Softacid AquaBY-PRODUCTS preservation by acids applicationsZOOTEXNIA S.A. Address: 10 Nikomidias str. Peristeri, AthensP.O.: 12134 Tel.:+30 210 5766048Fax: +30 210 5766261 Email:zootex@otenet.gr | www.zootexnia.com

PRODUCTION PROCESS

By Product(70-80 % Moisture)

COOKER(Protein coagulation)Cooked cake(2-3% Moisture)PRESS(Liquid and fat release)FILTER

Pressed cake

Meat meal (sale)

Fat with suspended liquidsWaterSludge from tricanterWater residue (Treatment and disposal)TRICANTER(centrifugation)Transformation in category II

Oil (Sale)

Meat by-productBACTERIA FLORATimeParticle sizeTemperatureWater activityFree fatty acidsHIGH ENZYMATIC ACTIVITY(decarboxylase)Biogenic AminesAmmoniaEVOLUTION OF MEAT MEAL STABILITYBacteria load

Analytical valuesHigh Oleic acidityHigh Histamine levelHigh TVN Level

DIFFERENT CATEGORIES OF BIOGENIC AMINESHistamineTryptamineTryptophaneHistidineHetero CyclicTyraminephenylethylaminePhenylalanineTyrosineCyclicPutrescineCadaverineLysineOrnithineArginineDiamineSpermineSpermidinePolyamine

MEAT by-productTimeParticle sizeTemperatureBacteria loadFree fatty acidsBiogenic AminesAmmoniaBACTERIA FLORA

LOWENZYMATIC ACTIVITYOrganic acid environment Reduction of bacterial flora development

Reduction of enzymatic activityEVOLUTION OF MEAT MEAL STABILITYWater activity

Analytical valuesLow Oleic acidity levelLow Histamine levelLow TVN Level

Softacid AquaSlaughterhouses byproducts have a perfect nutritional and microbiological quality for the manufacture of meat meal.

Until they reach the factory of meat meal they are degrading. Factors: temperature, humidity, etc.

Slow down this process:COLD: PRESENT. Theorically in the slaughterhouseACID: FUTURE. Perfect preservations from 6 months 1 year.

Usual storage conditions before truck loading

Usual storage conditions before truck loading

SOLUTION IN NORWAYSILAGE (MILLING* of by-products with acid) from slaughterhouse by-products of salmon and trout. The product will be preserved during more than one month.

(*) Silage needs an important investment

SOLUTION IN CHILESILAGE (MILLING* of by-products with acid) from dead fishes. The product can be preserved during more than six months.(*) Silage needs an important investment

WE OFFER A NEW SOLUTIONDevelopment since 2011 with many trials in different factoriesPreservation of solid by-products from fish, meat and food industriesWithout coldWithout milling

TRIAL IN SPAIN / MARCH-JULY 2011Applying acids for the preservation of byproducts.We poured an acid almost at the same time that the production of byproducts in the silo.Comparative between two samples of 300 g of poultry intestines taken from the silo and analysed 20 hours later in the laboratory

SLAUGHTERHOUSE N 1(Data obtained from dry matter)WITH Softacid AquaWITHOUT ACIDpH3.495.74TVN (mg N/100 g chantillon)0.020.19OLEIC ACIDITY(%)9.453.6PUTRESCINE (ppm)43849CADAVERINE (ppm)161.062HISTAMINE (ppm)18683ENTEROBACTERS (ufc/g)< 1032.1 x 108

(* 1) fresh matter in truck.(* 2) Fat obtained after the manufacturing process of meat meal. (* 3) Power stop during the manufacturing process of meat meal.(*4) Lower pH due to retention of exudate

Sampling Days 14-april05-may12-may19-may26-may02-juneAcid dose (Kg/t)1010105510pH at the reception in factory (Higienizo result.) (*1)5,195,375,275,64,3 (*4)pH at the reception in factory (customer result) (*1)5,135,55,36,14,8pH in laboratory (*1)6,15,685,945,76,164,1Oleic Acidity (%) (* 1)30,312,76974,386,512,4Oleic Acidity (%) (* 2)444,453,77 (*3)TVN (g N2/100 g)0.2900,160,220,250,430,059Peroxids (meq/Kg)0,4490,126,420Formic Acid(%)0,240,150,340,25-0,89Putrescine (ppm)11671167136056104691142Cadaverine (ppm)13331330150663194775258Histamine (ppm)14514923629030073Enterobacters (ufc/g)3,5 x 1073,5 x 1073,5 x 1061,65 x 1083,5 x 1079,5 x 105

TRIAL IN PORTUGAL (February 2013)2 batches of by-products from poultry slaughterhouses.Temperature 10-15 C:

Intervalle collect/ Processing (hours)Oleic acidyControl53.5510Kg/T Softacid Aqua242.9

TRIAL IN PORTUGAL (May-June 2013)SLAUGHTERHOUSE CSLAUGHTERHOUSE A1SLAUGHTERHOUSE A2SLAUGHTERHOUSE A3SLAUGHTERHOUSE B1SLAUGHTERHOUSE B2SLAUGHTERHOUSE B3TRUCK A Acidification 25 T/day20 T/day25 T/dayTRUCK B No acidification 20 TAcidified25 T Non Acidified8.33 T/day8.33 T/day65% acidified8.33 T/day35% non acidifiedAcidified by layer in the truck Acidified in the mass

Remark: Acid application problem in Slaughterhouse C begin end of Friday 5 and during Monday 8 and Tuesday 9 (valve was broken down)Good control with 65% of treated by-product but not sufficient with only 35%After the trial, Truck B also equiped to tread the by products of Slaugher houses B1 B2 and B3 (30-40 C at noon)TRIAL IN PORTUGAL (May-June 2013)

Acidification Day Daily Average Slaughter Houses B1 B2 B3Slaughter house A1 A2 A3 Truck ASlaughter House CMonday 1 6,4 Slaughter Houses B1 B2 B3Slaughter house A1 A2 A3 Truck ASlaughter House CTuesday 2 4,4 Slaughter Houses B1 B2 B3Slaughter house A1 A2 A3 Truck ASlaughter House CWednesday 3 4,9 Slaughter Houses B1 B2 B3Slaughter house A1 A2 A3 Truck ASlaughter House CThursday 4 4,6 Slaughter Houses B1 B2 B3Slaughter house A1 A2 A3 Truck ASlaughter House CFriday 5 6,8

Acidification Day Daily Average Slaughter Houses B1 B2 B3Slaughter house A1 A2 A3 Truck ASlaughter House CMonday 8 11,1 Slaughter Houses B1 B2 B3Slaughter house A1 A2 A3 Truck ASlaughter House CTuesday 9 9,3 Slaughter Houses B1 B2 B3Slaughter house A1 A2 A3 Truck ASlaughter House CWednesday 10 5,2 Slaughter Houses B1 B2 B3Slaughter house A1 A2 A3 Truck ASlaughter House CThursday 11 5,1 Slaughter Houses B1 B2 B3Slaughter house A1 A2 A3 Truck ASlaughter House CFriday 12 5,7

Resullts on Oleic acidity during the 4 summer months * This value is the average value of the offal Monday-Tuesday** The offals of this week was rejected as unacceptable

JuneJulyAugustSeptember20122013201220132012201320122013Monthly average9,95,89,76,19,57,38,16,4First week9,26,813,75,110,66,59,56,2Second Week10,74,67,67,4 *12,98,087,1Third Week8,85,99,85,38,6 **-8,45,9Fourth Week11,95,77,65,37,2 **7,2--

TRIALS IN MOROCCO (A - January 2013)120 T of fish by products on thursdayProcessing on monday morning (84-107h)Time elapsed untill the fish was processed was 84-107 hours.

Histamine ppmControl1700Softacid Aqua 3.5 Kg920

TRIALS IN MOROCCO (A February 2013)163 tons of fish and around 12 tons of byproducts. Acid application on Sunday and the by products were delivered to the meal factory during an average tranportation time (10-11 hours). Fishes and fish byproducts were processed the following day.

Histamine ppmIncrease vs FrozenNo acidification1000X 10.5Softacid Aqua10 Kg/T295X 3.1Frozen fish (Control)950

TRIALS IN CHILE A. November 2012A test took place applying acid over trout fishery sludge in order TO ELIMINATE THE BAD ODOURS. The composition of the sludge was about 50% faeces, 25% water, 15% undigested feed and 10% fat. Average temperature of the test was 20-25 C.

In both acidified samples the smell dissapeared in less than 60 seconds. (As close as 30 cm from the sample, no odour is detected)TRIALS IN CHILE A. November 2012

Samples of 100 Kg0 days60 days90 daysNON acidified samples5,46.06.0Acidified samples (35 l/ton)3,33.33.4

Samples of 1000 Kg0 days60 days90 daysNON acidified samples5,46,56,5Acidified samples(35 l/ton)3,33,33,5

B. TEST IN SALMON SLAUGHTERHOUSE, OVER SALMON INTESTINES (50 Kg cu)Batch 1: 50 Kg of salmon guts WITH NO ACIDSBatch 2: 50 Kg of salmon guts ACIDIFIED with 10 Kg/Tm of Softacid Aqua.

ResultsSample identificationHistamineCadaverinePutrescineOleic acidity %TVN mg N/100gpH %T0 %With treatment Time 0Without treatment Time 068247133572345,702,8914,6813,306,493,2615,216,5With treatment Time 6Without treatment Time 61011973723123277,713,1731,2311,536,253,4617,616,5With treatment Time 12Without treatment Time 121111785825303268,492,9944,0613,536,293,6319,918,4With treatment Time 18Without treatment Time 181461894233423269,783,1456,9813,366,263,7819,918,5

SOFTACID AQUA APPLICATION IN THE SLAUGHTERING HOUSESpraying system in factory

SOFTACID AQUA APPLICATIONIN THE TRUCK

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