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Principles of Chromatography
Process by which one separate compounds from one another by passing a mixture through a column that retains some compounds longer than others.
Chromatography
Column Eluent
In
Eluate
Out
Mobile phase = solvent moving through the column. Stationary phase = substance that stays fixed inside the column. Eluent = fluid entering the column. Eluate = fluid exiting the column.
Gas Chromatography = gas carrier Liquid Chromatography = liquid mobile phase
Gas Chromatography
Column separation (gas-liquid, gas-solid) used for separating and analyzing compounds that can be vaporized without decomposition.
Separations based on differences in boiling points!
Liquid Chromatography
Column separation (liquid-liquid, liquid-solid) used for separating and analyzing compounds based on differences in their interaction with a stationary phase.
Adsorption, partition, ion exchange, molecular exclusion and affinity
Efficiency of Separation
Multiple Flow Path Term
H = L/N Small plate height = narrow peaks = better separation
Zone Broadening Terms
A term = Eddy Diffusion
Bterm = Longitudinal Diffusion
C term = Stationary Phase Mass Transport