Preliminary Detailed Design Review

P15080Flow Culture System November 13, 2014

Engineering Requirements● No changes have been made since last

meeting● Document is under change control● Most important ERs are:

o Shear Stresso Assembly Timeo Size of Flow Channel

EDGE Link

System Model/Architecture/Design

Growth chamber design changes:o Minor updates to system architecture

diagram and interfaces documento Generated new 3-D model of growth chambero Verified chamber can be integrated into

systemo Verifying lid thickness and attachment

mechanism is appropriate

Chamber/System Integration Link

Pump

Cell Chamber

Media Reservoir

Electricity

Flow Rate

Cells

MicroscopesAlternate Reservoir

Media

Media Color

HeatCO2Water Pan

Architecture Color

Structure

Energy

Information

Materials

Incubator

Filter

System Architecture

Media in Tubing

Media in Tubing

Media in Tubing

Light

CO2

(moves while still attached)

Pump Setting

(swap)

(pumped into

incubator)

Heating Element

Power Cord

Power Cord

Power Cord

Fresh Media

Fresh Media

(seeded) Microscope Slide

Lid

(in a groove with a gasket)Base

4 Bolts and 4 Wing Nuts(lid has

gasket)

Growth Chamber

Wing Nuts

Lid Cap

Metric Microscope Slides

Lid and Slide Gaskets

Body

Base Cap

Bolts

● Aluminum 6061 10” x 2.5” x 0.5” body sandwiched by two layers of 0.25” polycarbonate

● 1 mm x 1 mm rubber gasket fits around channel, bolts keep lid pressed down to seal top of channel

● 1 mm x 2 mm rubber gasket fits around either slide recession, tight fit of slides maintains a seal on bottom of channel

● Four 0.25” bolts run through corners of chamber and keep the system together, used with wing nuts to be tightened manually

Growth Chamber Connections

Threaded Inlet/Outlet Holes

Threaded Luer

Adapter

Male Luer Adapter

3-Way Stopcock

Female Luer

Adapter

● All components are polycarbonate and are autoclavable● Threaded Luer adapters fit into the chamber inlet and outlet holes● The threaded Luer adapters connect to a male or female Luer adapter each (both

are compatible)● A short section of tubing will be attached next● A female or male Luer adapter connects to both open tubing ends● 3-way stopcocks connect to the Luer adapters, oriented as needed (to

accommodate a male or female adapter)● A second 3-way stopcock will be added to each side● The appropriate Luer adapter (opposite of the last type used) connects to the open

stopcocks● The system’s inlet and outlet tubes can now connect to these adapters and connect

the growth chamber

Feasibility Analysis: Lid Deflection

Feasibility Analysis: Flow

Required to achieve 2 Pa of shear stress on

cells.

Q = 640 mL/min

Feasibility Analysis: Flow

Pressure Drop is

approximately

582 Pa

Feasibility Analysis: Flow

Conclusion:Design is feasible!

● Reaches 2 Pa requirement

Pump requirements confirmed.

● Flow range● Tubing Size

CAD Drawing

CAD Drawing

CAD Drawing

CAD Drawing

Risk Analysis ● Risk is decreasing nicely● Largest Risks

○ Control of pump○ Sterility○ Incubator/Pump

Interface● All risks that had a

deadline of 11/13/14 to be driven to zero are zero

● Linked parts on BOM to Risk Analysis

● Linked risks to feasibility models○ Some feasibility still

in progress● Linked risks to Test Plans

Project Plan

To Do:● Deflection Discussion with SME

o Develop COMSOL deflection model and verify Analytical Model

● Final Drawingso Draft Final Drawingso Brinkman evaluation of tolerances/design and

quote● Quotes and Purchase Orders and Lead Times

o Tubing, Luers, Stopcocks● Begin Pump Testing

o Locate Tubing within BME Dept.

Critical Path Into Phase 5

Efficiency● Average Efficiency Last Phase:70%● Average Efficiency This Phase: 89%

Overall efficiency has improved this phaseo Realistic Deadlineso Better communication

● Ways to Improve Furthero Consider other coursework when setting

deadlines

HW/SW DesignThis is not applicable to our project.

Initial Budget and BOM● Ordered Pump● Total Projected Budget is $957.65

o Need to request quoteso Need to finalize shipping costs and discounts

● Parts linked to risks● Part numbers linked to design

drawings

Link

Part-Linked Media System

Part-Linked Growth Chamber

Part-Linked Complete System

Not Shown:● Incubato

r● Cells● Gaskets● Nuts● Bolts

Assembly Process● Previously completed a preliminary

assembly procedure● Revisions to account for growth

chamber design changes● Likely will be adjusted during

construction in MSD II

Test Plan Outline● Added two test plans

o Tubing in Incubator Dooro Blockage of Flow-Ajar Microscopes

● Plan to add more as ideas come and materials arrive

Link

Self Assessment● Important to have accountability

partnerso Have others double check work!

● Important to ask for other group members opinions

● Recognize costs - they add up quickly!● Balance with other coursework