STUDENT DESIGNED DNA

EXTRACTION LAB

STUDENT DESIGNED DNA EXTRACTIONQuestion: Can we extract DNA from

_____________________________________?

Hypothesis: If we try to extract DNA from _____________________________________ then we will be able to get DNA out of ______________________________________ as measured by whether or not we see the white DNA in the test tube

Introduction: ______________________________________________________________________________________________________________

DNA EXTRACTION NOTES (LINED PAPER)

MECHANICAL BREAK DOWN OF CELL

Smashing fruit in the bag = mechanical break down of cell wall

CHEMICAL BREAK DOWN OF CELL10mL Extraction buffer

contains:

1 mL Soap & 1/8 tsp Salt- breaks down lipids (fats) in cell membrane & nuclear membrane

A dash of Meat Tenderizer- breaks down proteins

CHEMICAL SEPARATIONCold Alcohol-

DNA does not like to be in alcohol, so it bunches up and precipitates out of solution

DNA EXTRACTION EXPERIMENT This is a qualitative experiment (no

graph) you will be measuring whether or not DNA was successfully extracted from each material during multiple trials (may need to modify each trial to see what works best)

Think about procedures that will be different based on your materials (plant or animal cell? So what about mechanical break down?)

MUST BE APPROVED TODAY Group must be stamped for question &

hypothesis TODAY (Pre-AP: must have procedures stamped today, too)

You will make your own extraction buffer, must calculate total amount required. Recipe below:Recipe for 10mL solution:10mL H2O1mL dish soap1/8 teaspoon salt1 pinch of meat tenderizer

Work on DNA crossword puzzle once approved

CLOSURE Draw a picture of the lab set up for one

of your trials in the DNA lab. Label all materials and amounts.

PROCEDURES & DATA TABLE1. Fix procedures

2. Copy onto template

3. Set up data table

4. Come get approval stamp for procedures/data table

5. Work on crossword puzzle to review for quiz

CLOSURE Draw a picture that visually represents your

hypothesis for your DNA extraction experiment

IF YOUR GROUP IS APPROVED THROUGH DATA TABLE (3 STAMPS TOTAL)… Come show Ms. D to head into lab!

Do NOT pour anything back into front stock bottles (extraction

buffer, alcohol). Take only what you need.

ONCE YOU ARE FINISHED1. Complete a 2-3 paragraph conclusion

for your experiment.

2. Staple together & turn in today (order= lab template, conclusion)

3. Complete the DNA worksheet

4. If you finish everything early…play DNA review games with partner!