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Figure S1. A. *. B. Relative pS2 levels. *. **. **. **. C. D. **. *. *. Relative c- Myc levels. Relative PR levels. Relative GREB1 levels. *. *. **. **. **. **. **. **. **. **. **. Figure S2. MCF-7 cells. B. A. **. C. MCF-7:PF cells. Relative pS2 levels. *. - PowerPoint PPT Presentation
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con E2 4-OHT ICI E2+ICI 4-OHT+ICI
0
0.2
0.4
0.6
0.8
1
1.2
1.4
1.6
1.8
2
Rel
ativ
e c-
Myc
leve
ls
*
*
** ** **
Figure S1
A B
CD
Rel
ativ
e p
S2
leve
ls
con E2 4-OHT ICI E2+ICI 4-OHT+ICI
0
0.5
1
1.5
2
2.5 *
*
** ** **
Rel
ativ
e P
R le
vels
con E2 4-OHT ICI E2+ICI 4-OHT+ICI
0
0.5
1
1.5
2
2.5 **
*
**** **
con E2 4-OHT ICI E2+ICI 4-OHT+ICI
0
0.5
1
1.5
2
2.5
3
3.5
4
Rel
ativ
e G
RE
B1
leve
ls
*
** ** **
con E2 4-OHT E2+4-OHT
0
5
10
15
20
25
30
MCF-7:5C cells
Rel
ativ
e p
S2
leve
ls
**
con E2 4-OHT E2+4-OHT
0
10
20
30
40
50
60
70
80
MCF-7 cells
**
Rel
ativ
e p
S2
leve
lsFigure S2
A B C MCF-7:PF cells
Rel
ativ
e p
S2
leve
ls
con E2 4-OHT E2+4-OHT
0
0.5
1
1.5
2
2.5
*
*
con E2 4-OHT
β-actin
p-c-Jun Ser63
p-c-Fos Ser 32
Figure S3
- + - +
- - + +PP2
4-OHT
p-FAK Tyr397
p-STAT3 Tyr705
β-actin
con E2 PP2 E2+PP20
50
100
150
200
250
300
350
DN
A c
onte
nt
(% o
f co
ntr
ol) **
**
A B
C
Figure S4
CD-73
MC
F-7:
5C
MC
F-7:
PF
Plasma membrane
LDH-AM
CF-
7:5C
M
CF-
7:PF
Cytoplasm
MC
F-7:
5C
MC
F-7:
PF
Nucleus
ERα
Markers
Lamin-A/C
D E
con 4-OHT PP2 4-OHT+
PP2
0
50
100
150
200
250
300
DN
A c
onte
nt (
% o
f co
ntr
ol)
**
DN
A c
onte
nt
(% o
f co
ntr
ol)
con E2
FAK inhib
itor
FAK inhib
itor +
E20
50
100
150
200
250
300
350
400
450 **
**
**
Figure S5
IB:IGF1-Rβ
IP:IGF1-Rβ
IB: 4G10
con PP2
