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8/12/2019 Plant TC Media
1/18
Plant Tissue Culture Basal Salts and Media
Product
NumberProduct Description Product Notes
Package
Size
ANDERSON BASAL SALT MIXTUREon a ns e macro- an m cronu r en s as escr e y n erson , .
an ssue u ure es e
Storage Tempo u e n
- Ca er
B144 BANANA AGS BASAL MEDIUM
Contains the macro- and micronutrients, vitamins, and plant growth regulators required
to culture bananas.
Plant Tissue Culture Tested
Storage Temp
Soluble In
2-6C
ater
1 L
0 L
50 L
usa anana e um: ee - ase an pec c e um ec on
C206 CAPE SUNDEW/ VENUS FLY TRAP
MULTIPLICATION BASAL MEDIUM
Contains the macro- and micronutrients, vitamins, and plant growth regulators required
or e mu p ca on o carn vorous p an s.
an ssue u ure es e
Storage Temp
Soluble In
2-6C
ater
1 L
0 L
C216 CAPE SUNDEW/ VENUS FLY TRAP
PRETRANSPLANT BASAL MEDIUM
Contains the macro- and micronutrients, vitamins, and plant growth regulators required
for the growth of carnivorous plants.
an ssue u ure es e
Storage Temp
Soluble In
2-6C
ater
1 L
0 L
50 L
C212 CARROT CALLUS INITIATION BASAL MEDIUM
Contains the macro- and micronutrients, vitamins, and plant growth regulators required
to initiate carrot callus from pith tissue.
Plant Tissue Culture Tested
Storage Temp
Soluble In
2-6C
ater
1 L
0 L
50 L
on a ns e macro- an m cronu r en s, v am ns, an p an grow regu a ors requ re
to initiate shoots from carrot callus.
Plant Tissue Culture Tested
orage emp
o u e n
-
a er
50 L
C287 CHEE & POOL C2d VITIS BASAL MEDIUM
on a ns e macro- an m cronu r en s as escr e y ee oo .
an ssue u ure es e
Storage Temp
o u e n
- C
a er
C167 CHU N6 BASAL MEDIUM w/ VITAMINS
Contains the macro- and micronutrients, and vitamins as described by Chu (1975).
Plant Tissue Culture Tested
Storage Temp
Soluble In
2-6C
ater
1 L
0 L
on a ns e macro- an m cronu r en s as escr e y u .
Plant Tissue Culture Tested
orage emp
o u e n
-
a er
50 L
C149 CHU N6 VITAMIN SOLUTION (1000x)
See Vitamin Section (following the MS-based media section)
Contains macro- and micronutrients as described by Gupta & Durzan (1985).
Plant Tissue Culture Tested
orage emp
Soluble In
-
ater
0 L
50 L
Our Plant Tissue Culture Tested basal salts and mediahave been formulated according to the referencesc te n t e r respect ve pro uct st ngs. ese asa sa ts
and media are then manufactured and packaged in en-
vironmentally controlled facilities. Our biological testing
ensures t at eac ot pro uces cu ture growt cons stent
with prior lots.
On the following pages are many popular basal salt and
me a ormu as o owe y uras ge an oog- ase
formulations. Vitamin listings and formulations complete
this section. Please contact us with inquiries about larger
package sizes or custom formulations.
nstruct ons or asa sa t, me a, an v tam n preparat on
are provided in the Technical Information Section of this
catalog and on our web site: www.phytotechlab.com.
asal Salt vs Mediu ... our defnition
A Basal Salt Mixture contains macro- and micronutri-
ents, but no vitamins, carbohydrates, or growth regulators.
A Basal Medium contains the macro- and micronutrients,
ut s ncomp ete as t typ ca y ac s an organ c compo-
nent: vitamins, carbohydrates, or growth regulators. A
Medium typically is complete and requires no additional
components except ge ng agent, es re . o e
Basal Salt, Basal Medium, or Medium indicates that one
or more of the components varies in concentration or form
rom t at or g na y pu s e .
8/12/2019 Plant TC Media
2/18
All components expressed in
mg/L
An
dersonBasalSaltMixture
a
nana
asa
e
u
ape
unew
enus
y
rap
Multip.BasalMediu
Cap
eSundew/VenusFlyTrap
reranspan
asa
e
u
a
rro
ausn
aon
asa
e
u
CarrotShootDevelopment
BasalMediu
Chee&PoolC2dVitis
asa
e
u
u
asa
e
u
w/Vitamins
ChuN6BasalSaltMixture
asa
a
xure
Aluminum Chloride6H2O
Ammonium Nitrate
Ammonium Phosphate, Monobasic
mmon um u a e
orc c . . . . . . . .
Calcium Chloride, Anhydrous . . . . . . . .
Calcium Nitrate . .
Cobalt Chloride6H O . . . . . . . .
upr c u a e2
. . . . . . . .
a 2
. . . . . . . .
Ferric Chloride
Ferric Sodium EDTA . .
errous u a e2
. . . . . . . .
agnesum u a e, n y rous . . . . . . . . .
Manganese Chloride2O
Manganese SulfateH2O . . . . . . . .
Molybdic Acid (Sodium Salt)2H2O . . . . . . . .
o y enum r ox e
c e or e2
.
Nickel Sulfate2O
Potassium Chloride
Potassium Iodide . . . . . . . .
o ass um ra e
o ass um osp a e, asc
o ass um osp a e, ono asc
o ass u m u a e
o um ra e
Sodium Phosphate Monobasic .
Sodium Sulfate
nc ra e2
nc u a e2
. . . . . . . .
Adenine Hemisulfate
Glycine (Free Base)
2,4-Dichlorophenoxyacetic Acid
- , me y a y am nopur ne
n o e- -ace c c
ne n .
L(-)Malic Acid
myo-Inositol
co n c c ree c .
yr ox ne .
amneHCI . . .
Grams of powder to prepare 1 liter . . . . . . . . . .
pH0.5 at RT . . . . . . . . . .
NS = No Specication Established rev 11/2005
8/12/2019 Plant TC Media
3/18
3
Plant Tissue Culture Basal Salts and Media (continued)
Product
NumberProduct Description Product Notes
Package
Size
Contains the macro- and micronutrients as described by Driver & Kuniyuki (1984) and
McGranahan, et al (1987).
orage emp
Soluble In
-
Water
10 L
50 L
DKW BASAL MEDIUMon a ns g ucrose; w ou am ns
on a ns macro- an mcronu r en s as escr e y r ver un yu an
c rana an, e a . .
Plant Tissue Culture Tested
Storage Tempo u e n - Ca er
DKW BASAL MEDIUM
on a ns g ucrose; w ou v am ns
on a ns macro- an mcronu r en s as escr e y r ver un yu an
McGranahan, et al. (1987).
Plant Tissue Culture Tested
Storage Temp
o u e n
- C
a er
ERIKSSON VITAMIN SOLUTION (1000x)
ee tamn ecton o ow ng t e - ase me a secton
Contains the macro- and micronutrients as described by Gamborg, et al. (1968).
Plant Tissue Culture Tested
orage emp
Soluble In
-
Water
10 L
50 LG398 GAMBORG B-5 BASAL MEDIUM
on a ns e macro- an mcronu r en s, an v amns as escr e y am org, e a .
.
Plant Tissue Culture Tested
Storage Temp
o u e n
- C
a er
G359 GAMBORG (PRL-4-DM) LONG BASAL MEDIUM
Contains the macro- and micronutrients, and vitamins as described by Gamborg, et al.
.
an ssue u ure es e
Storage Temp
Soluble In
2-6C
a er
1 L
G249 GAMBORG VITAMIN POWDER (1000x)
See Vitamin Section (following the MS-based media section)
x
ee tamn ecton o ow ng t e - ase me a secton
G371 GRESSHOFF & DOY BASAL MEDIUMContains the macro- and micronutrients as described by Gresshoff & Doy (1974).
Plant Tissue Culture Tested
Storage TempSoluble In
2-6CWater
1 L10 L
50 L
on a ns e macro- an m cronu r en s as escr e y e er .
an ssue u ure es e
orage emp
o u e n
-
a er
H396 HELLER/ WHITE MODIFIED BASAL SALT MIXTURE
Contains the macro- and micronutrients as described by Heller (1953) and White
. ou o y enum r ox e
an ssue u ure es e
Storage Temp
Soluble In
2-6C
a er
1 L
10 L
H353 HOAGLAND MODIFIED BASAL SALT MIXTURE
Contains Ferrous Sulfate
Contains the macro- and micronutrients as described by Hoagland and Arnon (1938).
Plant Tissue Culture Tested
Storage Temp
Soluble In
2-6C
Water
1 L
10 L
50 L
e a
See MS-Based Plant-Specic Media (following this section)
K413 KAO & MICHAYLUK BASAL SALT MIXTURE
Contains the macro- and micronutrients as described by Kao & Michayluk (1975).
an ssue u ure es e
Storage Temp
Soluble In
2-6C
a er
1 L
10 L
Contains the macro- and micronutrients, vitamins, and organic acids as described by
Kao & Michayluk (1975).
Plant Tissue Culture Tested
orage emp
Soluble In
-
Water
10 L
50 L
x
ee tamn ecton o ow ng t e - ase me a secton
8/12/2019 Plant TC Media
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4
components
expressed in mg/L
DKWBasalSal
xture
DKWBasal
Mediumw/10g/
Sucrose
DKWBasal
Medium
w/30g/
Sucrose
GamborgBasal
SaltMixture
GamborgB-5
BasalMediu
Gamborg
(PRL-4-DM)Long
BasalMediu
Gresshoff&Do
BasalMediu
HellerBasalSalt
Mixture
Heller/White
ModiedBasal
SaltMixture
HoaglandModied
BasalSaltMixture
Kao&Michayluk
BasalSaltMixture
Kao&Michayluk
ModiedBasal
Medium
COMPONENT D190 D191 D189 G768 G398 G359 G371 H393 H396 H353 K413 K427
Aluminum Chloride6H O .0543 0.03
Ammonium Nitrate 1416 416 1416 1000 600 600
Ammonium Phosphate,Monobasic 115.03
Ammonium Sulfate 134 34 00
Boric Acid 4.8 4.8 .8 3 3 0.3 1 1.24 2.86 3
Calcium Chloride, Anhydrous 112.5 12.5 112.5 113.24 113.24 113.24 6.7 453 453
Calcium Nitrate 1367 367 1367 241.2 300 56.4
Cobalt Chloride6H O .025 .025 .25 0.025 0.025 .025
Cupric Sulfate5H2O .25 0.25 0.25 .025 .025 .25 0.025 .03 0.03 0.08 0.025 .025
Na2EDTA2H
2O 45.4 45.4 45.4 7.26 7.26 186.0 37.25 3.35 37.26 7.26
Ferric Chloride 1
Ferrous Sulfate7H2O 3.8 33.8 33.8 27.8 27.8 139.0 27.85 25 .5 27.85 27.85
Magnesium Sulfate, Anhydrous 361.49 361.49 361.49 122.09 122.09 122.09 17.099 122.1 351.63 240.76 146.55 146.55
Manganese Chloride4H O 1.81
Manganese SulfateH O 3.5 33.5 33.5 10 10 132.0 1 .0758 0.01 10 10
Molybdic Acid (Sodium Salt)2H2O .39 0.39 0.39 0.25 0.25 .25 0.025 .25 0.25
Molybdenum Trioxide .016
Nickel Chloride .03 0.03
Nickel Sulfate6H O 0.005 .005 .005
Potassium Chloride 00 5 750 65 300 300
Potassium Iodide 0.75 0.75 .75 0.8 .01 0.01 .75 0.75
Potassium Nitrate 2500 2500 1000 1000 80 06.6 1900 900
Potassium Phosphate, Monobasic 65 265 265 300 170 170
Potassium Sulfate 1559 559 1559
Sodium Nitrate 600
Sodium Phosphate Monobasic 150 50 0.0 108.75 16.5
Sodium Sulfate 200
inc Nitrate6H2O 17 17 17
inc Sulfate7H2O 2 3 0.3 1 1 0.22 2 2
p-Aminobenzoic Acid 0.2 0.02
L-Arginine (Free Base) 40
L-Ascorbic Acid 0.4 2
Asparagine 40
D-Biotin .00025 0.2 0.01
D-Calcium Pantothenate 0.4 1
Choline Chloride 0.2 1
Citric Acid (Free Acid) Anhydrous 40
Cyancobalamin, Vit B12
0.02
Folic Acid 0.015 .4
Fumaric Acid 40
L-Glutamine 60
Glycine (Free Base) 20 4
L(-)-Maleic Acid
L-Methionine 0.0
myo-Inositol 00 00 10 100
Nicotinamide 1
Nicotinic Acid (Free Acid) 1 0.5 0.1
L-Phenylalanine 20
PyridoxineHCI 1 0.5 0.1 1
Pyruvic Acid 20
Riboavin 0.015 .2
Sucrose 10,000 30,000
ThiamineHCI 10 0.5 1 1
L-Tryptophan 40
itamin A 0.01
itamin D3
0.01
Grams of powder to prepare 1 liter .22 15.22 5.22 3.10 3.21 .64 2.71 .64 1.04 1.63 .65 .9
pH0.5 at RT 4.3 4.0 .1 4.0 4.0 NS 3.9 4.9 4.7 .5 4.0 .4NS = No Specication Established
rev
8/12/2019 Plant TC Media
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5
Product
NumberProduct Description Product Notes
Package
Size
s me um may e so as uras ge oog e um w nm a rgancs
(MSMO) by some media suppliers.
Contains the macro- and micronutrients, and vitamins as described by Linsmaier &
Skoog (1965).
Plant Tissue Culture Tested
orage emp
o u e n
-
a er
50 L
pH Adjusted and Buffered
This medium may be sold as Murashige & Skoog Medium with Minimal Organics
(MSMO) by some media suppliers.
Contains the macro- and micronutrients, and vitamins as described by Linsmaier &
oog .
an ssue u ure es e
orage emp
Soluble In
-
Water
10 L
50 L
L467 LINSMAIER & SKOOG BASAL MEDIUM
w/ 30 g/L SUCROSE & 7 g/L AGAR
Contains the macro- and micronutrients, and vitamins as described by Linsmaier &
Skoog (1965).
an ssue u ure es e
Storage Temp
Soluble In
2-6C
Water
1 L
10 L
50 L
L452 LINSMAIER & SKOOG MODIFIED BASAL MEDIUM
w/ 30 g/L SUCROSE & 7 g/L AGAR
pH Adjusted and BufferedContains the macro- and micronutrients, and vitamins as described by Linsmaier &
oog .
an ssue u ure es e
Storage Temp
Soluble In
2-6C
Water
1 L
10 L
50 L
L154 LLOYD & McCOWN WOODY PLANT BASAL SALT MIXTURE
Contains the WPM macro- and micronutrients as described by Lloyd & McCown
(1981).
Plant Tissue Culture Tested
Storage Temp
Soluble In
2-6C
Water
1 L
10 L
50 L
c w
on a ns e macro- an mcronu ren s, an v amns as escr e y oy
McCown (1981).
Plant Tissue Culture Tested
orage emp
o u e n
-
a er
50 L
c
on ans e m c ronu rens escr e y oy c own .
an ssue u ure es e
orage emp
o u e n
-
a er
M419 MG BASAL SALT MIXTURE
Modied Murashige & Skoog/ Gamborg Basal Salt Mixture
Plant Tissue Culture Tested
Storage Temp
Soluble In
2-6C
Water
1 L
10 L
See Murashige & Skoog-Based Basal Salts & Media Section (following this section)
N492 NB BASAL MEDIUM
Modied Chu/ Gamborg Basal Medium
on a ns e macronu r en s as escr e y u an e m cronu r en s as
escr e y am org, e a . .
an ssue u ure es e
Storage Temp
Soluble In
2-6C
a er
1 L
10 L
N613 NITSCH & NITSCH BASAL SALT MIXTURE
Contains the macro- and micronutrients as described by Nitsch & Nitsch (1969).
Plant Tissue Culture Tested
Storage Temp
Soluble In
2-6C
Water
1 L
10 L
w
Contains the macro- and micronutrients, and vitamins as described by Nitsch & Nitsch
(1969).
Plant Tissue Culture Tested
orage emp
Soluble In
-
Water
10 L
50 L
x
ee tamn ecton o ow ng t e - ase me a secton
N603 NITSCH & NITSCH VITAMIN SOLUTION (1000x)
See Vitamin Section (following the MS-based media section
8/12/2019 Plant TC Media
6/18
All components
expressed in mg/L
Linsmaier&Skoog
asa
e
u
nsmaer
oog
o
easa
e
um
(MSMO)
Linsmaier&SkoogBasal
Mediu
Linsmaier&Skoog
ModiedBasalMedium,
uerep
use
Lloyd&McCownWoody
PlantBasalSaltMixture
Lloyd&McCownWoody
PlantBasalMedium
oy
cown
ooy
PlantMicronutrientMixture
MGBasalSaltMixture
(ModiedMS/Gamborg
BasalSaltMixture)
asa
e
u
sc
scasa
a
Mixture
Nitsch&NitschBasal
e
u
mmon um ra e
Ammonium Sulfate .
Boric Acid . . . . . . . .
Calcium Chloride, Anhydrous . . . . . . . . .
a cum ra e
o a or e2
. . . . . .
Cupric Sulfate5H2O . . . . . . . . . . .
Na2EDTA2H
2O . . . . . . . . . . .
Ferrous Sulfate7H O . . . . . . . . . . .
agnesum u a e, n y rous . . . . . . . . . . .
anganese u a e2
. . . . . . . . . .
Molybdic Acid (Sodium Salt)2H2O . . . . . . . . . . .
Potassium Hydroxide
o ass um o e . . . . . .
o ass um ra e
o ass um osp a e, ono asc .
Potassium Sulfate
Sodium Nitrate .
o um osp a e ono as c .
nc u a e2
. . . . . . . .
gar
D-Biotin .
Folic Acid .
yc ne ree ase
ree c
myo- nos o
Nicotinic Acid (Free Acid) .
PyridoxineHCI . .
ucrose , ,
amne . . . . .
Grams of powder to prepare 1 liter . . . . . . . . . . .
pH0.5 at RT NS . NS NS NS NS . . . NS
NS = No Specication Established rev 11/2005
8/12/2019 Plant TC Media
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7
Plant Tissue Culture Basal Salts and Media (continued)
Product
NumberProduct Description Product Notes
Package
Size
Contains the macro- and micronutrients, and organic constituents as described by
Lichter (1982); without sucrose.
Plant Tissue Culture Tested
orage emp
Soluble In
-
Water
10 L
50 L
on a ns e macro- an mcronu r en s as escr e y c o , e a . .
ormu a e or e grow o eratopter s r c ar .
Plant Tissue Culture Tested
orage emp
o u e n
-
a er
Q673 QUOIRIN & LEPOIVRE BASAL SALT MIXTURE
on a ns e macro- an m cronu r en s as escr e y uo r n epo vre an
uo rn, e a . .
an ssue u ure es e
Storage Temp
o u e n
2-6C
a er
1 L
R756 ROSE MODIFIED INITIATION BASAL MEDIUM
Stage I
Contains FeNaEDTA in place of Ferrous Sulfate and Disodium EDTA
an ssue u ure es e
Storage Temp
Soluble In
2-6C
Water
1 L
10 L
R757 ROSE MODIFIED MULTIPLICATION BASAL MEDIUM
Stage IIContains FeNaEDTA in place of Ferrous Sulfate and Disodium EDTA
Plant Tissue Culture Tested
Storage Temp
Soluble In
2-6C
Water
1 L
10 L50 L
age
Contains FeNaEDTA in place of Ferrous Sulfate and Disodium EDTA
Plant Tissue Culture Tested
orage emp
o u e n
-
a er
50 L
S816 SCHENK & HILDEBRANDT BASAL SALT MIXTURE
on a ns e macro- an m cronu r e n s as escr e y c en an e ran
.
an ssue u ure es e
Storage Temp
o u e n
- C
a er
S811 SCHENK & HILDEBRANDT MODIFIED BASAL MEDIUM
Contains 10 g/L Sucrose; Without Vitamins
an ssue u ure es e
Storage Temp
Soluble In
2-6C
a er
1 L
10 L
Contains 10 g/L sucrose, x vitamins, x micro- and x macronutrients.
Plant Tissue Culture Tested
orage empSoluble In
- Water
10 L
50 L
S806 SCHENK & HILDEBRANDT MODIFIED BASAL SALT MIXTURE
ou ac um
an ssue u ure es e
Storage Temp
o u e n
- C
a er
S813 SCHENK & HILDEBRANDT MODIFIED BASAL MEDIUM
Contains vitamins and 10g/L sucrose.
Plant Tissue Culture Tested
Storage Temp
Soluble In
2-6C
Water
1 L
10 L
50 L
x
ee tamn ecton o ow ng t e - ase me a secton
8/12/2019 Plant TC Media
8/18
componen s
expressed in mg/Lasa
e
um
arer-
ompson
asc
ern
asa
a
xure
Quoirin&LepoivreBasal
SaltMixture
RoseModiedInitiation
asa
e
u
ose
o
e
u
pcaon
asa
Mediu
RoseModiedRooting
BasalMediu
Schenk&Hildebrandt
asa
a
xure
cen
eran
o
easa
e
u
Schenk&Hildebrandt
ModiedBasalMediu
Schenk&Hildebrandt
ModiedBasalSalt
xure
cen
eran
o
easa
e
u
COMPONENT 479 713 673 756 757 758 S816 S811 S808 S806 813
Ammonium Molybdate .
Ammonium Nitrate .
Ammonium Phosphate,Monobasic
orc c . . . . . . . . . .
Calcium Chloride, Anhydrous . . .
Calcium Nitrate .
o a or e2
. . . . . . . . . .
upr c u a e2
. . . . . . . . . . .
Na EDTA2H O . .
Ferric Sodium EDTA . . . .
Ferrous Sulfate7H O . . .Magnesium Sulfate, Anhydrous . . . . . . . .
anganese u a e2
. . . . . .
o y c c o um a 2
. . . . . . . . . .
o ass um o e . . . . . . . . .
Potassium Nitrate
Potassium Phosphate, Monobasic .
nc u a e2
. . . . . . . . . .
- scor c c
6-Benzylaminopurine (BA) . .
D-Biotin .
r c c ree c n y rous
o c c .L-Glutamine
Glutathion
Glycine (Free Base) . . . .
n o e- -ace c c . .
myo- nos o
- ap a eneace c c .
Nicotinic Acid (Free Acid) . . . . . .
PyridoxineHCI . . . . . .
- er ne
ucrose
amneHCI . . . . . .
Grams of powder to prepare 1 liter . . . . . . . . . . .pH0.5 at RT . . . . . . . . . . .
= o pec ca on s a s e
rev 11/2005
8/12/2019 Plant TC Media
9/18
9
Plant Tissue Culture Basal Salts and Media (continued)
Product
NumberProduct Description Product Notes
Package
Size
Plant Tissue Culture Tested
orage emp
Soluble In
-
Water
10 L
50 L
TM4G BASAL MEDIUM
an ssue u ure es e
Storage Tempo u e n - Ca er
T856 TOBACCO MODIFIED CALLUS INITIATION BASAL MEDIUM
Contains FeNaEDTA in place of Ferrous Sulfate and Disodium EDTA
Plant Tissue Culture Tested
Storage Temp
Soluble In
2-6C
Water
1 L
10 L
50 L
on a ns e a n p ace o errous u a e an so um
Plant Tissue Culture Tested
orage emp
o u e n
-
a er
50 L
T867 TOBACCO MODIFIED SHOOT & ROOT BASAL MEDIUM
Contains FeNaEDTA in place of Ferrous Sulfate and Disodium EDTA
an ssue u ure es e
Storage Temp
Soluble In
2-6C
a er
1 L
T861 TOBACCO MODIFIED ROOT INITIATION BASAL MEDIUM
Contains FeNaEDTA in place of Ferrous Sulfate and Disodium EDTA
Plant Tissue Culture Tested
Storage Temp
Soluble In
2-6C
Water
1 L
10 L
50 L
on a ns e macro- an mcronu r en s, an v amns as escr e y o e .
an ssue u ure es e
orage emp
o u e n
-
a er
W865 WESTVACO WV5 BASAL MEDIUM
on a ns e macro- an mcronu r en s, an v amns as escr e y o e a .
an ssue u ure es e
Storage Temp
o u e n
2-6C
a er
1 L
W898 WHITE BASAL SALT MIXTURE
Contains the macro- and micronutrients as described by White (1963).
Plant Tissue Culture Tested
Storage Temp
Soluble In
2-6C
Water
1 L
10 L
Murashige & Skoog Basal Salts and Media following this section.
8/12/2019 Plant TC Media
10/18
0
All components expressed
in mg/Lasa
a
xure
TM4GBasalMediu
oacco
o
eaus
n
aon
asa
e
u
TobaccoModiedShoot
MultiplicationBasal
e
u
oacco
o
e
oo
oo
asa
e
u
TobaccoModiedRoot
InitiationBasalMediu
esvaco
asa
e
u
WestvacoWV5Basal
e
u
e
asa
a
xure
OMPONENT T853 T868 T856 T864 T867 T861 863 865 898
Ammonium Nitrate
mmon um osp a e, ono as c
mmon um u a e
Boric Acid . . . . . . . . .
Calcium Chloride, Anhydrous . . . .
a cum ra e .
o a or e2
. . . . . . . .
Cupric Sulfate2O . . . . . . . . .
Na2EDTA2H
2O . .
Ferric Sodium EDTA . . . . . .
err c u a e .errous u a e . .
Magnesium Sulfate, Anhydrous . . . . .
Manganese SulfateH2O . . . . . . . . .
Molybdenum Trioxide .
o y c c o um a 2
. . . . . . . .
o ass um or e . . .
o ass um o e . . . . . . . . .
Potassium Nitrate . . .
Potassium Phosphate, Monobasic
o um osp a e ono as c .
o um u a e
Zinc Sulfate7H2O . . . . . . . . .D-Biotin .
Casein, Enzymatic Hydrolysate
o c c .
yc ne ree ase . . . . .
Indole-3-acetic Acid . . .
Kinetin . . .
myo- nos o
co n c c ree c . . . . .
yr ox neHCI . . . . .
ThiamineHCI . . . . . . .
Grams of powder to prepare 1 liter . . . . . . . .
p . a . . . .= o pec ca on s a s e rev
8/12/2019 Plant TC Media
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1
Murashige & Skoog-Based Basal SaltsProduct
NumberProduct Description Product Notes
Package
Size
Contains the macro- and micronutrients as described by Murashige & Skoog (1962).
Plant Tissue Culture Tested
See Product No. M519 for Murashige & Skoog Basal Medium w/ Vitamins
orage emp
Soluble In
-
Water
0 L
50 L
on a ns e a
Plant Tissue Culture Tested
orage empo u e n
- a er
50 L
M502 MURASHIGE & SKOOG MACRONUTRIENT SALT BASE
Contains the macronutrients as described by Murashige & Skoog (1962).
an ssue u ure es e
Storage Temp
Soluble In
2-6C
a er
1 L
x
Contains the macronutrients as described by Murashige & Skoog (1962).
Plant Tissue Culture Tested
orage emp
Soluble In
-
Water
0 L
50 L
on a ns e m cronu r en s as escr e y uras ge oog .
an ssue u ure es e
orage emp
o u e n
-
a er
M529 MURASHIGE & SKOOG MICRONUTRIENT STOCK SOLUTION (10x)
Contains the micronutrients as described by Murashige & Skoog (1962).Plant Tissue Culture Tested
Storage Temp
Soluble In
2-6C
Water
1 L
0 L
Contains x macro- and x micronutrients as described by Murashige & Skoog
(1962).
Plant Tissue Culture Tested
orage emp
Soluble In
-
Water
0 L
50 L
on a ns x mmon um ra e an x o ass um ra e, w e rema n ng macro-
an mcronu r en s as escr e y uras ge oog .
Plant Tissue Culture Tested
orage emp
o u e n
-
a er
M290 MURASHIGE & SKOOG MODIFIED BASAL SALT MIXTURE
Contains x Ammonium Nitrate, x Potassium Nitrate, and x Calcium Chloride, with
e rema n ng macro- an m cronu r en s as escr e y uras ge oog .
an ssue u ure es e
Storage Temp
Soluble In
2-6C
a er
1 L
M571 MURASHIGE & SKOOG MODIFIED BASAL SALT MIXTURE
Without Ammonium Nitrate. Contains the remaining macro- and micronutrients as
described by Murashige & Skoog (1962).
an ssue u ure es e
Storage Temp
Soluble In
2-6C
Water
1 L
0 L
50 L
Without Nitrogen. Contains the macro- and micronutrients as described by Murashige
& Skoog (1962), modied by eliminating NH4NO
3and KNO .
Plant Tissue Culture Tested
orage emp
Soluble In
-
Water
0 L
50 L
ou rogen, osp orous, an o ass um
Contains the remaining macro- and micronutrients as described by Murashige & Skoog
(1962).
Plant Tissue Culture Tested
orage emp
o u e n
-
a er
50 L
8/12/2019 Plant TC Media
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2
componen s
expresse n mg
uras
ge
oog
asa
a
xure
M
SModiedBasalSalt
Mixturew/FeNaEDTA
MS
MacronutrientSaltBase
acronuren
oc
ouon
x
cronuren
aase
M
SMicronutrientStock
Solution(10x)
o
easa
a
xure
(
1/2xMicros&Macros)
M
SModiedBasalSalt
Mixture(1/2xNitrates)
M
SModiedBasalSalt
Mixture
o
easa
a
Mixture(NoNHNO3)
o
easa
a
xure
o
rogen
M
SModiedBasalSalt
xure
o
,,or
COMPONENT M524 M499 M502 M654 M554 M529 M153 M561 M290 M571 M531 M407
mmon um ra e
orc c . . . . . . . . .
Calcium Chloride, Anhydrous . . . . . . . .
Cobalt Chloride6H O . . . . . . . . . .
upr c u a e2
. . . . . . . . . .
a2
2O . . . . . . . .
Ferric Sodium EDTA .
Ferrous Sulfate7H O . . . . . . . .
agnesum u a e, n y rous . . . . . . . .
Manganese SulfateH O . . . . . . . . .
o y c c o um
a 2
. . . . . . . . . .
Potassium Iodide . . . . . . . . . .
o ass um ra e
Potassium Phosphate,
Monobasic
Zinc Sulfate2O . . . . . . . . .
Grams of powder to prepare
er
. . . . . . . . . .
pH0.5 at RT . NS NS . . . NS . NS NS . .
NS = No Specication Established Rev. 11/2005
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3
Murashige & Skoog-Based MediaProduct
NumberProduct Description Product Notes
Package
Size
w
e macro- an m cronu r en s, an v am ns as escr e y uras ge oog
(1962).
Plant Tissue Culture Tested
Contains the basal salts of Product No. M524
orage emp
o u e n
-
a er
50 L
ou o ass um osp a e; con a ns e a n p ace o errous u a e an
Disodium EDTA. Contains the remaining macro- and micronutrients, and modied vitamins
as described by Murashige & Skoog (1962). Also contains (mg/L): 300 Sodium Phosphate
Monobasic, 150 Adenine Hemisulfate, and 1000 Casein Hydrolysate.
Plant Tissue Culture Tested
orage emp
o u e n
-
a er
50 L
w
e macro- an m cronu r en s as escr e y uras ge oog , an
vitamins as described by Gamborg, et al. (1966).
Plant Tissue Culture Tested
orage emp
o u e n
-
a er
50 L
on a ns e macro- an m cronu r en s as escr e y uras ge oog ;
mo e w e a on o mg : . an .
Plant Tissue Culture Tested
orage emp
o u e n
-
a er
M701 MURASHIGE & SKOOG MODIFIED BASAL MEDIUM
This medium was formerly listed as M506.
s me um may e so as uras ge egona u p ca on e um y some me a
supp ers. on a ns e macro- an m cronu r en s as escr e y uras ge oog
; mo e w e a on o mg : an en ne em su a e.
Plant Tissue Culture Tested
Storage Temp
Soluble In
2-6C
a er
1 L
M702 MURASHIGE & SKOOG MODIFIED MEDIUM
Contains the macro- and micronutrients as described by Murashige & Skoog (1962);
mo e w e a on o mg : an en ne em su a e, an .
an ssue u ure es e
Storage Temp
Soluble In
2-6C
a er
1 L
M535 MURASHIGE & SKOOG MODIFIED BASAL MEDIUM
Without Nicotinic Acid, PyridoxineHCl, and Glycine
Contains the macro- and micronutrients as described by Murashige & Skoog (1962);
mo e w e a on o mg . am ne , myo- nos o , an en ne
em su a e.an ssue u ure es e
Storage Temp
Soluble In
2-6C
Water
1 L
10 L
MURASHIGE MEDIUM WITH MINIMAL ORGANICS (MSMO)
See L689 & L477 Linsmaier & Skoog Basal Medium
on a ns e macro- an m cronu r en s, an v am ns as escr e y uras ge oog
(1962); modied with the addition of (mg/L): 2.0 2iP, 80 Adenine Hemisulfate, and 170
Sodium Phosphate.
Plant Tissue Culture Tested
orage emp
o u e n
-
a er
50 L
s me um may e so as uras ge oog oo u p ca on e um y some
me a supp ers. on a ns e macro- an m cronu r en s as escr e y uras ge
Skoog (1962); modied with the addition of (mg/L): 1.0 Kinetin, 80 Adenine Hemisulfate,
and 148 Sodium Phosphate Monobasic.
Plant Tissue Culture Tested
orage emp
o u e n
-
a er
s me um s so as uras ge oo p oo ng e um y some me a supp ers.
Contains the macro- and micronutrients as described by Murashige & Skoog (1962) and
vitamins as described by Linsmaier & Skoog (1965); modied with the addition of (mg/L):
0.3 IAA, 1.0 Kinetin, and FeNaEDTA.
Plant Tissue Culture Tested
orage emp
o u e n
-
a er
50 L
s me um s so as oo u p ca on e um y some me a supp ers.
Contains the macro- and micronutrients as described by Murashige & Skoog (1962) and
vitamins as described by Linsmaier & Skoog (1965); modied with the addition of (mg/L):
170 Sodium Phosphate Monobasic, 80 Adenine Hemisulfate, 30 2iP, and 0.3 IAA.
Plant Tissue Culture Tested
orage emp
o u e n
-
a er
50 L
ee tamn ect on o owng t s sect on
8/12/2019 Plant TC Media
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4
All components
expressed in mg/L
uras
ge
oog
asa
e
u
o
easa
e
um
(w/outKH2PO4)
MSModiedBasalMediu
w/GamborgVitamins
o
ee
umw
o
easa
e
um
w/2iP
MS
ModiedMediumw/2iP
&IAA
o
easa
e
u
uras
ge
o
e
Mu
ltiplicationBasalMediu
w/2iP
M
SModiedMultiplication
Mediumw/Kinetin
MurashigeModied
u
pcaon
asa
e
um
wnen
M
urashigeModiedShoot
Mu
ltiplicationBasalMediu
OMPONENT 519 M541 404 401 M701 702 535 M536 555 527 491
mmon um ra e
orc c . . . . . . . . . . .
Calcium Chloride, Anhydrous . . . . . . . .
Cobalt Chloride6H O . . . . . . . . . . .
Cupric Sulfate5H O . . . . . . . . . . .
a2
2
. . . . . . .
err c o um . . . .
Ferrous Sulfate7H2O . . . . . . .
Magnesium Sulfate, Anhydrous . . . . . . . .
Manganese SulfateH O . . . . . . . . . . .
o y c c o um a 2
. . . . . . . . . . .
o ass um o e . . . . . . . . . . .
Potassium Nitrate
Potassium Phosphate, Monobasic
o um osp a e ono as c
nc u a e . . . . . . . . . . .
Adenine Hemisulfate
6-Benzylaminopurine (BA)
Casein, Enzymatic Hydrolysate
yc ne ree ase .
6-, Dimethylallylaminopurine (2iP)
n o e- -ace c c . . .Indole-3-butyric Acid
Kinetin
myo-Inositol
- ap a eneace c c . .
co n c c ree c . . .
PyridoxineHCI . . .
Sucrose
am ne . . . . . . . . . .
rams o pow er o prepare er . . . . . . . . . . .
p . a . . . . . . . . .
NS = No Specication Established Rev 11/2005
8/12/2019 Plant TC Media
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5
MS-Based Plant-Specifc Media
Product
NumberProduct Description Product Notes
Package
Size
Contains FeNaEDTA in place of Ferrous Sulfate and Disodium EDTA. Contains the
remaining macro- and micronutrients as described by Murashige & Skoog (1962).
Plant Tissue Culture Tested
orage emp
o u e n
-
a er
50 L
Contains FeNaEDTA in place of Ferrous Sulfate and Disodium EDTA. Contains the
remaining macro- and micronutrients as described by Murashige & Skoog (1962).
Plant Tissue Culture Tested
orage emp
o u e n
-
a er
50 L
ra ops s u ure e um
o e w e a on o mg : . , - , an . ne n
Plant Tissue Culture Tested
orage emp
o u e n
-
a er
M506 MURASHIGE BEGONIA MULTIPLICATION MEDIUM
ee ro uc o. 1 uras ge oog o e e um same ormua on
Contains FeNaEDTA in place of Ferrous Sulfate and Disodium EDTA. Contains the
remaining macro- and micronutrients as described by Murashige & Skoog (1962).
Plant Tissue Culture Tested
orage emp
Soluble In
-
Water
10 L
50 L
on a ns e a n p ace o errous u a e an so um . on a ns e
rema n ng macro- an m cronu r en s as escr e y uras ge oog .
Plant Tissue Culture Tested
orage emp
o u e n
-
a er
M510 MURASHIGE MODIFIED GERBERA PRETRANSPLANT BASAL MEDIUM
on a ns e a n p ace o errous u a e an so um . on a ns e
rema n ng macro- an m cronu r en s as escr e y uras ge oog .
an ssue u ure es e
Storage Temp
o u e n
2-6C
a er
1 L
H435 HOSTA INITIATION/ MULTIPLICATION MEDIUM
Stage I/II Medium
Modied Murashige & Skoog (1962) with (mg/L): 2.0 BA, 160 Adenine Hemisulfate, 500
ase n y ro ysa e, an .
an ssue u ure es e
Storage Temp
Soluble In
2-6C
Water
1 L
10 L
H436 HOSTA MULTIPLICATION MEDIUM
Stage II Medium
Modied Murashige & Skoog (1962) with (mg/L): 0.1 BA, 160 Adenine Hemisulfate, 500
ase n y ro ysa e, an .
an ssue u ure es e
Storage Temp
Soluble In
2-6C
Water
1 L
10 L
50 L
H437 HOSTA ROOTING MEDIUM
Stage III Medium
Modied Murashige & Skoog (1962) with (mg/L): 0.1 BA, 500 Casein Hydrolysate, and 0.5
an ssue u ure es e
Storage Temp
Soluble In
2-6C
Water
1 L
10 L
50 L
8/12/2019 Plant TC Media
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6
All components
expressed in mg/L
Murashig
eModiedAfricanViolet/
GloxiniaM
ultiplicationBasalMediu
urasge
o
ercan
oe
GloxiniaPretransplantBasalMediu
o
ee
u
ra
opss
Mura
shigeModiedFern
up
caon
asa
e
u
MurashigeModiedGerbera
MultiplicationBasalMediu
uras
ge
o
eerera
reranspan
asa
e
u
HostaInitiation/Multiplication
e
u
Hosta
MultiplicationMediu
Hos
taRootingMediu
urasge
oog
asa
e
um
us
eormua
eerence
COMPONENT 517 518 550 508 M509 510 435 436 437 519
Ammonium Nitrate
Boric Acid . . . . . . . . . .
a c u m or e, n y rous . . . . .
o a or e2
. . . . . . . . . .
Cupric Sulfate2O . . . . . . . . . .
Na2EDTA2H
2O . . . . .
Ferric Sodium EDTA . . . . .
errous u a e2
. . . . .
agnesum u a e, n y rous . . . . .
Manganese SulfateH2O . . . . . . . . . .
Molybdic Acid (Sodium Salt)2H2O . . . . . . . . . .
o ass um o e . . . . . . . . . .
o ass um ra e
o ass um osp a e, ono asc
Sodium Phosphate Monobasic
Zinc Sulfate7H2O . . . . . . . . . .
enne em su a e
gar
6-Benzylaminopurine (BA) . . .
Casein, Enzymatic Hydrolysate
Glycine (Free Base) . . .
, - c orop enoxyace c c .
n o e- -ace c c . . .
n o e- - u yr c c
Kinetin . . .
MES (Free Acid)
myo- nos o
- ap a eneace c c . . . .
Nicotinic Acid (Free Acid) . .
Peptone from Meat
PyridoxineHCI . . . .
ucrose
am ne . . . . . . .
L-Tyrosine
Grams of powder to prepare 1 liter . . . . . . . . . .
pH0.5 at RT . . . . . . . . .
= o pec ca on s a s e ev
8/12/2019 Plant TC Media
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7
MS-Based Plant-Specifc Media (continued)
Product
NumberProduct Description Product Notes
Package
Size
on a ns e a n p ace o errous u a e an so um . on a ns e
rema n ng macro- an m cronu r en s as escr e y uras ge oog .
Plant Tissue Culture Tested
orage emp
o u e n
-
a er
M512 MURASHIGE MODIFIED KALANCHOE PRETRANSPLANT BASAL MEDIUMContains FeNaEDTA in place of Ferrous Sulfate and Disodium EDTA. Contains the
rema n ng macro- an m cronu r en s as escr e y uras ge oog .
an ssue u ure es e
Storage TempSoluble In
2-6Ca er
1 L
M513 MURASHIGE MODIFIED LILY MULTIPLICATION BASAL MEDIUM
Contains FeNaEDTA in place of Ferrous Sulfate and Disodium EDTA. Contains the
remaining macro- and micronutrients as described by Murashige & Skoog (1962).
Plant Tissue Culture Tested
Storage Temp
Soluble In
2-6C
Water
1 L
10 L
50 L
IITA Formulation as described by Vuylsteke (1998).
Plant Tissue Culture Tested
orage emp
Soluble In
-
Water
10 L
50 L
Contains FeNaEDTA in place of Ferrous Sulfate and Disodium EDTA. Contains the
remaining macro- and micronutrients as described by Murashige & Skoog (1962).
Plant Tissue Culture Tested
orage emp
Soluble In
-
Water
10 L
50 L
All components
expressed in mg/L uras
ge
o
e
aancoe
Mult-
plication
Basal
e
u
Murashige
Modied
aancoe
re-
ranspan
Basal
Mediu
uras
ge
o
e
LilyMulti-
plication
Basal
e
u
Musa
(Banana)
u
-
pcaon
e
uMS
o
e
oao
asa
Mediu
OMPONENT 511 512 M513 462 516
Ammonium Nitrate
Boric Acid . . . . .
Calcium Chloride, Anhydrous .
Cobalt Chloride6H . . . . .
Cupric Sulfate5H O . . . . .
a O .
erric Sodium EDTA . . . .
errous Sulfate7H2O .
Magnesium Sulfate, Anhydrous .
Manganese SulfateH2
. . . . .
Molybdic Acid (Sodium Salt)2H2O . . . . .
Potassium Iodide . . . . .
Potassium Nitrate
Potassium Phosphate, Monobasic
Zinc Sulfate7H2O . . . . .
L-Ascorbic Acid
6-Benzylaminopurine (BA) .
Gelrite
D-Glucose 2.0
Glycine (Free Base) 2.0 2.0
6- , -Dimethylallylaminopurine (2iP) .0
ndole-3-acetic Acid .0 .175
Kinetin .04
myo-Inositol 100 100 100 100
-Naphthaleneacetic Acid 0.03
Nicotinic Acid (Free Acid) 0.5 .5
PyridoxineHCI 0.5 .5
Sucrose 30,000
ThiamineHCI .4 .4 0.4 0.4 .4
Grams of powder to prepare 1 liter 4.41 .41 4.4 6.36 .41
pH0.5 at RT 4.8 4.8 4.8 4.3 S
NS = No Specication Established
8/12/2019 Plant TC Media
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8
Vitamins
Product
NumberProduct Description Product Notes
Package
Size
C149 CHU N6 VITAMIN SOLUTION (1000x)
on a ns e v am ns as escr e y u .
an ssue u ure es e
Storage Temp
o u e n
- C
a er
m
E330 ERIKSSON VITAMIN SOLUTION (1000x)
Contains the vitamins as described by Eriksson (1965).Plant Tissue Culture Tested
Storage Temp
Soluble In
2-6C
Water
100 mL
x
on a ns e v am ns as escr e y am org, e a . .
Plant Tissue Culture Tested
orage emp
o u e n
-
a er
m
G219 GAMBORG VITAMIN SOLUTION (1000x)
on a ns e v am ns as escr e y am org, e a . .
an ssue u ure es e
Storage Temp
o u e n
- C
a er
m
K421 KAO & MICHAYLUK VITAMIN SOLUTION (100x)
Contains the vitamins described by Kao & Michayluk (1975).
Plant Tissue Culture Tested
Storage Temp
Soluble In
2-6C
Water
100 mL
500 mL
x
on a ns e v am ns as escr e y uras ge oog .
Plant Tissue Culture Tested
orage emp
o u e n
-
a er
m
m
MURASHIGE & SKOOG VITAMIN SOLUTION (1000x)
on a ns e v am ns as escr e y uras ge oog .
an ssue u ure es e
Storage Temp
o u e n
- C
a er
m
M547 MURASHIGE & SKOOG MODIFIED VITAMIN POWDER (1000x)
Murashige & Skoog (1962) vitamins modied with additional ThiamineHCl.
Plant Tissue Culture Tested
100 mL
250 mL
x
Murashige & Skoog (1962) vitamins modied with additional ThiamineHCl.
Plant Tissue Culture Tested
orage emp
Soluble In
-
Water
m
NITSCH & NITSCH VITAMIN POWDER (1000x)
on a ns e v am ns as escr e y sc sc .
an ssue u ure es e
Storage Temp
o u e n
- C
a er
m
m
N603 NITSCH & NITSCH VITAMIN SOLUTION (1000x)
Contains the vitamins as described by Nitsch & Nitsch (1969).
Plant Tissue Culture Tested
Storage Temp
Soluble In
2-6C
Water
100 mL
x
Contains the vitamins as described by Schenk & Hildebrandt (1972).
Plant Tissue Culture Tested
orage emp
Soluble In
-
Water
m
1 L
All components
expressed in mg/LChuN6Vitamin
Solution(1000x)
ErikssonVitamin
Solution(1000x)
GamborgVitamin
Powder(1000x)
GamborgVitamin
Solution(1000x)
Kao&Michayluk
VitaminSolution
(100x)
MSVitamin
Powder(1000x)
MSVitamin
Solution(1000x)
MSModied
VitaminPowder
(1000x)
MSModied
VitaminSolution
(1000x)
Nitsch&Nitsch
VitaminPowder
(1000x)
Nitsch&Nitsch
VitaminSolution
(1000x)
Schenk
Hildebrandt
VitaminPowder
(100x)
p- m no enzo c c .
L-Ascorbic Acid
D-Biotin .
- a c um an o ena e
Choline Chloride
yanco a am n,12
.
o c c . .
Glycine (Free Base)
myo-Inositol
co nam e
co n c c ree c
yr ox ne HCl .
Riboavin .
Thiamine HCI