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Sidho-Kanho-Birsha University Ranchi Road, P.O.- Sainik School

District - Purulia, PIN – 723104, W.B.

Phone : 03252- 202419 Website-www.skbu.ac.in

Ref No: FO/324 /SKBU/17 Date: 01/06/2017

E- Tender Notice

1. SIDHO-KANHO-BIRSHA University, Purulia intends to procure Lab Instruments for

Zoology Department at Purulia. The tentative quantity of the required items along

with technical configuration of each items are mentioned at Annexures separately. SKB

University is looking for interested bidders who have experience in supplying of above

type of instruments.

2. Bidders are advised to study all technical and financial aspects, instructions, forms,

terms and specifications carefully in the tender document. Failure to furnish all

information required in the Tender Document or submission of a bid not substantially

responsive to the Tender document in every respect will be at the Bidder’s risk and may

result in the rejection of the bid.

3. Intending bidder may download the tender documents from the website

https://wbtenders.gov.in OR www.skbu.ac.in. Only Online Submission of Tender

will be accepted. Last date of submission: 20/06/2017 up to 12:00 hrs. Opening

Date 22/06/2017, 1 PM.

4. The categories of items and quantity indicated in the Tender Document are

tentative. SKB University (SKBU), however, reserves the right to increase or decrease

the quantity or delete some or all of the items depending on the needs of the SKBU and

availability of funds without assigning any reasons.

5. The bidder should indicate specifically the Basic Price, Taxes, VAT, Entry tax,

other duties (if any), and levies chargeable quantitatively against each item.

University will assist to have way bill (if necessary) but the pay will be borne

by the bidder. University having valid Central excise duty exemption certificate issued

by DSIR, New delhi..No additional information will be entertained after due date. SKBU

may reject bids if they do not carry such information separately and specifically

quantitatively.

6. The tender should be submitted in two cover system (electronically) as defined in E-

Tender participation system: (a) Technical Bid (b) Financial Bid.

7. The bidder should clearly indicate the delivery period and validity period of tender.

8. The bidder should clearly indicate the availability of service and maintenance facilities

at Purulia for the items quoted.

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9. The bidders are required to quote for each item separately in terms of basic price

and all other charges. Prices can be quoted in Multi Currency.

10. The bidder (Technical Bid) must be submitted along with the copies of OEM license

or authority from the manufacturer.

11. SKB University reserves the right to reject any or all tenders without assigning any

reason whatsoever.

12. No advance payment or payment against Performa invoice will be made. Payment

will be made after receipt, inspection, and installation/testing.

13. All damaged or unapproved goods shall be returned at the bidder’s risk and cost

and the incidental expenditure thereupon shall be recovered from the concerned party.

14. On acceptance of tender, the date of delivery should be strictly adhered to

otherwise, the SKB University reserves the right not to accept the delivery in full or in

part. In case the order is not executed within the stipulated period, the SKB University

will be at liberty to make purchases through other sources.

15. Payment of bill will be made through by crossed account payee Cheque or electronic

payment (NEFT) only after delivery and successful installation of each of the items.

16. Delivery Schedule: The Company shall be able to deliver the required items within

15 days of the receipt of order. Delivery/Installation is to be done at Purulia, West

Bengal.

17.Warranty : All the products must carry minimum one year comprehensive warranty.

18. The products asked for should be of very high standard and of mentioned brand.

19. Liquidated Damages : The Company shall be liable to indemnify the SKB

University in all respects and meet and pay off the litigation expenses and all the

liabilities including damages, sums etc. arising out of and as a consequence of the

negligence, deficiencies, mistakes, lapses, delays etc. in the execution of the various

jobs and the services provided.

20. The company should ensure quick back up response in case of equipment failure

which should be replaced if needed within 48 hours of the distress call.

21. Payment: There is no provision for making advance payment to the Company.

However, the running bills for the jobs completed can be submitted by the company

and will be cleared for payment within reasonable period.

22. Purchase Order: The purchase order for the entire quantity can be placed either

in one lump sum or as per the requirement through repeat order subject to availability

of fund of the required items. The quantity shown is tentative and may increase or

decrease.

Registrar

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Annexure 1

Proforma For Techncial Bid

SL Particulars Information

1 Name of the Firm & Owner : (with Telephone/Mobile Numbers, email)

2 Type of Organisation (Proprietorship/Partnership/Pvt. Ltd./Limited Company) Month/Year of Establishment

3 Website of Bidder, if any

4 Month/Year of Establishment

5 PAN/Sales Tax/VAT Regd. Nos.

6 Annual Turnover 2016-17

7 Whether product(s) offered by the bidders are exactly as per the configuration of SKB University. If not, indicate the changes in each product

8 Clientele (submit copies)

9 Validity of Tender

10 Whether Terms & Conditions issued by SKB University are acceptable to the Firm

11 Whether Warranty as per the desired specification

** Bidder should only quote rate for 1 unit of each item in BOQ. Date Name: Signature of Owner/Authorized Representative Designation:

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Technical Specification Technical Specification For e-tender

SL

NO Item Specification Quantity

1

Bench Top Compact High

Speed cell sorter and Flow

based analyzer

1. Bench top flow cytometer Cell Sorter required with 488 nm

/equivalent blue Laser, 633-642 nm /equivalent Red laser and

405 nm / equivalent Violet Laser.

2. The system should be based on cuvette based fixed aligned cell

sorting to avoid any user level alignment while day to day run

of the instrument.

3. The system should have at least 8 fluorescence/colour (10

parameters) measurement capability simultaneously,

upgradable to more colours.

4. System should have minimum3 beam spots without any

customization with the base instrument.

5. The system must have nozzle of size of 100 microns and the

nozzle tip can be removed during operation, replaced and

stream optimized.

6. No manual alignment of nozzle to be done by the user even after

removal and replacement of the nozzle into the system.

7. The system should be able to acquire & sort at least 25,000 or

more -events/second.

8. The system should have option of automatic cell deposition unit

which allows for slide and plate sorting into 6, 24, 48, 96 and

384 plates.

9. Viability & yield should be more than 90% in routine

applications

10. Date Management system : Latest PC Workstation from source,

with compatible monitors

11. The company should offer the latest and updated model and

should have a dedicated training centre with documented proof

of conducting regular training for research applications.

12. The system should be space saving and compact model

13. System must have upgradability option with Bio-safety cabinet.

.

14. Warranty: 1 year from the date of installation

15. Starter Kit for installation and 3 KVA Online UPS & colour

laser Printer should be supplied with the system.

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16. System should be installed with minimum 3 years of

comprehensive maintenance contract ( CMC) from

manufacturer.

17. Minimum 40 lt of sheath fluid should be provided at the time of

installation.

18. At least 6 Fluorescence labelled antibody of choice must be

included in the package.

2

Mini- 1-D vertical gel

electrophoresis system.

The cell should be durable, versatile, and easy to assemble,

and run up to four precast or handcast polyacrylamide gels.

Runs 1–4 mini polyacrylamide gels in 30 min

Leak-free electrophoresis and gel casting

I moulded polycarbonate construction

Ability to run either precast or hand cast gels

Gel size 8.6x 6.8

Buffer volume for 4 gels-1000ml

3

3

Universal power supply

system for horizontal DNA

gels electrophoresis and

vertical acrylamide gel

electrophoresis of proteins

. Should have simple programming and a compact, stackable

case. timer control, constant voltage or constant current output,

real-time monitoring of both voltage and current, and

pause/resume run capability.

Runs four cells simultaneously

Timer controlled

Fully programmable

CE and EN-61010 safety compliant

Case stackable

Voltage10-500V

Current -0.01-2.5 Amp

Power-1 -500W

3

4

Mini wet transfer system

Transfer two 10 x 7.5 cm gels simultaneously

High field strength for rapid 1 hr transfers

Should be able to run overnight at low voltage

Wire electrodes should be placed at least 4 cm apart for strong

electrical fields and efficient protein transfer

Colour-coded cassettes and electrodes for proper orientation of

the gel during transfer

Should have Ice cooling unit, completely contained within the

Mini Blot cell, for absorbing heat generated during rapid

transfers

Buffer-450ml

2

5

Bench-top high speed mini

centrifuge

Capacity: 24 x 1.5/2 mL

Max speed: 13,000 rpm

Max RCF: 17,000xg

Type: Benchtop centrifuge

Run time: 1 to 99 min

Temperature range: Ambient temperature

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6

Cyto centrifuge system

Should be bench-top

Rotor should be able to accommodate 12 glass microscopic

slides.

Should be able to carefully controlled centrifugation to

separate and deposit a thin layer of cells on slides while

maintaining cellular integrity.

1

7

Magnetic Stirrer

Speed: 100 to 1700 rpm (Constantly adjustable)

Maximum stirring capacity: 10L

Temperature control: room temperature to 199°C

(Constantly adjustable)

Highest temperature: 300°C

4

8

Water Bath

Specifications:

Capacity: 6, 10, 20L

Heating temperature range: 5°C to 100°C

Temperature accuracy: ±0.2°C

1

9

Vortex

Specifications:

Variable Speed Control

Maximum Speed: 3000 RPM

Choice of Continuous & Touch Mode

Rubber Feet

4

10

Dancing Shaker

Specifications:

Three dimensional combinations of rocking and orbital

motion,

Non slip platform: adjustable from horizontal to a steep angle

continuously,

Low foaming agitation and uniform mixing capacity at slow

speed,

Speed limit: 0-60 RPM.

4

11

PH meter

Microprocessor based, Ph range 1-14

Digital display, touch based calibration and measurement

button. systems that will measure pH, ion concentration (ISE),

conductivity, dissolved oxygen, temperature, or a combination

of parameters.

4

12

CO2 Incubator Co2 incubator for mammalian cell culture, temp range 37

degree, chip based temperature and CO2 control. 3 rack, outer

body corrosion proof, should have UV for sterilization.

1

13

Micropipette

Fully autoclavable, 0.2 -10 ul, 2-20ul, 20-200ul, 200-1000ul

and 5000ul

3 each

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14

Multichannel pipette

Should have 12 channels, 20-300 ul.

2

15

FLUORESCENCE

SPECTROPHOTOMETER

FEATURES:

System must have a built in high resolution large LCD

display and should have provision for PC communication.

Microprocessor controlled Fluorescence spectrophotometer

must be effective for 3-D measurement and realtime

auto-scale function with a unique high-efficiency diffraction

grating.

A small amount of 1 x 10-12 mol/L fluorescein should be

detected by the low fluorescence intensity of range from

0.001 to 9999.

Should have option for quantum yield measurement.

System should be satisfied Cutting-Edge Fluorescence

analysis.

Should have automatic pre scan and compatible for GFP

measurement and DDE, OLE functions.

The dynamic range Should be at least 6 order magnitude or

more

Should have a JIS K 0120 satisfied performance check

function.

Should have monochromatic light monitoring ratio

calculation function.

SPECIFICATIONS:

Measurement techniques : 3-dimensional Fluorescence measurement, automatic sensitivity measurement, measurement of wavelength accuracy and wavelength setting repeatability. prescan, Data storage capacity(minimum up to 50)

Spectral Range : Should 220-730nm, with 0-order light Extended up to 800nm , with optional PMT detector

Spectral Bandwidth : 2.5, 5, 10, 20nm (Both excitation and emission)

Sensitivity : High sensitivity with S/N 10000 or better (RMS) using Raman band of water (Peak to Peak) Excitation wavelength 350nm, bandwidth 5 nm, response 2s.

Light source : Self-deozonating Xenon flash lamp 150 W

Detector : High Performance Photomultiplier Tube(R928F)

Monochromator : Aberration-corrected stigmatic concave diffraction mechanical grating instead of Holographic grating Brazed wavelength : Excitation side 300nm,(emission side) 400nm (fluorescence side). Half value width calculation with spectrum averaging

Wavelength pre scan speed : Should 1500nm/min.

Wavelength drive speed : Maximum 12000 nm/min

Wavelength Accuracy : ±2 nm

Wavelength Repeatability : ≤ 1nm

Photometric value range : -9999 to 9999

Wavelength scan function : Provision for synchronous spectra/repetitive measurement/CAT, Linear to

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biquadratic differentiation, trace, scale conversion, smoothing etc

Working temperature / humidity : 15 to 35ºC, 45 to 80% (condensation not allowed, 70% or less at 35ºC or higher)

Dimensions / weight : Spectrophotometer: 620 W x 520 D x 300 H mm (excluding protrusions)/ 41 kg

Software should have provision for controlling the instrument & its accessories & 3-dimensional time scan measurement and Data processing features such as quantitative analysis, cumulative data averaging, first to fourth order differentiation, statistic calculation, graph axis change, smoothing, area calculation ,spectrum normalization ,wavelength scan measurement, time based measurement such phosphorescence life time, 3-D measurements, Data export to Microsoft Excel etc.

All the above specifications should be mentioned in printed

literature of manufacturer

Company should have factory trained engineer & Kolkata

based service station.

16

ICE FLAKING MACHINE

WITH BUILT-IN-BIN

Microprocessor controlled fully automatic Ice Flaking

machine.

Air cooled system

CFC Free Refrigeration system.

Ice faker body is made of non corrosive steel and well

electrically grounded for safety.

Auto shut off if water temperature is high

Built in visual alarm

Stainless steel insulated storage bin.

Auto shut off when bin is full.

Auto shut off of low water pressure

Continuous crushed ice production facility.

Electronic controlled system.

Highly hygienic bin for storage of ice.

Low power and water consumption.

Attractive drain hose

Noise free compressor

Production capacity : 70 kg /24hrs

Bin capacity : 25 kg

1

17

ULTRA SONICATOR

SYSTEM

Compact ultrasonic processor

Power: 200 Watts

Frequency : 24 kHz

Capacity: Up to 2000 ml.

Automatic frequency scanning system,

Amplitude adjustable 20-100%, pulse adjustable 0-100%.

Dry running protection.

Solid titanium probe for all sample even for organic solvents

or low surface tension liquids. Replaceable tip or sonotrode

with multiple joint will not be entertained.

Easy positioning of samples under the ultrasonic probes to

control immersion depth, stainless steel.

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18

Inverted Microscope with

Bright Field, DIC

&Fluorescence

Stand ergonomically designed, rigid and shock proof. Controls

for light path selector, transmitted light intensity and ON/OFF

switch.

Bright Field, DIC and Fluorescence

Universal/Long working distance condenser with 6/7 position

and with minimum working distance of 27-28mm

Coded nosepiece for easy operation, Features must be high

grade smooth operation and with positive click stops

Manual XY Stage with universal sample holder for plates,

glass slides, petri plates etc.

The microscope should be provided with a DIC slot, a

polarizer and an analyser. It should have complete DIC

accessories for the objectives specified.

10X/22mm field of view or better

LED illumination with minimum life of 40000hrs

Binocular type observation tube

Plan Objectives 5x, 10X (NA 0.25), 20X (NA 0.40),

Fluorescence grade CORR Objective 40 X (NA 0.5-0.6mm)&

Fluorescence grade 100x (NA 1.3)

Coded filter turret with 6 or more position.

Long Life cold LED Light Source with minimum 20000 Hrs

life span

Filter for DAPI, FITC, Rhodamine

Cooled fluorescence colour camera with USB3.0 interface.

Ideally suited for both- low light fluorescence application and

brightfield imaging with superb colour reproduction. Colour

CCD sensor with desired technology to offer exceptionally

high speed of acquisition combined with high sensitivity even

in the near infrared region. The camera should have minimum

1920 x 1440 pixel, 4.5 µm x 4.5 µìm pixel size and a typical

full well capacity should be minimum of 15.000 electrons.

Partial scan, various binning modes (color and monochrome),

and overlapping mode for high-speed imaging should be

available.

Max. Frames per second should be minimum of 40 fps in full

frame. Suitable C-mount for Side Port should be. The camera

make should be from the same manufacturer as that of the

Microscope.

Image capture and analysis software and should include It

should have multi-channel fluorescence capturing and

merging, point to point measurement, annotation and scale

bar.No third party Software for imaging system will be

accepted.

The Microscope should be accompanied by an Imaging

/Computer system having a configuration of no less than Intel

i5 processor, 4GB RAM, 1TB HDD, minimum display 18

inch.1 KVA UPS should also be provided for the system(s).

Warranty of at least 1 year from the date of installation

1

19

GCMS

GC:

1. Oven temp. Upto 4500C

2. Injector port temp. Upto 4500C

3. AFC pr. Range: 0 to 970 Kpa

MS:

1. Type: Direct connection with capillary column 50

2. Temp: to 3500C

3. Ion source temp: 140-2600C

4. Filament: Dual (Automatic switching)

5. Electron energy: 10-200 eV

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6. Emission current: 5 - 250µA

Mass analyser and Detector:

1. Mass analyser: Metal quadrupole mass filter with pre rod

2. Mass range: m/z 1.5 – 1000

3. Mass resolution: 2M (FWHM)

4. High speed scan rate: 10,000µ/sec

5. Minimum measurement interval: 0.01s (maximum

100scan/sec)

6. Detector: Secondary electron multiplier with the patented

overdrive lens and conversion dynode.

7. Main Pump: Turbomolecular pump (58L/sec for He)

8. Force Pump: 30L/min (60Hz) Rotary Pump

9. Column Flow: upto 4mL/min (He)

Soft ware (GCMS soln. Ver 2.6)

Mode- Scan, SIM, FASST

SIM-64 channels x 128 group

Energy saving- Ecology Mode

Method creation: COAST (Creation automatic SIM Table)

AART (Automatic Adjustment Retention time)

Instrument tuning: Autotuning for EI modery of

Library of compounds for identification and matching .

20

Water Flow meter (Digital)

Nominal Dia (mm): 15-400

Electrode material: MS, SS 304, SS316

Process pressure-Max 25kg/cm2

Flow tube material: SS 316

End connection- Flange

Measuring range-0.5m/sec for minimum and 5m/sec for

maximum

Display- 2 Line Alfa Numeric LCD

Display Unit- Standard Unit in m3, lit

Power supply: 230VAC, 110VAC

Output: Std 4- 20mA

Protection clas for sensor- IP 65

Protection clas for transmitter-IP 65

Cable length for remote- 10mts

Installation – Inline Flange type

1

21

Refrigerated Bench Top

centrifuge:

Control: Microprocessor

Capacity: 4x400ml

Max, RCF: 25830xG

Max speed: 15,200 rpm

1

22

Amino acid analyser:

Column: 4.6mmD, 60mmL

Pump: Delivery pressure 0 to 20 MPa

Flow rate range: 0.000 to 0.999 mL/min.

Auto sampler: Injection method- Direct injection method

Sample vial capacity- 1500µL

No. Of vial accommodated- 200

Sample injection Vol: 0.1 to 100 µL

Column Oven: Temp. Setting range: 20-850C

Photometer: Spectrophotometer. A planatic concave

diffraction grating

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23

Portable Dissolved oxygen

meter:

1. Polarographic measuring system

2. Automatic calibration

3. Auto temp. Compensation-0-500C

4. Altitude compensation: 0 to 4000mts with a 100mt.

Resolution

5. DO range: 0 to 45 mg / l

6. DO resolution: 0.01mg/l

7. Temp. Range: 0 to 500C

8. Salinity compensation: 0 to 80 g/l

9. Electrode /Probe: Polarographic DO probe

10. Battery type: 1.5 VAAA(3)

11. Dimensions: 185x72x36mm

12. Wt. 300g

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24

Fully Automated Rotary

Microtome

1. Section thickness setting: 0.5 – 100 µm

2. Trimming section thickness setting – 1 – 600 µm

3. Vertical stroke – 70 mm/2.76 inches

4. Reposition of knife holder base:

5. Horizontal: ±24mm/0.94 inches

6. Vertical: ±23mm/0.91 inches

1

25

Upright trinocular research

microscope with

transmitted light bright

field, phase contrast &

Fluorescence & DIC

application along with High

resolution color CCD

camera & software

1) Microscope should be suitable for Bright Field, Dark Field,

Phase Contrast, Fluorescence & DIC application. Also should

be dual camera attachment facility.

2) Transmitted light halogen lamp for 12V/100 watt for

observation and photomicrography under bright field & phase

contrast application, DIC application along with a spare lamp.

3) Binocular phototube with dual camera facility should be 20º

for better ergonomic & (100:0/0:100) for observation under

bright field, fluorescence & DIC illumination.

4) Widefield focusable 10x eyepieces with larger field of view

should be 23 -25 mm or higher for observation under

fluorescence & DIC image.

5) 6x revolving nosepiece (accommodate 6 objectives at a time).

6) Infinity color contrast corrected optical system based objective

5x, 10x, 20x, 40x, 63x, 100x (oil)

should be Fluorite objective with DIC facility & 100x should

be high N.A 1.30.

7) Magnification changer 1.25 x / 1.6x / 2.5x / 4.0x should be

available with push and click module. Fluorescence & DIC

images as well as photographic images can be magnified.

8) Achromatic aplanatic universal turret type condenser 0.9 for

for bright field, Phase contrast & DIC application

9) Large circular disc type Intensity regulator & focusing knob

placed both side ,accessible from both side.

10) Microscope stand should be built in 6-position filter wheel

equipped with filter slider at the back for -Interferance green

filter, white balance filter, contrast enhancing blue filter,

Neutral Density filter 0.25 & Neutral density 0.06, daylight

conversion filter for contrast enhancement for transmitted light

application.

11) Fluorescence reflector turret should be six position for

fluorescence filters.

12) The burner should be removable with the drawer – for a

particularly easy and comfortable change of burner.

Fluorescence Filter with patented stray light trap system. filters

are insertable and removable with push & click method

without any need of tools.

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100 watt mercury vapour lamp for fluorescence illumination

with fluorescence filters

Fluorescence Filter Excitation shift free EX BP 450-490,

Emission filter LP 515

Fluorescence Filter Excitation BP 546/12, emission LP 590

Fluorescence Filter UV Excitation shift free EX G 365, with

antiglare screen

Photography system:

Basic CCD resolution 6.0 MP, pixel pitch should be 4.65µm X

4.65µm or better,

Chip size: 12 mm x 10 mm, equivalent to 1" (16 mm

diagonal), monochrome CCD,

Spectral range: with protection glass app. 400 nm to 1000 nm,

binning upto 5x5

Live frame rates (depending on hardware and software

configuration):

Exp. Time 250 ms to 60s, Frame Rate 51 fps, Cooling: One

stage Peltier cooling, sensor temperature

Digitaization 14 bit/pixels, image sensor 2/3”, live image

frame rate 15 max. (on resolution upto 1920 x 1080) or better,

exposure time: 1 ms to 4s, dynamic range:- approx. 1:500 &

imaging software for live image preview, multi channel

fluorescence, multi focusing module, interactive measurement,

time lapse, shading correction, color adjustment, white balance

settings, text annotation etc complete with CCD C-mount

adapter 0.63x compatible trinocular microscope completed

with a suitable Laptop/Desktop (Lenovo/Dell/HP with Intel

core processor, 6th Gen, 8 GB DDR4, 1TB HDD, 2-4 GB

graphics card, 14-15 inch screen) to supplied along with the

system to ran the CCD camera & software

2626

26

Trino Inverted Cell Culture

Microscope for Bright Field

& Phase Contrast with

photography system

1) Inverted Microscope with 6V/30-35 watt halogen illumination

as well as long lasting LED

lamp attachment for observation and photography under

bright field & phase contrast.

Microscope should have binocular phototube 45°/20 to attach

the camera.

Provision to upgrade for LED Fluorescence application in

future.

2) High quality Infinity Corrected System Objectives 10x Ph1,

20xPh1 and 40x Ph1, 100x dry and wide-field paired eyepiece

10x /20 mm FOV with focusable & dioptre adjustable

swivelling siedentopf tubes for comforting viewing angle.

3) Inter Pupillary Distance adjustment range 48-75mm. Intensity

settings to be easily displayed on both sides of the stand by

live LEDs as used as intensity indicator.

4) Large Specimen stage 200 mm x 239 mm to accommodate

different samples, large tissue culture bottles, petri dishes,

microtiter plates and culture flasks. Stage enlargment

accessory available. XY movement guide for stage and

holders for petridishes, 96 well plates should be available.

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5) Should be completed with filter for even homogeneous

illumination & Interference green filter for phase contrast

sample. Long working Condenser 0.4 with universal phase

slider for phase objectives & built-in aperture diaphragm for

bright field and phase contrast.

6) There should be automatic stand-by for illumination/ on/off

switch facility of light illumination ideal for 10-15 min. to

increase the life time of the illumination

7) Resolution upto 5.0 Megapixels & pixel size should be: 2.2

µm x 2.2 µm or higher, frame Rate maximum 38 fps &

complete with c-mount adapter

Imaging software for image capturing, live image preview,

white balance adjustment, interactive measurement, angel,

cantour, acquisition & annotations.

1) 27

2) Accessories for Upgradation

for with PlasDIC, Objective,

Imaging system for existing

Existi 'Carl Zeiss' Inverted Microscope

“Axio Vert A1-FL

Microscopy Camera Axio Cam ICc5 (Make: Carl Zeiss)

With AxioVision driver, configuration tool and connection

cable firewire CCD Sensor, 5.0 Megapixels, color, CCD,

Pixel size: 3.45 µm x 3.45 µm, Zeiss Axio vision Imaging

Software - Interactive measurement: length, contours, angles, -

Geometric scaling , Measurement bar, - Management,

visualization and printing of metadata - Image processing and

optimization (contrast, brightness, gamma, colors), Colour

adjustment, White balance

PlasDIC module LD A-Plan 10x-63x for contrast slider 10x29

mm

3 position contrast slider 10x29 mm for PlasDIC module and

analyzer

Analyzer module Pol ACR P&C for transmitted light

Slit diaphragm 3.5 mm PlasDIC for condenser (10x-40x)

Slit diaphragm 5 mm PlasDIC for condenser (40x/63x)

Slider PlasDIC for LD A-Plan 10x-63x

Analyzer module Pol ACR P&C for transmitted light

Objective EC Plan-Neofluar 100x/1.30 Oil M27

(WD=0.20mm), incl. Immersol 518 F, oiler 20ml and Cover

glasses, high performance, D=0.17mm, box with 100 pc.

Objective LD Plan-Neofluar 63x/0.75 Corr Ph2

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28

HIGH SENSITIVE

CONFOCAL

WORKSTATION WITH

HIGH RESOLUTION

ATTACHMENT

cell biology applications, cell lines, tissues. The system should

be available with the below mentioned configuration as

mentioned below:

A. Motorized Inverted Fluorescence Research Microscope:

a) Fully Motorized Inverted Fluorescence Research Microscope

for BF/DIC/Fluorescence preferably with dedicated touch

screen TFT display for controlling motorized components of

the microscope.

b) Mot. X-Y stage with Universal sample holders for slides, 35/60

mm Petri dish, labtek chambers with multipoint, tile and mosaic

imaging software.

c) LED / Halogen llumination for transmitted light & 120W metal

halide illumination for Fluorescence should be offered.

d) Motorized 6 position DIC nosepiece, Universal Motorized

Condenser NA 0.55 or better with modules for DIC, 6 position

fluorescence turret for accommodating fluorescent filters for

sample visualization.

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e) High precision Z-focus drive with step size of 15 nm or better.

f) High resolution confocal grade objectives of 10x/0.40,

20X/0.80, 40x/1.30oil, 60/63x/1.40oil immersion.

g) Automated shift free DIC accessories for all objectives.

h) Band pass fluorescent filters for DAPI, GFP, Cy3 should be

offered.

i) An active anti-vibration table with compressed air damping,

bread board table top with M-6 threading for the complete

microscope system.

B. Spectral confocal imaging unit with built-in high sensitive

detectors:

a) Laser point scanning and Confocal detection unit with 3

channels for simultaneous detection of 3 fluorophores.

Detectors should be capable of working in Intensity and

Spectral mode Imaging. System should be a combination of min

2 PMT + 1 GaAsP/HyD detectors in the scan head.

b) Scanner unit should have laser ports for 4 lasers to be integrated

with the system.

c) The scanner should have real"ROI" scan capability for fast scan

. Maximum scan resolution should be at least 4Kx4K or better

per channel and should reduce to 16X16 resolution.

d) Scan speed should be 7-10 fps or better @ 512x512 and should

be able to increase up to 50 fps@512X32 or better.

e) Transmitted PMT for laser based DIC imaging should be

included.

f) The scan field diagonal should be min 18 mm or better. Scan

Zoom range minimum 1X to 40X with increments of 0.1X.Scan

rotation of 360 degrees should be available.

C. Solid State Laser module with AOTF control:

a) 405 nm,

b) 488 nm

c) 561 nm.

d) 640 nm.

All lasers should be connected to the scan head through fiber

optic cable and should be controlled through AOTF for fast

laser switching and attenuation in pixel precise synchronization

with the laser scanner for Real ROI scan for FRAP, Photo

activation/conversion experiments. All the laser lines should be

controlled through a computerized AOTF device for fast laser

switching and attenuation.

D) High Resolution Imaging: a) Real-time and Online Fully automated and motorized HR

attachment with suitable high sensitive Detector for complete

Vis Spectrum.

b) Lateral resolution of 150nm or better and Axial resolution of at

least 400 nm or better should be expected out of the system.

c) Detection should be based on GaAsP or high sensitive

detectors.

d) Should be able to perform live cell SR Imaging . All laser lines

for Confocal Imaging should be used for imaging in SR mode.

F) Control computer and Monitor:

Latest 64 bit control computer with Intel Xeon Processor,

DDR RAM 32 GB HDD: 2TB SATA upgradable to 4 TB or

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better, DVD, SuperMulti SATA +R/RW, Graphics : AT Fire

GL V5200 256MB DH DVI, Gigabit Ethernet, Win 7 Ultimate

64 bit , USB 2.0, Fire wire. Large 30” LCD TFT monitor.

G) System control and Imaging Software:

a) Software should be capable of controlling Motorised

components of microscope, digital camera, confocal scan head,

laser control including AOTF and Image acquisition &

processing for confocal and super resolution imaging.

b) Saving of all system parameters with the image for

repeatable/reproducible imaging.

c) Line, curved line, frame, Z-stack, Time series imaging

capabilities.

d) Real ROI bleach for FRAP, Photo-activation/conversion

experiments.

e) Standard geometry Measurements like length, areas, angles etc

including intensity measurements.

f) Advanced 3D image reconstruction with rendering from a Z-

stack image series.

g) Co-localization and histogram analysis with individual

parameters.

h) Spectral un-mixing with fingerprinting for separation of

overlapping excitation/emission spectra of fluorophores.

29

Compact

chemiluminescence and

fluorescence gel and

western blot documentation

platform

full-feature instrument for imaging and analyzing gels and

western blots. Should be designed to address multiplex

fluorescent western blotting, chemiluminescence detection,

general gel documentation applications, and stain-free

technology imaging needs.

Automatic recognition of application-specific tray and

adjustment of imaging parameters and software options

Precalibrated focus for any zoom setting or sample height

Dynamic; precalibrated and optimized for each application

Mage area: 21 x 16.8 cm

Cool CCD camera, 6 MP

Trans-UV, 302 nm excitation

Epi-white

Trans-white (with White Sample Tray)

Trans-blue, 450−490 nm excitation (with Blue Sample Tray)

Epi-blue, 460−490 nm excitation

Epi-green, 520−545 nm excitation

Epi-red, 625−650 nm excitation

Epi-far red, 650−675 nm excitation

Epi-near IR, 755−777 nm excitation

590/110 nm standard filter to perform protein and DNA gel

and blot imaging

Chemiluminescence filter

518–546 nm filter for green-emitting fluorophores and stains

577–613 nm filter for yellow/orange-emitting fluorophores

and stains

675–725 nm for red-emitting fluorophores and stains

700–730 nm filter for far red–emitting fluorophores and stains

813–860 nm for near IR–emitting fluorophores and stains

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Portable microweighing

Balance

Specifications:

Capacity: 120g-10 microgram

Minimum display: 0.01mg

Pan size (mm): 110 dia

Power supply: two ways (AC or Battery operation).

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31

Balance Specifications:

Capacity: 32/180 Gms

Readability: 0.01mg

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Cryotome

Specifications:

Temperature Control Chamber: 0° to -35°C (at 20°C

ambient temperature)

Microtome: Specimen retraction 25,000 μm

Microtome: 1-20 μm (1 μm increments), 20-60 μm (5 μm

increments)

Ordering Alerts: XY orientation head is standard

Specimen temperature control, debris tray, Storage shelf,

Cryocassettes

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33

FULLY AUTOMATIC SOLVENT

EXTRACTION WITH HYDROLYSIS SYSTEM

Total Fat System: The system should be offered for Total Fat

solution which automates and simplifies total fat analysis. Consisting of an extraction unit, a hydrolysis unit and a single filter system that is common to both units, the Total Fat solution should perform Acid Hydrolysis and Solvent Extraction with one integrated procedure. The system should reduce the potential for human error in total fat analysis while reducing costs and improving overall sample throughput.

Extraction Unit: Fully automatic 6 sample solvent extraction system for Oil/ Fat extraction with 4 stage extraction using Randall procedure viz., Boiling, Rinsing, Solvent recovery and pre-drying steps. 6 sample extraction unit should have facility to expand to 12 position extraction unit & should be controlled by same control unit.

Extraction positions should have individual hotplate control.

Must be fully pre-programmable. Can set and store temperature and times for various steps.

Official approvals: AOAC, SCAN-CM, EPA

Fully automatic for unattended operations

Fully autoclavable liquid handling drive of 100ul to 5ml ( 4 ranges )

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Aerosol barrier protection device of 10ul/20ul/100ul/200ul & 1000ul (96x8)

Stirring motorless upto 1.5K capacity 1500ml

System should have Hydrocap filter which contains the sample from the initial weighing through hydrolysis to final extraction avoiding sample transfer errors. It should be a fully automated system that performs addition of acid, heating, boiling , rinsing and draining.

The extraction unit should have a speed dial for closed solvent addition for safe and consistent solvent addition so avoiding manual handling of chemicals.

The extraction unit should perform the four extraction steps boiling, rinsing, solvent recovery and auto shut down, fully unattended.

System should have extensive range of accessories with glass and aluminium cups and thimbles in different sizes to fit individual laboratory needs and applications.

System should quote with accessories comprising: 3 sets Extraction cups glass & Aluminum, standard 6/set each, 2 Thimble stands 26-33mm, 2 sets of Thimble adapters plastic 33mm 6/set, 1 set of Cellulose thimble 33 mm single thickness 25/set, Condenser Seal Viton/Butyl 6/set, Condenser Seal Resel 6/set, Condenser Seal Polyurethane 6/set

Minimum quantity of solvent required per extraction is 70 ml to 90 ml.

80% of solvent is recoverable at the end of the cycle.

Extraction time typical 45 min to 60 min . Extracting a batch should not be more than 60 minutes.

Capacity per batch should be 6

Capacity per day should be 42

Measurement range should be 0.1 to 100 %

Sample Size 0.5 gm to 2 gm ( for total fat)

Reproducibility should be +/- 1 % rel. or better ( 5 – 100 % fat)

Accuracy should be according to officially approved methods

Instrument should have 9 different programmable capacity

Power rating should be 1800 Watts

Temperature range 0 to 285 deg Cel. & heating up time for 20 – 285 deg Cel should be 5 min.

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Instrument should have Cooling water tab & should have water

flow rate 2 lit/min for < 25 deg Cel.

Ventilation: Fume hood. Mains power interlocked to the airflow which must be at least 0,5 m/sec.

Protection class should be IP 41

Batch handling tools for loading of solvent cups and thimbles are to be provided with the instrument.

Addition of solvent should be using a closed system, to eliminate hazard in the laboratory.

A safety door in front of the extractors is essential to ensure laboratory safety while using solvents.

Over temperature protection should be given at different levels of 145, 210 and 330 deg. C which can be selected depending on the solvent used.

34

Accessory for existing gel

doc system

Monochrome CCD Camera

16 bit 1.4 mega pixel resolutions and 4.2

megapixel image resolution.

Low noise. High speed USB data interface

Peltier Thermoelectric Cooling to -28 d.c. below

ambient

Pixel Size : 4.65µm x 4.65 µm

Full Well capacity : ~20000 e-

Read noise : <5 e- rms

Dynamic range : 4 orders of magnitude.

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35

Kjeldahl Digestion Unit

Amperage- 11A

Depth (English)- 18.25 in.

Electrical Requirements- 115V 50/60Hz

Height - 43cm

Width- 26cm

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36

1. Furnace System

(for Upgradation of Thermofischer AAS)

Furnace head

Furnace power supply

Furnace auto-sampler

Fixed mount

Normal Electrographite cuvettes (10)

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37

2. Semi auto Biochemical Analyzer

(Blood Biochemistry)

Method: Kinetic, End-point, Linearity end-point,Non-

linearity end-point,

Absorbency: Sampl blank, Multi-

standards, Velocity, Calculation, Fixed

Time, bichromatic etc.

Flow cell: quartz flow cell 32uL

With both shipping and cuvette system

Lamp: 6V 10W

Measurement range: 0~2.5Abs

Resolution: 0.001 Abs(display), 0.0001 Abs(calculation)

Temperature control: Peltier 37°C,30°C,25°C ± 0.1°C

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3. Ball Mill

With grinding bowl and balls

Number of working stations 2

Grinding bowl sizes 20, 45, 80 ml

Max. feed size (depending on the material) 5 mm

Min. sample quantity 0.5 ml

Max. sample quantity 70 ml

Final fineness (depending on the material) < 0.1 µm

Typical grinding time (e.g. quartz sand to < 40 µm) 4 min

Grinding process Dry/wet

Speed of main disk 100 – 1100 rpm

Transmission ratio i relative = 1 : –2

Effective diameter of main disk 140 mm

Centrifugal acceleration 95 g

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Interfaces USB, Bluetooth, Ethernet

Electrical details 100-240 V/1~, 50-60 Hz, 1200 watt

Bowl size- 20 ml

Ball diam- 5mm

39

BINOCULAR LABORATORY MICROSCOPE

Body: Aluminum die-cast body with all critical movements based on ball bearing & wire guides thereby ensuring smooth & precise manipulation Optical System: Optics par focal, infinity plan achromatic lenses with anti fungal properties and upgradable to EPI LED fluorescence. Illumination: • Built in transmitted Koehler illumination. • 6V30W Halogen illumination • work on voltage 100 V-240V AC. Focusing: Co-axial coarse & fine controls with a focus adjustment and fine adjustment knobs. Coarse Focus range 20mm. Fine focus range 0.2mm Revolving nosepiece: Quintuple revolving inward nosepiece based on precision ball-bearing mechanism with positive click stop Mechanical Stage: Co-axial low drive mechanical stage(125mm x145mm)(+/-5mm)with traverse area of 50mm x 76mm (+/-5mm) with dual side holder Condenser Holder: Rack & pinion mounted condenser holder Condenser: Center able abbe condenser with aperture iris diaphragm (N.A. 1 .25) focusable with rack & pinion through 20mm and a continuously variable iris diaphragm with a removable blue filter for day light observation. Objectives: Plan Achromatic Objectives N.A W.D 4X 0.10 25 mm 10X 0.25 5 mm 20x 0.40

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40X (spring loaded) 0.65 0.5 mm 100X (Oil, spring loaded) 1.25 0.14 mm lnfinity corrected plan optics Uniformly centered, interchangeable & Parfocal Anti fungus treated Tropicalised anti fungus treatment ensures image excellence for long periods in conditions favoring to fungus growth Inclined Observation Head: Binocular (30 degree inclined seidentopf), 360 degree rotatable, diopter adjustment Eyepiece: WF 10x (FN 20mm) paired eyepiece. The unique optical design of the compensating eyepiece provides relief from eye fatigue and renders color-compensated wide field images of utmost clarity.

Dr. Nachiketa Bandopadhyay Registrar