Presented by Mohammed A. Alyami Head Teaching Assistant Department of Pharmacology & Toxicology...
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Introduction and CNS stimulants Presented by Mohammed A. Alyami Head Teaching Assistant Department of Pharmacology & Toxicology College of Pharmacy KSU E-mail : [email protected]
Presented by Mohammed A. Alyami Head Teaching Assistant Department of Pharmacology & Toxicology College of Pharmacy KSU
Presented by Mohammed A. Alyami Head Teaching Assistant
Department of Pharmacology & Toxicology College of Pharmacy
KSU
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All Courses of Pharmacology Lab animals Restraint Injection
Instructions marks
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PHL 313 Pharmacology-1 - PNS & CVS PHL 313 Pharmacology-1 -
PNS & CVS PHL 322 Pharmacology-2 - CNS & Psychiatric PHL
418 Pharmacology-3 - Anti-lpidemics Anticoagulants Antimalarial PHL
418 Pharmacology-3 - Anti-lpidemics Anticoagulants Antimalarial PHL
425 Pharmacology-4 Anticancer And hormones and skin treatment PHL
425 Pharmacology-4 Anticancer And hormones and skin treatment LAB
NO LAB All pharmacology courses
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Lab Animals will be used in PHL 322 Mouse Rat Frog
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Restraint of mouse A.Tail Restraint Used for : transferring
animals from cage to cage. Method : Mice may be picked up by
grasping the base of the tail. Note: Do not grasp the tip of the
tail, as this may cause the skin to be stripped off.
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B. Scruff Restraint Used for : Injections or blood withdrawal.
Method : 1- Restrain the mouse by grasping it near the base of the
tail and placing it on a toe-gripping surface, such as a wire bar
lid. Pulling back gently on the tail of the mouse causes it to pull
forward on the toe-gripping surface. Restraint of mouse
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2- While grasping the tail with one hand, grasp the nape or
scruff of the neck with the other. Restraint of mouse
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3- Position the animals body firmly across your hand by
extending your forefinger and thumb back as far as possible, while
maintaining a firm grip on the scruff. Place the tail between the
fingers of this same hand to secure the animal. ** If you do not
grasp enough of the scruff, the animal will be able to turn and
bite. ** If you grasp too much skin, the airway will become
restricted and the mouse will become cyanotic This type of
restraint will allow the handler complete access to the ventral
side of the mouse. Restraint of mouse
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Injection I ntraperitoneal Injections ( IP ) - Restrain the
mouse by the scruff method. -E-Expose the ventral side of the
animal, tilting the head down at a slight angle. -T-The sterile
needle should be placed, bevel up, in the lower right or left
quadrant of the animals abdomen. -I-Insert the needle at a 30
angle.
Total marks = 100 Theoretical = 70 Practical = 30 Reports = 5
Tutorial = 10 Exam of work lab = 10 Exam of theoretical lab = 5
Last lab is very important Each lab you will have report for work
lab only
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Reports PHL 322 Lab # ( ) Title .. Name : .. ID NO. . Day