Upload
others
View
6
Download
0
Embed Size (px)
Citation preview
En
SpecificationsInverted Microscopes
TS100 (Binocular model) TS100-F (Trinocular model)
Optical system CFI60 infinity system, parfocal distance 60mm
Main body
Main body Square box type with both-end support stage
Focusing Vertical objective movement
Coarse stroke: 37.7mm per rotation, Fine stroke: 0.2mm per rotation
Eyepiece tube Siedentopf-type binocular tube Siedentopf-type trinocular tube (light distribution, bino/photo: 100/0, 0/100)
Interpupillary distance: 50–75 mm, Eyepoint height: 400mm from table, Inclination: 45˚ from horizon
Nosepiece Quintuple nosepiece, backward-facing type
Plain stage Stage size: 170 x 225 mm, Stage height: 195mm from table, Auxiliary stage attachable
Illumination Pre-centered 6V-30W halogen lamp, Filter frame (accepts 2 filters), Heat absorbing filter and diffuser
Slider Non-centerable phase slider (PhL, Ph1, 1 empty position)
Centerable phase slider (PhL, Ph1, 1 empty position), Ph2 ring (optional)
NAMC slider (NAMC1, NAMC2, NAMC3)
Attachable mechanical stage Stage movement: 126 x 80 mm
Accepts several micro-testplate holders
Holder φ35mm Petri Dish holder, Universal holder, Terasaki holder (accepts ø65mm petri dish), Slide glass holder(accepts ø54mm petri dish), Hemacytometer holder
Filter 45mm NCB11, ND8 and GIF (green interference)
Eyepiece lens C-W 10X (F.O.V. 22mm), C-W 15X (F.O.V. 16mm)
Condenser (without ELWD condenser: N.A. 0.3 (O.D. 75mm)condenser O.D. 190mm) NAMC condenser: N.A. 0.4 (O.D. 44mm)
Epi-fluorescence attachment Field diaphragm, Fluorescence filter block holder (2 filter blocks mountable, 1 empty position), Heat absorbingfilter, Lamphouse for 50W mercury lamp, Light shielding plate, UV-cut filter (detachable)
WARNINGTO ENSURE CORRECT USAGE, READ THE CORRESPONDINGMANUALS CAREFULLY BEFORE USING YOUR EQUIPMENT.
Printed in Japan (1003-06)T Code No. 2CE-MQLH-2
Specifications and equipment are subject to change without any notice or obligation onthe part of the manufacturer. March 2010.
©1999-10 NIKON CORPORATION
This brochure is printed on recycled paper made from 40% used material.
NIKON CORPORATIONShin-Yurakucho Bldg., 12-1, Yurakucho 1-chomeChiyoda-ku, Tokyo 100-8331,Japan (from May 6, 2010)phone:+81-3-3216-2375 fax:+81-3-3216-2385 http://www.nikon.com/instruments/
NIKON INSTRUMENTS INC.1300 Walt Whitman Road, Melville, N.Y. 11747-3064, U.S.A.phone: +1-631-547-8500; +1-800-52-NIKON (within the U.S.A. only)fax: +1-631-547-0306http://www.nikoninstruments.com/
NIKON INSTRUMENTS EUROPE B.V.Laan van Kronenburg 2, 1183 AS Amstelveen, The Netherlandsphone: +31-20-44-96-300 fax: +31-20-44-96-298http://www.nikoninstruments.eu/
NIKON INSTRUMENTS (SHANGHAI) CO., LTD.CHINA phone: +86-21-5836-0050 fax: +86-21-5836-0030(Beijing branch) phone: +86-10-5869-2255 fax: +86-10-5869-2277(Guangzhou branch) phone: +86-20-3882-0552 fax: +86-20-3882-0580
NIKON SINGAPORE PTE LTDSINGAPORE phone: +65-6559-3618 fax: +65-6559-3668
NIKON MALAYSIA SDN. BHD.MALAYSIA phone: +60-3-7809-3688 fax: +60-3-7809-3633
NIKON INSTRUMENTS KOREA CO., LTD.KOREA phone: +82-2-2186-8410 fax: +82-2-555-4415NIKON CANADA INC.CANADA phone: +1-905-602-9676 fax: +1-905-602-9953NIKON FRANCE S.A.S.FRANCE phone: +33-1-4516-45-16 fax: +33-1-4516-45-55
NIKON GMBHGERMANY phone: +49-211-941-42-20 fax: +49-211-941-43-22
NIKON INSTRUMENTS S.p.A.ITALY phone: +39-055-300-96-01 fax: +39-055-30-09-93
NIKON AGSWITZERLAND phone: +41-43-277-28-67 fax: +41-43-277-28-61
NIKON UK LTD. UNITED KINGDOM phone: +44-208-247-1717 fax: +44-208-541-4584
NIKON GMBH AUSTRIA AUSTRIA phone: +43-1-972-6111-00 fax: +43-1-972-6111-40
NIKON BELUXBELGIUM phone: +32-2-705-56-65 fax: +32-2-726-66-45
Coarse/fine focus knobThe coaxial coarse/fine focus knob, located infront of and close to the operator, makesoperation at high magnifications more efficientand convenient than ever before.
Efficient, user-friendly stageThe stage features a low-profile design that is195mm high, making itthe ideal size for a labbench or safety hood.Even cell cultures on thebottom of a tall flask orstacking chamber vesselcan be viewed, becausethere is 190mm of spaceabove the stage when thecondenser is removed.
Eclipse TS100.Adding new dimensions to
inverted microscopesIn designing the new microscope, Nikon started with its
optical performance. First, they incorporated theiracclaimed CFI60 optical system—a fusion of CF optics with infinity optics—into this new, small-sizedinverted microscope. These optics provide flat, sharp,and brilliantly clear images, while achieving longerworking distances and higher numerical apertures.Furthermore, epi-fluorescence and NAMC observationsare now possible using accessories available as options.To improve observation under phase contrastmicroscopy, Nikon developed a series of ApodizedPhase Contrast objectives, allowing minute details
within a specimen to be observed with excellent contrast and wider tonal ranges.But Nikon didn’t stop here. They redesigned the body, so that it is robust, rigid, andvibration-resistant, and placed all controls so that they fall naturally under your hand. To accommodate image documentation, Nikon offers a trinocular model as well. TheTS100-F comes with a photo port and accepts various photomicrographic systems,including a CCTV camera, or a digital still camera.
CFI 60 objectives
Binocular type Model TS100
Operation is simpler, quicker, more precise,because there is less strain on the user
Easy-to-rotate nosepieceThe quintuple (5-position) backward-facing nosepieceoffers plenty of clearance to allow the operator to rotateit from either side. Because there is ample spacearound the nosepiece, handling the nosepiece is easy,even for an operator with large or gloved hands.
Eyepiece tubeThe Siedentopf-type eyepiece tube is inclined 45˚ and theeyepoint height is 400mm for easy, comfortable viewing inthe sitting or standing position.
Tube inclination
45˚
Eyepointheight
400mm
Comfortable operation
3
Plenty of clearance around the nosepiece
Trinocular type Model TS100-F
Acrylic stage ring set
Objective in use is easily identified throughthe transparent stage ring.
Transparent stage ringTwo types of acrylic stage rings come with the mainbody. Because these stage rings are transparent,confirming which objective is being used is easy. The ring with the semicircular hole facilitatesobservation of the specimen in a chamber since itprevents the objective lens from striking the ring duringmagnification changes. A glass stage that minimizesthe possibility of thermal deformation is also availableas an option.
EyepiecesFeaturing a 22mm field of view, the widest in this class of microscope, the TS100/TS100-F ensures clear images up to theperiphery of the field of view even when using higher magnification objectives.
190mm
Ample space above the stage
Observation methods that provide the most informati on from your specimens
Epi-fluorescence method
This method is ideal for identifying fluorescent tagged substanceswithin a cell, green fluorescent protein (GFP), and a myriad of otherclinical and research applications. Epi-fluorescence observation utilizing UV-range light is also possible.
This method is now possibleeven with a microscope of this class. NAMC creates vivid, 3-dimensional-like images of living, transparent specimens, allowing observation inplastic petri dishes—something that DIC does not do well.
Nikon Advanced Modulation Contrast method
Epi-fl attachment
NAMC condenserTS100 configured withNAMC set
4 5
T lymphocyte cell (GFP)
Breast cancer screening (DAPI)
q CFI Plan Fluor DL4X (N.A. 0.13, W.D. 16.4mm) PhLw CFI Plan Fluor DL10X (N.A. 0.3, W.D. 15.2mm) Ph1e CFI Plan Fluor 10X (N.A. 0.3, W.D. 16.0mm)r CFI S Plan Fluor ELWD20XC (N.A. 0.45, W.D. 8.2–6.9 mm)t CFI S Plan Fluor ELWD40XC (N.A. 0.60, W.D. 3.6–2.8 mm)y CFI S Plan Fluor ELWD ADM20XC (N.A. 0.45, W.D. 8.2–6.9 mm)u CFI S Plan Fluor ELWD ADM40XC (N.A. 0.60, W.D. 3.6– 2.8mm)
q w e
y utr
q w
q CFI ACHRO NAMC 10X (N.A. 0.25, W.D. 6.2mm)
w CFI ACHRO LWD NAMC 20XF(N.A. 0.4, W.D. 3.1mm)
e CFI ACHRO LWD NAMC 40XC(N.A. 0.55, W.D. 2.7–1.7 mm)
Trichuris trichiura egg
Hela cells in tissue culture vessel
TS100 configured withepi-fl attachment
Phase contrast method In addition to the conventional method, the newbreakthrough “Apodized” method is now available
Nikon has successfully reduced image halos by using a process called“Apodization” to improve the phase ring of the objective. Thisimproves vision during phase contrastmicroscopy by removing unwantedhalos to make it possible to more clearlyobserve cell division activities within aspecimen and view finer details within athick specimen.
The Principle of Apodized Phase Contrast MicroscopyIn the conventional phase contrast method, direct light* that has been weakenedby passing through a phase ring is made to interfere with diffracted light**,causing a phase shift and increasing image contrast.The new Apodized method utilizes the property of diffracted light in which adecrease in specimen size results in a greater angle of diffraction. Two absorbingbands with different transmittance have been added either side of theconventional phase ring DL toreduce halos and increasecontrast in the minutestructure of the specimen.
*Light that travels retaining theoriginal incident angle.
**Light that has been diffracted bythe specimen Phase ring DLPhase ring ADL
Absorbing bands
Phase ring
ELWD condenser and phase sliders
ADL, ADM objectivesq CFI Achromat ADL10X (N.A. 0.25, W.D. 6.2mm) Ph1w CFI Achromat LWD ADL20XF (N.A. 0.4, W.D. 3.1mm) Ph1e CFI Achromat LWD ADL40XF (N.A. 0.55, W.D. 2.1mm) Ph1r CFI Achromat LWD ADL40XC (N.A. 0.55, W.D. 2.7-1.7mm) Ph2t CFI S Plan Fluor ELWD ADM20XC (N.A. 0.45, W.D. 8.2-6.9mm) Ph1y CFI S Plan Fluor ELWD ADM40XC (N.A. 0.60, W.D. 3.6-2.8mm) Ph2
q w e r t y
Monkey kidney: CFI LWD ADL20XF
TS100 configured witha phase contrast set
Apodized phase contrast
ADL objectives for Apodized phase contrast
Monkey kidney: CFI LWD DL20XF
Phase contrast
DL objectives for phase contrast
e
Accessories to expand your capabilities System Diagram/Dimensions
Mechanical stageBy attaching appropriate holders,various specimen slides and microtestplates can be mounted on thisstage.
Specimen plate holdersThese specimen holders are availablefor use with the mechanical stage:q Hemacytometer holderw Terasaki holder (accepts ø65mm petri dish)e ø35mm petri dish holderr Slide glass holder (accepts ø54mm petri dish)t Universal holder
Auxiliary stagesFor large specimens, you can widenthe space on your plain stage byattaching a pair of auxiliary stages.
MicromanipulatorsThe Eclipse TS100/100-F can beconfigured with Nikon/Narishigemicromanipulators andmicroinjectors for a variety ofapplications, including injections,aspiration, and incisions of celltissues during cytoengineering,developmental and geneticengineering, electrophysiology,pharmacology, reproductivemedicine, and neurochemistry.
TS100-F configured withDigital Camera DS-Fi1-L2
With a CCTV camera
CCTV adaptersThese CCTV adapters are available as options:• C-mount TV adapter 0.6X—recommended for 2/3" CCD camera*• C-mount TV adapter 0.7X—recommended for 2/3" CCD camera• C-mount TV adapter 0.45X—recommended for 1/2" CCD camera*• C-mount TV adapter 0.38X—recommended for 2/3" CCD camera*• C-mount TV adapter VM4X**• C-mount TV adapter VM2.5X**• C-mount TV adapter A• C-mount TV adapter used with Relay Lens 1X*• ENG-mount TV adapter 0.6X—recommended for 2/3" CCD camera*• ENG-mount TV adapter 0.45X—recommended for 1/2" CCD camera*• ENG-mount TV adapter used with Relay Lens 1X*
* V-T photo adapter is neccessary** C-mount TV adapter A is neccessary
Halogen Lamp 6V-30W
Centering Telescope
C-W 10X Eyepiece
C-W 15X Eyepiece
Centering Telescope
C-W 10X Eyepiece
C-W 15X Eyepiece
CFI Objectives
Halogen Lamp 6V-30W
CFI Objectives
TI-FM Epi-fluorescence Attachment (cassette holder)
Epi-fl Filter Blocks
TI-SPH2 Phase Ring Ph2
TI-SNCP Non-centerable Phase Slider
TI-SCP Centerable Phase Slider
T1-C NAMC Condenser
(with slider)
TI-CELWD ELWD Condenser
TI-SM Attachable Mechanical Stage
Auxiliary Stages (paired set)
C-HT Terasaki Holder
C-HU Universal Holder
C-HSG Slide Glass Holder
Hemacytometer Holder
φ35mm Petri Dish Holder
C-LHG Mercury Lamp HG-50W
C-HGFIA HG50W Adapter R
HG Precenterd Fiber Illuminator Intensilight C-HGFI
C-SHG Mercury Illuminator 50W(Lamphouse)
C-SHG Mercury Illuminator 50W(Starter)
C-mount TV Adapters 0.38X, 0.45X, 0.6X
CFI Projection Lenses PLI 2X PLI 2.5X PLI 4X PLI 5X
C-mount TV Adapter A
V-T Photo Adapter
C-mount TV Adapters VM2.5X, VM4X
TI-FM Epi-fluorescence Attachment (main body)
CCTV Camera35mm SLR
Camera
F-mount Adapter
Epi-fluorescence Attachment
Main Body
Main Body
Stage Ring
Stage Ring
C-0.7XDXM Relay
Lens
C-mount Adapter
0.55x
400
318
480
140
150
227.5
400
480
126341 52.5
130
140Unit : mm
150
227.5
126341 52.5
130
System Diagram
Dimensions
6 7
Photomicrographic systems including a CCTV or digital still camera
The TS100-F comes with a photo port thataccepts photomicrographic systems such asthe DS-Fi1-L2, a stand-alone type digitalcamera with which you can take photoswithout PCs. Also a CCTV or photomicrographicequipment can be attached.
q
e r
t
w