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VIEWS amp REVIEWS
Larry Steinman MDAmit Bar-Or MD PhDJacinta M Behne MADaniel Benitez-Ribas
PhDPeter S Chin MDMichael Clare-Salzler
MDDonald Healey PhDJames I Kim PhDDavid M Kranz PhDAndreas Lutterotti MD
PhDRoland Martin MD
PhDSven Schippling MD
PhDPablo Villoslada MD
PhDCheng-Hong Wei PhDHoward L Weiner MDScott S Zamvil MD
PhDMichael R Yeaman PhDTerry J Smith MDOn behalf of the Guthy-
Jackson CharitableFoundationInternational ClinicalConsortium
Correspondence toDr YeamanMRYeamanuclaedu
Supplemental dataat Neurologyorgnn
Restoring immune tolerance inneuromyelitis opticaPart I
ABSTRACT
Neuromyelitis optica (NMO) and spectrum disorder (NMOSD) represent a vexing process and itsclinical variants appear to have at their pathogenic core the loss of immune tolerance to theaquaporin-4 water channel protein This process results in a characteristic pattern of astrocytedysfunction loss and demyelination that predominantly affects the spinal cord and optic nervesAlthough several empirical therapies are currently used in the treatment of NMOSD none hasbeen proven effective in prospective adequately powered randomized trials Furthermore mostof the current therapies subject patients to long-term immunologic suppression that can causeserious infections and development of cancers The following is the first of a 2-part descriptionof several key immune mechanisms in NMOSD that might be amenable to therapeutic restorationof immune tolerance It is intended to provide a roadmap for how potential immune tolerancerestorative techniques might be applied to patients with NMOSD This initial installment providesa background rationale underlying attempts at immune tolerization It provides specific examplesof innovative approaches that have emerged recently as a consequence of technical advances Inseveral autoimmune diseases these strategies have been reduced to practice Therefore in the-ory the identification of aquaporin-4 as the dominant autoantigen makes NMOSD an ideal can-didate for the development of tolerizing therapies or cures for this increasingly recognizeddisease Neurol Neuroimmunol Neuroinflamm 20163e276 doi 101212NXI0000000000000276
GLOSSARYAPC 5 antigen-presenting cell AQP4 5 aquaporin 4 CAR 5 chimeric antigen receptor DC 5 dendritic cell EAE 5 exper-imental autoimmune encephalomyelitis HLA 5 human leukocyte antigen IgG 5 immunoglobulin G IL 5 interleukin MHC 5major histocompatibility complex NMO 5 neuromyelitis optica PBMC 5 peripheral blood mononuclear cell scFv 5 singlechain variable fragment SD 5 spectrum disorder T1D 5 type 1 diabetes TcR 5 T cell receptor Treg 5 regulatory T cell
RATIONALE FOR TOLERIZATION IN NMO Neuromyelitis optica (NMO) or NMO spectrum disorder(NMOSD) represents a potentially devastating neuroinflammatory disease in which the spinal cord and opticnerve(s) preferentially undergo astrocyte damage and demyelination1 Recent investigations into its pathogen-esis have demonstrated NMOSD to be distinct from multiple sclerosis (MS) (figure 1)2 Identification ofpathogenic anti-aquaporin 4 (anti-AQP4) antibody (NMOndashimmunoglobulin G [IgG])3 in context of otherclinical characteristics now secures a diagnosis in seropositive disease
From the Department of Neurology (LS) Stanford University School of Medicine Palo Alto CA Neuroimmunology Unit and ExperimentalTherapeutics Program (AB-O) Montreal Neurological Institute and Hospital McGill University Montreal Canada The Guthy-JacksonCharitable Foundation (JMB) San Diego CA Department of Gastroenterology (DB-R PV) Hospital Cliacutenic CIBERehd and Center ofNeuroimmunology amp Inflammatory Bowel Disease Institut drsquoInvestigacions Biomegravediques August Pi Sunyer (IDIBAPS) Barcelona SpainGenentech Inc (PSC) South San Francisco CA Department of Pathology (MC-S) University of Florida School of Medicine GainesvilleOpexa Therapeutics (DH) The Woodlands TX Department of Surgery (JIK) Center for Transplantation Sciences Massachusetts GeneralHospital Harvard Medical School Boston MA Department of Biochemistry (DMK) University of Illinois Urbana Neuroimmunology and MSResearch (AL RM SS) Department of Neurology University Hospital Zurich University Zurich Switzerland Forest Landing Court(HLW) Rockville MD Ann Romney Center for Neurologic Diseases (SSZ) Department of Neurology Brigham and Womenrsquos HospitalHarvard Medical School Boston MA Department of Neurology and Program in Immunology (HLW) University of California San FranciscoSchool of Medicine Department of Medicine (SSZ) Divisions of Molecular Medicine amp Infectious Diseases David Geffen School of Medicine atUCLA Los Angeles Harbor-UCLA Medical Center amp LABioMed at Harbor-UCLA Medical Center (MRY) Torrance CA Department ofOphthalmology and Visual Sciences Kellogg Eye Center and Division of Metabolism and Endocrine Diseases Department of Internal MedicineUniversity of Michigan Medical School Ann Arbor (TJS)
The Guthy-Jackson Charitable Foundation International Clinical Consortium coinvestigators are listed at Neurologyorgnn
Funding information and disclosures are provided at the end of the article Go to Neurologyorgnn for full disclosure forms The Article ProcessingCharge was paid by Guthy-Jackson Charitable Foundation
This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivatives License 40 (CCBY-NC-ND) which permits downloading and sharing the work provided it is properly cited The work cannot be changed in any way or usedcommercially
Neurologyorgnn copy 2016 American Academy of Neurology 1
ordf 2016 American Academy of Neurology Unauthorized reproduction of this article is prohibited
Despite substantial advances in understandingNMOSD no existing regimen has been unambigu-ously proven to modify disease course induce perma-nent remission or restore lost neurologic function inadequately powered prospective clinical trials Con-tinuing reliance on nonspecific agents including cor-ticosteroids azathioprine mycophenolate and B celldepletion45 exposes patients to potentially seriousadverse effects Absence of animal models with com-plete fidelity to human NMOSD has been a histori-cal impediment to novel therapies
Improved diagnosis has facilitated greater focuson developing specific therapies Antigen-specific ap-proaches could restore immune tolerance to AQP4and minimize epitope spreading In NMOSD dys-functions in checkpoints governing immune cell devel-opment contribute to autoreactive immunity (figure 2)Considerable progress has been made in tolerizingstrategies used to treat allergies6 transplant and graftrejection7 and drug hypersensitivity8 In theoryantigen-targeted approaches spare normal immunefunctions To date similar strategies have yielded onlylimited success in other autoimmune diseases such astype 1 diabetes (T1D)9 relapsing-remitting MS10 andinflammatory bowel disease11 Results thus far supportthe general concept that inhibition of antigen-specificT cell andor B cell responses is achievable and may bedurable However in some experimental models tol-erization strategies have worsened disease12 perhapsbecause of uncertain disease etiology Hence in thespecific context of NMOSD tolerization might havedistinct advantages
The Guthy-Jackson Charitable Foundation con-vened a meeting of experts in development ofantigen-specific therapy Key goals of this meetingwere to ascertain the state-of-the-art in immune toler-ization as it might be applied to NMOSD and to cat-alyze innovative thinking The following discussionconsiders specific opportunities and challenges toapply tolerization therapy to solve NMOSD
POTENTIAL STRATEGIES FOR TOLERIZATIONIN NMO Inverse DNA vaccination Dolgin13 andSteinman14 have built on principles of classic desen-sitization originating with Jenner While traditionalvaccines induce immune responses to specific anti-gens inverse vaccines promote immune toleranceDiseases in which a known antigen underlies thepathophysiology would in theory represent the mostpromising candidates for tolerizing therapies Thusseropositive NMOSD should be ideal for antigen-specific therapy as autoimmune responses to AQP4are believed to be fundamental to the disease15ndash17 Inclinical trials pathogenic vs tolerogenic immuneresponses of T and B cells would necessarily bemonitored Other diseases with similarly implicatedautoantigens include myasthenia gravis (acetylcholinereceptor) Graves disease (thyrotropin receptor andthyroglobulin) and T1D (multiple insulin granuleantigens)18
Inverse vaccination with DNA encoding proinsu-lin to reinforce tolerogenic immune checkpoints hasbeen evaluated in patients in an early-phase clinicaltrial in T1D1819 The results suggest that immune
Figure 1 Integrative model of NMOSD pathogenesis
Distinct stages of disease are illustrated as relative temporal and spatial comparators in NMOSD progression Note that over the course of disease pro-gression and demyelination additional autoantigens may be revealed andor recognized potentially resulting in antigen spreading and expansioncompound-ing of autoreactivity A 5 astrocyte (AQP4-expressing cells that support neuronal viability and function) AQP4 5 aquaporin 4 B 5 B lymphocyte BBB 5
blood-brain barrier E5 eosinophil M5monocytemacrophage N5 neuron NK5 natural killer cell NMOSD5 neuromyelitis opticaspectrum disorder P5
polymorphonuclear leukocyte (neutrophil) PER 5 periphery T 5 T lymphocyte
2 Neurology Neuroimmunology amp Neuroinflammation
ordf 2016 American Academy of Neurology Unauthorized reproduction of this article is prohibited
tolerance to proinsulin can be induced (p 0026)The study revealed an inverse relationship betweenantigen-restricted CD81 T cells and serum proinsulinlevels18 A similar strategy has enabled proof-of-principleof altered responses to myelin basic protein in patientswith MS2021 Although such approaches are attractiverelevant antigenic targets likely differ among patientsand may change over time For example myelin oligo-dendrocyte glycoprotein may drive unique pathogenesisin NMOSD and require specific tolerization strategiesMoreover epitopes may differ in peptides as comparedto parent proteins and lipid or carbohydrate modifica-tion may influence presentation
Inverse DNA vaccination targeting tolerizationagainst AQP4 immunoreactivity has also been exam-ined in experimental models of NMOSD A plasmidencoding AQP4 attenuated T and B cell responses spe-cifically to this antigen attesting to antigen specificityof this strategy (unpublished observations) In NMOSD clinical trials may be designed based on one ormore diagnostic biomarker(s) of NMO-IgG seroposi-tivity In this approach a diagnostic biomarker(s) isused as a surrogate of therapeutic efficacy and durability
and serves as an outcome measure This scenario ap-proaches an idealized situation in which biomarkersare both the principal diagnostic gating targets andthe major pathogenic factor(s)
Antiautoreactive T cell vaccines Roles played by autor-eactive T cells in mediating autoimmunity have beenestablished in several animal models and comple-mented observations made in human disease22 Inturn therapeutic modulation of autoreactive T cellfrequency andor function in autoimmune diseasesappears achievable23 However regulation of allimmune responses results from the ldquochecks and bal-ancesrdquo imposed by both effector and regulatory T cellnetworks (figure 3) Thus nonspecific depletion ofeffector T cells could diminish immune surveillanceleading to infections or cancer24 Similarly indiscrim-inate activation or depletion of regulatory T cells(Tregs) may also result in immune dysfunction Inparticular Tregs are capable of limiting the frequencyandor activity of individual autoreactive T cell clonesthrough recognition of their unique T cell receptor(TcR) idiotypes25 When T cells aberrantly recognize
Figure 2 Comparative milestones in T and B lymphocyte maturation
(A) T cell development (B) B cell development Each lineage is represented with distinguishing markers for corresponding stages and where cell commitmentoccurs Arrows indicate potential points along the development pathway where immune tolerizing interventions might be targeted in neuromyelitis opticaspectrum disorder CAR5 chimeric antigen receptor CD5 class designation marker CLP5 common lymphoid precursor DN5 double-negative thymocyteDP5 double-positive CD41CD81 thymocyte Ig5 immunoglobulin ImmB5 immature B cell ISP5 immature single-positive CD81 thymocyte LPreB5 largePreB MatB 5mature B cell PreB 5 pre-B cell receptor B cell ProB 5 progenitor B cell SP 5 single positive CD41 or CD81 thymocyte SPreB 5 small PreBTcR 5 T cell receptor Treg 5 regulatory T cell
Neurology Neuroimmunology amp Neuroinflammation 3
ordf 2016 American Academy of Neurology Unauthorized reproduction of this article is prohibited
self-antigens in an absence of adequate regulatorycounterbalance autoimmune disease might result26
In NMOSD the pathogenic antibody response isdominated by class-switched IgG indicating anunderlying AQP4-reactive T cell activity2728 Inaddition Th17 polarization of naive T cells leads tothe secretion of proinflammatory cytokines in relationto target lesions including interleukin (IL)-17A andgranulocyte macrophagendashcolony stimulating factor29
Such a proinflammatory cytokine profile maypromote tissue destruction through recruitment ofgranulocytes or other cells capable of antibody-dependent cell cytotoxicity
The observation that anti-idiotypic Tregs can arisespontaneously and be associated with the resolution ofautoimmunity led to the concept of T cell vaccina-tion3031 This strategy invokes multiple immune func-tions that may mitigate autoimmune disease Forexample induction of idiotype-restricted CD41CD251FoxP31 Tregs IL-10ndashsecreting CD41 Tr1cells and CD81 cytotoxic T cells can result each capableof selectively targeting autoreactive T cells that expressthe idiotype represented by the immunizing T cells32
T cellndashbased vaccines typically use attenuatedautologous autoreactive effector T cells as immuno-gens32 in an amplification strategy designed to targetand counteract autoreactive T cells Theoreticallythis process should selectively deplete andor func-tionally attenuate pathogenic T cells carrying the cor-responding idiotype Restoring an adequate Treg
influence theoretically should mitigate tissue dysfunc-tion Clinical trials have suggested a therapeutic ben-efit from T cell vaccination with minimal sideeffects33 In this regard proof-of-principle has beendemonstrated in several animal models31 In additiontherapeutic trials applying this strategy to MS rheu-matoid arthritis and systemic lupus erythematosusndashassociated nephritis have been undertaken In some ofthese conditions a T cellndashbased vaccine approach hasdemonstrated considerable promise34ndash36
The manufacture of autologous T cell vaccines isdependent first on identifying autoantigen-specificT cells within the peripheral blood of that patientThis goal is achieved by performing an epitope profil-ing assay using overlapping peptides comprising theamino acid sequence of the target antigen The assayreadout is based on the detection of proinflammatorycytokines induced by immunodominant peptideswithin the target antigen This strategy thereby iden-tifies peptides and the associated T cell responsesmost relevant to the autoimmune disease in anaffected individual Next bulk T cell lines areexpanded in vitro from an autologous peripheralblood sample against the selected peptides to providesufficient numbers of autoreactive T cells to meetdosing requirements These particular T cells are ali-quoted and cryopreserved On demand a dose-equivalent is thawed formulated and attenuatedby irradiation before subcutaneous administrationMethods currently in use allow a single-cycle
Figure 3 Hypothesized cellular and molecular crosstalk influencing NMOSD
(A) Theoretical proinflammatory interactions that arise from loss of immune tolerance which may promote the onset or pro-gression of neuromyelitis opticaspectrum disorder (NMOSD) (B) Theoretical anti-inflammatory interactions that arise andcould be amenable to therapeutic intervention in restoring immune tolerance in NMOSD (Cellular) APC5 antigen-present-ing cell Bc5B cell Tc5 T cell (cell surface determinants) CD5 class designation MHC5major histocompatibility complex(soluble) BAFF 5 B cell activating factor IL 5 interleukin IL-18BP 5 IL-18 binding protein sIL-1R 5 soluble IL-1 receptorsTNFR 5 soluble tumor necrosis factor a receptor
4 Neurology Neuroimmunology amp Neuroinflammation
ordf 2016 American Academy of Neurology Unauthorized reproduction of this article is prohibited
manufacturing process from one blood collection tobe used to generate sufficient drug for at least 1 yearof treatment
Considering the aggregate evidence a compellingcase can be made for the critical role of T cells inthe pathogenesis of NMOSD29 It follows thatreestablishment of immune tolerance to AQP4through vaccination with autologous attenuatedand idiotype-restricted T cells represents a promisingnew approach to immunotherapy in this disease
Dendritic cell vaccines Dendritic cells (DCs) presentantigens to immune cells including those with regula-tory and autoreactive function through major histo-compatibility complex (MHC) class I and IIrestriction Normally DCs present self-antigens butdo not drive autoreactive immune responses Thusautoreactive T cells are inactivated or anergizedbecause of low MHC expression inadequatecostimulation (eg inducing anergy) or tolerogeniccostimulation (eg PD-L1 [programed death ligand1] IL-10 etc) In addition DCs have important rolesin maintaining tolerance by promoting regulatorycells such as CD41 and CD81 Tregs and IL-10ndashproducing regulatory B cells37 Immature human DCsare readily generated in the laboratory from bloodmonocytes and can be engineered to maintaintolerogenic phenotypes Indeed DC-based therapieshave now entered clinical trials focusing on NMOSD(ClinicalTrialsgov identifier NCT02283671)
As a strategy for inducing immune tolerance inT1D DC administration has proven relatively safe38ndash42 Here granulocyte macrophagendashcolony stimulatingfactorIL-4 was used to generate immature CD11c1
DCs from monocytes in vitro Transition to a proin-flammatory DC phenotype is concerning when DCsare administered to human patients To mitigate thisconcern immature DCs can be treated in vitro withimmunosuppressive agents such as corticosteroids vita-min D3 andor IL-1043 before their use as tolerizingvaccines The resulting DCs exhibit durable tolerogenicphenotypes that prolong organ allograft survival inmice Notably this conditioning enhances the tolero-genic properties of DCs44 and induces high-level IL-10production which inhibit activated Th1 and Th17cells induce Tregs and suppress autoreactive B cellfunctions A related strategy involves loading of imma-ture DCs with antisense oligonucleotides targeting keycostimulatory molecules such as CD40 CD80 orCD86 Alternatively viral vectors encoding immuno-regulatory factors may potentiate tolerogenic DCs Ofnote the maintenance of DC immaturity also allowsthese cells to migrate into lymph nodes following anti-gen exposure enhancing their tolerogenic potential
Loading immature DCs with either native or syn-thetic peptides or whole antigens may also promote
antigen-specific tolerance through induction of regula-tory lymphocytes This strategy addresses multiple auto-antigens andor epitopes within a target antigen(s)Thus the goal is conditional presentation of self-antigenic epitopes by DCs to yield Treg inductionUsing the entire antigen affords presentation of a broad-er repertoire of peptides selected by relevant MHC al-leles and potentially avoids artificial epitope(s)selection In this respect preloading microparticlescontaining antigen might facilitate DC uptake andfavor tolerogenic phenotypes Of note early studiesin which rodents were vaccinated with autoantigen-loaded DCs demonstrated antigen-specific durableand effective tolerance These principles have beenapplied in human trials of T1D42 Advantages mayalso emerge from strategies in which DCs are al-lowed to antigen-load in situ
Small phase I clinical trials exploring DC vaccinesin autoimmune disease have largely focused on T1Dor rheumatoid arthritis Evidence to date suggests thattolerizing DC therapies are safe However larger trialswill be required to more thoroughly assess safety andefficacy Despite the great interest in further assessingDC vaccines in the prevention modulation and cur-ing of autoimmune diseases key questions remainThese include optimization of DC dosing vaccina-tion frequency (eg boosting) route of administra-tion and identification or validation of biomarkersfor assessing therapeutic efficacy
NMOSD appears to represent an ideal disease forDC therapy because a dominant and T cellndashrestrictedautoantigen (AQP4) has been identified If successfulthis approach should be used as early in the diseasecourse as is possible The rationale for this strategy isto prevent or ameliorate the expansion of inflamma-tory AQP4-restricted or other antigen-restrictedT lymphocytes thereby minimizing broader tissueinjury In addition such early intervention could inconcept minimize the potential for antigen spreadingor reversal of antigenic ignorance
Antigen-coupled presentation Antigen-specific toleriza-tion in animal models of disease has been pursued bya number of laboratory groups Among these modelsare those for experimental autoimmune encephalo-myelitis (EAE) Theiler virusndashinduced encephalitistolerance to organ transplantation and therapyfor severe allergy645ndash50 Use of peptide-coupled orchemically fixed antigen-presenting cells (APCs) byMiller et al6 has been one strategy Here unmodifiedsplenocytes are pulsed in vitro with a disease-relevantautoantigen(s) together with a coupling agent Thehost is immunized using these cells with the goal ofinducing tolerance to a target autoantigen(s) Thisapproach was effective in reducing disease activityin a model of relapsing-remitting EAE in SJL
Neurology Neuroimmunology amp Neuroinflammation 5
ordf 2016 American Academy of Neurology Unauthorized reproduction of this article is prohibited
mice4547 The strategy may also be attractive asprophylaxis for allotransplantation and the attenuationof alloreactive immune responses51 Key advantages ofthis approach may include (1) extensive experience inEAE and other autoimmune disease models (2) realisticpotential for targeting multiple autoantigens (3)acceptable safety profiles in preclinical studies (4) anapparent absence of epitope spreading and (5) positivepreliminary evidence for efficacy45 These attributesprovide a strong rationale for applying this approach toindividuals with antigenically characterized autoimmunediseases such as NMOSD
This strategy has been translated to human trialsusing unaltered autologous peripheral blood mono-nuclear cells (PBMCs) as APCs following their isola-tion by therapeutic leukapheresis52 In MS isolatedcells are first pulsed in vitro with a mixture of immu-nodominant myelin peptides that are identified astargets of the high-avidity myelin-specific T cellresponse53 A phase I trial was conducted in whichpatients received graded dosages of peptide-coupledand fixed autologous PBMCs52 Results suggested thisapproach was well tolerated and induced neitherimmune reactivity to myelin peptides nor triggereddisease relapses Rather the data demonstrated thatthe abundance of myelin-reactive T cells was substan-tially reduced52 Several putative mechanisms ofaction have been attributed to this strategy FirstAPC presentation of cross-linked peptide(s) can leadto anergy andor functional silencing of T cells result-ing from inadequate second-signaling4954 Here thedominant mechanisms are most likely related to Tcell recognition of peptide-coupled antigen com-plexes In this scenario T cells andor APCs undergoapoptosis and are phagocytosed by a special subset ofimmature DCs or macrophages in the splenic mar-ginal zone454950 These immature DCsmacrophagescan present an autoantigen peptide(s) in a tolerogenicmanner (eg IL-10) to pathogenic autoreactiveT cells Each of these pathways might help dampenautoimmunity Induction of Tregs is also likely criticalin suppressing autoreactivity45 Tolerization withpeptide-coupled fixed PBMCs undergoing apoptosisin vivo does not lead to the induction of anti-DNAautoantibodies55 The relevant mechanism(s) involvedin this immune tolerizing strategy and how it might beapplied to human diseases require further study
Despite promising results in animals humanstudies have been limited to demonstrating that theabundance of myelin-specific T cells is substantiallyreduced52 however this phase I study was not designedto elucidate specific mechanisms of action The abovetolerization approach with AQP4-coupled fixed APCscould in theory be readily adapted to NMOSDMoreover epitope spreading can occur in this disease56
and may be attenuated by this strategy (reviewed in
reference 57) Thus tolerization with the entireAQP4 holoprotein or derivative immunodominantpeptides may dampen autoreactivity against AQP4and secondary autoantigen targets providing clinicalbenefit Naturally technical challenges are likely to arisein these therapeutic strategies and their approach to theclinic Moreover specific biomarkers for determiningefficacy will be needed to unambiguously inform thetherapeutic benefit Nonetheless this approach holdspromise for restoring immune tolerance in NMOSDand other autoimmune conditions
T cell receptor engineering The ab TcR is the criticalantigen recognition determinant on CD41 and CD81
T cells conferring both antigen specificity and sensitiv-ity TcR diversity results from rearrangement of multi-ple genes (Figure 2) A key difference from antibodies isthe requirement that ab TcRs bind to peptide ligandsdisplayed through the APC MHC This MHC restric-tion focuses T cell activity against cells that bear therespective antigen This mechanism facilitates targetingto antigen-enriched anatomical sites such as lymphnodes and infected tissues while effector T cellspromote immune responses against foreign orendogenous autoantigens CD41 Tregs suppress theseresponses (part II)
Improved understanding of TcR-driven immunerecognition should yield strategies to harness thesecells in treating autoimmune diseases For exampleautoreactive TcR functions may be redirected tomodulate their disease-propagating phenotypes Bycomparison ongoing efforts to generate therapeuticautologous T cells targeting cancer involve engineer-ing TcRs specific to cancer peptideclass I MHC anti-gens58e1 These cells mature into effector T cells exvivo that are then administered to the patient Initialattempts involved CD81 T cell clones exhibiting rel-atively low affinities (KD range 10ndash100 mM)e1 How-ever subsequent engineering has yielded higheraffinity TcRs with KD ranges from 01 to 1 mMMoreover TcRs targeting class I antigens do notrequire the CD8 coreceptor and are thus capable ofrecruiting effector CD41 T cells against cancer cellsexpressing their cognate target antigene2 Conceiv-ably this approach might also prove useful in target-ing autoreactive cells To date TcR engineering hasbeen accomplished using several methodologiesincluding yeast and phage displaye3
Engineered TcRs can be delivered using a varietyof strategies These include administration as solubleagents via gene transfer or as adoptive T cell thera-pies Soluble TcRs will likely require affinities compa-rable to those of antibodies (KD in nM or pM range)and formatting as immunoglobulin fusions bispecificagents with anti-CD3e4 or as fusions with immuno-regulatory molecules such as cytokinese5 A high-
6 Neurology Neuroimmunology amp Neuroinflammation
ordf 2016 American Academy of Neurology Unauthorized reproduction of this article is prohibited
affinity TcR against a class II MHC fused to IL-10has been developed as a means for downregulatingautoimmune responsese6 Optimizing the tissue tar-geting of these constructs remains challenging
Adoptive T cell transfer of enriched Tregs has alsobeen used as a method of antigen-specific toleriza-tione7e8 Yet optimal delivery of antigen-specific sta-ble Tregs remains to be determined Moreover TcRaffinity and specificity of Tregse9 is less well charac-terized than those of effector T cells Several studieshave demonstrated that introducing class IndashrestrictedTcR into Tregs can redirect their specific activitiese10
and that TcRs with KDs of approximately 1 mM maybe of sufficient avidity to drive Treg activitye11 TcRsdirected against class I targets required lower affinitiesthan those in effector CD41 T cells that promoteTreg activity
Opportunities to generate mouse models and ther-apeutic T cells targeting AQP4 would appear to benumerouse12 For example in a mouse model animmunogenic response can be elicited with effectorT cells (CD41 andor CD81) expressing an AQP4-specific antibody (scFv)e13 fused to a chimeric antigenreceptor (CAR) Redirection of Tregs to suppresseffector cells could provide greater therapeutic poten-tial and utilize CARs directed against AQP4 Alter-natively conventional TcRs targeting AQP4 peptideMHC class I complexes such as human leukocyteantigen (HLA)-A2 (which are expressed by 40 ofthe population) might prove effective Possible advan-tages of CARs include their potential use of scFv fromexisting monoclonal antibodiese13 Furthermore thesame scFv used in effector T cells to initiate patho-genic responses could instead be engineered to a Tregphenotype Here scFv affinity could be optimizedusing single-site complementarity determining re-gions mutations Treg signaling and activity woulddepend on the CAR signaling domain specificityand affinity as well as density of AQP4 displayedon target cells Animal models that more preciselyrecapitulate human disease would represent signifi-cant advances toward these goals
The TcR approach offers potential advantage overscFvCAR including direct targeting of Treg activityto class Indashbearing tissues and regional lymph nodeswhere AQP4 might be cross-presented by MHCOstensibly this strategy might arrest or reversecross-presentation of AQP4 antigens by class IIMHC which may occur early in the developmentof NMOSDe14 If so this approach may also mitigateepitope spreading The generation of TcRs againstAQP4 peptides that bind to HLA-A2 would be rela-tively straightforward given the extensive knowledgeof its binding motifs and the likelihood that AQP4contains cognate epitopese15 The TcR affinity in thisscenario may prove inadequate but such limitations
might be overcome through the generation of CARmutants
AQP4 antigenndashspecific approaches must addressissues well beyond TcR and CAR affinity Theseinclude untoward conversion of Tregs to pathogenicphenotypes such as those exhibiting either Th1 orTh17 paradigmse16 accurate tissue targeting and pro-motion of memory and stabilitye17e18 It is possiblethat the same tolerance checkpoints currently beingconsidered for rapid deletion of undesirable T cellstransferred in cancer could be applied to NMOSD
SUMMARY AND PROSPECTUS PART I Immunetolerization attempts to restore central or peripheral tol-erance thereby suppressing autoimmune diseases Thereis reason for optimism that this approach might offersubstantial clinical benefit to individuals with NMOSD DNA vaccination T cell vaccination tolerogenicDCs and peptide-coupling strategies have advancedto clinic trials Other promising approaches (egCAR-based or Treg-based therapeutics) are in earlierdevelopmental stages in autoimmune diseases Yetdefining target cell lineages to yield durable efficacyand determining stage of disease development optimalfor intervention remain crucial ongoing challenges Pastfailures using similar strategies have provided criticalinsights that despite the complexities of existing andemerging technologies for promoting tolerance shouldilluminate the pathway forward If so such advances intreatment could benefit individuals with NMOSDand other autoimmune diseases including arrestingdisease progression prolonging intervals to remissionenabling tissue repair and ultimately avoiding chronicimmunosuppressive therapy
AUTHOR CONTRIBUTIONSLarry Steinman draftingrevising the manuscript Amit Bar-Or drafting
revising the manuscript Jacinta M Behne revising the manuscript
Daniel Benitez-Ribas draftingrevising the manuscript Peter S Chin
draftingrevising the manuscript Michael Clare-Salzler draftingrevising
the manuscript Donald Healey draftingrevising the manuscript James
I Kim draftingrevising the manuscript David M Kranz draftingrevis-
ing the manuscript Andreas Lutterotti draftingrevising the manuscript
Roland Martin draftingrevising the manuscript Sven Schippling draft-
ingrevising the manuscript Pablo Villoslada draftingrevising the man-
uscript Cheng-Hong Wei draftingrevising the manuscript Howard L
Weiner draftingrevising the manuscript Scott S Zamvil draftingrevis-
ing the manuscript Michael R Yeaman draftingrevising the manu-
script study concept or design analysis or interpretation of data
accepts responsibility for conduct of research and will give final approval
Terry J Smith draftingrevising the manuscript study concept or design
analysis or interpretation of data accepts responsibility for conduct of
research and will give final approval
ACKNOWLEDGMENTWe thank Dr Gerald Nepom Dr Philip Bernstein and Dr William St
Clair for their helpful comments
STUDY FUNDINGThis work was supported in part by the Guthy-Jackson Charitable
Foundation
Neurology Neuroimmunology amp Neuroinflammation 7
ordf 2016 American Academy of Neurology Unauthorized reproduction of this article is prohibited
DISCLOSUREL Steinman served on the scientific advisory board for Novartis Recep-
tos Atreca Tolerion Teva received travel funding andor speaker hon-
oraria from Biogen Bayhill Bayer Celgene Receptos is on the editorial
board for Multiple Sclerosis Journal Proceedings of the National Academy of
Science holds a patents for antigen-specific tolerance has a patent pend-
ing for cytokines and type 1 interferons is on the speakers bureau for
EMD Serono received research support from NIH has stock options
and board membership in Tolerion is on the board of directors for
BioAtla A Bar-Or is on the scientific advisory board for Dionix
Receptos-Celgene RocheGenentech Novartis GSK Guthy-Jackson
Greater Good Foundation Immune Tolerance Network received travel
funding andor speaker honoraria from Receptos-Celgene RocheGen-
entech Novartis Sanofi-Genzyme GSK served on the editorial board
for Neurologyreg Clinical and Experimental Neuroimmunology consulted
for DioGenix Receptos-Celgene RocheGenentech Novartis Sanofi-
Genzyme GSK received research support from Novartis Sanofi-
Genzyme JM Behne and D Benitez-Ribas report no disclosures PS
Chin served as the medical director for Genentech and Novartis Pharma-
ceuticals Corp M Clare-Salzler received research support from NIH
NIAID D Healy has a patent pending for the treatment of B cellndash
mediated autoimmunities with T cell vaccination has been CSO for
Opexa Therapeutics received research support from and holds stock
stock options in Opexa Therapeutics JI Kim is an associate editor for
the Journal of Negative Results in Biomedicine is employed by Unum
Therapeutics DM Kranz has patents and patents pending in the areas
of yeast display and T cell receptor engineering and receives license fee
payments from them consulted for AbbVie received research support
from NIH Melanoma Research Alliance A Lutterotti served on the
scientific advisory board for Bayer Biogen Novartis Genzyme received
travel support from European Charcot Foundation Fundacio GAEM
holds a patent with the University of Zurich received research support
from Wyss Translational Center Austrian MS Society R Martin served
on the scientific advisory board for Biogen Merck Serono Teva
Genzyme Sanofi-Aventis CellProtect Neuway received speaker hono-
raria from Biogen Merck Serono Novartis Roche Genzyme holds
a patent for the therapeutic efficacy of anto-CD25 monoclonal antibody
treatment in combination with IFN-b in MS consulted for Myelin
Repair Foundation The Weatherall Institute of Molecular Studies Uni-
versity of Oxford the Hertie Foundation is a member of the Kuratorium
of the Jung Foundation for Science received research support from
Novartis Biogen Swiss National Science Foundation European Union
Seventh Framework Program European Research Council S Schippling
served on the scientific advisory board for Bayer Healthcare Biogen
Merck Serono Novartis Sanofi-Genzyme TEVA received travel fund-
ing andor speaker honoraria from Bayer Healthcare Biogen Merck
Serono Novartis Sanofi-Aventis TEVA is an associate editor for Fron-
tiers in Neurology holds a patent for therapeutic vaccination in PML
using VP1 and Il7 received research support from Sanofi-Genzyme
Novartis University of Zurich Betty and David Koetser Foundation
for Brain Research Swiss Multiple Sclerosis Society P Villoslada received
travel funding andor speaker honoraria from Novartis Roche Genzyme
served as an academic editor for PLoS One served on the editorial board
for Neurology and Therapy Current Treatment Options in Neurology Mul-
tiple Sclerosis and Demyelinating Disorders holds a patent for methylthioa-
denosine for the treatment of MS Agnostic neurotrophic compounds for
the treatment of brain diseases Gene signature pattern as a biomarker for
MS Algorithm for quantifying fractal dimension in brain MRI received
research support from Novartis Roche Genzyme Instituto de Salud
Carlos III European Commission National MS Society Fundacion
Maraton TV3 holds stock or stock options in Bionure Inc Spire Bio-
ventures Mint-Labs C-H Wei reports no disclosures HL Weiner
served on the scientific advisory board for the Guthy-Jackson Charitable
Foundation Teva Pharmaceutical Industries Biogen Idec Novartis
Sanofi-Aventis consulted for Therapix Biogen Novartis Serono Teva
Sanofi received research support from National Multiple Sclerosis Soci-
ety S Zamvil served on the scientific advisory board for BioMS Teva
Pharmaceuticals Eli Lilly and Co Myelin Repair Foundation is deputy
editor for Neurologyreg Neuroimmunology amp Neuroinflammation consulted
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received research support from NIH NMSS Alexander M and June L
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PLoS Pathogens holds patents for Vaccines targeting drug-resistant patho-
gens immunotherapies targeting drug-resistant pathogens novel anti-
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Received February 12 2016 Accepted in final form July 15 2016
REFERENCES1 Levy M Wildemann B Jarius S et al Immunopathogenesis
of neuromyelitis optica Adv Immunol 2014121213ndash242
2 Wingerchuk DM Banwell B Bennett JL et al Interna-
tional consensus diagnostic criteria for neuromyelitis opti-
ca spectrum disorders Neurology 201585177ndash189
3 Lennon VA Kryzer TJ Pittock SJ Verkman AS
Hinson SR IgG marker of optic-spinal multiple sclerosis
binds to the aquaporin-4 water channel J Exp Med 2005
202473ndash477
4 Elsone L Kitley J Luppe S et al Long-term efficacy toler-
ability and retention rate of azathioprine in 103 aquaporin-4
antibody-positive neuromyelitis optica spectrum disorder
patients a multicenter retrospective observational study from
the UK Mult Scler 2014201533ndash1540
5 Nakamura M Nakazawa T Doi H et al Early high-dose
intravenous methylprednisolone is effective in preserving
retinal nerve fiber layer thickness in patients with neuro-
myelitis optica Graefes Arch Clin Exp Ophthalmol 2010
2481777ndash1785
6 Miller SD Wetzig RP Claman HN The induction of
cell-mediated immunity and tolerance with protein anti-
gens coupled to syngeneic lymphoid cells J Exp Med
1979149758ndash773
7 Oh S Schwartz RH Singh NJ Development and toler-
ization of hyperacute rejection in a transgenic mouse graft
versus host model Transplantation 201294234ndash240
8 Castells M Rapid desensitization of hypersensitivity reactions
to chemotherapy agents Curr Drug Saf 20061243ndash251
9 Fousteri G Bresson D von Herrath M Rational develop-
ment of antigen-specific therapies for type 1 diabetes Adv
Exp Med Biol 2007601313ndash319
10 Turley DM Miller SD Prospects for antigen-specific tol-
erance based therapies for the treatment of multiple scle-
rosis Results Probl Cell Differ 201051217ndash235
11 Mori K Yamanishi H Ikeda Y et al Oral administration
of carbonic anhydrase I ameliorates murine experimental
colitis induced by Foxp3-CD41CD252 T cells J Leukoc
Biol 201393963ndash972
12 Genain CP Abel K Belmar N et al Late complications of
immune deviation therapy in a nonhuman primate Sci-
ence 19962742054ndash2057
13 Dolgin E The inverse of immunity Nat Med 201016
740ndash743
14 Steinman L Inverse vaccination the opposite of Jennerrsquos
concept for therapy of autoimmunity J Intern Med 2010
267441ndash451
8 Neurology Neuroimmunology amp Neuroinflammation
ordf 2016 American Academy of Neurology Unauthorized reproduction of this article is prohibited
15 Hinson SR McKeon A Lennon VA Neurological auto-
immunity targeting aquaporin-4 Neuroscience 2010168
1009ndash1018
16 Sato DK Callegaro D de Haidar Jorge FM et al Cere-
brospinal fluid aquaporin-4 antibody levels in neuromye-
litis optica attacks Ann Neurol 201476305ndash309
17 Steinman L The re-emergence of antigen-specific toler-
ance as a potential therapy for MS Mult Scler 201521
1223ndash1238
18 Roep BO Solvason N Gottlieb PA et al Plasmid
encoded proinsulin preserves C-peptide while specifically
reducing proinsulin specific CD8 T cells in type 1 diabe-
tes Sci Transl Med 20135191ra82
19 Feldmann M Steinman L Design of effective immuno-
therapy for human autoimmunity Nature 2005435
612ndash619
20 Garren H Robinson WH Krasulovaacute E et al Phase 2 trial
of a DNA vaccine encoding myelin basic protein for mul-
tiple sclerosis Ann Neurol 200863611ndash620
21 Bar-Or A Vollmer T Antel J et al Induction of antigen-
specific tolerance in multiple sclerosis after immunization
with DNA encoding myelin basic protein in a randomized
placebo-controlled phase 12 trial Arch Neurol 200764
1407ndash1415
22 Selmi C Autoimmunity in 2012 Clin Rev Allergy
Immunol 201345290ndash301
23 Martin A Tisch RM Getts DR Manipulating T cell-
mediated pathology targets and functions of monoclonal
antibody immunotherapy Clin Immunol 2013148
136ndash147
24 Salvana EM Salata RA Infectious complications associ-
ated with monoclonal antibodies and related small mole-
cules Clin Microbiol Rev 200922274ndash290
25 Madakamutil LT Maricic I Sercarz EE Kumar V
Immunodominance in the TCR repertoire of a TCR
peptide-specific CD41 Treg population that controls
experimental autoimmune encephalomyelitis J Immunol
20081804577ndash4585
26 Buckner JH Mechanisms of impaired regulation by CD4
(1)CD25(1)FOXP3(1) regulatory T cells in human
autoimmune diseases Nat Rev Immunol 201010849ndash
859
27 Linhares UC Schiavoni PB Barros PO et al The ex vivo
production of IL-6 and IL-21 by CD41 T cells is directly
associated with neurological disability in neuromyelitis op-
tica patients J Clin Immunol 201333179ndash189
28 Pohl M Fisher MT Mader S et al Pathogenic T cells
responses against aquaporin 4 Acta Neuropathol 2011
12221ndash34
29 Varrin-Doyer M Spencer CM Schulze-Topphoff U et al
Aquaporin 4-specific T cells in neuromyelitis optica
exhibit a Th17 bias and recognize clostridium ABC trans-
porter Ann Neurol 20127253ndash64
30 Kumar V Sercarz EE The involvement of T cell receptor
peptide-specific regulatory CD41 T cells in recovery from
antigen-induced autoimmune disease J Exp Med 1993
178909ndash916
31 Zhang J T cell vaccination as an immunotherapy for
autoimmune diseases Cell Mol Immunol 20041321ndash
327
32 Huang X Wu H Lu Q The mechanisms and applica-
tions of T cell vaccination for autoimmune diseases a com-
prehensive review Clin Rev Allergy Immunol 201447
219ndash233
33 Fontana R Bregni M Cipponi A et al Peripheral blood
lymphocytes genetically modified to express the selftumor
antigen MAGE-A3 induce antitumor immune responses
in cancer patients Blood 20091131651ndash1660
34 Fox E Wynn D Cohan S Rill D McGuire D
Markowitz C A randomized clinical trial of autolo-
gous T-cell therapy in multiple sclerosis subset anal-
ysis and implications for trial design Mult Scler 2012
18843ndash852
35 Chen G Li N Zang YC et al Vaccination with selected
synovial T cells in rheumatoid arthritis Arthritis Rheum
200756453ndash463
36 Li ZG Mu R Dai ZP Gao XM T cell vaccination in
systemic lupus erythematosus with autologous activated T
cells Lupus 200514884ndash889
37 Leavy O Dendritic cells tracing the origins of cDCs Nat
Rev Immunol 201313703
38 Banchereau J Palucka AK Dendritic cells as therapeutic
vaccines against cancer Nat Rev Immunol 20055296ndash
306
39 Vacchelli E Vitale I Eggermont A et al Trial watch
dendritic cell-based interventions for cancer therapy
Oncoimmunology 201211111ndash1134
40 Mantia-Smaldone GM Chu CS A review of dendritic cell
therapy for cancer progress and challenges BioDrugs
201327453ndash468
41 Segovia-Gamboa N Rodriguez-Arellano ME Rangel-
Cruz R et al Tolerogenic dendritic cells induce antigen-
specific hyporesponsiveness in insulin- and glutamic acid
decarboxylase 65-autoreactive T lymphocytes from type 1
diabetic patients Clin Immunol 201415472ndash83
42 Giannoukakis N Phillips B Finegold D Harnaha J
Trucco M Phase I (safety) study of autologous tolerogenic
dendritic cells in type 1 diabetic patients Diabetes Care
2011342026ndash2032
43 Boks MA Kager-Groenland J Haasjes MSP Zwaginga JJ
van Ham SM ten Brinke A IL-10-generated tolerogenic
dendritic cells are optimal for functional regulatory T cell
induction- a comparative study on human clinical-
applicable DC Clin Immunol 2012142332ndash342
44 Saito M Nagasaw M Takada H et al Defective IL-10
signaling in hyper-IgE syndrome results in impaired gen-
eration of tolerogenic dendritic cells and induced regula-
tory T cells J Exp Med 2011208235ndash249
45 Miller SD Turley DM Podojil JR Antigen-specific tol-
erance strategies for the prevention and treatment of auto-
immune disease Nat Rev Immunol 20077665ndash677
46 Karpus WJ Pope JG Peterson JD Dal Canto MC
Miller SD Inhibition of Theilerrsquos virus-mediated demye-
lination by peripheral immune tolerance induction
J Immunol 1995155947ndash957
47 Kennedy KJ Smith WS Miller SD Karpus WJ Induc-
tion of antigen-specific tolerance for the treatment of
ongoing relapsing autoimmune encephalomyelitis a com-
parison between oral and peripheral tolerance J Immunol
19971591036ndash1044
48 Kennedy MK Tan LJ Dal Canto MC et al Inhibition of
murine relapsing experimental autoimmune encephalomy-
elitis by immune tolerance to proteolipid protein and its
encephalitogenic peptides J Immunol 1990144909ndash
915
49 Turley DM Miller SD Peripheral tolerance induction
using ethylenecarbodiimide-fixed APCs uses both direct
and indirect mechanisms of antigen presentation for
Neurology Neuroimmunology amp Neuroinflammation 9
ordf 2016 American Academy of Neurology Unauthorized reproduction of this article is prohibited
prevention of experimental autoimmune encephalomyeli-
tis J Immunol 20071782212ndash2220
50 Getts DR Turley DM Smith CE et al Tolerance
induced by apoptotic antigen-coupled leukocytes is
induced by PD-L11 and IL-10-producing splenic macro-
phages and maintained by T regulatory cells J Immunol
20111872405ndash2417
51 Luo X Pothoven KL McCarthy D et al ECDI-fixed allo-
geneic splenocytes induce donor-specific tolerance for long-
term survival of islet transplants via two distinct mechanisms
Proc Natl Acad Sci USA 200810514527ndash14532
52 Lutterotti A Yousef S Sputtek A et al Antigen-specific
tolerance by autologous myelin peptide-coupled cells
a phase 1 trial in multiple sclerosis Sci Transl Med
20135188ra75
53 Bielekova B SungMH KadomN Simon R McFarland HF
Martin R Expansion and functional relevance of high-avidity
myelin-specific CD41 T cells in multiple sclerosis
J Immunol 20041723893ndash3904
54 Jenkins MK Mueller D Schwartz RH et al Induction
and maintenance of anergy in mature T cells Adv Exp
Med Biol 1991292167ndash176
55 Peschl P Reindl M Schanda K Sospedra M
Martin R Lutterotti A Anti-myelin antibody re-
sponses following induction of antigen-specific toler-
ance with antigen-coupled cells J Neuroimmunol
201427512
56 Tuohy VK Yu M Yin L Kawczak JA Kinkel PR Regres-
sion and spreading of self-recognition during the develop-
ment of autoimmune demyelinating disease J Autoimmun
19991311ndash20
57 Vanderlugt CL Miller SD Epitope spreading in immune-
mediated diseases implications for immunotherapy Nat
Rev Immunol 2002285ndash95
58 Stromnes IM Schmitt TM Chapuis AG Hingorani SR
Greenberg PD Re-adapting T cells for cancer therapy
from mouse models to clinical trials Immunol Rev
2014257145ndash164
10 Neurology Neuroimmunology amp Neuroinflammation
ordf 2016 American Academy of Neurology Unauthorized reproduction of this article is prohibited
DOI 101212NXI000000000000027620163 Neurol Neuroimmunol Neuroinflamm
Larry Steinman Amit Bar-Or Jacinta M Behne et al Restoring immune tolerance in neuromyelitis optica Part I
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This article cites 58 articles 18 of which you can access for free at
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is an official journal of the American Academy of NeurologyNeurol Neuroimmunol Neuroinflamm
Despite substantial advances in understandingNMOSD no existing regimen has been unambigu-ously proven to modify disease course induce perma-nent remission or restore lost neurologic function inadequately powered prospective clinical trials Con-tinuing reliance on nonspecific agents including cor-ticosteroids azathioprine mycophenolate and B celldepletion45 exposes patients to potentially seriousadverse effects Absence of animal models with com-plete fidelity to human NMOSD has been a histori-cal impediment to novel therapies
Improved diagnosis has facilitated greater focuson developing specific therapies Antigen-specific ap-proaches could restore immune tolerance to AQP4and minimize epitope spreading In NMOSD dys-functions in checkpoints governing immune cell devel-opment contribute to autoreactive immunity (figure 2)Considerable progress has been made in tolerizingstrategies used to treat allergies6 transplant and graftrejection7 and drug hypersensitivity8 In theoryantigen-targeted approaches spare normal immunefunctions To date similar strategies have yielded onlylimited success in other autoimmune diseases such astype 1 diabetes (T1D)9 relapsing-remitting MS10 andinflammatory bowel disease11 Results thus far supportthe general concept that inhibition of antigen-specificT cell andor B cell responses is achievable and may bedurable However in some experimental models tol-erization strategies have worsened disease12 perhapsbecause of uncertain disease etiology Hence in thespecific context of NMOSD tolerization might havedistinct advantages
The Guthy-Jackson Charitable Foundation con-vened a meeting of experts in development ofantigen-specific therapy Key goals of this meetingwere to ascertain the state-of-the-art in immune toler-ization as it might be applied to NMOSD and to cat-alyze innovative thinking The following discussionconsiders specific opportunities and challenges toapply tolerization therapy to solve NMOSD
POTENTIAL STRATEGIES FOR TOLERIZATIONIN NMO Inverse DNA vaccination Dolgin13 andSteinman14 have built on principles of classic desen-sitization originating with Jenner While traditionalvaccines induce immune responses to specific anti-gens inverse vaccines promote immune toleranceDiseases in which a known antigen underlies thepathophysiology would in theory represent the mostpromising candidates for tolerizing therapies Thusseropositive NMOSD should be ideal for antigen-specific therapy as autoimmune responses to AQP4are believed to be fundamental to the disease15ndash17 Inclinical trials pathogenic vs tolerogenic immuneresponses of T and B cells would necessarily bemonitored Other diseases with similarly implicatedautoantigens include myasthenia gravis (acetylcholinereceptor) Graves disease (thyrotropin receptor andthyroglobulin) and T1D (multiple insulin granuleantigens)18
Inverse vaccination with DNA encoding proinsu-lin to reinforce tolerogenic immune checkpoints hasbeen evaluated in patients in an early-phase clinicaltrial in T1D1819 The results suggest that immune
Figure 1 Integrative model of NMOSD pathogenesis
Distinct stages of disease are illustrated as relative temporal and spatial comparators in NMOSD progression Note that over the course of disease pro-gression and demyelination additional autoantigens may be revealed andor recognized potentially resulting in antigen spreading and expansioncompound-ing of autoreactivity A 5 astrocyte (AQP4-expressing cells that support neuronal viability and function) AQP4 5 aquaporin 4 B 5 B lymphocyte BBB 5
blood-brain barrier E5 eosinophil M5monocytemacrophage N5 neuron NK5 natural killer cell NMOSD5 neuromyelitis opticaspectrum disorder P5
polymorphonuclear leukocyte (neutrophil) PER 5 periphery T 5 T lymphocyte
2 Neurology Neuroimmunology amp Neuroinflammation
ordf 2016 American Academy of Neurology Unauthorized reproduction of this article is prohibited
tolerance to proinsulin can be induced (p 0026)The study revealed an inverse relationship betweenantigen-restricted CD81 T cells and serum proinsulinlevels18 A similar strategy has enabled proof-of-principleof altered responses to myelin basic protein in patientswith MS2021 Although such approaches are attractiverelevant antigenic targets likely differ among patientsand may change over time For example myelin oligo-dendrocyte glycoprotein may drive unique pathogenesisin NMOSD and require specific tolerization strategiesMoreover epitopes may differ in peptides as comparedto parent proteins and lipid or carbohydrate modifica-tion may influence presentation
Inverse DNA vaccination targeting tolerizationagainst AQP4 immunoreactivity has also been exam-ined in experimental models of NMOSD A plasmidencoding AQP4 attenuated T and B cell responses spe-cifically to this antigen attesting to antigen specificityof this strategy (unpublished observations) In NMOSD clinical trials may be designed based on one ormore diagnostic biomarker(s) of NMO-IgG seroposi-tivity In this approach a diagnostic biomarker(s) isused as a surrogate of therapeutic efficacy and durability
and serves as an outcome measure This scenario ap-proaches an idealized situation in which biomarkersare both the principal diagnostic gating targets andthe major pathogenic factor(s)
Antiautoreactive T cell vaccines Roles played by autor-eactive T cells in mediating autoimmunity have beenestablished in several animal models and comple-mented observations made in human disease22 Inturn therapeutic modulation of autoreactive T cellfrequency andor function in autoimmune diseasesappears achievable23 However regulation of allimmune responses results from the ldquochecks and bal-ancesrdquo imposed by both effector and regulatory T cellnetworks (figure 3) Thus nonspecific depletion ofeffector T cells could diminish immune surveillanceleading to infections or cancer24 Similarly indiscrim-inate activation or depletion of regulatory T cells(Tregs) may also result in immune dysfunction Inparticular Tregs are capable of limiting the frequencyandor activity of individual autoreactive T cell clonesthrough recognition of their unique T cell receptor(TcR) idiotypes25 When T cells aberrantly recognize
Figure 2 Comparative milestones in T and B lymphocyte maturation
(A) T cell development (B) B cell development Each lineage is represented with distinguishing markers for corresponding stages and where cell commitmentoccurs Arrows indicate potential points along the development pathway where immune tolerizing interventions might be targeted in neuromyelitis opticaspectrum disorder CAR5 chimeric antigen receptor CD5 class designation marker CLP5 common lymphoid precursor DN5 double-negative thymocyteDP5 double-positive CD41CD81 thymocyte Ig5 immunoglobulin ImmB5 immature B cell ISP5 immature single-positive CD81 thymocyte LPreB5 largePreB MatB 5mature B cell PreB 5 pre-B cell receptor B cell ProB 5 progenitor B cell SP 5 single positive CD41 or CD81 thymocyte SPreB 5 small PreBTcR 5 T cell receptor Treg 5 regulatory T cell
Neurology Neuroimmunology amp Neuroinflammation 3
ordf 2016 American Academy of Neurology Unauthorized reproduction of this article is prohibited
self-antigens in an absence of adequate regulatorycounterbalance autoimmune disease might result26
In NMOSD the pathogenic antibody response isdominated by class-switched IgG indicating anunderlying AQP4-reactive T cell activity2728 Inaddition Th17 polarization of naive T cells leads tothe secretion of proinflammatory cytokines in relationto target lesions including interleukin (IL)-17A andgranulocyte macrophagendashcolony stimulating factor29
Such a proinflammatory cytokine profile maypromote tissue destruction through recruitment ofgranulocytes or other cells capable of antibody-dependent cell cytotoxicity
The observation that anti-idiotypic Tregs can arisespontaneously and be associated with the resolution ofautoimmunity led to the concept of T cell vaccina-tion3031 This strategy invokes multiple immune func-tions that may mitigate autoimmune disease Forexample induction of idiotype-restricted CD41CD251FoxP31 Tregs IL-10ndashsecreting CD41 Tr1cells and CD81 cytotoxic T cells can result each capableof selectively targeting autoreactive T cells that expressthe idiotype represented by the immunizing T cells32
T cellndashbased vaccines typically use attenuatedautologous autoreactive effector T cells as immuno-gens32 in an amplification strategy designed to targetand counteract autoreactive T cells Theoreticallythis process should selectively deplete andor func-tionally attenuate pathogenic T cells carrying the cor-responding idiotype Restoring an adequate Treg
influence theoretically should mitigate tissue dysfunc-tion Clinical trials have suggested a therapeutic ben-efit from T cell vaccination with minimal sideeffects33 In this regard proof-of-principle has beendemonstrated in several animal models31 In additiontherapeutic trials applying this strategy to MS rheu-matoid arthritis and systemic lupus erythematosusndashassociated nephritis have been undertaken In some ofthese conditions a T cellndashbased vaccine approach hasdemonstrated considerable promise34ndash36
The manufacture of autologous T cell vaccines isdependent first on identifying autoantigen-specificT cells within the peripheral blood of that patientThis goal is achieved by performing an epitope profil-ing assay using overlapping peptides comprising theamino acid sequence of the target antigen The assayreadout is based on the detection of proinflammatorycytokines induced by immunodominant peptideswithin the target antigen This strategy thereby iden-tifies peptides and the associated T cell responsesmost relevant to the autoimmune disease in anaffected individual Next bulk T cell lines areexpanded in vitro from an autologous peripheralblood sample against the selected peptides to providesufficient numbers of autoreactive T cells to meetdosing requirements These particular T cells are ali-quoted and cryopreserved On demand a dose-equivalent is thawed formulated and attenuatedby irradiation before subcutaneous administrationMethods currently in use allow a single-cycle
Figure 3 Hypothesized cellular and molecular crosstalk influencing NMOSD
(A) Theoretical proinflammatory interactions that arise from loss of immune tolerance which may promote the onset or pro-gression of neuromyelitis opticaspectrum disorder (NMOSD) (B) Theoretical anti-inflammatory interactions that arise andcould be amenable to therapeutic intervention in restoring immune tolerance in NMOSD (Cellular) APC5 antigen-present-ing cell Bc5B cell Tc5 T cell (cell surface determinants) CD5 class designation MHC5major histocompatibility complex(soluble) BAFF 5 B cell activating factor IL 5 interleukin IL-18BP 5 IL-18 binding protein sIL-1R 5 soluble IL-1 receptorsTNFR 5 soluble tumor necrosis factor a receptor
4 Neurology Neuroimmunology amp Neuroinflammation
ordf 2016 American Academy of Neurology Unauthorized reproduction of this article is prohibited
manufacturing process from one blood collection tobe used to generate sufficient drug for at least 1 yearof treatment
Considering the aggregate evidence a compellingcase can be made for the critical role of T cells inthe pathogenesis of NMOSD29 It follows thatreestablishment of immune tolerance to AQP4through vaccination with autologous attenuatedand idiotype-restricted T cells represents a promisingnew approach to immunotherapy in this disease
Dendritic cell vaccines Dendritic cells (DCs) presentantigens to immune cells including those with regula-tory and autoreactive function through major histo-compatibility complex (MHC) class I and IIrestriction Normally DCs present self-antigens butdo not drive autoreactive immune responses Thusautoreactive T cells are inactivated or anergizedbecause of low MHC expression inadequatecostimulation (eg inducing anergy) or tolerogeniccostimulation (eg PD-L1 [programed death ligand1] IL-10 etc) In addition DCs have important rolesin maintaining tolerance by promoting regulatorycells such as CD41 and CD81 Tregs and IL-10ndashproducing regulatory B cells37 Immature human DCsare readily generated in the laboratory from bloodmonocytes and can be engineered to maintaintolerogenic phenotypes Indeed DC-based therapieshave now entered clinical trials focusing on NMOSD(ClinicalTrialsgov identifier NCT02283671)
As a strategy for inducing immune tolerance inT1D DC administration has proven relatively safe38ndash42 Here granulocyte macrophagendashcolony stimulatingfactorIL-4 was used to generate immature CD11c1
DCs from monocytes in vitro Transition to a proin-flammatory DC phenotype is concerning when DCsare administered to human patients To mitigate thisconcern immature DCs can be treated in vitro withimmunosuppressive agents such as corticosteroids vita-min D3 andor IL-1043 before their use as tolerizingvaccines The resulting DCs exhibit durable tolerogenicphenotypes that prolong organ allograft survival inmice Notably this conditioning enhances the tolero-genic properties of DCs44 and induces high-level IL-10production which inhibit activated Th1 and Th17cells induce Tregs and suppress autoreactive B cellfunctions A related strategy involves loading of imma-ture DCs with antisense oligonucleotides targeting keycostimulatory molecules such as CD40 CD80 orCD86 Alternatively viral vectors encoding immuno-regulatory factors may potentiate tolerogenic DCs Ofnote the maintenance of DC immaturity also allowsthese cells to migrate into lymph nodes following anti-gen exposure enhancing their tolerogenic potential
Loading immature DCs with either native or syn-thetic peptides or whole antigens may also promote
antigen-specific tolerance through induction of regula-tory lymphocytes This strategy addresses multiple auto-antigens andor epitopes within a target antigen(s)Thus the goal is conditional presentation of self-antigenic epitopes by DCs to yield Treg inductionUsing the entire antigen affords presentation of a broad-er repertoire of peptides selected by relevant MHC al-leles and potentially avoids artificial epitope(s)selection In this respect preloading microparticlescontaining antigen might facilitate DC uptake andfavor tolerogenic phenotypes Of note early studiesin which rodents were vaccinated with autoantigen-loaded DCs demonstrated antigen-specific durableand effective tolerance These principles have beenapplied in human trials of T1D42 Advantages mayalso emerge from strategies in which DCs are al-lowed to antigen-load in situ
Small phase I clinical trials exploring DC vaccinesin autoimmune disease have largely focused on T1Dor rheumatoid arthritis Evidence to date suggests thattolerizing DC therapies are safe However larger trialswill be required to more thoroughly assess safety andefficacy Despite the great interest in further assessingDC vaccines in the prevention modulation and cur-ing of autoimmune diseases key questions remainThese include optimization of DC dosing vaccina-tion frequency (eg boosting) route of administra-tion and identification or validation of biomarkersfor assessing therapeutic efficacy
NMOSD appears to represent an ideal disease forDC therapy because a dominant and T cellndashrestrictedautoantigen (AQP4) has been identified If successfulthis approach should be used as early in the diseasecourse as is possible The rationale for this strategy isto prevent or ameliorate the expansion of inflamma-tory AQP4-restricted or other antigen-restrictedT lymphocytes thereby minimizing broader tissueinjury In addition such early intervention could inconcept minimize the potential for antigen spreadingor reversal of antigenic ignorance
Antigen-coupled presentation Antigen-specific toleriza-tion in animal models of disease has been pursued bya number of laboratory groups Among these modelsare those for experimental autoimmune encephalo-myelitis (EAE) Theiler virusndashinduced encephalitistolerance to organ transplantation and therapyfor severe allergy645ndash50 Use of peptide-coupled orchemically fixed antigen-presenting cells (APCs) byMiller et al6 has been one strategy Here unmodifiedsplenocytes are pulsed in vitro with a disease-relevantautoantigen(s) together with a coupling agent Thehost is immunized using these cells with the goal ofinducing tolerance to a target autoantigen(s) Thisapproach was effective in reducing disease activityin a model of relapsing-remitting EAE in SJL
Neurology Neuroimmunology amp Neuroinflammation 5
ordf 2016 American Academy of Neurology Unauthorized reproduction of this article is prohibited
mice4547 The strategy may also be attractive asprophylaxis for allotransplantation and the attenuationof alloreactive immune responses51 Key advantages ofthis approach may include (1) extensive experience inEAE and other autoimmune disease models (2) realisticpotential for targeting multiple autoantigens (3)acceptable safety profiles in preclinical studies (4) anapparent absence of epitope spreading and (5) positivepreliminary evidence for efficacy45 These attributesprovide a strong rationale for applying this approach toindividuals with antigenically characterized autoimmunediseases such as NMOSD
This strategy has been translated to human trialsusing unaltered autologous peripheral blood mono-nuclear cells (PBMCs) as APCs following their isola-tion by therapeutic leukapheresis52 In MS isolatedcells are first pulsed in vitro with a mixture of immu-nodominant myelin peptides that are identified astargets of the high-avidity myelin-specific T cellresponse53 A phase I trial was conducted in whichpatients received graded dosages of peptide-coupledand fixed autologous PBMCs52 Results suggested thisapproach was well tolerated and induced neitherimmune reactivity to myelin peptides nor triggereddisease relapses Rather the data demonstrated thatthe abundance of myelin-reactive T cells was substan-tially reduced52 Several putative mechanisms ofaction have been attributed to this strategy FirstAPC presentation of cross-linked peptide(s) can leadto anergy andor functional silencing of T cells result-ing from inadequate second-signaling4954 Here thedominant mechanisms are most likely related to Tcell recognition of peptide-coupled antigen com-plexes In this scenario T cells andor APCs undergoapoptosis and are phagocytosed by a special subset ofimmature DCs or macrophages in the splenic mar-ginal zone454950 These immature DCsmacrophagescan present an autoantigen peptide(s) in a tolerogenicmanner (eg IL-10) to pathogenic autoreactiveT cells Each of these pathways might help dampenautoimmunity Induction of Tregs is also likely criticalin suppressing autoreactivity45 Tolerization withpeptide-coupled fixed PBMCs undergoing apoptosisin vivo does not lead to the induction of anti-DNAautoantibodies55 The relevant mechanism(s) involvedin this immune tolerizing strategy and how it might beapplied to human diseases require further study
Despite promising results in animals humanstudies have been limited to demonstrating that theabundance of myelin-specific T cells is substantiallyreduced52 however this phase I study was not designedto elucidate specific mechanisms of action The abovetolerization approach with AQP4-coupled fixed APCscould in theory be readily adapted to NMOSDMoreover epitope spreading can occur in this disease56
and may be attenuated by this strategy (reviewed in
reference 57) Thus tolerization with the entireAQP4 holoprotein or derivative immunodominantpeptides may dampen autoreactivity against AQP4and secondary autoantigen targets providing clinicalbenefit Naturally technical challenges are likely to arisein these therapeutic strategies and their approach to theclinic Moreover specific biomarkers for determiningefficacy will be needed to unambiguously inform thetherapeutic benefit Nonetheless this approach holdspromise for restoring immune tolerance in NMOSDand other autoimmune conditions
T cell receptor engineering The ab TcR is the criticalantigen recognition determinant on CD41 and CD81
T cells conferring both antigen specificity and sensitiv-ity TcR diversity results from rearrangement of multi-ple genes (Figure 2) A key difference from antibodies isthe requirement that ab TcRs bind to peptide ligandsdisplayed through the APC MHC This MHC restric-tion focuses T cell activity against cells that bear therespective antigen This mechanism facilitates targetingto antigen-enriched anatomical sites such as lymphnodes and infected tissues while effector T cellspromote immune responses against foreign orendogenous autoantigens CD41 Tregs suppress theseresponses (part II)
Improved understanding of TcR-driven immunerecognition should yield strategies to harness thesecells in treating autoimmune diseases For exampleautoreactive TcR functions may be redirected tomodulate their disease-propagating phenotypes Bycomparison ongoing efforts to generate therapeuticautologous T cells targeting cancer involve engineer-ing TcRs specific to cancer peptideclass I MHC anti-gens58e1 These cells mature into effector T cells exvivo that are then administered to the patient Initialattempts involved CD81 T cell clones exhibiting rel-atively low affinities (KD range 10ndash100 mM)e1 How-ever subsequent engineering has yielded higheraffinity TcRs with KD ranges from 01 to 1 mMMoreover TcRs targeting class I antigens do notrequire the CD8 coreceptor and are thus capable ofrecruiting effector CD41 T cells against cancer cellsexpressing their cognate target antigene2 Conceiv-ably this approach might also prove useful in target-ing autoreactive cells To date TcR engineering hasbeen accomplished using several methodologiesincluding yeast and phage displaye3
Engineered TcRs can be delivered using a varietyof strategies These include administration as solubleagents via gene transfer or as adoptive T cell thera-pies Soluble TcRs will likely require affinities compa-rable to those of antibodies (KD in nM or pM range)and formatting as immunoglobulin fusions bispecificagents with anti-CD3e4 or as fusions with immuno-regulatory molecules such as cytokinese5 A high-
6 Neurology Neuroimmunology amp Neuroinflammation
ordf 2016 American Academy of Neurology Unauthorized reproduction of this article is prohibited
affinity TcR against a class II MHC fused to IL-10has been developed as a means for downregulatingautoimmune responsese6 Optimizing the tissue tar-geting of these constructs remains challenging
Adoptive T cell transfer of enriched Tregs has alsobeen used as a method of antigen-specific toleriza-tione7e8 Yet optimal delivery of antigen-specific sta-ble Tregs remains to be determined Moreover TcRaffinity and specificity of Tregse9 is less well charac-terized than those of effector T cells Several studieshave demonstrated that introducing class IndashrestrictedTcR into Tregs can redirect their specific activitiese10
and that TcRs with KDs of approximately 1 mM maybe of sufficient avidity to drive Treg activitye11 TcRsdirected against class I targets required lower affinitiesthan those in effector CD41 T cells that promoteTreg activity
Opportunities to generate mouse models and ther-apeutic T cells targeting AQP4 would appear to benumerouse12 For example in a mouse model animmunogenic response can be elicited with effectorT cells (CD41 andor CD81) expressing an AQP4-specific antibody (scFv)e13 fused to a chimeric antigenreceptor (CAR) Redirection of Tregs to suppresseffector cells could provide greater therapeutic poten-tial and utilize CARs directed against AQP4 Alter-natively conventional TcRs targeting AQP4 peptideMHC class I complexes such as human leukocyteantigen (HLA)-A2 (which are expressed by 40 ofthe population) might prove effective Possible advan-tages of CARs include their potential use of scFv fromexisting monoclonal antibodiese13 Furthermore thesame scFv used in effector T cells to initiate patho-genic responses could instead be engineered to a Tregphenotype Here scFv affinity could be optimizedusing single-site complementarity determining re-gions mutations Treg signaling and activity woulddepend on the CAR signaling domain specificityand affinity as well as density of AQP4 displayedon target cells Animal models that more preciselyrecapitulate human disease would represent signifi-cant advances toward these goals
The TcR approach offers potential advantage overscFvCAR including direct targeting of Treg activityto class Indashbearing tissues and regional lymph nodeswhere AQP4 might be cross-presented by MHCOstensibly this strategy might arrest or reversecross-presentation of AQP4 antigens by class IIMHC which may occur early in the developmentof NMOSDe14 If so this approach may also mitigateepitope spreading The generation of TcRs againstAQP4 peptides that bind to HLA-A2 would be rela-tively straightforward given the extensive knowledgeof its binding motifs and the likelihood that AQP4contains cognate epitopese15 The TcR affinity in thisscenario may prove inadequate but such limitations
might be overcome through the generation of CARmutants
AQP4 antigenndashspecific approaches must addressissues well beyond TcR and CAR affinity Theseinclude untoward conversion of Tregs to pathogenicphenotypes such as those exhibiting either Th1 orTh17 paradigmse16 accurate tissue targeting and pro-motion of memory and stabilitye17e18 It is possiblethat the same tolerance checkpoints currently beingconsidered for rapid deletion of undesirable T cellstransferred in cancer could be applied to NMOSD
SUMMARY AND PROSPECTUS PART I Immunetolerization attempts to restore central or peripheral tol-erance thereby suppressing autoimmune diseases Thereis reason for optimism that this approach might offersubstantial clinical benefit to individuals with NMOSD DNA vaccination T cell vaccination tolerogenicDCs and peptide-coupling strategies have advancedto clinic trials Other promising approaches (egCAR-based or Treg-based therapeutics) are in earlierdevelopmental stages in autoimmune diseases Yetdefining target cell lineages to yield durable efficacyand determining stage of disease development optimalfor intervention remain crucial ongoing challenges Pastfailures using similar strategies have provided criticalinsights that despite the complexities of existing andemerging technologies for promoting tolerance shouldilluminate the pathway forward If so such advances intreatment could benefit individuals with NMOSDand other autoimmune diseases including arrestingdisease progression prolonging intervals to remissionenabling tissue repair and ultimately avoiding chronicimmunosuppressive therapy
AUTHOR CONTRIBUTIONSLarry Steinman draftingrevising the manuscript Amit Bar-Or drafting
revising the manuscript Jacinta M Behne revising the manuscript
Daniel Benitez-Ribas draftingrevising the manuscript Peter S Chin
draftingrevising the manuscript Michael Clare-Salzler draftingrevising
the manuscript Donald Healey draftingrevising the manuscript James
I Kim draftingrevising the manuscript David M Kranz draftingrevis-
ing the manuscript Andreas Lutterotti draftingrevising the manuscript
Roland Martin draftingrevising the manuscript Sven Schippling draft-
ingrevising the manuscript Pablo Villoslada draftingrevising the man-
uscript Cheng-Hong Wei draftingrevising the manuscript Howard L
Weiner draftingrevising the manuscript Scott S Zamvil draftingrevis-
ing the manuscript Michael R Yeaman draftingrevising the manu-
script study concept or design analysis or interpretation of data
accepts responsibility for conduct of research and will give final approval
Terry J Smith draftingrevising the manuscript study concept or design
analysis or interpretation of data accepts responsibility for conduct of
research and will give final approval
ACKNOWLEDGMENTWe thank Dr Gerald Nepom Dr Philip Bernstein and Dr William St
Clair for their helpful comments
STUDY FUNDINGThis work was supported in part by the Guthy-Jackson Charitable
Foundation
Neurology Neuroimmunology amp Neuroinflammation 7
ordf 2016 American Academy of Neurology Unauthorized reproduction of this article is prohibited
DISCLOSUREL Steinman served on the scientific advisory board for Novartis Recep-
tos Atreca Tolerion Teva received travel funding andor speaker hon-
oraria from Biogen Bayhill Bayer Celgene Receptos is on the editorial
board for Multiple Sclerosis Journal Proceedings of the National Academy of
Science holds a patents for antigen-specific tolerance has a patent pend-
ing for cytokines and type 1 interferons is on the speakers bureau for
EMD Serono received research support from NIH has stock options
and board membership in Tolerion is on the board of directors for
BioAtla A Bar-Or is on the scientific advisory board for Dionix
Receptos-Celgene RocheGenentech Novartis GSK Guthy-Jackson
Greater Good Foundation Immune Tolerance Network received travel
funding andor speaker honoraria from Receptos-Celgene RocheGen-
entech Novartis Sanofi-Genzyme GSK served on the editorial board
for Neurologyreg Clinical and Experimental Neuroimmunology consulted
for DioGenix Receptos-Celgene RocheGenentech Novartis Sanofi-
Genzyme GSK received research support from Novartis Sanofi-
Genzyme JM Behne and D Benitez-Ribas report no disclosures PS
Chin served as the medical director for Genentech and Novartis Pharma-
ceuticals Corp M Clare-Salzler received research support from NIH
NIAID D Healy has a patent pending for the treatment of B cellndash
mediated autoimmunities with T cell vaccination has been CSO for
Opexa Therapeutics received research support from and holds stock
stock options in Opexa Therapeutics JI Kim is an associate editor for
the Journal of Negative Results in Biomedicine is employed by Unum
Therapeutics DM Kranz has patents and patents pending in the areas
of yeast display and T cell receptor engineering and receives license fee
payments from them consulted for AbbVie received research support
from NIH Melanoma Research Alliance A Lutterotti served on the
scientific advisory board for Bayer Biogen Novartis Genzyme received
travel support from European Charcot Foundation Fundacio GAEM
holds a patent with the University of Zurich received research support
from Wyss Translational Center Austrian MS Society R Martin served
on the scientific advisory board for Biogen Merck Serono Teva
Genzyme Sanofi-Aventis CellProtect Neuway received speaker hono-
raria from Biogen Merck Serono Novartis Roche Genzyme holds
a patent for the therapeutic efficacy of anto-CD25 monoclonal antibody
treatment in combination with IFN-b in MS consulted for Myelin
Repair Foundation The Weatherall Institute of Molecular Studies Uni-
versity of Oxford the Hertie Foundation is a member of the Kuratorium
of the Jung Foundation for Science received research support from
Novartis Biogen Swiss National Science Foundation European Union
Seventh Framework Program European Research Council S Schippling
served on the scientific advisory board for Bayer Healthcare Biogen
Merck Serono Novartis Sanofi-Genzyme TEVA received travel fund-
ing andor speaker honoraria from Bayer Healthcare Biogen Merck
Serono Novartis Sanofi-Aventis TEVA is an associate editor for Fron-
tiers in Neurology holds a patent for therapeutic vaccination in PML
using VP1 and Il7 received research support from Sanofi-Genzyme
Novartis University of Zurich Betty and David Koetser Foundation
for Brain Research Swiss Multiple Sclerosis Society P Villoslada received
travel funding andor speaker honoraria from Novartis Roche Genzyme
served as an academic editor for PLoS One served on the editorial board
for Neurology and Therapy Current Treatment Options in Neurology Mul-
tiple Sclerosis and Demyelinating Disorders holds a patent for methylthioa-
denosine for the treatment of MS Agnostic neurotrophic compounds for
the treatment of brain diseases Gene signature pattern as a biomarker for
MS Algorithm for quantifying fractal dimension in brain MRI received
research support from Novartis Roche Genzyme Instituto de Salud
Carlos III European Commission National MS Society Fundacion
Maraton TV3 holds stock or stock options in Bionure Inc Spire Bio-
ventures Mint-Labs C-H Wei reports no disclosures HL Weiner
served on the scientific advisory board for the Guthy-Jackson Charitable
Foundation Teva Pharmaceutical Industries Biogen Idec Novartis
Sanofi-Aventis consulted for Therapix Biogen Novartis Serono Teva
Sanofi received research support from National Multiple Sclerosis Soci-
ety S Zamvil served on the scientific advisory board for BioMS Teva
Pharmaceuticals Eli Lilly and Co Myelin Repair Foundation is deputy
editor for Neurologyreg Neuroimmunology amp Neuroinflammation consulted
for Biogen Idec Teva Neuroscience EMD Serono Genzyme Novartis
Roche is on the speakers bureau for Advanced Health Media Biogen
received research support from NIH NMSS Alexander M and June L
Maisin Foundation MR Yeaman is on the scientific advisory board for
Guthy-Jackson Charitable Foundation served as an associate editor for
PLoS Pathogens holds patents for Vaccines targeting drug-resistant patho-
gens immunotherapies targeting drug-resistant pathogens novel anti-
infective biological therapeutics novel anti-infective small molecules
novel biological regulating programmed cell death consulted for
Guthy-Jackson Charitable Foundation received research support from
NovaDigm Therapeutics Inc Metacin Inc US Department of Defense
NIH holds stock or stock options for NovaDigm Therapeutics Inc Meta-
cin Inc receives license fee and royalty payments from NovaDigm Thera-
peutics TJ Smith received research support from NIH University of South
Denmark Bell Charitable Foundation RPB Foundation is a member of the
Guthy-Jackson Charitable Foundation scientific advisory board and holds
patents covering the blockade of insulin-like growth factor receptor-l in
autoimmune diseases Go to Neurologyorgnn for full disclosure forms
Received February 12 2016 Accepted in final form July 15 2016
REFERENCES1 Levy M Wildemann B Jarius S et al Immunopathogenesis
of neuromyelitis optica Adv Immunol 2014121213ndash242
2 Wingerchuk DM Banwell B Bennett JL et al Interna-
tional consensus diagnostic criteria for neuromyelitis opti-
ca spectrum disorders Neurology 201585177ndash189
3 Lennon VA Kryzer TJ Pittock SJ Verkman AS
Hinson SR IgG marker of optic-spinal multiple sclerosis
binds to the aquaporin-4 water channel J Exp Med 2005
202473ndash477
4 Elsone L Kitley J Luppe S et al Long-term efficacy toler-
ability and retention rate of azathioprine in 103 aquaporin-4
antibody-positive neuromyelitis optica spectrum disorder
patients a multicenter retrospective observational study from
the UK Mult Scler 2014201533ndash1540
5 Nakamura M Nakazawa T Doi H et al Early high-dose
intravenous methylprednisolone is effective in preserving
retinal nerve fiber layer thickness in patients with neuro-
myelitis optica Graefes Arch Clin Exp Ophthalmol 2010
2481777ndash1785
6 Miller SD Wetzig RP Claman HN The induction of
cell-mediated immunity and tolerance with protein anti-
gens coupled to syngeneic lymphoid cells J Exp Med
1979149758ndash773
7 Oh S Schwartz RH Singh NJ Development and toler-
ization of hyperacute rejection in a transgenic mouse graft
versus host model Transplantation 201294234ndash240
8 Castells M Rapid desensitization of hypersensitivity reactions
to chemotherapy agents Curr Drug Saf 20061243ndash251
9 Fousteri G Bresson D von Herrath M Rational develop-
ment of antigen-specific therapies for type 1 diabetes Adv
Exp Med Biol 2007601313ndash319
10 Turley DM Miller SD Prospects for antigen-specific tol-
erance based therapies for the treatment of multiple scle-
rosis Results Probl Cell Differ 201051217ndash235
11 Mori K Yamanishi H Ikeda Y et al Oral administration
of carbonic anhydrase I ameliorates murine experimental
colitis induced by Foxp3-CD41CD252 T cells J Leukoc
Biol 201393963ndash972
12 Genain CP Abel K Belmar N et al Late complications of
immune deviation therapy in a nonhuman primate Sci-
ence 19962742054ndash2057
13 Dolgin E The inverse of immunity Nat Med 201016
740ndash743
14 Steinman L Inverse vaccination the opposite of Jennerrsquos
concept for therapy of autoimmunity J Intern Med 2010
267441ndash451
8 Neurology Neuroimmunology amp Neuroinflammation
ordf 2016 American Academy of Neurology Unauthorized reproduction of this article is prohibited
15 Hinson SR McKeon A Lennon VA Neurological auto-
immunity targeting aquaporin-4 Neuroscience 2010168
1009ndash1018
16 Sato DK Callegaro D de Haidar Jorge FM et al Cere-
brospinal fluid aquaporin-4 antibody levels in neuromye-
litis optica attacks Ann Neurol 201476305ndash309
17 Steinman L The re-emergence of antigen-specific toler-
ance as a potential therapy for MS Mult Scler 201521
1223ndash1238
18 Roep BO Solvason N Gottlieb PA et al Plasmid
encoded proinsulin preserves C-peptide while specifically
reducing proinsulin specific CD8 T cells in type 1 diabe-
tes Sci Transl Med 20135191ra82
19 Feldmann M Steinman L Design of effective immuno-
therapy for human autoimmunity Nature 2005435
612ndash619
20 Garren H Robinson WH Krasulovaacute E et al Phase 2 trial
of a DNA vaccine encoding myelin basic protein for mul-
tiple sclerosis Ann Neurol 200863611ndash620
21 Bar-Or A Vollmer T Antel J et al Induction of antigen-
specific tolerance in multiple sclerosis after immunization
with DNA encoding myelin basic protein in a randomized
placebo-controlled phase 12 trial Arch Neurol 200764
1407ndash1415
22 Selmi C Autoimmunity in 2012 Clin Rev Allergy
Immunol 201345290ndash301
23 Martin A Tisch RM Getts DR Manipulating T cell-
mediated pathology targets and functions of monoclonal
antibody immunotherapy Clin Immunol 2013148
136ndash147
24 Salvana EM Salata RA Infectious complications associ-
ated with monoclonal antibodies and related small mole-
cules Clin Microbiol Rev 200922274ndash290
25 Madakamutil LT Maricic I Sercarz EE Kumar V
Immunodominance in the TCR repertoire of a TCR
peptide-specific CD41 Treg population that controls
experimental autoimmune encephalomyelitis J Immunol
20081804577ndash4585
26 Buckner JH Mechanisms of impaired regulation by CD4
(1)CD25(1)FOXP3(1) regulatory T cells in human
autoimmune diseases Nat Rev Immunol 201010849ndash
859
27 Linhares UC Schiavoni PB Barros PO et al The ex vivo
production of IL-6 and IL-21 by CD41 T cells is directly
associated with neurological disability in neuromyelitis op-
tica patients J Clin Immunol 201333179ndash189
28 Pohl M Fisher MT Mader S et al Pathogenic T cells
responses against aquaporin 4 Acta Neuropathol 2011
12221ndash34
29 Varrin-Doyer M Spencer CM Schulze-Topphoff U et al
Aquaporin 4-specific T cells in neuromyelitis optica
exhibit a Th17 bias and recognize clostridium ABC trans-
porter Ann Neurol 20127253ndash64
30 Kumar V Sercarz EE The involvement of T cell receptor
peptide-specific regulatory CD41 T cells in recovery from
antigen-induced autoimmune disease J Exp Med 1993
178909ndash916
31 Zhang J T cell vaccination as an immunotherapy for
autoimmune diseases Cell Mol Immunol 20041321ndash
327
32 Huang X Wu H Lu Q The mechanisms and applica-
tions of T cell vaccination for autoimmune diseases a com-
prehensive review Clin Rev Allergy Immunol 201447
219ndash233
33 Fontana R Bregni M Cipponi A et al Peripheral blood
lymphocytes genetically modified to express the selftumor
antigen MAGE-A3 induce antitumor immune responses
in cancer patients Blood 20091131651ndash1660
34 Fox E Wynn D Cohan S Rill D McGuire D
Markowitz C A randomized clinical trial of autolo-
gous T-cell therapy in multiple sclerosis subset anal-
ysis and implications for trial design Mult Scler 2012
18843ndash852
35 Chen G Li N Zang YC et al Vaccination with selected
synovial T cells in rheumatoid arthritis Arthritis Rheum
200756453ndash463
36 Li ZG Mu R Dai ZP Gao XM T cell vaccination in
systemic lupus erythematosus with autologous activated T
cells Lupus 200514884ndash889
37 Leavy O Dendritic cells tracing the origins of cDCs Nat
Rev Immunol 201313703
38 Banchereau J Palucka AK Dendritic cells as therapeutic
vaccines against cancer Nat Rev Immunol 20055296ndash
306
39 Vacchelli E Vitale I Eggermont A et al Trial watch
dendritic cell-based interventions for cancer therapy
Oncoimmunology 201211111ndash1134
40 Mantia-Smaldone GM Chu CS A review of dendritic cell
therapy for cancer progress and challenges BioDrugs
201327453ndash468
41 Segovia-Gamboa N Rodriguez-Arellano ME Rangel-
Cruz R et al Tolerogenic dendritic cells induce antigen-
specific hyporesponsiveness in insulin- and glutamic acid
decarboxylase 65-autoreactive T lymphocytes from type 1
diabetic patients Clin Immunol 201415472ndash83
42 Giannoukakis N Phillips B Finegold D Harnaha J
Trucco M Phase I (safety) study of autologous tolerogenic
dendritic cells in type 1 diabetic patients Diabetes Care
2011342026ndash2032
43 Boks MA Kager-Groenland J Haasjes MSP Zwaginga JJ
van Ham SM ten Brinke A IL-10-generated tolerogenic
dendritic cells are optimal for functional regulatory T cell
induction- a comparative study on human clinical-
applicable DC Clin Immunol 2012142332ndash342
44 Saito M Nagasaw M Takada H et al Defective IL-10
signaling in hyper-IgE syndrome results in impaired gen-
eration of tolerogenic dendritic cells and induced regula-
tory T cells J Exp Med 2011208235ndash249
45 Miller SD Turley DM Podojil JR Antigen-specific tol-
erance strategies for the prevention and treatment of auto-
immune disease Nat Rev Immunol 20077665ndash677
46 Karpus WJ Pope JG Peterson JD Dal Canto MC
Miller SD Inhibition of Theilerrsquos virus-mediated demye-
lination by peripheral immune tolerance induction
J Immunol 1995155947ndash957
47 Kennedy KJ Smith WS Miller SD Karpus WJ Induc-
tion of antigen-specific tolerance for the treatment of
ongoing relapsing autoimmune encephalomyelitis a com-
parison between oral and peripheral tolerance J Immunol
19971591036ndash1044
48 Kennedy MK Tan LJ Dal Canto MC et al Inhibition of
murine relapsing experimental autoimmune encephalomy-
elitis by immune tolerance to proteolipid protein and its
encephalitogenic peptides J Immunol 1990144909ndash
915
49 Turley DM Miller SD Peripheral tolerance induction
using ethylenecarbodiimide-fixed APCs uses both direct
and indirect mechanisms of antigen presentation for
Neurology Neuroimmunology amp Neuroinflammation 9
ordf 2016 American Academy of Neurology Unauthorized reproduction of this article is prohibited
prevention of experimental autoimmune encephalomyeli-
tis J Immunol 20071782212ndash2220
50 Getts DR Turley DM Smith CE et al Tolerance
induced by apoptotic antigen-coupled leukocytes is
induced by PD-L11 and IL-10-producing splenic macro-
phages and maintained by T regulatory cells J Immunol
20111872405ndash2417
51 Luo X Pothoven KL McCarthy D et al ECDI-fixed allo-
geneic splenocytes induce donor-specific tolerance for long-
term survival of islet transplants via two distinct mechanisms
Proc Natl Acad Sci USA 200810514527ndash14532
52 Lutterotti A Yousef S Sputtek A et al Antigen-specific
tolerance by autologous myelin peptide-coupled cells
a phase 1 trial in multiple sclerosis Sci Transl Med
20135188ra75
53 Bielekova B SungMH KadomN Simon R McFarland HF
Martin R Expansion and functional relevance of high-avidity
myelin-specific CD41 T cells in multiple sclerosis
J Immunol 20041723893ndash3904
54 Jenkins MK Mueller D Schwartz RH et al Induction
and maintenance of anergy in mature T cells Adv Exp
Med Biol 1991292167ndash176
55 Peschl P Reindl M Schanda K Sospedra M
Martin R Lutterotti A Anti-myelin antibody re-
sponses following induction of antigen-specific toler-
ance with antigen-coupled cells J Neuroimmunol
201427512
56 Tuohy VK Yu M Yin L Kawczak JA Kinkel PR Regres-
sion and spreading of self-recognition during the develop-
ment of autoimmune demyelinating disease J Autoimmun
19991311ndash20
57 Vanderlugt CL Miller SD Epitope spreading in immune-
mediated diseases implications for immunotherapy Nat
Rev Immunol 2002285ndash95
58 Stromnes IM Schmitt TM Chapuis AG Hingorani SR
Greenberg PD Re-adapting T cells for cancer therapy
from mouse models to clinical trials Immunol Rev
2014257145ndash164
10 Neurology Neuroimmunology amp Neuroinflammation
ordf 2016 American Academy of Neurology Unauthorized reproduction of this article is prohibited
DOI 101212NXI000000000000027620163 Neurol Neuroimmunol Neuroinflamm
Larry Steinman Amit Bar-Or Jacinta M Behne et al Restoring immune tolerance in neuromyelitis optica Part I
This information is current as of September 7 2016
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This article cites 58 articles 18 of which you can access for free at
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is an official journal of the American Academy of NeurologyNeurol Neuroimmunol Neuroinflamm
tolerance to proinsulin can be induced (p 0026)The study revealed an inverse relationship betweenantigen-restricted CD81 T cells and serum proinsulinlevels18 A similar strategy has enabled proof-of-principleof altered responses to myelin basic protein in patientswith MS2021 Although such approaches are attractiverelevant antigenic targets likely differ among patientsand may change over time For example myelin oligo-dendrocyte glycoprotein may drive unique pathogenesisin NMOSD and require specific tolerization strategiesMoreover epitopes may differ in peptides as comparedto parent proteins and lipid or carbohydrate modifica-tion may influence presentation
Inverse DNA vaccination targeting tolerizationagainst AQP4 immunoreactivity has also been exam-ined in experimental models of NMOSD A plasmidencoding AQP4 attenuated T and B cell responses spe-cifically to this antigen attesting to antigen specificityof this strategy (unpublished observations) In NMOSD clinical trials may be designed based on one ormore diagnostic biomarker(s) of NMO-IgG seroposi-tivity In this approach a diagnostic biomarker(s) isused as a surrogate of therapeutic efficacy and durability
and serves as an outcome measure This scenario ap-proaches an idealized situation in which biomarkersare both the principal diagnostic gating targets andthe major pathogenic factor(s)
Antiautoreactive T cell vaccines Roles played by autor-eactive T cells in mediating autoimmunity have beenestablished in several animal models and comple-mented observations made in human disease22 Inturn therapeutic modulation of autoreactive T cellfrequency andor function in autoimmune diseasesappears achievable23 However regulation of allimmune responses results from the ldquochecks and bal-ancesrdquo imposed by both effector and regulatory T cellnetworks (figure 3) Thus nonspecific depletion ofeffector T cells could diminish immune surveillanceleading to infections or cancer24 Similarly indiscrim-inate activation or depletion of regulatory T cells(Tregs) may also result in immune dysfunction Inparticular Tregs are capable of limiting the frequencyandor activity of individual autoreactive T cell clonesthrough recognition of their unique T cell receptor(TcR) idiotypes25 When T cells aberrantly recognize
Figure 2 Comparative milestones in T and B lymphocyte maturation
(A) T cell development (B) B cell development Each lineage is represented with distinguishing markers for corresponding stages and where cell commitmentoccurs Arrows indicate potential points along the development pathway where immune tolerizing interventions might be targeted in neuromyelitis opticaspectrum disorder CAR5 chimeric antigen receptor CD5 class designation marker CLP5 common lymphoid precursor DN5 double-negative thymocyteDP5 double-positive CD41CD81 thymocyte Ig5 immunoglobulin ImmB5 immature B cell ISP5 immature single-positive CD81 thymocyte LPreB5 largePreB MatB 5mature B cell PreB 5 pre-B cell receptor B cell ProB 5 progenitor B cell SP 5 single positive CD41 or CD81 thymocyte SPreB 5 small PreBTcR 5 T cell receptor Treg 5 regulatory T cell
Neurology Neuroimmunology amp Neuroinflammation 3
ordf 2016 American Academy of Neurology Unauthorized reproduction of this article is prohibited
self-antigens in an absence of adequate regulatorycounterbalance autoimmune disease might result26
In NMOSD the pathogenic antibody response isdominated by class-switched IgG indicating anunderlying AQP4-reactive T cell activity2728 Inaddition Th17 polarization of naive T cells leads tothe secretion of proinflammatory cytokines in relationto target lesions including interleukin (IL)-17A andgranulocyte macrophagendashcolony stimulating factor29
Such a proinflammatory cytokine profile maypromote tissue destruction through recruitment ofgranulocytes or other cells capable of antibody-dependent cell cytotoxicity
The observation that anti-idiotypic Tregs can arisespontaneously and be associated with the resolution ofautoimmunity led to the concept of T cell vaccina-tion3031 This strategy invokes multiple immune func-tions that may mitigate autoimmune disease Forexample induction of idiotype-restricted CD41CD251FoxP31 Tregs IL-10ndashsecreting CD41 Tr1cells and CD81 cytotoxic T cells can result each capableof selectively targeting autoreactive T cells that expressthe idiotype represented by the immunizing T cells32
T cellndashbased vaccines typically use attenuatedautologous autoreactive effector T cells as immuno-gens32 in an amplification strategy designed to targetand counteract autoreactive T cells Theoreticallythis process should selectively deplete andor func-tionally attenuate pathogenic T cells carrying the cor-responding idiotype Restoring an adequate Treg
influence theoretically should mitigate tissue dysfunc-tion Clinical trials have suggested a therapeutic ben-efit from T cell vaccination with minimal sideeffects33 In this regard proof-of-principle has beendemonstrated in several animal models31 In additiontherapeutic trials applying this strategy to MS rheu-matoid arthritis and systemic lupus erythematosusndashassociated nephritis have been undertaken In some ofthese conditions a T cellndashbased vaccine approach hasdemonstrated considerable promise34ndash36
The manufacture of autologous T cell vaccines isdependent first on identifying autoantigen-specificT cells within the peripheral blood of that patientThis goal is achieved by performing an epitope profil-ing assay using overlapping peptides comprising theamino acid sequence of the target antigen The assayreadout is based on the detection of proinflammatorycytokines induced by immunodominant peptideswithin the target antigen This strategy thereby iden-tifies peptides and the associated T cell responsesmost relevant to the autoimmune disease in anaffected individual Next bulk T cell lines areexpanded in vitro from an autologous peripheralblood sample against the selected peptides to providesufficient numbers of autoreactive T cells to meetdosing requirements These particular T cells are ali-quoted and cryopreserved On demand a dose-equivalent is thawed formulated and attenuatedby irradiation before subcutaneous administrationMethods currently in use allow a single-cycle
Figure 3 Hypothesized cellular and molecular crosstalk influencing NMOSD
(A) Theoretical proinflammatory interactions that arise from loss of immune tolerance which may promote the onset or pro-gression of neuromyelitis opticaspectrum disorder (NMOSD) (B) Theoretical anti-inflammatory interactions that arise andcould be amenable to therapeutic intervention in restoring immune tolerance in NMOSD (Cellular) APC5 antigen-present-ing cell Bc5B cell Tc5 T cell (cell surface determinants) CD5 class designation MHC5major histocompatibility complex(soluble) BAFF 5 B cell activating factor IL 5 interleukin IL-18BP 5 IL-18 binding protein sIL-1R 5 soluble IL-1 receptorsTNFR 5 soluble tumor necrosis factor a receptor
4 Neurology Neuroimmunology amp Neuroinflammation
ordf 2016 American Academy of Neurology Unauthorized reproduction of this article is prohibited
manufacturing process from one blood collection tobe used to generate sufficient drug for at least 1 yearof treatment
Considering the aggregate evidence a compellingcase can be made for the critical role of T cells inthe pathogenesis of NMOSD29 It follows thatreestablishment of immune tolerance to AQP4through vaccination with autologous attenuatedand idiotype-restricted T cells represents a promisingnew approach to immunotherapy in this disease
Dendritic cell vaccines Dendritic cells (DCs) presentantigens to immune cells including those with regula-tory and autoreactive function through major histo-compatibility complex (MHC) class I and IIrestriction Normally DCs present self-antigens butdo not drive autoreactive immune responses Thusautoreactive T cells are inactivated or anergizedbecause of low MHC expression inadequatecostimulation (eg inducing anergy) or tolerogeniccostimulation (eg PD-L1 [programed death ligand1] IL-10 etc) In addition DCs have important rolesin maintaining tolerance by promoting regulatorycells such as CD41 and CD81 Tregs and IL-10ndashproducing regulatory B cells37 Immature human DCsare readily generated in the laboratory from bloodmonocytes and can be engineered to maintaintolerogenic phenotypes Indeed DC-based therapieshave now entered clinical trials focusing on NMOSD(ClinicalTrialsgov identifier NCT02283671)
As a strategy for inducing immune tolerance inT1D DC administration has proven relatively safe38ndash42 Here granulocyte macrophagendashcolony stimulatingfactorIL-4 was used to generate immature CD11c1
DCs from monocytes in vitro Transition to a proin-flammatory DC phenotype is concerning when DCsare administered to human patients To mitigate thisconcern immature DCs can be treated in vitro withimmunosuppressive agents such as corticosteroids vita-min D3 andor IL-1043 before their use as tolerizingvaccines The resulting DCs exhibit durable tolerogenicphenotypes that prolong organ allograft survival inmice Notably this conditioning enhances the tolero-genic properties of DCs44 and induces high-level IL-10production which inhibit activated Th1 and Th17cells induce Tregs and suppress autoreactive B cellfunctions A related strategy involves loading of imma-ture DCs with antisense oligonucleotides targeting keycostimulatory molecules such as CD40 CD80 orCD86 Alternatively viral vectors encoding immuno-regulatory factors may potentiate tolerogenic DCs Ofnote the maintenance of DC immaturity also allowsthese cells to migrate into lymph nodes following anti-gen exposure enhancing their tolerogenic potential
Loading immature DCs with either native or syn-thetic peptides or whole antigens may also promote
antigen-specific tolerance through induction of regula-tory lymphocytes This strategy addresses multiple auto-antigens andor epitopes within a target antigen(s)Thus the goal is conditional presentation of self-antigenic epitopes by DCs to yield Treg inductionUsing the entire antigen affords presentation of a broad-er repertoire of peptides selected by relevant MHC al-leles and potentially avoids artificial epitope(s)selection In this respect preloading microparticlescontaining antigen might facilitate DC uptake andfavor tolerogenic phenotypes Of note early studiesin which rodents were vaccinated with autoantigen-loaded DCs demonstrated antigen-specific durableand effective tolerance These principles have beenapplied in human trials of T1D42 Advantages mayalso emerge from strategies in which DCs are al-lowed to antigen-load in situ
Small phase I clinical trials exploring DC vaccinesin autoimmune disease have largely focused on T1Dor rheumatoid arthritis Evidence to date suggests thattolerizing DC therapies are safe However larger trialswill be required to more thoroughly assess safety andefficacy Despite the great interest in further assessingDC vaccines in the prevention modulation and cur-ing of autoimmune diseases key questions remainThese include optimization of DC dosing vaccina-tion frequency (eg boosting) route of administra-tion and identification or validation of biomarkersfor assessing therapeutic efficacy
NMOSD appears to represent an ideal disease forDC therapy because a dominant and T cellndashrestrictedautoantigen (AQP4) has been identified If successfulthis approach should be used as early in the diseasecourse as is possible The rationale for this strategy isto prevent or ameliorate the expansion of inflamma-tory AQP4-restricted or other antigen-restrictedT lymphocytes thereby minimizing broader tissueinjury In addition such early intervention could inconcept minimize the potential for antigen spreadingor reversal of antigenic ignorance
Antigen-coupled presentation Antigen-specific toleriza-tion in animal models of disease has been pursued bya number of laboratory groups Among these modelsare those for experimental autoimmune encephalo-myelitis (EAE) Theiler virusndashinduced encephalitistolerance to organ transplantation and therapyfor severe allergy645ndash50 Use of peptide-coupled orchemically fixed antigen-presenting cells (APCs) byMiller et al6 has been one strategy Here unmodifiedsplenocytes are pulsed in vitro with a disease-relevantautoantigen(s) together with a coupling agent Thehost is immunized using these cells with the goal ofinducing tolerance to a target autoantigen(s) Thisapproach was effective in reducing disease activityin a model of relapsing-remitting EAE in SJL
Neurology Neuroimmunology amp Neuroinflammation 5
ordf 2016 American Academy of Neurology Unauthorized reproduction of this article is prohibited
mice4547 The strategy may also be attractive asprophylaxis for allotransplantation and the attenuationof alloreactive immune responses51 Key advantages ofthis approach may include (1) extensive experience inEAE and other autoimmune disease models (2) realisticpotential for targeting multiple autoantigens (3)acceptable safety profiles in preclinical studies (4) anapparent absence of epitope spreading and (5) positivepreliminary evidence for efficacy45 These attributesprovide a strong rationale for applying this approach toindividuals with antigenically characterized autoimmunediseases such as NMOSD
This strategy has been translated to human trialsusing unaltered autologous peripheral blood mono-nuclear cells (PBMCs) as APCs following their isola-tion by therapeutic leukapheresis52 In MS isolatedcells are first pulsed in vitro with a mixture of immu-nodominant myelin peptides that are identified astargets of the high-avidity myelin-specific T cellresponse53 A phase I trial was conducted in whichpatients received graded dosages of peptide-coupledand fixed autologous PBMCs52 Results suggested thisapproach was well tolerated and induced neitherimmune reactivity to myelin peptides nor triggereddisease relapses Rather the data demonstrated thatthe abundance of myelin-reactive T cells was substan-tially reduced52 Several putative mechanisms ofaction have been attributed to this strategy FirstAPC presentation of cross-linked peptide(s) can leadto anergy andor functional silencing of T cells result-ing from inadequate second-signaling4954 Here thedominant mechanisms are most likely related to Tcell recognition of peptide-coupled antigen com-plexes In this scenario T cells andor APCs undergoapoptosis and are phagocytosed by a special subset ofimmature DCs or macrophages in the splenic mar-ginal zone454950 These immature DCsmacrophagescan present an autoantigen peptide(s) in a tolerogenicmanner (eg IL-10) to pathogenic autoreactiveT cells Each of these pathways might help dampenautoimmunity Induction of Tregs is also likely criticalin suppressing autoreactivity45 Tolerization withpeptide-coupled fixed PBMCs undergoing apoptosisin vivo does not lead to the induction of anti-DNAautoantibodies55 The relevant mechanism(s) involvedin this immune tolerizing strategy and how it might beapplied to human diseases require further study
Despite promising results in animals humanstudies have been limited to demonstrating that theabundance of myelin-specific T cells is substantiallyreduced52 however this phase I study was not designedto elucidate specific mechanisms of action The abovetolerization approach with AQP4-coupled fixed APCscould in theory be readily adapted to NMOSDMoreover epitope spreading can occur in this disease56
and may be attenuated by this strategy (reviewed in
reference 57) Thus tolerization with the entireAQP4 holoprotein or derivative immunodominantpeptides may dampen autoreactivity against AQP4and secondary autoantigen targets providing clinicalbenefit Naturally technical challenges are likely to arisein these therapeutic strategies and their approach to theclinic Moreover specific biomarkers for determiningefficacy will be needed to unambiguously inform thetherapeutic benefit Nonetheless this approach holdspromise for restoring immune tolerance in NMOSDand other autoimmune conditions
T cell receptor engineering The ab TcR is the criticalantigen recognition determinant on CD41 and CD81
T cells conferring both antigen specificity and sensitiv-ity TcR diversity results from rearrangement of multi-ple genes (Figure 2) A key difference from antibodies isthe requirement that ab TcRs bind to peptide ligandsdisplayed through the APC MHC This MHC restric-tion focuses T cell activity against cells that bear therespective antigen This mechanism facilitates targetingto antigen-enriched anatomical sites such as lymphnodes and infected tissues while effector T cellspromote immune responses against foreign orendogenous autoantigens CD41 Tregs suppress theseresponses (part II)
Improved understanding of TcR-driven immunerecognition should yield strategies to harness thesecells in treating autoimmune diseases For exampleautoreactive TcR functions may be redirected tomodulate their disease-propagating phenotypes Bycomparison ongoing efforts to generate therapeuticautologous T cells targeting cancer involve engineer-ing TcRs specific to cancer peptideclass I MHC anti-gens58e1 These cells mature into effector T cells exvivo that are then administered to the patient Initialattempts involved CD81 T cell clones exhibiting rel-atively low affinities (KD range 10ndash100 mM)e1 How-ever subsequent engineering has yielded higheraffinity TcRs with KD ranges from 01 to 1 mMMoreover TcRs targeting class I antigens do notrequire the CD8 coreceptor and are thus capable ofrecruiting effector CD41 T cells against cancer cellsexpressing their cognate target antigene2 Conceiv-ably this approach might also prove useful in target-ing autoreactive cells To date TcR engineering hasbeen accomplished using several methodologiesincluding yeast and phage displaye3
Engineered TcRs can be delivered using a varietyof strategies These include administration as solubleagents via gene transfer or as adoptive T cell thera-pies Soluble TcRs will likely require affinities compa-rable to those of antibodies (KD in nM or pM range)and formatting as immunoglobulin fusions bispecificagents with anti-CD3e4 or as fusions with immuno-regulatory molecules such as cytokinese5 A high-
6 Neurology Neuroimmunology amp Neuroinflammation
ordf 2016 American Academy of Neurology Unauthorized reproduction of this article is prohibited
affinity TcR against a class II MHC fused to IL-10has been developed as a means for downregulatingautoimmune responsese6 Optimizing the tissue tar-geting of these constructs remains challenging
Adoptive T cell transfer of enriched Tregs has alsobeen used as a method of antigen-specific toleriza-tione7e8 Yet optimal delivery of antigen-specific sta-ble Tregs remains to be determined Moreover TcRaffinity and specificity of Tregse9 is less well charac-terized than those of effector T cells Several studieshave demonstrated that introducing class IndashrestrictedTcR into Tregs can redirect their specific activitiese10
and that TcRs with KDs of approximately 1 mM maybe of sufficient avidity to drive Treg activitye11 TcRsdirected against class I targets required lower affinitiesthan those in effector CD41 T cells that promoteTreg activity
Opportunities to generate mouse models and ther-apeutic T cells targeting AQP4 would appear to benumerouse12 For example in a mouse model animmunogenic response can be elicited with effectorT cells (CD41 andor CD81) expressing an AQP4-specific antibody (scFv)e13 fused to a chimeric antigenreceptor (CAR) Redirection of Tregs to suppresseffector cells could provide greater therapeutic poten-tial and utilize CARs directed against AQP4 Alter-natively conventional TcRs targeting AQP4 peptideMHC class I complexes such as human leukocyteantigen (HLA)-A2 (which are expressed by 40 ofthe population) might prove effective Possible advan-tages of CARs include their potential use of scFv fromexisting monoclonal antibodiese13 Furthermore thesame scFv used in effector T cells to initiate patho-genic responses could instead be engineered to a Tregphenotype Here scFv affinity could be optimizedusing single-site complementarity determining re-gions mutations Treg signaling and activity woulddepend on the CAR signaling domain specificityand affinity as well as density of AQP4 displayedon target cells Animal models that more preciselyrecapitulate human disease would represent signifi-cant advances toward these goals
The TcR approach offers potential advantage overscFvCAR including direct targeting of Treg activityto class Indashbearing tissues and regional lymph nodeswhere AQP4 might be cross-presented by MHCOstensibly this strategy might arrest or reversecross-presentation of AQP4 antigens by class IIMHC which may occur early in the developmentof NMOSDe14 If so this approach may also mitigateepitope spreading The generation of TcRs againstAQP4 peptides that bind to HLA-A2 would be rela-tively straightforward given the extensive knowledgeof its binding motifs and the likelihood that AQP4contains cognate epitopese15 The TcR affinity in thisscenario may prove inadequate but such limitations
might be overcome through the generation of CARmutants
AQP4 antigenndashspecific approaches must addressissues well beyond TcR and CAR affinity Theseinclude untoward conversion of Tregs to pathogenicphenotypes such as those exhibiting either Th1 orTh17 paradigmse16 accurate tissue targeting and pro-motion of memory and stabilitye17e18 It is possiblethat the same tolerance checkpoints currently beingconsidered for rapid deletion of undesirable T cellstransferred in cancer could be applied to NMOSD
SUMMARY AND PROSPECTUS PART I Immunetolerization attempts to restore central or peripheral tol-erance thereby suppressing autoimmune diseases Thereis reason for optimism that this approach might offersubstantial clinical benefit to individuals with NMOSD DNA vaccination T cell vaccination tolerogenicDCs and peptide-coupling strategies have advancedto clinic trials Other promising approaches (egCAR-based or Treg-based therapeutics) are in earlierdevelopmental stages in autoimmune diseases Yetdefining target cell lineages to yield durable efficacyand determining stage of disease development optimalfor intervention remain crucial ongoing challenges Pastfailures using similar strategies have provided criticalinsights that despite the complexities of existing andemerging technologies for promoting tolerance shouldilluminate the pathway forward If so such advances intreatment could benefit individuals with NMOSDand other autoimmune diseases including arrestingdisease progression prolonging intervals to remissionenabling tissue repair and ultimately avoiding chronicimmunosuppressive therapy
AUTHOR CONTRIBUTIONSLarry Steinman draftingrevising the manuscript Amit Bar-Or drafting
revising the manuscript Jacinta M Behne revising the manuscript
Daniel Benitez-Ribas draftingrevising the manuscript Peter S Chin
draftingrevising the manuscript Michael Clare-Salzler draftingrevising
the manuscript Donald Healey draftingrevising the manuscript James
I Kim draftingrevising the manuscript David M Kranz draftingrevis-
ing the manuscript Andreas Lutterotti draftingrevising the manuscript
Roland Martin draftingrevising the manuscript Sven Schippling draft-
ingrevising the manuscript Pablo Villoslada draftingrevising the man-
uscript Cheng-Hong Wei draftingrevising the manuscript Howard L
Weiner draftingrevising the manuscript Scott S Zamvil draftingrevis-
ing the manuscript Michael R Yeaman draftingrevising the manu-
script study concept or design analysis or interpretation of data
accepts responsibility for conduct of research and will give final approval
Terry J Smith draftingrevising the manuscript study concept or design
analysis or interpretation of data accepts responsibility for conduct of
research and will give final approval
ACKNOWLEDGMENTWe thank Dr Gerald Nepom Dr Philip Bernstein and Dr William St
Clair for their helpful comments
STUDY FUNDINGThis work was supported in part by the Guthy-Jackson Charitable
Foundation
Neurology Neuroimmunology amp Neuroinflammation 7
ordf 2016 American Academy of Neurology Unauthorized reproduction of this article is prohibited
DISCLOSUREL Steinman served on the scientific advisory board for Novartis Recep-
tos Atreca Tolerion Teva received travel funding andor speaker hon-
oraria from Biogen Bayhill Bayer Celgene Receptos is on the editorial
board for Multiple Sclerosis Journal Proceedings of the National Academy of
Science holds a patents for antigen-specific tolerance has a patent pend-
ing for cytokines and type 1 interferons is on the speakers bureau for
EMD Serono received research support from NIH has stock options
and board membership in Tolerion is on the board of directors for
BioAtla A Bar-Or is on the scientific advisory board for Dionix
Receptos-Celgene RocheGenentech Novartis GSK Guthy-Jackson
Greater Good Foundation Immune Tolerance Network received travel
funding andor speaker honoraria from Receptos-Celgene RocheGen-
entech Novartis Sanofi-Genzyme GSK served on the editorial board
for Neurologyreg Clinical and Experimental Neuroimmunology consulted
for DioGenix Receptos-Celgene RocheGenentech Novartis Sanofi-
Genzyme GSK received research support from Novartis Sanofi-
Genzyme JM Behne and D Benitez-Ribas report no disclosures PS
Chin served as the medical director for Genentech and Novartis Pharma-
ceuticals Corp M Clare-Salzler received research support from NIH
NIAID D Healy has a patent pending for the treatment of B cellndash
mediated autoimmunities with T cell vaccination has been CSO for
Opexa Therapeutics received research support from and holds stock
stock options in Opexa Therapeutics JI Kim is an associate editor for
the Journal of Negative Results in Biomedicine is employed by Unum
Therapeutics DM Kranz has patents and patents pending in the areas
of yeast display and T cell receptor engineering and receives license fee
payments from them consulted for AbbVie received research support
from NIH Melanoma Research Alliance A Lutterotti served on the
scientific advisory board for Bayer Biogen Novartis Genzyme received
travel support from European Charcot Foundation Fundacio GAEM
holds a patent with the University of Zurich received research support
from Wyss Translational Center Austrian MS Society R Martin served
on the scientific advisory board for Biogen Merck Serono Teva
Genzyme Sanofi-Aventis CellProtect Neuway received speaker hono-
raria from Biogen Merck Serono Novartis Roche Genzyme holds
a patent for the therapeutic efficacy of anto-CD25 monoclonal antibody
treatment in combination with IFN-b in MS consulted for Myelin
Repair Foundation The Weatherall Institute of Molecular Studies Uni-
versity of Oxford the Hertie Foundation is a member of the Kuratorium
of the Jung Foundation for Science received research support from
Novartis Biogen Swiss National Science Foundation European Union
Seventh Framework Program European Research Council S Schippling
served on the scientific advisory board for Bayer Healthcare Biogen
Merck Serono Novartis Sanofi-Genzyme TEVA received travel fund-
ing andor speaker honoraria from Bayer Healthcare Biogen Merck
Serono Novartis Sanofi-Aventis TEVA is an associate editor for Fron-
tiers in Neurology holds a patent for therapeutic vaccination in PML
using VP1 and Il7 received research support from Sanofi-Genzyme
Novartis University of Zurich Betty and David Koetser Foundation
for Brain Research Swiss Multiple Sclerosis Society P Villoslada received
travel funding andor speaker honoraria from Novartis Roche Genzyme
served as an academic editor for PLoS One served on the editorial board
for Neurology and Therapy Current Treatment Options in Neurology Mul-
tiple Sclerosis and Demyelinating Disorders holds a patent for methylthioa-
denosine for the treatment of MS Agnostic neurotrophic compounds for
the treatment of brain diseases Gene signature pattern as a biomarker for
MS Algorithm for quantifying fractal dimension in brain MRI received
research support from Novartis Roche Genzyme Instituto de Salud
Carlos III European Commission National MS Society Fundacion
Maraton TV3 holds stock or stock options in Bionure Inc Spire Bio-
ventures Mint-Labs C-H Wei reports no disclosures HL Weiner
served on the scientific advisory board for the Guthy-Jackson Charitable
Foundation Teva Pharmaceutical Industries Biogen Idec Novartis
Sanofi-Aventis consulted for Therapix Biogen Novartis Serono Teva
Sanofi received research support from National Multiple Sclerosis Soci-
ety S Zamvil served on the scientific advisory board for BioMS Teva
Pharmaceuticals Eli Lilly and Co Myelin Repair Foundation is deputy
editor for Neurologyreg Neuroimmunology amp Neuroinflammation consulted
for Biogen Idec Teva Neuroscience EMD Serono Genzyme Novartis
Roche is on the speakers bureau for Advanced Health Media Biogen
received research support from NIH NMSS Alexander M and June L
Maisin Foundation MR Yeaman is on the scientific advisory board for
Guthy-Jackson Charitable Foundation served as an associate editor for
PLoS Pathogens holds patents for Vaccines targeting drug-resistant patho-
gens immunotherapies targeting drug-resistant pathogens novel anti-
infective biological therapeutics novel anti-infective small molecules
novel biological regulating programmed cell death consulted for
Guthy-Jackson Charitable Foundation received research support from
NovaDigm Therapeutics Inc Metacin Inc US Department of Defense
NIH holds stock or stock options for NovaDigm Therapeutics Inc Meta-
cin Inc receives license fee and royalty payments from NovaDigm Thera-
peutics TJ Smith received research support from NIH University of South
Denmark Bell Charitable Foundation RPB Foundation is a member of the
Guthy-Jackson Charitable Foundation scientific advisory board and holds
patents covering the blockade of insulin-like growth factor receptor-l in
autoimmune diseases Go to Neurologyorgnn for full disclosure forms
Received February 12 2016 Accepted in final form July 15 2016
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10 Turley DM Miller SD Prospects for antigen-specific tol-
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of carbonic anhydrase I ameliorates murine experimental
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12 Genain CP Abel K Belmar N et al Late complications of
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13 Dolgin E The inverse of immunity Nat Med 201016
740ndash743
14 Steinman L Inverse vaccination the opposite of Jennerrsquos
concept for therapy of autoimmunity J Intern Med 2010
267441ndash451
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15 Hinson SR McKeon A Lennon VA Neurological auto-
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19 Feldmann M Steinman L Design of effective immuno-
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20 Garren H Robinson WH Krasulovaacute E et al Phase 2 trial
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21 Bar-Or A Vollmer T Antel J et al Induction of antigen-
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1407ndash1415
22 Selmi C Autoimmunity in 2012 Clin Rev Allergy
Immunol 201345290ndash301
23 Martin A Tisch RM Getts DR Manipulating T cell-
mediated pathology targets and functions of monoclonal
antibody immunotherapy Clin Immunol 2013148
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24 Salvana EM Salata RA Infectious complications associ-
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25 Madakamutil LT Maricic I Sercarz EE Kumar V
Immunodominance in the TCR repertoire of a TCR
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26 Buckner JH Mechanisms of impaired regulation by CD4
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27 Linhares UC Schiavoni PB Barros PO et al The ex vivo
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28 Pohl M Fisher MT Mader S et al Pathogenic T cells
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29 Varrin-Doyer M Spencer CM Schulze-Topphoff U et al
Aquaporin 4-specific T cells in neuromyelitis optica
exhibit a Th17 bias and recognize clostridium ABC trans-
porter Ann Neurol 20127253ndash64
30 Kumar V Sercarz EE The involvement of T cell receptor
peptide-specific regulatory CD41 T cells in recovery from
antigen-induced autoimmune disease J Exp Med 1993
178909ndash916
31 Zhang J T cell vaccination as an immunotherapy for
autoimmune diseases Cell Mol Immunol 20041321ndash
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32 Huang X Wu H Lu Q The mechanisms and applica-
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prehensive review Clin Rev Allergy Immunol 201447
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33 Fontana R Bregni M Cipponi A et al Peripheral blood
lymphocytes genetically modified to express the selftumor
antigen MAGE-A3 induce antitumor immune responses
in cancer patients Blood 20091131651ndash1660
34 Fox E Wynn D Cohan S Rill D McGuire D
Markowitz C A randomized clinical trial of autolo-
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ysis and implications for trial design Mult Scler 2012
18843ndash852
35 Chen G Li N Zang YC et al Vaccination with selected
synovial T cells in rheumatoid arthritis Arthritis Rheum
200756453ndash463
36 Li ZG Mu R Dai ZP Gao XM T cell vaccination in
systemic lupus erythematosus with autologous activated T
cells Lupus 200514884ndash889
37 Leavy O Dendritic cells tracing the origins of cDCs Nat
Rev Immunol 201313703
38 Banchereau J Palucka AK Dendritic cells as therapeutic
vaccines against cancer Nat Rev Immunol 20055296ndash
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39 Vacchelli E Vitale I Eggermont A et al Trial watch
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Oncoimmunology 201211111ndash1134
40 Mantia-Smaldone GM Chu CS A review of dendritic cell
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41 Segovia-Gamboa N Rodriguez-Arellano ME Rangel-
Cruz R et al Tolerogenic dendritic cells induce antigen-
specific hyporesponsiveness in insulin- and glutamic acid
decarboxylase 65-autoreactive T lymphocytes from type 1
diabetic patients Clin Immunol 201415472ndash83
42 Giannoukakis N Phillips B Finegold D Harnaha J
Trucco M Phase I (safety) study of autologous tolerogenic
dendritic cells in type 1 diabetic patients Diabetes Care
2011342026ndash2032
43 Boks MA Kager-Groenland J Haasjes MSP Zwaginga JJ
van Ham SM ten Brinke A IL-10-generated tolerogenic
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induction- a comparative study on human clinical-
applicable DC Clin Immunol 2012142332ndash342
44 Saito M Nagasaw M Takada H et al Defective IL-10
signaling in hyper-IgE syndrome results in impaired gen-
eration of tolerogenic dendritic cells and induced regula-
tory T cells J Exp Med 2011208235ndash249
45 Miller SD Turley DM Podojil JR Antigen-specific tol-
erance strategies for the prevention and treatment of auto-
immune disease Nat Rev Immunol 20077665ndash677
46 Karpus WJ Pope JG Peterson JD Dal Canto MC
Miller SD Inhibition of Theilerrsquos virus-mediated demye-
lination by peripheral immune tolerance induction
J Immunol 1995155947ndash957
47 Kennedy KJ Smith WS Miller SD Karpus WJ Induc-
tion of antigen-specific tolerance for the treatment of
ongoing relapsing autoimmune encephalomyelitis a com-
parison between oral and peripheral tolerance J Immunol
19971591036ndash1044
48 Kennedy MK Tan LJ Dal Canto MC et al Inhibition of
murine relapsing experimental autoimmune encephalomy-
elitis by immune tolerance to proteolipid protein and its
encephalitogenic peptides J Immunol 1990144909ndash
915
49 Turley DM Miller SD Peripheral tolerance induction
using ethylenecarbodiimide-fixed APCs uses both direct
and indirect mechanisms of antigen presentation for
Neurology Neuroimmunology amp Neuroinflammation 9
ordf 2016 American Academy of Neurology Unauthorized reproduction of this article is prohibited
prevention of experimental autoimmune encephalomyeli-
tis J Immunol 20071782212ndash2220
50 Getts DR Turley DM Smith CE et al Tolerance
induced by apoptotic antigen-coupled leukocytes is
induced by PD-L11 and IL-10-producing splenic macro-
phages and maintained by T regulatory cells J Immunol
20111872405ndash2417
51 Luo X Pothoven KL McCarthy D et al ECDI-fixed allo-
geneic splenocytes induce donor-specific tolerance for long-
term survival of islet transplants via two distinct mechanisms
Proc Natl Acad Sci USA 200810514527ndash14532
52 Lutterotti A Yousef S Sputtek A et al Antigen-specific
tolerance by autologous myelin peptide-coupled cells
a phase 1 trial in multiple sclerosis Sci Transl Med
20135188ra75
53 Bielekova B SungMH KadomN Simon R McFarland HF
Martin R Expansion and functional relevance of high-avidity
myelin-specific CD41 T cells in multiple sclerosis
J Immunol 20041723893ndash3904
54 Jenkins MK Mueller D Schwartz RH et al Induction
and maintenance of anergy in mature T cells Adv Exp
Med Biol 1991292167ndash176
55 Peschl P Reindl M Schanda K Sospedra M
Martin R Lutterotti A Anti-myelin antibody re-
sponses following induction of antigen-specific toler-
ance with antigen-coupled cells J Neuroimmunol
201427512
56 Tuohy VK Yu M Yin L Kawczak JA Kinkel PR Regres-
sion and spreading of self-recognition during the develop-
ment of autoimmune demyelinating disease J Autoimmun
19991311ndash20
57 Vanderlugt CL Miller SD Epitope spreading in immune-
mediated diseases implications for immunotherapy Nat
Rev Immunol 2002285ndash95
58 Stromnes IM Schmitt TM Chapuis AG Hingorani SR
Greenberg PD Re-adapting T cells for cancer therapy
from mouse models to clinical trials Immunol Rev
2014257145ndash164
10 Neurology Neuroimmunology amp Neuroinflammation
ordf 2016 American Academy of Neurology Unauthorized reproduction of this article is prohibited
DOI 101212NXI000000000000027620163 Neurol Neuroimmunol Neuroinflamm
Larry Steinman Amit Bar-Or Jacinta M Behne et al Restoring immune tolerance in neuromyelitis optica Part I
This information is current as of September 7 2016
2016 American Academy of Neurology All rights reserved Online ISSN 2332-7812Published since April 2014 it is an open-access online-only continuous publication journal Copyright copy
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is an official journal of the American Academy of NeurologyNeurol Neuroimmunol Neuroinflamm
self-antigens in an absence of adequate regulatorycounterbalance autoimmune disease might result26
In NMOSD the pathogenic antibody response isdominated by class-switched IgG indicating anunderlying AQP4-reactive T cell activity2728 Inaddition Th17 polarization of naive T cells leads tothe secretion of proinflammatory cytokines in relationto target lesions including interleukin (IL)-17A andgranulocyte macrophagendashcolony stimulating factor29
Such a proinflammatory cytokine profile maypromote tissue destruction through recruitment ofgranulocytes or other cells capable of antibody-dependent cell cytotoxicity
The observation that anti-idiotypic Tregs can arisespontaneously and be associated with the resolution ofautoimmunity led to the concept of T cell vaccina-tion3031 This strategy invokes multiple immune func-tions that may mitigate autoimmune disease Forexample induction of idiotype-restricted CD41CD251FoxP31 Tregs IL-10ndashsecreting CD41 Tr1cells and CD81 cytotoxic T cells can result each capableof selectively targeting autoreactive T cells that expressthe idiotype represented by the immunizing T cells32
T cellndashbased vaccines typically use attenuatedautologous autoreactive effector T cells as immuno-gens32 in an amplification strategy designed to targetand counteract autoreactive T cells Theoreticallythis process should selectively deplete andor func-tionally attenuate pathogenic T cells carrying the cor-responding idiotype Restoring an adequate Treg
influence theoretically should mitigate tissue dysfunc-tion Clinical trials have suggested a therapeutic ben-efit from T cell vaccination with minimal sideeffects33 In this regard proof-of-principle has beendemonstrated in several animal models31 In additiontherapeutic trials applying this strategy to MS rheu-matoid arthritis and systemic lupus erythematosusndashassociated nephritis have been undertaken In some ofthese conditions a T cellndashbased vaccine approach hasdemonstrated considerable promise34ndash36
The manufacture of autologous T cell vaccines isdependent first on identifying autoantigen-specificT cells within the peripheral blood of that patientThis goal is achieved by performing an epitope profil-ing assay using overlapping peptides comprising theamino acid sequence of the target antigen The assayreadout is based on the detection of proinflammatorycytokines induced by immunodominant peptideswithin the target antigen This strategy thereby iden-tifies peptides and the associated T cell responsesmost relevant to the autoimmune disease in anaffected individual Next bulk T cell lines areexpanded in vitro from an autologous peripheralblood sample against the selected peptides to providesufficient numbers of autoreactive T cells to meetdosing requirements These particular T cells are ali-quoted and cryopreserved On demand a dose-equivalent is thawed formulated and attenuatedby irradiation before subcutaneous administrationMethods currently in use allow a single-cycle
Figure 3 Hypothesized cellular and molecular crosstalk influencing NMOSD
(A) Theoretical proinflammatory interactions that arise from loss of immune tolerance which may promote the onset or pro-gression of neuromyelitis opticaspectrum disorder (NMOSD) (B) Theoretical anti-inflammatory interactions that arise andcould be amenable to therapeutic intervention in restoring immune tolerance in NMOSD (Cellular) APC5 antigen-present-ing cell Bc5B cell Tc5 T cell (cell surface determinants) CD5 class designation MHC5major histocompatibility complex(soluble) BAFF 5 B cell activating factor IL 5 interleukin IL-18BP 5 IL-18 binding protein sIL-1R 5 soluble IL-1 receptorsTNFR 5 soluble tumor necrosis factor a receptor
4 Neurology Neuroimmunology amp Neuroinflammation
ordf 2016 American Academy of Neurology Unauthorized reproduction of this article is prohibited
manufacturing process from one blood collection tobe used to generate sufficient drug for at least 1 yearof treatment
Considering the aggregate evidence a compellingcase can be made for the critical role of T cells inthe pathogenesis of NMOSD29 It follows thatreestablishment of immune tolerance to AQP4through vaccination with autologous attenuatedand idiotype-restricted T cells represents a promisingnew approach to immunotherapy in this disease
Dendritic cell vaccines Dendritic cells (DCs) presentantigens to immune cells including those with regula-tory and autoreactive function through major histo-compatibility complex (MHC) class I and IIrestriction Normally DCs present self-antigens butdo not drive autoreactive immune responses Thusautoreactive T cells are inactivated or anergizedbecause of low MHC expression inadequatecostimulation (eg inducing anergy) or tolerogeniccostimulation (eg PD-L1 [programed death ligand1] IL-10 etc) In addition DCs have important rolesin maintaining tolerance by promoting regulatorycells such as CD41 and CD81 Tregs and IL-10ndashproducing regulatory B cells37 Immature human DCsare readily generated in the laboratory from bloodmonocytes and can be engineered to maintaintolerogenic phenotypes Indeed DC-based therapieshave now entered clinical trials focusing on NMOSD(ClinicalTrialsgov identifier NCT02283671)
As a strategy for inducing immune tolerance inT1D DC administration has proven relatively safe38ndash42 Here granulocyte macrophagendashcolony stimulatingfactorIL-4 was used to generate immature CD11c1
DCs from monocytes in vitro Transition to a proin-flammatory DC phenotype is concerning when DCsare administered to human patients To mitigate thisconcern immature DCs can be treated in vitro withimmunosuppressive agents such as corticosteroids vita-min D3 andor IL-1043 before their use as tolerizingvaccines The resulting DCs exhibit durable tolerogenicphenotypes that prolong organ allograft survival inmice Notably this conditioning enhances the tolero-genic properties of DCs44 and induces high-level IL-10production which inhibit activated Th1 and Th17cells induce Tregs and suppress autoreactive B cellfunctions A related strategy involves loading of imma-ture DCs with antisense oligonucleotides targeting keycostimulatory molecules such as CD40 CD80 orCD86 Alternatively viral vectors encoding immuno-regulatory factors may potentiate tolerogenic DCs Ofnote the maintenance of DC immaturity also allowsthese cells to migrate into lymph nodes following anti-gen exposure enhancing their tolerogenic potential
Loading immature DCs with either native or syn-thetic peptides or whole antigens may also promote
antigen-specific tolerance through induction of regula-tory lymphocytes This strategy addresses multiple auto-antigens andor epitopes within a target antigen(s)Thus the goal is conditional presentation of self-antigenic epitopes by DCs to yield Treg inductionUsing the entire antigen affords presentation of a broad-er repertoire of peptides selected by relevant MHC al-leles and potentially avoids artificial epitope(s)selection In this respect preloading microparticlescontaining antigen might facilitate DC uptake andfavor tolerogenic phenotypes Of note early studiesin which rodents were vaccinated with autoantigen-loaded DCs demonstrated antigen-specific durableand effective tolerance These principles have beenapplied in human trials of T1D42 Advantages mayalso emerge from strategies in which DCs are al-lowed to antigen-load in situ
Small phase I clinical trials exploring DC vaccinesin autoimmune disease have largely focused on T1Dor rheumatoid arthritis Evidence to date suggests thattolerizing DC therapies are safe However larger trialswill be required to more thoroughly assess safety andefficacy Despite the great interest in further assessingDC vaccines in the prevention modulation and cur-ing of autoimmune diseases key questions remainThese include optimization of DC dosing vaccina-tion frequency (eg boosting) route of administra-tion and identification or validation of biomarkersfor assessing therapeutic efficacy
NMOSD appears to represent an ideal disease forDC therapy because a dominant and T cellndashrestrictedautoantigen (AQP4) has been identified If successfulthis approach should be used as early in the diseasecourse as is possible The rationale for this strategy isto prevent or ameliorate the expansion of inflamma-tory AQP4-restricted or other antigen-restrictedT lymphocytes thereby minimizing broader tissueinjury In addition such early intervention could inconcept minimize the potential for antigen spreadingor reversal of antigenic ignorance
Antigen-coupled presentation Antigen-specific toleriza-tion in animal models of disease has been pursued bya number of laboratory groups Among these modelsare those for experimental autoimmune encephalo-myelitis (EAE) Theiler virusndashinduced encephalitistolerance to organ transplantation and therapyfor severe allergy645ndash50 Use of peptide-coupled orchemically fixed antigen-presenting cells (APCs) byMiller et al6 has been one strategy Here unmodifiedsplenocytes are pulsed in vitro with a disease-relevantautoantigen(s) together with a coupling agent Thehost is immunized using these cells with the goal ofinducing tolerance to a target autoantigen(s) Thisapproach was effective in reducing disease activityin a model of relapsing-remitting EAE in SJL
Neurology Neuroimmunology amp Neuroinflammation 5
ordf 2016 American Academy of Neurology Unauthorized reproduction of this article is prohibited
mice4547 The strategy may also be attractive asprophylaxis for allotransplantation and the attenuationof alloreactive immune responses51 Key advantages ofthis approach may include (1) extensive experience inEAE and other autoimmune disease models (2) realisticpotential for targeting multiple autoantigens (3)acceptable safety profiles in preclinical studies (4) anapparent absence of epitope spreading and (5) positivepreliminary evidence for efficacy45 These attributesprovide a strong rationale for applying this approach toindividuals with antigenically characterized autoimmunediseases such as NMOSD
This strategy has been translated to human trialsusing unaltered autologous peripheral blood mono-nuclear cells (PBMCs) as APCs following their isola-tion by therapeutic leukapheresis52 In MS isolatedcells are first pulsed in vitro with a mixture of immu-nodominant myelin peptides that are identified astargets of the high-avidity myelin-specific T cellresponse53 A phase I trial was conducted in whichpatients received graded dosages of peptide-coupledand fixed autologous PBMCs52 Results suggested thisapproach was well tolerated and induced neitherimmune reactivity to myelin peptides nor triggereddisease relapses Rather the data demonstrated thatthe abundance of myelin-reactive T cells was substan-tially reduced52 Several putative mechanisms ofaction have been attributed to this strategy FirstAPC presentation of cross-linked peptide(s) can leadto anergy andor functional silencing of T cells result-ing from inadequate second-signaling4954 Here thedominant mechanisms are most likely related to Tcell recognition of peptide-coupled antigen com-plexes In this scenario T cells andor APCs undergoapoptosis and are phagocytosed by a special subset ofimmature DCs or macrophages in the splenic mar-ginal zone454950 These immature DCsmacrophagescan present an autoantigen peptide(s) in a tolerogenicmanner (eg IL-10) to pathogenic autoreactiveT cells Each of these pathways might help dampenautoimmunity Induction of Tregs is also likely criticalin suppressing autoreactivity45 Tolerization withpeptide-coupled fixed PBMCs undergoing apoptosisin vivo does not lead to the induction of anti-DNAautoantibodies55 The relevant mechanism(s) involvedin this immune tolerizing strategy and how it might beapplied to human diseases require further study
Despite promising results in animals humanstudies have been limited to demonstrating that theabundance of myelin-specific T cells is substantiallyreduced52 however this phase I study was not designedto elucidate specific mechanisms of action The abovetolerization approach with AQP4-coupled fixed APCscould in theory be readily adapted to NMOSDMoreover epitope spreading can occur in this disease56
and may be attenuated by this strategy (reviewed in
reference 57) Thus tolerization with the entireAQP4 holoprotein or derivative immunodominantpeptides may dampen autoreactivity against AQP4and secondary autoantigen targets providing clinicalbenefit Naturally technical challenges are likely to arisein these therapeutic strategies and their approach to theclinic Moreover specific biomarkers for determiningefficacy will be needed to unambiguously inform thetherapeutic benefit Nonetheless this approach holdspromise for restoring immune tolerance in NMOSDand other autoimmune conditions
T cell receptor engineering The ab TcR is the criticalantigen recognition determinant on CD41 and CD81
T cells conferring both antigen specificity and sensitiv-ity TcR diversity results from rearrangement of multi-ple genes (Figure 2) A key difference from antibodies isthe requirement that ab TcRs bind to peptide ligandsdisplayed through the APC MHC This MHC restric-tion focuses T cell activity against cells that bear therespective antigen This mechanism facilitates targetingto antigen-enriched anatomical sites such as lymphnodes and infected tissues while effector T cellspromote immune responses against foreign orendogenous autoantigens CD41 Tregs suppress theseresponses (part II)
Improved understanding of TcR-driven immunerecognition should yield strategies to harness thesecells in treating autoimmune diseases For exampleautoreactive TcR functions may be redirected tomodulate their disease-propagating phenotypes Bycomparison ongoing efforts to generate therapeuticautologous T cells targeting cancer involve engineer-ing TcRs specific to cancer peptideclass I MHC anti-gens58e1 These cells mature into effector T cells exvivo that are then administered to the patient Initialattempts involved CD81 T cell clones exhibiting rel-atively low affinities (KD range 10ndash100 mM)e1 How-ever subsequent engineering has yielded higheraffinity TcRs with KD ranges from 01 to 1 mMMoreover TcRs targeting class I antigens do notrequire the CD8 coreceptor and are thus capable ofrecruiting effector CD41 T cells against cancer cellsexpressing their cognate target antigene2 Conceiv-ably this approach might also prove useful in target-ing autoreactive cells To date TcR engineering hasbeen accomplished using several methodologiesincluding yeast and phage displaye3
Engineered TcRs can be delivered using a varietyof strategies These include administration as solubleagents via gene transfer or as adoptive T cell thera-pies Soluble TcRs will likely require affinities compa-rable to those of antibodies (KD in nM or pM range)and formatting as immunoglobulin fusions bispecificagents with anti-CD3e4 or as fusions with immuno-regulatory molecules such as cytokinese5 A high-
6 Neurology Neuroimmunology amp Neuroinflammation
ordf 2016 American Academy of Neurology Unauthorized reproduction of this article is prohibited
affinity TcR against a class II MHC fused to IL-10has been developed as a means for downregulatingautoimmune responsese6 Optimizing the tissue tar-geting of these constructs remains challenging
Adoptive T cell transfer of enriched Tregs has alsobeen used as a method of antigen-specific toleriza-tione7e8 Yet optimal delivery of antigen-specific sta-ble Tregs remains to be determined Moreover TcRaffinity and specificity of Tregse9 is less well charac-terized than those of effector T cells Several studieshave demonstrated that introducing class IndashrestrictedTcR into Tregs can redirect their specific activitiese10
and that TcRs with KDs of approximately 1 mM maybe of sufficient avidity to drive Treg activitye11 TcRsdirected against class I targets required lower affinitiesthan those in effector CD41 T cells that promoteTreg activity
Opportunities to generate mouse models and ther-apeutic T cells targeting AQP4 would appear to benumerouse12 For example in a mouse model animmunogenic response can be elicited with effectorT cells (CD41 andor CD81) expressing an AQP4-specific antibody (scFv)e13 fused to a chimeric antigenreceptor (CAR) Redirection of Tregs to suppresseffector cells could provide greater therapeutic poten-tial and utilize CARs directed against AQP4 Alter-natively conventional TcRs targeting AQP4 peptideMHC class I complexes such as human leukocyteantigen (HLA)-A2 (which are expressed by 40 ofthe population) might prove effective Possible advan-tages of CARs include their potential use of scFv fromexisting monoclonal antibodiese13 Furthermore thesame scFv used in effector T cells to initiate patho-genic responses could instead be engineered to a Tregphenotype Here scFv affinity could be optimizedusing single-site complementarity determining re-gions mutations Treg signaling and activity woulddepend on the CAR signaling domain specificityand affinity as well as density of AQP4 displayedon target cells Animal models that more preciselyrecapitulate human disease would represent signifi-cant advances toward these goals
The TcR approach offers potential advantage overscFvCAR including direct targeting of Treg activityto class Indashbearing tissues and regional lymph nodeswhere AQP4 might be cross-presented by MHCOstensibly this strategy might arrest or reversecross-presentation of AQP4 antigens by class IIMHC which may occur early in the developmentof NMOSDe14 If so this approach may also mitigateepitope spreading The generation of TcRs againstAQP4 peptides that bind to HLA-A2 would be rela-tively straightforward given the extensive knowledgeof its binding motifs and the likelihood that AQP4contains cognate epitopese15 The TcR affinity in thisscenario may prove inadequate but such limitations
might be overcome through the generation of CARmutants
AQP4 antigenndashspecific approaches must addressissues well beyond TcR and CAR affinity Theseinclude untoward conversion of Tregs to pathogenicphenotypes such as those exhibiting either Th1 orTh17 paradigmse16 accurate tissue targeting and pro-motion of memory and stabilitye17e18 It is possiblethat the same tolerance checkpoints currently beingconsidered for rapid deletion of undesirable T cellstransferred in cancer could be applied to NMOSD
SUMMARY AND PROSPECTUS PART I Immunetolerization attempts to restore central or peripheral tol-erance thereby suppressing autoimmune diseases Thereis reason for optimism that this approach might offersubstantial clinical benefit to individuals with NMOSD DNA vaccination T cell vaccination tolerogenicDCs and peptide-coupling strategies have advancedto clinic trials Other promising approaches (egCAR-based or Treg-based therapeutics) are in earlierdevelopmental stages in autoimmune diseases Yetdefining target cell lineages to yield durable efficacyand determining stage of disease development optimalfor intervention remain crucial ongoing challenges Pastfailures using similar strategies have provided criticalinsights that despite the complexities of existing andemerging technologies for promoting tolerance shouldilluminate the pathway forward If so such advances intreatment could benefit individuals with NMOSDand other autoimmune diseases including arrestingdisease progression prolonging intervals to remissionenabling tissue repair and ultimately avoiding chronicimmunosuppressive therapy
AUTHOR CONTRIBUTIONSLarry Steinman draftingrevising the manuscript Amit Bar-Or drafting
revising the manuscript Jacinta M Behne revising the manuscript
Daniel Benitez-Ribas draftingrevising the manuscript Peter S Chin
draftingrevising the manuscript Michael Clare-Salzler draftingrevising
the manuscript Donald Healey draftingrevising the manuscript James
I Kim draftingrevising the manuscript David M Kranz draftingrevis-
ing the manuscript Andreas Lutterotti draftingrevising the manuscript
Roland Martin draftingrevising the manuscript Sven Schippling draft-
ingrevising the manuscript Pablo Villoslada draftingrevising the man-
uscript Cheng-Hong Wei draftingrevising the manuscript Howard L
Weiner draftingrevising the manuscript Scott S Zamvil draftingrevis-
ing the manuscript Michael R Yeaman draftingrevising the manu-
script study concept or design analysis or interpretation of data
accepts responsibility for conduct of research and will give final approval
Terry J Smith draftingrevising the manuscript study concept or design
analysis or interpretation of data accepts responsibility for conduct of
research and will give final approval
ACKNOWLEDGMENTWe thank Dr Gerald Nepom Dr Philip Bernstein and Dr William St
Clair for their helpful comments
STUDY FUNDINGThis work was supported in part by the Guthy-Jackson Charitable
Foundation
Neurology Neuroimmunology amp Neuroinflammation 7
ordf 2016 American Academy of Neurology Unauthorized reproduction of this article is prohibited
DISCLOSUREL Steinman served on the scientific advisory board for Novartis Recep-
tos Atreca Tolerion Teva received travel funding andor speaker hon-
oraria from Biogen Bayhill Bayer Celgene Receptos is on the editorial
board for Multiple Sclerosis Journal Proceedings of the National Academy of
Science holds a patents for antigen-specific tolerance has a patent pend-
ing for cytokines and type 1 interferons is on the speakers bureau for
EMD Serono received research support from NIH has stock options
and board membership in Tolerion is on the board of directors for
BioAtla A Bar-Or is on the scientific advisory board for Dionix
Receptos-Celgene RocheGenentech Novartis GSK Guthy-Jackson
Greater Good Foundation Immune Tolerance Network received travel
funding andor speaker honoraria from Receptos-Celgene RocheGen-
entech Novartis Sanofi-Genzyme GSK served on the editorial board
for Neurologyreg Clinical and Experimental Neuroimmunology consulted
for DioGenix Receptos-Celgene RocheGenentech Novartis Sanofi-
Genzyme GSK received research support from Novartis Sanofi-
Genzyme JM Behne and D Benitez-Ribas report no disclosures PS
Chin served as the medical director for Genentech and Novartis Pharma-
ceuticals Corp M Clare-Salzler received research support from NIH
NIAID D Healy has a patent pending for the treatment of B cellndash
mediated autoimmunities with T cell vaccination has been CSO for
Opexa Therapeutics received research support from and holds stock
stock options in Opexa Therapeutics JI Kim is an associate editor for
the Journal of Negative Results in Biomedicine is employed by Unum
Therapeutics DM Kranz has patents and patents pending in the areas
of yeast display and T cell receptor engineering and receives license fee
payments from them consulted for AbbVie received research support
from NIH Melanoma Research Alliance A Lutterotti served on the
scientific advisory board for Bayer Biogen Novartis Genzyme received
travel support from European Charcot Foundation Fundacio GAEM
holds a patent with the University of Zurich received research support
from Wyss Translational Center Austrian MS Society R Martin served
on the scientific advisory board for Biogen Merck Serono Teva
Genzyme Sanofi-Aventis CellProtect Neuway received speaker hono-
raria from Biogen Merck Serono Novartis Roche Genzyme holds
a patent for the therapeutic efficacy of anto-CD25 monoclonal antibody
treatment in combination with IFN-b in MS consulted for Myelin
Repair Foundation The Weatherall Institute of Molecular Studies Uni-
versity of Oxford the Hertie Foundation is a member of the Kuratorium
of the Jung Foundation for Science received research support from
Novartis Biogen Swiss National Science Foundation European Union
Seventh Framework Program European Research Council S Schippling
served on the scientific advisory board for Bayer Healthcare Biogen
Merck Serono Novartis Sanofi-Genzyme TEVA received travel fund-
ing andor speaker honoraria from Bayer Healthcare Biogen Merck
Serono Novartis Sanofi-Aventis TEVA is an associate editor for Fron-
tiers in Neurology holds a patent for therapeutic vaccination in PML
using VP1 and Il7 received research support from Sanofi-Genzyme
Novartis University of Zurich Betty and David Koetser Foundation
for Brain Research Swiss Multiple Sclerosis Society P Villoslada received
travel funding andor speaker honoraria from Novartis Roche Genzyme
served as an academic editor for PLoS One served on the editorial board
for Neurology and Therapy Current Treatment Options in Neurology Mul-
tiple Sclerosis and Demyelinating Disorders holds a patent for methylthioa-
denosine for the treatment of MS Agnostic neurotrophic compounds for
the treatment of brain diseases Gene signature pattern as a biomarker for
MS Algorithm for quantifying fractal dimension in brain MRI received
research support from Novartis Roche Genzyme Instituto de Salud
Carlos III European Commission National MS Society Fundacion
Maraton TV3 holds stock or stock options in Bionure Inc Spire Bio-
ventures Mint-Labs C-H Wei reports no disclosures HL Weiner
served on the scientific advisory board for the Guthy-Jackson Charitable
Foundation Teva Pharmaceutical Industries Biogen Idec Novartis
Sanofi-Aventis consulted for Therapix Biogen Novartis Serono Teva
Sanofi received research support from National Multiple Sclerosis Soci-
ety S Zamvil served on the scientific advisory board for BioMS Teva
Pharmaceuticals Eli Lilly and Co Myelin Repair Foundation is deputy
editor for Neurologyreg Neuroimmunology amp Neuroinflammation consulted
for Biogen Idec Teva Neuroscience EMD Serono Genzyme Novartis
Roche is on the speakers bureau for Advanced Health Media Biogen
received research support from NIH NMSS Alexander M and June L
Maisin Foundation MR Yeaman is on the scientific advisory board for
Guthy-Jackson Charitable Foundation served as an associate editor for
PLoS Pathogens holds patents for Vaccines targeting drug-resistant patho-
gens immunotherapies targeting drug-resistant pathogens novel anti-
infective biological therapeutics novel anti-infective small molecules
novel biological regulating programmed cell death consulted for
Guthy-Jackson Charitable Foundation received research support from
NovaDigm Therapeutics Inc Metacin Inc US Department of Defense
NIH holds stock or stock options for NovaDigm Therapeutics Inc Meta-
cin Inc receives license fee and royalty payments from NovaDigm Thera-
peutics TJ Smith received research support from NIH University of South
Denmark Bell Charitable Foundation RPB Foundation is a member of the
Guthy-Jackson Charitable Foundation scientific advisory board and holds
patents covering the blockade of insulin-like growth factor receptor-l in
autoimmune diseases Go to Neurologyorgnn for full disclosure forms
Received February 12 2016 Accepted in final form July 15 2016
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of neuromyelitis optica Adv Immunol 2014121213ndash242
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3 Lennon VA Kryzer TJ Pittock SJ Verkman AS
Hinson SR IgG marker of optic-spinal multiple sclerosis
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ability and retention rate of azathioprine in 103 aquaporin-4
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5 Nakamura M Nakazawa T Doi H et al Early high-dose
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myelitis optica Graefes Arch Clin Exp Ophthalmol 2010
2481777ndash1785
6 Miller SD Wetzig RP Claman HN The induction of
cell-mediated immunity and tolerance with protein anti-
gens coupled to syngeneic lymphoid cells J Exp Med
1979149758ndash773
7 Oh S Schwartz RH Singh NJ Development and toler-
ization of hyperacute rejection in a transgenic mouse graft
versus host model Transplantation 201294234ndash240
8 Castells M Rapid desensitization of hypersensitivity reactions
to chemotherapy agents Curr Drug Saf 20061243ndash251
9 Fousteri G Bresson D von Herrath M Rational develop-
ment of antigen-specific therapies for type 1 diabetes Adv
Exp Med Biol 2007601313ndash319
10 Turley DM Miller SD Prospects for antigen-specific tol-
erance based therapies for the treatment of multiple scle-
rosis Results Probl Cell Differ 201051217ndash235
11 Mori K Yamanishi H Ikeda Y et al Oral administration
of carbonic anhydrase I ameliorates murine experimental
colitis induced by Foxp3-CD41CD252 T cells J Leukoc
Biol 201393963ndash972
12 Genain CP Abel K Belmar N et al Late complications of
immune deviation therapy in a nonhuman primate Sci-
ence 19962742054ndash2057
13 Dolgin E The inverse of immunity Nat Med 201016
740ndash743
14 Steinman L Inverse vaccination the opposite of Jennerrsquos
concept for therapy of autoimmunity J Intern Med 2010
267441ndash451
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ordf 2016 American Academy of Neurology Unauthorized reproduction of this article is prohibited
15 Hinson SR McKeon A Lennon VA Neurological auto-
immunity targeting aquaporin-4 Neuroscience 2010168
1009ndash1018
16 Sato DK Callegaro D de Haidar Jorge FM et al Cere-
brospinal fluid aquaporin-4 antibody levels in neuromye-
litis optica attacks Ann Neurol 201476305ndash309
17 Steinman L The re-emergence of antigen-specific toler-
ance as a potential therapy for MS Mult Scler 201521
1223ndash1238
18 Roep BO Solvason N Gottlieb PA et al Plasmid
encoded proinsulin preserves C-peptide while specifically
reducing proinsulin specific CD8 T cells in type 1 diabe-
tes Sci Transl Med 20135191ra82
19 Feldmann M Steinman L Design of effective immuno-
therapy for human autoimmunity Nature 2005435
612ndash619
20 Garren H Robinson WH Krasulovaacute E et al Phase 2 trial
of a DNA vaccine encoding myelin basic protein for mul-
tiple sclerosis Ann Neurol 200863611ndash620
21 Bar-Or A Vollmer T Antel J et al Induction of antigen-
specific tolerance in multiple sclerosis after immunization
with DNA encoding myelin basic protein in a randomized
placebo-controlled phase 12 trial Arch Neurol 200764
1407ndash1415
22 Selmi C Autoimmunity in 2012 Clin Rev Allergy
Immunol 201345290ndash301
23 Martin A Tisch RM Getts DR Manipulating T cell-
mediated pathology targets and functions of monoclonal
antibody immunotherapy Clin Immunol 2013148
136ndash147
24 Salvana EM Salata RA Infectious complications associ-
ated with monoclonal antibodies and related small mole-
cules Clin Microbiol Rev 200922274ndash290
25 Madakamutil LT Maricic I Sercarz EE Kumar V
Immunodominance in the TCR repertoire of a TCR
peptide-specific CD41 Treg population that controls
experimental autoimmune encephalomyelitis J Immunol
20081804577ndash4585
26 Buckner JH Mechanisms of impaired regulation by CD4
(1)CD25(1)FOXP3(1) regulatory T cells in human
autoimmune diseases Nat Rev Immunol 201010849ndash
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27 Linhares UC Schiavoni PB Barros PO et al The ex vivo
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28 Pohl M Fisher MT Mader S et al Pathogenic T cells
responses against aquaporin 4 Acta Neuropathol 2011
12221ndash34
29 Varrin-Doyer M Spencer CM Schulze-Topphoff U et al
Aquaporin 4-specific T cells in neuromyelitis optica
exhibit a Th17 bias and recognize clostridium ABC trans-
porter Ann Neurol 20127253ndash64
30 Kumar V Sercarz EE The involvement of T cell receptor
peptide-specific regulatory CD41 T cells in recovery from
antigen-induced autoimmune disease J Exp Med 1993
178909ndash916
31 Zhang J T cell vaccination as an immunotherapy for
autoimmune diseases Cell Mol Immunol 20041321ndash
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32 Huang X Wu H Lu Q The mechanisms and applica-
tions of T cell vaccination for autoimmune diseases a com-
prehensive review Clin Rev Allergy Immunol 201447
219ndash233
33 Fontana R Bregni M Cipponi A et al Peripheral blood
lymphocytes genetically modified to express the selftumor
antigen MAGE-A3 induce antitumor immune responses
in cancer patients Blood 20091131651ndash1660
34 Fox E Wynn D Cohan S Rill D McGuire D
Markowitz C A randomized clinical trial of autolo-
gous T-cell therapy in multiple sclerosis subset anal-
ysis and implications for trial design Mult Scler 2012
18843ndash852
35 Chen G Li N Zang YC et al Vaccination with selected
synovial T cells in rheumatoid arthritis Arthritis Rheum
200756453ndash463
36 Li ZG Mu R Dai ZP Gao XM T cell vaccination in
systemic lupus erythematosus with autologous activated T
cells Lupus 200514884ndash889
37 Leavy O Dendritic cells tracing the origins of cDCs Nat
Rev Immunol 201313703
38 Banchereau J Palucka AK Dendritic cells as therapeutic
vaccines against cancer Nat Rev Immunol 20055296ndash
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39 Vacchelli E Vitale I Eggermont A et al Trial watch
dendritic cell-based interventions for cancer therapy
Oncoimmunology 201211111ndash1134
40 Mantia-Smaldone GM Chu CS A review of dendritic cell
therapy for cancer progress and challenges BioDrugs
201327453ndash468
41 Segovia-Gamboa N Rodriguez-Arellano ME Rangel-
Cruz R et al Tolerogenic dendritic cells induce antigen-
specific hyporesponsiveness in insulin- and glutamic acid
decarboxylase 65-autoreactive T lymphocytes from type 1
diabetic patients Clin Immunol 201415472ndash83
42 Giannoukakis N Phillips B Finegold D Harnaha J
Trucco M Phase I (safety) study of autologous tolerogenic
dendritic cells in type 1 diabetic patients Diabetes Care
2011342026ndash2032
43 Boks MA Kager-Groenland J Haasjes MSP Zwaginga JJ
van Ham SM ten Brinke A IL-10-generated tolerogenic
dendritic cells are optimal for functional regulatory T cell
induction- a comparative study on human clinical-
applicable DC Clin Immunol 2012142332ndash342
44 Saito M Nagasaw M Takada H et al Defective IL-10
signaling in hyper-IgE syndrome results in impaired gen-
eration of tolerogenic dendritic cells and induced regula-
tory T cells J Exp Med 2011208235ndash249
45 Miller SD Turley DM Podojil JR Antigen-specific tol-
erance strategies for the prevention and treatment of auto-
immune disease Nat Rev Immunol 20077665ndash677
46 Karpus WJ Pope JG Peterson JD Dal Canto MC
Miller SD Inhibition of Theilerrsquos virus-mediated demye-
lination by peripheral immune tolerance induction
J Immunol 1995155947ndash957
47 Kennedy KJ Smith WS Miller SD Karpus WJ Induc-
tion of antigen-specific tolerance for the treatment of
ongoing relapsing autoimmune encephalomyelitis a com-
parison between oral and peripheral tolerance J Immunol
19971591036ndash1044
48 Kennedy MK Tan LJ Dal Canto MC et al Inhibition of
murine relapsing experimental autoimmune encephalomy-
elitis by immune tolerance to proteolipid protein and its
encephalitogenic peptides J Immunol 1990144909ndash
915
49 Turley DM Miller SD Peripheral tolerance induction
using ethylenecarbodiimide-fixed APCs uses both direct
and indirect mechanisms of antigen presentation for
Neurology Neuroimmunology amp Neuroinflammation 9
ordf 2016 American Academy of Neurology Unauthorized reproduction of this article is prohibited
prevention of experimental autoimmune encephalomyeli-
tis J Immunol 20071782212ndash2220
50 Getts DR Turley DM Smith CE et al Tolerance
induced by apoptotic antigen-coupled leukocytes is
induced by PD-L11 and IL-10-producing splenic macro-
phages and maintained by T regulatory cells J Immunol
20111872405ndash2417
51 Luo X Pothoven KL McCarthy D et al ECDI-fixed allo-
geneic splenocytes induce donor-specific tolerance for long-
term survival of islet transplants via two distinct mechanisms
Proc Natl Acad Sci USA 200810514527ndash14532
52 Lutterotti A Yousef S Sputtek A et al Antigen-specific
tolerance by autologous myelin peptide-coupled cells
a phase 1 trial in multiple sclerosis Sci Transl Med
20135188ra75
53 Bielekova B SungMH KadomN Simon R McFarland HF
Martin R Expansion and functional relevance of high-avidity
myelin-specific CD41 T cells in multiple sclerosis
J Immunol 20041723893ndash3904
54 Jenkins MK Mueller D Schwartz RH et al Induction
and maintenance of anergy in mature T cells Adv Exp
Med Biol 1991292167ndash176
55 Peschl P Reindl M Schanda K Sospedra M
Martin R Lutterotti A Anti-myelin antibody re-
sponses following induction of antigen-specific toler-
ance with antigen-coupled cells J Neuroimmunol
201427512
56 Tuohy VK Yu M Yin L Kawczak JA Kinkel PR Regres-
sion and spreading of self-recognition during the develop-
ment of autoimmune demyelinating disease J Autoimmun
19991311ndash20
57 Vanderlugt CL Miller SD Epitope spreading in immune-
mediated diseases implications for immunotherapy Nat
Rev Immunol 2002285ndash95
58 Stromnes IM Schmitt TM Chapuis AG Hingorani SR
Greenberg PD Re-adapting T cells for cancer therapy
from mouse models to clinical trials Immunol Rev
2014257145ndash164
10 Neurology Neuroimmunology amp Neuroinflammation
ordf 2016 American Academy of Neurology Unauthorized reproduction of this article is prohibited
DOI 101212NXI000000000000027620163 Neurol Neuroimmunol Neuroinflamm
Larry Steinman Amit Bar-Or Jacinta M Behne et al Restoring immune tolerance in neuromyelitis optica Part I
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manufacturing process from one blood collection tobe used to generate sufficient drug for at least 1 yearof treatment
Considering the aggregate evidence a compellingcase can be made for the critical role of T cells inthe pathogenesis of NMOSD29 It follows thatreestablishment of immune tolerance to AQP4through vaccination with autologous attenuatedand idiotype-restricted T cells represents a promisingnew approach to immunotherapy in this disease
Dendritic cell vaccines Dendritic cells (DCs) presentantigens to immune cells including those with regula-tory and autoreactive function through major histo-compatibility complex (MHC) class I and IIrestriction Normally DCs present self-antigens butdo not drive autoreactive immune responses Thusautoreactive T cells are inactivated or anergizedbecause of low MHC expression inadequatecostimulation (eg inducing anergy) or tolerogeniccostimulation (eg PD-L1 [programed death ligand1] IL-10 etc) In addition DCs have important rolesin maintaining tolerance by promoting regulatorycells such as CD41 and CD81 Tregs and IL-10ndashproducing regulatory B cells37 Immature human DCsare readily generated in the laboratory from bloodmonocytes and can be engineered to maintaintolerogenic phenotypes Indeed DC-based therapieshave now entered clinical trials focusing on NMOSD(ClinicalTrialsgov identifier NCT02283671)
As a strategy for inducing immune tolerance inT1D DC administration has proven relatively safe38ndash42 Here granulocyte macrophagendashcolony stimulatingfactorIL-4 was used to generate immature CD11c1
DCs from monocytes in vitro Transition to a proin-flammatory DC phenotype is concerning when DCsare administered to human patients To mitigate thisconcern immature DCs can be treated in vitro withimmunosuppressive agents such as corticosteroids vita-min D3 andor IL-1043 before their use as tolerizingvaccines The resulting DCs exhibit durable tolerogenicphenotypes that prolong organ allograft survival inmice Notably this conditioning enhances the tolero-genic properties of DCs44 and induces high-level IL-10production which inhibit activated Th1 and Th17cells induce Tregs and suppress autoreactive B cellfunctions A related strategy involves loading of imma-ture DCs with antisense oligonucleotides targeting keycostimulatory molecules such as CD40 CD80 orCD86 Alternatively viral vectors encoding immuno-regulatory factors may potentiate tolerogenic DCs Ofnote the maintenance of DC immaturity also allowsthese cells to migrate into lymph nodes following anti-gen exposure enhancing their tolerogenic potential
Loading immature DCs with either native or syn-thetic peptides or whole antigens may also promote
antigen-specific tolerance through induction of regula-tory lymphocytes This strategy addresses multiple auto-antigens andor epitopes within a target antigen(s)Thus the goal is conditional presentation of self-antigenic epitopes by DCs to yield Treg inductionUsing the entire antigen affords presentation of a broad-er repertoire of peptides selected by relevant MHC al-leles and potentially avoids artificial epitope(s)selection In this respect preloading microparticlescontaining antigen might facilitate DC uptake andfavor tolerogenic phenotypes Of note early studiesin which rodents were vaccinated with autoantigen-loaded DCs demonstrated antigen-specific durableand effective tolerance These principles have beenapplied in human trials of T1D42 Advantages mayalso emerge from strategies in which DCs are al-lowed to antigen-load in situ
Small phase I clinical trials exploring DC vaccinesin autoimmune disease have largely focused on T1Dor rheumatoid arthritis Evidence to date suggests thattolerizing DC therapies are safe However larger trialswill be required to more thoroughly assess safety andefficacy Despite the great interest in further assessingDC vaccines in the prevention modulation and cur-ing of autoimmune diseases key questions remainThese include optimization of DC dosing vaccina-tion frequency (eg boosting) route of administra-tion and identification or validation of biomarkersfor assessing therapeutic efficacy
NMOSD appears to represent an ideal disease forDC therapy because a dominant and T cellndashrestrictedautoantigen (AQP4) has been identified If successfulthis approach should be used as early in the diseasecourse as is possible The rationale for this strategy isto prevent or ameliorate the expansion of inflamma-tory AQP4-restricted or other antigen-restrictedT lymphocytes thereby minimizing broader tissueinjury In addition such early intervention could inconcept minimize the potential for antigen spreadingor reversal of antigenic ignorance
Antigen-coupled presentation Antigen-specific toleriza-tion in animal models of disease has been pursued bya number of laboratory groups Among these modelsare those for experimental autoimmune encephalo-myelitis (EAE) Theiler virusndashinduced encephalitistolerance to organ transplantation and therapyfor severe allergy645ndash50 Use of peptide-coupled orchemically fixed antigen-presenting cells (APCs) byMiller et al6 has been one strategy Here unmodifiedsplenocytes are pulsed in vitro with a disease-relevantautoantigen(s) together with a coupling agent Thehost is immunized using these cells with the goal ofinducing tolerance to a target autoantigen(s) Thisapproach was effective in reducing disease activityin a model of relapsing-remitting EAE in SJL
Neurology Neuroimmunology amp Neuroinflammation 5
ordf 2016 American Academy of Neurology Unauthorized reproduction of this article is prohibited
mice4547 The strategy may also be attractive asprophylaxis for allotransplantation and the attenuationof alloreactive immune responses51 Key advantages ofthis approach may include (1) extensive experience inEAE and other autoimmune disease models (2) realisticpotential for targeting multiple autoantigens (3)acceptable safety profiles in preclinical studies (4) anapparent absence of epitope spreading and (5) positivepreliminary evidence for efficacy45 These attributesprovide a strong rationale for applying this approach toindividuals with antigenically characterized autoimmunediseases such as NMOSD
This strategy has been translated to human trialsusing unaltered autologous peripheral blood mono-nuclear cells (PBMCs) as APCs following their isola-tion by therapeutic leukapheresis52 In MS isolatedcells are first pulsed in vitro with a mixture of immu-nodominant myelin peptides that are identified astargets of the high-avidity myelin-specific T cellresponse53 A phase I trial was conducted in whichpatients received graded dosages of peptide-coupledand fixed autologous PBMCs52 Results suggested thisapproach was well tolerated and induced neitherimmune reactivity to myelin peptides nor triggereddisease relapses Rather the data demonstrated thatthe abundance of myelin-reactive T cells was substan-tially reduced52 Several putative mechanisms ofaction have been attributed to this strategy FirstAPC presentation of cross-linked peptide(s) can leadto anergy andor functional silencing of T cells result-ing from inadequate second-signaling4954 Here thedominant mechanisms are most likely related to Tcell recognition of peptide-coupled antigen com-plexes In this scenario T cells andor APCs undergoapoptosis and are phagocytosed by a special subset ofimmature DCs or macrophages in the splenic mar-ginal zone454950 These immature DCsmacrophagescan present an autoantigen peptide(s) in a tolerogenicmanner (eg IL-10) to pathogenic autoreactiveT cells Each of these pathways might help dampenautoimmunity Induction of Tregs is also likely criticalin suppressing autoreactivity45 Tolerization withpeptide-coupled fixed PBMCs undergoing apoptosisin vivo does not lead to the induction of anti-DNAautoantibodies55 The relevant mechanism(s) involvedin this immune tolerizing strategy and how it might beapplied to human diseases require further study
Despite promising results in animals humanstudies have been limited to demonstrating that theabundance of myelin-specific T cells is substantiallyreduced52 however this phase I study was not designedto elucidate specific mechanisms of action The abovetolerization approach with AQP4-coupled fixed APCscould in theory be readily adapted to NMOSDMoreover epitope spreading can occur in this disease56
and may be attenuated by this strategy (reviewed in
reference 57) Thus tolerization with the entireAQP4 holoprotein or derivative immunodominantpeptides may dampen autoreactivity against AQP4and secondary autoantigen targets providing clinicalbenefit Naturally technical challenges are likely to arisein these therapeutic strategies and their approach to theclinic Moreover specific biomarkers for determiningefficacy will be needed to unambiguously inform thetherapeutic benefit Nonetheless this approach holdspromise for restoring immune tolerance in NMOSDand other autoimmune conditions
T cell receptor engineering The ab TcR is the criticalantigen recognition determinant on CD41 and CD81
T cells conferring both antigen specificity and sensitiv-ity TcR diversity results from rearrangement of multi-ple genes (Figure 2) A key difference from antibodies isthe requirement that ab TcRs bind to peptide ligandsdisplayed through the APC MHC This MHC restric-tion focuses T cell activity against cells that bear therespective antigen This mechanism facilitates targetingto antigen-enriched anatomical sites such as lymphnodes and infected tissues while effector T cellspromote immune responses against foreign orendogenous autoantigens CD41 Tregs suppress theseresponses (part II)
Improved understanding of TcR-driven immunerecognition should yield strategies to harness thesecells in treating autoimmune diseases For exampleautoreactive TcR functions may be redirected tomodulate their disease-propagating phenotypes Bycomparison ongoing efforts to generate therapeuticautologous T cells targeting cancer involve engineer-ing TcRs specific to cancer peptideclass I MHC anti-gens58e1 These cells mature into effector T cells exvivo that are then administered to the patient Initialattempts involved CD81 T cell clones exhibiting rel-atively low affinities (KD range 10ndash100 mM)e1 How-ever subsequent engineering has yielded higheraffinity TcRs with KD ranges from 01 to 1 mMMoreover TcRs targeting class I antigens do notrequire the CD8 coreceptor and are thus capable ofrecruiting effector CD41 T cells against cancer cellsexpressing their cognate target antigene2 Conceiv-ably this approach might also prove useful in target-ing autoreactive cells To date TcR engineering hasbeen accomplished using several methodologiesincluding yeast and phage displaye3
Engineered TcRs can be delivered using a varietyof strategies These include administration as solubleagents via gene transfer or as adoptive T cell thera-pies Soluble TcRs will likely require affinities compa-rable to those of antibodies (KD in nM or pM range)and formatting as immunoglobulin fusions bispecificagents with anti-CD3e4 or as fusions with immuno-regulatory molecules such as cytokinese5 A high-
6 Neurology Neuroimmunology amp Neuroinflammation
ordf 2016 American Academy of Neurology Unauthorized reproduction of this article is prohibited
affinity TcR against a class II MHC fused to IL-10has been developed as a means for downregulatingautoimmune responsese6 Optimizing the tissue tar-geting of these constructs remains challenging
Adoptive T cell transfer of enriched Tregs has alsobeen used as a method of antigen-specific toleriza-tione7e8 Yet optimal delivery of antigen-specific sta-ble Tregs remains to be determined Moreover TcRaffinity and specificity of Tregse9 is less well charac-terized than those of effector T cells Several studieshave demonstrated that introducing class IndashrestrictedTcR into Tregs can redirect their specific activitiese10
and that TcRs with KDs of approximately 1 mM maybe of sufficient avidity to drive Treg activitye11 TcRsdirected against class I targets required lower affinitiesthan those in effector CD41 T cells that promoteTreg activity
Opportunities to generate mouse models and ther-apeutic T cells targeting AQP4 would appear to benumerouse12 For example in a mouse model animmunogenic response can be elicited with effectorT cells (CD41 andor CD81) expressing an AQP4-specific antibody (scFv)e13 fused to a chimeric antigenreceptor (CAR) Redirection of Tregs to suppresseffector cells could provide greater therapeutic poten-tial and utilize CARs directed against AQP4 Alter-natively conventional TcRs targeting AQP4 peptideMHC class I complexes such as human leukocyteantigen (HLA)-A2 (which are expressed by 40 ofthe population) might prove effective Possible advan-tages of CARs include their potential use of scFv fromexisting monoclonal antibodiese13 Furthermore thesame scFv used in effector T cells to initiate patho-genic responses could instead be engineered to a Tregphenotype Here scFv affinity could be optimizedusing single-site complementarity determining re-gions mutations Treg signaling and activity woulddepend on the CAR signaling domain specificityand affinity as well as density of AQP4 displayedon target cells Animal models that more preciselyrecapitulate human disease would represent signifi-cant advances toward these goals
The TcR approach offers potential advantage overscFvCAR including direct targeting of Treg activityto class Indashbearing tissues and regional lymph nodeswhere AQP4 might be cross-presented by MHCOstensibly this strategy might arrest or reversecross-presentation of AQP4 antigens by class IIMHC which may occur early in the developmentof NMOSDe14 If so this approach may also mitigateepitope spreading The generation of TcRs againstAQP4 peptides that bind to HLA-A2 would be rela-tively straightforward given the extensive knowledgeof its binding motifs and the likelihood that AQP4contains cognate epitopese15 The TcR affinity in thisscenario may prove inadequate but such limitations
might be overcome through the generation of CARmutants
AQP4 antigenndashspecific approaches must addressissues well beyond TcR and CAR affinity Theseinclude untoward conversion of Tregs to pathogenicphenotypes such as those exhibiting either Th1 orTh17 paradigmse16 accurate tissue targeting and pro-motion of memory and stabilitye17e18 It is possiblethat the same tolerance checkpoints currently beingconsidered for rapid deletion of undesirable T cellstransferred in cancer could be applied to NMOSD
SUMMARY AND PROSPECTUS PART I Immunetolerization attempts to restore central or peripheral tol-erance thereby suppressing autoimmune diseases Thereis reason for optimism that this approach might offersubstantial clinical benefit to individuals with NMOSD DNA vaccination T cell vaccination tolerogenicDCs and peptide-coupling strategies have advancedto clinic trials Other promising approaches (egCAR-based or Treg-based therapeutics) are in earlierdevelopmental stages in autoimmune diseases Yetdefining target cell lineages to yield durable efficacyand determining stage of disease development optimalfor intervention remain crucial ongoing challenges Pastfailures using similar strategies have provided criticalinsights that despite the complexities of existing andemerging technologies for promoting tolerance shouldilluminate the pathway forward If so such advances intreatment could benefit individuals with NMOSDand other autoimmune diseases including arrestingdisease progression prolonging intervals to remissionenabling tissue repair and ultimately avoiding chronicimmunosuppressive therapy
AUTHOR CONTRIBUTIONSLarry Steinman draftingrevising the manuscript Amit Bar-Or drafting
revising the manuscript Jacinta M Behne revising the manuscript
Daniel Benitez-Ribas draftingrevising the manuscript Peter S Chin
draftingrevising the manuscript Michael Clare-Salzler draftingrevising
the manuscript Donald Healey draftingrevising the manuscript James
I Kim draftingrevising the manuscript David M Kranz draftingrevis-
ing the manuscript Andreas Lutterotti draftingrevising the manuscript
Roland Martin draftingrevising the manuscript Sven Schippling draft-
ingrevising the manuscript Pablo Villoslada draftingrevising the man-
uscript Cheng-Hong Wei draftingrevising the manuscript Howard L
Weiner draftingrevising the manuscript Scott S Zamvil draftingrevis-
ing the manuscript Michael R Yeaman draftingrevising the manu-
script study concept or design analysis or interpretation of data
accepts responsibility for conduct of research and will give final approval
Terry J Smith draftingrevising the manuscript study concept or design
analysis or interpretation of data accepts responsibility for conduct of
research and will give final approval
ACKNOWLEDGMENTWe thank Dr Gerald Nepom Dr Philip Bernstein and Dr William St
Clair for their helpful comments
STUDY FUNDINGThis work was supported in part by the Guthy-Jackson Charitable
Foundation
Neurology Neuroimmunology amp Neuroinflammation 7
ordf 2016 American Academy of Neurology Unauthorized reproduction of this article is prohibited
DISCLOSUREL Steinman served on the scientific advisory board for Novartis Recep-
tos Atreca Tolerion Teva received travel funding andor speaker hon-
oraria from Biogen Bayhill Bayer Celgene Receptos is on the editorial
board for Multiple Sclerosis Journal Proceedings of the National Academy of
Science holds a patents for antigen-specific tolerance has a patent pend-
ing for cytokines and type 1 interferons is on the speakers bureau for
EMD Serono received research support from NIH has stock options
and board membership in Tolerion is on the board of directors for
BioAtla A Bar-Or is on the scientific advisory board for Dionix
Receptos-Celgene RocheGenentech Novartis GSK Guthy-Jackson
Greater Good Foundation Immune Tolerance Network received travel
funding andor speaker honoraria from Receptos-Celgene RocheGen-
entech Novartis Sanofi-Genzyme GSK served on the editorial board
for Neurologyreg Clinical and Experimental Neuroimmunology consulted
for DioGenix Receptos-Celgene RocheGenentech Novartis Sanofi-
Genzyme GSK received research support from Novartis Sanofi-
Genzyme JM Behne and D Benitez-Ribas report no disclosures PS
Chin served as the medical director for Genentech and Novartis Pharma-
ceuticals Corp M Clare-Salzler received research support from NIH
NIAID D Healy has a patent pending for the treatment of B cellndash
mediated autoimmunities with T cell vaccination has been CSO for
Opexa Therapeutics received research support from and holds stock
stock options in Opexa Therapeutics JI Kim is an associate editor for
the Journal of Negative Results in Biomedicine is employed by Unum
Therapeutics DM Kranz has patents and patents pending in the areas
of yeast display and T cell receptor engineering and receives license fee
payments from them consulted for AbbVie received research support
from NIH Melanoma Research Alliance A Lutterotti served on the
scientific advisory board for Bayer Biogen Novartis Genzyme received
travel support from European Charcot Foundation Fundacio GAEM
holds a patent with the University of Zurich received research support
from Wyss Translational Center Austrian MS Society R Martin served
on the scientific advisory board for Biogen Merck Serono Teva
Genzyme Sanofi-Aventis CellProtect Neuway received speaker hono-
raria from Biogen Merck Serono Novartis Roche Genzyme holds
a patent for the therapeutic efficacy of anto-CD25 monoclonal antibody
treatment in combination with IFN-b in MS consulted for Myelin
Repair Foundation The Weatherall Institute of Molecular Studies Uni-
versity of Oxford the Hertie Foundation is a member of the Kuratorium
of the Jung Foundation for Science received research support from
Novartis Biogen Swiss National Science Foundation European Union
Seventh Framework Program European Research Council S Schippling
served on the scientific advisory board for Bayer Healthcare Biogen
Merck Serono Novartis Sanofi-Genzyme TEVA received travel fund-
ing andor speaker honoraria from Bayer Healthcare Biogen Merck
Serono Novartis Sanofi-Aventis TEVA is an associate editor for Fron-
tiers in Neurology holds a patent for therapeutic vaccination in PML
using VP1 and Il7 received research support from Sanofi-Genzyme
Novartis University of Zurich Betty and David Koetser Foundation
for Brain Research Swiss Multiple Sclerosis Society P Villoslada received
travel funding andor speaker honoraria from Novartis Roche Genzyme
served as an academic editor for PLoS One served on the editorial board
for Neurology and Therapy Current Treatment Options in Neurology Mul-
tiple Sclerosis and Demyelinating Disorders holds a patent for methylthioa-
denosine for the treatment of MS Agnostic neurotrophic compounds for
the treatment of brain diseases Gene signature pattern as a biomarker for
MS Algorithm for quantifying fractal dimension in brain MRI received
research support from Novartis Roche Genzyme Instituto de Salud
Carlos III European Commission National MS Society Fundacion
Maraton TV3 holds stock or stock options in Bionure Inc Spire Bio-
ventures Mint-Labs C-H Wei reports no disclosures HL Weiner
served on the scientific advisory board for the Guthy-Jackson Charitable
Foundation Teva Pharmaceutical Industries Biogen Idec Novartis
Sanofi-Aventis consulted for Therapix Biogen Novartis Serono Teva
Sanofi received research support from National Multiple Sclerosis Soci-
ety S Zamvil served on the scientific advisory board for BioMS Teva
Pharmaceuticals Eli Lilly and Co Myelin Repair Foundation is deputy
editor for Neurologyreg Neuroimmunology amp Neuroinflammation consulted
for Biogen Idec Teva Neuroscience EMD Serono Genzyme Novartis
Roche is on the speakers bureau for Advanced Health Media Biogen
received research support from NIH NMSS Alexander M and June L
Maisin Foundation MR Yeaman is on the scientific advisory board for
Guthy-Jackson Charitable Foundation served as an associate editor for
PLoS Pathogens holds patents for Vaccines targeting drug-resistant patho-
gens immunotherapies targeting drug-resistant pathogens novel anti-
infective biological therapeutics novel anti-infective small molecules
novel biological regulating programmed cell death consulted for
Guthy-Jackson Charitable Foundation received research support from
NovaDigm Therapeutics Inc Metacin Inc US Department of Defense
NIH holds stock or stock options for NovaDigm Therapeutics Inc Meta-
cin Inc receives license fee and royalty payments from NovaDigm Thera-
peutics TJ Smith received research support from NIH University of South
Denmark Bell Charitable Foundation RPB Foundation is a member of the
Guthy-Jackson Charitable Foundation scientific advisory board and holds
patents covering the blockade of insulin-like growth factor receptor-l in
autoimmune diseases Go to Neurologyorgnn for full disclosure forms
Received February 12 2016 Accepted in final form July 15 2016
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ization of hyperacute rejection in a transgenic mouse graft
versus host model Transplantation 201294234ndash240
8 Castells M Rapid desensitization of hypersensitivity reactions
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9 Fousteri G Bresson D von Herrath M Rational develop-
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10 Turley DM Miller SD Prospects for antigen-specific tol-
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11 Mori K Yamanishi H Ikeda Y et al Oral administration
of carbonic anhydrase I ameliorates murine experimental
colitis induced by Foxp3-CD41CD252 T cells J Leukoc
Biol 201393963ndash972
12 Genain CP Abel K Belmar N et al Late complications of
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13 Dolgin E The inverse of immunity Nat Med 201016
740ndash743
14 Steinman L Inverse vaccination the opposite of Jennerrsquos
concept for therapy of autoimmunity J Intern Med 2010
267441ndash451
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ordf 2016 American Academy of Neurology Unauthorized reproduction of this article is prohibited
15 Hinson SR McKeon A Lennon VA Neurological auto-
immunity targeting aquaporin-4 Neuroscience 2010168
1009ndash1018
16 Sato DK Callegaro D de Haidar Jorge FM et al Cere-
brospinal fluid aquaporin-4 antibody levels in neuromye-
litis optica attacks Ann Neurol 201476305ndash309
17 Steinman L The re-emergence of antigen-specific toler-
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18 Roep BO Solvason N Gottlieb PA et al Plasmid
encoded proinsulin preserves C-peptide while specifically
reducing proinsulin specific CD8 T cells in type 1 diabe-
tes Sci Transl Med 20135191ra82
19 Feldmann M Steinman L Design of effective immuno-
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20 Garren H Robinson WH Krasulovaacute E et al Phase 2 trial
of a DNA vaccine encoding myelin basic protein for mul-
tiple sclerosis Ann Neurol 200863611ndash620
21 Bar-Or A Vollmer T Antel J et al Induction of antigen-
specific tolerance in multiple sclerosis after immunization
with DNA encoding myelin basic protein in a randomized
placebo-controlled phase 12 trial Arch Neurol 200764
1407ndash1415
22 Selmi C Autoimmunity in 2012 Clin Rev Allergy
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23 Martin A Tisch RM Getts DR Manipulating T cell-
mediated pathology targets and functions of monoclonal
antibody immunotherapy Clin Immunol 2013148
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24 Salvana EM Salata RA Infectious complications associ-
ated with monoclonal antibodies and related small mole-
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25 Madakamutil LT Maricic I Sercarz EE Kumar V
Immunodominance in the TCR repertoire of a TCR
peptide-specific CD41 Treg population that controls
experimental autoimmune encephalomyelitis J Immunol
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26 Buckner JH Mechanisms of impaired regulation by CD4
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27 Linhares UC Schiavoni PB Barros PO et al The ex vivo
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28 Pohl M Fisher MT Mader S et al Pathogenic T cells
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29 Varrin-Doyer M Spencer CM Schulze-Topphoff U et al
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30 Kumar V Sercarz EE The involvement of T cell receptor
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31 Zhang J T cell vaccination as an immunotherapy for
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32 Huang X Wu H Lu Q The mechanisms and applica-
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33 Fontana R Bregni M Cipponi A et al Peripheral blood
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antigen MAGE-A3 induce antitumor immune responses
in cancer patients Blood 20091131651ndash1660
34 Fox E Wynn D Cohan S Rill D McGuire D
Markowitz C A randomized clinical trial of autolo-
gous T-cell therapy in multiple sclerosis subset anal-
ysis and implications for trial design Mult Scler 2012
18843ndash852
35 Chen G Li N Zang YC et al Vaccination with selected
synovial T cells in rheumatoid arthritis Arthritis Rheum
200756453ndash463
36 Li ZG Mu R Dai ZP Gao XM T cell vaccination in
systemic lupus erythematosus with autologous activated T
cells Lupus 200514884ndash889
37 Leavy O Dendritic cells tracing the origins of cDCs Nat
Rev Immunol 201313703
38 Banchereau J Palucka AK Dendritic cells as therapeutic
vaccines against cancer Nat Rev Immunol 20055296ndash
306
39 Vacchelli E Vitale I Eggermont A et al Trial watch
dendritic cell-based interventions for cancer therapy
Oncoimmunology 201211111ndash1134
40 Mantia-Smaldone GM Chu CS A review of dendritic cell
therapy for cancer progress and challenges BioDrugs
201327453ndash468
41 Segovia-Gamboa N Rodriguez-Arellano ME Rangel-
Cruz R et al Tolerogenic dendritic cells induce antigen-
specific hyporesponsiveness in insulin- and glutamic acid
decarboxylase 65-autoreactive T lymphocytes from type 1
diabetic patients Clin Immunol 201415472ndash83
42 Giannoukakis N Phillips B Finegold D Harnaha J
Trucco M Phase I (safety) study of autologous tolerogenic
dendritic cells in type 1 diabetic patients Diabetes Care
2011342026ndash2032
43 Boks MA Kager-Groenland J Haasjes MSP Zwaginga JJ
van Ham SM ten Brinke A IL-10-generated tolerogenic
dendritic cells are optimal for functional regulatory T cell
induction- a comparative study on human clinical-
applicable DC Clin Immunol 2012142332ndash342
44 Saito M Nagasaw M Takada H et al Defective IL-10
signaling in hyper-IgE syndrome results in impaired gen-
eration of tolerogenic dendritic cells and induced regula-
tory T cells J Exp Med 2011208235ndash249
45 Miller SD Turley DM Podojil JR Antigen-specific tol-
erance strategies for the prevention and treatment of auto-
immune disease Nat Rev Immunol 20077665ndash677
46 Karpus WJ Pope JG Peterson JD Dal Canto MC
Miller SD Inhibition of Theilerrsquos virus-mediated demye-
lination by peripheral immune tolerance induction
J Immunol 1995155947ndash957
47 Kennedy KJ Smith WS Miller SD Karpus WJ Induc-
tion of antigen-specific tolerance for the treatment of
ongoing relapsing autoimmune encephalomyelitis a com-
parison between oral and peripheral tolerance J Immunol
19971591036ndash1044
48 Kennedy MK Tan LJ Dal Canto MC et al Inhibition of
murine relapsing experimental autoimmune encephalomy-
elitis by immune tolerance to proteolipid protein and its
encephalitogenic peptides J Immunol 1990144909ndash
915
49 Turley DM Miller SD Peripheral tolerance induction
using ethylenecarbodiimide-fixed APCs uses both direct
and indirect mechanisms of antigen presentation for
Neurology Neuroimmunology amp Neuroinflammation 9
ordf 2016 American Academy of Neurology Unauthorized reproduction of this article is prohibited
prevention of experimental autoimmune encephalomyeli-
tis J Immunol 20071782212ndash2220
50 Getts DR Turley DM Smith CE et al Tolerance
induced by apoptotic antigen-coupled leukocytes is
induced by PD-L11 and IL-10-producing splenic macro-
phages and maintained by T regulatory cells J Immunol
20111872405ndash2417
51 Luo X Pothoven KL McCarthy D et al ECDI-fixed allo-
geneic splenocytes induce donor-specific tolerance for long-
term survival of islet transplants via two distinct mechanisms
Proc Natl Acad Sci USA 200810514527ndash14532
52 Lutterotti A Yousef S Sputtek A et al Antigen-specific
tolerance by autologous myelin peptide-coupled cells
a phase 1 trial in multiple sclerosis Sci Transl Med
20135188ra75
53 Bielekova B SungMH KadomN Simon R McFarland HF
Martin R Expansion and functional relevance of high-avidity
myelin-specific CD41 T cells in multiple sclerosis
J Immunol 20041723893ndash3904
54 Jenkins MK Mueller D Schwartz RH et al Induction
and maintenance of anergy in mature T cells Adv Exp
Med Biol 1991292167ndash176
55 Peschl P Reindl M Schanda K Sospedra M
Martin R Lutterotti A Anti-myelin antibody re-
sponses following induction of antigen-specific toler-
ance with antigen-coupled cells J Neuroimmunol
201427512
56 Tuohy VK Yu M Yin L Kawczak JA Kinkel PR Regres-
sion and spreading of self-recognition during the develop-
ment of autoimmune demyelinating disease J Autoimmun
19991311ndash20
57 Vanderlugt CL Miller SD Epitope spreading in immune-
mediated diseases implications for immunotherapy Nat
Rev Immunol 2002285ndash95
58 Stromnes IM Schmitt TM Chapuis AG Hingorani SR
Greenberg PD Re-adapting T cells for cancer therapy
from mouse models to clinical trials Immunol Rev
2014257145ndash164
10 Neurology Neuroimmunology amp Neuroinflammation
ordf 2016 American Academy of Neurology Unauthorized reproduction of this article is prohibited
DOI 101212NXI000000000000027620163 Neurol Neuroimmunol Neuroinflamm
Larry Steinman Amit Bar-Or Jacinta M Behne et al Restoring immune tolerance in neuromyelitis optica Part I
This information is current as of September 7 2016
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References httpnnneurologyorgcontent35e276fullhtmlref-list-1
This article cites 58 articles 18 of which you can access for free at
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is an official journal of the American Academy of NeurologyNeurol Neuroimmunol Neuroinflamm
mice4547 The strategy may also be attractive asprophylaxis for allotransplantation and the attenuationof alloreactive immune responses51 Key advantages ofthis approach may include (1) extensive experience inEAE and other autoimmune disease models (2) realisticpotential for targeting multiple autoantigens (3)acceptable safety profiles in preclinical studies (4) anapparent absence of epitope spreading and (5) positivepreliminary evidence for efficacy45 These attributesprovide a strong rationale for applying this approach toindividuals with antigenically characterized autoimmunediseases such as NMOSD
This strategy has been translated to human trialsusing unaltered autologous peripheral blood mono-nuclear cells (PBMCs) as APCs following their isola-tion by therapeutic leukapheresis52 In MS isolatedcells are first pulsed in vitro with a mixture of immu-nodominant myelin peptides that are identified astargets of the high-avidity myelin-specific T cellresponse53 A phase I trial was conducted in whichpatients received graded dosages of peptide-coupledand fixed autologous PBMCs52 Results suggested thisapproach was well tolerated and induced neitherimmune reactivity to myelin peptides nor triggereddisease relapses Rather the data demonstrated thatthe abundance of myelin-reactive T cells was substan-tially reduced52 Several putative mechanisms ofaction have been attributed to this strategy FirstAPC presentation of cross-linked peptide(s) can leadto anergy andor functional silencing of T cells result-ing from inadequate second-signaling4954 Here thedominant mechanisms are most likely related to Tcell recognition of peptide-coupled antigen com-plexes In this scenario T cells andor APCs undergoapoptosis and are phagocytosed by a special subset ofimmature DCs or macrophages in the splenic mar-ginal zone454950 These immature DCsmacrophagescan present an autoantigen peptide(s) in a tolerogenicmanner (eg IL-10) to pathogenic autoreactiveT cells Each of these pathways might help dampenautoimmunity Induction of Tregs is also likely criticalin suppressing autoreactivity45 Tolerization withpeptide-coupled fixed PBMCs undergoing apoptosisin vivo does not lead to the induction of anti-DNAautoantibodies55 The relevant mechanism(s) involvedin this immune tolerizing strategy and how it might beapplied to human diseases require further study
Despite promising results in animals humanstudies have been limited to demonstrating that theabundance of myelin-specific T cells is substantiallyreduced52 however this phase I study was not designedto elucidate specific mechanisms of action The abovetolerization approach with AQP4-coupled fixed APCscould in theory be readily adapted to NMOSDMoreover epitope spreading can occur in this disease56
and may be attenuated by this strategy (reviewed in
reference 57) Thus tolerization with the entireAQP4 holoprotein or derivative immunodominantpeptides may dampen autoreactivity against AQP4and secondary autoantigen targets providing clinicalbenefit Naturally technical challenges are likely to arisein these therapeutic strategies and their approach to theclinic Moreover specific biomarkers for determiningefficacy will be needed to unambiguously inform thetherapeutic benefit Nonetheless this approach holdspromise for restoring immune tolerance in NMOSDand other autoimmune conditions
T cell receptor engineering The ab TcR is the criticalantigen recognition determinant on CD41 and CD81
T cells conferring both antigen specificity and sensitiv-ity TcR diversity results from rearrangement of multi-ple genes (Figure 2) A key difference from antibodies isthe requirement that ab TcRs bind to peptide ligandsdisplayed through the APC MHC This MHC restric-tion focuses T cell activity against cells that bear therespective antigen This mechanism facilitates targetingto antigen-enriched anatomical sites such as lymphnodes and infected tissues while effector T cellspromote immune responses against foreign orendogenous autoantigens CD41 Tregs suppress theseresponses (part II)
Improved understanding of TcR-driven immunerecognition should yield strategies to harness thesecells in treating autoimmune diseases For exampleautoreactive TcR functions may be redirected tomodulate their disease-propagating phenotypes Bycomparison ongoing efforts to generate therapeuticautologous T cells targeting cancer involve engineer-ing TcRs specific to cancer peptideclass I MHC anti-gens58e1 These cells mature into effector T cells exvivo that are then administered to the patient Initialattempts involved CD81 T cell clones exhibiting rel-atively low affinities (KD range 10ndash100 mM)e1 How-ever subsequent engineering has yielded higheraffinity TcRs with KD ranges from 01 to 1 mMMoreover TcRs targeting class I antigens do notrequire the CD8 coreceptor and are thus capable ofrecruiting effector CD41 T cells against cancer cellsexpressing their cognate target antigene2 Conceiv-ably this approach might also prove useful in target-ing autoreactive cells To date TcR engineering hasbeen accomplished using several methodologiesincluding yeast and phage displaye3
Engineered TcRs can be delivered using a varietyof strategies These include administration as solubleagents via gene transfer or as adoptive T cell thera-pies Soluble TcRs will likely require affinities compa-rable to those of antibodies (KD in nM or pM range)and formatting as immunoglobulin fusions bispecificagents with anti-CD3e4 or as fusions with immuno-regulatory molecules such as cytokinese5 A high-
6 Neurology Neuroimmunology amp Neuroinflammation
ordf 2016 American Academy of Neurology Unauthorized reproduction of this article is prohibited
affinity TcR against a class II MHC fused to IL-10has been developed as a means for downregulatingautoimmune responsese6 Optimizing the tissue tar-geting of these constructs remains challenging
Adoptive T cell transfer of enriched Tregs has alsobeen used as a method of antigen-specific toleriza-tione7e8 Yet optimal delivery of antigen-specific sta-ble Tregs remains to be determined Moreover TcRaffinity and specificity of Tregse9 is less well charac-terized than those of effector T cells Several studieshave demonstrated that introducing class IndashrestrictedTcR into Tregs can redirect their specific activitiese10
and that TcRs with KDs of approximately 1 mM maybe of sufficient avidity to drive Treg activitye11 TcRsdirected against class I targets required lower affinitiesthan those in effector CD41 T cells that promoteTreg activity
Opportunities to generate mouse models and ther-apeutic T cells targeting AQP4 would appear to benumerouse12 For example in a mouse model animmunogenic response can be elicited with effectorT cells (CD41 andor CD81) expressing an AQP4-specific antibody (scFv)e13 fused to a chimeric antigenreceptor (CAR) Redirection of Tregs to suppresseffector cells could provide greater therapeutic poten-tial and utilize CARs directed against AQP4 Alter-natively conventional TcRs targeting AQP4 peptideMHC class I complexes such as human leukocyteantigen (HLA)-A2 (which are expressed by 40 ofthe population) might prove effective Possible advan-tages of CARs include their potential use of scFv fromexisting monoclonal antibodiese13 Furthermore thesame scFv used in effector T cells to initiate patho-genic responses could instead be engineered to a Tregphenotype Here scFv affinity could be optimizedusing single-site complementarity determining re-gions mutations Treg signaling and activity woulddepend on the CAR signaling domain specificityand affinity as well as density of AQP4 displayedon target cells Animal models that more preciselyrecapitulate human disease would represent signifi-cant advances toward these goals
The TcR approach offers potential advantage overscFvCAR including direct targeting of Treg activityto class Indashbearing tissues and regional lymph nodeswhere AQP4 might be cross-presented by MHCOstensibly this strategy might arrest or reversecross-presentation of AQP4 antigens by class IIMHC which may occur early in the developmentof NMOSDe14 If so this approach may also mitigateepitope spreading The generation of TcRs againstAQP4 peptides that bind to HLA-A2 would be rela-tively straightforward given the extensive knowledgeof its binding motifs and the likelihood that AQP4contains cognate epitopese15 The TcR affinity in thisscenario may prove inadequate but such limitations
might be overcome through the generation of CARmutants
AQP4 antigenndashspecific approaches must addressissues well beyond TcR and CAR affinity Theseinclude untoward conversion of Tregs to pathogenicphenotypes such as those exhibiting either Th1 orTh17 paradigmse16 accurate tissue targeting and pro-motion of memory and stabilitye17e18 It is possiblethat the same tolerance checkpoints currently beingconsidered for rapid deletion of undesirable T cellstransferred in cancer could be applied to NMOSD
SUMMARY AND PROSPECTUS PART I Immunetolerization attempts to restore central or peripheral tol-erance thereby suppressing autoimmune diseases Thereis reason for optimism that this approach might offersubstantial clinical benefit to individuals with NMOSD DNA vaccination T cell vaccination tolerogenicDCs and peptide-coupling strategies have advancedto clinic trials Other promising approaches (egCAR-based or Treg-based therapeutics) are in earlierdevelopmental stages in autoimmune diseases Yetdefining target cell lineages to yield durable efficacyand determining stage of disease development optimalfor intervention remain crucial ongoing challenges Pastfailures using similar strategies have provided criticalinsights that despite the complexities of existing andemerging technologies for promoting tolerance shouldilluminate the pathway forward If so such advances intreatment could benefit individuals with NMOSDand other autoimmune diseases including arrestingdisease progression prolonging intervals to remissionenabling tissue repair and ultimately avoiding chronicimmunosuppressive therapy
AUTHOR CONTRIBUTIONSLarry Steinman draftingrevising the manuscript Amit Bar-Or drafting
revising the manuscript Jacinta M Behne revising the manuscript
Daniel Benitez-Ribas draftingrevising the manuscript Peter S Chin
draftingrevising the manuscript Michael Clare-Salzler draftingrevising
the manuscript Donald Healey draftingrevising the manuscript James
I Kim draftingrevising the manuscript David M Kranz draftingrevis-
ing the manuscript Andreas Lutterotti draftingrevising the manuscript
Roland Martin draftingrevising the manuscript Sven Schippling draft-
ingrevising the manuscript Pablo Villoslada draftingrevising the man-
uscript Cheng-Hong Wei draftingrevising the manuscript Howard L
Weiner draftingrevising the manuscript Scott S Zamvil draftingrevis-
ing the manuscript Michael R Yeaman draftingrevising the manu-
script study concept or design analysis or interpretation of data
accepts responsibility for conduct of research and will give final approval
Terry J Smith draftingrevising the manuscript study concept or design
analysis or interpretation of data accepts responsibility for conduct of
research and will give final approval
ACKNOWLEDGMENTWe thank Dr Gerald Nepom Dr Philip Bernstein and Dr William St
Clair for their helpful comments
STUDY FUNDINGThis work was supported in part by the Guthy-Jackson Charitable
Foundation
Neurology Neuroimmunology amp Neuroinflammation 7
ordf 2016 American Academy of Neurology Unauthorized reproduction of this article is prohibited
DISCLOSUREL Steinman served on the scientific advisory board for Novartis Recep-
tos Atreca Tolerion Teva received travel funding andor speaker hon-
oraria from Biogen Bayhill Bayer Celgene Receptos is on the editorial
board for Multiple Sclerosis Journal Proceedings of the National Academy of
Science holds a patents for antigen-specific tolerance has a patent pend-
ing for cytokines and type 1 interferons is on the speakers bureau for
EMD Serono received research support from NIH has stock options
and board membership in Tolerion is on the board of directors for
BioAtla A Bar-Or is on the scientific advisory board for Dionix
Receptos-Celgene RocheGenentech Novartis GSK Guthy-Jackson
Greater Good Foundation Immune Tolerance Network received travel
funding andor speaker honoraria from Receptos-Celgene RocheGen-
entech Novartis Sanofi-Genzyme GSK served on the editorial board
for Neurologyreg Clinical and Experimental Neuroimmunology consulted
for DioGenix Receptos-Celgene RocheGenentech Novartis Sanofi-
Genzyme GSK received research support from Novartis Sanofi-
Genzyme JM Behne and D Benitez-Ribas report no disclosures PS
Chin served as the medical director for Genentech and Novartis Pharma-
ceuticals Corp M Clare-Salzler received research support from NIH
NIAID D Healy has a patent pending for the treatment of B cellndash
mediated autoimmunities with T cell vaccination has been CSO for
Opexa Therapeutics received research support from and holds stock
stock options in Opexa Therapeutics JI Kim is an associate editor for
the Journal of Negative Results in Biomedicine is employed by Unum
Therapeutics DM Kranz has patents and patents pending in the areas
of yeast display and T cell receptor engineering and receives license fee
payments from them consulted for AbbVie received research support
from NIH Melanoma Research Alliance A Lutterotti served on the
scientific advisory board for Bayer Biogen Novartis Genzyme received
travel support from European Charcot Foundation Fundacio GAEM
holds a patent with the University of Zurich received research support
from Wyss Translational Center Austrian MS Society R Martin served
on the scientific advisory board for Biogen Merck Serono Teva
Genzyme Sanofi-Aventis CellProtect Neuway received speaker hono-
raria from Biogen Merck Serono Novartis Roche Genzyme holds
a patent for the therapeutic efficacy of anto-CD25 monoclonal antibody
treatment in combination with IFN-b in MS consulted for Myelin
Repair Foundation The Weatherall Institute of Molecular Studies Uni-
versity of Oxford the Hertie Foundation is a member of the Kuratorium
of the Jung Foundation for Science received research support from
Novartis Biogen Swiss National Science Foundation European Union
Seventh Framework Program European Research Council S Schippling
served on the scientific advisory board for Bayer Healthcare Biogen
Merck Serono Novartis Sanofi-Genzyme TEVA received travel fund-
ing andor speaker honoraria from Bayer Healthcare Biogen Merck
Serono Novartis Sanofi-Aventis TEVA is an associate editor for Fron-
tiers in Neurology holds a patent for therapeutic vaccination in PML
using VP1 and Il7 received research support from Sanofi-Genzyme
Novartis University of Zurich Betty and David Koetser Foundation
for Brain Research Swiss Multiple Sclerosis Society P Villoslada received
travel funding andor speaker honoraria from Novartis Roche Genzyme
served as an academic editor for PLoS One served on the editorial board
for Neurology and Therapy Current Treatment Options in Neurology Mul-
tiple Sclerosis and Demyelinating Disorders holds a patent for methylthioa-
denosine for the treatment of MS Agnostic neurotrophic compounds for
the treatment of brain diseases Gene signature pattern as a biomarker for
MS Algorithm for quantifying fractal dimension in brain MRI received
research support from Novartis Roche Genzyme Instituto de Salud
Carlos III European Commission National MS Society Fundacion
Maraton TV3 holds stock or stock options in Bionure Inc Spire Bio-
ventures Mint-Labs C-H Wei reports no disclosures HL Weiner
served on the scientific advisory board for the Guthy-Jackson Charitable
Foundation Teva Pharmaceutical Industries Biogen Idec Novartis
Sanofi-Aventis consulted for Therapix Biogen Novartis Serono Teva
Sanofi received research support from National Multiple Sclerosis Soci-
ety S Zamvil served on the scientific advisory board for BioMS Teva
Pharmaceuticals Eli Lilly and Co Myelin Repair Foundation is deputy
editor for Neurologyreg Neuroimmunology amp Neuroinflammation consulted
for Biogen Idec Teva Neuroscience EMD Serono Genzyme Novartis
Roche is on the speakers bureau for Advanced Health Media Biogen
received research support from NIH NMSS Alexander M and June L
Maisin Foundation MR Yeaman is on the scientific advisory board for
Guthy-Jackson Charitable Foundation served as an associate editor for
PLoS Pathogens holds patents for Vaccines targeting drug-resistant patho-
gens immunotherapies targeting drug-resistant pathogens novel anti-
infective biological therapeutics novel anti-infective small molecules
novel biological regulating programmed cell death consulted for
Guthy-Jackson Charitable Foundation received research support from
NovaDigm Therapeutics Inc Metacin Inc US Department of Defense
NIH holds stock or stock options for NovaDigm Therapeutics Inc Meta-
cin Inc receives license fee and royalty payments from NovaDigm Thera-
peutics TJ Smith received research support from NIH University of South
Denmark Bell Charitable Foundation RPB Foundation is a member of the
Guthy-Jackson Charitable Foundation scientific advisory board and holds
patents covering the blockade of insulin-like growth factor receptor-l in
autoimmune diseases Go to Neurologyorgnn for full disclosure forms
Received February 12 2016 Accepted in final form July 15 2016
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ability and retention rate of azathioprine in 103 aquaporin-4
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6 Miller SD Wetzig RP Claman HN The induction of
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7 Oh S Schwartz RH Singh NJ Development and toler-
ization of hyperacute rejection in a transgenic mouse graft
versus host model Transplantation 201294234ndash240
8 Castells M Rapid desensitization of hypersensitivity reactions
to chemotherapy agents Curr Drug Saf 20061243ndash251
9 Fousteri G Bresson D von Herrath M Rational develop-
ment of antigen-specific therapies for type 1 diabetes Adv
Exp Med Biol 2007601313ndash319
10 Turley DM Miller SD Prospects for antigen-specific tol-
erance based therapies for the treatment of multiple scle-
rosis Results Probl Cell Differ 201051217ndash235
11 Mori K Yamanishi H Ikeda Y et al Oral administration
of carbonic anhydrase I ameliorates murine experimental
colitis induced by Foxp3-CD41CD252 T cells J Leukoc
Biol 201393963ndash972
12 Genain CP Abel K Belmar N et al Late complications of
immune deviation therapy in a nonhuman primate Sci-
ence 19962742054ndash2057
13 Dolgin E The inverse of immunity Nat Med 201016
740ndash743
14 Steinman L Inverse vaccination the opposite of Jennerrsquos
concept for therapy of autoimmunity J Intern Med 2010
267441ndash451
8 Neurology Neuroimmunology amp Neuroinflammation
ordf 2016 American Academy of Neurology Unauthorized reproduction of this article is prohibited
15 Hinson SR McKeon A Lennon VA Neurological auto-
immunity targeting aquaporin-4 Neuroscience 2010168
1009ndash1018
16 Sato DK Callegaro D de Haidar Jorge FM et al Cere-
brospinal fluid aquaporin-4 antibody levels in neuromye-
litis optica attacks Ann Neurol 201476305ndash309
17 Steinman L The re-emergence of antigen-specific toler-
ance as a potential therapy for MS Mult Scler 201521
1223ndash1238
18 Roep BO Solvason N Gottlieb PA et al Plasmid
encoded proinsulin preserves C-peptide while specifically
reducing proinsulin specific CD8 T cells in type 1 diabe-
tes Sci Transl Med 20135191ra82
19 Feldmann M Steinman L Design of effective immuno-
therapy for human autoimmunity Nature 2005435
612ndash619
20 Garren H Robinson WH Krasulovaacute E et al Phase 2 trial
of a DNA vaccine encoding myelin basic protein for mul-
tiple sclerosis Ann Neurol 200863611ndash620
21 Bar-Or A Vollmer T Antel J et al Induction of antigen-
specific tolerance in multiple sclerosis after immunization
with DNA encoding myelin basic protein in a randomized
placebo-controlled phase 12 trial Arch Neurol 200764
1407ndash1415
22 Selmi C Autoimmunity in 2012 Clin Rev Allergy
Immunol 201345290ndash301
23 Martin A Tisch RM Getts DR Manipulating T cell-
mediated pathology targets and functions of monoclonal
antibody immunotherapy Clin Immunol 2013148
136ndash147
24 Salvana EM Salata RA Infectious complications associ-
ated with monoclonal antibodies and related small mole-
cules Clin Microbiol Rev 200922274ndash290
25 Madakamutil LT Maricic I Sercarz EE Kumar V
Immunodominance in the TCR repertoire of a TCR
peptide-specific CD41 Treg population that controls
experimental autoimmune encephalomyelitis J Immunol
20081804577ndash4585
26 Buckner JH Mechanisms of impaired regulation by CD4
(1)CD25(1)FOXP3(1) regulatory T cells in human
autoimmune diseases Nat Rev Immunol 201010849ndash
859
27 Linhares UC Schiavoni PB Barros PO et al The ex vivo
production of IL-6 and IL-21 by CD41 T cells is directly
associated with neurological disability in neuromyelitis op-
tica patients J Clin Immunol 201333179ndash189
28 Pohl M Fisher MT Mader S et al Pathogenic T cells
responses against aquaporin 4 Acta Neuropathol 2011
12221ndash34
29 Varrin-Doyer M Spencer CM Schulze-Topphoff U et al
Aquaporin 4-specific T cells in neuromyelitis optica
exhibit a Th17 bias and recognize clostridium ABC trans-
porter Ann Neurol 20127253ndash64
30 Kumar V Sercarz EE The involvement of T cell receptor
peptide-specific regulatory CD41 T cells in recovery from
antigen-induced autoimmune disease J Exp Med 1993
178909ndash916
31 Zhang J T cell vaccination as an immunotherapy for
autoimmune diseases Cell Mol Immunol 20041321ndash
327
32 Huang X Wu H Lu Q The mechanisms and applica-
tions of T cell vaccination for autoimmune diseases a com-
prehensive review Clin Rev Allergy Immunol 201447
219ndash233
33 Fontana R Bregni M Cipponi A et al Peripheral blood
lymphocytes genetically modified to express the selftumor
antigen MAGE-A3 induce antitumor immune responses
in cancer patients Blood 20091131651ndash1660
34 Fox E Wynn D Cohan S Rill D McGuire D
Markowitz C A randomized clinical trial of autolo-
gous T-cell therapy in multiple sclerosis subset anal-
ysis and implications for trial design Mult Scler 2012
18843ndash852
35 Chen G Li N Zang YC et al Vaccination with selected
synovial T cells in rheumatoid arthritis Arthritis Rheum
200756453ndash463
36 Li ZG Mu R Dai ZP Gao XM T cell vaccination in
systemic lupus erythematosus with autologous activated T
cells Lupus 200514884ndash889
37 Leavy O Dendritic cells tracing the origins of cDCs Nat
Rev Immunol 201313703
38 Banchereau J Palucka AK Dendritic cells as therapeutic
vaccines against cancer Nat Rev Immunol 20055296ndash
306
39 Vacchelli E Vitale I Eggermont A et al Trial watch
dendritic cell-based interventions for cancer therapy
Oncoimmunology 201211111ndash1134
40 Mantia-Smaldone GM Chu CS A review of dendritic cell
therapy for cancer progress and challenges BioDrugs
201327453ndash468
41 Segovia-Gamboa N Rodriguez-Arellano ME Rangel-
Cruz R et al Tolerogenic dendritic cells induce antigen-
specific hyporesponsiveness in insulin- and glutamic acid
decarboxylase 65-autoreactive T lymphocytes from type 1
diabetic patients Clin Immunol 201415472ndash83
42 Giannoukakis N Phillips B Finegold D Harnaha J
Trucco M Phase I (safety) study of autologous tolerogenic
dendritic cells in type 1 diabetic patients Diabetes Care
2011342026ndash2032
43 Boks MA Kager-Groenland J Haasjes MSP Zwaginga JJ
van Ham SM ten Brinke A IL-10-generated tolerogenic
dendritic cells are optimal for functional regulatory T cell
induction- a comparative study on human clinical-
applicable DC Clin Immunol 2012142332ndash342
44 Saito M Nagasaw M Takada H et al Defective IL-10
signaling in hyper-IgE syndrome results in impaired gen-
eration of tolerogenic dendritic cells and induced regula-
tory T cells J Exp Med 2011208235ndash249
45 Miller SD Turley DM Podojil JR Antigen-specific tol-
erance strategies for the prevention and treatment of auto-
immune disease Nat Rev Immunol 20077665ndash677
46 Karpus WJ Pope JG Peterson JD Dal Canto MC
Miller SD Inhibition of Theilerrsquos virus-mediated demye-
lination by peripheral immune tolerance induction
J Immunol 1995155947ndash957
47 Kennedy KJ Smith WS Miller SD Karpus WJ Induc-
tion of antigen-specific tolerance for the treatment of
ongoing relapsing autoimmune encephalomyelitis a com-
parison between oral and peripheral tolerance J Immunol
19971591036ndash1044
48 Kennedy MK Tan LJ Dal Canto MC et al Inhibition of
murine relapsing experimental autoimmune encephalomy-
elitis by immune tolerance to proteolipid protein and its
encephalitogenic peptides J Immunol 1990144909ndash
915
49 Turley DM Miller SD Peripheral tolerance induction
using ethylenecarbodiimide-fixed APCs uses both direct
and indirect mechanisms of antigen presentation for
Neurology Neuroimmunology amp Neuroinflammation 9
ordf 2016 American Academy of Neurology Unauthorized reproduction of this article is prohibited
prevention of experimental autoimmune encephalomyeli-
tis J Immunol 20071782212ndash2220
50 Getts DR Turley DM Smith CE et al Tolerance
induced by apoptotic antigen-coupled leukocytes is
induced by PD-L11 and IL-10-producing splenic macro-
phages and maintained by T regulatory cells J Immunol
20111872405ndash2417
51 Luo X Pothoven KL McCarthy D et al ECDI-fixed allo-
geneic splenocytes induce donor-specific tolerance for long-
term survival of islet transplants via two distinct mechanisms
Proc Natl Acad Sci USA 200810514527ndash14532
52 Lutterotti A Yousef S Sputtek A et al Antigen-specific
tolerance by autologous myelin peptide-coupled cells
a phase 1 trial in multiple sclerosis Sci Transl Med
20135188ra75
53 Bielekova B SungMH KadomN Simon R McFarland HF
Martin R Expansion and functional relevance of high-avidity
myelin-specific CD41 T cells in multiple sclerosis
J Immunol 20041723893ndash3904
54 Jenkins MK Mueller D Schwartz RH et al Induction
and maintenance of anergy in mature T cells Adv Exp
Med Biol 1991292167ndash176
55 Peschl P Reindl M Schanda K Sospedra M
Martin R Lutterotti A Anti-myelin antibody re-
sponses following induction of antigen-specific toler-
ance with antigen-coupled cells J Neuroimmunol
201427512
56 Tuohy VK Yu M Yin L Kawczak JA Kinkel PR Regres-
sion and spreading of self-recognition during the develop-
ment of autoimmune demyelinating disease J Autoimmun
19991311ndash20
57 Vanderlugt CL Miller SD Epitope spreading in immune-
mediated diseases implications for immunotherapy Nat
Rev Immunol 2002285ndash95
58 Stromnes IM Schmitt TM Chapuis AG Hingorani SR
Greenberg PD Re-adapting T cells for cancer therapy
from mouse models to clinical trials Immunol Rev
2014257145ndash164
10 Neurology Neuroimmunology amp Neuroinflammation
ordf 2016 American Academy of Neurology Unauthorized reproduction of this article is prohibited
DOI 101212NXI000000000000027620163 Neurol Neuroimmunol Neuroinflamm
Larry Steinman Amit Bar-Or Jacinta M Behne et al Restoring immune tolerance in neuromyelitis optica Part I
This information is current as of September 7 2016
2016 American Academy of Neurology All rights reserved Online ISSN 2332-7812Published since April 2014 it is an open-access online-only continuous publication journal Copyright copy
is an official journal of the American Academy of NeurologyNeurol Neuroimmunol Neuroinflamm
ServicesUpdated Information amp
httpnnneurologyorgcontent35e276fullhtmlincluding high resolution figures can be found at
Supplementary Material
httpnnneurologyorgcontentsuppl2016090735e276DC2 httpnnneurologyorgcontentsuppl2016090735e276DC1
Supplementary material can be found at
References httpnnneurologyorgcontent35e276fullhtmlref-list-1
This article cites 58 articles 18 of which you can access for free at
Subspecialty Collections
httpnnneurologyorgcgicollectiontransverse_myelitisTransverse myelitis
httpnnneurologyorgcgicollectionoptic_neuritisOptic neuritis see Neuro-ophthalmologyOptic Nerve
httpnnneurologyorgcgicollectiondevics_syndromeDevics syndrome
httpnnneurologyorgcgicollectionautoimmune_diseasesAutoimmune diseasesfollowing collection(s) This article along with others on similar topics appears in the
Permissions amp Licensing
httpnnneurologyorgmiscaboutxhtmlpermissionsits entirety can be found online atInformation about reproducing this article in parts (figurestables) or in
Reprints
httpnnneurologyorgmiscaddirxhtmlreprintsusInformation about ordering reprints can be found online
2016 American Academy of Neurology All rights reserved Online ISSN 2332-7812Published since April 2014 it is an open-access online-only continuous publication journal Copyright copy
is an official journal of the American Academy of NeurologyNeurol Neuroimmunol Neuroinflamm
affinity TcR against a class II MHC fused to IL-10has been developed as a means for downregulatingautoimmune responsese6 Optimizing the tissue tar-geting of these constructs remains challenging
Adoptive T cell transfer of enriched Tregs has alsobeen used as a method of antigen-specific toleriza-tione7e8 Yet optimal delivery of antigen-specific sta-ble Tregs remains to be determined Moreover TcRaffinity and specificity of Tregse9 is less well charac-terized than those of effector T cells Several studieshave demonstrated that introducing class IndashrestrictedTcR into Tregs can redirect their specific activitiese10
and that TcRs with KDs of approximately 1 mM maybe of sufficient avidity to drive Treg activitye11 TcRsdirected against class I targets required lower affinitiesthan those in effector CD41 T cells that promoteTreg activity
Opportunities to generate mouse models and ther-apeutic T cells targeting AQP4 would appear to benumerouse12 For example in a mouse model animmunogenic response can be elicited with effectorT cells (CD41 andor CD81) expressing an AQP4-specific antibody (scFv)e13 fused to a chimeric antigenreceptor (CAR) Redirection of Tregs to suppresseffector cells could provide greater therapeutic poten-tial and utilize CARs directed against AQP4 Alter-natively conventional TcRs targeting AQP4 peptideMHC class I complexes such as human leukocyteantigen (HLA)-A2 (which are expressed by 40 ofthe population) might prove effective Possible advan-tages of CARs include their potential use of scFv fromexisting monoclonal antibodiese13 Furthermore thesame scFv used in effector T cells to initiate patho-genic responses could instead be engineered to a Tregphenotype Here scFv affinity could be optimizedusing single-site complementarity determining re-gions mutations Treg signaling and activity woulddepend on the CAR signaling domain specificityand affinity as well as density of AQP4 displayedon target cells Animal models that more preciselyrecapitulate human disease would represent signifi-cant advances toward these goals
The TcR approach offers potential advantage overscFvCAR including direct targeting of Treg activityto class Indashbearing tissues and regional lymph nodeswhere AQP4 might be cross-presented by MHCOstensibly this strategy might arrest or reversecross-presentation of AQP4 antigens by class IIMHC which may occur early in the developmentof NMOSDe14 If so this approach may also mitigateepitope spreading The generation of TcRs againstAQP4 peptides that bind to HLA-A2 would be rela-tively straightforward given the extensive knowledgeof its binding motifs and the likelihood that AQP4contains cognate epitopese15 The TcR affinity in thisscenario may prove inadequate but such limitations
might be overcome through the generation of CARmutants
AQP4 antigenndashspecific approaches must addressissues well beyond TcR and CAR affinity Theseinclude untoward conversion of Tregs to pathogenicphenotypes such as those exhibiting either Th1 orTh17 paradigmse16 accurate tissue targeting and pro-motion of memory and stabilitye17e18 It is possiblethat the same tolerance checkpoints currently beingconsidered for rapid deletion of undesirable T cellstransferred in cancer could be applied to NMOSD
SUMMARY AND PROSPECTUS PART I Immunetolerization attempts to restore central or peripheral tol-erance thereby suppressing autoimmune diseases Thereis reason for optimism that this approach might offersubstantial clinical benefit to individuals with NMOSD DNA vaccination T cell vaccination tolerogenicDCs and peptide-coupling strategies have advancedto clinic trials Other promising approaches (egCAR-based or Treg-based therapeutics) are in earlierdevelopmental stages in autoimmune diseases Yetdefining target cell lineages to yield durable efficacyand determining stage of disease development optimalfor intervention remain crucial ongoing challenges Pastfailures using similar strategies have provided criticalinsights that despite the complexities of existing andemerging technologies for promoting tolerance shouldilluminate the pathway forward If so such advances intreatment could benefit individuals with NMOSDand other autoimmune diseases including arrestingdisease progression prolonging intervals to remissionenabling tissue repair and ultimately avoiding chronicimmunosuppressive therapy
AUTHOR CONTRIBUTIONSLarry Steinman draftingrevising the manuscript Amit Bar-Or drafting
revising the manuscript Jacinta M Behne revising the manuscript
Daniel Benitez-Ribas draftingrevising the manuscript Peter S Chin
draftingrevising the manuscript Michael Clare-Salzler draftingrevising
the manuscript Donald Healey draftingrevising the manuscript James
I Kim draftingrevising the manuscript David M Kranz draftingrevis-
ing the manuscript Andreas Lutterotti draftingrevising the manuscript
Roland Martin draftingrevising the manuscript Sven Schippling draft-
ingrevising the manuscript Pablo Villoslada draftingrevising the man-
uscript Cheng-Hong Wei draftingrevising the manuscript Howard L
Weiner draftingrevising the manuscript Scott S Zamvil draftingrevis-
ing the manuscript Michael R Yeaman draftingrevising the manu-
script study concept or design analysis or interpretation of data
accepts responsibility for conduct of research and will give final approval
Terry J Smith draftingrevising the manuscript study concept or design
analysis or interpretation of data accepts responsibility for conduct of
research and will give final approval
ACKNOWLEDGMENTWe thank Dr Gerald Nepom Dr Philip Bernstein and Dr William St
Clair for their helpful comments
STUDY FUNDINGThis work was supported in part by the Guthy-Jackson Charitable
Foundation
Neurology Neuroimmunology amp Neuroinflammation 7
ordf 2016 American Academy of Neurology Unauthorized reproduction of this article is prohibited
DISCLOSUREL Steinman served on the scientific advisory board for Novartis Recep-
tos Atreca Tolerion Teva received travel funding andor speaker hon-
oraria from Biogen Bayhill Bayer Celgene Receptos is on the editorial
board for Multiple Sclerosis Journal Proceedings of the National Academy of
Science holds a patents for antigen-specific tolerance has a patent pend-
ing for cytokines and type 1 interferons is on the speakers bureau for
EMD Serono received research support from NIH has stock options
and board membership in Tolerion is on the board of directors for
BioAtla A Bar-Or is on the scientific advisory board for Dionix
Receptos-Celgene RocheGenentech Novartis GSK Guthy-Jackson
Greater Good Foundation Immune Tolerance Network received travel
funding andor speaker honoraria from Receptos-Celgene RocheGen-
entech Novartis Sanofi-Genzyme GSK served on the editorial board
for Neurologyreg Clinical and Experimental Neuroimmunology consulted
for DioGenix Receptos-Celgene RocheGenentech Novartis Sanofi-
Genzyme GSK received research support from Novartis Sanofi-
Genzyme JM Behne and D Benitez-Ribas report no disclosures PS
Chin served as the medical director for Genentech and Novartis Pharma-
ceuticals Corp M Clare-Salzler received research support from NIH
NIAID D Healy has a patent pending for the treatment of B cellndash
mediated autoimmunities with T cell vaccination has been CSO for
Opexa Therapeutics received research support from and holds stock
stock options in Opexa Therapeutics JI Kim is an associate editor for
the Journal of Negative Results in Biomedicine is employed by Unum
Therapeutics DM Kranz has patents and patents pending in the areas
of yeast display and T cell receptor engineering and receives license fee
payments from them consulted for AbbVie received research support
from NIH Melanoma Research Alliance A Lutterotti served on the
scientific advisory board for Bayer Biogen Novartis Genzyme received
travel support from European Charcot Foundation Fundacio GAEM
holds a patent with the University of Zurich received research support
from Wyss Translational Center Austrian MS Society R Martin served
on the scientific advisory board for Biogen Merck Serono Teva
Genzyme Sanofi-Aventis CellProtect Neuway received speaker hono-
raria from Biogen Merck Serono Novartis Roche Genzyme holds
a patent for the therapeutic efficacy of anto-CD25 monoclonal antibody
treatment in combination with IFN-b in MS consulted for Myelin
Repair Foundation The Weatherall Institute of Molecular Studies Uni-
versity of Oxford the Hertie Foundation is a member of the Kuratorium
of the Jung Foundation for Science received research support from
Novartis Biogen Swiss National Science Foundation European Union
Seventh Framework Program European Research Council S Schippling
served on the scientific advisory board for Bayer Healthcare Biogen
Merck Serono Novartis Sanofi-Genzyme TEVA received travel fund-
ing andor speaker honoraria from Bayer Healthcare Biogen Merck
Serono Novartis Sanofi-Aventis TEVA is an associate editor for Fron-
tiers in Neurology holds a patent for therapeutic vaccination in PML
using VP1 and Il7 received research support from Sanofi-Genzyme
Novartis University of Zurich Betty and David Koetser Foundation
for Brain Research Swiss Multiple Sclerosis Society P Villoslada received
travel funding andor speaker honoraria from Novartis Roche Genzyme
served as an academic editor for PLoS One served on the editorial board
for Neurology and Therapy Current Treatment Options in Neurology Mul-
tiple Sclerosis and Demyelinating Disorders holds a patent for methylthioa-
denosine for the treatment of MS Agnostic neurotrophic compounds for
the treatment of brain diseases Gene signature pattern as a biomarker for
MS Algorithm for quantifying fractal dimension in brain MRI received
research support from Novartis Roche Genzyme Instituto de Salud
Carlos III European Commission National MS Society Fundacion
Maraton TV3 holds stock or stock options in Bionure Inc Spire Bio-
ventures Mint-Labs C-H Wei reports no disclosures HL Weiner
served on the scientific advisory board for the Guthy-Jackson Charitable
Foundation Teva Pharmaceutical Industries Biogen Idec Novartis
Sanofi-Aventis consulted for Therapix Biogen Novartis Serono Teva
Sanofi received research support from National Multiple Sclerosis Soci-
ety S Zamvil served on the scientific advisory board for BioMS Teva
Pharmaceuticals Eli Lilly and Co Myelin Repair Foundation is deputy
editor for Neurologyreg Neuroimmunology amp Neuroinflammation consulted
for Biogen Idec Teva Neuroscience EMD Serono Genzyme Novartis
Roche is on the speakers bureau for Advanced Health Media Biogen
received research support from NIH NMSS Alexander M and June L
Maisin Foundation MR Yeaman is on the scientific advisory board for
Guthy-Jackson Charitable Foundation served as an associate editor for
PLoS Pathogens holds patents for Vaccines targeting drug-resistant patho-
gens immunotherapies targeting drug-resistant pathogens novel anti-
infective biological therapeutics novel anti-infective small molecules
novel biological regulating programmed cell death consulted for
Guthy-Jackson Charitable Foundation received research support from
NovaDigm Therapeutics Inc Metacin Inc US Department of Defense
NIH holds stock or stock options for NovaDigm Therapeutics Inc Meta-
cin Inc receives license fee and royalty payments from NovaDigm Thera-
peutics TJ Smith received research support from NIH University of South
Denmark Bell Charitable Foundation RPB Foundation is a member of the
Guthy-Jackson Charitable Foundation scientific advisory board and holds
patents covering the blockade of insulin-like growth factor receptor-l in
autoimmune diseases Go to Neurologyorgnn for full disclosure forms
Received February 12 2016 Accepted in final form July 15 2016
REFERENCES1 Levy M Wildemann B Jarius S et al Immunopathogenesis
of neuromyelitis optica Adv Immunol 2014121213ndash242
2 Wingerchuk DM Banwell B Bennett JL et al Interna-
tional consensus diagnostic criteria for neuromyelitis opti-
ca spectrum disorders Neurology 201585177ndash189
3 Lennon VA Kryzer TJ Pittock SJ Verkman AS
Hinson SR IgG marker of optic-spinal multiple sclerosis
binds to the aquaporin-4 water channel J Exp Med 2005
202473ndash477
4 Elsone L Kitley J Luppe S et al Long-term efficacy toler-
ability and retention rate of azathioprine in 103 aquaporin-4
antibody-positive neuromyelitis optica spectrum disorder
patients a multicenter retrospective observational study from
the UK Mult Scler 2014201533ndash1540
5 Nakamura M Nakazawa T Doi H et al Early high-dose
intravenous methylprednisolone is effective in preserving
retinal nerve fiber layer thickness in patients with neuro-
myelitis optica Graefes Arch Clin Exp Ophthalmol 2010
2481777ndash1785
6 Miller SD Wetzig RP Claman HN The induction of
cell-mediated immunity and tolerance with protein anti-
gens coupled to syngeneic lymphoid cells J Exp Med
1979149758ndash773
7 Oh S Schwartz RH Singh NJ Development and toler-
ization of hyperacute rejection in a transgenic mouse graft
versus host model Transplantation 201294234ndash240
8 Castells M Rapid desensitization of hypersensitivity reactions
to chemotherapy agents Curr Drug Saf 20061243ndash251
9 Fousteri G Bresson D von Herrath M Rational develop-
ment of antigen-specific therapies for type 1 diabetes Adv
Exp Med Biol 2007601313ndash319
10 Turley DM Miller SD Prospects for antigen-specific tol-
erance based therapies for the treatment of multiple scle-
rosis Results Probl Cell Differ 201051217ndash235
11 Mori K Yamanishi H Ikeda Y et al Oral administration
of carbonic anhydrase I ameliorates murine experimental
colitis induced by Foxp3-CD41CD252 T cells J Leukoc
Biol 201393963ndash972
12 Genain CP Abel K Belmar N et al Late complications of
immune deviation therapy in a nonhuman primate Sci-
ence 19962742054ndash2057
13 Dolgin E The inverse of immunity Nat Med 201016
740ndash743
14 Steinman L Inverse vaccination the opposite of Jennerrsquos
concept for therapy of autoimmunity J Intern Med 2010
267441ndash451
8 Neurology Neuroimmunology amp Neuroinflammation
ordf 2016 American Academy of Neurology Unauthorized reproduction of this article is prohibited
15 Hinson SR McKeon A Lennon VA Neurological auto-
immunity targeting aquaporin-4 Neuroscience 2010168
1009ndash1018
16 Sato DK Callegaro D de Haidar Jorge FM et al Cere-
brospinal fluid aquaporin-4 antibody levels in neuromye-
litis optica attacks Ann Neurol 201476305ndash309
17 Steinman L The re-emergence of antigen-specific toler-
ance as a potential therapy for MS Mult Scler 201521
1223ndash1238
18 Roep BO Solvason N Gottlieb PA et al Plasmid
encoded proinsulin preserves C-peptide while specifically
reducing proinsulin specific CD8 T cells in type 1 diabe-
tes Sci Transl Med 20135191ra82
19 Feldmann M Steinman L Design of effective immuno-
therapy for human autoimmunity Nature 2005435
612ndash619
20 Garren H Robinson WH Krasulovaacute E et al Phase 2 trial
of a DNA vaccine encoding myelin basic protein for mul-
tiple sclerosis Ann Neurol 200863611ndash620
21 Bar-Or A Vollmer T Antel J et al Induction of antigen-
specific tolerance in multiple sclerosis after immunization
with DNA encoding myelin basic protein in a randomized
placebo-controlled phase 12 trial Arch Neurol 200764
1407ndash1415
22 Selmi C Autoimmunity in 2012 Clin Rev Allergy
Immunol 201345290ndash301
23 Martin A Tisch RM Getts DR Manipulating T cell-
mediated pathology targets and functions of monoclonal
antibody immunotherapy Clin Immunol 2013148
136ndash147
24 Salvana EM Salata RA Infectious complications associ-
ated with monoclonal antibodies and related small mole-
cules Clin Microbiol Rev 200922274ndash290
25 Madakamutil LT Maricic I Sercarz EE Kumar V
Immunodominance in the TCR repertoire of a TCR
peptide-specific CD41 Treg population that controls
experimental autoimmune encephalomyelitis J Immunol
20081804577ndash4585
26 Buckner JH Mechanisms of impaired regulation by CD4
(1)CD25(1)FOXP3(1) regulatory T cells in human
autoimmune diseases Nat Rev Immunol 201010849ndash
859
27 Linhares UC Schiavoni PB Barros PO et al The ex vivo
production of IL-6 and IL-21 by CD41 T cells is directly
associated with neurological disability in neuromyelitis op-
tica patients J Clin Immunol 201333179ndash189
28 Pohl M Fisher MT Mader S et al Pathogenic T cells
responses against aquaporin 4 Acta Neuropathol 2011
12221ndash34
29 Varrin-Doyer M Spencer CM Schulze-Topphoff U et al
Aquaporin 4-specific T cells in neuromyelitis optica
exhibit a Th17 bias and recognize clostridium ABC trans-
porter Ann Neurol 20127253ndash64
30 Kumar V Sercarz EE The involvement of T cell receptor
peptide-specific regulatory CD41 T cells in recovery from
antigen-induced autoimmune disease J Exp Med 1993
178909ndash916
31 Zhang J T cell vaccination as an immunotherapy for
autoimmune diseases Cell Mol Immunol 20041321ndash
327
32 Huang X Wu H Lu Q The mechanisms and applica-
tions of T cell vaccination for autoimmune diseases a com-
prehensive review Clin Rev Allergy Immunol 201447
219ndash233
33 Fontana R Bregni M Cipponi A et al Peripheral blood
lymphocytes genetically modified to express the selftumor
antigen MAGE-A3 induce antitumor immune responses
in cancer patients Blood 20091131651ndash1660
34 Fox E Wynn D Cohan S Rill D McGuire D
Markowitz C A randomized clinical trial of autolo-
gous T-cell therapy in multiple sclerosis subset anal-
ysis and implications for trial design Mult Scler 2012
18843ndash852
35 Chen G Li N Zang YC et al Vaccination with selected
synovial T cells in rheumatoid arthritis Arthritis Rheum
200756453ndash463
36 Li ZG Mu R Dai ZP Gao XM T cell vaccination in
systemic lupus erythematosus with autologous activated T
cells Lupus 200514884ndash889
37 Leavy O Dendritic cells tracing the origins of cDCs Nat
Rev Immunol 201313703
38 Banchereau J Palucka AK Dendritic cells as therapeutic
vaccines against cancer Nat Rev Immunol 20055296ndash
306
39 Vacchelli E Vitale I Eggermont A et al Trial watch
dendritic cell-based interventions for cancer therapy
Oncoimmunology 201211111ndash1134
40 Mantia-Smaldone GM Chu CS A review of dendritic cell
therapy for cancer progress and challenges BioDrugs
201327453ndash468
41 Segovia-Gamboa N Rodriguez-Arellano ME Rangel-
Cruz R et al Tolerogenic dendritic cells induce antigen-
specific hyporesponsiveness in insulin- and glutamic acid
decarboxylase 65-autoreactive T lymphocytes from type 1
diabetic patients Clin Immunol 201415472ndash83
42 Giannoukakis N Phillips B Finegold D Harnaha J
Trucco M Phase I (safety) study of autologous tolerogenic
dendritic cells in type 1 diabetic patients Diabetes Care
2011342026ndash2032
43 Boks MA Kager-Groenland J Haasjes MSP Zwaginga JJ
van Ham SM ten Brinke A IL-10-generated tolerogenic
dendritic cells are optimal for functional regulatory T cell
induction- a comparative study on human clinical-
applicable DC Clin Immunol 2012142332ndash342
44 Saito M Nagasaw M Takada H et al Defective IL-10
signaling in hyper-IgE syndrome results in impaired gen-
eration of tolerogenic dendritic cells and induced regula-
tory T cells J Exp Med 2011208235ndash249
45 Miller SD Turley DM Podojil JR Antigen-specific tol-
erance strategies for the prevention and treatment of auto-
immune disease Nat Rev Immunol 20077665ndash677
46 Karpus WJ Pope JG Peterson JD Dal Canto MC
Miller SD Inhibition of Theilerrsquos virus-mediated demye-
lination by peripheral immune tolerance induction
J Immunol 1995155947ndash957
47 Kennedy KJ Smith WS Miller SD Karpus WJ Induc-
tion of antigen-specific tolerance for the treatment of
ongoing relapsing autoimmune encephalomyelitis a com-
parison between oral and peripheral tolerance J Immunol
19971591036ndash1044
48 Kennedy MK Tan LJ Dal Canto MC et al Inhibition of
murine relapsing experimental autoimmune encephalomy-
elitis by immune tolerance to proteolipid protein and its
encephalitogenic peptides J Immunol 1990144909ndash
915
49 Turley DM Miller SD Peripheral tolerance induction
using ethylenecarbodiimide-fixed APCs uses both direct
and indirect mechanisms of antigen presentation for
Neurology Neuroimmunology amp Neuroinflammation 9
ordf 2016 American Academy of Neurology Unauthorized reproduction of this article is prohibited
prevention of experimental autoimmune encephalomyeli-
tis J Immunol 20071782212ndash2220
50 Getts DR Turley DM Smith CE et al Tolerance
induced by apoptotic antigen-coupled leukocytes is
induced by PD-L11 and IL-10-producing splenic macro-
phages and maintained by T regulatory cells J Immunol
20111872405ndash2417
51 Luo X Pothoven KL McCarthy D et al ECDI-fixed allo-
geneic splenocytes induce donor-specific tolerance for long-
term survival of islet transplants via two distinct mechanisms
Proc Natl Acad Sci USA 200810514527ndash14532
52 Lutterotti A Yousef S Sputtek A et al Antigen-specific
tolerance by autologous myelin peptide-coupled cells
a phase 1 trial in multiple sclerosis Sci Transl Med
20135188ra75
53 Bielekova B SungMH KadomN Simon R McFarland HF
Martin R Expansion and functional relevance of high-avidity
myelin-specific CD41 T cells in multiple sclerosis
J Immunol 20041723893ndash3904
54 Jenkins MK Mueller D Schwartz RH et al Induction
and maintenance of anergy in mature T cells Adv Exp
Med Biol 1991292167ndash176
55 Peschl P Reindl M Schanda K Sospedra M
Martin R Lutterotti A Anti-myelin antibody re-
sponses following induction of antigen-specific toler-
ance with antigen-coupled cells J Neuroimmunol
201427512
56 Tuohy VK Yu M Yin L Kawczak JA Kinkel PR Regres-
sion and spreading of self-recognition during the develop-
ment of autoimmune demyelinating disease J Autoimmun
19991311ndash20
57 Vanderlugt CL Miller SD Epitope spreading in immune-
mediated diseases implications for immunotherapy Nat
Rev Immunol 2002285ndash95
58 Stromnes IM Schmitt TM Chapuis AG Hingorani SR
Greenberg PD Re-adapting T cells for cancer therapy
from mouse models to clinical trials Immunol Rev
2014257145ndash164
10 Neurology Neuroimmunology amp Neuroinflammation
ordf 2016 American Academy of Neurology Unauthorized reproduction of this article is prohibited
DOI 101212NXI000000000000027620163 Neurol Neuroimmunol Neuroinflamm
Larry Steinman Amit Bar-Or Jacinta M Behne et al Restoring immune tolerance in neuromyelitis optica Part I
This information is current as of September 7 2016
2016 American Academy of Neurology All rights reserved Online ISSN 2332-7812Published since April 2014 it is an open-access online-only continuous publication journal Copyright copy
is an official journal of the American Academy of NeurologyNeurol Neuroimmunol Neuroinflamm
ServicesUpdated Information amp
httpnnneurologyorgcontent35e276fullhtmlincluding high resolution figures can be found at
Supplementary Material
httpnnneurologyorgcontentsuppl2016090735e276DC2 httpnnneurologyorgcontentsuppl2016090735e276DC1
Supplementary material can be found at
References httpnnneurologyorgcontent35e276fullhtmlref-list-1
This article cites 58 articles 18 of which you can access for free at
Subspecialty Collections
httpnnneurologyorgcgicollectiontransverse_myelitisTransverse myelitis
httpnnneurologyorgcgicollectionoptic_neuritisOptic neuritis see Neuro-ophthalmologyOptic Nerve
httpnnneurologyorgcgicollectiondevics_syndromeDevics syndrome
httpnnneurologyorgcgicollectionautoimmune_diseasesAutoimmune diseasesfollowing collection(s) This article along with others on similar topics appears in the
Permissions amp Licensing
httpnnneurologyorgmiscaboutxhtmlpermissionsits entirety can be found online atInformation about reproducing this article in parts (figurestables) or in
Reprints
httpnnneurologyorgmiscaddirxhtmlreprintsusInformation about ordering reprints can be found online
2016 American Academy of Neurology All rights reserved Online ISSN 2332-7812Published since April 2014 it is an open-access online-only continuous publication journal Copyright copy
is an official journal of the American Academy of NeurologyNeurol Neuroimmunol Neuroinflamm
DISCLOSUREL Steinman served on the scientific advisory board for Novartis Recep-
tos Atreca Tolerion Teva received travel funding andor speaker hon-
oraria from Biogen Bayhill Bayer Celgene Receptos is on the editorial
board for Multiple Sclerosis Journal Proceedings of the National Academy of
Science holds a patents for antigen-specific tolerance has a patent pend-
ing for cytokines and type 1 interferons is on the speakers bureau for
EMD Serono received research support from NIH has stock options
and board membership in Tolerion is on the board of directors for
BioAtla A Bar-Or is on the scientific advisory board for Dionix
Receptos-Celgene RocheGenentech Novartis GSK Guthy-Jackson
Greater Good Foundation Immune Tolerance Network received travel
funding andor speaker honoraria from Receptos-Celgene RocheGen-
entech Novartis Sanofi-Genzyme GSK served on the editorial board
for Neurologyreg Clinical and Experimental Neuroimmunology consulted
for DioGenix Receptos-Celgene RocheGenentech Novartis Sanofi-
Genzyme GSK received research support from Novartis Sanofi-
Genzyme JM Behne and D Benitez-Ribas report no disclosures PS
Chin served as the medical director for Genentech and Novartis Pharma-
ceuticals Corp M Clare-Salzler received research support from NIH
NIAID D Healy has a patent pending for the treatment of B cellndash
mediated autoimmunities with T cell vaccination has been CSO for
Opexa Therapeutics received research support from and holds stock
stock options in Opexa Therapeutics JI Kim is an associate editor for
the Journal of Negative Results in Biomedicine is employed by Unum
Therapeutics DM Kranz has patents and patents pending in the areas
of yeast display and T cell receptor engineering and receives license fee
payments from them consulted for AbbVie received research support
from NIH Melanoma Research Alliance A Lutterotti served on the
scientific advisory board for Bayer Biogen Novartis Genzyme received
travel support from European Charcot Foundation Fundacio GAEM
holds a patent with the University of Zurich received research support
from Wyss Translational Center Austrian MS Society R Martin served
on the scientific advisory board for Biogen Merck Serono Teva
Genzyme Sanofi-Aventis CellProtect Neuway received speaker hono-
raria from Biogen Merck Serono Novartis Roche Genzyme holds
a patent for the therapeutic efficacy of anto-CD25 monoclonal antibody
treatment in combination with IFN-b in MS consulted for Myelin
Repair Foundation The Weatherall Institute of Molecular Studies Uni-
versity of Oxford the Hertie Foundation is a member of the Kuratorium
of the Jung Foundation for Science received research support from
Novartis Biogen Swiss National Science Foundation European Union
Seventh Framework Program European Research Council S Schippling
served on the scientific advisory board for Bayer Healthcare Biogen
Merck Serono Novartis Sanofi-Genzyme TEVA received travel fund-
ing andor speaker honoraria from Bayer Healthcare Biogen Merck
Serono Novartis Sanofi-Aventis TEVA is an associate editor for Fron-
tiers in Neurology holds a patent for therapeutic vaccination in PML
using VP1 and Il7 received research support from Sanofi-Genzyme
Novartis University of Zurich Betty and David Koetser Foundation
for Brain Research Swiss Multiple Sclerosis Society P Villoslada received
travel funding andor speaker honoraria from Novartis Roche Genzyme
served as an academic editor for PLoS One served on the editorial board
for Neurology and Therapy Current Treatment Options in Neurology Mul-
tiple Sclerosis and Demyelinating Disorders holds a patent for methylthioa-
denosine for the treatment of MS Agnostic neurotrophic compounds for
the treatment of brain diseases Gene signature pattern as a biomarker for
MS Algorithm for quantifying fractal dimension in brain MRI received
research support from Novartis Roche Genzyme Instituto de Salud
Carlos III European Commission National MS Society Fundacion
Maraton TV3 holds stock or stock options in Bionure Inc Spire Bio-
ventures Mint-Labs C-H Wei reports no disclosures HL Weiner
served on the scientific advisory board for the Guthy-Jackson Charitable
Foundation Teva Pharmaceutical Industries Biogen Idec Novartis
Sanofi-Aventis consulted for Therapix Biogen Novartis Serono Teva
Sanofi received research support from National Multiple Sclerosis Soci-
ety S Zamvil served on the scientific advisory board for BioMS Teva
Pharmaceuticals Eli Lilly and Co Myelin Repair Foundation is deputy
editor for Neurologyreg Neuroimmunology amp Neuroinflammation consulted
for Biogen Idec Teva Neuroscience EMD Serono Genzyme Novartis
Roche is on the speakers bureau for Advanced Health Media Biogen
received research support from NIH NMSS Alexander M and June L
Maisin Foundation MR Yeaman is on the scientific advisory board for
Guthy-Jackson Charitable Foundation served as an associate editor for
PLoS Pathogens holds patents for Vaccines targeting drug-resistant patho-
gens immunotherapies targeting drug-resistant pathogens novel anti-
infective biological therapeutics novel anti-infective small molecules
novel biological regulating programmed cell death consulted for
Guthy-Jackson Charitable Foundation received research support from
NovaDigm Therapeutics Inc Metacin Inc US Department of Defense
NIH holds stock or stock options for NovaDigm Therapeutics Inc Meta-
cin Inc receives license fee and royalty payments from NovaDigm Thera-
peutics TJ Smith received research support from NIH University of South
Denmark Bell Charitable Foundation RPB Foundation is a member of the
Guthy-Jackson Charitable Foundation scientific advisory board and holds
patents covering the blockade of insulin-like growth factor receptor-l in
autoimmune diseases Go to Neurologyorgnn for full disclosure forms
Received February 12 2016 Accepted in final form July 15 2016
REFERENCES1 Levy M Wildemann B Jarius S et al Immunopathogenesis
of neuromyelitis optica Adv Immunol 2014121213ndash242
2 Wingerchuk DM Banwell B Bennett JL et al Interna-
tional consensus diagnostic criteria for neuromyelitis opti-
ca spectrum disorders Neurology 201585177ndash189
3 Lennon VA Kryzer TJ Pittock SJ Verkman AS
Hinson SR IgG marker of optic-spinal multiple sclerosis
binds to the aquaporin-4 water channel J Exp Med 2005
202473ndash477
4 Elsone L Kitley J Luppe S et al Long-term efficacy toler-
ability and retention rate of azathioprine in 103 aquaporin-4
antibody-positive neuromyelitis optica spectrum disorder
patients a multicenter retrospective observational study from
the UK Mult Scler 2014201533ndash1540
5 Nakamura M Nakazawa T Doi H et al Early high-dose
intravenous methylprednisolone is effective in preserving
retinal nerve fiber layer thickness in patients with neuro-
myelitis optica Graefes Arch Clin Exp Ophthalmol 2010
2481777ndash1785
6 Miller SD Wetzig RP Claman HN The induction of
cell-mediated immunity and tolerance with protein anti-
gens coupled to syngeneic lymphoid cells J Exp Med
1979149758ndash773
7 Oh S Schwartz RH Singh NJ Development and toler-
ization of hyperacute rejection in a transgenic mouse graft
versus host model Transplantation 201294234ndash240
8 Castells M Rapid desensitization of hypersensitivity reactions
to chemotherapy agents Curr Drug Saf 20061243ndash251
9 Fousteri G Bresson D von Herrath M Rational develop-
ment of antigen-specific therapies for type 1 diabetes Adv
Exp Med Biol 2007601313ndash319
10 Turley DM Miller SD Prospects for antigen-specific tol-
erance based therapies for the treatment of multiple scle-
rosis Results Probl Cell Differ 201051217ndash235
11 Mori K Yamanishi H Ikeda Y et al Oral administration
of carbonic anhydrase I ameliorates murine experimental
colitis induced by Foxp3-CD41CD252 T cells J Leukoc
Biol 201393963ndash972
12 Genain CP Abel K Belmar N et al Late complications of
immune deviation therapy in a nonhuman primate Sci-
ence 19962742054ndash2057
13 Dolgin E The inverse of immunity Nat Med 201016
740ndash743
14 Steinman L Inverse vaccination the opposite of Jennerrsquos
concept for therapy of autoimmunity J Intern Med 2010
267441ndash451
8 Neurology Neuroimmunology amp Neuroinflammation
ordf 2016 American Academy of Neurology Unauthorized reproduction of this article is prohibited
15 Hinson SR McKeon A Lennon VA Neurological auto-
immunity targeting aquaporin-4 Neuroscience 2010168
1009ndash1018
16 Sato DK Callegaro D de Haidar Jorge FM et al Cere-
brospinal fluid aquaporin-4 antibody levels in neuromye-
litis optica attacks Ann Neurol 201476305ndash309
17 Steinman L The re-emergence of antigen-specific toler-
ance as a potential therapy for MS Mult Scler 201521
1223ndash1238
18 Roep BO Solvason N Gottlieb PA et al Plasmid
encoded proinsulin preserves C-peptide while specifically
reducing proinsulin specific CD8 T cells in type 1 diabe-
tes Sci Transl Med 20135191ra82
19 Feldmann M Steinman L Design of effective immuno-
therapy for human autoimmunity Nature 2005435
612ndash619
20 Garren H Robinson WH Krasulovaacute E et al Phase 2 trial
of a DNA vaccine encoding myelin basic protein for mul-
tiple sclerosis Ann Neurol 200863611ndash620
21 Bar-Or A Vollmer T Antel J et al Induction of antigen-
specific tolerance in multiple sclerosis after immunization
with DNA encoding myelin basic protein in a randomized
placebo-controlled phase 12 trial Arch Neurol 200764
1407ndash1415
22 Selmi C Autoimmunity in 2012 Clin Rev Allergy
Immunol 201345290ndash301
23 Martin A Tisch RM Getts DR Manipulating T cell-
mediated pathology targets and functions of monoclonal
antibody immunotherapy Clin Immunol 2013148
136ndash147
24 Salvana EM Salata RA Infectious complications associ-
ated with monoclonal antibodies and related small mole-
cules Clin Microbiol Rev 200922274ndash290
25 Madakamutil LT Maricic I Sercarz EE Kumar V
Immunodominance in the TCR repertoire of a TCR
peptide-specific CD41 Treg population that controls
experimental autoimmune encephalomyelitis J Immunol
20081804577ndash4585
26 Buckner JH Mechanisms of impaired regulation by CD4
(1)CD25(1)FOXP3(1) regulatory T cells in human
autoimmune diseases Nat Rev Immunol 201010849ndash
859
27 Linhares UC Schiavoni PB Barros PO et al The ex vivo
production of IL-6 and IL-21 by CD41 T cells is directly
associated with neurological disability in neuromyelitis op-
tica patients J Clin Immunol 201333179ndash189
28 Pohl M Fisher MT Mader S et al Pathogenic T cells
responses against aquaporin 4 Acta Neuropathol 2011
12221ndash34
29 Varrin-Doyer M Spencer CM Schulze-Topphoff U et al
Aquaporin 4-specific T cells in neuromyelitis optica
exhibit a Th17 bias and recognize clostridium ABC trans-
porter Ann Neurol 20127253ndash64
30 Kumar V Sercarz EE The involvement of T cell receptor
peptide-specific regulatory CD41 T cells in recovery from
antigen-induced autoimmune disease J Exp Med 1993
178909ndash916
31 Zhang J T cell vaccination as an immunotherapy for
autoimmune diseases Cell Mol Immunol 20041321ndash
327
32 Huang X Wu H Lu Q The mechanisms and applica-
tions of T cell vaccination for autoimmune diseases a com-
prehensive review Clin Rev Allergy Immunol 201447
219ndash233
33 Fontana R Bregni M Cipponi A et al Peripheral blood
lymphocytes genetically modified to express the selftumor
antigen MAGE-A3 induce antitumor immune responses
in cancer patients Blood 20091131651ndash1660
34 Fox E Wynn D Cohan S Rill D McGuire D
Markowitz C A randomized clinical trial of autolo-
gous T-cell therapy in multiple sclerosis subset anal-
ysis and implications for trial design Mult Scler 2012
18843ndash852
35 Chen G Li N Zang YC et al Vaccination with selected
synovial T cells in rheumatoid arthritis Arthritis Rheum
200756453ndash463
36 Li ZG Mu R Dai ZP Gao XM T cell vaccination in
systemic lupus erythematosus with autologous activated T
cells Lupus 200514884ndash889
37 Leavy O Dendritic cells tracing the origins of cDCs Nat
Rev Immunol 201313703
38 Banchereau J Palucka AK Dendritic cells as therapeutic
vaccines against cancer Nat Rev Immunol 20055296ndash
306
39 Vacchelli E Vitale I Eggermont A et al Trial watch
dendritic cell-based interventions for cancer therapy
Oncoimmunology 201211111ndash1134
40 Mantia-Smaldone GM Chu CS A review of dendritic cell
therapy for cancer progress and challenges BioDrugs
201327453ndash468
41 Segovia-Gamboa N Rodriguez-Arellano ME Rangel-
Cruz R et al Tolerogenic dendritic cells induce antigen-
specific hyporesponsiveness in insulin- and glutamic acid
decarboxylase 65-autoreactive T lymphocytes from type 1
diabetic patients Clin Immunol 201415472ndash83
42 Giannoukakis N Phillips B Finegold D Harnaha J
Trucco M Phase I (safety) study of autologous tolerogenic
dendritic cells in type 1 diabetic patients Diabetes Care
2011342026ndash2032
43 Boks MA Kager-Groenland J Haasjes MSP Zwaginga JJ
van Ham SM ten Brinke A IL-10-generated tolerogenic
dendritic cells are optimal for functional regulatory T cell
induction- a comparative study on human clinical-
applicable DC Clin Immunol 2012142332ndash342
44 Saito M Nagasaw M Takada H et al Defective IL-10
signaling in hyper-IgE syndrome results in impaired gen-
eration of tolerogenic dendritic cells and induced regula-
tory T cells J Exp Med 2011208235ndash249
45 Miller SD Turley DM Podojil JR Antigen-specific tol-
erance strategies for the prevention and treatment of auto-
immune disease Nat Rev Immunol 20077665ndash677
46 Karpus WJ Pope JG Peterson JD Dal Canto MC
Miller SD Inhibition of Theilerrsquos virus-mediated demye-
lination by peripheral immune tolerance induction
J Immunol 1995155947ndash957
47 Kennedy KJ Smith WS Miller SD Karpus WJ Induc-
tion of antigen-specific tolerance for the treatment of
ongoing relapsing autoimmune encephalomyelitis a com-
parison between oral and peripheral tolerance J Immunol
19971591036ndash1044
48 Kennedy MK Tan LJ Dal Canto MC et al Inhibition of
murine relapsing experimental autoimmune encephalomy-
elitis by immune tolerance to proteolipid protein and its
encephalitogenic peptides J Immunol 1990144909ndash
915
49 Turley DM Miller SD Peripheral tolerance induction
using ethylenecarbodiimide-fixed APCs uses both direct
and indirect mechanisms of antigen presentation for
Neurology Neuroimmunology amp Neuroinflammation 9
ordf 2016 American Academy of Neurology Unauthorized reproduction of this article is prohibited
prevention of experimental autoimmune encephalomyeli-
tis J Immunol 20071782212ndash2220
50 Getts DR Turley DM Smith CE et al Tolerance
induced by apoptotic antigen-coupled leukocytes is
induced by PD-L11 and IL-10-producing splenic macro-
phages and maintained by T regulatory cells J Immunol
20111872405ndash2417
51 Luo X Pothoven KL McCarthy D et al ECDI-fixed allo-
geneic splenocytes induce donor-specific tolerance for long-
term survival of islet transplants via two distinct mechanisms
Proc Natl Acad Sci USA 200810514527ndash14532
52 Lutterotti A Yousef S Sputtek A et al Antigen-specific
tolerance by autologous myelin peptide-coupled cells
a phase 1 trial in multiple sclerosis Sci Transl Med
20135188ra75
53 Bielekova B SungMH KadomN Simon R McFarland HF
Martin R Expansion and functional relevance of high-avidity
myelin-specific CD41 T cells in multiple sclerosis
J Immunol 20041723893ndash3904
54 Jenkins MK Mueller D Schwartz RH et al Induction
and maintenance of anergy in mature T cells Adv Exp
Med Biol 1991292167ndash176
55 Peschl P Reindl M Schanda K Sospedra M
Martin R Lutterotti A Anti-myelin antibody re-
sponses following induction of antigen-specific toler-
ance with antigen-coupled cells J Neuroimmunol
201427512
56 Tuohy VK Yu M Yin L Kawczak JA Kinkel PR Regres-
sion and spreading of self-recognition during the develop-
ment of autoimmune demyelinating disease J Autoimmun
19991311ndash20
57 Vanderlugt CL Miller SD Epitope spreading in immune-
mediated diseases implications for immunotherapy Nat
Rev Immunol 2002285ndash95
58 Stromnes IM Schmitt TM Chapuis AG Hingorani SR
Greenberg PD Re-adapting T cells for cancer therapy
from mouse models to clinical trials Immunol Rev
2014257145ndash164
10 Neurology Neuroimmunology amp Neuroinflammation
ordf 2016 American Academy of Neurology Unauthorized reproduction of this article is prohibited
DOI 101212NXI000000000000027620163 Neurol Neuroimmunol Neuroinflamm
Larry Steinman Amit Bar-Or Jacinta M Behne et al Restoring immune tolerance in neuromyelitis optica Part I
This information is current as of September 7 2016
2016 American Academy of Neurology All rights reserved Online ISSN 2332-7812Published since April 2014 it is an open-access online-only continuous publication journal Copyright copy
is an official journal of the American Academy of NeurologyNeurol Neuroimmunol Neuroinflamm
ServicesUpdated Information amp
httpnnneurologyorgcontent35e276fullhtmlincluding high resolution figures can be found at
Supplementary Material
httpnnneurologyorgcontentsuppl2016090735e276DC2 httpnnneurologyorgcontentsuppl2016090735e276DC1
Supplementary material can be found at
References httpnnneurologyorgcontent35e276fullhtmlref-list-1
This article cites 58 articles 18 of which you can access for free at
Subspecialty Collections
httpnnneurologyorgcgicollectiontransverse_myelitisTransverse myelitis
httpnnneurologyorgcgicollectionoptic_neuritisOptic neuritis see Neuro-ophthalmologyOptic Nerve
httpnnneurologyorgcgicollectiondevics_syndromeDevics syndrome
httpnnneurologyorgcgicollectionautoimmune_diseasesAutoimmune diseasesfollowing collection(s) This article along with others on similar topics appears in the
Permissions amp Licensing
httpnnneurologyorgmiscaboutxhtmlpermissionsits entirety can be found online atInformation about reproducing this article in parts (figurestables) or in
Reprints
httpnnneurologyorgmiscaddirxhtmlreprintsusInformation about ordering reprints can be found online
2016 American Academy of Neurology All rights reserved Online ISSN 2332-7812Published since April 2014 it is an open-access online-only continuous publication journal Copyright copy
is an official journal of the American Academy of NeurologyNeurol Neuroimmunol Neuroinflamm
15 Hinson SR McKeon A Lennon VA Neurological auto-
immunity targeting aquaporin-4 Neuroscience 2010168
1009ndash1018
16 Sato DK Callegaro D de Haidar Jorge FM et al Cere-
brospinal fluid aquaporin-4 antibody levels in neuromye-
litis optica attacks Ann Neurol 201476305ndash309
17 Steinman L The re-emergence of antigen-specific toler-
ance as a potential therapy for MS Mult Scler 201521
1223ndash1238
18 Roep BO Solvason N Gottlieb PA et al Plasmid
encoded proinsulin preserves C-peptide while specifically
reducing proinsulin specific CD8 T cells in type 1 diabe-
tes Sci Transl Med 20135191ra82
19 Feldmann M Steinman L Design of effective immuno-
therapy for human autoimmunity Nature 2005435
612ndash619
20 Garren H Robinson WH Krasulovaacute E et al Phase 2 trial
of a DNA vaccine encoding myelin basic protein for mul-
tiple sclerosis Ann Neurol 200863611ndash620
21 Bar-Or A Vollmer T Antel J et al Induction of antigen-
specific tolerance in multiple sclerosis after immunization
with DNA encoding myelin basic protein in a randomized
placebo-controlled phase 12 trial Arch Neurol 200764
1407ndash1415
22 Selmi C Autoimmunity in 2012 Clin Rev Allergy
Immunol 201345290ndash301
23 Martin A Tisch RM Getts DR Manipulating T cell-
mediated pathology targets and functions of monoclonal
antibody immunotherapy Clin Immunol 2013148
136ndash147
24 Salvana EM Salata RA Infectious complications associ-
ated with monoclonal antibodies and related small mole-
cules Clin Microbiol Rev 200922274ndash290
25 Madakamutil LT Maricic I Sercarz EE Kumar V
Immunodominance in the TCR repertoire of a TCR
peptide-specific CD41 Treg population that controls
experimental autoimmune encephalomyelitis J Immunol
20081804577ndash4585
26 Buckner JH Mechanisms of impaired regulation by CD4
(1)CD25(1)FOXP3(1) regulatory T cells in human
autoimmune diseases Nat Rev Immunol 201010849ndash
859
27 Linhares UC Schiavoni PB Barros PO et al The ex vivo
production of IL-6 and IL-21 by CD41 T cells is directly
associated with neurological disability in neuromyelitis op-
tica patients J Clin Immunol 201333179ndash189
28 Pohl M Fisher MT Mader S et al Pathogenic T cells
responses against aquaporin 4 Acta Neuropathol 2011
12221ndash34
29 Varrin-Doyer M Spencer CM Schulze-Topphoff U et al
Aquaporin 4-specific T cells in neuromyelitis optica
exhibit a Th17 bias and recognize clostridium ABC trans-
porter Ann Neurol 20127253ndash64
30 Kumar V Sercarz EE The involvement of T cell receptor
peptide-specific regulatory CD41 T cells in recovery from
antigen-induced autoimmune disease J Exp Med 1993
178909ndash916
31 Zhang J T cell vaccination as an immunotherapy for
autoimmune diseases Cell Mol Immunol 20041321ndash
327
32 Huang X Wu H Lu Q The mechanisms and applica-
tions of T cell vaccination for autoimmune diseases a com-
prehensive review Clin Rev Allergy Immunol 201447
219ndash233
33 Fontana R Bregni M Cipponi A et al Peripheral blood
lymphocytes genetically modified to express the selftumor
antigen MAGE-A3 induce antitumor immune responses
in cancer patients Blood 20091131651ndash1660
34 Fox E Wynn D Cohan S Rill D McGuire D
Markowitz C A randomized clinical trial of autolo-
gous T-cell therapy in multiple sclerosis subset anal-
ysis and implications for trial design Mult Scler 2012
18843ndash852
35 Chen G Li N Zang YC et al Vaccination with selected
synovial T cells in rheumatoid arthritis Arthritis Rheum
200756453ndash463
36 Li ZG Mu R Dai ZP Gao XM T cell vaccination in
systemic lupus erythematosus with autologous activated T
cells Lupus 200514884ndash889
37 Leavy O Dendritic cells tracing the origins of cDCs Nat
Rev Immunol 201313703
38 Banchereau J Palucka AK Dendritic cells as therapeutic
vaccines against cancer Nat Rev Immunol 20055296ndash
306
39 Vacchelli E Vitale I Eggermont A et al Trial watch
dendritic cell-based interventions for cancer therapy
Oncoimmunology 201211111ndash1134
40 Mantia-Smaldone GM Chu CS A review of dendritic cell
therapy for cancer progress and challenges BioDrugs
201327453ndash468
41 Segovia-Gamboa N Rodriguez-Arellano ME Rangel-
Cruz R et al Tolerogenic dendritic cells induce antigen-
specific hyporesponsiveness in insulin- and glutamic acid
decarboxylase 65-autoreactive T lymphocytes from type 1
diabetic patients Clin Immunol 201415472ndash83
42 Giannoukakis N Phillips B Finegold D Harnaha J
Trucco M Phase I (safety) study of autologous tolerogenic
dendritic cells in type 1 diabetic patients Diabetes Care
2011342026ndash2032
43 Boks MA Kager-Groenland J Haasjes MSP Zwaginga JJ
van Ham SM ten Brinke A IL-10-generated tolerogenic
dendritic cells are optimal for functional regulatory T cell
induction- a comparative study on human clinical-
applicable DC Clin Immunol 2012142332ndash342
44 Saito M Nagasaw M Takada H et al Defective IL-10
signaling in hyper-IgE syndrome results in impaired gen-
eration of tolerogenic dendritic cells and induced regula-
tory T cells J Exp Med 2011208235ndash249
45 Miller SD Turley DM Podojil JR Antigen-specific tol-
erance strategies for the prevention and treatment of auto-
immune disease Nat Rev Immunol 20077665ndash677
46 Karpus WJ Pope JG Peterson JD Dal Canto MC
Miller SD Inhibition of Theilerrsquos virus-mediated demye-
lination by peripheral immune tolerance induction
J Immunol 1995155947ndash957
47 Kennedy KJ Smith WS Miller SD Karpus WJ Induc-
tion of antigen-specific tolerance for the treatment of
ongoing relapsing autoimmune encephalomyelitis a com-
parison between oral and peripheral tolerance J Immunol
19971591036ndash1044
48 Kennedy MK Tan LJ Dal Canto MC et al Inhibition of
murine relapsing experimental autoimmune encephalomy-
elitis by immune tolerance to proteolipid protein and its
encephalitogenic peptides J Immunol 1990144909ndash
915
49 Turley DM Miller SD Peripheral tolerance induction
using ethylenecarbodiimide-fixed APCs uses both direct
and indirect mechanisms of antigen presentation for
Neurology Neuroimmunology amp Neuroinflammation 9
ordf 2016 American Academy of Neurology Unauthorized reproduction of this article is prohibited
prevention of experimental autoimmune encephalomyeli-
tis J Immunol 20071782212ndash2220
50 Getts DR Turley DM Smith CE et al Tolerance
induced by apoptotic antigen-coupled leukocytes is
induced by PD-L11 and IL-10-producing splenic macro-
phages and maintained by T regulatory cells J Immunol
20111872405ndash2417
51 Luo X Pothoven KL McCarthy D et al ECDI-fixed allo-
geneic splenocytes induce donor-specific tolerance for long-
term survival of islet transplants via two distinct mechanisms
Proc Natl Acad Sci USA 200810514527ndash14532
52 Lutterotti A Yousef S Sputtek A et al Antigen-specific
tolerance by autologous myelin peptide-coupled cells
a phase 1 trial in multiple sclerosis Sci Transl Med
20135188ra75
53 Bielekova B SungMH KadomN Simon R McFarland HF
Martin R Expansion and functional relevance of high-avidity
myelin-specific CD41 T cells in multiple sclerosis
J Immunol 20041723893ndash3904
54 Jenkins MK Mueller D Schwartz RH et al Induction
and maintenance of anergy in mature T cells Adv Exp
Med Biol 1991292167ndash176
55 Peschl P Reindl M Schanda K Sospedra M
Martin R Lutterotti A Anti-myelin antibody re-
sponses following induction of antigen-specific toler-
ance with antigen-coupled cells J Neuroimmunol
201427512
56 Tuohy VK Yu M Yin L Kawczak JA Kinkel PR Regres-
sion and spreading of self-recognition during the develop-
ment of autoimmune demyelinating disease J Autoimmun
19991311ndash20
57 Vanderlugt CL Miller SD Epitope spreading in immune-
mediated diseases implications for immunotherapy Nat
Rev Immunol 2002285ndash95
58 Stromnes IM Schmitt TM Chapuis AG Hingorani SR
Greenberg PD Re-adapting T cells for cancer therapy
from mouse models to clinical trials Immunol Rev
2014257145ndash164
10 Neurology Neuroimmunology amp Neuroinflammation
ordf 2016 American Academy of Neurology Unauthorized reproduction of this article is prohibited
DOI 101212NXI000000000000027620163 Neurol Neuroimmunol Neuroinflamm
Larry Steinman Amit Bar-Or Jacinta M Behne et al Restoring immune tolerance in neuromyelitis optica Part I
This information is current as of September 7 2016
2016 American Academy of Neurology All rights reserved Online ISSN 2332-7812Published since April 2014 it is an open-access online-only continuous publication journal Copyright copy
is an official journal of the American Academy of NeurologyNeurol Neuroimmunol Neuroinflamm
ServicesUpdated Information amp
httpnnneurologyorgcontent35e276fullhtmlincluding high resolution figures can be found at
Supplementary Material
httpnnneurologyorgcontentsuppl2016090735e276DC2 httpnnneurologyorgcontentsuppl2016090735e276DC1
Supplementary material can be found at
References httpnnneurologyorgcontent35e276fullhtmlref-list-1
This article cites 58 articles 18 of which you can access for free at
Subspecialty Collections
httpnnneurologyorgcgicollectiontransverse_myelitisTransverse myelitis
httpnnneurologyorgcgicollectionoptic_neuritisOptic neuritis see Neuro-ophthalmologyOptic Nerve
httpnnneurologyorgcgicollectiondevics_syndromeDevics syndrome
httpnnneurologyorgcgicollectionautoimmune_diseasesAutoimmune diseasesfollowing collection(s) This article along with others on similar topics appears in the
Permissions amp Licensing
httpnnneurologyorgmiscaboutxhtmlpermissionsits entirety can be found online atInformation about reproducing this article in parts (figurestables) or in
Reprints
httpnnneurologyorgmiscaddirxhtmlreprintsusInformation about ordering reprints can be found online
2016 American Academy of Neurology All rights reserved Online ISSN 2332-7812Published since April 2014 it is an open-access online-only continuous publication journal Copyright copy
is an official journal of the American Academy of NeurologyNeurol Neuroimmunol Neuroinflamm
prevention of experimental autoimmune encephalomyeli-
tis J Immunol 20071782212ndash2220
50 Getts DR Turley DM Smith CE et al Tolerance
induced by apoptotic antigen-coupled leukocytes is
induced by PD-L11 and IL-10-producing splenic macro-
phages and maintained by T regulatory cells J Immunol
20111872405ndash2417
51 Luo X Pothoven KL McCarthy D et al ECDI-fixed allo-
geneic splenocytes induce donor-specific tolerance for long-
term survival of islet transplants via two distinct mechanisms
Proc Natl Acad Sci USA 200810514527ndash14532
52 Lutterotti A Yousef S Sputtek A et al Antigen-specific
tolerance by autologous myelin peptide-coupled cells
a phase 1 trial in multiple sclerosis Sci Transl Med
20135188ra75
53 Bielekova B SungMH KadomN Simon R McFarland HF
Martin R Expansion and functional relevance of high-avidity
myelin-specific CD41 T cells in multiple sclerosis
J Immunol 20041723893ndash3904
54 Jenkins MK Mueller D Schwartz RH et al Induction
and maintenance of anergy in mature T cells Adv Exp
Med Biol 1991292167ndash176
55 Peschl P Reindl M Schanda K Sospedra M
Martin R Lutterotti A Anti-myelin antibody re-
sponses following induction of antigen-specific toler-
ance with antigen-coupled cells J Neuroimmunol
201427512
56 Tuohy VK Yu M Yin L Kawczak JA Kinkel PR Regres-
sion and spreading of self-recognition during the develop-
ment of autoimmune demyelinating disease J Autoimmun
19991311ndash20
57 Vanderlugt CL Miller SD Epitope spreading in immune-
mediated diseases implications for immunotherapy Nat
Rev Immunol 2002285ndash95
58 Stromnes IM Schmitt TM Chapuis AG Hingorani SR
Greenberg PD Re-adapting T cells for cancer therapy
from mouse models to clinical trials Immunol Rev
2014257145ndash164
10 Neurology Neuroimmunology amp Neuroinflammation
ordf 2016 American Academy of Neurology Unauthorized reproduction of this article is prohibited
DOI 101212NXI000000000000027620163 Neurol Neuroimmunol Neuroinflamm
Larry Steinman Amit Bar-Or Jacinta M Behne et al Restoring immune tolerance in neuromyelitis optica Part I
This information is current as of September 7 2016
2016 American Academy of Neurology All rights reserved Online ISSN 2332-7812Published since April 2014 it is an open-access online-only continuous publication journal Copyright copy
is an official journal of the American Academy of NeurologyNeurol Neuroimmunol Neuroinflamm
ServicesUpdated Information amp
httpnnneurologyorgcontent35e276fullhtmlincluding high resolution figures can be found at
Supplementary Material
httpnnneurologyorgcontentsuppl2016090735e276DC2 httpnnneurologyorgcontentsuppl2016090735e276DC1
Supplementary material can be found at
References httpnnneurologyorgcontent35e276fullhtmlref-list-1
This article cites 58 articles 18 of which you can access for free at
Subspecialty Collections
httpnnneurologyorgcgicollectiontransverse_myelitisTransverse myelitis
httpnnneurologyorgcgicollectionoptic_neuritisOptic neuritis see Neuro-ophthalmologyOptic Nerve
httpnnneurologyorgcgicollectiondevics_syndromeDevics syndrome
httpnnneurologyorgcgicollectionautoimmune_diseasesAutoimmune diseasesfollowing collection(s) This article along with others on similar topics appears in the
Permissions amp Licensing
httpnnneurologyorgmiscaboutxhtmlpermissionsits entirety can be found online atInformation about reproducing this article in parts (figurestables) or in
Reprints
httpnnneurologyorgmiscaddirxhtmlreprintsusInformation about ordering reprints can be found online
2016 American Academy of Neurology All rights reserved Online ISSN 2332-7812Published since April 2014 it is an open-access online-only continuous publication journal Copyright copy
is an official journal of the American Academy of NeurologyNeurol Neuroimmunol Neuroinflamm
DOI 101212NXI000000000000027620163 Neurol Neuroimmunol Neuroinflamm
Larry Steinman Amit Bar-Or Jacinta M Behne et al Restoring immune tolerance in neuromyelitis optica Part I
This information is current as of September 7 2016
2016 American Academy of Neurology All rights reserved Online ISSN 2332-7812Published since April 2014 it is an open-access online-only continuous publication journal Copyright copy
is an official journal of the American Academy of NeurologyNeurol Neuroimmunol Neuroinflamm
ServicesUpdated Information amp
httpnnneurologyorgcontent35e276fullhtmlincluding high resolution figures can be found at
Supplementary Material
httpnnneurologyorgcontentsuppl2016090735e276DC2 httpnnneurologyorgcontentsuppl2016090735e276DC1
Supplementary material can be found at
References httpnnneurologyorgcontent35e276fullhtmlref-list-1
This article cites 58 articles 18 of which you can access for free at
Subspecialty Collections
httpnnneurologyorgcgicollectiontransverse_myelitisTransverse myelitis
httpnnneurologyorgcgicollectionoptic_neuritisOptic neuritis see Neuro-ophthalmologyOptic Nerve
httpnnneurologyorgcgicollectiondevics_syndromeDevics syndrome
httpnnneurologyorgcgicollectionautoimmune_diseasesAutoimmune diseasesfollowing collection(s) This article along with others on similar topics appears in the
Permissions amp Licensing
httpnnneurologyorgmiscaboutxhtmlpermissionsits entirety can be found online atInformation about reproducing this article in parts (figurestables) or in
Reprints
httpnnneurologyorgmiscaddirxhtmlreprintsusInformation about ordering reprints can be found online
2016 American Academy of Neurology All rights reserved Online ISSN 2332-7812Published since April 2014 it is an open-access online-only continuous publication journal Copyright copy
is an official journal of the American Academy of NeurologyNeurol Neuroimmunol Neuroinflamm
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is an official journal of the American Academy of NeurologyNeurol Neuroimmunol Neuroinflamm