Publications for High-Content Screening

OPERETTA CLS

Cabel, C. R.; Alizadeh, E.; Robbins, D. J.; Ahmed, Y.; Lee, E.; and Thorne, C. A. (2019). Single-Cell Analyses Confirm the Critical Role of LRP6 for Wnt Signaling in APC-Deficient Cells. Developmental Cell, 49(6), 827–828. DOI: 10.1016/j.devcel.2019.05.039

Cox, A.; Vinciguerra, D.; Re, F.; Magro, R. D.; Mura, S.; Masserini, M.; Nicolas, J. (2019). Protein-functionalized nanoparticles derived from end-functional polymers and polymer prodrugs for crossing the blood-brain barrier. European Journal of Pharmaceutics and Biopharmaceutics, 142, 70–82. DOI: 10.1016/j.ejpb.2019.06.004

Fujikawa, Y.; Terakado, K.; Nampo, T.; Mori, M.; and Inoue, H. (2019). 4-Bromo-1,8-naphthalimide derivatives as fluorogenic substrates for live cell imaging of glutathione S-transferase (GST) activity. Talanta, 204, 633–640. DOI: 10.1016/j.talanta.2019.06.059

Handorf, O.; Schnabel, U.; Bösel, A.; Weihe, T.; Bekeschus, S.; Graf, A. C.; Ehlbeck, J. (2019). Antimicrobial effects of microwave-induced plasma torch (MiniMIP) treatment on Candida albicans biofilms. Microbial Biotechnology, 12(5), 1034–1048. DOI: 10.1111/1751-7915.13459

Khabipov, A.; Käding, A.; Liedtke, K. R.; Freund, E.; Partecke, L.-I.; and Bekeschus, S. (2019). RAW 264.7 Macrophage Polarization by Pancreatic Cancer Cells – A Model for Studying Tumour-promoting Macrophages. Anticancer Research, 39(6), 2871–2882. DOI: 10.21873/anticanres.13416

Kong, L.; Wu, Z.; Zhao, Y.; Lu, X.; Shi, H.; Liu, S.; and Li, J. (2019). Qigesan reduces the motility of esophageal cancer cells via inhibiting Gas6/Axl and NF-kB expression. Bioscience Reports, 39(6). DOI: 10.1042/BSR20190850

Li, M.; Lee, A.; Kim, S.; Shrinidhi, A.; Min Park, K.; and Kim, K. (2019). Cucurbit[7]uril-conjugated dyes as live cell imaging probes: investigation on their cellular uptake and excretion pathways. Organic and Biomolecular Chemistry, 17(25), 6215–6220. DOI: 10.1039/C9OB00356H

Publications in which PerkinElmer high-content analysis and screening systems are used number more than 1000 between 2010 and 2019. Here we feature some of the more recent publications, focussing on Operetta CLS and Opera Phenix, and categorized by application or research area.

Table of Contents1. Operetta CLS High-Content

Analysis System 2. Opera Phenix High-Content

Screening System 3. Cancer Research 4. Viral Disease

5. Bacterial Disease 6. Parasitic Disease 7. Neuroscience 8. Toxicology 9. Stem Cells

10. 3D Cell Models and Cell Cultures

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Ng, C. L.; and Fidock, D. A. (2019). Plasmodium falciparum In Vitro Drug Resistance Selections and Gene Editing. In F. Ariey, F. Gay, and R. Ménard (Eds.), Malaria Control and Elimination (pp. 123–140). DOI: 10.1007/978-1-4939-9550-9_9

Onyema, O. O.; Guo, Y.; Mahgoub, B.; Wang, Q.; Manafi, A.; Mei, Z.; Krupnick, A. S. (2019). Eosinophils downregulate lung alloimmunity by decreasing TCR signal transduction. JCI Insight, 4(11). DOI: 10.1172/jci.insight.128241

Pan, C.; Onda, K.; and Hirano, T. (2019). Bevacizumab Versus Anti-preeclamptic Drugs: Evaluation With Three-dimensionally Co-cultured Human Mini Tumors. Anticancer Research, 39(7), 3543–3551. DOI: 10.21873/anticanres.13500

Xu, Y.; Lin, J.; Jin, Y.; Chen, M.; Zheng, H.; and Feng, J. (2019). The miRNA hsa-miR-6515-3p potentially contributes to lncRNA H19-mediated-lung cancer metastasis. Journal of Cellular Biochemistry. DOI: 10.1002/jcb.29006

OPERA PHENIX HIGH-CONTENT SCREENING SYSTEM

Aguirre, AJ.; Meyers, RM.; Weir, BA.; Vazquez, F.; Zhang, CZ.; Ben-David, U.; Cook, A.; Ha, G.; Harrington, WF.; Doshi, MB.; Kost-Alimova, M.; Gill, S.; Xu, H.; Ali, LD.; Jiang, G.; Pantel, S.; Lee, Y.; Goodale, A.; Cherniack, AD.; Oh, C.; Kryukov, G.; Cowley, GS.; Garraway, LA.; Stegmaier, K.; Roberts, CW.; Golub, TR.; Meyerson, M.; Root, DE.; Tsherniak, A.; and Hahn, WC. (2016) Genomic copy number dictates a gene-independent cell response to CRISPR-Cas9 targeting. Cancer Discovery. DOI: 10.1158/2159-8290.CD-16-0154

Barrows, NJ.; Campos, RK.; Powell, ST.; Prasanth, KR. A Screen of FDA-Approved Drugs for Inhibitors of Zika Virus Infection. Cell Host and Microbe (2016), DOI: 10.1016/j.chom.2016.07.004

Booth, H. D. E.; Wessely, F.; Connor-Robson, N.; Rinaldi, F.; Vowles, J.; Browne, C.; Wade-Martins, R. (2019). RNA sequencing reveals MMP2 and TGFB1 downregulation in LRRK2 G2019S Parkinson’s iPSC-derived astrocytes. Neurobiology of Disease, 129, 56–66. DOI: 10.1016/j.nbd.2019.05.006

Christoffersson, J.; Meier, F.; Kempf, H.; Schwanke, K.; Coffee, M.; Beilmann, M.; Mandenius, C.-F. (2019). Evaluating the Effect of Drug Compounds on Cardiac Spheroids Using the Cardiac Cell Outgrowth Assay. In C.-F. Mandenius and J. A. Ross (Eds.), Cell-Based Assays Using iPSCs for Drug Development and Testing (pp. 185–193). DOI: 10.1007/978-1-4939-9477-9_17

Conos, SA.; Lawlor, KE.; Vaux, DL.; Vince, JE. Cell death is not essential for caspase-1-mediated interleukin-1 activation and secretion. Cell Death and Differentiation (2016), DOI: 10.1038/cdd.2016.69

Dawe, G. B.; Yu, H.; Gu, S.; Blackler, A. N.; Matta, J. A.; Siuda, E. R.; Bredt, D. S. (2019). 7 nicotinic acetylcholine receptor upregulation by anti-apoptotic Bcl-2 proteins. Nature Communications, 10(1), 1–11. DOI: 10.1038/s41467-019-10723-x

Dent, M. P.; Madnick, S. J.; Hall, S.; Vantangoli Policelli, M.; Bars, C.; Li, H.; Boekelheide, K. (2019). A human-derived prostate co-culture microtissue model using epithelial (RWPE-1) and stromal (WPMY-1) cell lines. Toxicology in Vitro, 60, 203–211. DOI: 10.1016/j.tiv.2019.05.023

Fang, M. Y.; Markmiller, S.; Vu, A. Q.; Javaherian, A.; Dowdle, W. E.; Jolivet, P.; Yeo, G. W. (2019). Small-Molecule Modulation of TDP-43 Recruitment to Stress Granules Prevents Persistent TDP-43 Accumulation in ALS/FTD. Neuron. DOI: 10.1016/j.neuron.2019.05.048

Fraietta, I. and Gasparri, F. (2016_ The development of high-content screening (HCS) technology and its importance to drug discovery. Expert Opinion on Drug Discovery, Vol.11, 5. DOI: 10.1517/17460441.2016.1165203

Guo, SM.; Veneziano, R.; Gordonov, S.; Li, L.; Park, D.; Kulesa, AB.; Blainey, PC.; Cottrell, JR.; Boyden, ES.; and Bathe, M. (2017) Multiplexed confocal and super-resolution fluorescence imaging of cytoskeletal and neuronal synapse proteins. bioRxiv.

Jang, I.; Pottekat, A.; Poothong, J.; Yong, J.; Lagunas-Acosta, J.; Charbono, A.; Kaufman, R. J. (2019). PDIA1/P4HB is required for efficient proinsulin maturation and β cell health in response to diet induced obesity. ELife, 8. DOI: 10.7554/eLife.44528

Kleensang, A.; Vantangoli, MM.; Odwin-DaCosta, S. (2016) Genetic variability in a frozen batch of MCF-7 cells invisible in routine authentication affecting cell function. Scientific Reports 6(28994). DOI: 10.1038/srep28994

Kokubu, Y.; Nagino, T.; Sasa, K.; Oikawa, T.; Miyake, K.; Kume, A.; Sakurai, H. (2019). Phenotypic Drug Screening for Dysferlinopathy Using Patient-Derived Induced Pluripotent Stem Cells. Stem Cells Translational Medicine. DOI: 10.1002/sctm.18-0280

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Kriston-Vizi, J.; and Flotow, H. (2016) Getting the whole picture: High content screening using three-dimensional cellular model systems and whole animal assays. Cytometry Part A. DOI: 10.1002/cyto.a.22907

Kriston-Vizi, J.; Harding, SE.; and Földes, G. Structural Toxicity: Hypertrophy Models of Human Pluripotent Stem Cell-Derived Cardiomyocytes. Stem Cell-Derived Models in Toxicology. DOI: 10.1007/978-1-4939- 6661-5_14

Letzsch, S.; Boettcher, K.; Kelm, JM.; and Messner, S. (2015) Quantifying Efflux Activity in 3D Liver Spheroids : New Confocal Imaging Instruments Allow Screening in Complex Human Liver Microtissues. Genetic Engineering and Biotechnology News, 35(7), 14-15. DOI: 10.1089/gen.35.07.08

Li, L.; Zhou, Q.; Voss, TC.; Quick, KL and LaBarbera, DV. (2016) High-throughput imaging: Focusing in on drug discovery in 3D. Methods 96, 97–102. DOI: 10.1016/j.ymeth.2015.11.013

Li, M.; Wang, X.; Cao, L.; Lin, Z.; Wei, M.; Fang, M and Li, S. (2016) Quantitative and epitope-specific antigenicity analysis of the human papillomavirus 6 capsid protein in aqueous solution or when adsorbed on particulate adjuvants. Vaccine 34(37), 4422-4428. DOI: 10.1016/j.vaccine.2016.07.012

Neumann, J.; Ziegler, K.; Gelléri, M.; Fröhlich-Nowoisky, J.; Liu, F.; Bellinghausen, I.; Lucas, K. (2019). Nanoscale distribution of TLR4 on primary human macrophages stimulated with LPS and ATI. Nanoscale, 11(19), 9769–9779. DOI: 10.1039/C9NR00943D

Phillips, SL.; Soderblom, EJ.; Bradrick, SS.; Garcia-Blanco, MA. (2016) Identification of proteins bound to dengue viral RNA in vivo reveals new host proteins important for virus replication. mBio 7(1):e01865-15. DOI: 10.1128/mBio.01865-15

Plys, A. J.; Davis, C. P.; Kim, J.; Rizki, G.; Keenen, M. M.; Marr, S. K.; and Kingston, R. E. (2019). Phase separation of Polycomb-repressive complex 1 is governed by a charged disordered region of CBX2. Genes and Development. DOI: 10.1101/gad.326488.119

Sun, Y.; Sun, Y.; Yan, K.; Li, Z.; Xu, C.; Geng, Y.; Xi, Q. (2019). Potent anti-tumor efficacy of palbociclib in treatment-naïve H3.3K27M-mutant diffuse intrinsic pontine glioma. EBioMedicine, 43, 171–179. DOI: 10.1016/j.ebiom.2019.04.043

Guo, SM.; Veneziano, R.; Gordonov, S.; Li, L.; Park, D.; Kulesa, AB.; Blainey, PC.; Cottrell, JR.; Boyden, ES.; and Bathe, M. (2017). Multiplexed confocal and super-resolution fluorescence imaging of cytoskeletal and neuronal synapse proteins. bioRxiv.

Verschuuren, M.; Verstraelen, P.; García-Díaz Barriga, G.; Cilissen, I.; Coninx, E.; Verslegers, M.; De Vos, W. H. (2019). High-throughput microscopy exposes a pharmacological window in which dual leucine zipper kinase inhibition preserves neuronal network connectivity. Acta Neuropathologica Communications, 7(1), 93. DOI: 10.1186/s40478-019-0741-3

Wang, G.; Zheng, X.; Duan, H.; Dai, Y.; Niu, Y.; Gao, J.; Zheng, Y. (2019). High-content analysis of particulate matters-induced oxidative stress and organelle dysfunction in vitro. Toxicology in Vitro, 59, 263–274. DOI: 10.1016/j.tiv.2019.04.026

Wang, X.; Liang, D.; Wang, Y.; Ma, Q.; Xing, B.; and Fan, W. (2019). Effects of organic matter on uptake and intracellular trafficking of nanoparticles in Tetrahymena thermophila. Environmental Science: Nano, 6(7), 2116–2128. DOI: 10.1039/C9EN00303G

Weigle, S.; Martin, E.; Voegtle, A.; Wahl, B.; and Schuler, M. (2019). Primary cell-based phenotypic assays to pharmacologically and genetically study fibrotic diseases in Vitro. Journal of Biological Methods, 6(2), e115. DOI: 10.14440/jbm.2019.285

Whalley, HJ.; Porter, AP.; Diamantopoulou, Z.; White, GR.; Castañeda-Saucedo, E.; and Malliri, A. (2015) Cdk1 phosphorylates the Rac activator Tiam1 to activate centrosomal Pak and promote mitotic spindle formation. Nat. Commun. 6(7437). DOI: 10.1038/ncomms8437

Zhang, S.-J.; Li, X.-X.; Yu, Y.; Chiu, A. P.; Lo, L. H.; To, J. C.; Keng, V. W. (2019). Schwann cell-specific PTEN and EGFR dysfunctions affect neuromuscular junction development by impairing Agrin signaling and autophagy. Biochemical and Biophysical Research Communications, 515(1), 50–56. DOI: 10.1016/j.bbrc.2019.05.014

Zhang, Z.; Jiang, D.; Yang, H.; He, Z.; Liu, X.; Qin, W.; Qian, Q. (2019). Modified CAR T cells targeting membrane-proximal epitope of mesothelin enhances the antitumor function against large solid tumor. Cell Death and Disease, 10(7), 1–12. DOI: 10.1038/s41419-019-1711-1

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CANCER RESEARCH

Alexander, C-M.; Xiong, K-N.; Velmurugan, K.; Xiong, J.; Osgood, RS.; and Bauer, AK. (2016). Differential innate immune cell signatures and effects regulated by toll-like receptor four during murine lung tumor promotion. Experimental Lung Research. 42, 154-173.

Amoury, M.; Mladenov, R.; Nachreiner, T.; Pham, AT.; Hristodorov, D.; Di Fiore, S.; Helfrich, W.; Pardo, A.; Fey, G.; and Schwenkert, M. (2016). A novel approach for targeted elimination of CSPG4‐ positive triple‐negative breast cancer cells using a MAP tau‐based fusion protein. International Journal of Cancer. 139(4):916-27.

Anastasov, N.; Höfig, I.; Radulović, V.; Ströbel, S.; Salomon, M.; Lichtenberg, J.; Rothenaigner, I.; Hadian, K.; Kelm, JM.; and Thirion, C. (2015) A 3D-microtissue-based phenotypic screening of radiation resistant tumor cells with synchronized chemotherapeutic treatment. BMC Cancer. 15(466).

Baccelli, I.; Krosl, J.; Boucher, G.; Boivin, I.; Lavallée, V-P.; Hébert, J.; Lemieux, S.; Marinier, A.; and Sauvageau, G. (2017) A novel approach for the identification of efficient combination therapies in primary human acute myeloid leukemia specimens. Blood Cancer Journal 7, e529.; DOI: 10.1038/bcj.2017.10

Bao, B.; Liu, M.; Liu, Y.; Zhang, X.; Zang, Y.; Li, J.; and Lu, W. (2015) NIR absorbing DICPO derivatives applied to wide range of pH and detection of glutathione in tumor. Tetrahedron. 71(41):7865-7868.

Bastian, A.; Thorpe, JE.; Disch, BC.; Bailey-Downs, LC.; Gangjee, A.; Devambatla, RK.; Henthorn, J.; Humphries, KM.; Vadvalkar. SS.; and Ihnat MA. (2015). A Small Molecule with Anticancer and Antimetastatic Activities Induces Rapid Mitochondrial-Associated Necrosis in Breast Cancer. Journal of Pharmacology and Experimental Therapeutics. 353(2):392-404.

Bauer, AK.; Velmurugan, K.; Xiong, KN.; Alexander, CM.; Xiong, J.; and Brooks, R. (2016) Epiregulin is required for lung tumor promotion in a murine two‐stage carcinogenesis model. Molecular Carcinogenesis. DOI: 10.1002/mc.22475.

Bill, R.; Fagiani, E.; Zumsteg, A.; Antoniadis, H.; Johansson, D.; Haefliger, S.; Albrecht, I.; Hilberg, F.; and Christofori, G. (2015) Nintedanib is a highly effective therapeutic for neuroendocrine carcinoma of the pancreas (PNET) in the Rip1Tag2 transgenic mouse model. Clinical Cancer Research. 21(21):4856-67.

Cheng, V.; Esteves, F.; Chakrabarty, A.; Cockle, J.; Short, S.; and Brüning-Richardson, A. (2015) High-content analysis of tumour cell invasion in three-dimensional spheroid assays. Oncoscience. 2(6):596-606.

Cursons, J.; Leuchowius, K-J.; Waltham, M.; Tomaskovic-Crook, E.; Foroutan, M.; Bracken, CP.; Redfern, A.; Crampin, EJ.; Street, I.; and Davis, MJ. (2015) Stimulus-dependent differences in signalling regulate epithelial-mesenchymal plasticity and change the effects of drugs in breast cancer cell lines. Cell Communication and Signaling. 13(26).

Falkenberg, N.; Anastasov, N.; Höfig, I.; Bashkueva, K.; Lindner, K.; Höfler, H.; Rosemann, M.; and Aubele, M. (2015) Additive impact of HER2-/PTK6-RNAi on interactions with HER3 or IGF-1R leads to reduced breast cancer progression in vivo. Molecular Oncology. 9(1):282-294.

Falkenberg, N.; Höfig, I.; Rosemann, M.; Szumielewski, J.; Richter, S.; Schorpp, K.; Hadian, K.; Aubele, M.; Atkinson, MJ.; and Anastasov, N. (2016) Three‐dimensional microtissues essentially contribute to preclinical validations of therapeutic targets in breast cancer. Cancer Medicine. 5(4), 703-10.

Flinders, C.; Lam, L.; Rubbi, L.; Ferrari, R.; Fitz-Gibbon, S.; Chen, P-Y.; Thompson, M.; Christofk, H.; Agus, D.; and Ruderman, D. (2016) Epigenetic changes mediated by polycomb repressive complex 2 and E2a are associated with drug resistance in a mouse model of lymphoma. Genome Medicine. 8(1):54.

Herrera-Abreu, MT.; Palafox, M.; Asghar, U.; Rivas, MA.; Cutts, RJ.; Garcia-Murillas, I.; Pearson, A.; Guzman, M.; Rodriguez, O.; Grueso, J.; Bellet, M.; Cortés, J.; Elliott, R.; Pancholi, S.; Baselga, J.; Dowsett, M.; Martin, LA.; Turner, NC.; and Serra V. (2016) Early Adaptation and Acquired Resistance to CDK4/6 Inhibition in Estrogen Receptor–Positive Breast Cancer. Cancer Research. 76, 2301-2313.

Kumar, CS.; Mahesh, A.; Antoniraj, MG.; Vaidevi, S.; and Ruckmani, K. (2016). Ultrafast synthesis of stabilized gold nanoparticles using aqueous fruit extract of Limonia acidissima L. and conjugated epirubicin: targeted drug delivery for treatment of breast cancer. RSC Advances. 6, 26874-26882.

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Lange, I.; Moschny, J.; Tamanyan, K.; Khutsishvili, M.; Atha, D.; Borris, RP.; and Koomoa, D-L. (2016). Scrophularia orientalis extract induces calcium signaling and apoptosis in neuroblastoma cells. International Journal of Oncology. 48(4), 1608-1616.

Pérez-Guijarro, E.; Karras, P.; Cifdaloz, M.; Martínez-Herranz, R.; Cañón, E.; Graña, O.; Horcajada-Reales, C.; Alonso-Curbelo, D.; Calvo, TG.; Gómez-López, G.; Bellora, N.; Riveiro-Falkenbach, E.; Ortiz-Romero, PL.; Rodríguez-Peralto, JL.; Maestre, L.; Roncador, G.; de Agustín Asensio, JC.; Goding, CR.; Eyras, E.; Megías, D.; Méndez, R.; and Soengas MS. (2016) Lineage-specific roles of the cytoplasmic polyadenylation factor CPEB4 in the regulation of melanoma drivers. Nature Communications, 7(13418). DOI: 10.1038/ncomms13418

Perlíková, P.; Rylová, G.; Nauš, P.; Elbert, T.; Tlou ová, E.; Bourderioux, A.; Slav tínská, LP.; Motyka, K.; Doležal, D.; and Znojek, P. (2016) 7-(2-Thienyl)-7-deazaadenosine (AB61).; a new potent nucleoside cytostatic with a complex mode of action. Molecular Cancer Therapeutics. 15(5), 922-937.

Schang, G.; Robaire, B.; and Hales, BF. (2016). Organophosphate Flame Retardants act as Endocrine-Disrupting Chemicals in MA-10 Mouse Tumor Leydig Cells. Toxicological Sciences. 150(2), 499-509.

Weber, C.; Telerman, SB.; Reimer, AS.; Sequeira, I.; Liakath-Ali, K.; Arwert, EN.; and Watt, FM. (2016) Macrophage infiltration and alternative activation during wound healing promote MEK1-induced skin carcinogenesis. Cancer Research. 76(4), 805-17.

Wengner, AM.; Siemeister, G.; Koppitz, M.; Schulze, V.; Kosemund, D.; Klar, U.; Stoeckigt, D.; Neuhaus, R.; Lienau, P.; and Bader, B. (2016) Novel Mps1 Kinase Inhibitors with Potent Antitumor Activity. Molecular Cancer Therapeutics. 15(4), 583-592.

Zhou, Q.; Abraham, A.; Li, L.; Babalmorad, A.; Bagby, S.; Arcaroli, J.; Hansen, R.; Valeriote, F.; Gustafson, D.; and Schaack, J. (2016) Topoisomerase II mediates TCF-dependent epithelial–mesenchymal transition in colon cancer. Oncogene. DOI: 10.1038/onc.2016.29.

VIRAL DISEASES

Barrows, NJ.; Campos, RK.; Powell, ST.; Prasanth, KR. (2016). A Screen of FDA-Approved Drugs for Inhibitors of Zika Virus Infection. Cell Host and Microbe, DOI: 10.1016/j.chom.2016.07.004

Blyth, GA.; Chan, W-F.; Webster, RG.; and Magor, KE. (2015). Duck IFITM3 mediates restriction of influenza viruses. Journal of Virology, 90(1), 103-116.

Caì, Y.; Postnikova, EN.; Bernbaum, JG.; Yú, S.; Mazur, S.; Deiuliis, NM.; Radoshitzky, SR.; Lackemeyer, MG.; McCluskey, A.; and Robinson, PJ. (2015). Simian hemorrhagic fever virus cell entry is dependent on CD163 and uses a clathrin-mediated endocytosis-like pathway. Journal of Virology, 89(1):844-856.

Chang, SY.; Park, JH.; Kim, YH.; Kang, JS.; and Min, J-Y. (2016). A natural component from Euphorbia humifusa Willd displays novel.; broad-spectrum anti-influenza activity by blocking nuclear export of viral ribonucleoprotein. Biochemical and biophysical research communications, 471(2), 282-9.

Chen, J.; Jiang, L.; Lan, K.; and Chen, X. (2015). Celecoxib Inhibits the Lytic Activation of Kaposi’s Sarcoma-Associated Herpesvirus through Down-Regulation of RTA Expression by Inhibiting the Activation of p38 MAPK. Viruses, 7(5), 2268-2287.

Cortjens, B.; de Boer, OJ.; de Jong, R.; Antonis, AF.; Sabogal Piñeros, YS.; Lutter, R.; van Woensel, J.; and Bem, RA. (2016). Neutrophil Extracellular Traps Cause Airway Obstruction During Respiratory Syncytial Virus Disease. The Journal of Pathology, 238(3), 401-411.

Ekins, S.; Freundlich, JS.; Clark, AM.; Anantpadma, M.; Davey, RA.; and Madrid, P. (2016). Machine learning models identify molecules active against the Ebola virus in vitro. F1000 Research, 4(1091).

Herbert, AS.; Davidson, C.; Kuehne, AI.; Bakken, R.; Braigen, SZ.; Gunn, KE.; Whelan, SP.; Brummelkamp, TR.; Twenhafel, NA.; and Chandran, K. (2015). Niemann-Pick C1 Is Essential for Ebolavirus Replication and Pathogenesis In Vivo. mBio, 6(3), e00565-15.

Holtsberg, FW.; Shulenin, S.; Vu, H.; Howell, KA.; Patel, SJ.; Gunn, B.; Karim, M.; Lai, JR.; Frei, JC.; and Nyakatura, EK. (2015). Pan-ebolavirus and pan-filovirus mouse monoclonal antibodies: protection against Ebola and Sudan viruses. Journal of Virology, 90(1), 266-78.

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Kang, H.; Kim, C.; Kim, D-e.; Song, J-H.; Choi, M.; Choi, K.; Kang, M.; Lee, K.; Kim, HS.; and Shin, JS. (2015). Synergistic antiviral activity of gemcitabine and ribavirin against enteroviruses. Antiviral Research, 124, 1-10.

Kim, H-Y.; Kong, S.; Oh, S.; Yang, J.; Jo, E.; Ko, Y.; Kim, S-H.; Hwang, JY.; Song, R.; and Windisch, MP. (2016). Benzothiazepinecarboxamides: Novel hepatitis C virus inhibitors that interfere with viral entry and the generation of infectious virions. Antiviral Research, 129, 39-46.

McMullan, LK.; Flint, M.; Dyall, J.; Albariño, C.; Olinger, GG.; Foster, S.; Sethna, P.; Hensley, LE.; Nichol, ST.; and Lanier, ER. (2016). The lipid moiety of brincidofovir is required for in vitro antiviral activity against Ebola virus. Antiviral Research, 125, 71-78.

Park, JH.; Park, EB.; Lee, JY.; and Min, J-Y. (2016). Identification of novel membrane-associated prostaglandin E synthase-1 (mPGES-1) inhibitors with anti-influenza activities in vitro. Biochemical and Biophysical Research Communications, 469(4), 848-855.

Phillips, SL.; Soderblom, EJ.; Bradrick, SS.; and Garcia-Blanco, MA. (2016). Identification of Proteins Bound to Dengue Viral RNA In Vivo Reveals New Host Proteins Important for Virus Replication. mBio, 7(1), e01865-15.

Radoshitzky, SR.; Pegoraro, G.; Ch, X.; Dng, L.; Chiang, C-Y.; Jozwick, L.; Clester, JC.; Cooper, CL.; Courier, D.; and Langan, DP. (2016). siRNA Screen Identifies Trafficking Host Factors that Modulate Alphavirus Infection. PLoS Pathogens, 12, e1005466.

BACTERIAL DISEASES

Aljayyoussi, G.; Jenkins, VA.; Sharma, R.; Ardrey, A.; Donnellan, S.; Ward, SA.; and Biagini, GA. (2017). Pharmacokinetic- Pharmacodynamic modelling of intracellular Mycobacterium tuberculosis growth and kill rates is predictive of clinical treatment duration Scientific Reports, 7(502). DOI: 10.1038/s41598-017-00529-6

Ang, ML.; and Pethe, K. (2016). Contribution of high-content imaging technologies to the development of anti- infective drugs. Cytometry A, 89(8), 755-60.

Christophe, T.; Jackson, M.; Jeon, HK.; Fenistein, D.; Contreras-Dominguez, M.; Kim, J.; Genovesio, A.; Carralot, JP.; Ewann, F.; Kim, EH.; Lee, SY.; Kang, S.; Seo, MJ.; Park, EJ.; Skovierová, H.; Pham, H.; Riccardi, G.; Nam, JY.; Marsollier, L.; Kempf, M.; Joly-Guillou, ML.; Oh, T.; Shin, WK.; No, Z.; Nehrbass, U.; Brosch, R.; Cole, ST.; and Brodin, P. (2009). High Content Screening Identifies Decaprenyl-phosphoribose 2’ epimerase as a Target for Intracellular Antimycobacterial Inhibitors. PLoS Pathogens, 5(10), e1000645. DOI: 10.1371/journal.ppat.1000645

Clare, RH.; Cook, DA.; Johnston, KL.; Ford, L.; Ward, SA.; and Taylor, MJ. (2015). Development and validation of a high-throughput anti-Wolbachia whole-cell screen: a route to macrofilaricidal drugs against onchocerciasis and lymphatic filariasis. J Biomol Screen, 20(1), 64-9. DOI: 10.1177/1087057114551518

Delorme, V.; Song, O-R.; Baulard, A.; and Brodin, P. (2015). Testing Chemical and Genetic Modulators in Mycobacterium tuberculosis Infected Cells Using Phenotypic Assays. Methods in Molecular Biology, 1285, 387-411. DOI: 10.1007/978-1-4939-2450-9_24

Lozano-Durán, R.; Bourdais. G.; He, SY.; and Robatzek, S. (2014). The Bacterial Effector HopM1 Suppresses PAMP- triggered Oxidative Burst and Stomatal Immunity. The New Phytologist, 202(1), 259–69. DOI: 10.1111/nph.12651

Mellouk, N.; Weiner, A.; Aulner, N.; Schmitt, C.; Elbaum, M.; Shorte, SL.; Danckaert, A.; and Enninga, J. (2014). Shigella Subverts the Host Recycling Compartment to Rupture its Vacuole. Cell Host and Microbe, 16(4), 517–30. DOI: 10.1016/j.chom.2014.09.005

Quereda, JJ.; Pizarro-Cerdá, J.; Balestrino, D.; Bobard, A.; Danckaert, A.; Aulner, N.; Shorte, S.; Enninga, J.; and Cossart, P. (2016). A Dual Microscopy-based Assay to Assess Listeria monocytogenes Cellular Entry and Vacuolar Escape. Applied and Environmental Microbiology, 82(1), 211–217. DOI: 10.1128/AEM.02302-15

Queval, CJ.; Song, OR.; Deboosère, N.; Delorme, V.; Debrie, AS.; Iantomasi, R.; Veyron-Churlet, R.; Jouny, S.; Redhage, K.; Deloison, G.; Baulard, A.; Chamaillard, M.; Locht, C.; and Brodin P. (2016). STAT3 Represses Nitric Oxide Synthesis in Human Macrophages upon Mycobacterium tuberculosis Infection. Scientific Reports, 6, 29297. DOI: 10.1038/srep29297

7

PARASITIC DISEASES

Alonso-Padilla, J.; Cotillo, I.; Presa, JL.; Cantizani, J.; Peña, I.; Bardera, AI.; Martín, JJ.; and Rodriguez, A. (2015). Automated High-Content Assay for Compounds Selectively Toxic to Trypanosoma cruzi in a Myoblastic Cell Line. PLoS Neglected Tropical Diseases, 9(1), e0003493. DOI: 10.1371/journal.pntd.0003493

De Rycker, M.; Thomas, J.; Riley, J.; Brough, SJ.; Miles, TJ.; and Gray, DW. (2016). Identification of Trypanocidal Activity for Known Clinical Compounds Using a New Trypanosoma cruzi Hit-Discovery Screening Cascade. PLoS Neglected Tropical Diseases, 10(4), e0004584. DOI: 10.1371/journal.pntd.0004584

Duez, J.; Holleran, JP.; Ndour, PA.; Loganathan, S.; Amireault, P.; Français, O.; El Nemer, W.; Le Pioufle, B.; Amado, IF.; and Garcia, S. (2015). Splenic retention of Plasmodium falciparum gametocytes to block the transmission of malaria. Antimicrobial Agents and Chemotherapy, 59(7), 4206-4214. DOI: 10.1128/AAC.05030-14

Fletcher, S.; Lucantoni, L.; Sykes, ML.; Jones, AJ.; Holleran, JP.; Saliba, KJ and Avery, VM. (2016). Biological characterization of chemically diverse compounds targeting the Plasmodium falciparum coenzyme A synthesis pathway. Parasites and Vectors, 9(589). DOI: 10.1186/s13071-016-1860-3

Hart, CJ.; Munro, T.; Andrews, KT.; Ryan, JH.; Riches, AG.; and Skinner-Adams, TS. (2017). A novel in vitro image-based assay identifies new drug leads for giardiasis. International Journal for Parasitology: Drugs and Drug Resistance, 7(1), 83–89. DOI: 10.1016/j.ijpddr.2017.01.005

Khare, S.; Liu, X.; Stinson, M.; Rivera, I.; Groessl, T.; Tuntland, T.; Yeh, V.; Wen, B.; Molteni, V.; and Glynne, R. (2015). Anti- Trypanosomal Treatment with Benznidazole is Superior to Posaconazole Regimens in Mouse Models of Chagas Disease. Antimicrobial agents and chemotherapy, 59(10), 6385-6394. DOI: 10.1128/AAC.00689-15

Khare, S.; Roach, SL.; Barnes, SW.; Hoepfner, D.; Walker, JR.; Chatterjee, AK.; Neitz, RJ.; Arkin, MR.; McNamara, CW.; and Ballard, J. (2015). Utilizing Chemical Genomics to Identify Cytochrome b as a Novel Drug Target for Chagas Disease. PLoS Pathogen, 11(7), e1005058. DOI: 10.1371/journal.ppat.1005058

Lucantoni, L.; Silvestrini, F.; Signore, M.; Siciliano, G.; Eldering, M.; Dechering, KJ.; Avery, VM.; and Alano, P. (2015). A Simple and Predictive Phenotypic High Content Imaging Assay for Plasmodium falciparum Mature Gametocytes to Identify Malaria Transmission Blocking Compounds. Scientific Reports, 5, 16414. DOI: 10.1038/srep16414

Plouffe, DM.; Wree, M.; Du, AY.; Meister, S.; Li, F.; Patra, K.; Lubar, A.; Okitsu, SL.; Flannery, EL.; Kato, N.; Tanaseichuk, O.; Comer, E.; Zhou, B.; Kuhen, K.; Zhou, Y.; Leroy, D.; Schreiber, SL.; Scherer, CA.; Vinetz, J.; and Winzeler, EA. (2016). High-Throughput Assay and Discovery of Small Molecules that Interrupt Malaria Transmission. Cell Host and Microbe, 19(1), 114–126. DOI: 10.1016/j.chom.2015.12.001

Sykes, ML.; and Avery, VM. (2015). Development and Application of a Sensitive.; Phenotypic.; High-throughput Image- based Assay to Identify Compound Activity Against Trypanosoma cruzi Amastigotes. International Journal for Parasitology. Drugs and Drug Resistance, 5(3), 215–28. DOI: 10.1016/j.ijpddr.2015.10.001

Tegazzini, D.; Díaz, R.; Aguilar, F.; Peña, I.; Presa, JL.; Yardley, V.; Martin, JJ.; Coteron, JM.; Croft, SL.; and Cantizani, J. (2016). A replicative in vitro assay for drug discovery against Leishmania donovani. Antimicrobial Agents and Chemotherapy, 60(6), 3524-3532. DOI: 10.1128/AAC.01781-15

NEUROSCIENCE

Ahfeldt, Tim.; Litterman, NK.; and Rubin, LL. (2016). Studying Human Disease Using Human Neurons. Brain Research, S0006-8993(16)30189-5. DOI: 10.1016/j.brainres.2016.03.051

Bonafede, R.; Scambi, I.; Peroni, D.; Potrich, V.; Boschi, F.; Benati, D.; Bonetti, B.; and Mariotti, R. (2016). Exosome derived from murine adipose-derived stromal cells: Neuroprotective effect on in vitro model of amyotrophic lateral sclerosis. Experimental Cell Research, 340(1), 150-158. DOI: 10.1016/j.yexcr.2015.12.009

8

Crippa, V.; D’Agostino, VG.; Cristofani, R.; Rusmini, P.; Cicardi, ME.; Messi, E.; Loffredo, R.; Pancher, M.; Piccolella, M.; and Galbiati, M. (2016). Transcriptional induction of the heat shock protein B8 mediates the clearance of misfolded proteins responsible for motor neuron diseases. Scientific Reports, 6(22827). DOI: 10.1038/srep22827

da Luz, MH.; Peres, IT.; Santos, TG.; Martins, VR.; Icimoto, MY.; and Lee, KS. (2015). Dopamine induces the accumulation of insoluble prion protein and affects autophagic flux. Frontiers in Cellular Neuroscience, 9(12). DOI: 10.3389/fncel.2015.00012

Dakic, V.; de Morae Maciel, R.; Drummond, H.; Nascimento, JM.; Trindade, P.; and Rehen, SK. (2016). Harmine stimulates neurogenesis of human neural cells in vitro. PeerJ Preprints, 4:e1957v1. DOI: 10.7287/peerj.preprints.1957v1

Forster, J.; Köglsberger, S.; Trefois, C.; Boyd, O.; Baumuratov, A.; Buck, L.; Balling, R.; and Antony, P. (2016). Characterization of Differentiated SH-SY5Y as Neuronal Screening Model Reveals Increased Oxidative Vulnerability. Journal of Biomolecular Screening, 21(5), 496-509. DOI: 10.1177/1087057115625190

Gillard, GO.; Collette, B.; Anderson, J.; Chao, J.; Scannevin, RH.; Huss, DJ.; and Fontenot, JD. (2015). DMF.; but not other fumarates.; inhibits NF-‐B activity in vitro in an Nrf2-independent manner. Journal of Neuroimmunology, 283, 74-85. DOI: 10.1016/j.jneuroim.2015.04.006

Khan, FH.; Pandian, V.; Ramraj, S.; Aravindan, S.; Herman, TS.; and Aravindan, N. (2015). Reorganization of metastamiRs in the evolution of metastatic aggressive neuroblastoma cells. BMC Genomics, 16(501). DOI: 10.1186/s12864-015-1642-x

Khan, FH.; Pandian, V.; Ramraj, S.; Natarajan, M.; Aravindan, S.; Herman, TS.; and Aravindan, N. (2015). Acquired genetic alterations in tumor cells dictate the development of high-risk neuroblastoma and clinical outcomes. BMC Cancer, 15(514). DOI: 10.1186/s12885-015-1463-y

Khan, FH.; Pandian, V.; Ramraj, SK.; Aravindan, S.; Natarajan, M.; Azadi, S.; Herman, TS.; and Aravindan, N. (2015). RD3 loss dictates high-risk aggressive neuroblastoma and poor clinical outcomes. Oncotarget, 6(34), 36522-36534. DOI: 10.18632/oncotarget.5204

Kiris, E.; Burnett, JC.; Nuss, JE.; Wanner, LM.; Peyser, BD.; Du, HT.; Gomba, GY.; Kota, KP.; Panchal, RG.; and Gussio, R. (2015). Src Family Kinase Inhibitors Antagonize the Toxicity of Multiple Serotypes of Botulinum Neurotoxin in Human Embryonic Stem Cell-Derived Motor Neurons. Neurotoxicity Research, 27(4), 384-398. DOI: 10.1007/s12640-015-9526-z

Qian, G.; Wu, Y.; Ferrari, D.; Qiao, P.; and Hollande, F. (2016). Semisupervised Clustering by Iterative Partition and Regression with Neuroscience Applications. Computational Intelligence and Neuroscience, 2016(4037380). DOI: 10.1155/2016/4037380

Rudhard, Y.; Sengupta Ghosh, A.; Lippert, B.; Bocker, A.; Pedaran, M.; Kramer, J.; Ngu, H.; Foreman, O.; Liu, Y.; and Lewcock, JW. (2015). Identification of 12/15-lipoxygenase as a regulator of axon degeneration through high- content screening. Journal of Neuroscience, 35(7), 2927-2941. DOI: 10.1523/JNEUROSCI.2936- 14.2015

Guo, SM.; Veneziano, R.; Gordonov, S.; Li, L.; Park, D.; Kulesa, AB.; Blainey, PC.; Cottrell, JR.; Boyden, ES.; and Bathe, M. (2017). Multiplexed confocal and super-resolution fluorescence imaging of cytoskeletal and neuronal synapse proteins. bioRxiv.

Tee, JY.; Sutharsan, R.; Fan, Y.; Mackay-Sim, A. (2016). Schizophrenia patient-derived olfactory neurosphere-derived cells do not respond to extracellular reelin npj Schizophrenia. DOI: 10.1038/npjschz.2016.27

Usenovic, M.; Niroomand, S.; Drolet, RE.; Yao, L.; Gaspar, RC.; Hatcher, NG.; Schachter, J.; Renger, JJ.; and Parmentier-Batteur, S. (2015). Internalized Tau Oligomers Cause Neurodegeneration by Inducing Accumulation of Pathogenic Tau in Human Neurons Derived from Induced Pluripotent Stem Cells. Journal of Neuroscience, 35(42), 14234-14250. DOI: 10.1523/JNEUROSCI.1523-15.2015

Velasquez, JT.; Nazareth, L.; Quinn, R.; Ekberg, J.; and St John, J. (2016). Stimulating the proliferation.; migration and lamellipodia of Schwann cells using low-dose curcumin. Neuroscience, 324, 140-150. DOI: 10.1016/j.neuroscience.2016.02.073

9

TOXICOLOGY

Bernardi, M.; Adami, V.; Albiero, E.; Madeo, D.; Rodeghiero, F.; and Astori, G. (2014). Absence of micronucleus formation in CHO-K1 cells cultivated in platelet lysate enriched medium. Experimental and Toxicologic Pathology, 66(2-3), 111-116.

Billis, P.; Will, Y.; and Nadanaciva, S. (2014). High‐Content Imaging Assays for Identifying Compounds that Generate Superoxide and Impair Mitochondrial Membrane Potential in Adherent Eukaryotic Cells. Current Protocols in Toxicology, 59(25.1), 1-14.

Bramsen, JB.; Laursen, MB.; Nielsen, AF.; Hansen, TB.; Bus, C.; Langkjær, N.; Babu, BR.; Højland, T.; Abramov, M.; and Van Aerschot, A. (2009). A large-scale chemical modification screen identifies design rules to generate siRNAs with high activity.; high stability and low toxicity. Nucleic Acids Research, 37(9), 2867-2881.

Ivask, A.; Voelcker, NH.; Seabrook, SA.; Hor, M.; Kirby, JK.; Fenech, M.; Davis, TP.; and Ke, PC. (2015). DNA Melting and Genotoxicity Induced by Silver Nanoparticles and Graphene. Chemical Research in Toxicology. 28(5), 1023-35.

Kiris, E.; Burnett, JC.; Nuss, JE.; Wanner, LM.; Peyser, BD.; Du, HT.; Gomba, GY.; Kota, KP.; Panchal, RG.; and Gussio, R. (2015). Src Family Kinase Inhibitors Antagonize the Toxicity of Multiple Serotypes of Botulinum Neurotoxin in Human Embryonic Stem Cell-Derived Motor Neurons. Neurotoxicity Research, 27(4), 384-398.

Ku, S-K.; Baek, M-C.; and Bae, J-S. (2015). Anti-inflammatory effects of methylthiouracil in vitro and in vivo. Toxicology and Applied Pharmacology. 288(3), 374-86.

Schang, G.; Robaire, B.; and Hales, BF. (2016). Organophosphate Flame Retardants act as Endocrine-Disrupting Chemicals in MA-10 Mouse Tumor Leydig Cells. Toxicological Sciences, 150(2), 499-509.

Van de Water, F.; Havinga, J.; Ravesloot, W.; Horbach, G.; and Schoonen, W. (2011). High content screening analysis of phospholipidosis: validation of a 96-well assay with CHO-K1 and HepG2 cells for the prediction of in vivo based phospholipidosis. Toxicology in Vitro, 25(8), 1870-1882.

Wang, M.; Liu, C-X.; Dong, R-R.; He, S.; Liu, T-T.; Zhao, T-C.; Wang, Z-L.; Shen, X-Y.; Zhang, B-L.; and Gao, X-M. (2015). Safety Evaluation of Chinese Medicine Injections with a Cell ImagingBased Multiparametric Assay Revealed a Critical Involvement of Mitochondrial Function in Hepatotoxicity. Evidence-Based Complementary and Alternative Medicine, 379586.

Willy, JA.; Schulte, NE.; Kreklau, EL.; Walgren, JL.; Renninger, ML.; and Baker, TK. (2016). In Vitro L6 Irritation Assay Predicts Clinical Injection Site Reactions for Small Molecules. Toxicological Sciences, 151(2), 302-11.

Ye, Y.; Weiwei, J.; Na, L.; Mei, M.; Kaifeng, R.; and Zijian, W. (2014). Application of the SOS/umu test and high‐content in vitro micronucleus test to determine genotoxicity and cytotoxicity of nine benzothiazoles. Journal of Applied Toxicology. 34, 1400-1408.

STEM CELLS

Abdallah, BM.; Al-Shammary, A.; Skagen, P.; Dawud, RA.; Adjaye, J.; Aldahmash, A.; and Kassem, M. (2015). CD34 defines an osteoprogenitor cell population in mouse bone marrow stromal cells. Stem Cell Research, 15(2), 449-458. DOI: 10.1016/j.scr.2015.09.005

Ahmed, SU.; Carruthers, R.; Gilmour, L.; Yildirim, S.; Watts, C.; and Chalmers, AJ. (2015). Selective inhibition of parallel DNA damage response pathways optimizes radiosensitization of glioblastoma stem-like cells. Cancer Research, 75(20), 4416-4428. DOI: 10.1158/0008-5472

Andersen, RK.; Zaher, W.; Larsen, KH.; Ditzel, N.; Drews, K.; Wruck, W.; Adjaye, J.; Abdallah, BM.; and Kassem, M. (2015). Association between in vivo bone formation and ex vivo migratory capacity of human bone marrow stromal cells. Stem Cell Research and Therapy, 6(196). DOI: 10.1186/s13287-015-0188-9

Antonczak, A.; Mullee, L.; Wang, Y.; Comartin, D.; Inoue, T.; Pelletier, L.; and Morrison, C. (2016). Opposing effects of pericentrin and microcephalin on the pericentriolar material regulate CHK1 activation in the DNA damage response. Oncogene, 35, 2203-2010. DOI: 10.1038/onc.2015.257

10

Aravindan, S.; Ramraj, SK.; Somasundaram, ST.; and Aravindan, N. (2015). Novel adjuvants from seaweed impede autophagy signaling in therapy-resistant residual pancreatic cancer. Journal of Biomedical Science, 22(28). DOI: 10.1186/s12929-015-0132-4

Bader, AM.; Klose, K.; Bieback, K.; Korinth, D.; Schneider, M.; Seifert, M.; Choi, Y-H.; Kurtz, A.; Falk, V.; and Stamm C. (2015). Hypoxic Preconditioning Increases Survival and Pro-Angiogenic Capacity of Human Cord Blood Mesenchymal Stromal Cells In Vitro. PloS One, 10(9), e0138477. DOI: 10.1371/journal.pone.0138477

Brodarac, A.; Šarić, T.; Oberwallner, B.; Mahmoodzadeh, S.; Neef, K.; Albrecht, J.; Burkert, K.; Oliverio, M.; Nguemo, F.; and Choi, Y-H. (2015). Susceptibility of murine induced pluripotent stem cell-derived cardiomyocytes to hypoxia and nutrient deprivation. Stem Cell Research and Therapy, 6(83). DOI: 10.1186/s13287-015-0057-6

Calder, EL.; Tchieu, J.; Steinbeck, JA.; Tu, E.; Keros, S.; Ying, S-W.; Jaiswal, MK.; Cornacchia, D.; Goldstein, PA.; and Tabar, V. (2015). Retinoic Acid-Mediated Regulation of GLI3 Enables EfficientMotoneuron Derivation from Human ESCs in the Absence of Extrinsic SHH Activation. The Journal of Neuroscience, 35(33), 11462-11481. DOI: 10.1523/JNEUROSCI.3046-14.2015

Celiz, AD.; Smith, JG.; Patel, AK.; Hook, AL.; Rajamohan, D.; George, VT.; Flatt, L.; Patel, MJ.; Epa, VC.; and Singh, T. (2015). Discovery of a novel polymer for human pluripotent stem cellexpansion and multilineage differentiation. Advanced Materials, 27(27), 4006-4012. DOI: 10.1002/adma.201501351

Chen, L.; Shi, K.; Frary, CE.; Ditzel, N.; Hu, H.; Qiu, W.; and Kassem, M. (2015). Inhibiting actindepolymerization enhances osteoblast differentiation and bone formation in humanstromal stem cells. Stem Cell Research, 15(2), 281-289. DOI: 10.1016/j.scr.2015.06.009

Choudhary, S.; Pardo, A.; Rosinke, R.; Batra, JK.; Barth, S.; and Verma, RS. (2016). Targeting c-kit receptor in neuroblastomas and colorectal cancers using stem cell factor (SCF)-based recombinant bacterial toxins. Applied microbiology and biotechnology, 100(1), 263-277. DOI: 10.1007/s00253-015-6978-2

Costa, V.; Aigner, S.; Vukcevic, M.; Sauter, E.; Behr, K.; Ebeling, M.; Dunkley, T.; Friedlein, A.; Zoffmann, S.; and Meyer, CA. (2016). mTORC1 Inhibition Corrects Neurodevelopmental and Synaptic Alterations in a Human Stem Cell Model of Tuberous Sclerosis. Cell Reports, 15(1), 86-95. DOI: 10.1016/j.celrep.2016.02.090

Földes, G.; Matsa, E.; Kriston-Vizi, J.; Leja, T.; Amisten, S.; Kolker, L.; Kodagoda, T.; Dolatshad, NF.; Mioulane, M.; Vauchez, K.; Arányi, T.; Ketteler, R.; Schneider, MD.; Denning, C.; and Harding, SE. (2014). Aberrant α-Adrenergic Hypertrophic Response in Cardiomyocytes from Human Induced Pluripotent Cells Stem Cell Reports, 3(5), 905-914. DOI: 10.1016/j.stemcr.2014.09.002

Ghaemi, SR.; Delalat, B.; Cetó, X.; Harding, FJ.; Tuke, J.; and Voelcker, NH. (2015). Synergistic influence of collagen I and BMP 2 drives osteogenic differentiation of mesenchymal stem cells: A cell microarray analysis. Acta Biomaterialia, 34, 41-52. DOI: 10.1016/j.actbio.2015.07.027

Hildebrand, L.; Rossbach, B.; Kühnen, P.; Gossen, M.; Kurtz, A.; Reinke, P.; Seemann, P.; and Stachelscheid, H. (2016). Generation of integration free induced pluripotent stem cells from fibrodysplasia ossificans progressiva (FOP) patients from urine samples. Stem Cell Research, 16(1), 54-58. DOI: 10.1016/j.scr.2015.11.017

Hildebrand, L.; Seemann, P.; Kurtz, A.; Hecht, J.; Contzen, J.; Gossen, M.; and Stachelscheid, H. (2015). Selective cell targeting and lineage tracing of human induced pluripotent stem cells using recombinant avian retroviruses. Cellular and Molecular Life Sciences, 72(23), 4671-4680. DOI: 10.1007/s00018-015-1957-4

Kaufmann, M.; Schuffenhauer, A.; Fruh, I.; Klein, J.; Thiemeyer, A.; Rigo, P.; Gomez-Mancilla, B.; Heidinger-Millot, V.; Bouwmeester, T.; and Schopfer, U. (2015) High-Throughput Screening Using iPSC-Derived Neuronal Progenitors to Identify Compounds Counteracting Epigenetic Gene Silencing in Fragile X Syndrome. Journal of Biomolecular Screening, 20(9), 1101-1111. DOI: 10.1177/1087057115588287

Kiris, E.; Burnett, JC.; Nuss, JE.; Wanner, LM.; Peyser, BD.; Du, HT.; Gomba, GY.; Kota, KP.; Panchal, RG.; and Gussio, R. (2015) Src Family Kinase Inhibitors Antagonize the Toxicity of Multiple Serotypes of Botulinum Neurotoxin in Human Embryonic Stem Cell-Derived Motor Neurons. Neurotoxicity Research, 27(4):384-398. DOI: 10.1007/s12640-015-9526-z

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Leha, A.; Moens, N.; Meleckyte, R.; Culley, OJ.; Gervasio, MK.; Kerz, M.; Reimer, A.; Cain, SA.; Streeter, I.; Folarin, A.; Stegle, O.; Kielty, CM.; HipSci Consortium.; Durbin, R.; Watt, FM.; and Danovi D. (2016) A high-content platform to characterise human induced pluripotent stem cell lines. Methods, 96, p85-96. DOI: 10.1016/j.ymeth.2015.11.012

Najm, FJ.; Madhavan, M.; Zaremba, A.; Shick, E.; Karl, RT.; Factor, DC.; Miller, TE.; Nevin, ZS.; Kantor, C.; and Sargent, A. (2015). Drug-based modulation of endogenous stem cells promotes functional remyelination in vivo. Nature, 522(7555), 216-220. DOI: 10.1038/nature14335

Pandian, V.; Ramraj, S.; Khan, FH.; Azim, T.; and Aravindan, N. (2015). Metastatic neuroblastoma cancer stem cells exhibit flexible plasticity and adaptive stemness signaling. Stem Cell Research and Therapy, 6(2). DOI: 10.1186/s13287-015-0002-8

Rossbach, B.; Hildebrand, L.; El-Ahmad, L.; Stachelscheid, H.; Reinke, P.; and Kurtz, A. (2016) Generation of a human induced pluripotent stem cell line from urinary cells of a healthy donor using an integration free vector. Stem Cell Research, 16(1), 133-136. DOI: 10.1016/j.scr.2015.12.021

3D CELL MODELS AND CELL CULTURES

Anastasov, N.; Höfig, I.; Radulović, V.; Ströbel, S.; Salomon, M.; Lichtenberg, J.; Rothenaigner, I.; Hadian, K.; Kelm, JM.; and Thirion, C. (2015). A 3D-microtissue-based phenotypic screening of radiation resistant tumor cells with synchronized chemotherapeutic treatment. BMC Cancer, 15(466). DOI: 10.1186/s12885-015-1481-9

Falkenberg, N.; Höfig, I.; Rosemann, M.; Szumielewski, J.; Richter, S.; Schorpp, K.; Hadian, K.; Aubele, M.; Atkinson, MJ.; and Anastasov. N. (2016). Three‐dimensional microtissues essentially contribute to preclinical validations of therapeutic targets in breast cancer. Cancer Medicine, 5(4), 703-10. DOI: 10.1002/cam4.630

KristonćVizi, J.; and Flotow, H. (2016). Getting the whole picture: High content screening using three‐dimensional cellular model systems and whole animal assays. Cytometry Part A. DOI: 10.1002/cyto.a.22907

Letzsch, S.; Boettcher, K.; Kelm, JM.; and Messner, S. (2015) Quantifying Efflux Activity in 3D Liver Spheroids : New Confocal Imaging Instruments Allow Screening in Complex Human Liver Microtissues. Genetic Engineering and Biotechnology News, 35(7), 14-15. DOI: 10.1089/gen.35.07.08

Li, L.; Zhou, Q.; Voss, TC.; Quick, KL.; and LaBarbera, DV. (2016). High-throughput imaging: Focusing in on drug discovery in 3D. Methods, 96, 97–102. DOI: 10.1016/j.ymeth.2015.11.013

Reid, BG.; Jerjian, T.; Patel, P.; Zhou, Q.; Yoo, BH.; Kabos, P.; Sartorius, CA. and LaBarbera, DV. (2014) Live Multicellular Tumor Spheroid Models For High-Content Imaging and Screening In Cancer Drug Discovery. Current Chemical Genomics and Translational Medicine, 8, Suppl-1 27-35. DOI: 10.2174/2213988501408010027

Song, Y.; Kim, SH.; Kim, KM.; Choi, EK.; Kim, J.; and Seo, HR. (2016). Activated hepatic stellate cells play pivotal roles in hepatocellular carcinoma cell chemoresistance and migration in multicellular tumor spheroids. Sci Rep, 17(6), 36750. DOI: 10.1038/srep36750

Trask, OJ.; LaBarbera, DV.; and Quick, KL. (Fall 2016). 3D cellular imaging advances and considerations for high-content screening. Drug Discovery World (Fall 2016).