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154 Poster presentations P306 CALCIUM, PHOSPHORUS AND MAGNESIUM BIOMARKERS IN PRIMARY TEETH OF PREMATURELY BORN CHILDREN E. Planells 1 , E.E. Moreno 2 , D. Florea 1 , M. García ´ Avila 1 , M. Moreno Gald´ o 3 ,E.Mill´an 1 , J. Salmer´ on 4 , P. Planells 5 . 1 Physiology Dept, University of Granada, Granada, 2 Odontopediatry Dpt, University Complutense, Madrid, 3 Pediatrry Unit, Maternal Hospital, Granada, 4 Quirurgic Dpt, 5 Odonthopaediatry Dpt, University Complutense, Madrid, Spain Rationale: Recent studies have demonstrated that chil- dren born prematurely (BW < 1.5 kg) have lower bone mineral content and density compared with children born at term gestation. The objective is to study the dental mineralization of children born prematurely (PT) by analyzing calcium, phosphorus and magnesium as biomarkers of nutritional status in these elements comparing with a control group of children born at term (AT). Methods: A cohort of 30 children (6 8 y) PT group was selected and primary dental mineral status was compared with these of 25 AT healthy children (6 9 y). A food intake questionnaire (Mataix, 2007 software) was asked to obtain %RDA intakes of calcium, phosphorus and magnesium. Calcium and magnesium content of wet mineralized samples were determined by flame atomic absorption spectrometry, phosphorus was analyzed in same samples by colorimetric Fiske Subbarow method. Results: No significant differences were obtained in calcium, phosphorus and magnesium intakes between groups. PT children had lower dental calcium, phosphorus and magnesium content than AT children: 188.5±10.2. vs, 254.3±12.3, 100.4±5.6 vs 138.8±9.2 and 5.34±1.5 vs 6.74±1.3 (mg/g dry theeth), respectively (p < 0.05). No significant differences were found in phosphorus content between two groups. Conclusion: PT group of children have lower primary teeth calcium and magnesium content, compared with AT children. Mineral supplementation of the mother during pregnancy an/or of the early diet of PT infant may be needed, taking into account that genetic determinants may influence on bone mineralization of PT infants. Disclosure of Interest: I declare that I do not have any affiliation with or financial interest in a commercial organisation that poses a conflict of interest. P307 ASSESSMENT OF NUTRITIONAL STATUS AND ANEMIA IN INFANTS AND PRE-SCHOOL OF A DAY CARE IN ONE CITY FROM SOUTH OF THE COUNTRY J.L. Cardoso 1 , C.C.G.M. Moreira 2 , M.M.F.L. Lazzarotto 2 , G.G.K.B. Bonafim 2 . 1 School Health, Municipal Health Secretariat, 2 Pediatrics, Federal University of Santa Catarina, Florian´ opolis, Brazil Rationale: To acess nutritional status, anemia frequency and the association of these with family income, in enrolled in a day care in one city from south of the country. Methods: It is a cross-sectional study accomplished in 73 children, from one to six years old; anthropometric assessment and laboratorial determination of hemoglobin was carried out: nutritional status determined according to curves of OMS, according weight for stature and mass index corporal for age in Z-score; it was considered anemia when hemoglobin below 11 g/dl; the family income was appraised as per capita income in minimum wages. Results: About wheight for stature in Z-score, 91.78% of the children were classified as eutrophic, 1.37% as low wheight for stature and 6.85% as high wheight for stature. According index of corporal mass for age, 67.12% had appropriate index; 1.37% had low index; 23.29% had overwheight and 8.22% obesity. Anemia prevalence was 8.22%. In eutrophic children, 6.12% were anemic; in overwheight children, 17.65% were anemic. There were not cases of anemia in undernourished or obese children. Overweight or obesity were found in 32% of the children with family income per captures smaller than half minimum wage and in 30% in the others. Of the children with anemia, 100% have family income less than half minimum wage per capita. Conclusion: Anemia frequency in this sample is low and it has association with low income per capita. The main nutritional disorders found were overweight and obesity, and nutritional status doesn’t have association with the family income. Overweight children have higher frequency of anemia. References Coutinho GGPL. Iron deficiency anemia in children: a challeng for public helth and for society. Med. J. v.132.n2.p.66 73, ao Paulo, 2001. Disclosure of Interest: None declared. Paediatrics II P308 EARLY ENTERAL FEEDING THROUGH NEEDLE CATHETER JEJUNOSTOMY IN SURGICAL NEONATES J. Wu 1 , W. Cai 1 . 1 Clinical Nutrition, Xin Hua Hospital affiliated to Shanghai Jiaotong University School of Medicine, Shanghai, China Rationale: To evaluate post-operative jejunostomy feed- ing in terms of nutritional, gastrointestinal and mechani- cal complications in neonates after upper gastrointestinal surgery. Methods: We retrospectively collected medical records of surgical neonates with needle catheter jejunostomy (NCJ) performed during upper gastrointestinal operation from Jan, 2003 to Dec, 2008. Details of patient characteristics, including gestational age, birthweight, age at operation, pre- and post-operative body weight, malformation char- acteristics, surgical procedures, parenteral and enteral nutrition use, complications, and length of hospital stay were obtained. Results: A total of 38 neonates (14 girl/24 boy, 13 preterm/25 full term)) with a mean birthweight of 2835 g (range 1155 to 3950) were enrolled. The average age at operation was 6 days (range 1 to 28) with a

P307 ASSESSMENT OF NUTRITIONAL STATUS AND ANEMIA IN INFANTS AND PRE-SCHOOL OF A DAY CARE IN ONE CITY FROM SOUTH OF THE COUNTRY

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154 Poster presentations

P306CALCIUM, PHOSPHORUS AND MAGNESIUM BIOMARKERSIN PRIMARY TEETH OF PREMATURELY BORN CHILDREN

E. Planells1, E.E. Moreno2, D. Florea1, M. García Avila1,M. Moreno Galdo3, E. Millan1, J. Salmeron4, P. Planells5.1Physiology Dept, University of Granada, Granada,2Odontopediatry Dpt, University Complutense, Madrid,3Pediatrry Unit, Maternal Hospital, Granada, 4QuirurgicDpt, 5Odonthopaediatry Dpt, University Complutense,Madrid, Spain

Rationale: Recent studies have demonstrated that chil-dren born prematurely (BW < 1.5 kg) have lower bonemineral content and density compared with childrenborn at term gestation. The objective is to studythe dental mineralization of children born prematurely(PT) by analyzing calcium, phosphorus and magnesiumas biomarkers of nutritional status in these elementscomparing with a control group of children born at term(AT).Methods: A cohort of 30 children (6 8 y) PT group wasselected and primary dental mineral status was comparedwith these of 25 AT healthy children (6 9 y). A foodintake questionnaire (Mataix, 2007 software) was askedto obtain %RDA intakes of calcium, phosphorus andmagnesium. Calcium and magnesium content of wetmineralized samples were determined by flame atomicabsorption spectrometry, phosphorus was analyzed insame samples by colorimetric Fiske Subbarow method.Results: No significant differences were obtained incalcium, phosphorus and magnesium intakes betweengroups. PT children had lower dental calcium, phosphorusand magnesium content than AT children: 188.5±10.2.vs, 254.3±12.3, 100.4±5.6 vs 138.8±9.2 and 5.34±1.5 vs6.74±1.3 (mg/g dry theeth), respectively (p < 0.05). Nosignificant differences were found in phosphorus contentbetween two groups.Conclusion: PT group of children have lower primaryteeth calcium and magnesium content, compared with ATchildren. Mineral supplementation of the mother duringpregnancy an/or of the early diet of PT infant may beneeded, taking into account that genetic determinantsmay influence on bone mineralization of PT infants.

Disclosure of Interest: I declare that I do not have any affiliationwith or financial interest in a commercial organisation that posesa conflict of interest.

P307ASSESSMENT OF NUTRITIONAL STATUS AND ANEMIA ININFANTS AND PRE-SCHOOL OF A DAY CARE IN ONE CITYFROM SOUTH OF THE COUNTRYJ.L. Cardoso1, C.C.G.M. Moreira2, M.M.F.L. Lazzarotto2,G.G.K.B. Bonafim2. 1School Health, Municipal HealthSecretariat, 2Pediatrics, Federal University of SantaCatarina, Florianopolis, Brazil

Rationale: To acess nutritional status, anemia frequencyand the association of these with family income, inenrolled in a day care in one city from south of thecountry.Methods: It is a cross-sectional study accomplished in73 children, from one to six years old; anthropometric

assessment and laboratorial determination of hemoglobinwas carried out: nutritional status determined accordingto curves of OMS, according weight for stature and massindex corporal for age in Z-score; it was consideredanemia when hemoglobin below 11 g/dl; the familyincome was appraised as per capita income in minimumwages.Results: About wheight for stature in Z-score, 91.78%of the children were classified as eutrophic, 1.37% aslow wheight for stature and 6.85% as high wheight forstature. According index of corporal mass for age, 67.12%had appropriate index; 1.37% had low index; 23.29%had overwheight and 8.22% obesity. Anemia prevalencewas 8.22%. In eutrophic children, 6.12% were anemic;in overwheight children, 17.65% were anemic. Therewere not cases of anemia in undernourished or obesechildren. Overweight or obesity were found in 32% of thechildren with family income per captures smaller thanhalf minimum wage and in 30% in the others. Of thechildren with anemia, 100% have family income less thanhalf minimum wage per capita.Conclusion: Anemia frequency in this sample is lowand it has association with low income per capita. Themain nutritional disorders found were overweight andobesity, and nutritional status doesn’t have associationwith the family income. Overweight children have higherfrequency of anemia.

ReferencesCoutinho GGPL. Iron deficiency anemia in children: a challeng

for public helth and for society. Med. J. v.132.n2.p.66 73,Sao Paulo, 2001.

Disclosure of Interest: None declared.

Paediatrics II

P308EARLY ENTERAL FEEDING THROUGH NEEDLE CATHETERJEJUNOSTOMY IN SURGICAL NEONATESJ. Wu1, W. Cai1. 1Clinical Nutrition, Xin Hua Hospitalaffiliated to Shanghai Jiaotong University School ofMedicine, Shanghai, China

Rationale: To evaluate post-operative jejunostomy feed-ing in terms of nutritional, gastrointestinal and mechani-cal complications in neonates after upper gastrointestinalsurgery.Methods: We retrospectively collected medical records ofsurgical neonates with needle catheter jejunostomy (NCJ)performed during upper gastrointestinal operation fromJan, 2003 to Dec, 2008. Details of patient characteristics,including gestational age, birthweight, age at operation,pre- and post-operative body weight, malformation char-acteristics, surgical procedures, parenteral and enteralnutrition use, complications, and length of hospital staywere obtained.Results: A total of 38 neonates (14 girl/24 boy,13 preterm/25 full term)) with a mean birthweight of2835 g (range 1155 to 3950) were enrolled. The averageage at operation was 6 days (range 1 to 28) with a