Portulaca Extract-TLSalfakemindo.co.id/wp-content/uploads/2019/09/Portulaca...Portulaca Extract-TLS has been shown to inhibit NO and Lipoxygenase, which play an important role in inflammatory

the one who knows natural productsPORTULACA EXTRACT-TLS

Head Office | #12-24, Gajangsaneopseo-ro, Osan-si, Gyeonggi-do, Korea | Tel: +82.31.374.5240 Fax: +82.31.374.5246

www.natrualsolution.co.kr [email protected]

PORTULACA EXTRACT-TLS

Natural Choice for Dry and Sensitive Skin

Product for anti-allergy and anti-irritation


PLANT STORY

Portulaca oleracea is an annual succulent with bright yellow

flowers surrounded by a cluster of leaves. The plant is known

as purslane, little hogweed or pusley and Machixian in China

and Machihyun in Korea meaning ‘horse teeth amaranth’ due

to its resemblance to horse’s teeth. It is an edible nutritious

vegetable, distributed all over the world and generally used in

Korean and Chinese traditional medicines.


PLANT STORY

In oriental herbal medicine, purslane is used to remove

toxic heat and substances, to arrest bleeding and to treat

infections and eczema. It is one of the richest green plant

sources of omega-3 fatty acids. The recent scientific

research showed that P. oleracea exhibited a wide range

of biological activity including anti-inflammatory, anti-

oxidant and wound healing activities.


THE COMPOSITION OF PORTULACA OLERACEA

Polysaccharides: Portulaca oleracea contains 9-14% polysaccharides.

Amino acids: glutamine acid, aspartic acid, alanine, and etc.

Vitamins: vitamin A, vitamin C, vitamin B and carotenoids

Minerals: magnesium, calcium, potassium and iron

Yellow betaxanthins and reddish betacyanins

NMF (Natural Moisturizing Factor) Compounds

Vitamins & Minerals

Antioxidants


IN VITRO EFFICACY EVALUATOIN

Anti-allergic Activity: ß -hexosaminidase inhibition

Anti-inflammatory Activity:

NO Synthesis Inhibition

5-lipoxygenase Inhibition

Wound Healing Activity


ANTI-ALLERGIC RESPONSE

An allergic reaction takes place when the immune system of an

animal or human responds to the exposure of a variety of

substances called allergens.

Histamine and ß-hexosaminidase are considered to be

markers of mast cell degranulation, mediators of inflammatory

responses. Thus, the ß -hexosaminidase activity in the medium

is used as a marker of mast cell degranulation. The inhibition

of ß -hexosaminidase release is required for an

antiallergenic response.


IN VITRO EVALUATION: ANTI-ALLERGY

- BETA-HEXOSAMINIDASE RELEASE INHIBITION

0

20

40

60

80

100

120

Control 10 50 100

% o

f C

on

tro

l

Concentration (ppm)

β-hexosaminidase

Viability


WHAT IS INFLAMMATION?

Inflammation is part of the complex biological responses to wide range

of harmful stimuli including injury, tissue necrosis, infection, and

irritants. The purpose of inflammation is to destroy (or contain) the

damaging agent, initiate repair processes and return the damaged

tissue to useful function. The symptoms of inflammation are redness,

swelling, heat, and pain, which are caused by increased blood flow

into tissue. The immune system is responsible of protecting our body

from the harmful stimuli and of maintaining homeostasis. Disorders of

the immune system can result in autoimmune diseases, inflammatory

diseases, and cancer. In an attempt to protect the body, the immune

system might overreact to the stimuli, and this might cause allergy or

inflammatory reactions.


INFLAMMATION MECHANISM

PortulacaExtract-TLS

A variety of enzymes are secreted when cells on the skin tissue are stimulated by stimulus.

Nitric oxide is an important molecule in cell signaling and plays an important role in biological processes. Small amounts of nitric oxide help cell

activity, but as the amount and the reaction time become longer, intracellular COX-2 and LOX-5 levels are increased. Portulaca Extract-TLS has

been shown to inhibit NO and Lipoxygenase, which play an important role in inflammatory signals, and it is helps relieve and improve inflammation

reaction.

InflammatoryStimulus

Membrane Phospholipids

Arachidonic Acid

Inflammation

COX-2Cyclooxygenase

PGE, PGI, PGD

LOX-5Lipoxygenases

HPETEs, HETEs, Leukotrienes,

Lipoxins

PortulacaExtract-TLS

Inhibitory inflammation


IN VITRO EVALUATION: ANTI-INFLAMMATION

Inhibition of NO Production Lipoxygenase Inhibition Activity

0

10

20

30

40

50

60

70

0.3 0.75 1.25

LO

In

hib

itio

n A

cti

vit

y (

%)

Concentration (%, v/v)

0

20

40

60

80

100

120

Control 10 50 100

% o

f C

on

tro

l

Concentration (ppm)

NO

Viability


WOUND HEALING ASSAY

Scratch Wound Healing Assay has been widely adapted and

modified by researchers to study the effects of a variety of

experimental conditions, for instance, gene-knockdown or

chemical compound treatment, on cell migration and

proliferation.

The basic principle of the assay is that, a “wound gap” in a cell

monolayer is created by scratch, followed by monitoring the

“healing” of this gap by cell migrating and growth towards the

center of the gap, hereby filling up the “gap”. Factors that alter

the motility and/or growth of the cell can lead to increased or

decreased rate of “healing” of the gap.


IN VITRO EVALUATION: WOUND HEALING

Day 0 Day 3 Day 7

After 24 hours of culture, cells were scratched with a 200 μL pipette tip and cultured for additional 48 hours. Photos for the

stained monolayer were taken on a microscope.

Control

Portulaca Extract


IN VIVO EVALUATION

Skin Soothing Activity


IN VIVO EVALUATION: SKIN SOOTHING

0 1 2 3 4

5 6 7 8 9

• Target site: Forearm

• Subjects: 10 females, aged between 18 to 65 years old

(Average age: 42.40±7.95)

• Test item: Cream with 2% Portulaca Extract-TLS

3% SLS solution patches to induce erythema

• Application: 40 mg of cream on application area

• Application area: 20 cm2/site (4 cm * 5 cm)

• Measurements: 0, 1, 3, 5, 7, 9, 10 days after application

• Test instrument: Mexameter MX18 (CK Electronic GmbH, Germany)

• Scoring reference for dermatologist’s evaluation:

• Score 0 : No erythema

• Score 1-3 : Slight erythema

• Score 4-6 : Moderate erthema

• Score 7-9 : Severe erythema

Application area



- DERMATOLOGIST’S ERYTHEMA SCOREOn Day 0, patches containing 3% of SLS solution were applied for 24 hours to induce erythema onto the forearms of

10 female volunteers aged between 18 and 65. The erythema was evaluated by dermatologist and by Mexameter

every 2 days. 40 mg of test product and placebo were applied on forearms.

0

1

2

3

4

5

6

SLS Day 1 Day 3 Day 5 Day 7 Day 9 SLS Day 10

Derm

ato

log

ist'

s S

co

rin

g

Time (Day)

Placebo

Portulaca Extract-TLS

24h

occlu

siv

e S

LS

tre

atm

ent

24h

occlu

siv

e S

LS

tre

atm

ent



- ERYTHEMA VALUE

0

50

100

150

200

250

300

SLS Day 1 Day 3 Day 5 Day 7 Day 9 SLS Day 10

E V

alu

e

Time (Day)

Placebo

Portulaca Extract-TLS

24h

occlu

siv

e S

LS

tre

atm

ent

24h

occlu

siv

e S

LS

tre

atm

ent

On Day 0, patches containing 3% of SLS solution were applied for 24 hours to induce erythema onto the forearms of

10 female volunteers aged between 18 and 65. The erythema was evaluated by dermatologist and by Mexameter

every 2 days. 40 mg of test product and placebo were applied on forearms.


MARKETING POINT

Anti-allergy and anti-inflammatory effects

Skin soothing and recovery

Reduce skin erythema and relieve the irritation

Highly recommended for dry and sensitive skin, such as atopic dermatitis

and eczema


PRODUCT INFORMATION

Product Name: Portulaca Extract-TLS, Portulaca Extract-TLS(PD)

INCI Name: Portulaca Oleracea Extract

Dosage: 1 – 3%

Formulation: Add to the formulation when the temperature is lower than 55°C. Recommended to add after the cooling process.

Storage: Avoid direct light or UV. Keep it in a cool and dry area.

the one who knows natural productsPORTULACA EXTRACT-TLSThe Garden of Naturalsolution, the one who knows natural products

website.

email.

www.naturalsolution.co.kr

[email protected]

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Portulaca Extract-TLSalfakemindo.co.id/wp-content/uploads/2019/09/Portulaca...Portulaca Extract-TLS has been shown to inhibit NO and Lipoxygenase, which play an important role in inflammatory