99

100 Savita Kuldip Kumar et al./ Asian Pacific Journal of Reproduction (2013)99-104

101Savita Kuldip Kumar et al./ Asian Pacific Journal of Reproduction (2013)99-104

7

6

5 1B

A

23

7

5

7

362

7

7

31A

46

B

C D

6

2

100

80

60

40

20

0

PROLACTINMG/ML依sem

Control 1 mg HPL 2 mg HPL 5 mg HPL

102 Savita Kuldip Kumar et al./ Asian Pacific Journal of Reproduction (2013)99-104

Table 1Effect of Haloperidol (HPL) on the duration of various stages of oestrus cycle in albino rats.

Treatment and dose Duration of stages of the oestrus cycle in days (Mean 依 SEM)

Proestrus Oestrus Metoestrus DioestrousControl Saline 2 ml/kg/day,i.p. 2.33 依 0.22 4.00 依 0.00 2.50 依 0.00 7.33 依 0.22Haloperidol 1 mg/kg/day, i.p. 2.00 依 0.00 3.66 依 0.21 2.33 依 0.22 8.00 依 0.33Haloperidol 2 mg/kg/day, i.p. 1.66 依 0.22* 2.50 依 0.22** 2.00 依 0.22 9.33 依 0.16**

Haloperidol5 mg/kg/day, i.p. 1.00 依 0.00** 1.60 依 0.22** 1.66 依 0.00** 11.50 依 0.44**

Observation period= 16 days,* P<0.05, **P<0.01 as compared to control group.

Table 2Effect of haloperidol (HPL) induced hyperprolectinaemia on ovarian follicle development (n=6 in each group). Type of follicles Control 1 mg / kg/day 2 mg / kg/day 5 mg / kg/dayPrimordial & primary follicles 150 依 5.79 90.83 依 3.75** 10.16 依 1.77** 50.16 依 2.88**

Secondary follicles 57.50 依 3.07 32.33 依 5.83** 27.66 依 1.76** 95.16 依 6.18Early antral follicles 6.50 依 0.00 12.50 依 2.67** 4.66 依 1.20 1.33 依 0.61Follicles with clear antral space 2.50 依 0.42 5.16 依 4.38 2.33 依 0.49 5.66 依 0.80Largest follicles with zona pellucida 24.16 依 3.27 3.66 依 0.76** 7.33 依 0.73** 9.83 依 2.04**

Oocytes with multylayered zona pellucida 9.16 依 2.14 12.16 依 0.72 12.00 依 0.73 3.50 依 1.17*

Data were expressed as Mean 依 SEM, *P<0.05, **P<0.01, compared to control (Dunnett Comparison Test).

Duration of stages of theoestrus cycle in daysProestrus

Duration of stages of the oestrus cycle in daysOestrus

Duration of stages of theoestrus cycle in daysMetoestrus

Duration of stages of theoestrus cycle in daysDioestrous

Control

Salin

e 2...

HPL1 mg/k

g/day,

I.P

HPL2mg/k

g/day,

I.P

HPL5mg/k

g/day,

I.P

Type of follicles

Primordial & primary follicles

Secondary follicles

Early antral follicles

Follicles with clear antral spaceLargest follicles with zona pellucidaOocytes with multyalyered zona pellucidaOocytes with multylayered zona pellucida

103Savita Kuldip Kumar et al./ Asian Pacific Journal of Reproduction (2013)99-104

104 Savita Kuldip Kumar et al./ Asian Pacific Journal of Reproduction (2013)99-104