PCR F P C RFA M I LY & -4 AMPLITUBE™ PCR REACTION TUBES, 0.5 ml Made of polypropylene Also designed for oil-free opera it o n , the inside of these tubes has a polished s u r f a

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Sometimes it’s the small things that count.The PCRackTM will accept all models of 0.2 ml tubes, along with strips of 8 or 12t u b e s . P l ates of 96 wells can also be accommodat e d . The PCRackT M can behorizontally attached to each other in order to build-up any configuration youdesire.

The assurance of highly accurate and contaminant-freeproceduresDriven by innovat i o n , research and product development,S i m p o r t is a world leading laborat o ry products designand manufacturing company. Since 1975, S i m p o r t h a sd e v e l o p e d , manufactured and marketed a broad range ofi n n o v ative disposables to improve research techniquesand methods. Our products are distributed worldwidethrough reputable labora t o r y and medical p roductsd i s t r i b u t o r s . Some of our superior quality products areal so d is t r ibu ted under p r iva te labe l by some of thew o r l d ’s leading laborat o ry products manufacturers ands u p p l i e r s .

A l l S i m p o r t P C R ® p ro duc t s a re d es i gne d andmanufactured to the highest qual ity standards and toprec ise cal ibrat ion and dimensional accuracy. M a d eunder the most r ig id manu factur ing condit ions . T h eS i m p o r t P C R ® Fami l y w as de v e l ope d t o he lp t her e s e a r c h e r, analyst and technician obtain accurate andr e p e atable results from experimenta t i o n , test ing anda n a l y s i s .

PCR tubes, strips and plates are also available sterile onspecial request. When placing your order, please checkwith Customer Service to find out minimum quantitiesand expected delivery.

The Simport Plus Fa c t o rManufac tur ing to the highest qual i t y s tandards andprecision does not alone qual ify an item to make it aS i m p o r t p r o d u c t . I n d e e d , i n n o v ation must be brought inas “The Plus Fa c t o r ” .

T H E P C R®

FA M I LY

PCR® FAMILY

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1 Integral shield prevents contamination with surface of lid

2 Domed cap provides a snap shut positive seal

3 Pierceable flat cap

4 Attached cap allows opening and closing with one hand

5 Frosted writing surface

6 Ultrathin wall ensures excellent thermal exchange

7 Round bottom makes tube easy to empty

Cat. # Cat. #Domed Cap Flat Cap Color Qty/PkT325-1N T325-2N Natural 1000T325-1B T325-2B Blue 1000T325-1G T325-2G Green 1000T325-1R T325-2R Red 1000T325-1Y T325-2Y Yellow 1000

T325-1 & -2 AMPLITUBE™

PCR REACTION TUBES, 0.2 mlMade of polypropylene

Designed for oil-free operat i o n , these tubes are made of transparentsuperior quality grade polypropylene for better viewing of the contents.Their ultrathin wall design will ensure rapid thermal transfer and asignificant reduction in cy cle and PCR reaction time.

Attached hinged caps are either dome or flat-topped and can be used withh e ated lids used by thermal cy cler manufacturers. They provide positivesealing during thermal cy cling and will prevent evap o r ation while beingeasily opened and closed with one hand. The cap has an integral shieldpreventing contamination with surface of lid. Frosted writing surfacefor sample identificat i o n .

Choice of colorless and four non-cytotoxic and non-metallic colors.Pa c k aged in tamperproof resealable safety-lock bag s . A u t o cl av a b l e .

Cat. # Cat. #Domed Cap Flat Cap Color Qty/PkT325-1VN T325-2VN Natural 1000T325-1VB T325-2VB Blue 1000T325-1VG T325-2VG Green 1000T325-1VR T325-2VR Red 1000T325-1VY T325-2VY Yellow 1000

T325-1V & -2V AMPLITUBE™


Ideal tube design when centrifugation is necessary. These tubes are identical tothe T325-1 & -2 Series but without a contamination shield. Frosted writingsurface for sample identificat i o n . See description above for further details.Packaged in tamperproof resealable safety-lock bags.

Series T325-1VDomed cap model

Series T325-2VFlat cap model

All products on this page are certified

RNase, DNaseand Pyrogen safe.

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T325-1Domed Cap Series

T325-2Flat Cap Series

The cap has anintegral shieldpreventingcontaminationwith surface of lid.

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T325-3 & -4AMPLITUBE™


Also designed for oil-free operat i o n , the inside of these tubes has a polisheds u r f a c e , a conical shape and a round bottom for maximum sample recovery.They offer optimum contact with thermal cy cler blocks. Their ultrathin walldesign will ensure rapid thermal transfer and a significant reduction in cy cl eand PCR reaction time. G r a d u ated in 0.1 ml increments.

Attached hinged caps are either dome or flat-topped and provide positivesealing during thermal cy cling stag e s . They will prevent evap o r ation whilebeing easily opened and closed with one hand. Choice of colorless andfour non-cytotoxic and non-metallic colors for visual coding of samples.Packed in tamperproof resealable bag s . A u t o cl av a b l e .

The Combi-RackT M can hold up to96 0.2 ml PCR tubes and 8- or 12-tube strips.

Three versatile racks to accommodate your PCR tubes, strips and plates.

1 Domed cap provides a snap shut positive seal2 Pierceable flat cap3 Attached cap allows opening and closing with one hand4 Etched writing surface for sample identification5 Graduated in 0.1 ml increments6 Ultrathin wall7 See-thru polypropylene8 Round bottom makes tube easy to empty

T325-3Domed Cap Series

T325-4Flat Cap Series

The UnirackT M is an almost universalsupport holding up to 60 PCR reactiontubes between 0.2 ml and 0.5 mlcapacity.

Cat. # Cat. #Domed Cap Flat Cap Color Qty/PkT325-3N T325-4N Natural 1000T325-3B T325-4B Blue 1000T325-3G T325-4G Green 1000T325-3R T325-4R Red 1000T325-3Y T325-4Y Yellow 1000

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The PCRackT M will accept allmodels of 0.2 ml tubes, along withstrips of 8 or 12 tubes and 96-wellplates.

T325-12AMPLITUBE™

PCR REACTION TUBES (without cap)Made of polypropylene

This thin wall 0.2 ml tube is very useful when processing smaller volumes.It offers optimum contact with thermal cy cler blocks. The ultrathin wall willensure rapid thermal transfer and a significant reduction in cy cle and PCRreaction time. Specially designed with a highly polished surface and around bottom for maximum sample recovery. Sealing can be achieved byusing either T321-1 or T321-2 Series Cap Strips. Choice of colorless andfour non-cytotoxic and non-metallic colors. Packed in tamperproofresealable bag s .

Cat. # Color Qty/PkT325-12N Natural 1000T325-12B Blue 1000T325-12G Green 1000T325-12R Red 1000T325-12Y Yellow 1000

See page 77See page 77See page 102

All products on thispage are certifiedRNase, DNase and

Pyrogen safe.

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Made of polypropylene

S i m p o r t Reaction Strips include 8 or 12 integral 0.2 ml tubes with ultrathin sidewalls andbottoms for more uniform and efficient temperature transfer, therefore reducing PCR reactiontime in most 96-well “ V ” bottomed thermal cy clers such as MJ Research, Perkin Elmer, H y b a i dand others.

They are more easily handled than single tubes. They will precisely fit standard well spacingand can also be used with 8- and 12-channel hand-held pipettors. All strips are molded ofpolypropylene under the most stringent condi t ions and a re offe red, co lor less and in fourdifferent colors.

Non-attached cap strips are available in a dome or flat top design and ensure a perfectclosure during the whole thermal cy cl e . C ap strips are not included and have to be ordereds e p a r ately (see T321 Series). A u t o cl av a b l e . Packed in tamperproof resealable bag s .

1 Pierceable flat cap

2 Domed cap also provides a snap shut positive seal

3 Can be cut and used as individual tubes

4 Ultrathin wall

5 Standard well spacing

6 No carry-over contamination

TUBE & CAP STRIPS OF 8Tubes Strip Domed Cap Flat Cap Color Qty/PkT320-1N T321-1N T321-2N Natural 125T320-1B T321-1B T321-2B Blue 125T320-1G T321-1G T321-2G Green 125T320-1R T321-1R T321-2R Red 125T320-1Y T321-1Y T321-2Y Yellow 125

TUBE & CAP STRIPS OF 12Tubes Strip Domed Cap Color Qty/PkT320-10N T321-3N Natural 125T320-10B — Blue 125T320-10G — Green 125T320-10R — Red 125T320-10Y — Yellow 125

T321-2 FlatCap Strip

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T320-1 Tube Strip

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T321-1 DomeCap Strip

This PCR Reaction Strip can

be cut to any length.

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T321-3N

T320-10

T321-2

T320-1

T321-1

All products on this page are certifiedRNase, DNase and Pyrogen safe.

T320AMPLITUBE™

PCR REACTION STRIPSMade of polypropylene

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This more convenient 0.2 ml tube strip incorporates individually attached cap s . No need to carry two separate components in inventory.

The strip includes 8 integral 0.2 ml tubes with ultrathin sidewalls and bottoms for more uniform and efficient temperature transfer.

This PCR reaction strip is available with either flat (needle pierceable) or dome-topped individually attached hinged cap s . While easilyopened and closed with one hand, their positive sealing will fully protect the contents from evap o r ation during the whole thermal cy cl e . T h ec ap has an integral seal preventing contamination with surface of lid.

Wh i le more eas i l y han d l ed th an s ing l e tu bes , t h e st r i p wi l l p r ec i se l y f i t s t and a rd w e l l s pac in g an d c an a ls o b e u sed w i th8-channel hand-held pipettors. Manufactured under the most stringent conditions to attain the highest quality standards in the industry.Choice of colorless and four non-cytotoxic and non-metallic colors. A u t o cl av a b l e . Packed in tamperproof resealable bag s .

T322AMPLITUBE™THIN WALL PCR REACTION STRIPS (Cap Strip attached)Made of polypropylene

Designed for oil-free operat i o n , these reaction strips are identical to T 3 2 0Series but include already attached 8-cap strips molded with a living hinge tof a c i l i t ate opening and cl o s i n g . They are manufactured under strict qualitycontrol supervision to ensure reproducible results, using a special almosttransparent polypropylene.

Individual tube sealing ensures that samples are well protected from any carry-over contaminat i o n . C ap design offers a snap shut seal to avoid evap o r at i o nduring thermal cycling stages. Choice of colorless and four non-cytotoxic andnon-metallic colors for visual coding of samples. A u t o cl av a b l e . Packed intamperproof resealable bags.

Cat. # Color Qty/PkT322-1N Natural 125T322-1B Blue 125T322-1G Green 125T322-1R Red 125T322-1Y Yellow 125

Cat. # Type of cap Color Qty/PkT320-2N Flat Natural 125T320-2B Flat Blue 125T320-2G Flat Green 125T320-2R Flat Red 125T320-2Y Flat Yellow 125


Cat. # Type of cap Color Qty/PkT320-3N Domed Natural 125T320-3B Domed Blue 125T320-3G Domed Green 125T320-3R Domed Red 125T320-3Y Domed Yellow 125

The cap has an integral seal preventing contaminationwith surface of lid.

This PCR reactionstrip is availablewith either flat(needle pierceable) or dome-toppedindividuallyattached hingedcaps.

PCR® FAMILY

T320-2 T320-3

T320-2 & -3 AMPLITUBE™

PCR REACTION STRIPSMade of polypropylene

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1 Alphanumeric grid for better identification2 Flexible plate for better fitting of tubes in thermal block3 Can be cut to desired format4 Corner is cut away to facilitate orientation of plate5 Inside of tube is smooth and has an inert surface

Amplate™ 96 Amplate™ 48 Amplate™ 24Cat. # Color Qty/Bag Qty/Cs Cat. # Cat. # Color Qty/Bag Qty/CsT323-96N Natural 10 100 T323-48N T323-24N Natural 10 50T323-96B Blue 10 100 T323-48B T323-24B Blue 10 50T323-96G Green 10 100 T323-48G T323-24G Green 10 50T323-96R Pink 10 100 T323-48R T323-24R Pink 10 50T323-96Y Yellow 10 100 T323-48Y T323-24Y Yellow 10 50

T323AMPLATE™ THIN WALL PCR PLATESMade of polypropylene

These 96-well PCR plates are thin-walled and designed for rapid thermal transfer. Each well has a capacity of 0.2 ml. They are precision-molded to ensure well-to-well and plat e - t o - p l ate uniformity. The insides of the tubes are smooth and have an inert surface on which enzymes and nucleic acids do not bind.

All sealing methods can be used for oil-free operat i o n : SecureSeal™ Thermal sealing film and foil (T329 Series), and A m p l ate™ Mat (T329-10). Suitable to beused with all 96-well shaped cy clers such as Ericomp Tw i n b l o c k , Perkin-Elmer 9600, Mj Research 100/200 96V etc... Their flexible design allows them to beeasily cut into sections of 24, 32 or 48 tubes.

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The AmplateTM 96 can be cutto any desired format.

The plates will accommodate differences in expansion coefficients between the metal thermal cycler block and polypropylene tubes. For moreconvenience, pre-cut plates are also available in the following formats: 48 tubes (6 x 8) and 24 tubes (3 x 8).

On the 96-well plate, an alphanumeric grid helps sample identification. To facilitate orientation, the bottom right corner of the plate is cut away. TheAMPLATE™ is easy to seal since no cylindrical walls extend above the plate. More economical than using single tubes, it is available colorless and in fourdifferent colors. Autoclavable. Packed in tamperproof resealable bags of 10 plates.

Amplate™ 24Amplate™ 48

T323-96

T323-48

T323-24

Amplate™ 96

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T323-96SKSKIRTED AMPLATE™ 96THIN WALL PCR PLATESMade of polypropylene

Similar to the T323 Series above, these skirted 96-well PCR plates arethin-walled and designed for rapid thermal transfer. The skirt around thep l ate provides a bar coding and label ing area, u n available in othertypes of plat e s . They are precision-molded to ensure well-to-well andp l at e - t o - p l ate uniformity. Quite superior to polycarbonate plat e s , t h e yare impermeable to water vap o r.

All sealing methods can be used for oil-free operat i o n : domed and flatc ap strips (T321 Series), SecureSeal™ Thermal sealing film and foil( T 3 2 9 ) , and A m p l ate™ Mat (T329-10 Series). Suitable to be used withall 96-well shaped cy clers such as Ericomp Tw i n b l o c k , Pe r k i n - E l m e r9 6 0 0 , Mj Research 100/200 96V etc...

An alphanumer i c gr id helps samp le ident i f ica t i o n . To faci l i ta t eo r i e n t a t i o n , t he top l e f t co rn e r o f t h e p l a te i s c u t a w a y. T h eA M P L ATE™ is easy to seal since no cylindrical walls extend above thep l a t e . Mo re economical than us ing s ing le tub es , i t i s av a i l a b l ecolorless an in four different colors.

Fi n a l l y, the S i m p o r t A M P L ATE™ can be handled by robotic handlingeq u ip men t an d i s i d ea l wi t h au tom a te d p ip e t t i n g s y st ems .A u t o cl av a b l e .

Packed in tamperproof resealable bags of 10 plat e s .Cat. # Color Qty/Bag Qty/CsT323-96SKN Natural 10 100T323-96SKB Blue 10 100T323-96SKG Green 10 100T323-96SKR Pink 10 100T323-96SKY Yellow 10 100

1 Alphanumeric grid for better identification2 Flat surface for better sealing3 Can be handled by robotic handling equipment4 Area for bar coding, labeling or writing on each side and top5 Each well has a volume of 100 μl

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This plate is a goodalternative to polycar-bonate plates made byGreiner and Costar

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T323-96LPLOW PROFILE AMPLATE™ 96THIN WALL PCR PLATESMade of polypropylene

These low prof i le 96-wel l PCR plates are simi lar to the regularS i m p o r t A M P L ATE™ Series detailed on the previous pag e . H ow e v e r,each of the 96 tubes has a smaller volume (only 100 μl) and therebyreduce the dead space between sample and cover.

Th ey ar e th in -wa l led an d d es ig ned fo r rap id the rma l trans fe r.precision-molded to ensure well-to-well and plat e - t o - p l ate uniformity.All sealing methods can be used for oil-free operat i o n : domed and flatc ap strips (T321 Series), SecureSeal™ Thermal sealing film and foil(T329 Series), and A m p l ate™ Mat (T329-10). Suitable to be used withall 96-well shaped cy clers such as Ericomp Tw i n b l o c k , Pe r k i n - E l m e r9 6 0 0 , Mj Research 100/200 96V etc...

T h e f l e x i b l e d es ig n ac co mm od a te s d i f f e r en ce s in ex p an s i oncoefficients between the metal thermal cy cler block and polypropylenet u b e s .

An alphan umer ic g ri d he lps sample i dent i f i ca t i o n . To fac i l i ta t eo r i e n t at i o n , two left corners of the plate are cut aw a y. The A M P L AT E ™is easy to seal since no cylindrical walls extend above the plat e . M o r eeconomical than using single tubes, it is available colorless and infour different colors. A u t o cl av a b l e .

Packed in tamperproof resealable bags of 10 plat e s .

1 Alphanumeric grid for better identification

2 Flat surface for better sealing

3 Small volume reducing dead space between sample and cover

CAN BE USED WITH ALL LEADING THERMAL CYCLERS

The Amplate™ 96LP can becut to any desired format.

Cat. # Color Qty/Bag Qty/CsT323-96LPN Natural 10 100T323-96LPB Blue 10 100T323-96LPG Green 10 100T323-96LPR Pink 10 100T323-96LPY Yellow 10 100

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AMPLATE™

RAISED RIM THIN WALL PCR PLATESMade of polypropylene

A m p l ate™ Raised Rim thin wall PCR plates are the latest addition to the wide range of Simport PCR products. Offering just the right rigidity fora u t o m at i o n , these three 96-well plat e s , made in a standard 8 x 12 configurat i o n , are perfectly suited for high performance thermal cy cl i n g . E a c hwell makes intimate contact with the heating block while quick and consistent heat transfer is ensured by a uniform wall thickness. Using aspecial polypropylene, samples are easily recovered thanks to a non-wettable surface. P l ates on all models can be cut in strips of 16 wells(2 x 8).

Well cap a c i t y : T323-100 and -101 Series: 250 μl, T323-103 Series: 200 μL

These plates offer the right alternative to existing Robbins, Corning and Perkin Elmer models. T323-100 and –101 Series have a 3 mm raised rimaround each tube well.

The latter is supplied with a wide skirt extending over and under the plate on which a bar code can be affixed to facilitate identificat i o n . S e r i e sT323-103 will also offer the same skirt but the rim above each tube well is only 1 mm high.

All sealing methods can be used for oil-free operat i o n : domed and flat cap strips (T321 Ser ies ) ; SecureSeal™ Thermal Sealing Film (T329-1);SecureSeal™ Aluminium Sealing Foil (T329-5) and A m p l ate™ Mat (T329-10). H owever Series T323-103 will accommodate micromats only. Tof a c i l i t ate orientat i o n , one corner of the plate is cut aw a y. An alphanumeric grid helps sample identificat i o n . Available colorless and in four pastelc o l o r s . A u t o cl av a b l e . Packed in temperproof resealable bags of ten bag s .

Cat. # Color Qty/Bag Qty/CsT323-100N Natural 10 100T323-100B Blue 10 100T323-100G Green 10 100T323-100R Pink 10 100T323-100Y Yellow 10 100



Our Latest Addition to the Wide Range of Simport PCR® Products

T323-101

T323-100

T323-103

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T323-384SKAMPLATE™ 384 THIN WALL PCR PLATESMade of polypropylene

This plate has been developed for high volume laborat o ry work. It isprecision-molded to ensure well-to-well and plat e - t o - p l ate uniformity.

The design of the A M P L ATE™ 384 is such that each well having a4 0 μl capacity can be used with reaction volumes from 2 to 30 μl. A l lwells on the plate are thin-walled to make sure that an efficient andfast heat transfer is occurring.

Al though i t has 384 we l ls , i t can be f i l led us ing automated f lu idhandling systems or standard multichannel pipettors.

I n o rd e r t o o f f e r mo re s u r f a ce c on tac tbetween the plate and the sealing medium,such as thermal foil and adhesive sealingf i l m s , t h e re a re n o cy l i nd r i ca l wa l l sextending above the plat e .

The A M P L ATE™ 384 is skirted to allowbar cod ing on s ides for identi f icat i o nand also to make i t compat ib le witha u t o m a ted f l u i d handl i ng systems.Holes on sides allow for preciseanda c c u r a t e p l a t e po s i t i on i n g an d

r e m o v a l .

An alphanumeric grid helps in locating the sample. Fi n a l l y,one corner of the plate is cut away to facilitate its orientat i o n .

The A M P L ATE™ 384 is defini tely more economical than usings i ng le tub es , s t r i p s , an d even 96-wel l p l at e s . I t is av a i l a b l e

colorless and in four popular colors. A u t o cl av a b l e .


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3 1 Can be filled using automated fluid handling systems2 Each well has only 40 μl capacity3 Can be handled by robotic handling equipment4 One corner is cut away to facilitate orientation5 Alphanumeric grid for better identification6 Area for bar coding, labeling or writing

Cat. # Color Qty/Bag Qty/CsT323-384SKN Natural 10 100T323-384SKB Blue 10 100T323-384SKG Green 10 100T323-384SKR Pink 10 100T323-384SKY Yellow 10 100

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T324-96SKOPAQUE SKIRTED AMPLATE™ 96THIN WALL PCR PLATESMade of polypropylene

T h e s e o p a q u e 9 6 - w e l l P C R p l a t e s a r e f o r c h e m i l u m i n e s c e n tand fluorescent procedures. Each well has a capacity of 100 μl. T h i n -walled and designed for rapid thermal transfer. They are precision-molded to ensure well-to-well and plat e - t o - p l ate uniformity.

All sealing methods can be used for oil-free operat i o n : domed and flatc ap strips (T321 Series), S e c u r e S e a lTM Thermal and foil (T329 Series),and A m p l ate™ Mat (T329-10). Suitable to be used with all 96-wells h aped cy clers such as Ericomp Tw i n b l o c k , Perkin-Elmer 9600, M jResearch 100/200 96V etc...

An alphanumeric grid helps in sample ident i ficat i o n . To faci li tat eo r i e n t a t i o n , t h e to p l e f t co rn e r o f t h e p l a te i s c u t a w a y. T h eA M P L ATE™ is easy to seal since no cylindrical walls extend above thep l at e . Fi n a l l y, the S i m p o r t A M P L ATE™ can be handled by robotichandling equipment and is ideal with automated pipetting systems.A u t o cl av a b l e .


T324-384SKOPAQUE SKIRTED AMPLATE™ 384THIN WALL PCR PLATESMade of polypropylene

For chemiluminescent and f luorescent procedures, the A M P L ATE™-384 is avai lab le in opaque white or b lack. The white p late wil lincrease signal output in both types of assays.

It has been developed for high volume laborat o ry work. It is precision-molded to ensure well-to-well and plat e - t o - p l ate uniformity.

The design of the A M P L ATE™ -384 is such that each well having a40 μl capacity can be used with react ion volumes from 2 to 30 μ lc ap a c i t y. On ly v i rg in po l ypropy lene i s us ed to manufacture thisp l at e . Although it has 384 wells, it can be filled using automated fluidhandling systems or standard multichannel pipettors.

All wells on the plate are thin-walled to make sure that an efficientand fast heat transfer is occurring.

In order to offer more surface contact between the plate and thesealing medium, such as thermal foil and adhesive sealing films, t h e r eare no cylindrical walls extending above the plat e .

The A M P L ATE™ -384 is skirted to allow bar coding on sides fori d e n t i f i c ation and also to make it compatible with automated fluidhandling systems. Holes on sides allow for precise and accurate plat epositioning and removal.

An alphanumeric grid helps in locating the sample. Fi n a l l y, one cornerof the plate is cut away to facilitate its orientat i o n . A u t o cl av a b l e .


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Cat. # Color Qty/Bag Qty/CsT324-384SKK Black 10 100T324-384SKW White 10 100

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2 Each well has only 40 μl capacity

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6 Can be handled by robotic handling equipment

7 Opaque to ensure low level of background fluorescence

8 Area for bar coding, labeling or writing

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Cat. # Color Qty/Bag Qty/CsT324-96SKK Black 10 100T324-96SKW White 10 100


2 Area for bar coding, labeling or writing

3 Can be handled by robotic handling equipment

4 One corner is cut away to facilitate orientation

5 Flat surface for better sealing

6 Opaque to ensure low level of background fluorescence


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T327COMBI-BOX™

The S i m p o r t Combi-Box™ can be used not only as as t o r age rack but also as a workstat i o n . The white basewill accept all 96- and 384-well plates and an easy toremove transparent cover al lows easy viewing of thec o n t e n t . Being only 40mm high, the stackable Combi-Box™ saves space on the lab bench and on refrigerat o ror freezer shelves.

For single tubes as well as strips, use the Combi-RackT M

(T327-1) which can hold up to 96 tubes or 12 strips of 8.

T327-1COMBI-RACK™


The S i m p o r t Combi-Rack™ is an innovative support that can hold upto 96 PCR tubes or 12 strips of 8 tubes with cap s . Each hole isident ified with an alphanumeric numbering system for identi fyingt u b e s . The grid stands on 4 legs and can be placed on a lab counter orin a refrigerator or freezer shelf. Made of polypropylene, it can easilywithstand temperatures from -80 °C to +121 ° C . It is also ideal forc a r r y i n g an d sto r i n g, f reez i n g and t r an sp o r t i n g r eag ent s an ds p e c i m e n s .

For storag e , simply place the Combi-Rack™ in the T327 Combi-Box™and place cover.

Cat. # Color Qty/CsT327-1 Blue 5

Cat. # Color Qty/CsT327 White base 5

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T328-96PCRack™


This convenient space saving rack was designed especially for storingand working with PCR samples. The PCRackT M will accept all models of0.2 ml tubes, along with strips of 8 or 12 tubes. 96-well PCR plat e scan also be accommodat e d .

The PCRackT M consists of a base and a cover. Both are made of extras t ro ng p o l yp r op y l en e to r es i s t a wi de ran g e o f c he mic a l s a tt e m p e r atures from -90 °C to 121 ° C . Samples are easily identified withth e PC RackT M a lph an umer i c con f i gura t i o n , in dex ed from A to Hvertically and 1 to 12 horizontally. The transparent cover also has easyto read numbers and letters for secure identification of contents.

The PCRackT M can be horizontally attached to each other in order tobuild-up any conf iguration you desire. With the cover on, they areeas i ly s tackable one on top of another. Thanks to these specia lf e a t u r e s , e f f i c i e n cy is h igh l y improved al l owing you to ca r ry amultitude of tubes and/or strips at the same time. A u t o cl av a b l e .

Available in 6 very attractive colors.

4

5

Space-saverOnly 40 mm high

Cat. # Color Qty/Cs Cat. # Color Qty/Cs

T328-96B Blue 20 T328-96R Red 20T328-96G Green 20 T328-96Y Yellow 20T328-96O Orange 20 T328-96AS Assorted* 20T328-96P Pink 20 *Blue, green, orange, pink, yellow.

6

4

1 Transparent cover for easy viewing of contents2 Boxes are stackable for space-saving3 Plate can easily be inserted and removed4 Alphanumeric identification of each position5 Can accept single tubes, strips and plates up to 96 wells6 Stackable

Will accept any PCR plate

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PCR® FAMILY

Properties of Simport SecureSealTM

Sealing Films and Foil

•Solvent loss after typical cy cle < 5% with no dry wells.

- The following cy cle repeated 35 times: 94 °C for 1 min;

55 °C for 1 min; 72 °C for 45 sec.

• Ve ry low autofluorescence for T329-1 polyolefin tap e .

•Adhesives do not interfere with cy cle reactions.

Adhesive films and foils are now increasing in popularity for covering a wide range ofp l ates in bioanalytical, genomic and pharmaceutical research.

S i m p o r t, with almost 30 years of experience creating innovative laborat o ry plasticwareand accessories, is offering a carefully selected range of sealing films and foil with thef o l l owing feat u r e s :

• L ow contamination of well contents by the tape adhesive•Prevention of evap o r ation from the individual wells•Clean tape removal for access to the well contents•Good optical properties for monitoring well contents through the film• Te m p e r ature resistance over wide ranges to include compound storage and PCR

The improved S i m p o r t S e c u r e S e a l T M F i lms and Foi l are higher qual i ty productsmanufactured through unequalled expertise and quality control.

T329-1 Polyolefin / T329-3 Polyester / T329-5 Aluminum

One hour direct contact between adhesive and solvent follow e dby overnight incubation at room temperat u r e .

S o l v e n t s : DMSO and ethanol (80) / water (20).

B l a n k s , c o n t r o l s , and extracts were analyzed by GC/MS.

R e s u l t s :D M S O : None detected above background.Ethanol / wat e r : Hydrocarbon acry l ate esters 5 μg /mL.

Antioxidant 630 μg /mL.

1 0 0

7 5

5 0

2 5

0C l a r i t y Tr a n s m i t t a n c e H a z e

Optical Property

T 3 2 9 - 1T 3 2 9 - 3

2 0

1 0

0

Water Evaporation Data (7 days)

At 22 °C

Product IdentificationT 3 2 9 - 5 T 3 2 9 - 1 T 3 2 9 - 3 Al Tap e PET Tap e

Initial Adhesion


3 0

2 0

1 0

0

1 0 09 08 07 06 05 04 03 02 01 00

Water Evaporation Data (24 Hours)

At 65 °C


1

0

- 1

DMSO Evaporation Data (7days)

At -70 °C


Optical Properties

Acetonitrile (80) / Water (20)E vaporation Data (7 days)

At 22 °C


8 0

7 0

6 0

5 0

4 0

3 0

2 0

1 00

Prevention of Water Eva p o r a t i o n Analysis of DMSO Mass Change

Adhesion to Polypropylene Plates

At 22 °C4 0

3 0

2 0

1 0

0

Isopropanol (80) / Water (20)E vaporation Data (7days)

At 22 °C


Prevention of Aqueous / Organic Solvent Eva p o r a t i o n

T he S im po r t S e cu r e Se a l T M T h e r m a lAdhesive Films prevent vapor loss andare thermostable and functional from-70 °C to +95 °C.

Solvent Extractables

PCR Compatibility

Se c u r eSe a l T M THERMAL ADHESIVE FILMS AND FOIL

PCR® FAMILY

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PCR® FAMILY

T329-1 & -2SecureSeal™ THERMAL ADHESIVE SEALING FILM forPCR application

This transparent sealing tape consists of a 2.0 mil polyolef in fi lmc o ated on one side with a pressure sensitive acry l ate adhesive whichdoes not interfere with cy cle reactions. It is ideal for reducing well-to-well contaminat ion and/or spil l over in sensi tive PCR ap p l i c at i o n swhere the minimization of evap o r ation and vapor loss is critical.

S e c u r e S e a lT M Thermal Film was developed with the assistance of amajor cy cler manufacturer for PCR ap p l i c at i o n s . Not only does it offerl ow - au to f l u o res c en ce b u t i t w i l l p r ev en t v a po r l o ss an d i sthermostable and funct ional from -70 °C to +95 °C. M a n u f a c t u r e dR N A s e , DNAse and DNA-free. DMSO resistant.

T329-10AMPLATE™ MAT (made of santoprene)

This flexible sealing cover is used on 96-well plates along with clip dow nand screw top thermal cy clers and has been proven to be a secure andeffective way of sealing. Since it is reusable, it is a nice way to make thisstep of the procedure cost effective. Dimples on one side of the matensure it is well placed over the tubes. A u t o cl av a b l e .

T329-9AMPLATE™ ROLLER

For ensuring a perfect seal when using either SecureSeal™ Thermal sealingfilm or foil on PCR plat e s . Roller made of medium hard rubber. H e av y - d u t yhandle with comfort grip reducing fatigue. Will last a long time.

T329-5SecureSeal™ ALUMINUM SEALING FOIL

This type of material is ideal for manual sealing during PCR work andalso for high throughput ap p l i c at i o n s . Adhesive backing makes it easyto ap p l y. Wi l l r es is t temp er a tu res f rom - 86 °C to + 95 ° C . I t i srecommended to use the A m p l at eT M Roller (T329-9) to ensure a perfectb o n d , e l i m i n ating the dangers of evap o r at i o n . Pierceable with a pipettip for easy access to sample. DMSO resistant.

Note: Performance may depend upon the specific collection/samplevessel used as well as the specific conditions subjected to.

Cat. # Color Sterile Qty/Pk Qty/CsT329-1 Transparent No 100 1000T329-2 Transparent Yes 100 1000

Cat. # Color Qty/PkT329-10 Blue 5

Cat. # Size Qty/PkT329-9 10.16 cm (4 in.) 1

Cat. # Description Qty/PkT329-5 Peeling foil 100 sheets

Prevents vapor loss

Transparent for easyviewing of contents

Functional from-70 ºC to +95 ºC

PCR® FAMILY

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PCR® FAMILY

PCR® FAMILY

THERMAL CYCLER GUIDET325 T320 T322 T323

Series Series Series SeriesManufacturer Thermal Cycler (Pages 68-69) (Pages 70-71) (Page 71) (Pages 72-76)

-1 -2 -3 -4 T320 T322 -24 -48 -96 -96LP -96SK -100 -101 -103 -384SK

Bio-Rad Gene Cycler • • •iCycler • • • • • • • • • • • • •

Biometra T Personnal • • • • •T1 Thermocycler • • • • • • • • • • • •T3 Thermocycler • • • • • • • •Uno • • • • • • • • •Uno II • • • • • • • • • • •Gradiant • • • • • • • • • • •

Corbett PC•960 • • • • • • • • • • • • •

Eppendorf Mastercycler • • • •Mastercycler Personnal • • • • • • • • • • •Mastercycler Gradient • • • • • • • • • • •Mastercycler 384 •

Ericom DeltaCycler I • • • • • • • • • • • •DeltaCycler II • • • • • • • • • • • •PowerBlock I • • • • • •Single Block • • • • • • • • • • • •Twin Block • • • • • • • • • • • •

Hybaid Omnigene • • • • • • • • • • •OMN•E • • • • • • • • • • • • •PCR Express • • • • • • • • • • • • • •Touchdown • • • • • • • • • • • • •

Idaho Tech. Indy • • • •

Lab-Line 210 • •212 • • • • • • • •214 • • • • • • • • • • •225 • •226 • • • • • • • • •227 • • • • • • • •

MJ Research PTC•100 • • • • • • • • • • • • •PTC•150 • • • •PTC•200 • • • • • • • • • • • • • •PTC•225 • • • • • • • • • • • • • •

MWG Primus 25 • • • •Primus 96 • • • • • • • • • • • •Primus 384 •

Oncor Crocodile III • • • • • • • • • • •

Operon OPR•PCR96 • • • • • • • • • • •

Perkin Elmer 480 • •2400 • • • • •2700 • • • • • • • • • • • •9600 • • • • • • • • • • •9700 • • • • • • • • • • • • • •Dual 384 •

Stratagene RoboCycler 40 • •RoboCycler 96 • • • • • • • • • •

Takara TP240 • • •TP3000 • • • • • • • • • • •

Techne Techgene • • • •Touchgene • • • • • • • • • • • • •Cyclogene • • • • • • • • • • • • •Genius • • • • • • • • • • • • • •Genius Quad • • • • • • • • • • • • • •

Thermolyne Amplitron II • • • • • • • • • • •

Documents

PCR F P C RFA M I LY & -4 AMPLITUBE™ PCR REACTION TUBES, 0.5 ml Made of polypropylene Also designed for oil-free opera it o n , the inside of these tubes has a polished s u r f a