Po

ster A

bstracts D

ay 2












analytical matrix































P27

P28

P29

37

Po

ster A

bstracts D

ay 2















Safe operations
























P30

P31

38

List of P

articip

ants

































Denmark







39

List of P

articip

ants













Belgium



























Labolytic AS Norway


Spain

40

List of P

articip

ants










































List of P

articip

ants





























































laboratory









laboratories





43

44

Po

ster A

bstracts D

ay 2















Safe operations
























P30

P31

38

List of P

articip

ants

































Denmark







39

List of P

articip

ants













Belgium



























Labolytic AS Norway


Spain

40

List of P

articip

ants










































List of P

articip

ants





























































laboratory









laboratories





43

44

List of P

articip

ants

































Denmark







39

List of P

articip

ants













Belgium



























Labolytic AS Norway


Spain

40

List of P

articip

ants










































List of P

articip

ants





























































laboratory









laboratories





43

44

List of P

articip

ants













Belgium



























Labolytic AS Norway


Spain

40

List of P

articip

ants










































List of P

articip

ants





























































laboratory









laboratories





43

44

List of P

articip

ants










































List of P

articip

ants





























































laboratory









laboratories





43

44

List of P

articip

ants





























































laboratory









laboratories





43

44



















laboratory









laboratories





43

44

44

Documents

ood OOD ABS A CRYSTAL BALL LabsLabs - AOAC Europe … · Dr. Eric Konings, President of AOAC International, Nestle, Switzerland í ò: ì - í ó: ì EU policy on contaminants in