Playing cards for Memory and Total recall

Pages 2 to 4: keyword-cards

Pages 5 to 7: keyword-cards with corresponding explanation-cards

Restriction Restriction Star fragment endonuclease activity Isoschizo- Gel

mer electrophoresis Slot

Growth Ethidium Electrophoretic

rate bromide mobility

Double Origin of Selectable digestion replication marker

pBR 322 MCS Polylinker

Complex -Galacto- High copy media sidase plasmid

Low copy Defined DNA plasmid media ligases Competent Escherichia pUC 19 cells coli Electro- Ligation OD poration

EcoR I lac-Operon Plasmid

Cloning Electropho- Base vector resis pair

Antibiotic Blunt end Sticky end resistance enzymes enzymes Restriction Endo- map nucleases Palindrome

DNA Recognition Target band site sequence Restriction Compatible enzyme Ampicillin ends

Electropho- Restriction Salt resis marker buffer competent lacZ gene ORI Heat shock Bromo- Xylen- Type I phenol blue xyanol enzyme

Hybridi- Probe Label sation Denatura- Southern RBS tion Blot Target Promoter Terminator sequence Expression Southern Western vector Blot Blot Agarose PCR Primer

Annealing Synthesis Cycle

Cassette

DNA modifying enzymes that recognise specific motifs of double stranded DNA and cleave it into fragments of different lengths

type II restriction enzyme: recognises a specific palindrome sequence motif (GAATTC)

sequence motif with an axis of rotational symmetry, the sequence in 5`3`-direction is equal on the both strands

small rings of double stranded extrachromosomal and independently reproduced DNA, natural types are important for antibiotic resistance and sexual reproduction of bacteria

vehicle of genetic engineering

shuttle that allows reintroducing of foreign DNA into a host organism

Endonucleases

Palindrome

Plasmids

EcoRI

Vector

bacterial plasmids with features for reintroducing of small foreign DNA-fragments into host organisms with the purpose of producing significant amounts of recombinant DNA

Cloning vector

diagram showing sites of cleavage of a DNA molecule, for example of a plasmid vector

Restriction map

Cleavage of DNA with this type of endonucleases creates a short complementary single stranded overhang in each cleaved end

Sticky end enzymes

Blunt end enzymes

Endonucleases that form no 5’or 3’ overhangs after cleavage

Competent cellsBacterial cells which are able to incorporate foreign DNA

lac-OperonBacterial expression unit of genes for lactose utilisation

Agarose gel electrophoresis

Method that separates DNA fragments in an electric field as a function of their lengths

PolylinkerStaged recognition sites of different restriction enzymes, a feature of vectors for variable insertion of foreign DNA

IsoschizomerRestriction enzymes that recognise the same target sequence

Ethidium bromide (EtBr)

Stain that intercalates into double-stranded DNA. Under UV-light the complex of DNA and the stain fluoresces pink