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Semen Semen EvaluationEvaluation

ผศ.น.สพ . ชยัวฒัน์ จรสัแสงภาควชิาศัลยศาสตรแ์ละวทิยาการสบืพนัธุ์

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Semen collectionSemen collection

• Semen collectorSemen collector• Bull handlerBull handler• Teaser handlerTeaser handler

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Semen collectionSemen collection

• Artificial vaginaArtificial vagina

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Semen collectionSemen collection

• Electro-ejaculatorElectro-ejaculator

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Semen collectionSemen collection : manual : manual manipulationmanipulation

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Semen Semen evaluationevaluation•Macroscopic Macroscopic evaluationevaluation

•Microscopic evaluationMicroscopic evaluation• Special evaluationSpecial evaluation

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Macroscopic e Macroscopic evaluationvaluation

•VolumeVolume Beef bull 5 -Beef bull 5 -10 ml10 ml

Dairy bull Dairy bull 10-15 ml10-15 ml

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2. 2.ConsistencyConsistency

•Watery Watery •Translucent Translucent •Milky Milky •Creamy Creamy •Glue -likeGlue -like

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3. Density 3. Density•D = D = thinthin •DD = mediumDD = medium•DDD = thick DDD = thick

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4 . pH 4 . pH•Beef bull 6.7Beef bull 6.7•Elephant bull Elephant bull 7.047.04

•pH meter or pH pH meter or pH paperpaper

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5 . Contamin 5 . Contaminaaaaaaaaaa

•Pus Pus •BloodBlood•hair hair •Urine Urine

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6 .Color6 .Color• Transparent gr Transparent graa y y• Evident gr Evident graa y y• Strong white , Yellowish whit Strong white , Yellowish whit

e e•YellowYellow

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Microscopic E Microscopic Evaluationvaluation•1.1. Mass Activity Mass Activity

10 10 XX0 = NO motility0 = NO motility+ = + = มีมี คล่ืน แต่ไมรุ่นแรงนัก คล่ืน แต่ไมรุ่นแรงนัก++ = ++ = เรว็และแรง หมุนชา้ ๆ เรว็และแรง หมุนชา้ ๆ

+++ +++ = = เป็นคลื่น หมุนรุนแรง เป็นคลื่น หมุนรุนแรง

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2. Motility2. Motility• - 1040 X with C- 1040 X with C

over glass over glass• Progressive move Progressive movementment –% motile sperm % motile sperm

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33 . Concentrat . Concentrationion

• HHaa emocytometer method emocytometer method• Flow cytometryFlow cytometry• Electronic particle counterElectronic particle counter• DNA assayDNA assay• Computer-assistedComputer-assisted semen analyzerssemen analyzers

(CASA)(CASA) • Turbidity Turbidity

-- Photolectric colorimeter Photolectric colorimeter - Spectrophotometer- Spectrophotometer

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HaemocytometHaemocytomet er er mmethodethod- Formal saline dilute Formal saline dilute Boar 1 : 5 0 Boar 1 : 5 0

1200Bull : 1200Bull : Stallion 1 : 1 0 0 Stallion 1 : 1 0 0

- 4 0 X- 4 0 X - (NxDx 2 5 0- (NxDx 2 5 0 ) / A ) / A mmmm33

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4. Percentage of li 4. Percentage of liaaa aaaaaaaa aaaaa• Eosin ,Nigrosin, Eosin ,Nigrosin, Fast Fast

green green analine blue analine blue• 100 x 100 x•200-200- 5 0 0 sperm 5 0 0 sperm

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5. Sperm morp 5. Sperm morpaaaaaaaaaaaa

• --- Dry preparation > H --- Dry preparation > H ead of sperm ead of sperm

Indian ink Indian ink -Eosin Nigrosin -Eosin Nigrosin -Geimsa stain -Geimsa stain Wright’s stain Wright’s stain William stain William stain

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Sperm morpho Sperm morphoaaaaaaaa

• --- Wet preparation > --- Wet preparation > tail of sperm tail of sperm

• Formal saline 1:2 ,1:5 Formal saline 1:2 ,1:5

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Classification of Classification of Abnormal Sperm Abnormal Sperm

• Primary abnormality ,S Primary abnormality ,S econdary econdary

abnormalityabnormality• Major or Minor sperm Major or Minor sperm

abnormalityabnormality

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Major sperm a Major sperm abnormalitybnormality

Proximal cytoplasmic d Proximal cytoplasmic dropletroplet Pyriform head Pyriform head

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Double headed sperm Double headed sperm

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Strongly fold ,coile d tail ,coiled around

the head

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Middle piece defects

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Maldeveloped or undelveloped

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Diadem defect Diadem defect or Pouch forma or Pouch forma

tiontion

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Minor sperm a Minor sperm abnormalitybnormality

- - Distal cytoplasmic drop Distal cytoplasmic dropletslets

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Tailess normal heads and detached sperm head

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Simple bent tail or Terminally coiled tail

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Narrow ,small ,Giant head

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Abaxial implantation

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Abnormal acrosome Abnormal acrosome

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Special sperm evaluationSpecial sperm evaluation• Metabolic reaction ratesMetabolic reaction rates

– GlycolysisGlycolysis– RespirationRespiration

• Hydrogen ion concentrationHydrogen ion concentration• Penetration hamster ovaPenetration hamster ova• FiltrationFiltration• Sexing semen Sexing semen • etcetc